Lsi05G002220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G002220
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDDE Tnp4 domain-containing protein
Locationchr05: 2991641 .. 2992600 (+)
RNA-Seq ExpressionLsi05G002220
SyntenyLsi05G002220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCTCCTCAACGTTTGAATGGCTCTCTGGTTTACTTGAGCCCCTTCTGGAGTGTCGCGACCCGGTGGGTTCGCCTCTCGATCTCTCCGTTGAGATTCGGCTCGGTGTTGGTCTGTATCGCCTCGCCACCGGCTGCGATTTCTCCACAATCTCGGACCAATTTGGCGTCTCGGAGTCGGTAGCGAGGTTCTGTGCTAAACAATTGTGTCGAGTTCTTTGTACTAATTTTCGCTTCTGGGTCGAATTCCCTTGCCCCAATGAGCTCGAATTAACGTCCTCGGCTTTTGAAGATCTTGCTGGACTTCCGAATTGCTGTGGCGTGGTTTCTTGTACAAGGTTCAAGATCATTAGAAATAGCCATTTTTATGAAGATAGCATCGCTACTCAACTTGTTGTTGATTCCTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGCGATAAGGACGATTCCACAGTGCTTATGTCCTCGACGCTGTTTAAAGACATTGAACAAGGAAGGCTTCTGGATGCTCCTCCGGTTTACCTTCATGGGGTGGCTGTGAATCAGTACTTGTTTGGACATGGTGAATACCCTTTGCTTCCATGGTTAATAGTGCCTTTTGCAGGAGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATGAAGCTCACCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGCCAACCAATTCATGAGGAGTTCAAAACTGCTGTTGCTTACATTGGTGCTTGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGATTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTAGCTTCATTTGATCATAAATCTCAGTATGTTAAAGCTGGGTTGAATGAGGATTCAACTAATGAGAAGGCTTCTGTTATACAGAGGGCATTGGCTCTGAGAGCTAGAGAGCTTCACAGTTAA

mRNA sequence

ATGACCTCCTCAACGTTTGAATGGCTCTCTGGTTTACTTGAGCCCCTTCTGGAGTGTCGCGACCCGGTGGGTTCGCCTCTCGATCTCTCCGTTGAGATTCGGCTCGGTGTTGGTCTGTATCGCCTCGCCACCGGCTGCGATTTCTCCACAATCTCGGACCAATTTGGCGTCTCGGAGTCGGTAGCGAGGTTCTGTGCTAAACAATTGTGTCGAGTTCTTTGTACTAATTTTCGCTTCTGGGTCGAATTCCCTTGCCCCAATGAGCTCGAATTAACGTCCTCGGCTTTTGAAGATCTTGCTGGACTTCCGAATTGCTGTGGCGTGGTTTCTTGTACAAGGTTCAAGATCATTAGAAATAGCCATTTTTATGAAGATAGCATCGCTACTCAACTTGTTGTTGATTCCTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGCGATAAGGACGATTCCACAGTGCTTATGTCCTCGACGCTGTTTAAAGACATTGAACAAGGAAGGCTTCTGGATGCTCCTCCGGTTTACCTTCATGGGGTGGCTGTGAATCAGTACTTGTTTGGACATGGTGAATACCCTTTGCTTCCATGGTTAATAGTGCCTTTTGCAGGAGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATGAAGCTCACCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGCCAACCAATTCATGAGGAGTTCAAAACTGCTGTTGCTTACATTGGTGCTTGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGATTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTAGCTTCATTTGATCATAAATCTCAGTATGTTAAAGCTGGGTTGAATGAGGATTCAACTAATGAGAAGGCTTCTGTTATACAGAGGGCATTGGCTCTGAGAGCTAGAGAGCTTCACAGTTAA

Coding sequence (CDS)

ATGACCTCCTCAACGTTTGAATGGCTCTCTGGTTTACTTGAGCCCCTTCTGGAGTGTCGCGACCCGGTGGGTTCGCCTCTCGATCTCTCCGTTGAGATTCGGCTCGGTGTTGGTCTGTATCGCCTCGCCACCGGCTGCGATTTCTCCACAATCTCGGACCAATTTGGCGTCTCGGAGTCGGTAGCGAGGTTCTGTGCTAAACAATTGTGTCGAGTTCTTTGTACTAATTTTCGCTTCTGGGTCGAATTCCCTTGCCCCAATGAGCTCGAATTAACGTCCTCGGCTTTTGAAGATCTTGCTGGACTTCCGAATTGCTGTGGCGTGGTTTCTTGTACAAGGTTCAAGATCATTAGAAATAGCCATTTTTATGAAGATAGCATCGCTACTCAACTTGTTGTTGATTCCTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGCGATAAGGACGATTCCACAGTGCTTATGTCCTCGACGCTGTTTAAAGACATTGAACAAGGAAGGCTTCTGGATGCTCCTCCGGTTTACCTTCATGGGGTGGCTGTGAATCAGTACTTGTTTGGACATGGTGAATACCCTTTGCTTCCATGGTTAATAGTGCCTTTTGCAGGAGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATGAAGCTCACCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGCCAACCAATTCATGAGGAGTTCAAAACTGCTGTTGCTTACATTGGTGCTTGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGATTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTAGCTTCATTTGATCATAAATCTCAGTATGTTAAAGCTGGGTTGAATGAGGATTCAACTAATGAGAAGGCTTCTGTTATACAGAGGGCATTGGCTCTGAGAGCTAGAGAGCTTCACAGTTAA

Protein sequence

MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTNEKASVIQRALALRARELHS
Homology
BLAST of Lsi05G002220 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.7e-26
Identity = 82/294 (27.89%), Postives = 140/294 (47.62%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECR-----DPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQF 60
           ++  TF+++  L++     +     D  G+P  LS+  R+ V L RL +G   S I + F
Sbjct: 78  ISRKTFDYICSLVKADFTAKPANFSDSNGNP--LSLNDRVAVALRRLGSGESLSVIGETF 137

Query: 61  GVSESVA-----RFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVS 120
           G+++S       RF      R +  +   W     P++L+   S FE ++GLPNCCG + 
Sbjct: 138 GMNQSTVSQITWRFVESMEERAI--HHLSW-----PSKLDEIKSKFEKISGLPNCCGAID 197

Query: 121 CTRFKIIRNSHFYED------------SIATQLVVDSSSRILSIVAGFRGDKDDSTVLMS 180
            T   I+ N    E             S+  Q VVD   R L ++AG+ G  +D  VL +
Sbjct: 198 ITH--IVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLNDDVVLKN 257

Query: 181 STLFKDIEQGRLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNE 240
           S  +K +E+G+ L+   + L     + +Y+ G   +PLLPWL+ P+ G  +   +  FN+
Sbjct: 258 SGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPTSLPQTEFNK 317

Query: 241 AHRLMCIPALKAIVSLRN-WGVLSQPIHEEFKTAV-AYIGACSILHNALLMRED 270
            H      A  A+  L++ W +++  +    +  +   I  C +LHN ++  ED
Sbjct: 318 RHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIIDMED 360

BLAST of Lsi05G002220 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 9.7e-26
Identity = 83/285 (29.12%), Postives = 132/285 (46.32%), Query Frame = 0

Query: 2   TSSTFEWLSGLLEPLLECRDPVG----SPLDLSVEIRLGVGLYRLATGCDFSTISDQFGV 61
           + +TF ++  L+   L  R P G        LSVE ++ + L RLA+G    ++   FGV
Sbjct: 72  SKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQVSVGAAFGV 131

Query: 62  SESVARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTR---- 121
            +S       +    L    +  + +P  + +E   S FE++ GLPNCCG +  T     
Sbjct: 132 GQSTVSQVTWRFIEALEERAKHHLRWPDSDRIEEIKSKFEEMYGLPNCCGAIDTTHIIMT 191

Query: 122 FKIIRNSHFYED-----SIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQG 181
              ++ S  + D     S+  Q V D   R L++V G+ G    S +L  S  FK  E  
Sbjct: 192 LPAVQASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENA 251

Query: 182 RLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPAL 241
           ++LD  P  L  G  + +Y+ G   YPLLPWLI P        +  +FNE H  +   A 
Sbjct: 252 QILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAA 311

Query: 242 KAIVSLR-NWGVLSQPI-HEEFKTAVAYIGACSILHNALLMREDF 271
            A   L+ +W +LS+ +   + +   + I  C +LHN ++   D+
Sbjct: 312 TAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 356

BLAST of Lsi05G002220 vs. ExPASy TrEMBL
Match: A0A0A0LBX6 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G202740 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 8.7e-179
Identity = 309/319 (96.87%), Postives = 317/319 (99.37%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES
Sbjct: 106 MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 165

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFC+KQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS
Sbjct: 166 VARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 225

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
           HFYEDS+ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHG
Sbjct: 226 HFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHG 285

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           VAVN+YLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS
Sbjct: 286 VAVNKYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 345

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESL+S DHKSQYV+AGLN DSTN
Sbjct: 346 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHKSQYVEAGLNVDSTN 405

Query: 301 EKASVIQRALALRARELHS 320
           EKASVIQRALALRARELHS
Sbjct: 406 EKASVIQRALALRARELHS 424

BLAST of Lsi05G002220 vs. ExPASy TrEMBL
Match: A0A5D3CRB2 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001410 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 8.2e-177
Identity = 306/319 (95.92%), Postives = 315/319 (98.75%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES
Sbjct: 106 MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 165

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFC+KQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS
Sbjct: 166 VARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 225

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
           HFYEDS+ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHG
Sbjct: 226 HFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHG 285

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           VAVN+YLFG GEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS
Sbjct: 286 VAVNKYLFGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 345

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESL+S DH+SQYV+AGLN DSTN
Sbjct: 346 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTN 405

Query: 301 EKASVIQRALALRARELHS 320
           EKASVIQRALA RARELHS
Sbjct: 406 EKASVIQRALAQRARELHS 424

BLAST of Lsi05G002220 vs. ExPASy TrEMBL
Match: A0A1S3C2M8 (uncharacterized protein LOC103496169 OS=Cucumis melo OX=3656 GN=LOC103496169 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 8.2e-177
Identity = 306/319 (95.92%), Postives = 315/319 (98.75%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES
Sbjct: 63  MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 122

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFC+KQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS
Sbjct: 123 VARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 182

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
           HFYEDS+ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHG
Sbjct: 183 HFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHG 242

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           VAVN+YLFG GEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS
Sbjct: 243 VAVNKYLFGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 302

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESL+S DH+SQYV+AGLN DSTN
Sbjct: 303 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTN 362

Query: 301 EKASVIQRALALRARELHS 320
           EKASVIQRALA RARELHS
Sbjct: 363 EKASVIQRALAQRARELHS 381

BLAST of Lsi05G002220 vs. ExPASy TrEMBL
Match: A0A6J1D7F1 (protein ALP1-like OS=Momordica charantia OX=3673 GN=LOC111017620 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.7e-150
Identity = 268/319 (84.01%), Postives = 280/319 (87.77%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPL+LS EIRLGVGL RLATGCDFSTIS+QFGVSES
Sbjct: 115 MTSSTFEWLSGLLEPLLECRDPVGSPLNLSAEIRLGVGLSRLATGCDFSTISEQFGVSES 174

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFCAKQLCRVLCTNFRFWVEFPCPNELE TSSAFE LAGLPNCCGVV+CT        
Sbjct: 175 VARFCAKQLCRVLCTNFRFWVEFPCPNELESTSSAFEALAGLPNCCGVVACT-------- 234

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
                               SIVAGFRGDKDDSTVLMSSTLFKDIE+GRLLD+PPVYLHG
Sbjct: 235 --------------------SIVAGFRGDKDDSTVLMSSTLFKDIEEGRLLDSPPVYLHG 294

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           +AVNQY FGHGEYPLLPWL+VPF+GAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS
Sbjct: 295 MAVNQYFFGHGEYPLLPWLMVPFSGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLS 354

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QP+ EEFKTAVAYIGACSILHNALLMREDFSAMADEWE LAS DH SQY+  GLNEDST+
Sbjct: 355 QPMREEFKTAVAYIGACSILHNALLMREDFSAMADEWEGLASLDHGSQYIGEGLNEDSTD 405

Query: 301 EKASVIQRALALRARELHS 320
           EKASVIQRALALRARELHS
Sbjct: 415 EKASVIQRALALRARELHS 405

BLAST of Lsi05G002220 vs. ExPASy TrEMBL
Match: A0A6J1FNZ2 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446927 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.2e-150
Identity = 264/319 (82.76%), Postives = 282/319 (88.40%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFSTISDQFGVSES
Sbjct: 116 MTSSTFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSES 175

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFCAKQLCRVLCTNFRFWVEFPCP+ELELTSS+FED+AGLPNCCGV+SCT        
Sbjct: 176 VARFCAKQLCRVLCTNFRFWVEFPCPSELELTSSSFEDIAGLPNCCGVISCT-------- 235

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
                               SIVAGFRGDKDDSTVLMS+TLFKDIE+ RLL +PPVYLHG
Sbjct: 236 --------------------SIVAGFRGDKDDSTVLMSTTLFKDIEEERLLGSPPVYLHG 295

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           +AVNQYLFGHGEYPLLPWL+VPFAGAVSGSTEESFNEAHRLMCIPALKAI+SLRNWGVLS
Sbjct: 296 MAVNQYLFGHGEYPLLPWLMVPFAGAVSGSTEESFNEAHRLMCIPALKAIISLRNWGVLS 355

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QP+HEEFKTAVAYIGACSILHNALLMREDF+AMADEWESLAS DH SQYV  GLNEDS +
Sbjct: 356 QPMHEEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPD 406

Query: 301 EKASVIQRALALRARELHS 320
           EKA+++Q+ALALRARELH+
Sbjct: 416 EKATMLQKALALRARELHT 406

BLAST of Lsi05G002220 vs. NCBI nr
Match: KGN57516.1 (hypothetical protein Csa_011580 [Cucumis sativus])

HSP 1 Score: 636.0 bits (1639), Expect = 1.8e-178
Identity = 309/319 (96.87%), Postives = 317/319 (99.37%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES
Sbjct: 106 MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 165

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFC+KQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS
Sbjct: 166 VARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 225

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
           HFYEDS+ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHG
Sbjct: 226 HFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHG 285

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           VAVN+YLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS
Sbjct: 286 VAVNKYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 345

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESL+S DHKSQYV+AGLN DSTN
Sbjct: 346 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHKSQYVEAGLNVDSTN 405

Query: 301 EKASVIQRALALRARELHS 320
           EKASVIQRALALRARELHS
Sbjct: 406 EKASVIQRALALRARELHS 424

BLAST of Lsi05G002220 vs. NCBI nr
Match: KAA0037135.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK13940.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 629.4 bits (1622), Expect = 1.7e-176
Identity = 306/319 (95.92%), Postives = 315/319 (98.75%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES
Sbjct: 106 MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 165

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFC+KQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS
Sbjct: 166 VARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 225

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
           HFYEDS+ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHG
Sbjct: 226 HFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHG 285

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           VAVN+YLFG GEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS
Sbjct: 286 VAVNKYLFGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 345

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESL+S DH+SQYV+AGLN DSTN
Sbjct: 346 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTN 405

Query: 301 EKASVIQRALALRARELHS 320
           EKASVIQRALA RARELHS
Sbjct: 406 EKASVIQRALAQRARELHS 424

BLAST of Lsi05G002220 vs. NCBI nr
Match: XP_008456140.1 (PREDICTED: uncharacterized protein LOC103496169 [Cucumis melo])

HSP 1 Score: 629.4 bits (1622), Expect = 1.7e-176
Identity = 306/319 (95.92%), Postives = 315/319 (98.75%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES
Sbjct: 63  MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 122

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFC+KQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS
Sbjct: 123 VARFCSKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 182

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
           HFYEDS+ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHG
Sbjct: 183 HFYEDSVATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHG 242

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           VAVN+YLFG GEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS
Sbjct: 243 VAVNKYLFGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 302

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESL+S DH+SQYV+AGLN DSTN
Sbjct: 303 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTN 362

Query: 301 EKASVIQRALALRARELHS 320
           EKASVIQRALA RARELHS
Sbjct: 363 EKASVIQRALAQRARELHS 381

BLAST of Lsi05G002220 vs. NCBI nr
Match: XP_038880641.1 (protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1-like [Benincasa hispida])

HSP 1 Score: 561.2 bits (1445), Expect = 5.6e-156
Identity = 277/319 (86.83%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFGVSES
Sbjct: 106 MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSES 165

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CT        
Sbjct: 166 VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCT-------- 225

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
                               SIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG
Sbjct: 226 --------------------SIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 285

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           VAVNQYLFGHGEYPLLPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS
Sbjct: 286 VAVNQYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLS 345

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           +P+HEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDH+SQYV+  LNEDSTN
Sbjct: 346 KPMHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTN 396

Query: 301 EKASVIQRALALRARELHS 320
           EKAS+IQRALA+RARELHS
Sbjct: 406 EKASIIQRALAVRARELHS 396

BLAST of Lsi05G002220 vs. NCBI nr
Match: XP_023536005.1 (protein ALP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.7 bits (1410), Expect = 6.4e-152
Identity = 268/319 (84.01%), Postives = 283/319 (88.71%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSES 60
           MTSSTFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFSTISDQFGVSES
Sbjct: 116 MTSSTFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSES 175

Query: 61  VARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS 120
           VARFCAKQLCRVLCTNFRFWVEFPCP+ELELTSSAFED+AGLPNCCGV+SCT        
Sbjct: 176 VARFCAKQLCRVLCTNFRFWVEFPCPSELELTSSAFEDIAGLPNCCGVISCT-------- 235

Query: 121 HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHG 180
                               SIVAGFRGDKDDSTVLMS+TLFKDIE+GRLL +PPVYLHG
Sbjct: 236 --------------------SIVAGFRGDKDDSTVLMSTTLFKDIEEGRLLGSPPVYLHG 295

Query: 181 VAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLS 240
           +AVNQYLFGHGEYPLLPWL+VPFAGAVSGSTEESFNEAHRLMCIPALKAI+SLRNWGVLS
Sbjct: 296 MAVNQYLFGHGEYPLLPWLMVPFAGAVSGSTEESFNEAHRLMCIPALKAIISLRNWGVLS 355

Query: 241 QPIHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHKSQYVKAGLNEDSTN 300
           QP+HEEFKTAVAYIGACSILHNALLMREDF+AMADEWESLAS DH SQYV  GLNEDS +
Sbjct: 356 QPMHEEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPD 406

Query: 301 EKASVIQRALALRARELHS 320
           EKAS+IQ+ALALRARELH+
Sbjct: 416 EKASMIQKALALRARELHT 406

BLAST of Lsi05G002220 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 119.8 bits (299), Expect = 4.0e-27
Identity = 82/294 (27.89%), Postives = 140/294 (47.62%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLECR-----DPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQF 60
           ++  TF+++  L++     +     D  G+P  LS+  R+ V L RL +G   S I + F
Sbjct: 78  ISRKTFDYICSLVKADFTAKPANFSDSNGNP--LSLNDRVAVALRRLGSGESLSVIGETF 137

Query: 61  GVSESVA-----RFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVS 120
           G+++S       RF      R +  +   W     P++L+   S FE ++GLPNCCG + 
Sbjct: 138 GMNQSTVSQITWRFVESMEERAI--HHLSW-----PSKLDEIKSKFEKISGLPNCCGAID 197

Query: 121 CTRFKIIRNSHFYED------------SIATQLVVDSSSRILSIVAGFRGDKDDSTVLMS 180
            T   I+ N    E             S+  Q VVD   R L ++AG+ G  +D  VL +
Sbjct: 198 ITH--IVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLNDDVVLKN 257

Query: 181 STLFKDIEQGRLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNE 240
           S  +K +E+G+ L+   + L     + +Y+ G   +PLLPWL+ P+ G  +   +  FN+
Sbjct: 258 SGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPTSLPQTEFNK 317

Query: 241 AHRLMCIPALKAIVSLRN-WGVLSQPIHEEFKTAV-AYIGACSILHNALLMRED 270
            H      A  A+  L++ W +++  +    +  +   I  C +LHN ++  ED
Sbjct: 318 RHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIIDMED 360

BLAST of Lsi05G002220 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 119.0 bits (297), Expect = 6.9e-27
Identity = 83/285 (29.12%), Postives = 132/285 (46.32%), Query Frame = 0

Query: 2   TSSTFEWLSGLLEPLLECRDPVG----SPLDLSVEIRLGVGLYRLATGCDFSTISDQFGV 61
           + +TF ++  L+   L  R P G        LSVE ++ + L RLA+G    ++   FGV
Sbjct: 72  SKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQVSVGAAFGV 131

Query: 62  SESVARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTR---- 121
            +S       +    L    +  + +P  + +E   S FE++ GLPNCCG +  T     
Sbjct: 132 GQSTVSQVTWRFIEALEERAKHHLRWPDSDRIEEIKSKFEEMYGLPNCCGAIDTTHIIMT 191

Query: 122 FKIIRNSHFYED-----SIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQG 181
              ++ S  + D     S+  Q V D   R L++V G+ G    S +L  S  FK  E  
Sbjct: 192 LPAVQASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENA 251

Query: 182 RLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPAL 241
           ++LD  P  L  G  + +Y+ G   YPLLPWLI P        +  +FNE H  +   A 
Sbjct: 252 QILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAA 311

Query: 242 KAIVSLR-NWGVLSQPI-HEEFKTAVAYIGACSILHNALLMREDF 271
            A   L+ +W +LS+ +   + +   + I  C +LHN ++   D+
Sbjct: 312 TAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 356

BLAST of Lsi05G002220 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 92.8 bits (229), Expect = 5.3e-19
Identity = 64/252 (25.40%), Postives = 111/252 (44.05%), Query Frame = 0

Query: 25  SPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCAKQLCRVLCTN-FRFWVEF 84
           S L L  +  + + L RLA GC   T++ ++ +   +       + R+L T  +  +++ 
Sbjct: 142 SNLSLPADYAVAMVLSRLAHGCSAKTLASRYSLDPYLISKITNMVTRLLATKLYPEFIKI 201

Query: 85  PC-PNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNS----------HFYEDSIATQLV 144
           P     L  T+  FE+L  LPN CG +  T  K+ R +           +  D++  Q+V
Sbjct: 202 PVGKRRLIETTQGFEELTSLPNICGAIDSTPVKLRRRTKLNPRNIYGCKYGYDAVLLQVV 261

Query: 145 VDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGE 204
            D       +     G +DDS+    S L+K +  G ++    + + G  V  Y+ G   
Sbjct: 262 ADHKKIFWDVCVKAPGGEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGHHVRPYIVGDWC 321

Query: 205 YPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSL--RNWGVLSQPIHEEFKTA 263
           YPLL +L+ PF+   SG+  E+  +   +     +   + L    W +L Q ++     A
Sbjct: 322 YPLLSFLMTPFSPNGSGTPPENLFDGMLMKGRSVVVEAIGLLKARWKIL-QSLNVGVNHA 381

BLAST of Lsi05G002220 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 86.3 bits (212), Expect = 5.0e-17
Identity = 75/295 (25.42%), Postives = 124/295 (42.03%), Query Frame = 0

Query: 1   MTSSTFEWLSGLLEPLLE-----CRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQF 60
           M+ STF  +   L+  +       RD + +P       R+GV ++RLATG     +S++F
Sbjct: 219 MSKSTFNLICEELDTTVTKKNTMLRDAIPAPK------RVGVCVWRLATGAPLRHVSERF 278

Query: 61  GVSESVARFCAKQLCR----VLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSC 120
           G+  S       ++CR    VL   +  W   P  +E+  T + FE +  +PN  G +  
Sbjct: 279 GLGISTCHKLVIEVCRAIYDVLMPKYLLW---PSDSEINSTKAKFESVHKIPNVVGSIYT 338

Query: 121 TRFKIIR---------NSHFYED------SIATQLVVDSSSRILSIVAGFRGDKDDSTVL 180
           T   II          N    E       SI  Q VV++      +  G  G   D  +L
Sbjct: 339 THIPIIAPKVHVAAYFNKRHTERNQKTSYSITVQGVVNADGIFTDVCIGNPGSLTDDQIL 398

Query: 181 MSSTLFKD-IEQGRLLDAPPVYLHGVAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESF 240
             S+L +    +G L D+            ++ G+  +PL  +L+VP+       T+ +F
Sbjct: 399 EKSSLSRQRAARGMLRDS------------WIVGNSGFPLTDYLLVPYTRQNLTWTQHAF 458

Query: 241 NEAHRLMCIPALKAIVSLR-NWGVLSQPIHEEFKTAVAYIGACSILHNALLMRED 270
           NE+   +   A  A   L+  W  L +    + +     +GAC +LHN   MR++
Sbjct: 459 NESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVLGACCVLHNICEMRKE 492

BLAST of Lsi05G002220 vs. TAIR 10
Match: AT1G72270.1 (CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR021714); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27010.1); Has 772 Blast hits to 657 proteins in 120 species: Archae - 0; Bacteria - 0; Metazoa - 344; Fungi - 94; Plants - 322; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). )

HSP 1 Score: 77.4 bits (189), Expect = 2.3e-14
Identity = 72/247 (29.15%), Postives = 106/247 (42.91%), Query Frame = 0

Query: 39  LYRLATGCDFSTISDQFGV-SESVARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFE 98
           ++RLA G  +  +  +FG  S S A      +C+++       ++ P P+        F 
Sbjct: 127 IFRLAHGASYECLVHRFGFDSTSQASRSFFTVCKLINEKLSQQLDDPKPD--------FS 186

Query: 99  DLAGLPNCCGVVSCTRFKIIRNSHFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLM 158
               LPNC GVV   RF++       + SI  Q +VDS+ R + I AG+        +  
Sbjct: 187 PNL-LPNCYGVVGFGRFEVKGKLLGAKGSILVQALVDSNGRFVDISAGWPSTMKPEAIFR 246

Query: 159 SSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPLLPWLIVPFAGAVSGSTEESFNE 218
            + LF  I +  L  AP    +GV V +Y+ G    PLLPWL+ P+      S EESF E
Sbjct: 247 QTKLF-SIAEEVLSGAPTKLGNGVLVPRYILGDSCLPLLPWLVTPYD---LTSDEESFRE 306

Query: 219 AHRLMCIPALK----AIVSLR-NWGVLS---QPIHEEFKTAVAYIGACSILHNALLMRED 277
               +    L     A   +R  W +L    +P   EF   V   G   +LHN L+   D
Sbjct: 307 EFNNVVHTGLHSVEIAFAKVRARWRILDKKWKPETIEFMPFVITTGC--LLHNFLVNSGD 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2U35.7e-2627.89Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q94K499.7e-2629.12Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0LBX68.7e-17996.87DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G202740 PE... [more]
A0A5D3CRB28.2e-17795.92Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C2M88.2e-17795.92uncharacterized protein LOC103496169 OS=Cucumis melo OX=3656 GN=LOC103496169 PE=... [more]
A0A6J1D7F11.7e-15084.01protein ALP1-like OS=Momordica charantia OX=3673 GN=LOC111017620 PE=3 SV=1[more]
A0A6J1FNZ22.2e-15082.76protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446927 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KGN57516.11.8e-17896.87hypothetical protein Csa_011580 [Cucumis sativus][more]
KAA0037135.11.7e-17695.92putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK13940.1 putative nucleas... [more]
XP_008456140.11.7e-17695.92PREDICTED: uncharacterized protein LOC103496169 [Cucumis melo][more]
XP_038880641.15.6e-15686.83protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1-like [Benincasa hispida][more]
XP_023536005.16.4e-15284.01protein ALP1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G55350.14.0e-2727.89PIF / Ping-Pong family of plant transposases [more]
AT3G63270.16.9e-2729.12CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT3G19120.15.3e-1925.40PIF / Ping-Pong family of plant transposases [more]
AT4G29780.15.0e-1725.42unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G72270.12.3e-1429.15CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR0217... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 122..262
e-value: 2.0E-12
score: 47.0
NoneNo IPR availablePANTHERPTHR22930:SF190OS06G0164500 PROTEINcoord: 1..318
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G002220.1Lsi05G002220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding