Lsi05G001820 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G001820
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Nigrin b-like
Location	chr05: 2626318 .. 2627196 (+)
RNA-Seq Expression	Lsi05G001820
Synteny	Lsi05G001820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGAAATTGTGTTGAGCATTGTTGTGGCTTTCTCACTCACCACCCACCTTGTCATAGCATCGTCCTCAAATTATGGATATGGAGGATTCCCTGGAAGCACACATCTTGTAGGTCGAGATGGATTATGTTTAGAGGCATCTCCATGGTTTACCAAAGGTAATTATTTTTCAACTCGATTATCACCATGCGACGACGAACGTAGGATGAAACCAGCTCAATTATGGACAATCCTCCAAGACGGGACGATTCGAACGATGAATAATAGATATTGTTTGGTTTCTTACCTCCCTCTTGGTATGACCACAACCAATGTGATAGTAAGCTTATGTGAAAAGGAATCAAATCCTAACAAAATATGGAACCACAAGAAAGACGGGACCATAGTCCACGTCGAGTCCGGGATGGTTCTAACAGGAAAATTCGATAGCTATGTGACATTAGAAAGAAACGAAAATGTACCATCACAAAGCTGGGAAGTCACAGAAAATTTAACCCCAATGGTGGCGAACATCAAATGGCTTGACAACTTGTGTTTGCAATCTACGGAAGATTCGAATAATGTGGGGTTGGATGGATGTAATAGAGAAAATAAGAATCAAAGATGGGCATTATATGGAGATGGAACTATTCGACAAAATGTCAATAGAAACTATTGCTTGACTTCTAAAGAAGATTATGGTCATTATATAGCTGTGTCTAAATGTGAAGACATACCACAACAACGTTGGGGGCTTAGTGCAGAAGACAACACTATTAACCATCCAGCCAACATTGATATGGTCTTGGATGTGCTTGATGTGCCATTAAAGTATCCACTAGCGATCTTGATGAGCCATCGTGATGGAAGTCCTAGCCAGCGATGGATTATTTACTGA

mRNA sequence

ATGAGAGAAATTGTGTTGAGCATTGTTGTGGCTTTCTCACTCACCACCCACCTTGTCATAGCATCGTCCTCAAATTATGGATATGGAGGATTCCCTGGAAGCACACATCTTGTAGGTCGAGATGGATTATGTTTAGAGGCATCTCCATGGTTTACCAAAGGTAATTATTTTTCAACTCGATTATCACCATGCGACGACGAACGTAGGATGAAACCAGCTCAATTATGGACAATCCTCCAAGACGGGACGATTCGAACGATGAATAATAGATATTGTTTGGTTTCTTACCTCCCTCTTGGTATGACCACAACCAATGTGATAGTAAGCTTATGTGAAAAGGAATCAAATCCTAACAAAATATGGAACCACAAGAAAGACGGGACCATAGTCCACGTCGAGTCCGGGATGGTTCTAACAGGAAAATTCGATAGCTATGTGACATTAGAAAGAAACGAAAATGTACCATCACAAAGCTGGGAAGTCACAGAAAATTTAACCCCAATGGTGGCGAACATCAAATGGCTTGACAACTTGTGTTTGCAATCTACGGAAGATTCGAATAATGTGGGGTTGGATGGATGTAATAGAGAAAATAAGAATCAAAGATGGGCATTATATGGAGATGGAACTATTCGACAAAATGTCAATAGAAACTATTGCTTGACTTCTAAAGAAGATTATGGTCATTATATAGCTGTGTCTAAATGTGAAGACATACCACAACAACGTTGGGGGCTTAGTGCAGAAGACAACACTATTAACCATCCAGCCAACATTGATATGGTCTTGGATGTGCTTGATGTGCCATTAAAGTATCCACTAGCGATCTTGATGAGCCATCGTGATGGAAGTCCTAGCCAGCGATGGATTATTTACTGA

Coding sequence (CDS)

ATGAGAGAAATTGTGTTGAGCATTGTTGTGGCTTTCTCACTCACCACCCACCTTGTCATAGCATCGTCCTCAAATTATGGATATGGAGGATTCCCTGGAAGCACACATCTTGTAGGTCGAGATGGATTATGTTTAGAGGCATCTCCATGGTTTACCAAAGGTAATTATTTTTCAACTCGATTATCACCATGCGACGACGAACGTAGGATGAAACCAGCTCAATTATGGACAATCCTCCAAGACGGGACGATTCGAACGATGAATAATAGATATTGTTTGGTTTCTTACCTCCCTCTTGGTATGACCACAACCAATGTGATAGTAAGCTTATGTGAAAAGGAATCAAATCCTAACAAAATATGGAACCACAAGAAAGACGGGACCATAGTCCACGTCGAGTCCGGGATGGTTCTAACAGGAAAATTCGATAGCTATGTGACATTAGAAAGAAACGAAAATGTACCATCACAAAGCTGGGAAGTCACAGAAAATTTAACCCCAATGGTGGCGAACATCAAATGGCTTGACAACTTGTGTTTGCAATCTACGGAAGATTCGAATAATGTGGGGTTGGATGGATGTAATAGAGAAAATAAGAATCAAAGATGGGCATTATATGGAGATGGAACTATTCGACAAAATGTCAATAGAAACTATTGCTTGACTTCTAAAGAAGATTATGGTCATTATATAGCTGTGTCTAAATGTGAAGACATACCACAACAACGTTGGGGGCTTAGTGCAGAAGACAACACTATTAACCATCCAGCCAACATTGATATGGTCTTGGATGTGCTTGATGTGCCATTAAAGTATCCACTAGCGATCTTGATGAGCCATCGTGATGGAAGTCCTAGCCAGCGATGGATTATTTACTGA

Protein sequence

MREIVLSIVVAFSLTTHLVIASSSNYGYGGFPGSTHLVGRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVPLKYPLAILMSHRDGSPSQRWIIY

Homology

[Close]

BLAST of Lsi05G001820 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 2.7e-30
Identity = 83/261 (31.80%), Postives = 137/261 (52.49%), Query Frame = 0


Query: 36  HLVGRDGLCLEA-SPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLV 95
           ++VGRDGLC++  + + T G     +L PC  +R     Q WT   D TIR+M    C+ 
Sbjct: 311 NIVGRDGLCVDVRNGYDTDGT--PLQLWPCGTQRN----QRWTFDSDDTIRSMGK--CMT 370

Query: 96  SYLPLGMTT-TNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLT---GKFDSYVTLER 155
           +    G+   +N+++  C   +     W    DG+I++  SG+V+T       + + LE 
Sbjct: 371 AN---GLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLED 430

Query: 156 NENVPSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGT 215
           N    SQ W VT N+ P+VA+I     +CLQS  ++N V ++ C   +  Q+WALYGD T
Sbjct: 431 NIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRT 490

Query: 216 IRQNVNRNYCLTSK-EDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP 275
           IR N  R  C+T+   +    I + KC+ +P QRW  +++   +N  +   M +   +V 
Sbjct: 491 IRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVS 550

Query: 276 LKYPLAILMSHRDGSPSQRWI 291
           L+    I++    G+P+Q+W+
Sbjct: 551 LR---EIIIFPATGNPNQQWV 557

[Close]

BLAST of Lsi05G001820 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.6e-29
Identity = 84/259 (32.43%), Postives = 134/259 (51.74%), Query Frame = 0


Query: 39  GRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLP 98
           GRDG C E      K      +LS C ++      Q WT   DGTI+++    CL     
Sbjct: 323 GRDGFCAEVKNGDEKDG-TPVQLSSCGEQSN----QQWTFSTDGTIQSLGK--CLT---- 382

Query: 99  LGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLT---GKFDSYVTLERNENVP 158
              T+++V++  C+     +  W    DGTI +  SG+VLT       + V+LE+N +  
Sbjct: 383 ---TSSSVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAA 442

Query: 159 SQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENK---NQRWALYGDGTIR 218
            Q W +  N+ P+V  I   + +CL++   +N+V L  C+ ++    +Q+WALYGDGTIR
Sbjct: 443 RQGW-IVGNVEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIR 502

Query: 219 QNVNRNYCLTSK-EDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVPLK 278
            N +R+ C+TS+ +     I + KC     QRW  + +    N  + + M +D  DVPL+
Sbjct: 503 VNNDRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPLR 562

Query: 279 YPLAILMSHRDGSPSQRWI 291
               I++SH  G+ +Q+WI
Sbjct: 563 ---KIILSHPSGTSNQQWI 563

[Close]

BLAST of Lsi05G001820 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.8e-27
Identity = 89/259 (34.36%), Postives = 133/259 (51.35%), Query Frame = 0


Query: 39  GRDGLCLEA-SPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYL 98
           G DGLC++     +  GN  + +L PC +E      QLWT   DGTIR +    CL    
Sbjct: 327 GWDGLCVDVRDGHYIDGN--TVQLGPCGNECN----QLWTFRTDGTIRWLGK--CLT--- 386

Query: 99  PLGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLT---GKFDSYVTLERNENV 158
               T+++V++  C         W    DGTI +  SG+VLT       + ++LE N + 
Sbjct: 387 ----TSSSVMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSLENNIHA 446

Query: 159 PSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQN 218
             Q W V + + P+V  I     +CL    ++N V L+ C      Q WALYGDGTIR N
Sbjct: 447 ARQGWTVGD-VEPLVTFIVGYKQMCLTENGENNFVWLEDCVLNRVEQEWALYGDGTIRVN 506

Query: 219 VNRNYCLTSKE-DYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVL--DVPLK 278
            NR+ C+TS++ +    I + KCE    QRW  +  + TI++P N  +V+DV   +V L+
Sbjct: 507 SNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNT-NGTISNP-NAKLVMDVAQSNVSLR 564

Query: 279 YPLAILMSHRDGSPSQRWI 291
               I++    G+P+Q+WI
Sbjct: 567 ---KIILYPPTGNPNQQWI 564

[Close]

BLAST of Lsi05G001820 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.1e-27
Identity = 88/259 (33.98%), Postives = 133/259 (51.35%), Query Frame = 0


Query: 39  GRDGLCLEAS-PWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYL 98
           G DGLC++     +  GN    +L PC +E      QLWT   DGTIR +    CL +  
Sbjct: 327 GWDGLCVDVRYGHYIDGN--PVQLRPCGNECN----QLWTFRTDGTIRWLGK--CLTA-- 386

Query: 99  PLGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLT---GKFDSYVTLERNENV 158
                +++V++  C         W    DGTI +  SG+VLT       + ++LE N + 
Sbjct: 387 -----SSSVMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHA 446

Query: 159 PSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQN 218
             Q W V + + P+V  I     +CL+   ++N V L+ C      Q WALYGDGTIR N
Sbjct: 447 ARQGWTVGD-VEPLVTFIVGYKQMCLRENGENNFVWLEDCVLNRVQQEWALYGDGTIRVN 506

Query: 219 VNRNYCLTSKE-DYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVL--DVPLK 278
            NR+ C+TS++ +    I + KCE    QRW  +  + TI++P N  +++DV   DV L+
Sbjct: 507 SNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNT-NGTISNP-NAKLLMDVAQRDVSLR 564

Query: 279 YPLAILMSHRDGSPSQRWI 291
               I++    G+P+Q+WI
Sbjct: 567 ---KIILYRPTGNPNQQWI 564

[Close]

BLAST of Lsi05G001820 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 2.0e-25
Identity = 81/268 (30.22%), Postives = 130/268 (48.51%), Query Frame = 0


Query: 34  STHLVGRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCL 93
           +T + GRD LC++ +   T        L PC  +   K    WT   DGT+R++    CL
Sbjct: 264 TTRISGRDALCVDVAGALTSDG-SRLILYPCGQQVNQK----WTFHSDGTVRSLGK--CL 323

Query: 94  VSYLPLGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVE-SGMVLTGK---FDSYVTLE 153
            +          V++  C K +  +  W+    GTI++     + LT       + +T+E
Sbjct: 324 AT--NNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTME 383

Query: 154 RNENVPSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDG 213
            N    SQ W V   + P++ +I  LD++CL++T+ + N+ L+ C    + Q WALY DG
Sbjct: 384 VNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECVPNQREQSWALYSDG 443

Query: 214 TIRQNVNRNYCLT---SKEDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVL 273
           TIR + NR  C+T   S  D    I +  C+    QRW   A D +I+ P N  + +DV 
Sbjct: 444 TIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLA-DGSISTPGNQRLAMDVA 503

Query: 274 --DVPLKYPLAILMSHRDGSPSQRWIIY 293
             DV LK    I++    G  +Q+W+++
Sbjct: 504 RSDVDLK---KIILHRPHGDLNQQWVLF 518

[Close]

BLAST of Lsi05G001820 vs. ExPASy TrEMBL
Match: A0A0A0L756 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G219180 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.3e-114
Identity = 204/293 (69.62%), Postives = 243/293 (82.94%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYGGFPGSTHLVGRDGLCLEASPWFTKGNYFSTR 60
           M+EIVLSIVVAFSLTTHL +A  S+YGY   PG+THLVGRDGLCLE SPWF KG+ F TR
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 61  LSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESNPNKI 120
           LSPC++++  K  QLWT+LQD TIR MN+RYCLVSY+ +    TNV+VS C KE + NK 
Sbjct: 61  LSPCNEQK--KQTQLWTVLQDETIRPMNDRYCLVSYVSVNF-ITNVVVSECAKEPHSNKK 120

Query: 121 WNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLDNLCL 180
           W HK+D TIVHV SGMVLTG   +YVT++RN+N PSQSWE T++LTPMVANIKWL+NLCL
Sbjct: 121 WIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCL 180

Query: 181 QSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKCEDIP 240
           QST+DSN V LDGCN ENK+Q W+LYGDGTIR++VNRNYCLTS++D+G ++AVSKCED P
Sbjct: 181 QSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDKP 240

Query: 241 QQRWGLSAEDNTINHPANIDMVLDVLDVPL-KYPLAILMSHRDGSPSQRWIIY 293
           QQRWGL AEDNTINHP N DMV+DVL VP    PLA++ +HRDG+ +QRWIIY
Sbjct: 241 QQRWGLGAEDNTINHP-NTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

[Close]

BLAST of Lsi05G001820 vs. ExPASy TrEMBL
Match: A0A5A7T854 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.9e-109
Identity = 199/296 (67.23%), Postives = 236/296 (79.73%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYG----YGGFPGSTHLVGRDGLCLEASPWFTKGNY 60
           M+EIVLSI+VAFSLTTHL +A  SNYG    YGG PG+THLVGRDGLCLE SPWF +GN 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESN 120
           F TRLSPCD+ +  K  QLWT++ DGTIR MN+++CLV+ + +G+     +V  C KESN
Sbjct: 61  FPTRLSPCDEHK--KQTQLWTVVGDGTIRPMNDKFCLVADVLVGV-INKAVVRECAKESN 120

Query: 121 PNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLD 180
            NK W HKKDGTI HVESGMVLTG  D YVTL+ N+N PSQSWE TE+ TPM+ANI+WL+
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLN 180

Query: 181 NLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKC 240
           NLCLQST+DSN+VGL+GCNRENK+QRWALYGDGTIRQNVNRNYCLTS E  G +I +SKC
Sbjct: 181 NLCLQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKC 240

Query: 241 EDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           ED PQQRW L A+D+TI+HP N DMV+DVL VP   +  A++ + RDGS SQRW I
Sbjct: 241 EDKPQQRWNLGAKDSTIDHP-NTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. ExPASy TrEMBL
Match: A0A5D3BL95 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00260 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.9e-108
Identity = 198/296 (66.89%), Postives = 235/296 (79.39%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYG----YGGFPGSTHLVGRDGLCLEASPWFTKGNY 60
           M+EIVLSI+VAFSLTTHL +A   NYG    YGG PG+THLVGRDGLCLE SPWF +GN 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPYNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESN 120
           F TRLSPCD+ +  K  QLWT++ DGTIR MN+++CLV+ + +G+     +V  C KESN
Sbjct: 61  FPTRLSPCDEHK--KQTQLWTVVGDGTIRPMNDKFCLVADVLVGV-INKAVVRECAKESN 120

Query: 121 PNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLD 180
            NK W HKKDGTI HVESGMVLTG  D YVTL+ N+N PSQSWE TE+ TPM+ANI+WL+
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLN 180

Query: 181 NLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKC 240
           NLCLQST+DSN+VGL+GCNRENK+QRWALYGDGTIRQNVNRNYCLTS E  G +I +SKC
Sbjct: 181 NLCLQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKC 240

Query: 241 EDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           ED PQQRW L A+D+TI+HP N DMV+DVL VP   +  A++ + RDGS SQRW I
Sbjct: 241 EDKPQQRWNLGAKDSTIDHP-NTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. ExPASy TrEMBL
Match: A0A1S4E0X9 (nigrin b-like OS=Cucumis melo OX=3656 GN=LOC103496121 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.3e-107
Identity = 197/296 (66.55%), Postives = 234/296 (79.05%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYG----YGGFPGSTHLVGRDGLCLEASPWFTKGNY 60
           M+EIVLSI+VAFSLTTHL +A  SNYG    YGG P +THLVGRDGLCLE SPWF +GN 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESN 120
           F TRLSPCD+ +  K  QLWT++ DGTIR MN+++CLV+ + +G+     +V  C KE N
Sbjct: 61  FPTRLSPCDEHK--KQTQLWTVVGDGTIRPMNDKFCLVADVLVGL-INKAVVRECAKEPN 120

Query: 121 PNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLD 180
            NK W HKKDGTI HVESGMVLTG  D YVTL+ N+N PSQSWE TE+ TPM+ANI+WL+
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLN 180

Query: 181 NLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKC 240
           NLCLQST+DSN+VGL+GCNRENK+QRWALYGDGTIRQNVNRNYCLTS E  G +I +SKC
Sbjct: 181 NLCLQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKC 240

Query: 241 EDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           ED PQQRW L A+D+TI+HP N DMV+DVL VP   +  A++ + RDGS SQRW I
Sbjct: 241 EDKPQQRWNLGAKDSTIDHP-NSDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. ExPASy TrEMBL
Match: A0A0A0L9D1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G217160 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 2.0e-97
Identity = 186/297 (62.63%), Postives = 224/297 (75.42%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNY----GYGGFPGSTHLVGRDGLCLEASPWF-TKGN 60
           M+EIVLSI+VAFSLTT LVIA   NY    GYGG PG+THLVGRDGLCLE SPW+   G 
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGI 60

Query: 61  YFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKES 120
            F TRLSPCD++   K  QLWTI+ DGTIR MN+++CL + +  G+     +VS C K S
Sbjct: 61  NFPTRLSPCDEK---KQTQLWTIVGDGTIRPMNDKFCLAAEVFYGV-INKAVVSECGKVS 120

Query: 121 NPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWL 180
           +PNK W  K DGTI  V+S MVLTG  D YVTL+ N+  PSQSWEVTE+L  MVANI+WL
Sbjct: 121 DPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEWL 180

Query: 181 DNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSK 240
           +NLCLQST+DS++VGL+GCN +NK QRWALY DGTIRQ+VN+NYCLTS +D+G ++ VSK
Sbjct: 181 NNLCLQSTDDSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSK 240

Query: 241 CEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           CED PQQRW L A+D TI+HP N DMVLDV  VP   +P  ++ + RDGS SQRW I
Sbjct: 241 CEDKPQQRWSLDAKDYTIDHP-NTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: XP_038904853.1 (nigrin b-like [Benincasa hispida])

HSP 1 Score: 474.6 bits (1220), Expect = 6.3e-130
Identity = 224/292 (76.71%), Postives = 252/292 (86.30%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYGGFPGSTHLVGRDGLCLEASPWFTKGNYFSTR 60
           M+EIVLSIVVAFSLTTHLV+A   NYGYGGFPGSTHLVGRDGLCLE  PW+ K +YF TR
Sbjct: 1   MKEIVLSIVVAFSLTTHLVMAVRPNYGYGGFPGSTHLVGRDGLCLEMFPWYEKDSYFPTR 60

Query: 61  LSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESNPNKI 120
           LSPC+ ++  K  QLWT+LQDGTIR MN++YCLV+Y  +G+   NV+V+ C KESNPNKI
Sbjct: 61  LSPCNVQK--KQTQLWTVLQDGTIRPMNDKYCLVAYYRVGV-LNNVVVNDCSKESNPNKI 120

Query: 121 WNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLDNLCL 180
           WNHKKDGT+VHVESGMVLTGK D+YVTLE NENVPSQSWE TE+LTPMVANIKWLDNLCL
Sbjct: 121 WNHKKDGTVVHVESGMVLTGKSDAYVTLEPNENVPSQSWEATESLTPMVANIKWLDNLCL 180

Query: 181 QSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKCEDIP 240
           QSTEDSN+VGLDGCNREN  QRWALYGDGTIR +VNRNYCLTS++D G +I VS+CED P
Sbjct: 181 QSTEDSNHVGLDGCNRENMKQRWALYGDGTIRHSVNRNYCLTSEQDVGRFIVVSRCEDKP 240

Query: 241 QQRWGLSAEDNTINHPANIDMVLDVLDVPLKYPLAILMSHRDGSPSQRWIIY 293
           QQRWGLSAED+TINHPAN DMV+DV  VP     A++M HRDGSPSQRWIIY
Sbjct: 241 QQRWGLSAEDSTINHPANTDMVMDVFMVPTTLVPAVVMGHRDGSPSQRWIIY 289

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: XP_004146289.1 (nigrin b [Cucumis sativus] >KGN57583.1 hypothetical protein Csa_010930 [Cucumis sativus])

HSP 1 Score: 420.6 bits (1080), Expect = 1.1e-113
Identity = 204/293 (69.62%), Postives = 243/293 (82.94%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYGGFPGSTHLVGRDGLCLEASPWFTKGNYFSTR 60
           M+EIVLSIVVAFSLTTHL +A  S+YGY   PG+THLVGRDGLCLE SPWF KG+ F TR
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 61  LSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESNPNKI 120
           LSPC++++  K  QLWT+LQD TIR MN+RYCLVSY+ +    TNV+VS C KE + NK 
Sbjct: 61  LSPCNEQK--KQTQLWTVLQDETIRPMNDRYCLVSYVSVNF-ITNVVVSECAKEPHSNKK 120

Query: 121 WNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLDNLCL 180
           W HK+D TIVHV SGMVLTG   +YVT++RN+N PSQSWE T++LTPMVANIKWL+NLCL
Sbjct: 121 WIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCL 180

Query: 181 QSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKCEDIP 240
           QST+DSN V LDGCN ENK+Q W+LYGDGTIR++VNRNYCLTS++D+G ++AVSKCED P
Sbjct: 181 QSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDKP 240

Query: 241 QQRWGLSAEDNTINHPANIDMVLDVLDVPL-KYPLAILMSHRDGSPSQRWIIY 293
           QQRWGL AEDNTINHP N DMV+DVL VP    PLA++ +HRDG+ +QRWIIY
Sbjct: 241 QQRWGLGAEDNTINHP-NTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: KAA0039058.1 (nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 404.4 bits (1038), Expect = 8.1e-109
Identity = 199/296 (67.23%), Postives = 236/296 (79.73%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYG----YGGFPGSTHLVGRDGLCLEASPWFTKGNY 60
           M+EIVLSI+VAFSLTTHL +A  SNYG    YGG PG+THLVGRDGLCLE SPWF +GN 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESN 120
           F TRLSPCD+ +  K  QLWT++ DGTIR MN+++CLV+ + +G+     +V  C KESN
Sbjct: 61  FPTRLSPCDEHK--KQTQLWTVVGDGTIRPMNDKFCLVADVLVGV-INKAVVRECAKESN 120

Query: 121 PNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLD 180
            NK W HKKDGTI HVESGMVLTG  D YVTL+ N+N PSQSWE TE+ TPM+ANI+WL+
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLN 180

Query: 181 NLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKC 240
           NLCLQST+DSN+VGL+GCNRENK+QRWALYGDGTIRQNVNRNYCLTS E  G +I +SKC
Sbjct: 181 NLCLQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKC 240

Query: 241 EDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           ED PQQRW L A+D+TI+HP N DMV+DVL VP   +  A++ + RDGS SQRW I
Sbjct: 241 EDKPQQRWNLGAKDSTIDHP-NTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: TYJ99749.1 (nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 402.1 bits (1032), Expect = 4.0e-108
Identity = 198/296 (66.89%), Postives = 235/296 (79.39%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYG----YGGFPGSTHLVGRDGLCLEASPWFTKGNY 60
           M+EIVLSI+VAFSLTTHL +A   NYG    YGG PG+THLVGRDGLCLE SPWF +GN 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPYNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESN 120
           F TRLSPCD+ +  K  QLWT++ DGTIR MN+++CLV+ + +G+     +V  C KESN
Sbjct: 61  FPTRLSPCDEHK--KQTQLWTVVGDGTIRPMNDKFCLVADVLVGV-INKAVVRECAKESN 120

Query: 121 PNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLD 180
            NK W HKKDGTI HVESGMVLTG  D YVTL+ N+N PSQSWE TE+ TPM+ANI+WL+
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLN 180

Query: 181 NLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKC 240
           NLCLQST+DSN+VGL+GCNRENK+QRWALYGDGTIRQNVNRNYCLTS E  G +I +SKC
Sbjct: 181 NLCLQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKC 240

Query: 241 EDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           ED PQQRW L A+D+TI+HP N DMV+DVL VP   +  A++ + RDGS SQRW I
Sbjct: 241 EDKPQQRWNLGAKDSTIDHP-NTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: XP_016901886.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 399.4 bits (1025), Expect = 2.6e-107
Identity = 197/296 (66.55%), Postives = 234/296 (79.05%), Query Frame = 0


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYG----YGGFPGSTHLVGRDGLCLEASPWFTKGNY 60
           M+EIVLSI+VAFSLTTHL +A  SNYG    YGG P +THLVGRDGLCLE SPWF +GN 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESN 120
           F TRLSPCD+ +  K  QLWT++ DGTIR MN+++CLV+ + +G+     +V  C KE N
Sbjct: 61  FPTRLSPCDEHK--KQTQLWTVVGDGTIRPMNDKFCLVADVLVGL-INKAVVRECAKEPN 120

Query: 121 PNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLD 180
            NK W HKKDGTI HVESGMVLTG  D YVTL+ N+N PSQSWE TE+ TPM+ANI+WL+
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLN 180

Query: 181 NLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKC 240
           NLCLQST+DSN+VGL+GCNRENK+QRWALYGDGTIRQNVNRNYCLTS E  G +I +SKC
Sbjct: 181 NLCLQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKC 240

Query: 241 EDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           ED PQQRW L A+D+TI+HP N DMV+DVL VP   +  A++ + RDGS SQRW I
Sbjct: 241 EDKPQQRWNLGAKDSTIDHP-NSDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

The following BLAST results are available for this feature:

BLAST of Lsi05G001820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33183	2.7e-30	31.80	Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2	[more]
P93543	8.6e-29	32.43	Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1	[more]
O22415	2.8e-27	34.36	Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=...	[more]
Q41358	6.1e-27	33.98	Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1	[more]
U3KRF8	2.0e-25	30.22	Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2	[more]

BLAST of Lsi05G001820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L756	5.3e-114	69.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G219180 PE=4 SV=1	[more]
A0A5A7T854	3.9e-109	67.23	Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 ...	[more]
A0A5D3BL95	1.9e-108	66.89	Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00260 ...	[more]
A0A1S4E0X9	1.3e-107	66.55	nigrin b-like OS=Cucumis melo OX=3656 GN=LOC103496121 PE=4 SV=1	[more]
A0A0A0L9D1	2.0e-97	62.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G217160 PE=4 SV=1	[more]

BLAST of Lsi05G001820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904853.1	6.3e-130	76.71	nigrin b-like [Benincasa hispida]	[more]
XP_004146289.1	1.1e-113	69.62	nigrin b [Cucumis sativus] >KGN57583.1 hypothetical protein Csa_010930 [Cucumis ...	[more]
KAA0039058.1	8.1e-109	67.23	nigrin b-like [Cucumis melo var. makuwa]	[more]
TYJ99749.1	4.0e-108	66.89	nigrin b-like [Cucumis melo var. makuwa]	[more]
XP_016901886.1	2.6e-107	66.55	PREDICTED: nigrin b-like [Cucumis melo]	[more]

BLAST of Lsi05G001820 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 166..292 e-value: 2.2E-17 score: 73.8 coord: 31..162 e-value: 4.8E-7 score: 39.4
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 35..159 e-value: 6.7E-15 score: 55.5 coord: 176..289 e-value: 5.3E-19 score: 68.7
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 31..162 score: 20.388
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 165..292 score: 26.1765
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 36..160 e-value: 7.84409E-14 score: 64.8342
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 171..291 e-value: 4.94757E-16 score: 70.9974
None	No IPR available	GENE3D	2.80.10.50		coord: 32..162 e-value: 5.6E-20 score: 73.5
None	No IPR available	GENE3D	2.80.10.50		coord: 167..292 e-value: 6.2E-29 score: 102.2
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 38..162
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 167..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G001820.1	Lsi05G001820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity