Lsi05G002170 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G002170 vs. ExPASy TrEMBL
Match: A0A0A0L996 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G204780 PE=4 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 1.3e-200
Identity = 421/699 (60.23%), Postives = 480/699 (68.67%), Query Frame = 0

Query: 1   MATDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLT-ANRPFL 60
           MAT+K KEE RSRKPRHRRN QMEEFPTFTKWLT+FGHS S +DA SKS  L  ANRP  
Sbjct: 1   MATNKPKEEQRSRKPRHRRNQQMEEFPTFTKWLTTFGHSGSCNDAKSKSKQLNPANRPLQ 60

Query: 61  KTPVVVLDSSQHAAAVVADVPPKED-RVQSVDDSISAR--AACCCWQTSKSTRRECALKL 120
           + PVV+  SS+ A  VV +VP +E  +  +VD SISAR  A CCCWQ+SKSTRRECALK 
Sbjct: 61  RPPVVLPASSEDAVPVVTNVPEEEGVQTMAVDGSISARGGAGCCCWQSSKSTRRECALKF 120

Query: 121 HLSLRKRKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRRS 180
           H+SLRKRKVV N S++A VVAAV N  E+ TV  V     KDGCGCRC  TF+I  RR+S
Sbjct: 121 HISLRKRKVVAN-STEAEVVAAVLNPPEEATVEDV-----KDGCGCRCLRTFKIFRRRKS 180

Query: 181 TVVATSDLQKQEVA------------ATNGPDLVKEEEVVVVDPDLRKEEGSGCCCGGCG 240
            VV  SDLQK+E A            A++G D++KEEEVV+  PD RKEE SGCCCG   
Sbjct: 181 RVVGVSDLQKEEGAVTDGVNLRTEEVASSGSDMMKEEEVVIA-PDRRKEEESGCCCGRWK 240

Query: 241 LLPAFQICRRRKLVAVKEEVIVDVPKVEELANDDVTIQEGDSVGCLRAFHICAGRKRVDD 300
             P FQICRRRK+VA KEEV+   PKVEE+ ND+VT QE DSVGCL+AFHIC GRKRVDD
Sbjct: 241 CSPTFQICRRRKVVAGKEEVVGGAPKVEEVGNDNVTKQEEDSVGCLQAFHICGGRKRVDD 300

Query: 301 NPKTFEKKEAVATDDSSNVDIPDLQKERSGCCSCFKCVPTFQICGRRRRNVDSDVPNGCC 360
           NPKT E KE + ++DSSN+D+ +LQKE SGCCSCF+C+PTFQICG RR N D        
Sbjct: 301 NPKTSE-KEPLVSNDSSNLDVQNLQKEESGCCSCFRCIPTFQICGGRRSNED-------- 360

Query: 361 SCFKCAPSFHICGRRRRNVESDVPNPGREEKVVVGVSDLPEEEVVAADKERHSKSAQGGT 420
                               S VP PGREEKV+V VSD PE   V   +ERHS+  QGGT
Sbjct: 361 --------------------SGVPKPGREEKVIVDVSDPPEMGSVVDGRERHSRPVQGGT 420

Query: 421 CWPFQICARGWLPRFFLCGEKTAVDASNHREEEEKTPPDVGKEEEVVAVIPDPQKESIAV 480
           CW       GW PRF LCGE TAVDA NHREEEEK P D  KEE+VV         + AV
Sbjct: 421 CW------SGWFPRFLLCGEGTAVDAPNHREEEEKAPSDARKEEKVVV--------ATAV 480

Query: 481 ADGIPDHGKEKQMAADDIPVLSTEEMSAG--------KEKNVSSGSIQEVRKEEEIVDSG 540
            D I DH KEK +AA DIPV++ EE+  G        KEKNVSS +IQ+VRK EEIVDS 
Sbjct: 481 GDEISDHDKEKPVAAIDIPVVNEEEVFVGAGDTLDLHKEKNVSSCNIQDVRK-EEIVDSD 540

Query: 541 VSDLGEEEGGGCRCFKLGGKEGGRRQRRRSSRPREGGCAFQICGRGWLPTLHICRGRKKV 600
                + EGGGC C+   GKE G RQ+ RSSR  EG  +FQICGRG LPTL+ICRGRK V
Sbjct: 541 ----EKVEGGGCGCW---GKESGSRQQHRSSRSMEGCWSFQICGRGCLPTLNICRGRKDV 600

Query: 601 SVSIPKLHEEEGLVDNGVSDVHNEAVDAAGVTRVVAATDHPKSTRRCGCWHSKPRRRRAV 660
           SV I KL  EEGLVDN VSDVH E VDA GVT VVA +D+ K  R CGCW+ K RRRRAV
Sbjct: 601 SVRISKL-VEEGLVDNDVSDVHKEVVDATGVTDVVAGSDNSKCRRGCGCWNLKSRRRRAV 640

Query: 661 AVDKDGGSGRCSKSKCRR---GWLRRWGRKQREGKERNR 673
           AVDK+GGSGR SK K R+   GWLRR  RK+REGKE+NR
Sbjct: 661 AVDKEGGSGRRSKFKGRKGRGGWLRRSRRKEREGKEKNR 640

BLAST of Lsi05G002170 vs. ExPASy TrEMBL
Match: A0A6J1E9I0 (uncharacterized protein LOC111430613 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 9.0e-109
Identity = 348/905 (38.45%), Postives = 425/905 (46.96%), Query Frame = 0

Query: 3   TDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLKTP 62
           TDKQKE+ + +K  HRRNLQ+EEFPTFTKWL   G SSS  D +SKS       P+    
Sbjct: 4   TDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKS--YKRGVPYPPVR 63

Query: 63  VVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSLRK 122
            +  DSS     VVA    KED   ++ +SISAR  CCCWQ SKST+REC L  H SLRK
Sbjct: 64  RIPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLRK 123

Query: 123 RKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRR------- 182
            KVVTN S   GV A V ++RE+  VV  G V++++GCGCRC PTF ICGRR+       
Sbjct: 124 SKVVTNVSE--GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 183

Query: 183 -------------------STVVATSDLQKQEVAATNGPDLVKEEEVVVVDPDLRKE--- 242
                                VV   +L K+E  AT+GPD++K EEV    PDL KE   
Sbjct: 184 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLK-EEVATSCPDLVKEGEA 243

Query: 243 --------EGSGCCCGGCGLLPAFQICRRRKLVAVKEEVIVDV---------PKVEELAN 302
                   EGS  CC     LP+F +C  RKL  V+EEV ++          P+VEE+AN
Sbjct: 244 IAAPGPCKEGSRSCCSQWKCLPSFPMC-GRKLSVVEEEVTIEEEVSVYDPNGPEVEEVAN 303

Query: 303 DDVTIQEGDSV-----------------GCLRAFHICAGRK--------RVDD-NPKTFE 362
               ++EG++                   CL +F  C GRK         VDD N     
Sbjct: 304 GPELVKEGEATVAPGPCKEESGYCFPQWKCLPSFPTC-GRKLSVVEEEVTVDDPNVPEVA 363

Query: 363 KKEAVATDDSSNVDIPDLQKERSGCCS--------------------------------- 422
            +  +A +  + V  PD +KE SGCCS                                 
Sbjct: 364 NRPDLAKEGEATV-APDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNVLEV 423

Query: 423 --------------CFK-----CVPTFQICG----------------------------- 482
                         C +      +P FQIC                              
Sbjct: 424 EEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVANDVVK 483

Query: 483 RRRRNVDSD--------------------------VP----NGC-CSCFKCAPSFHICGR 542
           ++++ VD D                          VP    NG  C  FK  PSF ICG 
Sbjct: 484 KQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSGCGWFKWMPSFLICGS 543

Query: 543 RRRNVESDVPNPGREEKVVVGVSDLPEEEVVA-----------ADKERHSKSAQGGTCWP 602
           +   V SDVPNP REEKVV G SD+ E    A           AD E  SKSA+ G+CWP
Sbjct: 544 K---VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQSKSARQGSCWP 603

Query: 603 FQICARGWLPRFFLCGEKTAVDASNHREEEEKTPPDVGKEEEVVAVIPDPQKESIAVADG 662
           FQ C   W P   LC     V+ASNH+EEE++ P    K E+VV V+     E    A G
Sbjct: 604 FQCCTI-WWPSKLLCTGGMDVNASNHQEEEQEEPLSDNK-EKVVDVLTVKHNEK-GAAMG 663

Query: 663 IPDHGKE------KQMAADDIPVLSTEEM-SAG-------KEKNVSSGSIQEVRKEEEIV 673
           +PD  +E      KQ+A  DIPV   EE  SAG       KEK  SSG     ++EEE V
Sbjct: 664 VPDLHEEDGTVADKQVAVVDIPVPRKEEKESAGDTLDLIQKEKKASSG-----KEEEENV 723

BLAST of Lsi05G002170 vs. ExPASy TrEMBL
Match: A0A6J1E452 (uncharacterized protein LOC111430613 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 6.7e-104
Identity = 349/977 (35.72%), Postives = 430/977 (44.01%), Query Frame = 0

Query: 3   TDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLKTP 62
           TDKQKE+ + +K  HRRNLQ+EEFPTFTKWL   G SSS  D +SKS       P+    
Sbjct: 4   TDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKS--YKRGVPYPPVR 63

Query: 63  VVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSLRK 122
            +  DSS     VVA    KED   ++ +SISAR  CCCWQ SKST+REC L  H SLRK
Sbjct: 64  RIPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLRK 123

Query: 123 RKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRR------- 182
            KVVTN S   GV A V ++RE+  VV  G V++++GCGCRC PTF ICGRR+       
Sbjct: 124 SKVVTNVSE--GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 183

Query: 183 -------------------STVVATSDLQKQEVAATNGPDLVKEEEVVVVDPDLRKE--- 242
                                VV   +L K+E  AT+GPD++K EEV    PDL KE   
Sbjct: 184 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLK-EEVATSCPDLVKEGEA 243

Query: 243 --------EGSGCCCGGCGLLPAFQICRRRKLVAVKEEVIVDV---------PKVEELAN 302
                   EGS  CC     LP+F +C  RKL  V+EEV ++          P+VEE+AN
Sbjct: 244 IAAPGPCKEGSRSCCSQWKCLPSFPMC-GRKLSVVEEEVTIEEEVSVYDPNGPEVEEVAN 303

Query: 303 DDVTIQEGDS----VGCLRAFHICAGRK------------------------------RV 362
              +++EG++      C      C+  K                               V
Sbjct: 304 GPESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEV 363

Query: 363 DDNPKTFEKKEA-----------------------------VATD--DSSNVDIPDL--- 422
            + P+  ++ EA                             VAT   + + VDIP+L   
Sbjct: 364 ANGPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKVELTVVDIPNLLEV 423

Query: 423 ---------------------QKERSG-CCSCFKCVPTFQICGRRRRNVDSDV----PN- 482
                                 K+RSG CCS +KC+P+F  CGR+   V+ +V    PN 
Sbjct: 424 EEVANGPELVKEGVATGSPGPYKKRSGSCCSQWKCLPSFPTCGRKLSVVEEEVTVDDPNV 483

Query: 483 --------------------------GCCS------------------------------ 542
                                     GCCS                              
Sbjct: 484 PEVANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNV 543

Query: 543 -----------------------------------------------------------C 602
                                                                       
Sbjct: 544 LEVEEVANDVVNKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSGCGW 603

Query: 603 FKCAPSFHICGRRRRNVESDVPNPGREEKVVVGVSDLPEEEVVA-----------ADKER 662
           FK  PSF ICG +   V SDVPNP REEKVV G SD+ E    A           AD E 
Sbjct: 604 FKWMPSFLICGSK---VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEA 663

Query: 663 HSKSAQGGTCWPFQICARGWLPRFFLCGEKTAVDASNHREEEEKTPPDVGKEEEVVAVIP 673
            SKSA+ G+CWPFQ C   W P   LC     V+ASNH+EEE++ P    K E+VV V+ 
Sbjct: 664 QSKSARQGSCWPFQCCTI-WWPSKLLCTGGMDVNASNHQEEEQEEPLSDNK-EKVVDVLT 723

BLAST of Lsi05G002170 vs. ExPASy TrEMBL
Match: A0A6J1E412 (uncharacterized protein LOC111430613 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 5.5e-98
Identity = 349/1028 (33.95%), Postives = 430/1028 (41.83%), Query Frame = 0

Query: 3    TDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLKTP 62
            TDKQKE+ + +K  HRRNLQ+EEFPTFTKWL   G SSS  D +SKS       P+    
Sbjct: 4    TDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKS--YKRGVPYPPVR 63

Query: 63   VVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSLRK 122
             +  DSS     VVA    KED   ++ +SISAR  CCCWQ SKST+REC L  H SLRK
Sbjct: 64   RIPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLRK 123

Query: 123  RKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRR------- 182
             KVVTN S   GV A V ++RE+  VV  G V++++GCGCRC PTF ICGRR+       
Sbjct: 124  SKVVTNVSE--GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 183

Query: 183  -------------------STVVATSDLQKQEVAATNGPDLVKEEEVVVVDPDLRKE--- 242
                                 VV   +L K+E  AT+GPD++K EEV    PDL KE   
Sbjct: 184  YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLK-EEVATSCPDLVKEGEA 243

Query: 243  --------EGSGCCCGGCGLLPAFQICRRRKLVAVKEEVIVDV---------PKVEELAN 302
                    EGS  CC     LP+F +C  RKL  V+EEV ++          P+VEE+AN
Sbjct: 244  IAAPGPCKEGSRSCCSQWKCLPSFPMC-GRKLSVVEEEVTIEEEVSVYDPNGPEVEEVAN 303

Query: 303  DDVTIQEGDS----VGCLRAFHICAGRK------------------------------RV 362
               +++EG++      C      C+  K                               V
Sbjct: 304  GPESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEV 363

Query: 363  DDNPKTFEKKEA-----------------------------VATD--DSSNVDIPDL--- 422
             + P+  ++ EA                             VAT   + + VDIP+L   
Sbjct: 364  ANGPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKVELTVVDIPNLLEV 423

Query: 423  ---------------------QKERSG-CCSCFKCVPTFQICGRRRRNVDSDV----PN- 482
                                  K+RSG CCS +KC+P+F  CGR+   V+ +V    PN 
Sbjct: 424  EEVANGPELVKEGVATGSPGPYKKRSGSCCSQWKCLPSFPTCGRKLSVVEEEVTVDDPNV 483

Query: 483  --------------------------GCCS------------------------------ 542
                                      GCCS                              
Sbjct: 484  PEVANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNV 543

Query: 543  ------------------------------------------------------------ 602
                                                                        
Sbjct: 544  LEVEEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVAND 603

Query: 603  --------------------------------------------------CFKCAPSFHI 662
                                                               FK  PSF I
Sbjct: 604  VVKKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSGCGWFKWMPSFLI 663

Query: 663  CGRRRRNVESDVPNPGREEKVVVGVSDLPEEEVVA-----------ADKERHSKSAQGGT 673
            CG +   V SDVPNP REEKVV G SD+ E    A           AD E  SKSA+ G+
Sbjct: 664  CGSK---VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQSKSARQGS 723

BLAST of Lsi05G002170 vs. ExPASy TrEMBL
Match: A0A6J1E7K5 (uncharacterized protein LOC111430613 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 1.6e-97
Identity = 343/1026 (33.43%), Postives = 421/1026 (41.03%), Query Frame = 0

Query: 3    TDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLKTP 62
            TDKQKE+ + +K  HRRNLQ+EEFPTFTKWL   G SSS  D +SKS       P+    
Sbjct: 4    TDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKS--YKRGVPYPPVR 63

Query: 63   VVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSLRK 122
             +  DSS     VVA    KED   ++ +SISAR  CCCWQ SKST+REC L  H SLRK
Sbjct: 64   RIPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLRK 123

Query: 123  RKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRR------- 182
             KVVTN S   GV A V ++RE+  VV  G V++++GCGCRC PTF ICGRR+       
Sbjct: 124  SKVVTNVSE--GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 183

Query: 183  -------------------STVVATSDLQKQEVAATNGPDLVKEEEVVVVDPDLRKE--- 242
                                 VV   +L K+E  AT+GPD++K EEV    PDL KE   
Sbjct: 184  YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLK-EEVATSCPDLVKEGEA 243

Query: 243  --------EGSGCCCGGCGLLPAFQICRRRKLVAVKEEVIVDV---------PKVEELAN 302
                    EGS  CC     LP+F +C  RKL  V+EEV ++          P+VEE+AN
Sbjct: 244  IAAPGPCKEGSRSCCSQWKCLPSFPMC-GRKLSVVEEEVTIEEEVSVYDPNGPEVEEVAN 303

Query: 303  DDVTIQEGDS----VGCLRAFHICAGRKRVDDNPKTFEKKEAVATDDSSNVDIPDLQ--- 362
               +++EG++      C      C+  K     P   E+K A   ++ + V++P+L    
Sbjct: 304  GPESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMC-ERKVATGEEELTVVNVPNLLDVE 363

Query: 363  --------------------KERSGCCSCFKCVPTFQICGR-----RRRNVDSDVPN--- 422
                                K    CCS +KC+P F +CGR     +    D DVPN   
Sbjct: 364  VANGPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKEELTDVDVPNLLE 423

Query: 423  --------------------GCCS-----CF---KCAPSFHICGRRRRNVE--------- 482
                                G C      CF   KC PSF  CGR+   VE         
Sbjct: 424  VEEVANGPELVKEGEATVAPGPCKEESGYCFPQWKCLPSFPTCGRKLSVVEEEVTVDDPN 483

Query: 483  ------------------------------------------------------------ 542
                                                                        
Sbjct: 484  VPEVANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPN 543

Query: 543  ------------------------------------------------------------ 602
                                                                        
Sbjct: 544  VLEVEEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVAN 603

Query: 603  ------------------------------------------------------------ 662
                                                                        
Sbjct: 604  DVVKKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSGCGWFKWMPSFL 663

Query: 663  -------SDVPNPGREEKVVVGVSDLPEEEVVA-----------ADKERHSKSAQGGTCW 673
                   SDVPNP REEKVV G SD+ E    A           AD E  SKSA+ G+CW
Sbjct: 664  ICGSKVVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQSKSARQGSCW 723

BLAST of Lsi05G002170 vs. NCBI nr
Match: XP_038880648.1 (uncharacterized protein LOC120072275 [Benincasa hispida])

HSP 1 Score: 904.8 bits (2337), Expect = 4.4e-259
Identity = 499/689 (72.42%), Postives = 539/689 (78.23%), Query Frame = 0

Query: 1   MATDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLK 60
           M TDKQ EE +SRKPR RRNLQMEE PTFTKWL S GHS S +DANSKS  +  NRP ++
Sbjct: 6   MTTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVR 65

Query: 61  TPVVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSL 120
           TPVVV DSS+   AVVADVP  +DRVQ+VDDSISARA CCCWQ+SKSTRRECALK HLSL
Sbjct: 66  TPVVVFDSSEDGGAVVADVP--KDRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSL 125

Query: 121 RKRKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRRSTVV- 180
           RKRKVVTN  S+  VV  V NLRE++TVVAV DVL+KDGCGCRC+PTFQICGRR+S VV 
Sbjct: 126 RKRKVVTN-GSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRKSIVVL 185

Query: 181 ------ATSD--LQKQEVAATNGPDLVKEEEVVVV-DPDLRKEEGSGCCCGGCGLLPAFQ 240
                  T D  L+ +EV A  GPDLVKEEEVVVV   DLRKEEGSGCCCG CG  PAFQ
Sbjct: 186 QKEDGAVTDDPNLRTEEV-ANRGPDLVKEEEVVVVGGSDLRKEEGSGCCCGRCGCFPAFQ 245

Query: 241 ICRRRKLVAVKEEVIVDVPKV-EELANDDVTIQEGDSVGCLRAF--HI-CAGRKRVDDNP 300
           ICRRR +VA KEE +VDVP+V EE+AND V  QEGDSV CL+AF  HI CAGRK VDDNP
Sbjct: 246 ICRRRNVVASKEEAVVDVPEVEEEVANDKVNKQEGDSVECLQAFQSHICCAGRKTVDDNP 305

Query: 301 KTFEKKEAVATDDSSNVDIPDLQKERSGCCSCFKCVPTFQICGRRRRNVDSDVPNGCCSC 360
           KTFE KEA+A++DSSNVD+PDLQKE SG                           GCCSC
Sbjct: 306 KTFE-KEALASEDSSNVDVPDLQKEGSG---------------------------GCCSC 365

Query: 361 FKCAPSFHICGRRRRNVESDVPNPGREEKVVVGVSDLPE-EEVVAADKERHSKSAQGGTC 420
           FKC P+ HICG RRRN  S+VPNPGREEKVVV VSD PE EEVVAAD+E HSKS QGG C
Sbjct: 366 FKCMPTLHICG-RRRNAVSEVPNPGREEKVVVSVSDPPEGEEVVAADEEWHSKSTQGGIC 425

Query: 421 WPFQICARGWLPRFFLCGEKTAVDASNHREEEEKTPPDVGKEEEVVAVIPDPQKESIAVA 480
           WPFQIC RGWLPRFFLCGE+  VDASNHREEEEK PPDV K EEVVAVIPDPQKESIAVA
Sbjct: 426 WPFQICTRGWLPRFFLCGERITVDASNHREEEEKAPPDVQK-EEVVAVIPDPQKESIAVA 485

Query: 481 DGIPDHGKEKQMAADDIPVLSTEE-MSAGKEKNVSSGSIQEVRKEEEIVDSGVSDLG-EE 540
           DGIPD GKEKQ+AADDIPV STEE MSAGKE+N SSGSIQE  +          DL  E+
Sbjct: 486 DGIPDDGKEKQVAADDIPVQSTEEKMSAGKEENASSGSIQETFE---------PDLNQED 545

Query: 541 EGGGCRCFKLGGKEGGRRQRRRSSRPREGGCAFQICGRGWLPTLHICRGRKKVSVSIPKL 600
           EGG CRCFKLGGKEG RRQ RRS + REGG  FQIC  GWLPTL +CRGRKK SVSI KL
Sbjct: 546 EGGCCRCFKLGGKEGSRRQHRRSPKSREGGWGFQIC--GWLPTLDMCRGRKKESVSIAKL 605

Query: 601 HEEEGLVDNGVSDVHNEAVDAAGVTRVVAATDHPKSTRRCGCWHSKPRRRRAVAVDKDGG 660
           HE+EGLVDNGVS+VHNE VDAAGVT VVA TDH  STR CGCWHSKPRRRRAV V KDGG
Sbjct: 606 HEKEGLVDNGVSEVHNEVVDAAGVTGVVADTDHSNSTRCCGCWHSKPRRRRAVDVVKDGG 649

Query: 661 SGRCSKSKCRRGWLRRWGRKQREGKERNR 673
           SGRCSKSK R+GWLRRWGRKQREGKER +
Sbjct: 666 SGRCSKSKRRKGWLRRWGRKQREGKERKK 649

BLAST of Lsi05G002170 vs. NCBI nr
Match: KGN57524.1 (hypothetical protein Csa_011487 [Cucumis sativus])

HSP 1 Score: 709.5 bits (1830), Expect = 2.7e-200
Identity = 421/699 (60.23%), Postives = 480/699 (68.67%), Query Frame = 0

Query: 1   MATDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLT-ANRPFL 60
           MAT+K KEE RSRKPRHRRN QMEEFPTFTKWLT+FGHS S +DA SKS  L  ANRP  
Sbjct: 1   MATNKPKEEQRSRKPRHRRNQQMEEFPTFTKWLTTFGHSGSCNDAKSKSKQLNPANRPLQ 60

Query: 61  KTPVVVLDSSQHAAAVVADVPPKED-RVQSVDDSISAR--AACCCWQTSKSTRRECALKL 120
           + PVV+  SS+ A  VV +VP +E  +  +VD SISAR  A CCCWQ+SKSTRRECALK 
Sbjct: 61  RPPVVLPASSEDAVPVVTNVPEEEGVQTMAVDGSISARGGAGCCCWQSSKSTRRECALKF 120

Query: 121 HLSLRKRKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRRS 180
           H+SLRKRKVV N S++A VVAAV N  E+ TV  V     KDGCGCRC  TF+I  RR+S
Sbjct: 121 HISLRKRKVVAN-STEAEVVAAVLNPPEEATVEDV-----KDGCGCRCLRTFKIFRRRKS 180

Query: 181 TVVATSDLQKQEVA------------ATNGPDLVKEEEVVVVDPDLRKEEGSGCCCGGCG 240
            VV  SDLQK+E A            A++G D++KEEEVV+  PD RKEE SGCCCG   
Sbjct: 181 RVVGVSDLQKEEGAVTDGVNLRTEEVASSGSDMMKEEEVVIA-PDRRKEEESGCCCGRWK 240

Query: 241 LLPAFQICRRRKLVAVKEEVIVDVPKVEELANDDVTIQEGDSVGCLRAFHICAGRKRVDD 300
             P FQICRRRK+VA KEEV+   PKVEE+ ND+VT QE DSVGCL+AFHIC GRKRVDD
Sbjct: 241 CSPTFQICRRRKVVAGKEEVVGGAPKVEEVGNDNVTKQEEDSVGCLQAFHICGGRKRVDD 300

Query: 301 NPKTFEKKEAVATDDSSNVDIPDLQKERSGCCSCFKCVPTFQICGRRRRNVDSDVPNGCC 360
           NPKT E KE + ++DSSN+D+ +LQKE SGCCSCF+C+PTFQICG RR N D        
Sbjct: 301 NPKTSE-KEPLVSNDSSNLDVQNLQKEESGCCSCFRCIPTFQICGGRRSNED-------- 360

Query: 361 SCFKCAPSFHICGRRRRNVESDVPNPGREEKVVVGVSDLPEEEVVAADKERHSKSAQGGT 420
                               S VP PGREEKV+V VSD PE   V   +ERHS+  QGGT
Sbjct: 361 --------------------SGVPKPGREEKVIVDVSDPPEMGSVVDGRERHSRPVQGGT 420

Query: 421 CWPFQICARGWLPRFFLCGEKTAVDASNHREEEEKTPPDVGKEEEVVAVIPDPQKESIAV 480
           CW       GW PRF LCGE TAVDA NHREEEEK P D  KEE+VV         + AV
Sbjct: 421 CW------SGWFPRFLLCGEGTAVDAPNHREEEEKAPSDARKEEKVVV--------ATAV 480

Query: 481 ADGIPDHGKEKQMAADDIPVLSTEEMSAG--------KEKNVSSGSIQEVRKEEEIVDSG 540
            D I DH KEK +AA DIPV++ EE+  G        KEKNVSS +IQ+VRK EEIVDS 
Sbjct: 481 GDEISDHDKEKPVAAIDIPVVNEEEVFVGAGDTLDLHKEKNVSSCNIQDVRK-EEIVDSD 540

Query: 541 VSDLGEEEGGGCRCFKLGGKEGGRRQRRRSSRPREGGCAFQICGRGWLPTLHICRGRKKV 600
                + EGGGC C+   GKE G RQ+ RSSR  EG  +FQICGRG LPTL+ICRGRK V
Sbjct: 541 ----EKVEGGGCGCW---GKESGSRQQHRSSRSMEGCWSFQICGRGCLPTLNICRGRKDV 600

Query: 601 SVSIPKLHEEEGLVDNGVSDVHNEAVDAAGVTRVVAATDHPKSTRRCGCWHSKPRRRRAV 660
           SV I KL  EEGLVDN VSDVH E VDA GVT VVA +D+ K  R CGCW+ K RRRRAV
Sbjct: 601 SVRISKL-VEEGLVDNDVSDVHKEVVDATGVTDVVAGSDNSKCRRGCGCWNLKSRRRRAV 640

Query: 661 AVDKDGGSGRCSKSKCRR---GWLRRWGRKQREGKERNR 673
           AVDK+GGSGR SK K R+   GWLRR  RK+REGKE+NR
Sbjct: 661 AVDKEGGSGRRSKFKGRKGRGGWLRRSRRKEREGKEKNR 640

BLAST of Lsi05G002170 vs. NCBI nr
Match: XP_022922695.1 (uncharacterized protein LOC111430613 isoform X4 [Cucurbita moschata])

HSP 1 Score: 404.4 bits (1038), Expect = 1.9e-108
Identity = 348/905 (38.45%), Postives = 425/905 (46.96%), Query Frame = 0

Query: 3   TDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLKTP 62
           TDKQKE+ + +K  HRRNLQ+EEFPTFTKWL   G SSS  D +SKS       P+    
Sbjct: 4   TDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKS--YKRGVPYPPVR 63

Query: 63  VVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSLRK 122
            +  DSS     VVA    KED   ++ +SISAR  CCCWQ SKST+REC L  H SLRK
Sbjct: 64  RIPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLRK 123

Query: 123 RKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRR------- 182
            KVVTN S   GV A V ++RE+  VV  G V++++GCGCRC PTF ICGRR+       
Sbjct: 124 SKVVTNVSE--GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 183

Query: 183 -------------------STVVATSDLQKQEVAATNGPDLVKEEEVVVVDPDLRKE--- 242
                                VV   +L K+E  AT+GPD++K EEV    PDL KE   
Sbjct: 184 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLK-EEVATSCPDLVKEGEA 243

Query: 243 --------EGSGCCCGGCGLLPAFQICRRRKLVAVKEEVIVDV---------PKVEELAN 302
                   EGS  CC     LP+F +C  RKL  V+EEV ++          P+VEE+AN
Sbjct: 244 IAAPGPCKEGSRSCCSQWKCLPSFPMC-GRKLSVVEEEVTIEEEVSVYDPNGPEVEEVAN 303

Query: 303 DDVTIQEGDSV-----------------GCLRAFHICAGRK--------RVDD-NPKTFE 362
               ++EG++                   CL +F  C GRK         VDD N     
Sbjct: 304 GPELVKEGEATVAPGPCKEESGYCFPQWKCLPSFPTC-GRKLSVVEEEVTVDDPNVPEVA 363

Query: 363 KKEAVATDDSSNVDIPDLQKERSGCCS--------------------------------- 422
            +  +A +  + V  PD +KE SGCCS                                 
Sbjct: 364 NRPDLAKEGEATV-APDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNVLEV 423

Query: 423 --------------CFK-----CVPTFQICG----------------------------- 482
                         C +      +P FQIC                              
Sbjct: 424 EEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVANDVVK 483

Query: 483 RRRRNVDSD--------------------------VP----NGC-CSCFKCAPSFHICGR 542
           ++++ VD D                          VP    NG  C  FK  PSF ICG 
Sbjct: 484 KQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSGCGWFKWMPSFLICGS 543

Query: 543 RRRNVESDVPNPGREEKVVVGVSDLPEEEVVA-----------ADKERHSKSAQGGTCWP 602
           +   V SDVPNP REEKVV G SD+ E    A           AD E  SKSA+ G+CWP
Sbjct: 544 K---VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQSKSARQGSCWP 603

Query: 603 FQICARGWLPRFFLCGEKTAVDASNHREEEEKTPPDVGKEEEVVAVIPDPQKESIAVADG 662
           FQ C   W P   LC     V+ASNH+EEE++ P    K E+VV V+     E    A G
Sbjct: 604 FQCCTI-WWPSKLLCTGGMDVNASNHQEEEQEEPLSDNK-EKVVDVLTVKHNEK-GAAMG 663

Query: 663 IPDHGKE------KQMAADDIPVLSTEEM-SAG-------KEKNVSSGSIQEVRKEEEIV 673
           +PD  +E      KQ+A  DIPV   EE  SAG       KEK  SSG     ++EEE V
Sbjct: 664 VPDLHEEDGTVADKQVAVVDIPVPRKEEKESAGDTLDLIQKEKKASSG-----KEEEENV 723

BLAST of Lsi05G002170 vs. NCBI nr
Match: XP_022922694.1 (uncharacterized protein LOC111430613 isoform X3 [Cucurbita moschata])

HSP 1 Score: 388.3 bits (996), Expect = 1.4e-103
Identity = 349/977 (35.72%), Postives = 430/977 (44.01%), Query Frame = 0

Query: 3   TDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLKTP 62
           TDKQKE+ + +K  HRRNLQ+EEFPTFTKWL   G SSS  D +SKS       P+    
Sbjct: 4   TDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKS--YKRGVPYPPVR 63

Query: 63  VVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSLRK 122
            +  DSS     VVA    KED   ++ +SISAR  CCCWQ SKST+REC L  H SLRK
Sbjct: 64  RIPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLRK 123

Query: 123 RKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRR------- 182
            KVVTN S   GV A V ++RE+  VV  G V++++GCGCRC PTF ICGRR+       
Sbjct: 124 SKVVTNVSE--GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 183

Query: 183 -------------------STVVATSDLQKQEVAATNGPDLVKEEEVVVVDPDLRKE--- 242
                                VV   +L K+E  AT+GPD++K EEV    PDL KE   
Sbjct: 184 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLK-EEVATSCPDLVKEGEA 243

Query: 243 --------EGSGCCCGGCGLLPAFQICRRRKLVAVKEEVIVDV---------PKVEELAN 302
                   EGS  CC     LP+F +C  RKL  V+EEV ++          P+VEE+AN
Sbjct: 244 IAAPGPCKEGSRSCCSQWKCLPSFPMC-GRKLSVVEEEVTIEEEVSVYDPNGPEVEEVAN 303

Query: 303 DDVTIQEGDS----VGCLRAFHICAGRK------------------------------RV 362
              +++EG++      C      C+  K                               V
Sbjct: 304 GPESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEV 363

Query: 363 DDNPKTFEKKEA-----------------------------VATD--DSSNVDIPDL--- 422
            + P+  ++ EA                             VAT   + + VDIP+L   
Sbjct: 364 ANGPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKVELTVVDIPNLLEV 423

Query: 423 ---------------------QKERSG-CCSCFKCVPTFQICGRRRRNVDSDV----PN- 482
                                 K+RSG CCS +KC+P+F  CGR+   V+ +V    PN 
Sbjct: 424 EEVANGPELVKEGVATGSPGPYKKRSGSCCSQWKCLPSFPTCGRKLSVVEEEVTVDDPNV 483

Query: 483 --------------------------GCCS------------------------------ 542
                                     GCCS                              
Sbjct: 484 PEVANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNV 543

Query: 543 -----------------------------------------------------------C 602
                                                                       
Sbjct: 544 LEVEEVANDVVNKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSGCGW 603

Query: 603 FKCAPSFHICGRRRRNVESDVPNPGREEKVVVGVSDLPEEEVVA-----------ADKER 662
           FK  PSF ICG +   V SDVPNP REEKVV G SD+ E    A           AD E 
Sbjct: 604 FKWMPSFLICGSK---VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEA 663

Query: 663 HSKSAQGGTCWPFQICARGWLPRFFLCGEKTAVDASNHREEEEKTPPDVGKEEEVVAVIP 673
            SKSA+ G+CWPFQ C   W P   LC     V+ASNH+EEE++ P    K E+VV V+ 
Sbjct: 664 QSKSARQGSCWPFQCCTI-WWPSKLLCTGGMDVNASNHQEEEQEEPLSDNK-EKVVDVLT 723

BLAST of Lsi05G002170 vs. NCBI nr
Match: XP_022922692.1 (uncharacterized protein LOC111430613 isoform X1 [Cucurbita moschata])

HSP 1 Score: 368.6 bits (945), Expect = 1.1e-97
Identity = 349/1028 (33.95%), Postives = 430/1028 (41.83%), Query Frame = 0

Query: 3    TDKQKEEARSRKPRHRRNLQMEEFPTFTKWLTSFGHSSSTHDANSKSNLLTANRPFLKTP 62
            TDKQKE+ + +K  HRRNLQ+EEFPTFTKWL   G SSS  D +SKS       P+    
Sbjct: 4    TDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKS--YKRGVPYPPVR 63

Query: 63   VVVLDSSQHAAAVVADVPPKEDRVQSVDDSISARAACCCWQTSKSTRRECALKLHLSLRK 122
             +  DSS     VVA    KED   ++ +SISAR  CCCWQ SKST+REC L  H SLRK
Sbjct: 64   RIPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLRK 123

Query: 123  RKVVTNASSDAGVVAAVPNLREDDTVVAVGDVLDKDGCGCRCWPTFQICGRRR------- 182
             KVVTN S   GV A V ++RE+  VV  G V++++GCGCRC PTF ICGRR+       
Sbjct: 124  SKVVTNVSE--GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 183

Query: 183  -------------------STVVATSDLQKQEVAATNGPDLVKEEEVVVVDPDLRKE--- 242
                                 VV   +L K+E  AT+GPD++K EEV    PDL KE   
Sbjct: 184  YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLK-EEVATSCPDLVKEGEA 243

Query: 243  --------EGSGCCCGGCGLLPAFQICRRRKLVAVKEEVIVDV---------PKVEELAN 302
                    EGS  CC     LP+F +C  RKL  V+EEV ++          P+VEE+AN
Sbjct: 244  IAAPGPCKEGSRSCCSQWKCLPSFPMC-GRKLSVVEEEVTIEEEVSVYDPNGPEVEEVAN 303

Query: 303  DDVTIQEGDS----VGCLRAFHICAGRK------------------------------RV 362
               +++EG++      C      C+  K                               V
Sbjct: 304  GPESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEV 363

Query: 363  DDNPKTFEKKEA-----------------------------VATD--DSSNVDIPDL--- 422
             + P+  ++ EA                             VAT   + + VDIP+L   
Sbjct: 364  ANGPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKVELTVVDIPNLLEV 423

Query: 423  ---------------------QKERSG-CCSCFKCVPTFQICGRRRRNVDSDV----PN- 482
                                  K+RSG CCS +KC+P+F  CGR+   V+ +V    PN 
Sbjct: 424  EEVANGPELVKEGVATGSPGPYKKRSGSCCSQWKCLPSFPTCGRKLSVVEEEVTVDDPNV 483

Query: 483  --------------------------GCCS------------------------------ 542
                                      GCCS                              
Sbjct: 484  PEVANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNV 543

Query: 543  ------------------------------------------------------------ 602
                                                                        
Sbjct: 544  LEVEEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVAND 603

Query: 603  --------------------------------------------------CFKCAPSFHI 662
                                                               FK  PSF I
Sbjct: 604  VVKKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSGCGWFKWMPSFLI 663

Query: 663  CGRRRRNVESDVPNPGREEKVVVGVSDLPEEEVVA-----------ADKERHSKSAQGGT 673
            CG +   V SDVPNP REEKVV G SD+ E    A           AD E  SKSA+ G+
Sbjct: 664  CGSK---VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQSKSARQGS 723