Lsi05G003230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G003230
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionmajor latex protein 149-like
Locationchr05: 4107354 .. 4110163 (+)
RNA-Seq ExpressionLsi05G003230
SyntenyLsi05G003230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCTTGAAAATGGCTCAGATTGCTAAGATCTCAGAGCAATTGCAGCTCAAGTCTTCTGCTCACAAGTTCTATGAATTTTTCAAGAACAAAATGGACTGTTTCATTCAAATGTTCCCTCACATTTTTACAAGCTACAAAATTGTCGAAGGAAATGGTTTTTCTCATGGCAGCGTCATCCACGTAAAATATAACTTAGGTGAGGATATTTTATTTACCATCATTTTTCCAACTTTTATTTTATTTTGGTTCGTAAACTTTCAAATACTCTATTTTCATGTTTCAACTTTGAACGTGATTTAATTTTAAGCCCTTATACATATTCACTACACTCGCTATTATTTGTATGTATGTATGATCAAAGTTTAGGATATCTTGCTTCTTCTTTTTTCGAATTCAACAAATATGCGTGAGAATCAAGCTTTCAATCTTACACGAATGTATAAATGTCTTAACCTGTAGAATTATATACACATGATGGTGAATCTAATACTACATATTGAGAGAATGAAATTGTTTATATCTACACATTTAAATAAAATATATTTTTGGTTCAATTACATAAATTATTCAAATAATTATTATCCAAAATATATAGCAAAATTTTCACAAATAGAAAAAATGTCAAAGCATTTACATAAAATAGCAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACTGATAGACATTGATAGACTTCTATCAGCATCTATTAGTGATAGACTTCTATTATTTCTATCACTGATAGACCTTGATAGACTTCTATCAGCGTCTATCCCAACTATCTAAAAATTTTGCTATTTTGTGTAAATAATTTTCCTTATTTTTCCATTTTTTAAATTTGCCCAAATATATATGTACCAATAGCTTTCTTTATTCAAGCTTTTAAGTTCCTTAATAGATTTAGAGTCCTCTTTAGAATACCTTCATATTTTAAGCAAAATTTCTTAAATGTTTTAAATAAAATTTTGTTTATTTCTTACATTATTTTAAGATCATCACATTTTAATTACAAGTATATTTTGTTTCTTACTATATCTTTGAAAACAAATCAGATAATCACATAAAAGTCATTAAGTGTTTTCAAAATTAGCTTTTAAATTAATTCAAAAATTTATAAGTGCTTATTTATTGAGATTAAGAAAAAAACACTATTGACTATCTAAAATAATTATATTATATTTAAATTATAAAAATTGATTTTTGTCTAGAAAGCATTTTTGTTCAAAATTATTCCAAACAAACCACTAACACTATATGATACAACAAAAAACAGATTATAACTTGAGATCGTTTTCTCCGCCCCTCGTATTTAATCTAATACCTTTGCCAAATAAAAAAAAAAAACTTACACGACATCATATTATTCTTGTTTATATATATATATATATATATATATATATATTTGAGTTCAACCTTAGGTGTGAGATTTGGTAGGAAATTTGAAAACATAATTTTAAAATGAAGAGATACAATGAAAATATAAATGTATTTCATGCCTTCAATATGTGTTTAGTATAATGGATTCAAAAATTAAATTCCAATTGAAACAAATTTTCAAAATATGTTTGATAATATATCTATAAACCATAAAACTAATTATAATTACTCACTAAATATTAGGTTGAATATAAACTATTATTGATAATATATGAACTTGTTTTATGTTAAAAATTTTGTATAATTTTAATTTAACAAAAATAATTGAGTTTATAGCATGAAGTACTATTATTGTTGTTGTTTTTATCTTTTCTATTATAATTCTCTACATTTCAAAATTTGAAATTGAAATCTGGATACACAAAAGAGATAAAAATATCGTTTTCAGAATTTGCACTGTTTGGATTATAAAATGCAAAAACAATTTTCGAAAACAGAATTCGAACGGCCTACCAAATGCAGATTTGTCGAACTCAATGAAACTGAAAACATAAAGTAGAATCTAAATTGCCTATATTTGCAAATTAGAATATTTTGTATAAATATAGCAAAATGGCAATAATTTCATATATAGATTATACTTTGTATGAATTGGCCATTTAGATATACTTGTTATATCAATTTTCATATATAGATTGTTATAGAAAAATTATAAAAGATATTTTCATATATAGCAAAATGGCCAACTTATTTACAAATATAACAAAATAGTAAAAAGTTTCCTCATATGAAAACATACTATTTTGCTATATTTGAAAATATTTCCACAAATTTGTCATTTAAAACAACTTCTCTAATAAAAAAAATGAAAAATGAGATTTAAATTTTTTTCCCTATATTTCCAATTTTTAAAAACCATTGCTCTAGCTTTGCTTATACCTTTTTTTTTCTCTCTCTCTCTCTTGTTACCTATTACAATTTCCCTATTACTTATTATATATTGTATACACACCCTTTACAACTTACTATCCATCCAAGCTTTTTGGACTTCAATTCTTACCCTCTTTTTCTTGTTGTATGGAAATTACATAGGTAGCCCAGCAGAGGCAAAGGAGAGGCTAGCCATTGATGATGCTAACAAATCAATAACTTTTGAATTCCTTGAGGGAGATCTATTCAAAGATTTTGAAGTGTTCAAAATGAAAATTCAAGTTTTTGAGAATGGTAGCAATGGAAGCTCAGTTAATTGGTCTATAGAATTTGTGAAGGCAAATGAAGATGTGGCTCCACCACAAAACTATCTCCTTTTTGCAGCTAAAACCAGCAAAAGCCTTGATGATTACCTTTGCAACAAC

mRNA sequence

AAGCTTGAAAATGGCTCAGATTGCTAAGATCTCAGAGCAATTGCAGCTCAAGTCTTCTGCTCACAAGTTCTATGAATTTTTCAAGAACAAAATGGACTGTTTCATTCAAATGTTCCCTCACATTTTTACAAGCTACAAAATTGTCGAAGGAAATGGTAGCCCAGCAGAGGCAAAGGAGAGGCTAGCCATTGATGATGCTAACAAATCAATAACTTTTGAATTCCTTGAGGGAGATCTATTCAAAGATTTTGAAGTGTTCAAAATGAAAATTCAAGTTTTTGAGAATGGTAGCAATGGAAGCTCAGTTAATTGGTCTATAGAATTTGTGAAGGCAAATGAAGATGTGGCTCCACCACAAAACTATCTCCTTTTTGCAGCTAAAACCAGCAAAAGCCTTGATGATTACCTTTGCAACAAC

Coding sequence (CDS)

ATGGCTCAGATTGCTAAGATCTCAGAGCAATTGCAGCTCAAGTCTTCTGCTCACAAGTTCTATGAATTTTTCAAGAACAAAATGGACTGTTTCATTCAAATGTTCCCTCACATTTTTACAAGCTACAAAATTGTCGAAGGAAATGGTAGCCCAGCAGAGGCAAAGGAGAGGCTAGCCATTGATGATGCTAACAAATCAATAACTTTTGAATTCCTTGAGGGAGATCTATTCAAAGATTTTGAAGTGTTCAAAATGAAAATTCAAGTTTTTGAGAATGGTAGCAATGGAAGCTCAGTTAATTGGTCTATAGAATTTGTGAAGGCAAATGAAGATGTGGCTCCACCACAAAACTATCTCCTTTTTGCAGCTAAAACCAGCAAAAGCCTTGATGATTACCTTTGCAACAAC

Protein sequence

MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGNGSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVFENGSNGSSVNWSIEFVKANEDVAPPQNYLLFAAKTSKSLDDYLCNN
Homology
BLAST of Lsi05G003230 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.1e-14
Identity = 49/144 (34.03%), Postives = 77/144 (53.47%), Query Frame = 0

Query: 7   ISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------NGSP 66
           +  ++++K+SA KF+  F  K     +  P    S  + EG               +G  
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  AEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFVKAN 126
             AKER+ A+D     ITF  +EGDL K+++ F + IQV  ++G +GS V+W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVAPPQNYLLFAAKTSKSLDDYL 134
           E+VA P+  L FA + SK +D++L
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312

BLAST of Lsi05G003230 vs. ExPASy Swiss-Prot
Match: Q9SSK9 (MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.5e-12
Identity = 46/147 (31.29%), Postives = 75/147 (51.02%), Query Frame = 0

Query: 4   IAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------N 63
           + K+   +++K+SA KF+  F  K     +  P       + EG               +
Sbjct: 23  VGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYVHD 82

Query: 64  GSPAEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFV 123
           G    AKER+ A++     ITF  +EGDL K+++ F + IQV  + G  GS V+W +E+ 
Sbjct: 83  GEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLEYE 142

Query: 124 KANEDVAPPQNYLLFAAKTSKSLDDYL 134
           K +E+VA P+  L F  + SK +D++L
Sbjct: 143 KISEEVAHPETLLQFCVEVSKEIDEHL 169

BLAST of Lsi05G003230 vs. ExPASy Swiss-Prot
Match: Q941R6 (MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 1.5e-12
Identity = 45/147 (30.61%), Postives = 76/147 (51.70%), Query Frame = 0

Query: 6   KISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------NGS 65
           K+   +++K+SA KF+  F  +     +  P      ++ EG               +G 
Sbjct: 23  KLETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYVHDGE 82

Query: 66  PAEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFVKA 125
              AKER+ A++     ITF  +EGDL K+++ F + IQV  + G  GS V+W +E+ K 
Sbjct: 83  AKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYEKI 142

Query: 126 NEDVAPPQNYLLFAAKTSKSLDDYLCN 136
           ++ VA P+ +L F  + SK +D++L N
Sbjct: 143 DDKVAHPETFLDFCVEVSKEIDEHLLN 169

BLAST of Lsi05G003230 vs. ExPASy Swiss-Prot
Match: P19825 (Major latex protein 15 OS=Papaver somniferum OX=3469 GN=MLP15 PE=2 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 3.4e-12
Identity = 42/147 (28.57%), Postives = 72/147 (48.98%), Query Frame = 0

Query: 4   IAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGNGS------------- 63
           + K+  + ++  +A K+Y+ FK+  D      PHI+TS K VEG+G+             
Sbjct: 11  VGKLITESEVNCNADKYYQIFKHHED-LPSAIPHIYTSVKAVEGHGTTSGCVKEWCYILE 70

Query: 64  --PAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVFENGS-NGSSVNWSIEFVK 123
             P   KE+   +D  ++I    +EG +  D++ F   + V    +  GS V W +++ K
Sbjct: 71  GKPLTVKEKTTYNDETRTINHNGIEGGMMNDYKKFVATLVVKPKANGQGSIVTWIVDYEK 130

Query: 124 ANEDVAPPQNYLLFAAKTSKSLDDYLC 135
            NED   P +YL F  +  + L+ +LC
Sbjct: 131 INEDSPVPFDYLAFFQQNIEDLNSHLC 156

BLAST of Lsi05G003230 vs. ExPASy Swiss-Prot
Match: Q06394 (Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.2e-11
Identity = 41/147 (27.89%), Postives = 73/147 (49.66%), Query Frame = 0

Query: 4   IAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGNGS------------- 63
           + K+  + ++  +A K+Y+ +K+  D    + PHI+TS K VEG+G+             
Sbjct: 11  VGKLVMESEVNCNADKYYKLYKHHED-LPSVIPHIYTSVKAVEGHGTTSGCVKEWGYILE 70

Query: 64  --PAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFVK 123
             P   KE+   +D  ++I    + GDL  D++ F   + V  ++  +G  V W+I++ K
Sbjct: 71  GKPLSCKEKTTYNDETRTIHHMVVAGDLMNDYKKFDATLVVNPKSNGHGCIVKWTIDYEK 130

Query: 124 ANEDVAPPQNYLLFAAKTSKSLDDYLC 135
            NED   P  YL    + ++ L  +LC
Sbjct: 131 MNEDSPVPFGYLACYQQITEDLSSHLC 156

BLAST of Lsi05G003230 vs. ExPASy TrEMBL
Match: A0A0A0L9D7 (Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G219190 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.0e-51
Identity = 112/151 (74.17%), Postives = 118/151 (78.15%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGN------------ 60
           MAQIAKISEQLQLKSSAHKFY+FF NKMD  IQMFPHIFTSYKI+EGN            
Sbjct: 1   MAQIAKISEQLQLKSSAHKFYDFFTNKMDSLIQMFPHIFTSYKILEGNGFAHASVIHIKY 60

Query: 61  --GSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQV-FENGSNGSSVNWSIEF 120
             G PAE KERLA DDANKSI FE  EGDL +DFEVFKMK+QV  E GSNGS VNWSIEF
Sbjct: 61  NIGGPAEMKERLAFDDANKSIAFEVFEGDLLRDFEVFKMKMQVNNEKGSNGSLVNWSIEF 120

Query: 121 VKANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           VKANEDVA P  YL  AA+TSK+LDDYLCNN
Sbjct: 121 VKANEDVAAPHQYLTIAAQTSKTLDDYLCNN 151

BLAST of Lsi05G003230 vs. ExPASy TrEMBL
Match: A0A0A0LA22 (Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G331340 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.1e-50
Identity = 109/151 (72.19%), Postives = 117/151 (77.48%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGN------------ 60
           MAQIAKISEQ+QLK S HKFY+FF NKMD  IQMFPHI TSYKI+EGN            
Sbjct: 15  MAQIAKISEQVQLKCSGHKFYDFFTNKMDSVIQMFPHIVTSYKILEGNGFAHGSVIHLKY 74

Query: 61  --GSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEF 120
             G PAE KERLA DDANKSI FE  EGDLF+DFEVFKMK+QV  E GSNGSSVNWSIEF
Sbjct: 75  NIGGPAEIKERLAFDDANKSIAFEVFEGDLFRDFEVFKMKMQVINEKGSNGSSVNWSIEF 134

Query: 121 VKANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           VK NEDVA P +YL  AA+TSK+LDDYLCNN
Sbjct: 135 VKENEDVAAPHHYLTIAAQTSKTLDDYLCNN 165

BLAST of Lsi05G003230 vs. ExPASy TrEMBL
Match: A0A6J1F532 (kirola-like OS=Cucurbita moschata OX=3662 GN=LOC111442243 PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.6e-49
Identity = 100/150 (66.67%), Postives = 118/150 (78.67%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGNG----------- 60
           MAQIAK+S+++QL+SS HKFYE  KNKMD   QMFP ++ S+K++EGNG           
Sbjct: 1   MAQIAKVSQKVQLRSSGHKFYELLKNKMDFVFQMFPDVYKSWKVLEGNGLAHGSIIHLKY 60

Query: 61  ---SPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVFENGSNGSSVNWSIEFV 120
               P++AKERLAIDDANKSITFE LEGDLF+DFEVFK+KI+V ENGSNG S NWSIE+V
Sbjct: 61  NVDGPSKAKERLAIDDANKSITFECLEGDLFRDFEVFKLKIEVVENGSNGCSTNWSIEYV 120

Query: 121 KANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           KANEDVAPP NYL+ AAK SK +DDYLC N
Sbjct: 121 KANEDVAPPHNYLIIAAKISKGIDDYLCKN 150

BLAST of Lsi05G003230 vs. ExPASy TrEMBL
Match: A0A6J1IXD7 (kirola-like OS=Cucurbita maxima OX=3661 GN=LOC111481516 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.0e-48
Identity = 98/150 (65.33%), Postives = 116/150 (77.33%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGNG----------- 60
           MAQIAK+S ++QL+SS HKFYEF KNKMD   QMFP I+ S+K+VEGNG           
Sbjct: 1   MAQIAKVSHEVQLRSSGHKFYEFLKNKMDFVFQMFPEIYKSWKVVEGNGYAHGSVIYLKY 60

Query: 61  ---SPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVFENGSNGSSVNWSIEFV 120
               P+E  ERL++DDANKS+TFE++EGDL +DFEVF+MKI+V ENGSNGSS NWSIE+V
Sbjct: 61  IVDGPSEVIERLSMDDANKSLTFEWVEGDLLRDFEVFQMKIEVVENGSNGSSANWSIEYV 120

Query: 121 KANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           KANEDVAPP NYLL  AK SK +DDYLC N
Sbjct: 121 KANEDVAPPHNYLLCVAKVSKGVDDYLCKN 150

BLAST of Lsi05G003230 vs. ExPASy TrEMBL
Match: A0A6J1J5M9 (major latex protein 146-like OS=Cucurbita maxima OX=3661 GN=LOC111481517 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 8.9e-48
Identity = 97/150 (64.67%), Postives = 115/150 (76.67%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGN------------ 60
           MAQIAKIS+Q+QLK   HKFYEFFKNKM+C  QMFP I  S+K++EGN            
Sbjct: 1   MAQIAKISQQVQLKCCGHKFYEFFKNKMECVFQMFPEICCSWKVLEGNEFSHVRVIHLKY 60

Query: 61  --GSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVFENGSNGSSVNWSIEFV 120
             GSP E K ++ +DDANKSITFE +EGD+ +DFEVF+MKI+V ENGSNGSS NWS+E+V
Sbjct: 61  VVGSPKEGKTKVTVDDANKSITFECVEGDVLRDFEVFQMKIEVVENGSNGSSANWSVEYV 120

Query: 121 KANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           KANEDVAPP NYLL  AK SK LD+YLCN+
Sbjct: 121 KANEDVAPPYNYLLCGAKVSKGLDEYLCNS 150

BLAST of Lsi05G003230 vs. NCBI nr
Match: XP_011651277.1 (major latex protein 146 [Cucumis sativus])

HSP 1 Score: 212.6 bits (540), Expect = 2.1e-51
Identity = 112/151 (74.17%), Postives = 118/151 (78.15%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGN------------ 60
           MAQIAKISEQLQLKSSAHKFY+FF NKMD  IQMFPHIFTSYKI+EGN            
Sbjct: 1   MAQIAKISEQLQLKSSAHKFYDFFTNKMDSLIQMFPHIFTSYKILEGNGFAHASVIHIKY 60

Query: 61  --GSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQV-FENGSNGSSVNWSIEF 120
             G PAE KERLA DDANKSI FE  EGDL +DFEVFKMK+QV  E GSNGS VNWSIEF
Sbjct: 61  NIGGPAEMKERLAFDDANKSIAFEVFEGDLLRDFEVFKMKMQVNNEKGSNGSLVNWSIEF 120

Query: 121 VKANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           VKANEDVA P  YL  AA+TSK+LDDYLCNN
Sbjct: 121 VKANEDVAAPHQYLTIAAQTSKTLDDYLCNN 151

BLAST of Lsi05G003230 vs. NCBI nr
Match: KAE8650529.1 (hypothetical protein Csa_011252 [Cucumis sativus])

HSP 1 Score: 212.6 bits (540), Expect = 2.1e-51
Identity = 112/151 (74.17%), Postives = 118/151 (78.15%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGN------------ 60
           MAQIAKISEQLQLKSSAHKFY+FF NKMD  IQMFPHIFTSYKI+EGN            
Sbjct: 15  MAQIAKISEQLQLKSSAHKFYDFFTNKMDSLIQMFPHIFTSYKILEGNGFAHASVIHIKY 74

Query: 61  --GSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQV-FENGSNGSSVNWSIEF 120
             G PAE KERLA DDANKSI FE  EGDL +DFEVFKMK+QV  E GSNGS VNWSIEF
Sbjct: 75  NIGGPAEMKERLAFDDANKSIAFEVFEGDLLRDFEVFKMKMQVNNEKGSNGSLVNWSIEF 134

Query: 121 VKANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           VKANEDVA P  YL  AA+TSK+LDDYLCNN
Sbjct: 135 VKANEDVAAPHQYLTIAAQTSKTLDDYLCNN 165

BLAST of Lsi05G003230 vs. NCBI nr
Match: XP_038893753.1 (kirola-like [Benincasa hispida])

HSP 1 Score: 208.8 bits (530), Expect = 3.0e-50
Identity = 107/151 (70.86%), Postives = 119/151 (78.81%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMD-CFIQMFPHIFTSYKIVEGN----------- 60
           MAQIAKIS+Q+QLKSS HKFYEFFKN MD  F+QMFP I+ S K+VEGN           
Sbjct: 1   MAQIAKISKQVQLKSSGHKFYEFFKNNMDYIFVQMFPEIYQSGKVVEGNGFSHGSVIHLK 60

Query: 61  ---GSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVFENGSNGSSVNWSIEF 120
              G P E KER+AIDDANKSITFE LEGDLF+DFE+ K+KIQVFENGSNGSSVNWS+EF
Sbjct: 61  YNAGKPEEVKERIAIDDANKSITFECLEGDLFRDFELMKLKIQVFENGSNGSSVNWSVEF 120

Query: 121 VKANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           VKANEDVA P +YLL  +K SK LDDYLCNN
Sbjct: 121 VKANEDVATPHHYLLCLSKVSKGLDDYLCNN 151

BLAST of Lsi05G003230 vs. NCBI nr
Match: XP_004151203.3 (major latex protein 149 [Cucumis sativus] >KAE8650619.1 hypothetical protein Csa_011700 [Cucumis sativus])

HSP 1 Score: 207.6 bits (527), Expect = 6.7e-50
Identity = 108/151 (71.52%), Postives = 117/151 (77.48%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGN------------ 60
           MAQIAKISEQ+QLK S HKFY+FF NKMD  IQMFPHI TSYKI+EGN            
Sbjct: 15  MAQIAKISEQVQLKCSGHKFYDFFTNKMDSVIQMFPHIVTSYKILEGNGFAHGSVIHLKY 74

Query: 61  --GSPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEF 120
             G PAE KERLA DDANKSI FE  EGDLF+DFEVFKMK+QV  E GSNGSSVNWSIEF
Sbjct: 75  NIGGPAEIKERLAFDDANKSIAFEVFEGDLFRDFEVFKMKMQVINEKGSNGSSVNWSIEF 134

Query: 121 VKANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           VK NEDVA P +YL  AA++SK+LDDYLCNN
Sbjct: 135 VKENEDVAAPHHYLTIAAQSSKTLDDYLCNN 165

BLAST of Lsi05G003230 vs. NCBI nr
Match: KAG6581204.1 (hypothetical protein SDJN03_21206, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017943.1 hypothetical protein SDJN02_19809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 206.8 bits (525), Expect = 1.2e-49
Identity = 101/150 (67.33%), Postives = 118/150 (78.67%), Query Frame = 0

Query: 1   MAQIAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEGNG----------- 60
           MAQIAK+S+++QL+SS HKFYE  KNKMD   QMFP ++ S+K++EGNG           
Sbjct: 1   MAQIAKVSQKVQLRSSGHKFYELLKNKMDFVFQMFPDVYKSWKVLEGNGLAHGSIIHLKY 60

Query: 61  ---SPAEAKERLAIDDANKSITFEFLEGDLFKDFEVFKMKIQVFENGSNGSSVNWSIEFV 120
               P+EAKERLAIDDANKSITFE LEGDLF+DFEVFK+KI+V ENGSNG S NWSIE+V
Sbjct: 61  NVDGPSEAKERLAIDDANKSITFECLEGDLFRDFEVFKLKIEVVENGSNGCSTNWSIEYV 120

Query: 121 KANEDVAPPQNYLLFAAKTSKSLDDYLCNN 137
           KANEDVAPP NYL+ AAK SK +DDYLC N
Sbjct: 121 KANEDVAPPHNYLIIAAKISKGIDDYLCKN 150

BLAST of Lsi05G003230 vs. TAIR 10
Match: AT1G70850.1 (MLP-like protein 34 )

HSP 1 Score: 80.1 bits (196), Expect = 1.5e-15
Identity = 49/144 (34.03%), Postives = 77/144 (53.47%), Query Frame = 0

Query: 7   ISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------NGSP 66
           +  ++++K+SA KF+  F  K     +  P    S  + EG               +G  
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  AEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFVKAN 126
             AKER+ A+D     ITF  +EGDL K+++ F + IQV  ++G +GS V+W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVAPPQNYLLFAAKTSKSLDDYL 134
           E+VA P+  L FA + SK +D++L
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312

BLAST of Lsi05G003230 vs. TAIR 10
Match: AT1G70850.3 (MLP-like protein 34 )

HSP 1 Score: 80.1 bits (196), Expect = 1.5e-15
Identity = 49/144 (34.03%), Postives = 77/144 (53.47%), Query Frame = 0

Query: 7   ISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------NGSP 66
           +  ++++K+SA KF+  F  K     +  P    S  + EG               +G  
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  AEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFVKAN 126
             AKER+ A+D     ITF  +EGDL K+++ F + IQV  ++G +GS V+W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVAPPQNYLLFAAKTSKSLDDYL 134
           E+VA P+  L FA + SK +D++L
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312

BLAST of Lsi05G003230 vs. TAIR 10
Match: AT1G70830.2 (MLP-like protein 28 )

HSP 1 Score: 73.9 bits (180), Expect = 1.1e-13
Identity = 46/147 (31.29%), Postives = 75/147 (51.02%), Query Frame = 0

Query: 4   IAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------N 63
           + K+   +++K+SA KF+  F  K     +  P       + EG               +
Sbjct: 23  VGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYVHD 82

Query: 64  GSPAEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFV 123
           G    AKER+ A++     ITF  +EGDL K+++ F + IQV  + G  GS V+W +E+ 
Sbjct: 83  GEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLEYE 142

Query: 124 KANEDVAPPQNYLLFAAKTSKSLDDYL 134
           K +E+VA P+  L F  + SK +D++L
Sbjct: 143 KISEEVAHPETLLQFCVEVSKEIDEHL 169

BLAST of Lsi05G003230 vs. TAIR 10
Match: AT1G70840.1 (MLP-like protein 31 )

HSP 1 Score: 73.9 bits (180), Expect = 1.1e-13
Identity = 45/147 (30.61%), Postives = 76/147 (51.70%), Query Frame = 0

Query: 6   KISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------NGS 65
           K+   +++K+SA KF+  F  +     +  P      ++ EG               +G 
Sbjct: 23  KLETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYVHDGE 82

Query: 66  PAEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFVKA 125
              AKER+ A++     ITF  +EGDL K+++ F + IQV  + G  GS V+W +E+ K 
Sbjct: 83  AKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYEKI 142

Query: 126 NEDVAPPQNYLLFAAKTSKSLDDYLCN 136
           ++ VA P+ +L F  + SK +D++L N
Sbjct: 143 DDKVAHPETFLDFCVEVSKEIDEHLLN 169

BLAST of Lsi05G003230 vs. TAIR 10
Match: AT1G70830.1 (MLP-like protein 28 )

HSP 1 Score: 73.9 bits (180), Expect = 1.1e-13
Identity = 46/147 (31.29%), Postives = 75/147 (51.02%), Query Frame = 0

Query: 4   IAKISEQLQLKSSAHKFYEFFKNKMDCFIQMFPHIFTSYKIVEG---------------N 63
           + K+   +++K+SA KF+  F  K     +  P       + EG               +
Sbjct: 23  VGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYVHD 82

Query: 64  GSPAEAKERL-AIDDANKSITFEFLEGDLFKDFEVFKMKIQVF-ENGSNGSSVNWSIEFV 123
           G    AKER+ A++     ITF  +EGDL K+++ F + IQV  + G  GS V+W +E+ 
Sbjct: 83  GEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLEYE 142

Query: 124 KANEDVAPPQNYLLFAAKTSKSLDDYL 134
           K +E+VA P+  L F  + SK +D++L
Sbjct: 143 KISEEVAHPETLLQFCVEVSKEIDEHL 169

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SSK72.1e-1434.03MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1[more]
Q9SSK91.5e-1231.29MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1[more]
Q941R61.5e-1230.61MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2[more]
P198253.4e-1228.57Major latex protein 15 OS=Papaver somniferum OX=3469 GN=MLP15 PE=2 SV=2[more]
Q063942.2e-1127.89Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L9D71.0e-5174.17Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G219190 PE=... [more]
A0A0A0LA221.1e-5072.19Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G331340 PE=... [more]
A0A6J1F5321.6e-4966.67kirola-like OS=Cucurbita moschata OX=3662 GN=LOC111442243 PE=3 SV=1[more]
A0A6J1IXD74.0e-4865.33kirola-like OS=Cucurbita maxima OX=3661 GN=LOC111481516 PE=3 SV=1[more]
A0A6J1J5M98.9e-4864.67major latex protein 146-like OS=Cucurbita maxima OX=3661 GN=LOC111481517 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_011651277.12.1e-5174.17major latex protein 146 [Cucumis sativus][more]
KAE8650529.12.1e-5174.17hypothetical protein Csa_011252 [Cucumis sativus][more]
XP_038893753.13.0e-5070.86kirola-like [Benincasa hispida][more]
XP_004151203.36.7e-5071.52major latex protein 149 [Cucumis sativus] >KAE8650619.1 hypothetical protein Csa... [more]
KAG6581204.11.2e-4967.33hypothetical protein SDJN03_21206, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT1G70850.11.5e-1534.03MLP-like protein 34 [more]
AT1G70850.31.5e-1534.03MLP-like protein 34 [more]
AT1G70830.21.1e-1331.29MLP-like protein 28 [more]
AT1G70840.11.1e-1330.61MLP-like protein 31 [more]
AT1G70830.11.1e-1331.29MLP-like protein 28 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000916Bet v I/Major latex proteinSMARTSM01037Bet_v_1_2coord: 2..136
e-value: 7.3E-21
score: 85.3
IPR000916Bet v I/Major latex proteinPFAMPF00407Bet_v_1coord: 5..135
e-value: 2.0E-21
score: 76.5
IPR023393START-like domain superfamilyGENE3D3.30.530.20coord: 1..134
e-value: 6.2E-31
score: 109.4
NoneNo IPR availablePANTHERPTHR31907MLP-LIKE PROTEIN 423coord: 1..132
NoneNo IPR availablePANTHERPTHR31907:SF52MAJOR LATEX PROTEINcoord: 1..132
NoneNo IPR availableCDDcd07816Bet_v1-likecoord: 6..133
e-value: 1.07229E-27
score: 98.0304
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 1..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G003230.1Lsi05G003230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response