Lsi05G002190 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G002190 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 7.6e-151
Identity = 276/428 (64.49%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPG     +EPLDL + IPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCP
Sbjct: 1   MPGN----LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTR  L+DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPA+P  VRSLL QAS+ + 
Sbjct: 61  VTRVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITG 120

Query: 121 ---GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRE 180
                  R +A+RRL+GLARDS+KNR LI+  NAREIL+ ++F+  D ++ S+S +L  E
Sbjct: 121 THVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFA--DIETTSLSSELVSE 180

Query: 181 SLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELR 240
           SLALLV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAAALIE+V+ G ++ +L+
Sbjct: 181 SLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLK 240

Query: 241 SQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRF 300
             I G D +FEGV+++L++P +  RALK+G+KA+FALCLVKQTRH A+SAGA  I+IDR 
Sbjct: 241 LIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRL 300

Query: 301 -PDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360
             D ++ D ER LAT+EL+CR+P GC AF  HALTVPL+VK IL++SDRATE A GAL++
Sbjct: 301 AADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLA 360

Query: 361 LCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYA 420
           LC+A E  R EA AAG++TQLLLLVQSDCTER KRK+Q+LLKLLRDSWP +S  +SDD+ 
Sbjct: 361 LCTAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHSDDFN 420

Query: 421 YSEIVVPF 425
            SE V PF
Sbjct: 421 RSE-VAPF 421

BLAST of Lsi05G002190 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.1e-144
Identity = 271/423 (64.07%), Postives = 335/423 (79.20%), Query Frame = 0

Query: 9   VEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 68
           +EPLDL + IPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 69  DFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS---GSSL 128
           DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPA+P+ VR+LL QAS+ +        
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 129 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVM 188
           R +ALRRL+G ARDSDKNR LI++ NA EIL+ ++F      S++ S +L  ESLALLVM
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVM 184

Query: 189 LPLTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGI 248
           LP+TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIEIV  GT++++L+  I   
Sbjct: 185 LPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 249 DELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRF-PDLEK 308
           + +FEGV+++LR+P +  RALK+G+K LFALC VK TRH A++AGA EI+IDR   D ++
Sbjct: 245 ESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 304

Query: 309 YDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASE 368
            D ERALAT+EL+CR P GC AF  HALTVPLLVK IL++SDRATE A GAL++LC+A E
Sbjct: 305 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEE 364

Query: 369 ENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ-ESVGNSDDYAYSEIV 425
             R EA  AG++ QLLL+VQS+CTER K+K+Q LLKLLRDSWP   S  NSDD+  S  V
Sbjct: 365 RWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDFGCSSQV 421

BLAST of Lsi05G002190 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.6e-50
Identity = 135/403 (33.50%), Postives = 213/403 (52.85%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +TD  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 72  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSLRISALR 131
           L PNHTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+  ++S       ++  L+
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLK 125

Query: 132 RLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVMLPLTES 191
           RL+ +  ++  N+  + +    E L ++V       +DS +  L+ E+L LL  L  +E+
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETSET 185

Query: 192 ---ECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGIDELF 251
                +    D   +  L+ ++     E R+ A  L++ ++     ++    +    E+F
Sbjct: 186 VLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL---EVADPMQSMTLKPEVF 245

Query: 252 EGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPD----LEKY 311
             VV+IL D  +  +A K  +  L  +C   + RHKAV AG   +II+   D     E+ 
Sbjct: 246 TEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 305

Query: 312 DAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSL--CSAS 371
             E A+  ++L+C+   G   F  H   + ++ K IL++S  A++ AV  L+S+    A+
Sbjct: 306 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 365

Query: 372 EENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 405
                E +  G++ +L L++Q  C  + K K++ LLKL    W
Sbjct: 366 PALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Lsi05G002190 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.1e-48
Identity = 138/426 (32.39%), Postives = 222/426 (52.11%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +T+  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 72  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSLRISALR 131
           L PNHTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ ++SS       ++  L+
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLN---QVKCLK 120

Query: 132 RLKGLARDSDKNRSLISSLNAREILLDVVFSNLD---SDSDSVSP--------------- 191
           RL+ +  ++  N+  + +    E L ++V +++D   S S S+S                
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 192 -DLSRESL--ALLVMLPLTESECVLVA-----SDPQRIGYLSHLLFDSSIEVRINAAALI 251
            D SR  +  AL V+  L  SE  L +          +  L+ ++     E R  AA L+
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 252 EIVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 311
           + ++     ++    I    ELF  V++IL D  ++ +A +  ++ L   C   + RHKA
Sbjct: 241 KKLL---EVADPMQIILLERELFGEVIQILHDQISH-KATRSAMQILVITCPWGRNRHKA 300

Query: 312 VSAGAAEIIIDRFPD----LEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVIL 371
           V  G   +II+   D     E+ ++E A+  ++++C+   G   F  H   + ++ K IL
Sbjct: 301 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 360

Query: 372 KISDRATESAVGALVSL--CSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLK 405
           ++S   +E AV  L+S+    A+    +E +  G++ +L L++Q  C  + K K++ LLK
Sbjct: 361 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 419

BLAST of Lsi05G002190 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 4.0e-43
Identity = 125/391 (31.97%), Postives = 204/391 (52.17%), Query Frame = 0

Query: 16  VHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNH 75
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPNH
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNH 88

Query: 76  TLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSL---LGQASSRSSGSSLRISALRRL 135
           T+R++IQ WCV   S  ++RIPTP+ P  P  V  +   L  A+ R       +  + ++
Sbjct: 89  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGV-IIEKI 148

Query: 136 KGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVMLPLTESEC 195
           K L  +S+KNR  ++  +   +L D  F     D       +  E L+LL  +     E 
Sbjct: 149 KKLGDESEKNRKCVNENSVGWVLCD-CFDKFSGDEKLTF--MLNEILSLLTWMFPIGLEG 208

Query: 196 VLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGIDE-LFEGVV 255
           +   +       ++ LL  +   VR NAA +++ +++     E R     ++  + E +V
Sbjct: 209 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS---LDETRVHSFAVENGVAEALV 268

Query: 256 EILRDPTAYPRALKVGVKALFALCLVK-QTRHKAVSAGAAEIIIDRFPDLEKYDAERALA 315
           +++RD  +   + K  + A++ + L K +   + +  G   I ++   D E    E+ALA
Sbjct: 269 KLIRDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCEKALA 328

Query: 316 TIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEENR-REAV 375
            ++ IC    G +    +AL +PLLVK I K+S+ AT S++  ++ L          +AV
Sbjct: 329 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVEDAV 388

Query: 376 AAGILTQLLLLVQSDCTERVKRKSQVLLKLL 401
             G   ++LL++Q    E  K K+  LLK++
Sbjct: 389 RLGAFQKVLLVLQVGYGEETKEKATELLKMM 411

BLAST of Lsi05G002190 vs. ExPASy TrEMBL
Match: A0A5A7T4S1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001500 PE=4 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 7.9e-228
Identity = 416/425 (97.88%), Postives = 420/425 (98.82%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120

Query: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLA 180
           GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDS SDS SPDLSRESLA
Sbjct: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLA 180

Query: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQI 240
           LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR+QI
Sbjct: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRTQI 240

Query: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300
           CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL
Sbjct: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300

Query: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           EKYDAERALATIELICR+PTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA
Sbjct: 301 EKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360

Query: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEI 420
           SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDY  SEI
Sbjct: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEI 420

Query: 421 VVPFC 426
           VVPFC
Sbjct: 421 VVPFC 425

BLAST of Lsi05G002190 vs. ExPASy TrEMBL
Match: A0A1S3C380 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496167 PE=4 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 7.9e-228
Identity = 416/425 (97.88%), Postives = 420/425 (98.82%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120

Query: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLA 180
           GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDS SDS SPDLSRESLA
Sbjct: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLA 180

Query: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQI 240
           LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR+QI
Sbjct: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRTQI 240

Query: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300
           CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL
Sbjct: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300

Query: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           EKYDAERALATIELICR+PTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA
Sbjct: 301 EKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360

Query: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEI 420
           SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDY  SEI
Sbjct: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEI 420

Query: 421 VVPFC 426
           VVPFC
Sbjct: 421 VVPFC 425

BLAST of Lsi05G002190 vs. ExPASy TrEMBL
Match: A0A0A0L6F8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G203270 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.0e-226
Identity = 415/425 (97.65%), Postives = 419/425 (98.59%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTRAPLTDFTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPAEPSLVRSLL QASSRSS
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPAEPSLVRSLLAQASSRSS 120

Query: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLA 180
           GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDS SDS SPDLSRESLA
Sbjct: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLA 180

Query: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQI 240
           LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIE RINAAALIE VIAGTRASELR+QI
Sbjct: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEDRINAAALIETVIAGTRASELRTQI 240

Query: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300
           CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL
Sbjct: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300

Query: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA
Sbjct: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360

Query: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEI 420
           SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYA SEI
Sbjct: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYACSEI 420

Query: 421 VVPFC 426
           VVPFC
Sbjct: 421 VVPFC 425

BLAST of Lsi05G002190 vs. ExPASy TrEMBL
Match: A0A6J1IYF7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480329 PE=4 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.0e-215
Identity = 397/420 (94.52%), Postives = 409/420 (97.38%), Query Frame = 0

Query: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64
           GSV VEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62

Query: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124
           PLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLLGQASS+SSGSS 
Sbjct: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122

Query: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVM 184
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN  SDSDSVSPDLSRESLALLVM
Sbjct: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--SDSDSVSPDLSRESLALLVM 182

Query: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGID 244
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELR QIC ID
Sbjct: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242

Query: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302

Query: 305 AERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364
           AERALATIELICRIPTG DAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 303 AERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362

Query: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEIVVPF 424
           RREAVAAGI TQLLLLVQSDCT+RVKRKSQVLLKLLRDSWPQ+SVGNSDDY  SEIV+PF
Sbjct: 363 RREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Lsi05G002190 vs. ExPASy TrEMBL
Match: A0A6J1FSF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447739 PE=4 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.0e-215
Identity = 397/420 (94.52%), Postives = 409/420 (97.38%), Query Frame = 0

Query: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64
           GSV VEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62

Query: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124
           PLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLLGQASS+SSGSS 
Sbjct: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122

Query: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVM 184
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN  SDSDSVSPDLSRESLALLVM
Sbjct: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--SDSDSVSPDLSRESLALLVM 182

Query: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGID 244
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELR QIC ID
Sbjct: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242

Query: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302

Query: 305 AERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364
           AERALATIELICRIPTG DAFA+HALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 303 AERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362

Query: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEIVVPF 424
           RREAVAAGI TQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ+SVGNSDDY  SEIV+PF
Sbjct: 363 RREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Lsi05G002190 vs. NCBI nr
Match: XP_038875257.1 (U-box domain-containing protein 26-like isoform X2 [Benincasa hispida] >XP_038875267.1 U-box domain-containing protein 26-like isoform X3 [Benincasa hispida])

HSP 1 Score: 800.0 bits (2065), Expect = 9.6e-228
Identity = 418/425 (98.35%), Postives = 419/425 (98.59%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLL QASSRSS
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQASSRSS 120

Query: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLA 180
           G +LRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDS SPDLSRESLA
Sbjct: 121 GLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSFSPDLSRESLA 180

Query: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQI 240
           LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELRSQI
Sbjct: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRSQI 240

Query: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300
           CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL
Sbjct: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300

Query: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA
Sbjct: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360

Query: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEI 420
           SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDY  SEI
Sbjct: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEI 420

Query: 421 VVPFC 426
           VVPFC
Sbjct: 421 VVPFC 425

BLAST of Lsi05G002190 vs. NCBI nr
Match: XP_008456137.1 (PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0037126.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa] >TYK13948.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa])

HSP 1 Score: 799.3 bits (2063), Expect = 1.6e-227
Identity = 416/425 (97.88%), Postives = 420/425 (98.82%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120

Query: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLA 180
           GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDS SDS SPDLSRESLA
Sbjct: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLA 180

Query: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQI 240
           LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VIAGTRASELR+QI
Sbjct: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRTQI 240

Query: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300
           CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL
Sbjct: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300

Query: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           EKYDAERALATIELICR+PTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA
Sbjct: 301 EKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360

Query: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEI 420
           SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDY  SEI
Sbjct: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEI 420

Query: 421 VVPFC 426
           VVPFC
Sbjct: 421 VVPFC 425

BLAST of Lsi05G002190 vs. NCBI nr
Match: XP_004140703.1 (U-box domain-containing protein 26 [Cucumis sativus] >KGN57520.1 hypothetical protein Csa_011013 [Cucumis sativus])

HSP 1 Score: 794.7 bits (2051), Expect = 4.0e-226
Identity = 415/425 (97.65%), Postives = 419/425 (98.59%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTRAPLTDFTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPAEPSLVRSLL QASSRSS
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPAEPSLVRSLLAQASSRSS 120

Query: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLA 180
           GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDS SDS SPDLSRESLA
Sbjct: 121 GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSGSDSFSPDLSRESLA 180

Query: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQI 240
           LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIE RINAAALIE VIAGTRASELR+QI
Sbjct: 181 LLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEDRINAAALIETVIAGTRASELRTQI 240

Query: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300
           CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL
Sbjct: 241 CGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDL 300

Query: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360
           EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA
Sbjct: 301 EKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSA 360

Query: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEI 420
           SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYA SEI
Sbjct: 361 SEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYACSEI 420

Query: 421 VVPFC 426
           VVPFC
Sbjct: 421 VVPFC 425

BLAST of Lsi05G002190 vs. NCBI nr
Match: XP_038875248.1 (U-box domain-containing protein 26-like isoform X1 [Benincasa hispida])

HSP 1 Score: 789.6 bits (2038), Expect = 1.3e-224
Identity = 418/441 (94.78%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPN----------------HTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPN                HTLRRLIQDWCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLGQASSRSSGSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLL QASSRSSG +LRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSVSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           SDSDS SPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI
Sbjct: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240

Query: 241 EIVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           E VIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYAYSEIVVPFC 426
           PQESVGNSDDY  SEIVVPFC
Sbjct: 421 PQESVGNSDDYGCSEIVVPFC 441

BLAST of Lsi05G002190 vs. NCBI nr
Match: XP_022981075.1 (U-box domain-containing protein 26-like [Cucurbita maxima])

HSP 1 Score: 758.1 bits (1956), Expect = 4.2e-215
Identity = 397/420 (94.52%), Postives = 409/420 (97.38%), Query Frame = 0

Query: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64
           GSV VEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62

Query: 65  PLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSL 124
           PLT+FTLIPNHTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLVRSLLGQASS+SSGSS 
Sbjct: 63  PLTEFTLIPNHTLRRLIQEWCVANRSYGVERIPTPKQPADPSLVRSLLGQASSKSSGSSS 122

Query: 125 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVM 184
           RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN  SDSDSVSPDLSRESLALLVM
Sbjct: 123 RISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--SDSDSVSPDLSRESLALLVM 182

Query: 185 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGID 244
           LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELR QIC ID
Sbjct: 183 LPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRGQICAID 242

Query: 245 ELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYD 304
           ELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAGAAEI+IDRFPDLEKYD
Sbjct: 243 ELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAGAAEILIDRFPDLEKYD 302

Query: 305 AERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 364
           AERALATIELICRIPTG DAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN
Sbjct: 303 AERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEEN 362

Query: 365 RREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYAYSEIVVPF 424
           RREAVAAGI TQLLLLVQSDCT+RVKRKSQVLLKLLRDSWPQ+SVGNSDDY  SEIV+PF
Sbjct: 363 RREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDSVGNSDDYGCSEIVIPF 420

BLAST of Lsi05G002190 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 535.0 bits (1377), Expect = 5.4e-152
Identity = 276/428 (64.49%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPG     +EPLDL + IPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCP
Sbjct: 1   MPGN----LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS 120
           VTR  L+DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPA+P  VRSLL QAS+ + 
Sbjct: 61  VTRVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITG 120

Query: 121 ---GSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRE 180
                  R +A+RRL+GLARDS+KNR LI+  NAREIL+ ++F+  D ++ S+S +L  E
Sbjct: 121 THVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFA--DIETTSLSSELVSE 180

Query: 181 SLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELR 240
           SLALLV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAAALIE+V+ G ++ +L+
Sbjct: 181 SLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLK 240

Query: 241 SQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRF 300
             I G D +FEGV+++L++P +  RALK+G+KA+FALCLVKQTRH A+SAGA  I+IDR 
Sbjct: 241 LIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRL 300

Query: 301 -PDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVS 360
             D ++ D ER LAT+EL+CR+P GC AF  HALTVPL+VK IL++SDRATE A GAL++
Sbjct: 301 AADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLA 360

Query: 361 LCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYA 420
           LC+A E  R EA AAG++TQLLLLVQSDCTER KRK+Q+LLKLLRDSWP +S  +SDD+ 
Sbjct: 361 LCTAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHSDDFN 420

Query: 421 YSEIVVPF 425
            SE V PF
Sbjct: 421 RSE-VAPF 421

BLAST of Lsi05G002190 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 514.6 bits (1324), Expect = 7.5e-146
Identity = 271/423 (64.07%), Postives = 335/423 (79.20%), Query Frame = 0

Query: 9   VEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 68
           +EPLDL + IPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 69  DFTLIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSS---GSSL 128
           DFTLIPNHTLRRLIQ+WCVANRS GVERIPTPKQPA+P+ VR+LL QAS+ +        
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 129 RISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVM 188
           R +ALRRL+G ARDSDKNR LI++ NA EIL+ ++F      S++ S +L  ESLALLVM
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVM 184

Query: 189 LPLTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGI 248
           LP+TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIEIV  GT++++L+  I   
Sbjct: 185 LPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 249 DELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRF-PDLEK 308
           + +FEGV+++LR+P +  RALK+G+K LFALC VK TRH A++AGA EI+IDR   D ++
Sbjct: 245 ESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 304

Query: 309 YDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASE 368
            D ERALAT+EL+CR P GC AF  HALTVPLLVK IL++SDRATE A GAL++LC+A E
Sbjct: 305 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEE 364

Query: 369 ENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ-ESVGNSDDYAYSEIV 425
             R EA  AG++ QLLL+VQS+CTER K+K+Q LLKLLRDSWP   S  NSDD+  S  V
Sbjct: 365 RWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDFGCSSQV 421

BLAST of Lsi05G002190 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 201.1 bits (510), Expect = 1.8e-51
Identity = 135/403 (33.50%), Postives = 213/403 (52.85%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +TD  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 72  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSLRISALR 131
           L PNHTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+  ++S       ++  L+
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLK 125

Query: 132 RLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVMLPLTES 191
           RL+ +  ++  N+  + +    E L ++V       +DS +  L+ E+L LL  L  +E+
Sbjct: 126 RLRQIVSENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETSET 185

Query: 192 ---ECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGIDELF 251
                +    D   +  L+ ++     E R+ A  L++ ++     ++    +    E+F
Sbjct: 186 VLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL---EVADPMQSMTLKPEVF 245

Query: 252 EGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPD----LEKY 311
             VV+IL D  +  +A K  +  L  +C   + RHKAV AG   +II+   D     E+ 
Sbjct: 246 TEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 305

Query: 312 DAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSL--CSAS 371
             E A+  ++L+C+   G   F  H   + ++ K IL++S  A++ AV  L+S+    A+
Sbjct: 306 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 365

Query: 372 EENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 405
                E +  G++ +L L++Q  C  + K K++ LLKL    W
Sbjct: 366 PALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Lsi05G002190 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 193.7 bits (491), Expect = 2.9e-49
Identity = 138/426 (32.39%), Postives = 222/426 (52.11%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +T+  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 72  LIPNHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLGQASSRSSGSSLRISALR 131
           L PNHTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ ++SS       ++  L+
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLN---QVKCLK 120

Query: 132 RLKGLARDSDKNRSLISSLNAREILLDVVFSNLD---SDSDSVSP--------------- 191
           RL+ +  ++  N+  + +    E L ++V +++D   S S S+S                
Sbjct: 121 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 180

Query: 192 -DLSRESL--ALLVMLPLTESECVLVA-----SDPQRIGYLSHLLFDSSIEVRINAAALI 251
            D SR  +  AL V+  L  SE  L +          +  L+ ++     E R  AA L+
Sbjct: 181 FDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 240

Query: 252 EIVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 311
           + ++     ++    I    ELF  V++IL D  ++ +A +  ++ L   C   + RHKA
Sbjct: 241 KKLL---EVADPMQIILLERELFGEVIQILHDQISH-KATRSAMQILVITCPWGRNRHKA 300

Query: 312 VSAGAAEIIIDRFPD----LEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVIL 371
           V  G   +II+   D     E+ ++E A+  ++++C+   G   F  H   + ++ K IL
Sbjct: 301 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 360

Query: 372 KISDRATESAVGALVSL--CSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLK 405
           ++S   +E AV  L+S+    A+    +E +  G++ +L L++Q  C  + K K++ LLK
Sbjct: 361 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 419

BLAST of Lsi05G002190 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.8e-44
Identity = 125/391 (31.97%), Postives = 204/391 (52.17%), Query Frame = 0

Query: 16  VHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNH 75
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPNH
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNH 88

Query: 76  TLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSL---LGQASSRSSGSSLRISALRRL 135
           T+R++IQ WCV   S  ++RIPTP+ P  P  V  +   L  A+ R       +  + ++
Sbjct: 89  TIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGV-IIEKI 148

Query: 136 KGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSVSPDLSRESLALLVMLPLTESEC 195
           K L  +S+KNR  ++  +   +L D  F     D       +  E L+LL  +     E 
Sbjct: 149 KKLGDESEKNRKCVNENSVGWVLCD-CFDKFSGDEKLTF--MLNEILSLLTWMFPIGLEG 208

Query: 196 VLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVIAGTRASELRSQICGIDE-LFEGVV 255
           +   +       ++ LL  +   VR NAA +++ +++     E R     ++  + E +V
Sbjct: 209 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS---LDETRVHSFAVENGVAEALV 268

Query: 256 EILRDPTAYPRALKVGVKALFALCLVK-QTRHKAVSAGAAEIIIDRFPDLEKYDAERALA 315
           +++RD  +   + K  + A++ + L K +   + +  G   I ++   D E    E+ALA
Sbjct: 269 KLIRDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVCEKALA 328

Query: 316 TIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEENR-REAV 375
            ++ IC    G +    +AL +PLLVK I K+S+ AT S++  ++ L          +AV
Sbjct: 329 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVEDAV 388

Query: 376 AAGILTQLLLLVQSDCTERVKRKSQVLLKLL 401
             G   ++LL++Q    E  K K+  LLK++
Sbjct: 389 RLGAFQKVLLVLQVGYGEETKEKATELLKMM 411