Lsi05G003300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G003300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein PTST, chloroplastic
Locationchr05: 4218219 .. 4223011 (+)
RNA-Seq ExpressionLsi05G003300
SyntenyLsi05G003300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTTTGATCTTTGTGGCTGGGACCGTACCTCTAGGCATGTTATACTTGACAAACAGAATTTTCTTTTGACTGTCAAATGTTTGTAAATTCAGAGACCACCTTAAAGAAGTTGTATTTCTGGAAAGGAATATCTTGGATTTTCTCAGCTCTATCAGGGAGGCCGAGAGAAGTACTTTGTTTACATTGAGAAACAAGAGTGAAGGCTTTCAAATAGATCCACACAAAAGAAAGTCTTTCACCGAAGAGAGAAGAGGTCTAAATACATATGGCTTTTCATTTCTTTTTTGAGTCAAATGTTCTTTTGAGGAACTCCATTTCTTATTTTCTTGGTATTCTTTTCTGTGGCTAATGTTATGTTTACATTAGAATTTTCATATGTACTTTGATAGTTCATGATTTTCTTGTTCTTCATGATTTTCATGATTTTCGTATTTACTTTGATAGGAGCCTGCCAATATGGAGATTTGTGCTCGAAGGTACTTATTCTTTTCTATACGAGAAAATTGTTGTTATGAACCTCTTAATATCTTGTTGACTTGAGCTTCAGTTATAGATTGCTGGCTCTAATTTTTGTGCCCCTTCCTGAAAAGGTTTGATGATTATCTCTTCATCTGGAACTTACTTGAAGCAATGTATCAAGAAGAAAGCATGCTCTGTATTTTTCGTTTTCTCTTCTTTTTAAATTTTGTTTCAATCGAAAGGGGTTTAGGTTATCGTCTTTAATATGGATGTGATTAATGCAATTTTCCATTATGTCTATAATCATGAACAATTGAACATTATCTGTTAGTTCCAAATCATTATTTTCACTACGTTTGAAACTTTCTGAGAACCTGATGCCATTATTACTTATTTTATAATCTTGGTTAGGACTTGTCTGGACAACCAAGTTTTACTGAGTTCCAAGAATGCAAGAAAGTTAAAGATGGAAAACTTGAATAACCCTTCATGTAATATGGCAACCTGGAGCTTTAGGATGGGTTCTTGCAAGTTGATCCATTCTCATGAACCACCTGTAACCACACTTCCTCAAATGTATCGGATTCACAAAAGATATTCCTTGTCTGTCACATCAGAGGAATCTTCAATGCAATCAGAAGATCTTTCTACAGACGAAGAGGAAATTGTGAGTGCAGAGGAACTTCTGGCTCAACCCCTTAGTAGTGAACAGGTAAACGTAGACATTAAGTTCATAATTTAATGCGTTCGTGATACAGAATGAAGGATACTGCATTTATAATCTGCTGTTGTGACTGAGAATGAAATCAATTGCCACCTATTTCTTATTTTTGCTCGCTCAAAATCTTTCATGTAAAGTTTTAGCCTTCTTTTCCAAAACTCCTTCTTTTCCAAAACTTACAGTGTCCTCATTTTCACTATTTCTTTTAGCCTAAGTTATTTCTATCCTCAGTGCCGACTTTTCAACACAGTGTAGTGGTGTTTAGTGATTGATGCTCCTTTGCCATTTTGGAAAATAAAAGTTACTGGTTCTTGTCAGGCTTACATGATTTCCATACCTATCTTCAGAGAAGGTGGGGAATGTTGAGCGGGGAGATAAAAGAAAATCAACAGGGTTACCATTGTTTAGGAATTTAAAAGCTTCTGGTTTTTTGGAAAGACTTCAAGAATGTTAGTGACAGTGGGTGTCTATTGGGGAAGGATTTGGTATCATGGGTTCAAACTTTGGGCCACCTACTTACATCCTCAGTCTAACTTTGTAGGGTTAGACAAATTCTCTTTCTAAATTAGTTAAGGAATACTTAAGCTAATTTAGACAAGCTATGATTATCCAAAAAGGGAAAGTTTACATTTGTAGGAGAATAGAAAAATGGAAAAAAGAATAAAATACTGTAAAGGAACATATAGATATACTGAGCTTCAAAAAAATTGTCTCAGTATGAATGAATCTCTAAAAATTTTGGTATGCTCCATTCTTTTACTTACAATTTCTTGAATAGATTGTAGTGTTATGTAGACGAGAATCCTACTATTGCTGAAAACAAAACATAACAACTGATCGTGTAAAATATGTGAAGTGATCACAGCTTGGATAAACAAATAATGTTATGTAGAAAGCAATCAAAGCTGCATTGGCATTTCTTCAAAGCTTTCCTTCCATTCTGGAATGTTTCTTTCCGGAGAGCTTGATATCAAGCTTTTTTGTCTGTCAAAGATCTGAATGATATATAATTGACTACAGCTGAATGCATTACTGGCTGATTCAGAAAGAGAGAAGCTTGTCAAAAAGCTAAGTCACGCTAATCAGCAGAACAGACTTCTCAAACGACAGGTGTAAAGACATGATTTTTCTTTTTTTACTTTACAAGTATGTTAACCAAAGTTTTTACTCTTCATTATGCAGATGCAGACTCATTTTGCTATTATTATTTGTTTTTATGATCATGTTTTATGTTTACTATATCTTCTATTGTACTGGTTTTATATGGTGCAGATTAACTTAGAAACCAGAATCAAAATTCTATTAATAAATCTATTTGTCTCCAATTTATACTCAAATCTCGAATATTGGTCACAAAAATGTGCATATATTAACTTTTTAGTTAATTTTTATTTAATATGTGATAGGTTGAGTTATGAGTTTAGTTCCTCATCCATAGAACAATATTTCTTGACGTCCCTTTTTGCTCTGGTATTAATCTGTCCAGTCTTTTTTTCCTAATGTTAATTTGTCTTTCTGTTCTATGACCTATGTCAAGTAAATCATTTTTGTTGAGAATTATTAACAAAAGAATCCACCTGAAATAGATCGTTAGTCAAAGAAGAAATTCTGCTTCTTGCTTTCTTTTGTTCGATTAATGTCTGCAATAAATTAAGCTTTTCTATCAAGTTCATATTCCGTGGCATAATGACATCTTACTCTGAGGCATCTATATGTTTAGAACTCGTGCAATCATGTTTCTTTTCCCTTATCAGTTGTAAAATGTTCGAGTTAGACAGAGAGAAGAAAAACAAAATAATTCCTGAAAAGAGCTACAATATCTATCTATCCCTTGTAAAGCTTTCTTTATTATTTCAAGACAACTTTCCCATTAGATATATCTAAAGGCATTAAGCCACAAGGATTTGACATTTCGAATATTGAATCCTGTAATTTTTATATGTTGGCCCCTGGGAGTTGTAGATGTTTAGTGCGTTCTTATTTCCTTGATTCTTTCAAGACATAATAATTGAGGGACATGAAGTTAATAAGTATGGCCAAAATATTTGGTATCAAGGGGGAACAAAAAGTCTAAATCATATTTTATCTTCTCAGCTACATGTAAAGGATGAAGATTTAGTTAACTGTAAGACCGAACTTGCTGCCTTGGACCATGAAATTCAGGTTAGGCTTTTAACCTAACCCTTACATTTATTCAGATAATAAACACATATAGTTGTAATCTTCTTTCATGTATGGATATGTAAAGGGTTTGATCAAACTGGCAGAAGAAATAGCACAATCAGGCATTCCAGAAGGTACGAGAAAAATTAATGGGAAATACATTCAATCTCACCTCCTTACAAAATTAGAAGGTATAATGACGATCCTAACCTTTGTTTTCAACAATAGTGGGTTTCTTCCTTTGCACCTGAGTTTTATCGCCTTCTTTCTGCACTTGTATGTGAGATGATGTGTGCTGCCCTCCATGAAAGTTCTCCATTATTACTCAAAAATATATTGTTGACTTATTATTTTTTGGACTCTTCTTTTTCTTTGAAGCTGTACATAAAAAGCTAGAGGATCAAATAAAAGATGTAGATTTGGTGCAATCAAAAGAAGTTCCACTATTCTGGTATGGAATGGCTGAGGTAAGAACAGAAAATTCTATATTTGTTGAAGTATTGATATAATTAAATTTACTATAACCCATCAACTTAAGTTTTAAGATTGATTGGTGATTTAACAATATTTGTTACAATTTTCTCCCTACTCTGAAAGGAAACTAGCTTTCTACATTCTAATATTTGACTTTTACAATTTTCCTAAATGTCAAATGTTTGAATCCTTACCCCCACACTATTTTGAGAACAAAAACAACTAGGTCTGATTTTATTTTATTTTTTAAGTTTCTTTCTTTCATCATTGCCATCATTCTTATAATTTTATATTCATCTTTGAATAGAGTGTGCAAGTTATGGGAACTTTTGATGGCTGGAGTGTAGGGGAGGAGTTGTCACCTGAATACACTGGGGCTTACTCTAAGTTCTCAACAACTTTAAGGCTTAGACCTGGGAGGTATAATTCCTCTCCCTTGCCTTCCTCTCCTCTTGATTTTCTTAATTTACTAATCAAATGATTACCTTTTACGACCAGTATTTATATAAATCGATGATTAGTATAGATATCAACTAATTATAATCATGGCAATCATTGATTAACATTTGATCTATAATGTGTATTAATTTGTGTTATATAAAAAATTGTTTCTTTAGCTGTTGTTTCTGTAATATATATGAAGGTACGAAATCAAGTTCTTAGTGGATGGAGAATGGCAGCTATCACCAGAATTTCCCACTGTTGGCGAAGGTTTGATGGAAAACAATCTGTTAGTTGTGGAATAGTTATGCTGTCAATGATTCAAAAATATGTAAATCAAACTTATAAAGTGTAACTTCAAGTTCATCTTTAACATCTGCTGTTATATAATCATATAATATCCGGTACAGTACTTGGTAGAAGTATGATCTTTACATTTATGGAAGATCTTACACTTGTACCAACTGCCTTGACTTTGCAGCATCTGATTTTTTCAATCCAATTGCCTTGATTGAGTTAGTATTCTCATA

mRNA sequence

ATGAATGAGCCTGCCAATATGGAGATTTGTGCTCGAAGGACTTGTCTGGACAACCAAGTTTTACTGAGTTCCAAGAATGCAAGAAAGTTAAAGATGGAAAACTTGAATAACCCTTCATGTAATATGGCAACCTGGAGCTTTAGGATGGGTTCTTGCAAGTTGATCCATTCTCATGAACCACCTGTAACCACACTTCCTCAAATGTATCGGATTCACAAAAGATATTCCTTGTCTGTCACATCAGAGGAATCTTCAATGCAATCAGAAGATCTTTCTACAGACGAAGAGGAAATTGTGAGTGCAGAGGAACTTCTGGCTCAACCCCTTAGTAGTGAACAGCTGAATGCATTACTGGCTGATTCAGAAAGAGAGAAGCTTGTCAAAAAGCTAAGTCACGCTAATCAGCAGAACAGACTTCTCAAACGACAGCTACATGTAAAGGATGAAGATTTAGTTAACTGTAAGACCGAACTTGCTGCCTTGGACCATGAAATTCAGAGTGTGCAAGTTATGGGAACTTTTGATGGCTGGAGTGTAGGGGAGGAGTTGTCACCTGAATACACTGGGGCTTACTCTAAGTTCTCAACAACTTTAAGGCTTAGACCTGGGAGGTACGAAATCAAGTTCTTAGTGGATGGAGAATGGCAGCTATCACCAGAATTTCCCACTGTTGGCGAAGGTTTGATGGAAAACAATCTGTTAGTTGTGGAATAGTTATGCTGTCAATGATTCAAAAATATGTAAATCAAACTTATAAAGTGTAACTTCAAGTTCATCTTTAACATCTGCTGTTATATAATCATATAATATCCGGTACAGTACTTGGTAGAAGTATGATCTTTACATTTATGGAAGATCTTACACTTGTACCAACTGCCTTGACTTTGCAGCATCTGATTTTTTCAATCCAATTGCCTTGATTGAGTTAGTATTCTCATA

Coding sequence (CDS)

ATGAATGAGCCTGCCAATATGGAGATTTGTGCTCGAAGGACTTGTCTGGACAACCAAGTTTTACTGAGTTCCAAGAATGCAAGAAAGTTAAAGATGGAAAACTTGAATAACCCTTCATGTAATATGGCAACCTGGAGCTTTAGGATGGGTTCTTGCAAGTTGATCCATTCTCATGAACCACCTGTAACCACACTTCCTCAAATGTATCGGATTCACAAAAGATATTCCTTGTCTGTCACATCAGAGGAATCTTCAATGCAATCAGAAGATCTTTCTACAGACGAAGAGGAAATTGTGAGTGCAGAGGAACTTCTGGCTCAACCCCTTAGTAGTGAACAGCTGAATGCATTACTGGCTGATTCAGAAAGAGAGAAGCTTGTCAAAAAGCTAAGTCACGCTAATCAGCAGAACAGACTTCTCAAACGACAGCTACATGTAAAGGATGAAGATTTAGTTAACTGTAAGACCGAACTTGCTGCCTTGGACCATGAAATTCAGAGTGTGCAAGTTATGGGAACTTTTGATGGCTGGAGTGTAGGGGAGGAGTTGTCACCTGAATACACTGGGGCTTACTCTAAGTTCTCAACAACTTTAAGGCTTAGACCTGGGAGGTACGAAATCAAGTTCTTAGTGGATGGAGAATGGCAGCTATCACCAGAATTTCCCACTGTTGGCGAAGGTTTGATGGAAAACAATCTGTTAGTTGTGGAATAG

Protein sequence

MNEPANMEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLPQMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKLVKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVVE
Homology
BLAST of Lsi05G003300 vs. ExPASy Swiss-Prot
Match: Q94AX2 (Protein PTST, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 9.4e-42
Identity = 105/230 (45.65%), Postives = 132/230 (57.39%), Query Frame = 0

Query: 73  KRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKLVKKLSH 132
           KR+ + V +EESS+ SEDL          +E L++PL+S++L +LL D+ER KLVKKLS 
Sbjct: 58  KRFLVGVGTEESSL-SEDL---------LDESLSRPLTSDELKSLLIDTERSKLVKKLSE 117

Query: 133 ANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------------- 192
           ANQQNR LKRQL  ++ ++ N KTELA ++ E+Q                          
Sbjct: 118 ANQQNRFLKRQLKTQEHEITNIKTELALMELEVQALVKLAEEIANLGIPQGSRKISGKYI 177

Query: 193 ---------------------------------------SVQVMGTFDGWSVGEELSPEY 238
                                                  SVQVMG+FDGWS  E+LSPEY
Sbjct: 178 QSHLLSRLDAVQKKMKEQIKGVEAAQSKEVHVFWIGMAESVQVMGSFDGWSQREDLSPEY 237

BLAST of Lsi05G003300 vs. ExPASy Swiss-Prot
Match: Q9LFY0 (Protein PTST homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST2 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-10
Identity = 52/170 (30.59%), Postives = 84/170 (49.41%), Query Frame = 0

Query: 78  SVTSEESSMQSEDLSTD----EEEIVSAEELLAQ-----PLSSEQLNALLADSER--EKL 137
           S  SE ++   E+LS D    E EI+ A+  L        +   ++   + D++R   + 
Sbjct: 379 SKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLAVLEGKMAMAIIDAQRIVREK 438

Query: 138 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPE 197
            +++ HA++  RLL              +T      +    V + G+FDGWS   ++   
Sbjct: 439 QRRIDHASRALRLL--------------RTASIVWPNSASEVLLTGSFDGWSTQRKMKKA 498

Query: 198 YTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVV 237
             G    FS +L+L PG+YEIKF+VDG+W++ P  P V  G  ENNLL++
Sbjct: 499 ENGV---FSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLII 531

BLAST of Lsi05G003300 vs. ExPASy Swiss-Prot
Match: F4KFB3 (Protein PTST homolog 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.2e-10
Identity = 45/137 (32.85%), Postives = 72/137 (52.55%), Query Frame = 0

Query: 113 QLNALLADSEREKLVKKLSHANQQNRLLKRQLHV--KDEDLVNCKTELAALDHEI----- 172
           +L+ L    E+EKL   +     +  + K Q+ +  K+ +L   +  L+ L   +     
Sbjct: 460 ELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEESLSGLQEVVIEYCG 519

Query: 173 --QSVQVMGTFDGWS--VGEEL-SPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPE 232
              +V+V G+F+GW   VG EL + +  G    +ST L L PG YEIKF+VDG+W   P+
Sbjct: 520 DGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYEIKFIVDGQWITDPQ 579

Query: 233 FPTVGEGLMENNLLVVE 238
             +V  G + NN+L V+
Sbjct: 580 KDSVTRGHISNNILKVD 596

BLAST of Lsi05G003300 vs. ExPASy Swiss-Prot
Match: Q10F03 (Protein FLOURY ENDOSPERM 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FLO6 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-09
Identity = 48/149 (32.21%), Postives = 72/149 (48.32%), Query Frame = 0

Query: 88  SEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKLVKKLSHANQQNRLLKRQLHVK 147
           S+D   +E +++ A+E L        + A +A  E  K+  ++   N+     +R+L   
Sbjct: 390 SDDWEFEETKVMQAQEEL------RSIRAKIAVLE-GKMALEIIEKNKIIEEKQRRLDEA 449

Query: 148 DEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPEYTGAYSKFSTTLRLRPGRYEI 207
           ++ L   +T      +    V + G+FDGW+    +     G    FS  LRL PGRYEI
Sbjct: 450 EKALSELRTVYIVWSNPASEVLLTGSFDGWTSQRRMERSERGT---FSLNLRLYPGRYEI 509

Query: 208 KFLVDGEWQLSPEFPTVGEGLMENNLLVV 237
           KF+VDG W+  P  P V     ENNLL V
Sbjct: 510 KFIVDGVWRNDPLRPLVSNNGHENNLLTV 528

BLAST of Lsi05G003300 vs. ExPASy TrEMBL
Match: A0A5D3BTZ2 (Protein PTST OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001330 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.5e-103
Identity = 213/296 (71.96%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDN VLLSSK ARKLK ENLNNPSCNMATWSFR GSCKLIHSHEP VT  P
Sbjct: 1   MEICAPRTCLDNLVLLSSKKARKLKRENLNNPSCNMATWSFRTGSCKLIHSHEPSVTIRP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
           Q Y+I KRYSL VTSEESSMQSEDLST EEEIVS EELLAQPLSSEQLNALLADSER++L
Sbjct: 61  QSYQILKRYSLPVTSEESSMQSEDLSTHEEEIVSGEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------- 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ                    
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 ---------------------------------------------SVQVMGTFDGWSVGE 238
                                                        +VQVMGTFDGWSVGE
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIVDQIKDVDLVQSKEVPLFWYGMAENVQVMGTFDGWSVGE 240

BLAST of Lsi05G003300 vs. ExPASy TrEMBL
Match: A0A1S3C4P6 (protein PTST, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496819 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.5e-103
Identity = 213/296 (71.96%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDN VLLSSK ARKLK ENLNNPSCNMATWSFR GSCKLIHSHEP VT  P
Sbjct: 1   MEICAPRTCLDNLVLLSSKKARKLKRENLNNPSCNMATWSFRTGSCKLIHSHEPSVTIRP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
           Q Y+I KRYSL VTSEESSMQSEDLST EEEIVS EELLAQPLSSEQLNALLADSER++L
Sbjct: 61  QSYQILKRYSLPVTSEESSMQSEDLSTHEEEIVSGEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------- 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ                    
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 ---------------------------------------------SVQVMGTFDGWSVGE 238
                                                        +VQVMGTFDGWSVGE
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIVDQIKDVDLVQSKEVPLFWYGMAENVQVMGTFDGWSVGE 240

BLAST of Lsi05G003300 vs. ExPASy TrEMBL
Match: A0A6J1CNQ5 (protein PTST, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013285 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.1e-88
Identity = 190/297 (63.97%), Postives = 206/297 (69.36%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLD QV LSS+NA KLK EN N+ SCNMA WSFRM S KL+H + P V   P
Sbjct: 1   MEICAPRTCLDKQVSLSSQNAIKLKRENTNSLSCNMAAWSFRMDSFKLVHFYAPSVMRYP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTD-EEEIVSAEELLAQPLSSEQLNALLADSEREK 126
           Q  R H+RYSL VTSEESSMQSED +TD EEEIVS EELLAQPLSSEQLNALLADSEREK
Sbjct: 61  QNRRSHRRYSLPVTSEESSMQSEDPATDVEEEIVSTEELLAQPLSSEQLNALLADSEREK 120

Query: 127 LVKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ------------------- 186
           LVKKLSHANQQNRLLKRQLHVKD+DLV  KTELAAL+HE Q                   
Sbjct: 121 LVKKLSHANQQNRLLKRQLHVKDDDLVKLKTELAALEHEFQVLIKLAEEIAQSGIPEGSR 180

Query: 187 ----------------------------------------------SVQVMGTFDGWSVG 238
                                                         SVQVMG+FDGWS+G
Sbjct: 181 KINGKYIQSHLLTKLEGVHKKIEDQIKDVHLVQSKEISLFWYGMAESVQVMGSFDGWSIG 240

BLAST of Lsi05G003300 vs. ExPASy TrEMBL
Match: A0A6J1G9H0 (protein PTST, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452163 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.2e-86
Identity = 188/296 (63.51%), Postives = 204/296 (68.92%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEI A R+CL+ QVLLS K ARKLK ENLN+PSCN+ T SFRMGS  LIHS EP V T P
Sbjct: 1   MEIYAPRSCLEKQVLLSFKIARKLKRENLNSPSCNITTRSFRMGSGMLIHSREPSVMTRP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
             Y I +R+SL VTSEESSMQS+DLS D EEI SAEELLA+PLSSEQLNALLA+SERE+L
Sbjct: 61  HSYWIQRRHSLPVTSEESSMQSKDLSLDAEEIESAEELLAEPLSSEQLNALLANSERERL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEI--------------------- 186
           VK+LSHANQQNRLLKRQL V+D DLVN KTELA LDHE+                     
Sbjct: 121 VKRLSHANQQNRLLKRQLRVQDNDLVNFKTELAGLDHEVEGLIKLAEEIAQSGIPEGTRK 180

Query: 187 --------------------------------------------QSVQVMGTFDGWSVGE 238
                                                       +SVQVMGTFDGWSVGE
Sbjct: 181 INGKYIQSHLLTKLKAVHKKIENQIKDVDLVQSKEVQLFWYGMAESVQVMGTFDGWSVGE 240

BLAST of Lsi05G003300 vs. ExPASy TrEMBL
Match: A0A1S3C4K9 (protein PTST, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496819 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.9e-86
Identity = 191/296 (64.53%), Postives = 196/296 (66.22%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDN VLLSSK ARKLK ENLNNPSCNMATWSFR                  
Sbjct: 1   MEICAPRTCLDNLVLLSSKKARKLKRENLNNPSCNMATWSFR------------------ 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
                      +VTSEESSMQSEDLST EEEIVS EELLAQPLSSEQLNALLADSER++L
Sbjct: 61  -----------TVTSEESSMQSEDLSTHEEEIVSGEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------- 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ                    
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 ---------------------------------------------SVQVMGTFDGWSVGE 238
                                                        +VQVMGTFDGWSVGE
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIVDQIKDVDLVQSKEVPLFWYGMAENVQVMGTFDGWSVGE 240

BLAST of Lsi05G003300 vs. NCBI nr
Match: XP_008457048.1 (PREDICTED: protein PTST, chloroplastic isoform X1 [Cucumis melo] >TYK01559.1 protein PTST [Cucumis melo var. makuwa])

HSP 1 Score: 385.6 bits (989), Expect = 3.1e-103
Identity = 213/296 (71.96%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDN VLLSSK ARKLK ENLNNPSCNMATWSFR GSCKLIHSHEP VT  P
Sbjct: 1   MEICAPRTCLDNLVLLSSKKARKLKRENLNNPSCNMATWSFRTGSCKLIHSHEPSVTIRP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
           Q Y+I KRYSL VTSEESSMQSEDLST EEEIVS EELLAQPLSSEQLNALLADSER++L
Sbjct: 61  QSYQILKRYSLPVTSEESSMQSEDLSTHEEEIVSGEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------- 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ                    
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 ---------------------------------------------SVQVMGTFDGWSVGE 238
                                                        +VQVMGTFDGWSVGE
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIVDQIKDVDLVQSKEVPLFWYGMAENVQVMGTFDGWSVGE 240

BLAST of Lsi05G003300 vs. NCBI nr
Match: XP_004145836.1 (protein PTST, chloroplastic isoform X1 [Cucumis sativus] >KAE8650603.1 hypothetical protein Csa_009755 [Cucumis sativus])

HSP 1 Score: 381.7 bits (979), Expect = 4.5e-102
Identity = 211/296 (71.28%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDNQVLLSSKNA KLK E LNNPSCN+ATWSFR GSCKLIHSHEP V T P
Sbjct: 1   MEICAPRTCLDNQVLLSSKNATKLKREKLNNPSCNIATWSFRTGSCKLIHSHEPSVITHP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
           Q Y+I KRYSL VTSEESS QSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSER++L
Sbjct: 61  QTYQILKRYSLPVTSEESSTQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSERDRL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------- 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDH+IQ                    
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHDIQGLIKLAEEIAQSGIPERTRK 180

Query: 187 ---------------------------------------------SVQVMGTFDGWSVGE 238
                                                        +VQVMGTFDGWSVGE
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIMDQIKDVDLVQSKEVPLFWYGMAENVQVMGTFDGWSVGE 240

BLAST of Lsi05G003300 vs. NCBI nr
Match: XP_038906240.1 (protein PTST, chloroplastic isoform X1 [Benincasa hispida] >XP_038906248.1 protein PTST, chloroplastic isoform X1 [Benincasa hispida] >XP_038906257.1 protein PTST, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 381.3 bits (978), Expect = 5.9e-102
Identity = 213/296 (71.96%), Postives = 217/296 (73.31%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLDNQVLL SKNARKLK ENLNNPSCNMA  +FR GSCKLIHSHEP V TLP
Sbjct: 1   MEICAPRTCLDNQVLLGSKNARKLKRENLNNPSCNMAIQTFRTGSCKLIHSHEPSVKTLP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKL 126
           Q Y+I KRYSL VTSEESSMQSE LSTDEE+IVSAEELLAQPLSSEQLNALLADSERE+L
Sbjct: 61  QGYQIIKRYSLPVTSEESSMQSEYLSTDEEDIVSAEELLAQPLSSEQLNALLADSERERL 120

Query: 127 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------- 186
           VKKLSHANQQNRLLKRQLHVKDEDLVNCK E AALDHEIQ                    
Sbjct: 121 VKKLSHANQQNRLLKRQLHVKDEDLVNCKIEFAALDHEIQGLIKLAEEIAQSGIPEGTRK 180

Query: 187 ---------------------------------------------SVQVMGTFDGWSVGE 238
                                                        SVQVMGTFDGWSVGE
Sbjct: 181 INGKYIQSHLLTKLEAVHKKIADQIKDVDLVQSKEVPLFWYGMAESVQVMGTFDGWSVGE 240

BLAST of Lsi05G003300 vs. NCBI nr
Match: XP_022143405.1 (protein PTST, chloroplastic [Momordica charantia])

HSP 1 Score: 336.3 bits (861), Expect = 2.2e-88
Identity = 190/297 (63.97%), Postives = 206/297 (69.36%), Query Frame = 0

Query: 7   MEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEPPVTTLP 66
           MEICA RTCLD QV LSS+NA KLK EN N+ SCNMA WSFRM S KL+H + P V   P
Sbjct: 1   MEICAPRTCLDKQVSLSSQNAIKLKRENTNSLSCNMAAWSFRMDSFKLVHFYAPSVMRYP 60

Query: 67  QMYRIHKRYSLSVTSEESSMQSEDLSTD-EEEIVSAEELLAQPLSSEQLNALLADSEREK 126
           Q  R H+RYSL VTSEESSMQSED +TD EEEIVS EELLAQPLSSEQLNALLADSEREK
Sbjct: 61  QNRRSHRRYSLPVTSEESSMQSEDPATDVEEEIVSTEELLAQPLSSEQLNALLADSEREK 120

Query: 127 LVKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ------------------- 186
           LVKKLSHANQQNRLLKRQLHVKD+DLV  KTELAAL+HE Q                   
Sbjct: 121 LVKKLSHANQQNRLLKRQLHVKDDDLVKLKTELAALEHEFQVLIKLAEEIAQSGIPEGSR 180

Query: 187 ----------------------------------------------SVQVMGTFDGWSVG 238
                                                         SVQVMG+FDGWS+G
Sbjct: 181 KINGKYIQSHLLTKLEGVHKKIEDQIKDVHLVQSKEISLFWYGMAESVQVMGSFDGWSIG 240

BLAST of Lsi05G003300 vs. NCBI nr
Match: KAG6607014.1 (Protein PTST, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 332.4 bits (851), Expect = 3.2e-87
Identity = 189/302 (62.58%), Postives = 207/302 (68.54%), Query Frame = 0

Query: 1   MNEPANMEICARRTCLDNQVLLSSKNARKLKMENLNNPSCNMATWSFRMGSCKLIHSHEP 60
           + EP +MEI A R+CL+ QVLLS K ARKLK ENLN+PSCN+ T SFRMGS  LIHS EP
Sbjct: 100 LEEPTDMEIYAPRSCLEKQVLLSFKIARKLKRENLNSPSCNITTRSFRMGSGMLIHSREP 159

Query: 61  PVTTLPQMYRIHKRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLAD 120
            V T P  Y I +R+SL VTSEESSMQ +DLS D EEI SAEELLA+PLSSEQLNALLA+
Sbjct: 160 SVMTRPHSYWIQRRHSLPVTSEESSMQPKDLSLDAEEIESAEELLAEPLSSEQLNALLAN 219

Query: 121 SEREKLVKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEI--------------- 180
           SERE+LVK+LSHANQQNRLLKRQL V+D DLVN KTELA LDHE+               
Sbjct: 220 SERERLVKRLSHANQQNRLLKRQLRVQDNDLVNFKTELAGLDHEVEGLIKLAEEIAQSGI 279

Query: 181 --------------------------------------------------QSVQVMGTFD 238
                                                             +SVQVMGTFD
Sbjct: 280 PEGTRKINGKYIQSHLLTKLKAVHKKIENQIKDVDLVQSKEVQLFWYGMAESVQVMGTFD 339

BLAST of Lsi05G003300 vs. TAIR 10
Match: AT5G39790.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 166.0 bits (419), Expect = 3.7e-41
Identity = 105/230 (45.65%), Postives = 130/230 (56.52%), Query Frame = 0

Query: 73  KRYSLSVTSEESSMQSEDLSTDEEEIVSAEELLAQPLSSEQLNALLADSEREKLVKKLSH 132
           KR+ + V +EESS+ SEDL          +E L++PL+S++L +LL D+ER KLVKKLS 
Sbjct: 58  KRFLVGVGTEESSL-SEDL---------LDESLSRPLTSDELKSLLIDTERSKLVKKLSE 117

Query: 133 ANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQ-------------------------- 192
           ANQQNR LKRQ H    ++ N KTELA ++ E+Q                          
Sbjct: 118 ANQQNRFLKRQEH----EITNIKTELALMELEVQALVKLAEEIANLGIPQGSRKISGKYI 177

Query: 193 ---------------------------------------SVQVMGTFDGWSVGEELSPEY 238
                                                  SVQVMG+FDGWS  E+LSPEY
Sbjct: 178 QSHLLSRLDAVQKKMKEQIKGVEAAQSKEVHVFWIGMAESVQVMGSFDGWSQREDLSPEY 237

BLAST of Lsi05G003300 vs. TAIR 10
Match: AT1G27070.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 68.2 bits (165), Expect = 1.0e-11
Identity = 52/170 (30.59%), Postives = 84/170 (49.41%), Query Frame = 0

Query: 78  SVTSEESSMQSEDLSTD----EEEIVSAEELLAQ-----PLSSEQLNALLADSER--EKL 137
           S  SE ++   E+LS D    E EI+ A+  L        +   ++   + D++R   + 
Sbjct: 379 SKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLAVLEGKMAMAIIDAQRIVREK 438

Query: 138 VKKLSHANQQNRLLKRQLHVKDEDLVNCKTELAALDHEIQSVQVMGTFDGWSVGEELSPE 197
            +++ HA++  RLL              +T      +    V + G+FDGWS   ++   
Sbjct: 439 QRRIDHASRALRLL--------------RTASIVWPNSASEVLLTGSFDGWSTQRKMKKA 498

Query: 198 YTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEFPTVGEGLMENNLLVV 237
             G    FS +L+L PG+YEIKF+VDG+W++ P  P V  G  ENNLL++
Sbjct: 499 ENGV---FSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLII 531

BLAST of Lsi05G003300 vs. TAIR 10
Match: AT5G03420.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-11
Identity = 45/137 (32.85%), Postives = 72/137 (52.55%), Query Frame = 0

Query: 113 QLNALLADSEREKLVKKLSHANQQNRLLKRQLHV--KDEDLVNCKTELAALDHEI----- 172
           +L+ L    E+EKL   +     +  + K Q+ +  K+ +L   +  L+ L   +     
Sbjct: 460 ELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEESLSGLQEVVIEYCG 519

Query: 173 --QSVQVMGTFDGWS--VGEEL-SPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPE 232
              +V+V G+F+GW   VG EL + +  G    +ST L L PG YEIKF+VDG+W   P+
Sbjct: 520 DGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYEIKFIVDGQWITDPQ 579

Query: 233 FPTVGEGLMENNLLVVE 238
             +V  G + NN+L V+
Sbjct: 580 KDSVTRGHISNNILKVD 596

BLAST of Lsi05G003300 vs. TAIR 10
Match: AT3G01510.1 (like SEX4 1 )

HSP 1 Score: 43.1 bits (100), Expect = 3.6e-04
Identity = 23/75 (30.67%), Postives = 40/75 (53.33%), Query Frame = 0

Query: 163 HEIQSVQVMGTFDG-WSVGEELSPEYTGAYSKFSTTLRLRPGRYEIKFLVDGEWQLSPEF 222
           HE + V ++G F G W   E +   + G   +F T +RL  G+Y  K++++G+W+ S   
Sbjct: 464 HEGEEVLLVGDFTGNWK--EPIKATHKGG-PRFETEVRLTQGKYYYKYIINGDWRHSATS 523

Query: 223 PTVGEGLMENNLLVV 237
           PT  +     N ++V
Sbjct: 524 PTERDDRGNTNNIIV 535

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AX29.4e-4245.65Protein PTST, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1[more]
Q9LFY01.5e-1030.59Protein PTST homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST2 P... [more]
F4KFB33.2e-1032.85Protein PTST homolog 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE... [more]
Q10F031.6e-0932.21Protein FLOURY ENDOSPERM 6, chloroplastic OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A5D3BTZ21.5e-10371.96Protein PTST OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001330 ... [more]
A0A1S3C4P61.5e-10371.96protein PTST, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496819 P... [more]
A0A6J1CNQ51.1e-8863.97protein PTST, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013285 PE=4 ... [more]
A0A6J1G9H02.2e-8663.51protein PTST, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452163 PE=4 S... [more]
A0A1S3C4K94.9e-8664.53protein PTST, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496819 P... [more]
Match NameE-valueIdentityDescription
XP_008457048.13.1e-10371.96PREDICTED: protein PTST, chloroplastic isoform X1 [Cucumis melo] >TYK01559.1 pro... [more]
XP_004145836.14.5e-10271.28protein PTST, chloroplastic isoform X1 [Cucumis sativus] >KAE8650603.1 hypotheti... [more]
XP_038906240.15.9e-10271.96protein PTST, chloroplastic isoform X1 [Benincasa hispida] >XP_038906248.1 prote... [more]
XP_022143405.12.2e-8863.97protein PTST, chloroplastic [Momordica charantia][more]
KAG6607014.13.2e-8762.58Protein PTST, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G39790.13.7e-4145.655'-AMP-activated protein kinase-related [more]
AT1G27070.11.0e-1130.595'-AMP-activated protein kinase-related [more]
AT5G03420.12.3e-1132.855'-AMP-activated protein kinase-related [more]
AT3G01510.13.6e-0430.67like SEX4 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 123..143
NoneNo IPR availablePANTHERPTHR47342PROTEIN PTST, CHLOROPLASTICcoord: 166..237
coord: 10..167
NoneNo IPR availableCDDcd02859E_set_AMPKbeta_like_Ncoord: 164..236
e-value: 2.61514E-23
score: 87.6544
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 147..236
e-value: 1.2E-17
score: 65.5
IPR032640AMP-activated protein kinase, glycogen-binding domainPFAMPF16561AMPK1_CBMcoord: 164..226
e-value: 9.4E-14
score: 51.4
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 161..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G003300.1Lsi05G003300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009507 chloroplast
molecular_function GO:0030247 polysaccharide binding