Lsi05G000850 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G000850 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 2.0e-146
Identity = 315/670 (47.01%), Postives = 419/670 (62.54%), Query Frame = 0

Query: 108 SFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKE 167
           S+ +  F E V SKG+ S  K ++    FL  WN + V+ C+IA  LDPLF Y+ ++D  
Sbjct: 64  SYKSYSFKEAV-SKGIGSTHKILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIIDDA 123

Query: 168 ENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARR 227
           + C+  D K+++T  VLRS  D  Y+L I+F FR  + AP+    GR         +A+R
Sbjct: 124 KKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIAKR 183

Query: 228 YLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLK 287
           YL S+FI+DILAVLPLPQ+VIL++I   +GS        LKFI+ FQY+PR  R+Y   K
Sbjct: 184 YLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNTKNMLKFIVFFQYIPRFIRIYPLYK 243

Query: 288 KVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRC-HSYPYCHR 347
           +V  +SGIL ++A A A FNLFLYMLASHVFGAFWYLFS+ER+  C +  C  + P C  
Sbjct: 244 EVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPCIS 303

Query: 348 ----INNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCW 407
                +  ++   + +N+ C  + P+  TT FD+GIF DA  SGVV S DF  KF YC W
Sbjct: 304 KLLYCDPETAGGNAFLNESCPIQTPN--TTLFDFGIFLDALQSGVVESQDFPQKFFYCFW 363

Query: 408 WGLQNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIA 467
           WGLQNL                             SSLGQ LKTS +IWEI FAV I+IA
Sbjct: 364 WGLQNL-----------------------------SSLGQNLKTSTYIWEICFAVFISIA 423

Query: 468 GLVLFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRK 527
           GLVLF+ LIGN    LQ+T  RLEEMR+K +D E WM++  LP +LRKRI++YE+YKW++
Sbjct: 424 GLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQE 483

Query: 528 TRGIDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERN 587
           TRG+D  N+L +LP+DLRRD  RHLCL  +  V MF+ MDE+ LDA+C  L+P+LY E +
Sbjct: 484 TRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEES 543

Query: 588 FIVREGEPLEEMIFIIHGK-LWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHL 647
           +IVREG+P++EM+FI+ GK L I +N  R+G    +SE L  GDF GE+LL W L DPH 
Sbjct: 544 YIVREGDPVDEMLFIMRGKLLTITTNGGRTGFL--NSEYLGAGDFCGEELLTWAL-DPHS 603

Query: 648 TT-VPISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRND-PNFKERWAPLAAVV 707
           ++ +PIST+TV    +VEAF L A+DLK V S+F  L S++LR+    + ++W   AA  
Sbjct: 604 SSNLPISTRTVRALMEVEAFALKADDLKFVASQFRRLHSKQLRHTFRYYSQQWKTWAACF 663

Query: 708 LQAAWRRYFKS------KREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQR 754
           +QAAWRRY K       K E  + Q +L  E   S PS    T++ASRF A  L  +   
Sbjct: 664 IQAAWRRYIKKKLEESLKEEENRLQDALAKEACGSSPS-LGATIYASRFAANILRTI--- 693

BLAST of Lsi05G000850 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 464.2 bits (1193), Expect = 3.0e-129
Identity = 276/658 (41.95%), Postives = 386/658 (58.66%), Query Frame = 0

Query: 113 HFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEENCIE 172
           H D + K     + K  I     FL  WN + +  CV+A  +DPLF YI +VD   +C+ 
Sbjct: 52  HKDPDHKETSSVTRKNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLT 111

Query: 173 FDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARRYLLSY 232
            D+KL++   +LR+L+D  YI+ IVF FR  Y AP+    GR         +A +YL SY
Sbjct: 112 LDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSY 171

Query: 233 FIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKKVRWS 292
           FI+D+L++LPLPQ+V+L VI         +    LKF +I QY+PR+ R+Y    +V  +
Sbjct: 172 FIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRT 231

Query: 293 SGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCHRINNNSS 352
           SGI+ ++A A A +NL LYMLASHVFGA WYL SVER+  C Q  C     C+       
Sbjct: 232 SGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCE 291

Query: 353 FERSCINDFCSRK------GPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQ 412
            +R+  N+F +        G    +T F++GIF DA  SGVV S DF  KF YC WWGL+
Sbjct: 292 NDRNVSNNFLTTSCPFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLR 351

Query: 413 NLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVL 472
           NL                             S+LGQ L+TSK + EI FA+SI I+GLVL
Sbjct: 352 NL-----------------------------SALGQNLQTSKFVGEIIFAISICISGLVL 411

Query: 473 FALLIGNLQ----ATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGI 532
           FALLIGN+Q    +T  R EEMR++ +D E WM++  LP DLRKRI++YE+Y+W++TRG+
Sbjct: 412 FALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGV 471

Query: 533 DVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVR 592
           +   +L +LP+DLRRD  RHLCL  +K V +F+ MDE+ LDAVC  L+P+LY E ++++R
Sbjct: 472 EEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAVCDRLRPVLYTENSYVIR 531

Query: 593 EGEPLEEMIFIIHGKLWIYSNTSRSGETS-GSSESLTKGDFFGEDLLKWVLKDPHLTTVP 652
           EG+P+ EM+F++ G+L   S T+  G +   ++ +L   DF GEDLL W L     +  P
Sbjct: 532 EGDPVGEMLFVMRGRL--VSATTNGGRSGFFNAVNLKASDFCGEDLLPWALDPQSSSHFP 591

Query: 653 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNF-KERWAPLAAVVLQAAW 712
           IST+TV   T+VEAF L+A DLK+V S+F  L S++L++   F   +W   +   +QAAW
Sbjct: 592 ISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFYSVQWRTWSVSFIQAAW 651

Query: 713 RRY-----FKSKREREKSQPSLVIENGNSQPSATTTT-------LHASRFIARALHAL 741
           RRY      KS R+ E      +        +AT ++       L+ASRF + ALH L
Sbjct: 652 RRYCRRKLAKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGALYASRFASNALHNL 678

BLAST of Lsi05G000850 vs. ExPASy Swiss-Prot
Match: Q9LD40 (Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 GN=CNGC13 PE=3 SV=2)

HSP 1 Score: 453.8 bits (1166), Expect = 4.0e-126
Identity = 281/687 (40.90%), Postives = 394/687 (57.35%), Query Frame = 0

Query: 83  HGDIRSTNSMGSVPTRLESSADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWND 142
           +G  ++  S+ +V   +        SFG+ H  +   +   ++ K  I     FL  WN 
Sbjct: 23  YGRNKARPSLNTVLKNVRRGLKKPLSFGS-HNKKRDSNSSTTTQKNIINPQGSFLQNWNK 82

Query: 143 VLVMLCVIATLLDPLFCYILVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRF 202
           + +   VIA  +DPLF YI +VD E +C+     L++   VLR+ +D  YI+ IVF FR 
Sbjct: 83  IFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFYIIHIVFQFRT 142

Query: 203 RYTAPNDANSGR------LCTLARRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTI 262
            Y +P+    GR         +A +YL SYFI+D+L++LPLPQ+V+L VI         I
Sbjct: 143 AYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVIPNVNKPVSLI 202

Query: 263 AIRSLKFILIFQYLPRVSRVYSFLKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFW 322
               L  ++  QY+PR+ R+Y    +V  +SGI+ ++A A A +NL LYMLASHVFGA W
Sbjct: 203 TKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASHVFGALW 262

Query: 323 YLFSVERKASCLQVRCHSYP--------YCHRINNNSSFERSCINDFCSRKGPSN--GTT 382
           YL SVER+  C +  C   P        YC   + NSS     +   C    P +   +T
Sbjct: 263 YLISVEREDRCWREACEKIPEVCNFRFLYC---DGNSSVRNDFLTTSCPFINPDDITNST 322

Query: 383 AFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQNLRSVFKSLCIMYYYSTPTLRLHISP 442
            F++GIF DA  SG+V S DF  KF YC WWGL+NL                        
Sbjct: 323 VFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNL------------------------ 382

Query: 443 LPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVLFALLIGNLQ----ATIARLEEMRLKG 502
                S+LGQ L TSK + EI FAVSI I+GLVLFALLIGN+Q    +T  R EEMR++ 
Sbjct: 383 -----SALGQNLNTSKFVGEIIFAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRK 442

Query: 503 QDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGIDVANILHSLPRDLRRDTTRHLCLRAI 562
           +D E WM++  LP DLRKRI++YE+YKW++TRG++  N+L +LP+DLRRD  RH CL  +
Sbjct: 443 RDAEQWMSHRMLPDDLRKRIRRYEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLL 502

Query: 563 KSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVREGEPLEEMIFIIHGKLWIYSNTSRSG 622
           K V +F+ MDE+ LDAVC  LKP+LY E ++ +REG+P+EEM+F++ GKL   S T+  G
Sbjct: 503 KKVPLFEIMDEQLLDAVCDKLKPVLYTENSYAIREGDPVEEMLFVMRGKL--MSATTNGG 562

Query: 623 ETS-GSSESLTKGDFFGEDLLKWVLKDPHLTTVPISTKTVSTHTKVEAFVLSANDLKNVV 682
            T   ++  L   DF GEDLL W L     +  PIST+TV   T+VEAF L+A+DLK V 
Sbjct: 563 RTGFFNAVYLKPSDFCGEDLLTWALDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVA 622

Query: 683 SKFWWLISRELRNDPNF-KERWAPLAAVVLQAAWRRYFKSK------REREKSQPSLVIE 741
           S+F  L S++L++   F   +W    A  +QAAWRR+ + K       E ++ + ++   
Sbjct: 623 SQFRRLHSKQLQHTFRFYSVQWRTWGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKR 674

BLAST of Lsi05G000850 vs. ExPASy Swiss-Prot
Match: Q9SKD7 (Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 GN=CNGC3 PE=2 SV=2)

HSP 1 Score: 444.1 bits (1141), Expect = 3.2e-123
Identity = 285/734 (38.83%), Postives = 414/734 (56.40%), Query Frame = 0

Query: 52  QQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNSMGSVPTRLESSADTLTSFG- 111
           Q+   +  N  DD      FS +     +SS         M +V    E  ++ + +F  
Sbjct: 5   QRNKFVRFNGNDD-----EFSTKTTRPSVSS--------VMKTVRRSFEKGSEKIRTFKR 64

Query: 112 --NIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEE 171
             ++H ++  ++     I + +  N  +L  WN + ++L V+A   DPLF YI  V  E 
Sbjct: 65  PLSVHSNKNKENNKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPER 124

Query: 172 NCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARRY 231
            C+  D KL+    V R+ +D  Y++ ++F F   +  P+ +  GR         +A RY
Sbjct: 125 FCLNLDKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRY 184

Query: 232 LLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKK 291
           L SYF++D+L++LP+PQVV+L ++   +     +A   LK+++  QY+PR++R+Y   K+
Sbjct: 185 LGSYFLIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKE 244

Query: 292 VRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRC-------HSY 351
           V  +SG++ ++A A A  NLFLYMLASHVFG+FWYL S+ERK  C +  C       H+Y
Sbjct: 245 VTRTSGLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAY 304

Query: 352 PYCHRINNNSSFERSCINDFCSRKGPS--NGTTAFDYGIFNDAFNSGVVFSTDFIWKFSY 411
            YC     ++   R  +N  C    P     +T F++GIF DA  SGVV S DF  KF Y
Sbjct: 305 LYCSPTGEDN---RLFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFY 364

Query: 412 CCWWGLQNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSI 471
           C WWGL+NL                             S+LGQ LKTS    EI FA+ I
Sbjct: 365 CFWWGLRNL-----------------------------SALGQNLKTSAFEGEIIFAIVI 424

Query: 472 TIAGLVLFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYK 531
            I+GLVLFALLIGN    LQ+T  R+EEMR+K +D E WM++  LP DLRKRI++YE+YK
Sbjct: 425 CISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAEQWMSHRMLPDDLRKRIRKYEQYK 484

Query: 532 WRKTRGIDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYI 591
           W++T+G++   +L SLP+DLR+D  RHLCL+ +K V  FQ MD++ LDA+C  LK +LY 
Sbjct: 485 WQETKGVEEEALLSSLPKDLRKDIKRHLCLKLLKKVPWFQAMDDRLLDALCARLKTVLYT 544

Query: 592 ERNFIVREGEPLEEMIFIIHGKLWIYSNTSRSGETS-GSSESLTKGDFFGEDLLKWVLKD 651
           E+++IVREGEP+E+M+FI+ G L   S T+  G T   +S  L  GDF G DLL W L D
Sbjct: 545 EKSYIVREGEPVEDMLFIMRGNL--ISTTTYGGRTGFFNSVDLVAGDFCG-DLLTWAL-D 604

Query: 652 PHLTTVPISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNF-KERWAPLAA 711
           P  +  PIS++TV   T+VE F+LSA+DLK V +++  L S++LR+   F   +W   AA
Sbjct: 605 PLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQYRRLHSKQLRHMFRFYSVQWQTWAA 664

Query: 712 VVLQAAWRRYFKSK-----RERE-KSQPSLVIENGNSQPSATTTTLHASRFIARALHALN 756
             +QAAW+R+ + K     RE E K   +L  ++           ++ASRF   A HAL 
Sbjct: 665 CFIQAAWKRHCRRKLSKALREEEGKLHNTLQNDDSGGNKLNLGAAIYASRF---ASHALR 686

BLAST of Lsi05G000850 vs. ExPASy Swiss-Prot
Match: Q9FXH6 (Putative cyclic nucleotide-gated ion channel 8 OS=Arabidopsis thaliana OX=3702 GN=CNGC8 PE=3 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 1.0e-121
Identity = 266/658 (40.43%), Postives = 380/658 (57.75%), Query Frame = 0

Query: 125 SIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEENCIEFDNKLKVTVVVL 184
           S KK  +     L LWN + V+ C++A  +DPLF Y+ +VD  +NCI  D+KL VT   L
Sbjct: 93  SEKKIFDPQDKTLLLWNRMFVISCILAVSVDPLFFYLPIVDNSKNCIGIDSKLAVTTTTL 152

Query: 185 RSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARRYLLSYFIVDILAVLPLP 244
           R+++D  Y+  +   FR  Y AP+    GR         +A RYL  YFIVD LAVLPLP
Sbjct: 153 RTIIDVFYLTRMALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFIVDFLAVLPLP 212

Query: 245 QVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKKVRWSSGILPDSAGAKA 304
           Q+ +   + G+KG+ +    ++L  I+I QY+PR  R      +++ ++G   + A A A
Sbjct: 213 QIAVWKFLHGSKGTDVLPTKQALLHIVITQYIPRFVRFIPLTSELKKTAGAFAEGAWAGA 272

Query: 305 IFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYP---------YCH---RINNNSS 364
            + L  YMLASH+ GAFWY+ SVER  +CL+  C   P         YC      +  + 
Sbjct: 273 AYYLLWYMLASHITGAFWYMLSVERNDTCLRSACKVQPDPKVCVQILYCGSKLMSSRETD 332

Query: 365 FERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQNLRSVF 424
           + +S  + F +     +  + F+YGI++ A +SG+V ST F  KF YC WWGLQNL    
Sbjct: 333 WIKSVPDLFKNNCSAKSDESKFNYGIYSQAVSSGIVSSTTFFSKFCYCLWWGLQNL---- 392

Query: 425 KSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVLFALLIG 484
                                    S+LGQGL+TS +  E+ F+++I +AGL+LFALLIG
Sbjct: 393 -------------------------STLGQGLQTSTYPGEVLFSIAIAVAGLLLFALLIG 452

Query: 485 N----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGIDVANIL 544
           N    LQ+   RLEEMR+K +D E WM + SLP++LR+R+++Y++YKW +TRG+D  NI+
Sbjct: 453 NMQTYLQSLTVRLEEMRIKRRDSEQWMHHRSLPQNLRERVRRYDQYKWLETRGVDEENIV 512

Query: 545 HSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVREGEPLE 604
            SLP+DLRRD  RHLCL  ++ V +F NMDE+ LDA+C  LKP LY E  +IVREG+P+ 
Sbjct: 513 QSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTESTYIVREGDPVN 572

Query: 605 EMIFIIHGKLWIYSNTSRSGETSGSSESLTK-GDFFGEDLLKWVLKDPHLTTVPISTKTV 664
           EM+FII G+L   S T+  G +   +  L K GDF GE+LL W L     + +P ST+TV
Sbjct: 573 EMLFIIRGRL--ESVTTDGGRSGFFNRGLLKEGDFCGEELLTWALDPKAGSNLPSSTRTV 632

Query: 665 STHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNF-KERWAPLAAVVLQAAWRRYFKS 724
              T+VEAF L A +LK V S+F  L SR+++    F  ++W   AA  +QAAWRR+ + 
Sbjct: 633 KALTEVEAFALEAEELKFVASQFRRLHSRQVQQTFRFYSQQWRTWAACFIQAAWRRHLRR 692

Query: 725 K----REREKSQP---------------SLVIENGNSQPSATT--TTLHASRFIARAL 738
           K    R +E+ +                 +V  + +S  S++T  +T+ ASRF A AL
Sbjct: 693 KIAELRRKEEEEEEMDYEDDEYYDDNMGGMVTRSDSSVGSSSTLRSTVFASRFAANAL 719

BLAST of Lsi05G000850 vs. ExPASy TrEMBL
Match: A0A0A0L8K4 (Cyclic nucleotide-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G607120 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 588/713 (82.47%), Postives = 628/713 (88.08%), Query Frame = 0

Query: 59  MNAEDDAIRIQSFSREHQAEEISSHGDIRSTNSMGSVPTRLESSADTLTSFGNIHFDEEV 118
           MN EDDAIRI+SFS EHQ+E +SSH DI STNS+GSVPTRLES  D LTSFGNIHFDEEV
Sbjct: 1   MNVEDDAIRIRSFSHEHQSENLSSHVDIHSTNSVGSVPTRLESPTDKLTSFGNIHFDEEV 60

Query: 119 KSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEENCIEFDNKLK 178
           KSKGLSSIK+SIEGNLLFLHLWNDVLV++CVIATLLDPLFCYIL+VD+E++CIEFDNKL 
Sbjct: 61  KSKGLSSIKRSIEGNLLFLHLWNDVLVLMCVIATLLDPLFCYILLVDEEKSCIEFDNKLM 120

Query: 179 VTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTLARRYLLSYFIVDILAVLPLP 238
           VTVVVLRSLVDFGYILLIVFHFR  YTAPNDA+SGRLCTLA RYLLSYF VD+LAVLPLP
Sbjct: 121 VTVVVLRSLVDFGYILLIVFHFRIGYTAPNDASSGRLCTLATRYLLSYFTVDVLAVLPLP 180

Query: 239 QVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKKVRWSSGILPDSAGAKA 298
           QVVIL+V QGTK SHLT A+RSLKFILIFQY PRVSRVY FLKKVRWSSGILPDSAG KA
Sbjct: 181 QVVILIVTQGTKASHLT-ALRSLKFILIFQYFPRVSRVYLFLKKVRWSSGILPDSAGVKA 240

Query: 299 IFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCHRINNNSSFERSCINDFCSR 358
           +FNLFLYMLASH FGAFWYLFSVERKASC+Q+RC+S+PYC R+NNNSSFERSCIND CS 
Sbjct: 241 LFNLFLYMLASHAFGAFWYLFSVERKASCVQIRCNSHPYCSRMNNNSSFERSCINDVCSG 300

Query: 359 KGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQNLRSVFKSLCIMYYYSTP 418
              SN TTA DYGIF+DA NSGVV STDFIWKFSYCCWWGLQNL                
Sbjct: 301 TA-SNVTTALDYGIFDDALNSGVVSSTDFIWKFSYCCWWGLQNL---------------- 360

Query: 419 TLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVLFALLIGN----LQATIAR 478
                        SSLGQGLKTSK IWEIYFA+SITIAGLVLFALLIGN    LQATIAR
Sbjct: 361 -------------SSLGQGLKTSKDIWEIYFAISITIAGLVLFALLIGNLQTYLQATIAR 420

Query: 479 LEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGIDVANILHSLPRDLRRDTT 538
           LEEMRLKGQDIELWMAYHSLPRDLR RIKQYEKYKWRKTRG+DVANIL++LP+DLRRDTT
Sbjct: 421 LEEMRLKGQDIELWMAYHSLPRDLRNRIKQYEKYKWRKTRGVDVANILNNLPKDLRRDTT 480

Query: 539 RHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVREGEPLEEMIFIIHGKLWI 598
           RHLCLRAIKSVSMFQN DEKFLDAVC +LKP LYIERNFIVREGEPL+EMIFIIHGKLWI
Sbjct: 481 RHLCLRAIKSVSMFQNTDEKFLDAVCSYLKPRLYIERNFIVREGEPLDEMIFIIHGKLWI 540

Query: 599 YSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVPISTKTVSTHTKVEAFVLSA 658
           YSN+SRS E SGSSESLTKGDFFGEDLLKWVLKDP LTTVP+STKTVSTHTKVEAFVL+A
Sbjct: 541 YSNSSRSDEISGSSESLTKGDFFGEDLLKWVLKDPLLTTVPMSTKTVSTHTKVEAFVLTA 600

Query: 659 NDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWRRYFKSKREREKSQPSLVIE 718
           NDLKNVVSKFWWLISRELRNDPN KERWA  AAVVLQAAWRRYFK+KREREK + S+V+E
Sbjct: 601 NDLKNVVSKFWWLISRELRNDPNLKERWAAWAAVVLQAAWRRYFKNKREREKCEQSVVVE 660

Query: 719 NGNSQPSATTTTLHASRFIARALHALNQRRKRS-GSNDEPGPSNSNDLQEQSN 767
            GN QP+ATTT+LHASRFIAR + ALN RRK+  GSNDE GPSNSN LQEQSN
Sbjct: 661 IGNPQPAATTTSLHASRFIARIVRALNHRRKKGIGSNDEAGPSNSNGLQEQSN 682

BLAST of Lsi05G000850 vs. ExPASy TrEMBL
Match: A0A6J1FZ85 (cyclic nucleotide-gated ion channel 1-like isoform X6 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 3.2e-296
Identity = 551/733 (75.17%), Postives = 600/733 (81.86%), Query Frame = 0

Query: 45  WLLENAVQQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNS---MGSVPTRLES 104
           W   + V  +   AMN ED AIRIQ+          SS  DIRS NS   +G+VP R ES
Sbjct: 14  WKDIHPVSGKKEAAMNGEDAAIRIQT----------SSPVDIRSENSTGTVGAVPRRFES 73

Query: 105 SADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYI 164
           S+DTLTSFGNIHF EEVKSKGLSSIK+SIEGN +FLHLWNDVLVMLCVIA LLDPLFCYI
Sbjct: 74  SSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAALLDPLFCYI 133

Query: 165 LVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTL--A 224
           LVVD E+NCI FD KL++T VVLRSL+DFGYILLIVFHFR  YT   DAN+G L T+  A
Sbjct: 134 LVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNGTLSTIATA 193

Query: 225 RRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSF 284
           RRYLLSY  VDILA LPLPQVVI+L  Q  KGSH TI+IR+LKF+LI QYLPRV RVY F
Sbjct: 194 RRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLPRVFRVYLF 253

Query: 285 LKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCH 344
           LKKVRWSSGILPDSA AKAIFNLFLYMLASHVFGAFWYLFS+ERKA+CLQ RCHS+PYC 
Sbjct: 254 LKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSRCHSHPYCP 313

Query: 345 RINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGL 404
           R   N + E SC+ D CSRK  SN T  F++GIF+DAF   VV STDFIWK SYC WWGL
Sbjct: 314 R-KYNITEEGSCL-DNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVSYCYWWGL 373

Query: 405 QNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLV 464
           QNL                             SSLGQGL+TSKHIWEIYFAVSITIAGLV
Sbjct: 374 QNL-----------------------------SSLGQGLRTSKHIWEIYFAVSITIAGLV 433

Query: 465 LFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRG 524
           LFALLIGN    LQ+TIARLEEMRLKGQDIELWMAYHSLPRDLRKRIK YEKYKWRKTRG
Sbjct: 434 LFALLIGNLQTFLQSTIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRG 493

Query: 525 IDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIV 584
           +DVANILH+LPRDLRRDTTRHLCL AIKSVSMFQNMDEKFLDAVCG LKPMLYIERNFIV
Sbjct: 494 VDVANILHNLPRDLRRDTTRHLCLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIV 553

Query: 585 REGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVP 644
           REGEPL+EMIFIIHGKLWIYSN++R GET GSSESL KGD FGE+LLKWVLKDP L+TVP
Sbjct: 554 REGEPLDEMIFIIHGKLWIYSNSNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVP 613

Query: 645 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWR 704
           ISTKTVSTHTKVEAFVLSA+DLKNVVSKFWWLIS+E  NDPNFKERWAPLAA+V+QAAWR
Sbjct: 614 ISTKTVSTHTKVEAFVLSAHDLKNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWR 673

Query: 705 RYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQRRK-RSGSNDEPG 764
           RYFKSKREREKSQ SL  E+GNSQPS  TTT+HASRFIARAL+AL +R+K R+ S+D PG
Sbjct: 674 RYFKSKREREKSQLSLATESGNSQPS-VTTTIHASRFIARALNALKRRQKRRNASSDMPG 704

Query: 765 PSNSNDLQEQSNV 768
            SN+NDL + SN+
Sbjct: 734 TSNNNDLPKHSNI 704

BLAST of Lsi05G000850 vs. ExPASy TrEMBL
Match: A0A6J1FZ76 (cyclic nucleotide-gated ion channel 1-like isoform X7 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 4.2e-296
Identity = 551/733 (75.17%), Postives = 600/733 (81.86%), Query Frame = 0

Query: 45  WLLENAVQQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNS---MGSVPTRLES 104
           W   + V  +   AMN ED AIRIQ+          SS  DIRS NS   +G+VP R ES
Sbjct: 14  WKDIHPVSGKKEAAMNGEDAAIRIQT----------SSPVDIRSENSTGTVGAVPRRFES 73

Query: 105 SADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYI 164
           S+DTLTSFGNIHF EEVKSKGLSSIK+SIEGN +FLHLWNDVLVMLCVIA LLDPLFCYI
Sbjct: 74  SSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAALLDPLFCYI 133

Query: 165 LVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTL--A 224
           LVVD E+NCI FD KL++T VVLRSL+DFGYILLIVFHFR  YT   DAN+G L T+  A
Sbjct: 134 LVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNGTLSTIATA 193

Query: 225 RRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSF 284
           RRYLLSY  VDILA LPLPQVVI+L  Q  KGSH TI+IR+LKF+LI QYLPRV RVY F
Sbjct: 194 RRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLPRVFRVYLF 253

Query: 285 LKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCH 344
           LKKVRWSSGILPDSA AKAIFNLFLYMLASHVFGAFWYLFS+ERKA+CLQ RCHS+PYC 
Sbjct: 254 LKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSRCHSHPYCP 313

Query: 345 RINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGL 404
           R   N + E SC+ D CSRK  SN T  F++GIF+DAF   VV STDFIWK SYC WWGL
Sbjct: 314 R-KYNITEEGSCL-DNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVSYCYWWGL 373

Query: 405 QNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLV 464
           QNL                             SSLGQGL+TSKHIWEIYFAVSITIAGLV
Sbjct: 374 QNL-----------------------------SSLGQGLRTSKHIWEIYFAVSITIAGLV 433

Query: 465 LFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRG 524
           LFALLIGN    LQ+TIARLEEMRLKGQDIELWMAYHSLPRDLRKRIK YEKYKWRKTRG
Sbjct: 434 LFALLIGNLQTFLQSTIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRG 493

Query: 525 IDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIV 584
           +DVANILH+LPRDLRRDTTRHLCL AIKSVSMFQNMDEKFLDAVCG LKPMLYIERNFIV
Sbjct: 494 VDVANILHNLPRDLRRDTTRHLCLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIV 553

Query: 585 REGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVP 644
           REGEPL+EMIFIIHGKLWIYSN++R GET GSSESL KGD FGE+LLKWVLKDP L+TVP
Sbjct: 554 REGEPLDEMIFIIHGKLWIYSNSNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVP 613

Query: 645 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWR 704
           ISTKTVSTHTKVEAFVLSA+DLKNVVSKFWWLIS+E  NDPNFKERWAPLAA+V+QAAWR
Sbjct: 614 ISTKTVSTHTKVEAFVLSAHDLKNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWR 673

Query: 705 RYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQRRK-RSGSNDEPG 764
           RYFKSKREREKSQ SL  E+GNSQPS  TTT+HASRFIARAL+AL +R+K R+ S+D PG
Sbjct: 674 RYFKSKREREKSQLSLATESGNSQPS-VTTTIHASRFIARALNALKRRQKRRNASSDMPG 704

Query: 765 PSNSNDLQEQSNV 768
            SN+NDL + SN+
Sbjct: 734 TSNNNDLLKHSNI 704

BLAST of Lsi05G000850 vs. ExPASy TrEMBL
Match: A0A6J1FZB6 (cyclic nucleotide-gated ion channel 1-like isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 4.7e-295
Identity = 549/727 (75.52%), Postives = 596/727 (81.98%), Query Frame = 0

Query: 45  WLLENAVQQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNS---MGSVPTRLES 104
           W   + V  +   AMN ED AIRIQ+          SS  DIRS NS   +G+VP R ES
Sbjct: 14  WKDIHPVSGKKEAAMNGEDAAIRIQT----------SSPVDIRSENSTGTVGAVPRRFES 73

Query: 105 SADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYI 164
           S+DTLTSFGNIHF EEVKSKGLSSIK+SIEGN +FLHLWNDVLVMLCVIA LLDPLFCYI
Sbjct: 74  SSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAALLDPLFCYI 133

Query: 165 LVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTL--A 224
           LVVD E+NCI FD KL++T VVLRSL+DFGYILLIVFHFR  YT   DAN+G L T+  A
Sbjct: 134 LVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNGTLSTIATA 193

Query: 225 RRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSF 284
           RRYLLSY  VDILA LPLPQVVI+L  Q  KGSH TI+IR+LKF+LI QYLPRV RVY F
Sbjct: 194 RRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLPRVFRVYLF 253

Query: 285 LKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCH 344
           LKKVRWSSGILPDSA AKAIFNLFLYMLASHVFGAFWYLFS+ERKA+CLQ RCHS+PYC 
Sbjct: 254 LKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSRCHSHPYCP 313

Query: 345 RINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGL 404
           R   N + E SC+ D CSRK  SN T  F++GIF+DAF   VV STDFIWK SYC WWGL
Sbjct: 314 R-KYNITEEGSCL-DNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVSYCYWWGL 373

Query: 405 QNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLV 464
           QNL                             SSLGQGL+TSKHIWEIYFAVSITIAGLV
Sbjct: 374 QNL-----------------------------SSLGQGLRTSKHIWEIYFAVSITIAGLV 433

Query: 465 LFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRG 524
           LFALLIGN    LQ+TIARLEEMRLKGQDIELWMAYHSLPRDLRKRIK YEKYKWRKTRG
Sbjct: 434 LFALLIGNLQTFLQSTIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRG 493

Query: 525 IDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIV 584
           +DVANILH+LPRDLRRDTTRHLCL AIKSVSMFQNMDEKFLDAVCG LKPMLYIERNFIV
Sbjct: 494 VDVANILHNLPRDLRRDTTRHLCLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIV 553

Query: 585 REGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVP 644
           REGEPL+EMIFIIHGKLWIYSN++R GET GSSESL KGD FGE+LLKWVLKDP L+TVP
Sbjct: 554 REGEPLDEMIFIIHGKLWIYSNSNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVP 613

Query: 645 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWR 704
           ISTKTVSTHTKVEAFVLSA+DLKNVVSKFWWLIS+E  NDPNFKERWAPLAA+V+QAAWR
Sbjct: 614 ISTKTVSTHTKVEAFVLSAHDLKNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWR 673

Query: 705 RYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQRRK-RSGSNDEPG 762
           RYFKSKREREKSQ SL  E+GNSQPS  TTT+HASRFIARAL+AL +R+K R+ S+D PG
Sbjct: 674 RYFKSKREREKSQLSLATESGNSQPS-VTTTIHASRFIARALNALKRRQKRRNASSDMPG 698

BLAST of Lsi05G000850 vs. ExPASy TrEMBL
Match: A0A6J1FZ71 (cyclic nucleotide-gated ion channel 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 4.7e-295
Identity = 549/727 (75.52%), Postives = 596/727 (81.98%), Query Frame = 0

Query: 45  WLLENAVQQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNS---MGSVPTRLES 104
           W   + V  +   AMN ED AIRIQ+          SS  DIRS NS   +G+VP R ES
Sbjct: 14  WKDIHPVSGKKEAAMNGEDAAIRIQT----------SSPVDIRSENSTGTVGAVPRRFES 73

Query: 105 SADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYI 164
           S+DTLTSFGNIHF EEVKSKGLSSIK+SIEGN +FLHLWNDVLVMLCVIA LLDPLFCYI
Sbjct: 74  SSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAALLDPLFCYI 133

Query: 165 LVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTL--A 224
           LVVD E+NCI FD KL++T VVLRSL+DFGYILLIVFHFR  YT   DAN+G L T+  A
Sbjct: 134 LVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNGTLSTIATA 193

Query: 225 RRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSF 284
           RRYLLSY  VDILA LPLPQVVI+L  Q  KGSH TI+IR+LKF+LI QYLPRV RVY F
Sbjct: 194 RRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLPRVFRVYLF 253

Query: 285 LKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCH 344
           LKKVRWSSGILPDSA AKAIFNLFLYMLASHVFGAFWYLFS+ERKA+CLQ RCHS+PYC 
Sbjct: 254 LKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSRCHSHPYCP 313

Query: 345 RINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGL 404
           R   N + E SC+ D CSRK  SN T  F++GIF+DAF   VV STDFIWK SYC WWGL
Sbjct: 314 R-KYNITEEGSCL-DNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVSYCYWWGL 373

Query: 405 QNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLV 464
           QNL                             SSLGQGL+TSKHIWEIYFAVSITIAGLV
Sbjct: 374 QNL-----------------------------SSLGQGLRTSKHIWEIYFAVSITIAGLV 433

Query: 465 LFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRG 524
           LFALLIGN    LQ+TIARLEEMRLKGQDIELWMAYHSLPRDLRKRIK YEKYKWRKTRG
Sbjct: 434 LFALLIGNLQTFLQSTIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRG 493

Query: 525 IDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIV 584
           +DVANILH+LPRDLRRDTTRHLCL AIKSVSMFQNMDEKFLDAVCG LKPMLYIERNFIV
Sbjct: 494 VDVANILHNLPRDLRRDTTRHLCLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIV 553

Query: 585 REGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVP 644
           REGEPL+EMIFIIHGKLWIYSN++R GET GSSESL KGD FGE+LLKWVLKDP L+TVP
Sbjct: 554 REGEPLDEMIFIIHGKLWIYSNSNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVP 613

Query: 645 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWR 704
           ISTKTVSTHTKVEAFVLSA+DLKNVVSKFWWLIS+E  NDPNFKERWAPLAA+V+QAAWR
Sbjct: 614 ISTKTVSTHTKVEAFVLSAHDLKNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWR 673

Query: 705 RYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQRRK-RSGSNDEPG 762
           RYFKSKREREKSQ SL  E+GNSQPS  TTT+HASRFIARAL+AL +R+K R+ S+D PG
Sbjct: 674 RYFKSKREREKSQLSLATESGNSQPS-VTTTIHASRFIARALNALKRRQKRRNASSDMPG 698

BLAST of Lsi05G000850 vs. NCBI nr
Match: XP_004150318.1 (cyclic nucleotide-gated ion channel 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 588/713 (82.47%), Postives = 628/713 (88.08%), Query Frame = 0

Query: 59  MNAEDDAIRIQSFSREHQAEEISSHGDIRSTNSMGSVPTRLESSADTLTSFGNIHFDEEV 118
           MN EDDAIRI+SFS EHQ+E +SSH DI STNS+GSVPTRLES  D LTSFGNIHFDEEV
Sbjct: 1   MNVEDDAIRIRSFSHEHQSENLSSHVDIHSTNSVGSVPTRLESPTDKLTSFGNIHFDEEV 60

Query: 119 KSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEENCIEFDNKLK 178
           KSKGLSSIK+SIEGNLLFLHLWNDVLV++CVIATLLDPLFCYIL+VD+E++CIEFDNKL 
Sbjct: 61  KSKGLSSIKRSIEGNLLFLHLWNDVLVLMCVIATLLDPLFCYILLVDEEKSCIEFDNKLM 120

Query: 179 VTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTLARRYLLSYFIVDILAVLPLP 238
           VTVVVLRSLVDFGYILLIVFHFR  YTAPNDA+SGRLCTLA RYLLSYF VD+LAVLPLP
Sbjct: 121 VTVVVLRSLVDFGYILLIVFHFRIGYTAPNDASSGRLCTLATRYLLSYFTVDVLAVLPLP 180

Query: 239 QVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKKVRWSSGILPDSAGAKA 298
           QVVIL+V QGTK SHLT A+RSLKFILIFQY PRVSRVY FLKKVRWSSGILPDSAG KA
Sbjct: 181 QVVILIVTQGTKASHLT-ALRSLKFILIFQYFPRVSRVYLFLKKVRWSSGILPDSAGVKA 240

Query: 299 IFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCHRINNNSSFERSCINDFCSR 358
           +FNLFLYMLASH FGAFWYLFSVERKASC+Q+RC+S+PYC R+NNNSSFERSCIND CS 
Sbjct: 241 LFNLFLYMLASHAFGAFWYLFSVERKASCVQIRCNSHPYCSRMNNNSSFERSCINDVCSG 300

Query: 359 KGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQNLRSVFKSLCIMYYYSTP 418
              SN TTA DYGIF+DA NSGVV STDFIWKFSYCCWWGLQNL                
Sbjct: 301 TA-SNVTTALDYGIFDDALNSGVVSSTDFIWKFSYCCWWGLQNL---------------- 360

Query: 419 TLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVLFALLIGN----LQATIAR 478
                        SSLGQGLKTSK IWEIYFA+SITIAGLVLFALLIGN    LQATIAR
Sbjct: 361 -------------SSLGQGLKTSKDIWEIYFAISITIAGLVLFALLIGNLQTYLQATIAR 420

Query: 479 LEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGIDVANILHSLPRDLRRDTT 538
           LEEMRLKGQDIELWMAYHSLPRDLR RIKQYEKYKWRKTRG+DVANIL++LP+DLRRDTT
Sbjct: 421 LEEMRLKGQDIELWMAYHSLPRDLRNRIKQYEKYKWRKTRGVDVANILNNLPKDLRRDTT 480

Query: 539 RHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVREGEPLEEMIFIIHGKLWI 598
           RHLCLRAIKSVSMFQN DEKFLDAVC +LKP LYIERNFIVREGEPL+EMIFIIHGKLWI
Sbjct: 481 RHLCLRAIKSVSMFQNTDEKFLDAVCSYLKPRLYIERNFIVREGEPLDEMIFIIHGKLWI 540

Query: 599 YSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVPISTKTVSTHTKVEAFVLSA 658
           YSN+SRS E SGSSESLTKGDFFGEDLLKWVLKDP LTTVP+STKTVSTHTKVEAFVL+A
Sbjct: 541 YSNSSRSDEISGSSESLTKGDFFGEDLLKWVLKDPLLTTVPMSTKTVSTHTKVEAFVLTA 600

Query: 659 NDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWRRYFKSKREREKSQPSLVIE 718
           NDLKNVVSKFWWLISRELRNDPN KERWA  AAVVLQAAWRRYFK+KREREK + S+V+E
Sbjct: 601 NDLKNVVSKFWWLISRELRNDPNLKERWAAWAAVVLQAAWRRYFKNKREREKCEQSVVVE 660

Query: 719 NGNSQPSATTTTLHASRFIARALHALNQRRKRS-GSNDEPGPSNSNDLQEQSN 767
            GN QP+ATTT+LHASRFIAR + ALN RRK+  GSNDE GPSNSN LQEQSN
Sbjct: 661 IGNPQPAATTTSLHASRFIARIVRALNHRRKKGIGSNDEAGPSNSNGLQEQSN 682

BLAST of Lsi05G000850 vs. NCBI nr
Match: XP_011651606.1 (cyclic nucleotide-gated ion channel 1 isoform X2 [Cucumis sativus] >KAE8650815.1 hypothetical protein Csa_017570 [Cucumis sativus])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 569/687 (82.82%), Postives = 606/687 (88.21%), Query Frame = 0

Query: 85  DIRSTNSMGSVPTRLESSADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVL 144
           DI STNS+GSVPTRLES  D LTSFGNIHFDEEVKSKGLSSIK+SIEGNLLFLHLWNDVL
Sbjct: 5   DIHSTNSVGSVPTRLESPTDKLTSFGNIHFDEEVKSKGLSSIKRSIEGNLLFLHLWNDVL 64

Query: 145 VMLCVIATLLDPLFCYILVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRY 204
           V++CVIATLLDPLFCYIL+VD+E++CIEFDNKL VTVVVLRSLVDFGYILLIVFHFR  Y
Sbjct: 65  VLMCVIATLLDPLFCYILLVDEEKSCIEFDNKLMVTVVVLRSLVDFGYILLIVFHFRIGY 124

Query: 205 TAPNDANSGRLCTLARRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFI 264
           TAPNDA+SGRLCTLA RYLLSYF VD+LAVLPLPQVVIL+V QGTK SHLT A+RSLKFI
Sbjct: 125 TAPNDASSGRLCTLATRYLLSYFTVDVLAVLPLPQVVILIVTQGTKASHLT-ALRSLKFI 184

Query: 265 LIFQYLPRVSRVYSFLKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERK 324
           LIFQY PRVSRVY FLKKVRWSSGILPDSAG KA+FNLFLYMLASH FGAFWYLFSVERK
Sbjct: 185 LIFQYFPRVSRVYLFLKKVRWSSGILPDSAGVKALFNLFLYMLASHAFGAFWYLFSVERK 244

Query: 325 ASCLQVRCHSYPYCHRINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFS 384
           ASC+Q+RC+S+PYC R+NNNSSFERSCIND CS    SN TTA DYGIF+DA NSGVV S
Sbjct: 245 ASCVQIRCNSHPYCSRMNNNSSFERSCINDVCSGTA-SNVTTALDYGIFDDALNSGVVSS 304

Query: 385 TDFIWKFSYCCWWGLQNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHI 444
           TDFIWKFSYCCWWGLQNL                             SSLGQGLKTSK I
Sbjct: 305 TDFIWKFSYCCWWGLQNL-----------------------------SSLGQGLKTSKDI 364

Query: 445 WEIYFAVSITIAGLVLFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRK 504
           WEIYFA+SITIAGLVLFALLIGN    LQATIARLEEMRLKGQDIELWMAYHSLPRDLR 
Sbjct: 365 WEIYFAISITIAGLVLFALLIGNLQTYLQATIARLEEMRLKGQDIELWMAYHSLPRDLRN 424

Query: 505 RIKQYEKYKWRKTRGIDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVC 564
           RIKQYEKYKWRKTRG+DVANIL++LP+DLRRDTTRHLCLRAIKSVSMFQN DEKFLDAVC
Sbjct: 425 RIKQYEKYKWRKTRGVDVANILNNLPKDLRRDTTRHLCLRAIKSVSMFQNTDEKFLDAVC 484

Query: 565 GHLKPMLYIERNFIVREGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGED 624
            +LKP LYIERNFIVREGEPL+EMIFIIHGKLWIYSN+SRS E SGSSESLTKGDFFGED
Sbjct: 485 SYLKPRLYIERNFIVREGEPLDEMIFIIHGKLWIYSNSSRSDEISGSSESLTKGDFFGED 544

Query: 625 LLKWVLKDPHLTTVPISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKE 684
           LLKWVLKDP LTTVP+STKTVSTHTKVEAFVL+ANDLKNVVSKFWWLISRELRNDPN KE
Sbjct: 545 LLKWVLKDPLLTTVPMSTKTVSTHTKVEAFVLTANDLKNVVSKFWWLISRELRNDPNLKE 604

Query: 685 RWAPLAAVVLQAAWRRYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHAL 744
           RWA  AAVVLQAAWRRYFK+KREREK + S+V+E GN QP+ATTT+LHASRFIAR + AL
Sbjct: 605 RWAAWAAVVLQAAWRRYFKNKREREKCEQSVVVEIGNPQPAATTTSLHASRFIARIVRAL 660

Query: 745 NQRRKRS-GSNDEPGPSNSNDLQEQSN 767
           N RRK+  GSNDE GPSNSN LQEQSN
Sbjct: 665 NHRRKKGIGSNDEAGPSNSNGLQEQSN 660

BLAST of Lsi05G000850 vs. NCBI nr
Match: XP_022944855.1 (cyclic nucleotide-gated ion channel 1-like isoform X6 [Cucurbita moschata] >XP_022944857.1 cyclic nucleotide-gated ion channel 1-like isoform X6 [Cucurbita moschata])

HSP 1 Score: 1027.3 bits (2655), Expect = 6.7e-296
Identity = 551/733 (75.17%), Postives = 600/733 (81.86%), Query Frame = 0

Query: 45  WLLENAVQQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNS---MGSVPTRLES 104
           W   + V  +   AMN ED AIRIQ+          SS  DIRS NS   +G+VP R ES
Sbjct: 14  WKDIHPVSGKKEAAMNGEDAAIRIQT----------SSPVDIRSENSTGTVGAVPRRFES 73

Query: 105 SADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYI 164
           S+DTLTSFGNIHF EEVKSKGLSSIK+SIEGN +FLHLWNDVLVMLCVIA LLDPLFCYI
Sbjct: 74  SSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAALLDPLFCYI 133

Query: 165 LVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTL--A 224
           LVVD E+NCI FD KL++T VVLRSL+DFGYILLIVFHFR  YT   DAN+G L T+  A
Sbjct: 134 LVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNGTLSTIATA 193

Query: 225 RRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSF 284
           RRYLLSY  VDILA LPLPQVVI+L  Q  KGSH TI+IR+LKF+LI QYLPRV RVY F
Sbjct: 194 RRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLPRVFRVYLF 253

Query: 285 LKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCH 344
           LKKVRWSSGILPDSA AKAIFNLFLYMLASHVFGAFWYLFS+ERKA+CLQ RCHS+PYC 
Sbjct: 254 LKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSRCHSHPYCP 313

Query: 345 RINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGL 404
           R   N + E SC+ D CSRK  SN T  F++GIF+DAF   VV STDFIWK SYC WWGL
Sbjct: 314 R-KYNITEEGSCL-DNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVSYCYWWGL 373

Query: 405 QNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLV 464
           QNL                             SSLGQGL+TSKHIWEIYFAVSITIAGLV
Sbjct: 374 QNL-----------------------------SSLGQGLRTSKHIWEIYFAVSITIAGLV 433

Query: 465 LFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRG 524
           LFALLIGN    LQ+TIARLEEMRLKGQDIELWMAYHSLPRDLRKRIK YEKYKWRKTRG
Sbjct: 434 LFALLIGNLQTFLQSTIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRG 493

Query: 525 IDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIV 584
           +DVANILH+LPRDLRRDTTRHLCL AIKSVSMFQNMDEKFLDAVCG LKPMLYIERNFIV
Sbjct: 494 VDVANILHNLPRDLRRDTTRHLCLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIV 553

Query: 585 REGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVP 644
           REGEPL+EMIFIIHGKLWIYSN++R GET GSSESL KGD FGE+LLKWVLKDP L+TVP
Sbjct: 554 REGEPLDEMIFIIHGKLWIYSNSNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVP 613

Query: 645 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWR 704
           ISTKTVSTHTKVEAFVLSA+DLKNVVSKFWWLIS+E  NDPNFKERWAPLAA+V+QAAWR
Sbjct: 614 ISTKTVSTHTKVEAFVLSAHDLKNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWR 673

Query: 705 RYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQRRK-RSGSNDEPG 764
           RYFKSKREREKSQ SL  E+GNSQPS  TTT+HASRFIARAL+AL +R+K R+ S+D PG
Sbjct: 674 RYFKSKREREKSQLSLATESGNSQPS-VTTTIHASRFIARALNALKRRQKRRNASSDMPG 704

Query: 765 PSNSNDLQEQSNV 768
            SN+NDL + SN+
Sbjct: 734 TSNNNDLPKHSNI 704

BLAST of Lsi05G000850 vs. NCBI nr
Match: XP_022944856.1 (cyclic nucleotide-gated ion channel 1-like isoform X7 [Cucurbita moschata])

HSP 1 Score: 1026.9 bits (2654), Expect = 8.7e-296
Identity = 551/733 (75.17%), Postives = 600/733 (81.86%), Query Frame = 0

Query: 45  WLLENAVQQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNS---MGSVPTRLES 104
           W   + V  +   AMN ED AIRIQ+          SS  DIRS NS   +G+VP R ES
Sbjct: 14  WKDIHPVSGKKEAAMNGEDAAIRIQT----------SSPVDIRSENSTGTVGAVPRRFES 73

Query: 105 SADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYI 164
           S+DTLTSFGNIHF EEVKSKGLSSIK+SIEGN +FLHLWNDVLVMLCVIA LLDPLFCYI
Sbjct: 74  SSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAALLDPLFCYI 133

Query: 165 LVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTL--A 224
           LVVD E+NCI FD KL++T VVLRSL+DFGYILLIVFHFR  YT   DAN+G L T+  A
Sbjct: 134 LVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNGTLSTIATA 193

Query: 225 RRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSF 284
           RRYLLSY  VDILA LPLPQVVI+L  Q  KGSH TI+IR+LKF+LI QYLPRV RVY F
Sbjct: 194 RRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLPRVFRVYLF 253

Query: 285 LKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCH 344
           LKKVRWSSGILPDSA AKAIFNLFLYMLASHVFGAFWYLFS+ERKA+CLQ RCHS+PYC 
Sbjct: 254 LKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSRCHSHPYCP 313

Query: 345 RINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGL 404
           R   N + E SC+ D CSRK  SN T  F++GIF+DAF   VV STDFIWK SYC WWGL
Sbjct: 314 R-KYNITEEGSCL-DNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVSYCYWWGL 373

Query: 405 QNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLV 464
           QNL                             SSLGQGL+TSKHIWEIYFAVSITIAGLV
Sbjct: 374 QNL-----------------------------SSLGQGLRTSKHIWEIYFAVSITIAGLV 433

Query: 465 LFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRG 524
           LFALLIGN    LQ+TIARLEEMRLKGQDIELWMAYHSLPRDLRKRIK YEKYKWRKTRG
Sbjct: 434 LFALLIGNLQTFLQSTIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRG 493

Query: 525 IDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIV 584
           +DVANILH+LPRDLRRDTTRHLCL AIKSVSMFQNMDEKFLDAVCG LKPMLYIERNFIV
Sbjct: 494 VDVANILHNLPRDLRRDTTRHLCLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIV 553

Query: 585 REGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVP 644
           REGEPL+EMIFIIHGKLWIYSN++R GET GSSESL KGD FGE+LLKWVLKDP L+TVP
Sbjct: 554 REGEPLDEMIFIIHGKLWIYSNSNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVP 613

Query: 645 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWR 704
           ISTKTVSTHTKVEAFVLSA+DLKNVVSKFWWLIS+E  NDPNFKERWAPLAA+V+QAAWR
Sbjct: 614 ISTKTVSTHTKVEAFVLSAHDLKNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWR 673

Query: 705 RYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQRRK-RSGSNDEPG 764
           RYFKSKREREKSQ SL  E+GNSQPS  TTT+HASRFIARAL+AL +R+K R+ S+D PG
Sbjct: 674 RYFKSKREREKSQLSLATESGNSQPS-VTTTIHASRFIARALNALKRRQKRRNASSDMPG 704

Query: 765 PSNSNDLQEQSNV 768
            SN+NDL + SN+
Sbjct: 734 TSNNNDLLKHSNI 704

BLAST of Lsi05G000850 vs. NCBI nr
Match: XP_022944852.1 (cyclic nucleotide-gated ion channel 1-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 1023.5 bits (2645), Expect = 9.6e-295
Identity = 549/727 (75.52%), Postives = 596/727 (81.98%), Query Frame = 0

Query: 45  WLLENAVQQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNS---MGSVPTRLES 104
           W   + V  +   AMN ED AIRIQ+          SS  DIRS NS   +G+VP R ES
Sbjct: 14  WKDIHPVSGKKEAAMNGEDAAIRIQT----------SSPVDIRSENSTGTVGAVPRRFES 73

Query: 105 SADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYI 164
           S+DTLTSFGNIHF EEVKSKGLSSIK+SIEGN +FLHLWNDVLVMLCVIA LLDPLFCYI
Sbjct: 74  SSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAALLDPLFCYI 133

Query: 165 LVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGRLCTL--A 224
           LVVD E+NCI FD KL++T VVLRSL+DFGYILLIVFHFR  YT   DAN+G L T+  A
Sbjct: 134 LVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNGTLSTIATA 193

Query: 225 RRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSF 284
           RRYLLSY  VDILA LPLPQVVI+L  Q  KGSH TI+IR+LKF+LI QYLPRV RVY F
Sbjct: 194 RRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLPRVFRVYLF 253

Query: 285 LKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCH 344
           LKKVRWSSGILPDSA AKAIFNLFLYMLASHVFGAFWYLFS+ERKA+CLQ RCHS+PYC 
Sbjct: 254 LKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSRCHSHPYCP 313

Query: 345 RINNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGL 404
           R   N + E SC+ D CSRK  SN T  F++GIF+DAF   VV STDFIWK SYC WWGL
Sbjct: 314 R-KYNITEEGSCL-DNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVSYCYWWGL 373

Query: 405 QNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLV 464
           QNL                             SSLGQGL+TSKHIWEIYFAVSITIAGLV
Sbjct: 374 QNL-----------------------------SSLGQGLRTSKHIWEIYFAVSITIAGLV 433

Query: 465 LFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRG 524
           LFALLIGN    LQ+TIARLEEMRLKGQDIELWMAYHSLPRDLRKRIK YEKYKWRKTRG
Sbjct: 434 LFALLIGNLQTFLQSTIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRG 493

Query: 525 IDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIV 584
           +DVANILH+LPRDLRRDTTRHLCL AIKSVSMFQNMDEKFLDAVCG LKPMLYIERNFIV
Sbjct: 494 VDVANILHNLPRDLRRDTTRHLCLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIV 553

Query: 585 REGEPLEEMIFIIHGKLWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHLTTVP 644
           REGEPL+EMIFIIHGKLWIYSN++R GET GSSESL KGD FGE+LLKWVLKDP L+TVP
Sbjct: 554 REGEPLDEMIFIIHGKLWIYSNSNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVP 613

Query: 645 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNFKERWAPLAAVVLQAAWR 704
           ISTKTVSTHTKVEAFVLSA+DLKNVVSKFWWLIS+E  NDPNFKERWAPLAA+V+QAAWR
Sbjct: 614 ISTKTVSTHTKVEAFVLSAHDLKNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWR 673

Query: 705 RYFKSKREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQRRK-RSGSNDEPG 762
           RYFKSKREREKSQ SL  E+GNSQPS  TTT+HASRFIARAL+AL +R+K R+ S+D PG
Sbjct: 674 RYFKSKREREKSQLSLATESGNSQPS-VTTTIHASRFIARALNALKRRQKRRNASSDMPG 698

BLAST of Lsi05G000850 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-147
Identity = 315/670 (47.01%), Postives = 419/670 (62.54%), Query Frame = 0

Query: 108 SFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKE 167
           S+ +  F E V SKG+ S  K ++    FL  WN + V+ C+IA  LDPLF Y+ ++D  
Sbjct: 64  SYKSYSFKEAV-SKGIGSTHKILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIIDDA 123

Query: 168 ENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARR 227
           + C+  D K+++T  VLRS  D  Y+L I+F FR  + AP+    GR         +A+R
Sbjct: 124 KKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIAKR 183

Query: 228 YLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLK 287
           YL S+FI+DILAVLPLPQ+VIL++I   +GS        LKFI+ FQY+PR  R+Y   K
Sbjct: 184 YLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNTKNMLKFIVFFQYIPRFIRIYPLYK 243

Query: 288 KVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRC-HSYPYCHR 347
           +V  +SGIL ++A A A FNLFLYMLASHVFGAFWYLFS+ER+  C +  C  + P C  
Sbjct: 244 EVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPCIS 303

Query: 348 ----INNNSSFERSCINDFCSRKGPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCW 407
                +  ++   + +N+ C  + P+  TT FD+GIF DA  SGVV S DF  KF YC W
Sbjct: 304 KLLYCDPETAGGNAFLNESCPIQTPN--TTLFDFGIFLDALQSGVVESQDFPQKFFYCFW 363

Query: 408 WGLQNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIA 467
           WGLQNL                             SSLGQ LKTS +IWEI FAV I+IA
Sbjct: 364 WGLQNL-----------------------------SSLGQNLKTSTYIWEICFAVFISIA 423

Query: 468 GLVLFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRK 527
           GLVLF+ LIGN    LQ+T  RLEEMR+K +D E WM++  LP +LRKRI++YE+YKW++
Sbjct: 424 GLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQE 483

Query: 528 TRGIDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERN 587
           TRG+D  N+L +LP+DLRRD  RHLCL  +  V MF+ MDE+ LDA+C  L+P+LY E +
Sbjct: 484 TRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEES 543

Query: 588 FIVREGEPLEEMIFIIHGK-LWIYSNTSRSGETSGSSESLTKGDFFGEDLLKWVLKDPHL 647
           +IVREG+P++EM+FI+ GK L I +N  R+G    +SE L  GDF GE+LL W L DPH 
Sbjct: 544 YIVREGDPVDEMLFIMRGKLLTITTNGGRTGFL--NSEYLGAGDFCGEELLTWAL-DPHS 603

Query: 648 TT-VPISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRND-PNFKERWAPLAAVV 707
           ++ +PIST+TV    +VEAF L A+DLK V S+F  L S++LR+    + ++W   AA  
Sbjct: 604 SSNLPISTRTVRALMEVEAFALKADDLKFVASQFRRLHSKQLRHTFRYYSQQWKTWAACF 663

Query: 708 LQAAWRRYFKS------KREREKSQPSLVIENGNSQPSATTTTLHASRFIARALHALNQR 754
           +QAAWRRY K       K E  + Q +L  E   S PS    T++ASRF A  L  +   
Sbjct: 664 IQAAWRRYIKKKLEESLKEEENRLQDALAKEACGSSPS-LGATIYASRFAANILRTI--- 693

BLAST of Lsi05G000850 vs. TAIR 10
Match: AT1G01340.1 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 464.2 bits (1193), Expect = 2.1e-130
Identity = 276/658 (41.95%), Postives = 386/658 (58.66%), Query Frame = 0

Query: 113 HFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEENCIE 172
           H D + K     + K  I     FL  WN + +  CV+A  +DPLF YI +VD   +C+ 
Sbjct: 47  HKDPDHKETSSVTRKNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLT 106

Query: 173 FDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARRYLLSY 232
            D+KL++   +LR+L+D  YI+ IVF FR  Y AP+    GR         +A +YL SY
Sbjct: 107 LDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSY 166

Query: 233 FIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKKVRWS 292
           FI+D+L++LPLPQ+V+L VI         +    LKF +I QY+PR+ R+Y    +V  +
Sbjct: 167 FIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRT 226

Query: 293 SGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCHRINNNSS 352
           SGI+ ++A A A +NL LYMLASHVFGA WYL SVER+  C Q  C     C+       
Sbjct: 227 SGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCE 286

Query: 353 FERSCINDFCSRK------GPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQ 412
            +R+  N+F +        G    +T F++GIF DA  SGVV S DF  KF YC WWGL+
Sbjct: 287 NDRNVSNNFLTTSCPFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLR 346

Query: 413 NLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVL 472
           NL                             S+LGQ L+TSK + EI FA+SI I+GLVL
Sbjct: 347 NL-----------------------------SALGQNLQTSKFVGEIIFAISICISGLVL 406

Query: 473 FALLIGNLQ----ATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGI 532
           FALLIGN+Q    +T  R EEMR++ +D E WM++  LP DLRKRI++YE+Y+W++TRG+
Sbjct: 407 FALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGV 466

Query: 533 DVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVR 592
           +   +L +LP+DLRRD  RHLCL  +K V +F+ MDE+ LDAVC  L+P+LY E ++++R
Sbjct: 467 EEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAVCDRLRPVLYTENSYVIR 526

Query: 593 EGEPLEEMIFIIHGKLWIYSNTSRSGETS-GSSESLTKGDFFGEDLLKWVLKDPHLTTVP 652
           EG+P+ EM+F++ G+L   S T+  G +   ++ +L   DF GEDLL W L     +  P
Sbjct: 527 EGDPVGEMLFVMRGRL--VSATTNGGRSGFFNAVNLKASDFCGEDLLPWALDPQSSSHFP 586

Query: 653 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNF-KERWAPLAAVVLQAAW 712
           IST+TV   T+VEAF L+A DLK+V S+F  L S++L++   F   +W   +   +QAAW
Sbjct: 587 ISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFYSVQWRTWSVSFIQAAW 646

Query: 713 RRY-----FKSKREREKSQPSLVIENGNSQPSATTTT-------LHASRFIARALHAL 741
           RRY      KS R+ E      +        +AT ++       L+ASRF + ALH L
Sbjct: 647 RRYCRRKLAKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGALYASRFASNALHNL 673

BLAST of Lsi05G000850 vs. TAIR 10
Match: AT1G01340.2 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 464.2 bits (1193), Expect = 2.1e-130
Identity = 276/658 (41.95%), Postives = 386/658 (58.66%), Query Frame = 0

Query: 113 HFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEENCIE 172
           H D + K     + K  I     FL  WN + +  CV+A  +DPLF YI +VD   +C+ 
Sbjct: 52  HKDPDHKETSSVTRKNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLT 111

Query: 173 FDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARRYLLSY 232
            D+KL++   +LR+L+D  YI+ IVF FR  Y AP+    GR         +A +YL SY
Sbjct: 112 LDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSY 171

Query: 233 FIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKKVRWS 292
           FI+D+L++LPLPQ+V+L VI         +    LKF +I QY+PR+ R+Y    +V  +
Sbjct: 172 FIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRT 231

Query: 293 SGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRCHSYPYCHRINNNSS 352
           SGI+ ++A A A +NL LYMLASHVFGA WYL SVER+  C Q  C     C+       
Sbjct: 232 SGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCE 291

Query: 353 FERSCINDFCSRK------GPSNGTTAFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQ 412
            +R+  N+F +        G    +T F++GIF DA  SGVV S DF  KF YC WWGL+
Sbjct: 292 NDRNVSNNFLTTSCPFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLR 351

Query: 413 NLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVL 472
           NL                             S+LGQ L+TSK + EI FA+SI I+GLVL
Sbjct: 352 NL-----------------------------SALGQNLQTSKFVGEIIFAISICISGLVL 411

Query: 473 FALLIGNLQ----ATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGI 532
           FALLIGN+Q    +T  R EEMR++ +D E WM++  LP DLRKRI++YE+Y+W++TRG+
Sbjct: 412 FALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGV 471

Query: 533 DVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVR 592
           +   +L +LP+DLRRD  RHLCL  +K V +F+ MDE+ LDAVC  L+P+LY E ++++R
Sbjct: 472 EEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAVCDRLRPVLYTENSYVIR 531

Query: 593 EGEPLEEMIFIIHGKLWIYSNTSRSGETS-GSSESLTKGDFFGEDLLKWVLKDPHLTTVP 652
           EG+P+ EM+F++ G+L   S T+  G +   ++ +L   DF GEDLL W L     +  P
Sbjct: 532 EGDPVGEMLFVMRGRL--VSATTNGGRSGFFNAVNLKASDFCGEDLLPWALDPQSSSHFP 591

Query: 653 ISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNF-KERWAPLAAVVLQAAW 712
           IST+TV   T+VEAF L+A DLK+V S+F  L S++L++   F   +W   +   +QAAW
Sbjct: 592 ISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFYSVQWRTWSVSFIQAAW 651

Query: 713 RRY-----FKSKREREKSQPSLVIENGNSQPSATTTT-------LHASRFIARALHAL 741
           RRY      KS R+ E      +        +AT ++       L+ASRF + ALH L
Sbjct: 652 RRYCRRKLAKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGALYASRFASNALHNL 678

BLAST of Lsi05G000850 vs. TAIR 10
Match: AT4G01010.1 (cyclic nucleotide-gated channel 13 )

HSP 1 Score: 453.8 bits (1166), Expect = 2.8e-127
Identity = 281/687 (40.90%), Postives = 394/687 (57.35%), Query Frame = 0

Query: 83  HGDIRSTNSMGSVPTRLESSADTLTSFGNIHFDEEVKSKGLSSIKKSIEGNLLFLHLWND 142
           +G  ++  S+ +V   +        SFG+ H  +   +   ++ K  I     FL  WN 
Sbjct: 23  YGRNKARPSLNTVLKNVRRGLKKPLSFGS-HNKKRDSNSSTTTQKNIINPQGSFLQNWNK 82

Query: 143 VLVMLCVIATLLDPLFCYILVVDKEENCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRF 202
           + +   VIA  +DPLF YI +VD E +C+     L++   VLR+ +D  YI+ IVF FR 
Sbjct: 83  IFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFYIIHIVFQFRT 142

Query: 203 RYTAPNDANSGR------LCTLARRYLLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTI 262
            Y +P+    GR         +A +YL SYFI+D+L++LPLPQ+V+L VI         I
Sbjct: 143 AYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVIPNVNKPVSLI 202

Query: 263 AIRSLKFILIFQYLPRVSRVYSFLKKVRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFW 322
               L  ++  QY+PR+ R+Y    +V  +SGI+ ++A A A +NL LYMLASHVFGA W
Sbjct: 203 TKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASHVFGALW 262

Query: 323 YLFSVERKASCLQVRCHSYP--------YCHRINNNSSFERSCINDFCSRKGPSN--GTT 382
           YL SVER+  C +  C   P        YC   + NSS     +   C    P +   +T
Sbjct: 263 YLISVEREDRCWREACEKIPEVCNFRFLYC---DGNSSVRNDFLTTSCPFINPDDITNST 322

Query: 383 AFDYGIFNDAFNSGVVFSTDFIWKFSYCCWWGLQNLRSVFKSLCIMYYYSTPTLRLHISP 442
            F++GIF DA  SG+V S DF  KF YC WWGL+NL                        
Sbjct: 323 VFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNL------------------------ 382

Query: 443 LPSFFSSLGQGLKTSKHIWEIYFAVSITIAGLVLFALLIGNLQ----ATIARLEEMRLKG 502
                S+LGQ L TSK + EI FAVSI I+GLVLFALLIGN+Q    +T  R EEMR++ 
Sbjct: 383 -----SALGQNLNTSKFVGEIIFAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRK 442

Query: 503 QDIELWMAYHSLPRDLRKRIKQYEKYKWRKTRGIDVANILHSLPRDLRRDTTRHLCLRAI 562
           +D E WM++  LP DLRKRI++YE+YKW++TRG++  N+L +LP+DLRRD  RH CL  +
Sbjct: 443 RDAEQWMSHRMLPDDLRKRIRRYEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLL 502

Query: 563 KSVSMFQNMDEKFLDAVCGHLKPMLYIERNFIVREGEPLEEMIFIIHGKLWIYSNTSRSG 622
           K V +F+ MDE+ LDAVC  LKP+LY E ++ +REG+P+EEM+F++ GKL   S T+  G
Sbjct: 503 KKVPLFEIMDEQLLDAVCDKLKPVLYTENSYAIREGDPVEEMLFVMRGKL--MSATTNGG 562

Query: 623 ETS-GSSESLTKGDFFGEDLLKWVLKDPHLTTVPISTKTVSTHTKVEAFVLSANDLKNVV 682
            T   ++  L   DF GEDLL W L     +  PIST+TV   T+VEAF L+A+DLK V 
Sbjct: 563 RTGFFNAVYLKPSDFCGEDLLTWALDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVA 622

Query: 683 SKFWWLISRELRNDPNF-KERWAPLAAVVLQAAWRRYFKSK------REREKSQPSLVIE 741
           S+F  L S++L++   F   +W    A  +QAAWRR+ + K       E ++ + ++   
Sbjct: 623 SQFRRLHSKQLQHTFRFYSVQWRTWGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKR 674

BLAST of Lsi05G000850 vs. TAIR 10
Match: AT2G46430.1 (cyclic nucleotide gated channel 3 )

HSP 1 Score: 444.1 bits (1141), Expect = 2.2e-124
Identity = 285/734 (38.83%), Postives = 414/734 (56.40%), Query Frame = 0

Query: 52  QQRAHLAMNAEDDAIRIQSFSREHQAEEISSHGDIRSTNSMGSVPTRLESSADTLTSFG- 111
           Q+   +  N  DD      FS +     +SS         M +V    E  ++ + +F  
Sbjct: 5   QRNKFVRFNGNDD-----EFSTKTTRPSVSS--------VMKTVRRSFEKGSEKIRTFKR 64

Query: 112 --NIHFDEEVKSKGLSSIKKSIEGNLLFLHLWNDVLVMLCVIATLLDPLFCYILVVDKEE 171
             ++H ++  ++     I + +  N  +L  WN + ++L V+A   DPLF YI  V  E 
Sbjct: 65  PLSVHSNKNKENNKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPER 124

Query: 172 NCIEFDNKLKVTVVVLRSLVDFGYILLIVFHFRFRYTAPNDANSGR------LCTLARRY 231
            C+  D KL+    V R+ +D  Y++ ++F F   +  P+ +  GR         +A RY
Sbjct: 125 FCLNLDKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRY 184

Query: 232 LLSYFIVDILAVLPLPQVVILLVIQGTKGSHLTIAIRSLKFILIFQYLPRVSRVYSFLKK 291
           L SYF++D+L++LP+PQVV+L ++   +     +A   LK+++  QY+PR++R+Y   K+
Sbjct: 185 LGSYFLIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKE 244

Query: 292 VRWSSGILPDSAGAKAIFNLFLYMLASHVFGAFWYLFSVERKASCLQVRC-------HSY 351
           V  +SG++ ++A A A  NLFLYMLASHVFG+FWYL S+ERK  C +  C       H+Y
Sbjct: 245 VTRTSGLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAY 304

Query: 352 PYCHRINNNSSFERSCINDFCSRKGPS--NGTTAFDYGIFNDAFNSGVVFSTDFIWKFSY 411
            YC     ++   R  +N  C    P     +T F++GIF DA  SGVV S DF  KF Y
Sbjct: 305 LYCSPTGEDN---RLFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFY 364

Query: 412 CCWWGLQNLRSVFKSLCIMYYYSTPTLRLHISPLPSFFSSLGQGLKTSKHIWEIYFAVSI 471
           C WWGL+NL                             S+LGQ LKTS    EI FA+ I
Sbjct: 365 CFWWGLRNL-----------------------------SALGQNLKTSAFEGEIIFAIVI 424

Query: 472 TIAGLVLFALLIGN----LQATIARLEEMRLKGQDIELWMAYHSLPRDLRKRIKQYEKYK 531
            I+GLVLFALLIGN    LQ+T  R+EEMR+K +D E WM++  LP DLRKRI++YE+YK
Sbjct: 425 CISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAEQWMSHRMLPDDLRKRIRKYEQYK 484

Query: 532 WRKTRGIDVANILHSLPRDLRRDTTRHLCLRAIKSVSMFQNMDEKFLDAVCGHLKPMLYI 591
           W++T+G++   +L SLP+DLR+D  RHLCL+ +K V  FQ MD++ LDA+C  LK +LY 
Sbjct: 485 WQETKGVEEEALLSSLPKDLRKDIKRHLCLKLLKKVPWFQAMDDRLLDALCARLKTVLYT 544

Query: 592 ERNFIVREGEPLEEMIFIIHGKLWIYSNTSRSGETS-GSSESLTKGDFFGEDLLKWVLKD 651
           E+++IVREGEP+E+M+FI+ G L   S T+  G T   +S  L  GDF G DLL W L D
Sbjct: 545 EKSYIVREGEPVEDMLFIMRGNL--ISTTTYGGRTGFFNSVDLVAGDFCG-DLLTWAL-D 604

Query: 652 PHLTTVPISTKTVSTHTKVEAFVLSANDLKNVVSKFWWLISRELRNDPNF-KERWAPLAA 711
           P  +  PIS++TV   T+VE F+LSA+DLK V +++  L S++LR+   F   +W   AA
Sbjct: 605 PLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQYRRLHSKQLRHMFRFYSVQWQTWAA 664

Query: 712 VVLQAAWRRYFKSK-----RERE-KSQPSLVIENGNSQPSATTTTLHASRFIARALHALN 756
             +QAAW+R+ + K     RE E K   +L  ++           ++ASRF   A HAL 
Sbjct: 665 CFIQAAWKRHCRRKLSKALREEEGKLHNTLQNDDSGGNKLNLGAAIYASRF---ASHALR 686