Lsi05G003120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G003120
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calcium-binding EF-hand family protein
Location	chr05: 3993198 .. 3994363 (+)
RNA-Seq Expression	Lsi05G003120
Synteny	Lsi05G003120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATGGATGAAGAACACATCCCCAATGGCTTCGCAAAATCACAAATCTGATTGCCCTAACTTCAGCAAAAATCAGTTGAACAGAGCGATTTCGATGTAGAAGGAGACAACACCAGGTGTTTCCAATGCCAATAATTTAGGGCAAAATAGCGATTATTAAAGGAGCAATGAAAATTGAAGAAGAGGAGAAAGAAAGAAGCATTTGCCCTAAGTTTCAGATTCATTCCAAATCAAAGTTAAGTGCAGAAAATGGCGGACAGTGGCGTAAGCTCAGCCGAATGCGAGAGGATTTTCAAGCGATTCGATGCTAATGGCGACGGGAAGATCTCGGCGACGGAGCTGGGCGATGCTTTGAACGGATTTGGAGTGAACGCTGAGGACGCCAAGAGGATGATGGACGCCATTGATAAAGATGGCGATGGCTGCATTTCCTTTGAAGAGTTCGCCGATTTTGCTAAGGATAATCGCGCTTTGATGAAGGATTTTGCCAAGGCGTTTTAGGTTTTTGTTTCAATTTAAGATCTGATATATGATGAAGAAAATATGGATATGGTTACAATGAAGGAGATTTTTTTTTTTTTTTACTACGTATATTTAAACATAACATACTATGTTTCTCCTCAATGAATGTTACAAATATTAACTTTGATTAAATCTCATTTTCGTCCCTAAACTTTATTCATAAGCTCTAATTTGAACTTTAGAAAAATGTTTTTTTTGTCCTTCACTTTTAAAATATTTATTTTGATCCTCAAACTTTGATTATTTTGTTCTTACCTTGTGTCTTACTATCTTTAAGATTGATAATTTAAATAGGATCCAACTAAAAAAGACTAGTGATTGGCTCTTAGTATCATTATTATTATTTTCATTTTTTTTTCTAATTTGCTTTTACCTCCCGTGAATATATGCTAATTATTGTTAAGTTATATTTACTTTGGCTTTTTCGAGCTTCGTAGTTCTCCATTGCCTTGCCACTTGTTCTCGATGGTGCCCTCACTGGTAGTCTCCGTTTCAGAAAAAGTGGCATCGGCTTGAGAGTGGTTGCCTCTAACTTATCTATGGAAAGGTTGCTTGGCTTGAGAGTGGTTGCCTCTAACTTATCTATGGAAAGGTTGCTTAAAGTGCATATACACAGAGGCAAAGAGAGAGAATTAATGTACACAGG

mRNA sequence

GATGGATGAAGAACACATCCCCAATGGCTTCGCAAAATCACAAATCTGATTGCCCTAACTTCAGCAAAAATCAGTTGAACAGAGCGATTTCGATGTAGAAGGAGACAACACCAGGTGTTTCCAATGCCAATAATTTAGGGCAAAATAGCGATTATTAAAGGAGCAATGAAAATTGAAGAAGAGGAGAAAGAAAGAAGCATTTGCCCTAAGTTTCAGATTCATTCCAAATCAAAGTTAAGTGCAGAAAATGGCGGACAGTGGCGTAAGCTCAGCCGAATGCGAGAGGATTTTCAAGCGATTCGATGCTAATGGCGACGGGAAGATCTCGGCGACGGAGCTGGGCGATGCTTTGAACGGATTTGGAGTGAACGCTGAGGACGCCAAGAGGATGATGGACGCCATTGATAAAGATGGCGATGGCTGCATTTCCTTTGAAGAGTTCGCCGATTTTGCTAAGGATAATCGCGCTTTGATGAAGGATTTTGCCAAGGCGTTTTAGTTCTCCATTGCCTTGCCACTTGTTCTCGATGGTGCCCTCACTGGTAGTCTCCGTTTCAGAAAAAGTGGCATCGGCTTGAGAGTGGTTGCCTCTAACTTATCTATGGAAAGGTTGCTTGGCTTGAGAGTGGTTGCCTCTAACTTATCTATGGAAAGGTTGCTTAAAGTGCATATACACAGAGGCAAAGAGAGAGAATTAATGTACACAGG

Coding sequence (CDS)

ATGGCGGACAGTGGCGTAAGCTCAGCCGAATGCGAGAGGATTTTCAAGCGATTCGATGCTAATGGCGACGGGAAGATCTCGGCGACGGAGCTGGGCGATGCTTTGAACGGATTTGGAGTGAACGCTGAGGACGCCAAGAGGATGATGGACGCCATTGATAAAGATGGCGATGGCTGCATTTCCTTTGAAGAGTTCGCCGATTTTGCTAAGGATAATCGCGCTTTGATGAAGGATTTTGCCAAGGCGTTTTAG

Protein sequence

MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCISFEEFADFAKDNRALMKDFAKAF

Homology

[Close]

BLAST of Lsi05G003120 vs. ExPASy Swiss-Prot
Match: Q9SRP7 (Probable calcium-binding protein CML28 OS=Arabidopsis thaliana OX=3702 GN=CML28 PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 7.1e-21
Identity = 56/84 (66.67%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFG-VNAEDAKRMMDAIDKDGDGC 60
          MAD+    AE +RIFK+FDANGDGKISA ELGDAL   G V  ED KRMM  ID DGDG 
Sbjct: 1  MADA-TEKAEHDRIFKKFDANGDGKISAAELGDALKNLGSVTHEDIKRMMAEIDTDGDGY 60

Query: 61 ISFEEFADFAKDNRALMKDFAKAF 84
          IS++EF DFA  NR LMKD AK F
Sbjct: 61 ISYQEFIDFASANRGLMKDVAKIF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy Swiss-Prot
Match: P69199 (Polcalcin Bra r 2 OS=Brassica campestris OX=3711 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 7.1e-21
Identity = 55/84 (65.48%), Postives = 63/84 (75.00%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFG-VNAEDAKRMMDAIDKDGDGC 60
          MAD+    AE +RIFK+FDANGDGKISA+ELGDAL   G V  +D KRMM  ID DGDG 
Sbjct: 1  MADA-TEKAEHDRIFKKFDANGDGKISASELGDALKNLGSVTHDDIKRMMAEIDTDGDGY 60

Query: 61 ISFEEFADFAKDNRALMKDFAKAF 84
          IS++EF+DFA  NR LMKD AK F
Sbjct: 61 ISYQEFSDFASANRGLMKDVAKIF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy Swiss-Prot
Match: P69198 (Polcalcin Bra n 2 OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 7.1e-21
Identity = 55/84 (65.48%), Postives = 63/84 (75.00%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFG-VNAEDAKRMMDAIDKDGDGC 60
          MAD+    AE +RIFK+FDANGDGKISA+ELGDAL   G V  +D KRMM  ID DGDG 
Sbjct: 1  MADA-TEKAEHDRIFKKFDANGDGKISASELGDALKNLGSVTHDDIKRMMAEIDTDGDGY 60

Query: 61 ISFEEFADFAKDNRALMKDFAKAF 84
          IS++EF+DFA  NR LMKD AK F
Sbjct: 61 ISYQEFSDFASANRGLMKDVAKIF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy Swiss-Prot
Match: Q9LF54 (Probable calcium-binding protein CML29 OS=Arabidopsis thaliana OX=3702 GN=CML29 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.8e-19
Identity = 54/84 (64.29%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFG-VNAEDAKRMMDAIDKDGDGC 60
          MAD+    AE +RIFK+FDANGDGKISA EL +AL   G V A+D KRMM  ID DGDG 
Sbjct: 1  MADA-TEKAEHDRIFKKFDANGDGKISAAELEEALKTLGSVTADDVKRMMAEIDTDGDGN 60

Query: 61 ISFEEFADFAKDNRALMKDFAKAF 84
          IS++EF DFA  NR LMKD AK F
Sbjct: 61 ISYQEFTDFAGANRGLMKDVAKIF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy Swiss-Prot
Match: O81092 (Polcalcin Ole e 3 OS=Olea europaea OX=4146 GN=OLE3 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.3e-19
Identity = 53/84 (63.10%), Postives = 60/84 (71.43%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFG-VNAEDAKRMMDAIDKDGDGC 60
          MAD     AE ERIFKRFDANGDGKIS++ELG+ L   G V  E+ +RMM  ID DGDG 
Sbjct: 1  MADDPQEVAEHERIFKRFDANGDGKISSSELGETLKTLGSVTPEEIQRMMAEIDTDGDGF 60

Query: 61 ISFEEFADFAKDNRALMKDFAKAF 84
          ISFEEF  FA+ NR L+KD AK F
Sbjct: 61 ISFEEFTVFARANRGLVKDVAKIF 84

[Close]

BLAST of Lsi05G003120 vs. ExPASy TrEMBL
Match: A0A1S4E1G1 (polcalcin Bra n 2-like OS=Cucumis melo OX=3656 GN=LOC103496835 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-34
Identity = 76/83 (91.57%), Postives = 82/83 (98.80%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MADSGV+SA+CERIFKRFDANGDGKISATELGDALNGFGV++EDAKRMMDAIDKDGDG I
Sbjct: 1  MADSGVNSADCERIFKRFDANGDGKISATELGDALNGFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SF+EF+DFAKDNRALMKDFAKAF
Sbjct: 61 SFQEFSDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy TrEMBL
Match: A0A6J1KB11 (probable calcium-binding protein CML28 OS=Cucurbita maxima OX=3661 GN=LOC111493300 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-32
Identity = 73/83 (87.95%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MAD+GV SAECERIFKRFDANGDG+ISATELGDALNG GV++EDAKRMMDAIDKDGDG I
Sbjct: 1  MADNGVDSAECERIFKRFDANGDGQISATELGDALNGIGVSSEDAKRMMDAIDKDGDGFI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SF+EF +FAKDNRALMKDFAKAF
Sbjct: 61 SFQEFFEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy TrEMBL
Match: A0A6J1G9I0 (probable calcium-binding protein CML28 OS=Cucurbita moschata OX=3662 GN=LOC111452170 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-32
Identity = 73/83 (87.95%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MAD+GV SAECERIFKRFDANGDG+ISATELGDALNG GV++EDAKRMMDAIDKDGDG I
Sbjct: 1  MADNGVDSAECERIFKRFDANGDGQISATELGDALNGIGVSSEDAKRMMDAIDKDGDGFI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SF+EF +FAKDNRALMKDFAKAF
Sbjct: 61 SFQEFFEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy TrEMBL
Match: A0A0A0L775 (Putative calcium-binding protein CML28 OS=Cucumis sativus OX=3659 GN=Csa_3G345360 PE=4 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.2e-32
Identity = 74/83 (89.16%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MADSGV+SA+ ERIFKRFDANGDGKISATELGDALN FGV++EDAKRMMDAIDKDGDG I
Sbjct: 1  MADSGVNSADLERIFKRFDANGDGKISATELGDALNEFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SF+EF DFAKDNRALMKDFAKAF
Sbjct: 61 SFQEFFDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. ExPASy TrEMBL
Match: A0A6J1J5E7 (polcalcin Syr v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481518 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-31
Identity = 71/83 (85.54%), Postives = 77/83 (92.77%), Query Frame = 0


Query: 1   MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
           MA+S +S+AECERIFKRFDANGDG ISA ELGDALNG GVNAE+AKRMMDAIDKDGDG I
Sbjct: 30  MAESDISAAECERIFKRFDANGDGMISAAELGDALNGLGVNAEEAKRMMDAIDKDGDGFI 89

Query: 61  SFEEFADFAKDNRALMKDFAKAF 84
           S+EEFADF K+NRALMKDFAKAF
Sbjct: 90  SWEEFADFTKENRALMKDFAKAF 112

[Close]

BLAST of Lsi05G003120 vs. NCBI nr
Match: XP_038874793.1 (probable calcium-binding protein CML28 [Benincasa hispida])

HSP 1 Score: 159.8 bits (403), Expect = 9.8e-36
Identity = 78/83 (93.98%), Postives = 83/83 (100.00%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MA+SGVS+A+CERIFKRFDANGDGKISATELGDALNGFGV+AEDAKRMMDAIDKDGDGCI
Sbjct: 1  MAESGVSAADCERIFKRFDANGDGKISATELGDALNGFGVSAEDAKRMMDAIDKDGDGCI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SFEEFA+FAKDNRALMKDFAKAF
Sbjct: 61 SFEEFAEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. NCBI nr
Match: XP_016902062.1 (PREDICTED: polcalcin Bra n 2-like [Cucumis melo])

HSP 1 Score: 155.2 bits (391), Expect = 2.4e-34
Identity = 76/83 (91.57%), Postives = 82/83 (98.80%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MADSGV+SA+CERIFKRFDANGDGKISATELGDALNGFGV++EDAKRMMDAIDKDGDG I
Sbjct: 1  MADSGVNSADCERIFKRFDANGDGKISATELGDALNGFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SF+EF+DFAKDNRALMKDFAKAF
Sbjct: 61 SFQEFSDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. NCBI nr
Match: XP_022948513.1 (probable calcium-binding protein CML28 [Cucurbita moschata] >XP_022998721.1 probable calcium-binding protein CML28 [Cucurbita maxima] >XP_023523664.1 probable calcium-binding protein CML28 [Cucurbita pepo subsp. pepo] >KAG6607008.1 Polcalcin Nic t 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036707.1 Polcalcin Nic t 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 148.3 bits (373), Expect = 3.0e-32
Identity = 73/83 (87.95%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MAD+GV SAECERIFKRFDANGDG+ISATELGDALNG GV++EDAKRMMDAIDKDGDG I
Sbjct: 1  MADNGVDSAECERIFKRFDANGDGQISATELGDALNGIGVSSEDAKRMMDAIDKDGDGFI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SF+EF +FAKDNRALMKDFAKAF
Sbjct: 61 SFQEFFEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. NCBI nr
Match: XP_004149790.1 (probable calcium-binding protein CML28 [Cucumis sativus] >KGN57835.1 hypothetical protein Csa_010503 [Cucumis sativus])

HSP 1 Score: 147.1 bits (370), Expect = 6.6e-32
Identity = 74/83 (89.16%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
          MADSGV+SA+ ERIFKRFDANGDGKISATELGDALN FGV++EDAKRMMDAIDKDGDG I
Sbjct: 1  MADSGVNSADLERIFKRFDANGDGKISATELGDALNEFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 61 SFEEFADFAKDNRALMKDFAKAF 84
          SF+EF DFAKDNRALMKDFAKAF
Sbjct: 61 SFQEFFDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Lsi05G003120 vs. NCBI nr
Match: XP_022982739.1 (polcalcin Syr v 3-like [Cucurbita maxima])

HSP 1 Score: 144.1 bits (362), Expect = 5.6e-31
Identity = 71/83 (85.54%), Postives = 77/83 (92.77%), Query Frame = 0


Query: 1   MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFGVNAEDAKRMMDAIDKDGDGCI 60
           MA+S +S+AECERIFKRFDANGDG ISA ELGDALNG GVNAE+AKRMMDAIDKDGDG I
Sbjct: 30  MAESDISAAECERIFKRFDANGDGMISAAELGDALNGLGVNAEEAKRMMDAIDKDGDGFI 89

Query: 61  SFEEFADFAKDNRALMKDFAKAF 84
           S+EEFADF K+NRALMKDFAKAF
Sbjct: 90  SWEEFADFTKENRALMKDFAKAF 112

[Close]

BLAST of Lsi05G003120 vs. TAIR 10
Match: AT3G03430.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 100.9 bits (250), Expect = 5.1e-22
Identity = 56/84 (66.67%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFG-VNAEDAKRMMDAIDKDGDGC 60
          MAD+    AE +RIFK+FDANGDGKISA ELGDAL   G V  ED KRMM  ID DGDG 
Sbjct: 1  MADA-TEKAEHDRIFKKFDANGDGKISAAELGDALKNLGSVTHEDIKRMMAEIDTDGDGY 60

Query: 61 ISFEEFADFAKDNRALMKDFAKAF 84
          IS++EF DFA  NR LMKD AK F
Sbjct: 61 ISYQEFIDFASANRGLMKDVAKIF 83

[Close]

BLAST of Lsi05G003120 vs. TAIR 10
Match: AT5G17480.1 (pollen calcium-binding protein 1 )

HSP 1 Score: 96.3 bits (238), Expect = 1.2e-20
Identity = 54/84 (64.29%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 1  MADSGVSSAECERIFKRFDANGDGKISATELGDALNGFG-VNAEDAKRMMDAIDKDGDGC 60
          MAD+    AE +RIFK+FDANGDGKISA EL +AL   G V A+D KRMM  ID DGDG 
Sbjct: 1  MADA-TEKAEHDRIFKKFDANGDGKISAAELEEALKTLGSVTADDVKRMMAEIDTDGDGN 60

Query: 61 ISFEEFADFAKDNRALMKDFAKAF 84
          IS++EF DFA  NR LMKD AK F
Sbjct: 61 ISYQEFTDFAGANRGLMKDVAKIF 83

[Close]

BLAST of Lsi05G003120 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 58.9 bits (141), Expect = 2.2e-09
Identity = 32/79 (40.51%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 10  ECERIFKRFDANGDGKISATELGDALNGFG--VNAEDAKRMMDAIDKDGDGCISFEEFAD 69
           E E +FK+FD NGDGKIS+ ELG  +   G  V  E+ ++ +  ID+ GDG I+FEEF +
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 70  F---AKDNRALMKDFAKAF 84
                 D   ++++   AF
Sbjct: 97  LNTKGMDQNDVLENLKDAF 115

[Close]

BLAST of Lsi05G003120 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 56.2 bits (134), Expect = 1.4e-08
Identity = 25/62 (40.32%), Postives = 40/62 (64.52%), Query Frame = 0


Query: 6   VSSAECERIFKRFDANGDGKISATELGDALNGFGVNA--EDAKRMMDAIDKDGDGCISFE 65
           +  +E +R+F+ FD NGDG+I+  EL D+L   G+    +D  +M+  ID +GDGC+  +
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

[Close]

BLAST of Lsi05G003120 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 55.1 bits (131), Expect = 3.2e-08
Identity = 25/63 (39.68%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 10 ECERIFKRFDANGDGKISATELGDALNGFGVNAEDAK--RMMDAIDKDGDGCISFEEFAD 69
          E +R+F+ FD NGDG I+  EL + L   G+   D +  +M++ ID +GDGC+  +EF +
Sbjct: 5  ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 70 FAK 71
            K
Sbjct: 65 LYK 67

The following BLAST results are available for this feature:

BLAST of Lsi05G003120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRP7	7.1e-21	66.67	Probable calcium-binding protein CML28 OS=Arabidopsis thaliana OX=3702 GN=CML28 ...	[more]
P69199	7.1e-21	65.48	Polcalcin Bra r 2 OS=Brassica campestris OX=3711 PE=1 SV=1	[more]
P69198	7.1e-21	65.48	Polcalcin Bra n 2 OS=Brassica napus OX=3708 PE=1 SV=1	[more]
Q9LF54	1.8e-19	64.29	Probable calcium-binding protein CML29 OS=Arabidopsis thaliana OX=3702 GN=CML29 ...	[more]
O81092	2.3e-19	63.10	Polcalcin Ole e 3 OS=Olea europaea OX=4146 GN=OLE3 PE=1 SV=1	[more]

BLAST of Lsi05G003120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E1G1	1.2e-34	91.57	polcalcin Bra n 2-like OS=Cucumis melo OX=3656 GN=LOC103496835 PE=4 SV=1	[more]
A0A6J1KB11	1.4e-32	87.95	probable calcium-binding protein CML28 OS=Cucurbita maxima OX=3661 GN=LOC1114933...	[more]
A0A6J1G9I0	1.4e-32	87.95	probable calcium-binding protein CML28 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A0A0L775	3.2e-32	89.16	Putative calcium-binding protein CML28 OS=Cucumis sativus OX=3659 GN=Csa_3G34536...	[more]
A0A6J1J5E7	2.7e-31	85.54	polcalcin Syr v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481518 PE=4 SV=1	[more]

BLAST of Lsi05G003120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874793.1	9.8e-36	93.98	probable calcium-binding protein CML28 [Benincasa hispida]	[more]
XP_016902062.1	2.4e-34	91.57	PREDICTED: polcalcin Bra n 2-like [Cucumis melo]	[more]
XP_022948513.1	3.0e-32	87.95	probable calcium-binding protein CML28 [Cucurbita moschata] >XP_022998721.1 prob...	[more]
XP_004149790.1	6.6e-32	89.16	probable calcium-binding protein CML28 [Cucumis sativus] >KGN57835.1 hypothetica...	[more]
XP_022982739.1	5.6e-31	85.54	polcalcin Syr v 3-like [Cucurbita maxima]	[more]

BLAST of Lsi05G003120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03430.1	5.1e-22	66.67	Calcium-binding EF-hand family protein	[more]
AT5G17480.1	1.2e-20	64.29	pollen calcium-binding protein 1	[more]
AT1G24620.1	2.2e-09	40.51	EF hand calcium-binding protein family	[more]
AT2G43290.1	1.4e-08	40.32	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	3.2e-08	39.68	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 44..72 e-value: 4.4E-4 score: 29.6 coord: 10..38 e-value: 2.8E-4 score: 30.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 11..69 e-value: 1.7E-13 score: 50.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 6..41 score: 15.433954
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 43..75 score: 13.062845
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 10..70 e-value: 1.32269E-15 score: 62.5653
None	No IPR available	GENE3D	1.10.238.10		coord: 3..80 e-value: 1.6E-21 score: 78.8
None	No IPR available	PANTHER	PTHR23064	TROPONIN	coord: 1..83
None	No IPR available	PANTHER	PTHR23064:SF60	POLCALCIN NIC T 1	coord: 1..83
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 53..65
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 19..31
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 10..69

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G003120.1	Lsi05G003120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding