Lsi05G000410 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G000410
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	BOI-related E3 ubiquitin-protein ligase 1
Location	chr05: 817624 .. 820268 (-)
RNA-Seq Expression	Lsi05G000410
Synteny	Lsi05G000410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCACACAAAAGGTACAAATCCAATTAAAATAAGAACCTTCATGGTTGGCACAGTTTGCCAAGCCATTGTCCCACAAGAAGAAAGAGAGAGAGAGAGGGAGAGAGAGTATGGGCCATTTACAAACAACTCCAACACAAGACGTGGAGATGGCTACAGAACCACTGCTGCACGCCCATCAACCACTTTTTTTTTGGGGGGGGTTAATTTTCTCCAAAAATAGAGAGAAAATTAGACATGAAAATCAGTTGGGGGCAGATGGGAAATTTAAAAAAAGGAAAAAAAAAAAAGGATTTTTTCAAAGATGAAAGAGAGTCATCCTCGAGAAAAGCCAACACCGATTCCAACCCCACGAGAAGAGAGGAAGAGAAGGAGAGGGAAAGAAAAAGGAGAGCAGAATAATAAGGGCAACAGGGCGCCTCTCTCTTGGACTGATTCGTTTCAATCGCATCTCCTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTATTTTACCTTTCTTTTATTTCATTACTCACTATTTATATCACCACGTAACAGGAGGACACAGCCAGATTTTTAATTGCCATTCAAAAACCAAAACAATTTTTAATTTTACACATAAAAAAAGAAAAAAAGCATCATAAAACCAAAAGGGTAGGTGACAAAGATGGTGAAGAAGGCAAGAATGGTGGTGAAGGTGGAGATGAAAGTGGTGGTGCCCTCTTACCAGACATAAATTCATTTCTTTTCTTTCTTTCTTTCATTCTTTCTTTCTTTCTTTCTTTCTTTCTATATACAAAGTTACAAAACTCCCATTTCTTTTCTCTCTCTTATTGAAGACTCCGTCCGTCTAGTCTAAAGACTTAAGGAGGTGGGAAGAGAAGAGCATCTTCCTTGTCTTTCATGCTTCTCTTATGCTACCTTTTATTATTGCTATCCTTCATTCCCAATCTTCTTCACATTGCTCCATAAACAAACCCACCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTAAACTTACTTTCCCCCACTTTTCTTTCTTATTTTCATCTTCTTTCATACAAATTCCTTTCTCCATGGCTGTTCAGGCTCAATATCCTTCTAATGTTTTGCTATTGAACAGGTATAATATACAGATTTTGTTTTCCCTTTTCTCTACTACTTCAAGTTTTAATAACTCTAATGGGTTTTGGATTTTTGGTTTTGGTTTTAGAAATGGTCAAGAAGGACATGATTATTCATTACAGCCACAACCAGGGGGTTTTCTTGACCAGACTCATATGGTGTTCAACAATAATATAGGTGAGTTAGTTTTTTTTTTTTTTTGGTACATTGTTTCTTAGGATGAATTTTCTTTTTCTTTCTTTAGGTTTTGTTGTTCTATTGTGTTTATGAACATCAAAAGTATTTTGATTAATTTGAGTTGTGAAGCCGACGTACCTTTTAGAAATCAATTAATCAATTGGATTTCTGATGAAAGCAGGAAGCAATTCTCGGAAGAGAGGAAGAGAAATCTCCACTGCCACTACAACCATCGCGGCTCCAATCAATCTATCTGCGTTACAGCAGCCTCGTCCTCCACTTCTTGTTGACCTTGCCAAGCTCCATAACCACCAAAACAATGTCGTCTCCACCGGCCTCCGCCTGTCATCCGGTGATCATCAACAGAACCAACAACCGGCGCAACAGCAACAGCAGCAGCAACAACAACATAACCACAACCACAATCTCGTTCGTCAATCATCATCGGCTTTCTTACCGCTATTAACAGACGATTTCGCCTCAAATTTCAAACGGCAACAAGACGAAATAGACCAATTCCTTCAAGCACAGGTTCCAATTCACTCCCTAACACACAACGAGTTTAATCTTCCATCTTCATTTTGATTTTCTGAAGGGAGTTCCGTCTTCATTTCAAAACAGGAAGAGCAACTACGGCGCACATTAGCAGAGAAAAGGCAAAGACACTACCGTGCGCTTCTCGATGCGGTGGAGGAATCGGTAGCCAGAAGGTTACGAGAGAGGGAAGTGGAGGTCGAGAAAGCCACGCGCCGGAATGCCGAGTTAGAAGCACGCGCCGCCCAACTTAGCGTCGAGGCACAGGTCTGGCAAGCAAAGGCTAGGGCACAGGAAGCCACCGCCGTATCTCTACAGGCACAATTACAACAGGCGATGTCCGGCGGTGGATGCGACGGAGGAGACGGCGGCACCGCCGGGGTGGAAGGACAAGCCGACGACGCGGAGTCGGCTCATATTGACCCGGAACGAGTGGCGAGTTTGGGACCGAGTTGCAAAGCGTGTAGAAAACGGTTGGCGTCGATGTTGCTTTTGCCCTGCCGTCATCTCTGCCTCTGTACAGACTGTGACCAAGTGGCTCAGACTTGCCCGTTATGCCTTTCGAAACGAAGTTCCAGCGTAGAGGTCTATCTCTCCTAAGGCCCAAATCCACGGCCCAATGCAAGTTTCTCAGGCCATTTGGCGGGTTTTTCTTTTTTCCTTTTTTCTTTTTCATAAAATTTTAAAAAAGTATTTTAAGTAATAAAAGAATTACATTTCCCTTTTTATTATTATTATTTTTAGAGAAATTCTCCGAGACTTAATTTTTTTTCCTTTCTTTTGGAATGGCCGTGGAAGTAACGTAAATTGGCCACATTGCGAT

mRNA sequence

CTTTCACACAAAAGGTACAAATCCAATTAAAATAAGAACCTTCATGGTTGGCACAGTTTGCCAAGCCATTGTCCCACAAGAAGAAAGAGAGAGAGAGAGGGAGAGAGAGTATGGGCCATTTACAAACAACTCCAACACAAGACGTGGAGATGGCTACAGAACCACTGCTGCACGCCCATCAACCACTTTTTTTTTGGGGGGGGTTAATTTTCTCCAAAAATAGAGAGAAAATTAGACATGAAAATCAGTTGGGGGCAGATGGGAAATTTAAAAAAAGGAAAAAAAAAAAAGGATTTTTTCAAAGATGAAAGAGAGTCATCCTCGAGAAAAGCCAACACCGATTCCAACCCCACGAGAAGAGAGGAAGAGAAGGAGAGGGAAAGAAAAAGGAGAGCAGAATAATAAGGGCAACAGGGCGCCTCTCTCTTGGACTGATTCGTTTCAATCGCATCTCCTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTATTTTACCTTTCTTTTATTTCATTACTCACTATTTATATCACCACGTAACAGGAGGACACAGCCAGATTTTTAATTGCCATTCAAAAACCAAAACAATTTTTAATTTTACACATAAAAAAAGAAAAAAAGCATCATAAAACCAAAAGGGTAGGTGACAAAGATGGTGAAGAAGGCAAGAATGGTGGTGAAGGTGGAGATGAAAGTGGTGGTGCCCTCTTACCAGACATAAATTCATTTCTTTTCTTTCTTTCTTTCATTCTTTCTTTCTTTCTTTCTTTCTTTCTATATACAAAGTTACAAAACTCCCATTTCTTTTCTCTCTCTTATTGAAGACTCCGTCCGTCTAGTCTAAAGACTTAAGGAGGTGGGAAGAGAAGAGCATCTTCCTTGTCTTTCATGCTTCTCTTATGCTACCTTTTATTATTGCTATCCTTCATTCCCAATCTTCTTCACATTGCTCCATAAACAAACCCACCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTAAACTTACTTTCCCCCACTTTTCTTTCTTATTTTCATCTTCTTTCATACAAATTCCTTTCTCCATGGCTGTTCAGGCTCAATATCCTTCTAATGTTTTGCTATTGAACAGAAATGGTCAAGAAGGACATGATTATTCATTACAGCCACAACCAGGGGGTTTTCTTGACCAGACTCATATGGTGTTCAACAATAATATAGGAAGCAATTCTCGGAAGAGAGGAAGAGAAATCTCCACTGCCACTACAACCATCGCGGCTCCAATCAATCTATCTGCGTTACAGCAGCCTCGTCCTCCACTTCTTGTTGACCTTGCCAAGCTCCATAACCACCAAAACAATGTCGTCTCCACCGGCCTCCGCCTGTCATCCGGTGATCATCAACAGAACCAACAACCGGCGCAACAGCAACAGCAGCAGCAACAACAACATAACCACAACCACAATCTCGTTCGTCAATCATCATCGGCTTTCTTACCGCTATTAACAGACGATTTCGCCTCAAATTTCAAACGGCAACAAGACGAAATAGACCAATTCCTTCAAGCACAGGAAGAGCAACTACGGCGCACATTAGCAGAGAAAAGGCAAAGACACTACCGTGCGCTTCTCGATGCGGTGGAGGAATCGGTAGCCAGAAGGTTACGAGAGAGGGAAGTGGAGGTCGAGAAAGCCACGCGCCGGAATGCCGAGTTAGAAGCACGCGCCGCCCAACTTAGCGTCGAGGCACAGGTCTGGCAAGCAAAGGCTAGGGCACAGGAAGCCACCGCCGTATCTCTACAGGCACAATTACAACAGGCGATGTCCGGCGGTGGATGCGACGGAGGAGACGGCGGCACCGCCGGGGTGGAAGGACAAGCCGACGACGCGGAGTCGGCTCATATTGACCCGGAACGAGTGGCGAGTTTGGGACCGAGTTGCAAAGCGTGTAGAAAACGGTTGGCGTCGATGTTGCTTTTGCCCTGCCGTCATCTCTGCCTCTGTACAGACTGTGACCAAGTGGCTCAGACTTGCCCGTTATGCCTTTCGAAACGAAGTTCCAGCGTAGAGGTCTATCTCTCCTAAGGCCCAAATCCACGGCCCAATGCAAGTTTCTCAGGCCATTTGGCGGGTTTTTCTTTTTTCCTTTTTTCTTTTTCATAAAATTTTAAAAAAGTATTTTAAGTAATAAAAGAATTACATTTCCCTTTTTATTATTATTATTTTTAGAGAAATTCTCCGAGACTTAATTTTTTTTCCTTTCTTTTGGAATGGCCGTGGAAGTAACGTAAATTGGCCACATTGCGAT

Coding sequence (CDS)

ATGGCTGTTCAGGCTCAATATCCTTCTAATGTTTTGCTATTGAACAGAAATGGTCAAGAAGGACATGATTATTCATTACAGCCACAACCAGGGGGTTTTCTTGACCAGACTCATATGGTGTTCAACAATAATATAGGAAGCAATTCTCGGAAGAGAGGAAGAGAAATCTCCACTGCCACTACAACCATCGCGGCTCCAATCAATCTATCTGCGTTACAGCAGCCTCGTCCTCCACTTCTTGTTGACCTTGCCAAGCTCCATAACCACCAAAACAATGTCGTCTCCACCGGCCTCCGCCTGTCATCCGGTGATCATCAACAGAACCAACAACCGGCGCAACAGCAACAGCAGCAGCAACAACAACATAACCACAACCACAATCTCGTTCGTCAATCATCATCGGCTTTCTTACCGCTATTAACAGACGATTTCGCCTCAAATTTCAAACGGCAACAAGACGAAATAGACCAATTCCTTCAAGCACAGGAAGAGCAACTACGGCGCACATTAGCAGAGAAAAGGCAAAGACACTACCGTGCGCTTCTCGATGCGGTGGAGGAATCGGTAGCCAGAAGGTTACGAGAGAGGGAAGTGGAGGTCGAGAAAGCCACGCGCCGGAATGCCGAGTTAGAAGCACGCGCCGCCCAACTTAGCGTCGAGGCACAGGTCTGGCAAGCAAAGGCTAGGGCACAGGAAGCCACCGCCGTATCTCTACAGGCACAATTACAACAGGCGATGTCCGGCGGTGGATGCGACGGAGGAGACGGCGGCACCGCCGGGGTGGAAGGACAAGCCGACGACGCGGAGTCGGCTCATATTGACCCGGAACGAGTGGCGAGTTTGGGACCGAGTTGCAAAGCGTGTAGAAAACGGTTGGCGTCGATGTTGCTTTTGCCCTGCCGTCATCTCTGCCTCTGTACAGACTGTGACCAAGTGGCTCAGACTTGCCCGTTATGCCTTTCGAAACGAAGTTCCAGCGTAGAGGTCTATCTCTCCTAA

Protein sequence

MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTATTTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQQQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLLPCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS

Homology

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BLAST of Lsi05G000410 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.9e-17
Identity = 74/210 (35.24%), Postives = 119/210 (56.67%), Query Frame = 0


Query: 130 RQSSSAFLP--LLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEE 189
           ++ + AF P  L+  +  S  ++Q  EID+F+  Q E LR  L  +++   R L  AV+ 
Sbjct: 86  KRRAIAFSPASLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQN 145

Query: 190 SVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMS 249
           ++ ++L+ ++ E+ +  + N  L+ R   L VE Q+W+  A+  EATA +L++ L+Q ++
Sbjct: 146 AILKKLKAKDEEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLA 205

Query: 250 GGGCDGGDGGTAGVEGQADDAESA--HIDPERV-ASLGPSCKACRKRLASMLLLPCRHLC 309
               D  D     +  +ADDAES+    D   V A +   CK C +  AS+L+LPCRHLC
Sbjct: 206 --QVDDLDAFRRPLVEEADDAESSCGSCDGGDVTAVVNGGCKRCGELTASVLVLPCRHLC 265

Query: 310 LCTDCDQVA--QTCPLCLSKRSSSVEVYLS 333
           LCT C   A  +TCP+C    ++SV V +S
Sbjct: 266 LCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of Lsi05G000410 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-15
Identity = 80/235 (34.04%), Postives = 129/235 (54.89%), Query Frame = 0


Query: 111 PAQQQQQQQQQHNHNHNLVRQSSSAF----LPLLTDDFASNFK-RQQDEIDQFLQAQEEQ 170
           P   ++Q+  Q + ++  V++ S AF      L+  +  S  + +QQ EID+F+  Q E+
Sbjct: 74  PLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINVELVSQIQNQQQSEIDRFVAQQTEK 133

Query: 171 LRRTLAEKRQRHYRALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQ 230
           LR  +  ++Q   R L  AV+  +A++L+E++ E+ +    N  L+ R   L VE Q+W+
Sbjct: 134 LRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQIWR 193

Query: 231 AKARAQEATAVSLQAQLQQAMSGGGCDGGDGGTAGVEGQADDAESA------HIDPERVA 290
             A+  EA A +L+  L Q ++    +     +A VE   DDAES+          E V 
Sbjct: 194 DIAQTNEANANTLRTNLDQVLA--QLETFPTASAVVE---DDAESSCGSCCGDGGGEAVT 253

Query: 291 SLGPSCKACRKRLASMLLLPCRHLCLCTDC--DQVAQTCPLCLSKRSSSVEVYLS 333
           ++G  CK C +R AS+L+LPCRHLCLCT C    + +TCP+C    ++SV V +S
Sbjct: 254 AVGGGCKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of Lsi05G000410 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.3e-14
Identity = 97/359 (27.02%), Postives = 161/359 (44.85%), Query Frame = 0


Query: 25  SLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTATTTIAAPINLSALQQPRPPLLVDLA 84
           +++P  GGF +      NN  G              +  + P + S      PPLL    
Sbjct: 27  TMEPTSGGFCN------NNQTGYG----------VVSPFSVPNHTSTTTTATPPLL---- 86

Query: 85  KLHNHQNNVVSTGLRLSSG----DHQQNQQPAQQQQQQQQQHNHNHNLVR--QSSSAFLP 144
            ++   + + +T    + G    D +    P +++ +   + N++H L++  +SSS    
Sbjct: 87  HVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNA 146

Query: 145 LLTD-------------DFASNFKRQQDEIDQFLQA---QEEQLRRTLAEKRQRHYRALL 204
             T              D +S+  +QQ EID+F+     Q E+++  + EKR+R  R ++
Sbjct: 147 ATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIM 206

Query: 205 DAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQL 264
           +A+E+ + +RLR +E E E+  + N  LE R   LS+E Q+W+  A+  EATA  L+  L
Sbjct: 207 EAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNL 266

Query: 265 QQAMS-------------------------GGGCDGGDGGT----AGVEGQADDAESAHI 324
           +  ++                         G  C GG   T     G+E +A D   A  
Sbjct: 267 EHVLAQVKDVSRGAGLEKNMNEEDDAESCCGSSCGGGGEETVRRRVGLEREAQD--KAER 326

Query: 325 DPERVASLGPSCKACRKRLASMLLLPCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
              R+      C+ C +  + +LLLPCRHLCLC  C     TCP+C S +++SV V +S
Sbjct: 327 RRRRM------CRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVHVNMS 357

[Close]

BLAST of Lsi05G000410 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.9e-14
Identity = 72/261 (27.59%), Postives = 136/261 (52.11%), Query Frame = 0


Query: 94  VSTGLRLSSGDHQQNQQPAQQQQQQQQQ----HNHNHNLVRQSSSAFLPLLTDDFASNFK 153
           V + L  +S ++    +P   +++ +++    +   +  ++++ +  L  L  D +SN +
Sbjct: 76  VDSSLTFNSDNNVDFLRPVSSRKRSREESVVLNPSAYMQIQKNPTDPLMFLGQDLSSNVQ 135

Query: 154 RQQDEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLREREVEVEKATRRNAE 213
           +   +ID+ +    E++R  + EKR+   R +++AVE+ + + LR ++ E+    + N  
Sbjct: 136 QHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLF 195

Query: 214 LEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSG-----------------GGCD 273
           LE +   L VE Q+W+  A++ EAT  +L++ LQQ ++                    C 
Sbjct: 196 LEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCG 255

Query: 274 GGDGGTAGVEGQADDAESAHIDPERVASLGPS-CKACRKRLASMLLLPCRHLCLCTDCDQ 333
             D G +  E      E+   D +++  +G S C++C K  AS+LLLPCRH+CLC+ C  
Sbjct: 256 SNDEGDSEEERWKLAGEAQ--DTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGS 315

[Close]

BLAST of Lsi05G000410 vs. ExPASy TrEMBL
Match: A0A1S3CRF0 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC103503495 PE=4 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.0e-161
Identity = 312/333 (93.69%), Postives = 317/333 (95.20%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHM+FNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMMFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIAAPINLSALQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQ 120
           TT+A PINLS LQ QP PPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN     QQQQQQ
Sbjct: 61  TTVATPINLSPLQHQPHPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN-----QQQQQQ 120

Query: 121 QQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYR 180
           QQHNHNHNLVRQSSSAFLPLLT+DFASNFKRQQ+EIDQFLQAQEEQLRRTLAEKRQRHYR
Sbjct: 121 QQHNHNHNLVRQSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYR 180

Query: 181 ALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240
           ALLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ
Sbjct: 181 ALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240

Query: 241 AQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLLP 300
           AQLQQAMSGGGCDGGDGGT GVEGQADDAESAHIDPERV   GPSCKACRKRLASML+LP
Sbjct: 241 AQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILP 300

Query: 301 CRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           CRHLCLC DCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 CRHLCLCADCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Lsi05G000410 vs. ExPASy TrEMBL
Match: A0A0A0LEI4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G911260 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.2e-160
Identity = 313/333 (93.99%), Postives = 316/333 (94.89%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIAAPINLSALQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQ 120
           TTIA PINLS LQ QP PPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN     QQQQQQ
Sbjct: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN-----QQQQQQ 120

Query: 121 QQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYR 180
           QQHN NHNLVR SSSAFLPLLT+DFASNFKRQQ+EIDQFLQAQEEQLRRTLAEKRQRHYR
Sbjct: 121 QQHNQNHNLVRPSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYR 180

Query: 181 ALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240
           ALLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ
Sbjct: 181 ALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240

Query: 241 AQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLLP 300
           AQLQQAMSGGGCDGGDGGT GVEGQADDAESAHIDPERV   GPSCKACRKRLASML+LP
Sbjct: 241 AQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILP 300

Query: 301 CRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           CRHLCLCTDCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 CRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Lsi05G000410 vs. ExPASy TrEMBL
Match: A0A6J1IE25 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471820 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.9e-153
Identity = 299/334 (89.52%), Postives = 315/334 (94.31%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYSLQ QPGGFLDQTHMVFNNNIG+NSRKRGRE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNIGNNSRKRGREMATST 60

Query: 61  TTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ--QPAQQQQQQ 120
            T A PI+LSA+QQPRPPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQNQ  Q  QQQQQQ
Sbjct: 61  ATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQQQQQQQQ 120

Query: 121 QQQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHY 180
           QQQHNHNHNLVRQSSSAF+ LLTDDFASNFKRQQDEIDQFLQAQEEQLR TLA+KRQRHY
Sbjct: 121 QQQHNHNHNLVRQSSSAFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRHY 180

Query: 181 RALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSL 240
           RALLDAVEESVARRLRE+E EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATA++L
Sbjct: 181 RALLDAVEESVARRLREKEAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATALAL 240

Query: 241 QAQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLL 300
           QAQL+QAMS GGCDGGDGGTAG EGQ DDAESA+IDP+RV  LGPSCKACRKRLAS+LLL
Sbjct: 241 QAQLKQAMS-GGCDGGDGGTAGAEGQTDDAESAYIDPDRVRILGPSCKACRKRLASVLLL 300

Query: 301 PCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           PCRHLCLCT CDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 PCRHLCLCTVCDQVAQTCPLCPSKRSSSVEVYLS 333

[Close]

BLAST of Lsi05G000410 vs. ExPASy TrEMBL
Match: A0A6J1EXW3 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439606 PE=4 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.0e-150
Identity = 296/332 (89.16%), Postives = 308/332 (92.77%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYS Q QPGGFLDQTHMVFNNNIG+NSRKRGRE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIGNNSRKRGREMATST 60

Query: 61  TTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQQ 120
            T A PI+LSA+QQPRPPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQNQ     QQQQQQ
Sbjct: 61  ATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQ---PLQQQQQQ 120

Query: 121 QHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRA 180
           Q NHNHNLV QSSS F+ LLTDDFASNFKRQQDEIDQFLQAQEEQLR TLA+KRQRHYRA
Sbjct: 121 QPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRHYRA 180

Query: 181 LLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQA 240
           LLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAV+LQA
Sbjct: 181 LLDAVEESVARRLRERESEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQA 240

Query: 241 QLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLLPC 300
           QLQQAMS GGCDGGDGGTAG EGQ DDAESAHIDP+RV  LGPSCKACRKRLAS+LLLPC
Sbjct: 241 QLQQAMS-GGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASVLLLPC 300

Query: 301 RHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           RHLCLCTDCDQVA TCPLC SKRSSSVEVYLS
Sbjct: 301 RHLCLCTDCDQVATTCPLCPSKRSSSVEVYLS 328

[Close]

BLAST of Lsi05G000410 vs. ExPASy TrEMBL
Match: A0A6J1E527 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN=LOC111026119 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.1e-147
Identity = 298/335 (88.96%), Postives = 310/335 (92.54%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVF NNIG+NSRKRGREISTA 
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVF-NNIGNNSRKRGREISTA- 60

Query: 61  TTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQQ 120
           +TI APINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ    QQ Q   
Sbjct: 61  STIGAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ---SQQHQHNH 120

Query: 121 QHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRA 180
            HNHNHNLVRQ SS FL LLTD+FASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRA
Sbjct: 121 NHNHNHNLVRQ-SSPFLSLLTDNFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRA 180

Query: 181 LLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQA 240
           LLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQA
Sbjct: 181 LLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQA 240

Query: 241 QLQQAMS--GGGCDGGDGGT-AGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLL 300
           QLQQAMS  GGG DG DGG  AG+EGQADDAESA++DP+RV  LGPSCKACRKR+AS++L
Sbjct: 241 QLQQAMSGGGGGGDGVDGGNLAGIEGQADDAESAYVDPDRVVMLGPSCKACRKRVASVVL 300

Query: 301 LPCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           LPCRHLCLCTDCDQV QTCPLCLS+R+SSVEVYLS
Sbjct: 301 LPCRHLCLCTDCDQVVQTCPLCLSQRNSSVEVYLS 329

[Close]

BLAST of Lsi05G000410 vs. NCBI nr
Match: XP_008465916.1 (PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo])

HSP 1 Score: 577.0 bits (1486), Expect = 1.0e-160
Identity = 312/333 (93.69%), Postives = 317/333 (95.20%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHM+FNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMMFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIAAPINLSALQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQ 120
           TT+A PINLS LQ QP PPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN     QQQQQQ
Sbjct: 61  TTVATPINLSPLQHQPHPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN-----QQQQQQ 120

Query: 121 QQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYR 180
           QQHNHNHNLVRQSSSAFLPLLT+DFASNFKRQQ+EIDQFLQAQEEQLRRTLAEKRQRHYR
Sbjct: 121 QQHNHNHNLVRQSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYR 180

Query: 181 ALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240
           ALLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ
Sbjct: 181 ALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240

Query: 241 AQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLLP 300
           AQLQQAMSGGGCDGGDGGT GVEGQADDAESAHIDPERV   GPSCKACRKRLASML+LP
Sbjct: 241 AQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILP 300

Query: 301 CRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           CRHLCLC DCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 CRHLCLCADCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Lsi05G000410 vs. NCBI nr
Match: XP_004148373.2 (BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN60440.1 hypothetical protein Csa_002624 [Cucumis sativus])

HSP 1 Score: 573.9 bits (1478), Expect = 8.7e-160
Identity = 313/333 (93.99%), Postives = 316/333 (94.89%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIAAPINLSALQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQ 120
           TTIA PINLS LQ QP PPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN     QQQQQQ
Sbjct: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN-----QQQQQQ 120

Query: 121 QQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYR 180
           QQHN NHNLVR SSSAFLPLLT+DFASNFKRQQ+EIDQFLQAQEEQLRRTLAEKRQRHYR
Sbjct: 121 QQHNQNHNLVRPSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYR 180

Query: 181 ALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240
           ALLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ
Sbjct: 181 ALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQ 240

Query: 241 AQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLLP 300
           AQLQQAMSGGGCDGGDGGT GVEGQADDAESAHIDPERV   GPSCKACRKRLASML+LP
Sbjct: 241 AQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILP 300

Query: 301 CRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           CRHLCLCTDCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 CRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Lsi05G000410 vs. NCBI nr
Match: XP_038888350.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida])

HSP 1 Score: 570.1 bits (1468), Expect = 1.3e-158
Identity = 315/334 (94.31%), Postives = 319/334 (95.51%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGF-LDQTHMVFNNNIGSNSRKRGREISTA 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQ QPGGF LDQTHMVFNNNIGSNSRKRGREI+TA
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQQQPGGFILDQTHMVFNNNIGSNSRKRGREIATA 60

Query: 61  TTTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQ 120
           T     PINLSALQQPR PLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN  P QQQQQQQ
Sbjct: 61  T-----PINLSALQQPR-PLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNHPPQQQQQQQQ 120

Query: 121 QQHNHNHNLVRQ-SSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHY 180
           QQHNHNHNLVRQ SSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHY
Sbjct: 121 QQHNHNHNLVRQSSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHY 180

Query: 181 RALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSL 240
           RALLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKA+AQEATAVSL
Sbjct: 181 RALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKAKAQEATAVSL 240

Query: 241 QAQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLL 300
           QAQLQQAMSGGGCDGG+GGTAGVEGQADDAESAHIDPERVA  GPSCKACRKRLASML+L
Sbjct: 241 QAQLQQAMSGGGCDGGEGGTAGVEGQADDAESAHIDPERVAISGPSCKACRKRLASMLIL 300

Query: 301 PCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           PCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS
Sbjct: 301 PCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 328

[Close]

BLAST of Lsi05G000410 vs. NCBI nr
Match: XP_022973264.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima])

HSP 1 Score: 551.2 bits (1419), Expect = 6.1e-153
Identity = 299/334 (89.52%), Postives = 315/334 (94.31%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYSLQ QPGGFLDQTHMVFNNNIG+NSRKRGRE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNIGNNSRKRGREMATST 60

Query: 61  TTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ--QPAQQQQQQ 120
            T A PI+LSA+QQPRPPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQNQ  Q  QQQQQQ
Sbjct: 61  ATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQQQQQQQQ 120

Query: 121 QQQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHY 180
           QQQHNHNHNLVRQSSSAF+ LLTDDFASNFKRQQDEIDQFLQAQEEQLR TLA+KRQRHY
Sbjct: 121 QQQHNHNHNLVRQSSSAFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRHY 180

Query: 181 RALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSL 240
           RALLDAVEESVARRLRE+E EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATA++L
Sbjct: 181 RALLDAVEESVARRLREKEAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATALAL 240

Query: 241 QAQLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLL 300
           QAQL+QAMS GGCDGGDGGTAG EGQ DDAESA+IDP+RV  LGPSCKACRKRLAS+LLL
Sbjct: 241 QAQLKQAMS-GGCDGGDGGTAGAEGQTDDAESAYIDPDRVRILGPSCKACRKRLASVLLL 300

Query: 301 PCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           PCRHLCLCT CDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 PCRHLCLCTVCDQVAQTCPLCPSKRSSSVEVYLS 333

[Close]

BLAST of Lsi05G000410 vs. NCBI nr
Match: XP_023542941.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 550.8 bits (1418), Expect = 7.9e-153
Identity = 299/332 (90.06%), Postives = 310/332 (93.37%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYSLQ QPGGFLDQTHMVFNNNIG+NSRKR RE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNIGNNSRKRAREMATST 60

Query: 61  TTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQQ 120
            T A PI+LSA+QQPRPPLLVDLA LHNHQNNVVSTGLRLSSG+HQQNQ   QQQQQQQQ
Sbjct: 61  ATFATPIDLSAIQQPRPPLLVDLANLHNHQNNVVSTGLRLSSGNHQQNQPLQQQQQQQQQ 120

Query: 121 QHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRA 180
           Q NHNHNLV QSSS F+ LLTDDFASNFKRQQDEIDQFLQAQEEQLR TLA+KRQRHYRA
Sbjct: 121 QPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRHYRA 180

Query: 181 LLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQA 240
           LLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAV+LQA
Sbjct: 181 LLDAVEESVARRLREREEEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQA 240

Query: 241 QLQQAMSGGGCDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACRKRLASMLLLPC 300
           QLQQAMS GGCDGGDGGTAG EGQ DDAESAHIDPERV  LGPSCKACRKRLAS+LLLPC
Sbjct: 241 QLQQAMS-GGCDGGDGGTAGAEGQTDDAESAHIDPERVRILGPSCKACRKRLASVLLLPC 300

Query: 301 RHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           RHLCLCTDCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 RHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 331

[Close]

BLAST of Lsi05G000410 vs. TAIR 10
Match: AT1G32740.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 235.0 bits (598), Expect = 9.0e-62
Identity = 166/345 (48.12%), Postives = 218/345 (63.19%), Query Frame = 0


Query: 1   MAVQAQ-YPSNVLLLN-RNGQEGH--DYSLQPQPGG-FLDQTHMVFNNNIGSNSRKRGRE 60
           MAVQAQ + SN+L LN RNG+E    +++LQ Q  G FLDQT+M+FNN   SN RKR RE
Sbjct: 1   MAVQAQHHSSNLLFLNKRNGKEKEHSNFTLQSQAAGDFLDQTNMLFNNG-SSNQRKRRRE 60

Query: 61  ISTATTTIAAPINLSALQQPRPPLLVDLAKLHNHQ---NNVVSTGLRLSSGDHQQNQQPA 120
            +           L  +Q  + P ++DL+ LHN+    +N+V TGLRL SG         
Sbjct: 61  TNNH--------QLLPMQSHQFPQVIDLSLLHNYNHPPSNMVHTGLRLFSG--------- 120

Query: 121 QQQQQQQQQHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAE 180
              + Q Q+ +H              L  D FA++  RQ +E+D+FL AQ E+LRRTLAE
Sbjct: 121 ---EDQAQKISH--------------LSEDVFAAHINRQSEELDEFLHAQAEELRRTLAE 180

Query: 181 KRQRHYRALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQE 240
           KR+ HY+ALL AVEES+ R+LRE+EVE+E+ATRR+ EL AR +QL  E QVWQ +A+A E
Sbjct: 181 KRKMHYKALLGAVEESLVRKLREKEVEIERATRRHNELVARDSQLRAEVQVWQERAKAHE 240

Query: 241 ATAVSLQAQLQQAMS--GGGCDGGDGGTAGVEG-------QADDAESAHIDPERVASLGP 300
             A SLQ+QLQQA++   GGC       A  EG         DDAES ++DPERV    P
Sbjct: 241 DAAASLQSQLQQAVNQCAGGCVSAQDSRAAEEGLLCTTISGVDDAESVYVDPERVKR--P 300

Query: 301 SCKACRKRLASMLLLPCRHLCLCTDCDQVAQTCPLCLSKRSSSVE 329
           +CKACR+R A++++LPCRHL +C  CD+ A  CPLCL+ R+SSVE
Sbjct: 301 NCKACREREATVVVLPCRHLSICPGCDRTALACPLCLTLRNSSVE 308

[Close]

BLAST of Lsi05G000410 vs. TAIR 10
Match: AT5G47050.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.5e-56
Identity = 158/343 (46.06%), Postives = 204/343 (59.48%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTA- 60
           MAVQA +  + L    NGQEG+D   QP    F    H +   N G ++RKR RE+S+  
Sbjct: 1   MAVQAHHHPSTLFFLNNGQEGND---QPHKSQF----HSI---NAGVDTRKRAREVSSVI 60

Query: 61  -TTTIAAPINLSALQQPRPPLLVDLAKLHNHQN-NVVSTGLRLSSGDHQQNQQPAQQQQQ 120
                AAP+N        PP       +   QN NVVSTGLRLS           ++Q Q
Sbjct: 61  DLDITAAPMN--------PPPQTPPQVIGRRQNPNVVSTGLRLS-----------REQSQ 120

Query: 121 QQQQHNHNHNLVRQSSSAFLPL-LTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQR 180
            Q+Q              FL   +T D A   K Q DE+++FLQ Q EQL+R LAE  +R
Sbjct: 121 NQEQR-------------FLSFPITGDVAGEIKSQTDELNRFLQIQGEQLKRMLAENSER 180

Query: 181 HYRALLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAV 240
           +YR LL   EESV RRLRE+E E+EKATRR+ ELEARA Q+  EA+ WQ +A A+EA A 
Sbjct: 181 NYRELLRTTEESVRRRLREKEAEIEKATRRHVELEARATQIETEARAWQMRAAAREAEAT 240

Query: 241 SLQAQLQQAM---SGGG----CDGGDGGTAGVEGQADDAESAHIDPERVASLGPSCKACR 300
           SLQAQL QA+    GGG     +   G   GV+ +A+DAESA++DP+RV  +GP C+ CR
Sbjct: 241 SLQAQLHQAVVVAHGGGVITTVEPQSGSVDGVD-EAEDAESAYVDPDRVEMIGPGCRICR 300

Query: 301 KRLASMLLLPCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 333
           +R A++L LPCRHL +CT+CD   + CPLCLS ++SSVEV+ S
Sbjct: 301 RRSATVLALPCRHLVMCTECDGSVRICPLCLSTKNSSVEVFYS 300

[Close]

BLAST of Lsi05G000410 vs. TAIR 10
Match: AT4G17680.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.4e-54
Identity = 143/334 (42.81%), Postives = 192/334 (57.49%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MA+QAQ+PS     N NGQE  D SLQPQ   F + T        G +SRKR RE+ ++ 
Sbjct: 18  MAIQAQHPSRFFFNNSNGQEASDCSLQPQDTPFTNFT------KAGVDSRKRSREVYSSA 77

Query: 61  TTIAAPINLSALQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQQ 120
                P        P+P  ++D+ +L     NVVSTGLRL   D  QNQQ          
Sbjct: 78  LMNPPP--------PKPSQVIDITELLQKTPNVVSTGLRLFH-DQSQNQQ---------- 137

Query: 121 QHNHNHNLVRQSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRA 180
                          F   L  D     KRQ+DE+D+F+Q Q E+LRRTLA+ R+R Y  
Sbjct: 138 --------------QFFSSLPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRYVE 197

Query: 181 LLDAVEESVARRLREREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQA 240
           LL A EE V R+LR++E E+EKATRR+AELEAR A +  EA+ WQ +A  +EA   SL A
Sbjct: 198 LLCAAEEIVGRKLRKKEAELEKATRRHAELEARVAHIVEEARNWQLRAATREAEVSSLHA 257

Query: 241 QLQQAMSGGGCDGGDGGTAGVEG----QADDAESAHIDPERVASLGPSCKACRKRLASML 300
            LQQA++          T G +G    +A+DAES ++DPER+  +GPSC+ CR++ A+++
Sbjct: 258 HLQQAIANRLDTAAKQSTFGEDGGDAEEAEDAESVYVDPERIELIGPSCRICRRKSATVM 312

Query: 301 LLPCRHLCLCTDCDQVA-QTCPLCLSKRSSSVEV 330
            LPC+HL LC  CD  A + CP+CL+ ++S VEV
Sbjct: 318 ALPCQHLILCNGCDVGAVRVCPICLAVKTSGVEV 312

[Close]

BLAST of Lsi05G000410 vs. TAIR 10
Match: AT4G17680.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.4e-43
Identity = 115/260 (44.23%), Postives = 157/260 (60.38%), Query Frame = 0


Query: 75  PRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQPAQQQQQQQQQHNHNHNLVRQSSS 134
           P+P  ++D+ +L     NVVSTGLRL   D  QNQQ                        
Sbjct: 6   PKPSQVIDITELLQKTPNVVSTGLRLFH-DQSQNQQ------------------------ 65

Query: 135 AFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLR 194
            F   L  D     KRQ+DE+D+F+Q Q E+LRRTLA+ R+R Y  LL A EE V R+LR
Sbjct: 66  QFFSSLPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRYVELLCAAEEIVGRKLR 125

Query: 195 EREVEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSGGGCDGG 254
           ++E E+EKATRR+AELEAR A +  EA+ WQ +A  +EA   SL A LQQA++       
Sbjct: 126 KKEAELEKATRRHAELEARVAHIVEEARNWQLRAATREAEVSSLHAHLQQAIANRLDTAA 185

Query: 255 DGGTAGVEG----QADDAESAHIDPERVASLGPSCKACRKRLASMLLLPCRHLCLCTDCD 314
              T G +G    +A+DAES ++DPER+  +GPSC+ CR++ A+++ LPC+HL LC  CD
Sbjct: 186 KQSTFGEDGGDAEEAEDAESVYVDPERIELIGPSCRICRRKSATVMALPCQHLILCNGCD 240

Query: 315 QVA-QTCPLCLSKRSSSVEV 330
             A + CP+CL+ ++S VEV
Sbjct: 246 VGAVRVCPICLAVKTSGVEV 240

[Close]

BLAST of Lsi05G000410 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 116.3 bits (290), Expect = 4.6e-26
Identity = 102/324 (31.48%), Postives = 161/324 (49.69%), Query Frame = 0


Query: 31  GGFLDQTHMVFNNNIGSNSR--KRGREISTATTTIAAPINLSALQQPRPPLLVDLAKLHN 90
           G  +D  +   N+N+    R  KRGRE  + +  I         Q+ +  L + L   +N
Sbjct: 40  GCTVDPVNYFANDNLVPMIRPNKRGREAESISNNI---------QRQQQKLQMSLNYNYN 99

Query: 91  H--------QNNVVSTGLRLSSGDHQQNQQPAQQQQQQQQQHNHNHNLVRQSSSAFLPL- 150
           +        + N+VSTGLRLS  D                +HN +      S  A  P+ 
Sbjct: 100 NTSVREEVPKENLVSTGLRLSYDD---------------DEHNSSVTSASGSILAASPIF 159

Query: 151 --LTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLRERE 210
             L D    +  RQ+DE DQF++ Q  Q+ + + + +QRH  + L  +E+ V+++L+E++
Sbjct: 160 QSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIASFLTTLEKGVSKKLQEKD 219

Query: 211 VEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSGGG--CDGGD 270
            E+    ++N EL  R  Q+++EAQ W  +A+  E+    L+A LQQAMS         D
Sbjct: 220 HEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKANLQQAMSHNNSVIAAAD 279

Query: 271 GGTAGV-EGQADDAESAHIDPERVASLGPS------CKACRKRLASMLLLPCRHLCLCTD 330
            G  G  + + DDA S++IDP    +          CK C  +  S+L++PCRHL LC +
Sbjct: 280 QGKEGFGDSEIDDAASSYIDPNNNNNNNMGIHQRMRCKMCNVKEVSVLIVPCRHLSLCKE 339

Query: 331 CDQVAQTCPLCLSKRSSSVEVYLS 333
           CD   + CP+C S +SS V+V+ S
Sbjct: 340 CDVFTKICPVCKSLKSSCVQVFFS 339

The following BLAST results are available for this feature:

BLAST of Lsi05G000410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHE4	2.9e-17	35.24	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
O81851	1.2e-15	34.04	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
F4IDI6	2.3e-14	27.02	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9LDD1	8.9e-14	27.59	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Lsi05G000410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CRF0	5.0e-161	93.69	BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC10350349...	[more]
A0A0A0LEI4	4.2e-160	93.99	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G911260 P...	[more]
A0A6J1IE25	2.9e-153	89.52	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1EXW3	3.0e-150	89.16	BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita moschata ...	[more]
A0A6J1E527	4.1e-147	88.96	BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Lsi05G000410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008465916.1	1.0e-160	93.69	PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo]	[more]
XP_004148373.2	8.7e-160	93.99	BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN60440.1 hypothet...	[more]
XP_038888350.1	1.3e-158	94.31	BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida]	[more]
XP_022973264.1	6.1e-153	89.52	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima]	[more]
XP_023542941.1	7.9e-153	90.06	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi05G000410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32740.1	9.0e-62	48.12	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G47050.1	1.5e-56	46.06	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G17680.1	2.4e-54	42.81	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G17680.2	1.4e-43	44.23	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G10650.1	4.6e-26	31.48	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 193..227
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 284..322 e-value: 1.7E-7 score: 31.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 99..125
None	No IPR available	PANTHER	PTHR42647:SF30	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1-LIKE	coord: 1..331
IPR017066	E3 ubiquitin-protein ligase BOI-like	PIRSF	PIRSF036836	SBP1_RNase_bind	coord: 1..332 e-value: 1.4E-88 score: 295.8
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..331
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 263..332 e-value: 4.3E-11 score: 44.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 285..319 score: 9.671455

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G000410.1	Lsi05G000410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity