Lsi04G024150 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G024150
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	1-aminocyclopropane-1-carboxylate oxidase
Location	chr04: 31289051 .. 31291085 (+)
RNA-Seq Expression	Lsi04G024150
Synteny	Lsi04G024150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGTTTTAAAAGAAAAGGGGATTTAATTACTACTAAAGGGTTGACATCCAAGTTGGAGGGAAAGTCTTATAAATAGGGGCAGCCCGTTCCTTTATAAACCCATCAACATAAACTACTCTCTCCCTCATCAACTTCATCCCTTTAATCCATTTTTCTCCCTAAAAGTAACACTGTTTTCAACTTCTAATGGCAATTCCAGTCATTGATTTCTCTAAGCTTGATGGAGAAGAAAGGGCCAAGACATTGGATCAAATAGCGAATGGCTGTGAAGAATGGGGATTCTTTCAGGTAACTTCCGACCTATTATTAGTTATTCTACTCATTTCAGGTTGATGTGAACGATATTTTTTTTTTCATATTCAAAATCAATATGAAACATGATAATTCATCATTTGAATGATTAAATGTATGTTTTAAAATGATTTTGGACATGACATGTTTGAAGTATATGACTTTTTAATACCTAGTTTGGTAACCATTTGGTTTTTGAAAATTAAGCCTATAAACACCACTTTCATCTATTAGTTTTTATGTTTTGTTTGTTTACATTTTAGAAGTATTTTTAAAATCAAAGCCAAAATTTGAAAACAAAACAAAACAATAAAAGAAAAAAGTTCTCAAAAACCTTTTGTTTTTGAAAATTGGCTAAGAATTCAAAAGTTCAATTTTCAAAAACTAAAAACAAAGAACCAAATAGTTACCAAACATAGCCTTTTTTTTTATTATTTTTTATTATGGTTGACTAAATAAATATATGTTGAATATCATCAGCTGGTGAACCATGGGATTCCTGAAGAGCTCCTAGAGAGGGTGAAGCGGATTTCCTCAGAGTGCTACAAGGTGGAAAGGGAAGAAAGTTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTGGAGAACAAGAGTGGTGAGAAGCTGGAAAATCTTGATTGGGAAGACGTCTTCCTTCTCCATGATAACAACGAATGGCCATCCAACATTCCTGGATTCAAGTAAGCCAGCCATCTACACTCCAAACTTTCATGAAAAATCAGTGAAAATATATCATGAAATCACATTTTTCTTACAAACAGGAAAAGAATTAATGTTTATGAATCCAATTGTGGCAGGGAAACCATGAGAGAGTACAGATCGGAACTGAAGAAGCTAGCTGAAAAAGTGATGGCAGTGATGGATGAGAACTTAGGTTTACCTGAAGGATACATAAAGGCTGCCTTCAATGGTGGCGAAGGGCTGGAAAAGGCCTTCTTCGGCACCAAAGTCAGCCACTACCCACCATGCCCACACCCGGAGCTGGTGAATGGCCTCCGTGCCCACACTGATGCCGGTGGTGTCATCTTGCTCTTCCAAGACGACGAGGTGGGAGGCCTTCAAATCCTGAAAGATGGGCAGTGGATAGATGTCCAGCCATTACCCAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGTAATGGCAGATACAAGAGTGTTTGGCACCGGGTTTTAGCCACCCCAAATGGGAACAGGAGATCGATTGCTTCGTTTTACAACCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAGGTGGAAGAAGGATATCCCAAGTTTGTGTTTGGAGACTACATGTCAGTTTATGCTGAGCAGAAGTTCCTCCCAAAGGGTCCAAGGTTTCAAGCTGTCAGGGCAATGTGAAGAGCATGAATCAAAAGGAGTTACTGTTAGATTTAACTCTCCTGATGGGCTTTCATTCAAGGGTTTTCATATAGTAGTGACAAGATTTGAATAATGCTACTTCTTTCCACACCCCCCTCCCCTCACAAAAAGAAAGAAAGAAAAAAAGGAACCACTTTCCCTTTAGGGTTTGTGTGTGTTTGCTTTAGTAGTTTGCTGTTGTTTCTCCATTTATTGTTGTTGTATGTTATGGAGTGTGTCCGAAGGACTAATAATATTCAGTATTGAGTATTGTTTTTCAATATTATATTTTGAAGTTTAAAGATCAAGGTTTATTTT

mRNA sequence

CTTGTTTTAAAAGAAAAGGGGATTTAATTACTACTAAAGGGTTGACATCCAAGTTGGAGGGAAAGTCTTATAAATAGGGGCAGCCCGTTCCTTTATAAACCCATCAACATAAACTACTCTCTCCCTCATCAACTTCATCCCTTTAATCCATTTTTCTCCCTAAAAGTAACACTGTTTTCAACTTCTAATGGCAATTCCAGTCATTGATTTCTCTAAGCTTGATGGAGAAGAAAGGGCCAAGACATTGGATCAAATAGCGAATGGCTGTGAAGAATGGGGATTCTTTCAGCTCCTAGAGAGGGTGAAGCGGATTTCCTCAGAGTGCTACAAGGTGGAAAGGGAAGAAAGTTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTGGAGAACAAGAGTGGTGAGAAGCTGGAAAATCTTGATTGGGAAGACGTCTTCCTTCTCCATGATAACAACGAATGGCCATCCAACATTCCTGGATTCAAGGAAACCATGAGAGAGTACAGATCGGAACTGAAGAAGCTAGCTGAAAAAGTGATGGCAGTGATGGATGAGAACTTAGGTTTACCTGAAGGATACATAAAGGCTGCCTTCAATGGTGGCGAAGGGCTGGAAAAGGCCTTCTTCGGCACCAAAGTCAGCCACTACCCACCATGCCCACACCCGGAGCTGGTGAATGGCCTCCGTGCCCACACTGATGCCGGTGGTGTCATCTTGCTCTTCCAAGACGACGAGGTGGGAGGCCTTCAAATCCTGAAAGATGGGCAGTGGATAGATGTCCAGCCATTACCCAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGTAATGGCAGATACAAGAGTGTTTGGCACCGGGTTTTAGCCACCCCAAATGGGAACAGGAGATCGATTGCTTCGTTTTACAACCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAGGTGGAAGAAGGATATCCCAAGTTTGTGTTTGGAGACTACATGTCAGTTTATGCTGAGCAGAAGTTCCTCCCAAAGGGTCCAAGGTTTCAAGCTGTCAGGGCAATGTGAAGAGCATGAATCAAAAGGAGTTACTGTTAGATTTAACTCTCCTGATGGGCTTTCATTCAAGGGTTTTCATATAGTAGTGACAAGATTTGAATAATGCTACTTCTTTCCACACCCCCCTCCCCTCACAAAAAGAAAGAAAGAAAAAAAGGAACCACTTTCCCTTTAGGGTTTGTGTGTGTTTGCTTTAGTAGTTTGCTGTTGTTTCTCCATTTATTGTTGTTGTATGTTATGGAGTGTGTCCGAAGGACTAATAATATTCAGTATTGAGTATTGTTTTTCAATATTATATTTTGAAGTTTAAAGATCAAGGTTTATTTT

Coding sequence (CDS)

ATGGCAATTCCAGTCATTGATTTCTCTAAGCTTGATGGAGAAGAAAGGGCCAAGACATTGGATCAAATAGCGAATGGCTGTGAAGAATGGGGATTCTTTCAGCTCCTAGAGAGGGTGAAGCGGATTTCCTCAGAGTGCTACAAGGTGGAAAGGGAAGAAAGTTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTGGAGAACAAGAGTGGTGAGAAGCTGGAAAATCTTGATTGGGAAGACGTCTTCCTTCTCCATGATAACAACGAATGGCCATCCAACATTCCTGGATTCAAGGAAACCATGAGAGAGTACAGATCGGAACTGAAGAAGCTAGCTGAAAAAGTGATGGCAGTGATGGATGAGAACTTAGGTTTACCTGAAGGATACATAAAGGCTGCCTTCAATGGTGGCGAAGGGCTGGAAAAGGCCTTCTTCGGCACCAAAGTCAGCCACTACCCACCATGCCCACACCCGGAGCTGGTGAATGGCCTCCGTGCCCACACTGATGCCGGTGGTGTCATCTTGCTCTTCCAAGACGACGAGGTGGGAGGCCTTCAAATCCTGAAAGATGGGCAGTGGATAGATGTCCAGCCATTACCCAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGTAATGGCAGATACAAGAGTGTTTGGCACCGGGTTTTAGCCACCCCAAATGGGAACAGGAGATCGATTGCTTCGTTTTACAACCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAGGTGGAAGAAGGATATCCCAAGTTTGTGTTTGGAGACTACATGTCAGTTTATGCTGAGCAGAAGTTCCTCCCAAAGGGTCCAAGGTTTCAAGCTGTCAGGGCAATGTGA

Protein sequence

MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQLLERVKRISSECYKVEREESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKGPRFQAVRAM

Homology

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BLAST of Lsi04G024150 vs. ExPASy Swiss-Prot
Match: Q0WPW4 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g77330 PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 1.4e-127
Identity = 226/313 (72.20%), Postives = 257/313 (82.11%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+GEER KTL +IA  CEEWGFFQ         LL +VK++SS+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL--HDNNEWPSNIPGFKETMREYRS 120
           EE+FK+S PVKLLN+L++  SGEKLEN+DWEDVF L  H+ NEWPSNI   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELKKLAEKVMAVMDENLGLPEGYIKAAFNGG--EGLEKAFFGTKVSHYPPCPHPELVNGL 180
           E++KLA K+M VMDENLGLP+GYIK AFN G  +G E AFFGTKVSHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDDE  GLQ+LKDG+WIDVQPLPN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKF 300
           RVLA   GNRRSIASFYNPS +A I PAA   ++ +   E+ YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

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BLAST of Lsi04G024150 vs. ExPASy Swiss-Prot
Match: Q9FR99 (1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.8e-122
Identity = 218/311 (70.10%), Postives = 259/311 (83.28%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKLDG+ERA+T+ +IANGCEEWGFFQ         LLERVK++SSECYK+ R
Sbjct: 1   MAIPVIDFSKLDGKERAETMARIANGCEEWGFFQLVNHGIPVELLERVKKVSSECYKL-R 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EE F+ SKPV+LL+ L++   G++L+N+DWEDVF+L D+NEWPSN P F+ETM+EYR E+
Sbjct: 61  EERFEGSKPVQLLDTLVKEGDGQRLDNVDWEDVFVLQDDNEWPSNPPDFEETMKEYREEI 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLAEK+M VMDENLG  +G IK AF+ G+G    FFGTKVSHYPPCP  +LV GLRAHT
Sbjct: 121 RKLAEKMMEVMDENLGFEKGCIKKAFS-GDGQHPPFFGTKVSHYPPCPRLDLVKGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQ+LKDG+WIDVQPL ++IVINTGDQIEVLSNGRYKS WHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQMLKDGRWIDVQPLADAIVINTGDQIEVLSNGRYKSAWHRVLA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEG--YPKFVFGDYMSVYAEQKFLP 300
           T +GNRRSIASFYNPS++ATIAPAA     A +E      YPKF+FGDYM VYA+QK+ P
Sbjct: 241 TSHGNRRSIASFYNPSLKATIAPAA---GAATEEAAPPALYPKFLFGDYMDVYAKQKYEP 300

[Close]

BLAST of Lsi04G024150 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.1e-84
Identity = 158/313 (50.48%), Postives = 215/313 (68.69%), Query Frame = 0


Query: 2   AIPVIDFSKLDGEERAKTLDQIANGCEEWGFF---------QLLERVKRISSECYKVERE 61
           A PVID  KL+GEERA T+++I + CE WGFF         +L++ V+R++ E Y    E
Sbjct: 3   AFPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCME 62

Query: 62  ESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DNNEWPSNIPGFKETMREY 121
           + FK     K L  +       ++ +LDWE  F L      + +E P      ++ M+E+
Sbjct: 63  QRFKEMVATKGLEAV-----QSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEF 122

Query: 122 RSELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGL 181
             +L+KLAE+++ ++ EN+GL +GY+K AF G +G     FGTKVS+YPPCP PEL+ GL
Sbjct: 123 AEKLEKLAEQLLDLLCENVGLEKGYLKKAFYGSKG---PTFGTKVSNYPPCPRPELIKGL 182

Query: 182 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQD++V GLQ+LKDG+WIDV P+ +SIVIN GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMH 242

Query: 242 RVLATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKF 301
           RV+A P+GNR SIASFYNP  +A + PA  LVDK   + ++ YPKFVF DYM +YA  KF
Sbjct: 243 RVIAQPDGNRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKF 302

[Close]

BLAST of Lsi04G024150 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 2.6e-81
Identity = 152/310 (49.03%), Postives = 215/310 (69.35%), Query Frame = 0


Query: 4   PVIDFSKLDGEERAKTLDQIANGCEEWGFF---------QLLERVKRISSECYKVEREES 63
           PV+D S ++GEERA TL++I + CE WGFF         +LL+ V++++ + YK   E+ 
Sbjct: 5   PVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQR 64

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DNNEWPSNIPGFKETMREYRS 123
           FK     K L+D+       ++ +LDWE  F L      + +E P     +++TM+E+  
Sbjct: 65  FKEMVAAKGLDDV-----QSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFAV 124

Query: 124 ELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRA 183
           EL+KLAEK++ ++ ENLGL +GY+K  F G +G     FGTKVS+YPPCP P+L+ GLRA
Sbjct: 125 ELEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPN---FGTKVSNYPPCPKPDLIKGLRA 184

Query: 184 HTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           H+DAGG+ILLFQDD+V GLQ+LKDG+W+DV P+ +SIVIN GDQIEV++NG+YKSV HRV
Sbjct: 185 HSDAGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKYKSVMHRV 244

Query: 244 LATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLP 300
           +A  +G R SIASFYNP  ++ I+PA  +++K  ++    YPKFVF DYM +Y+  KF  
Sbjct: 245 IAQSDGTRMSIASFYNPGNDSFISPAPAVLEKKTEDAPT-YPKFVFDDYMKLYSGLKFQA 304

[Close]

BLAST of Lsi04G024150 vs. ExPASy Swiss-Prot
Match: O48882 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.7e-80
Identity = 153/310 (49.35%), Postives = 212/310 (68.39%), Query Frame = 0


Query: 4   PVIDFSKLDGEERAKTLDQIANGCEEWGFF---------QLLERVKRISSECYKVEREES 63
           PV+D   ++GEERA TL++I + CE WGFF         +LL+ V++++ + YK   E+ 
Sbjct: 5   PVVDMDLINGEERAATLEKINDACENWGFFELVNHGISTELLDTVEKMNKDHYKKTMEQR 64

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DNNEWPSNIPGFKETMREYRS 123
           FK     K L  +       ++  LDWE  F L      + +E P     +++TM+E+  
Sbjct: 65  FKEMVAAKGLEAV-----QSEIHYLDWESTFFLRHLPSSNISEIPDLEEDYRKTMKEFAV 124

Query: 124 ELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRA 183
           EL+KLAEK++ ++ ENLGL +GY+K AF G +G     FGTKVS+YPPCP P+L+ GLRA
Sbjct: 125 ELEKLAEKLLDLLCENLGLEKGYLKKAFYGSKGPN---FGTKVSNYPPCPKPDLIKGLRA 184

Query: 184 HTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           HTDAGG+ILLFQDD+V GLQ+LKDG+W+DV P+ +SIVIN GDQIEV++NG+YKS+ HRV
Sbjct: 185 HTDAGGIILLFQDDKVSGLQLLKDGEWMDVPPVHHSIVINLGDQIEVITNGKYKSIMHRV 244

Query: 244 LATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLP 300
           +A  +G R SIASFYNP  +A I+PA  L+++   EV   YPKF+F DYM +Y+  KF  
Sbjct: 245 IAQSDGTRMSIASFYNPGDDAFISPAPALLEE-KSEVSPTYPKFLFDDYMKLYSGLKFQA 304

[Close]

BLAST of Lsi04G024150 vs. ExPASy TrEMBL
Match: A0A0A0L1U8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361270 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.0e-165
Identity = 287/309 (92.88%), Postives = 297/309 (96.12%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+G+ERAKTL QIANGCEEWGFFQ         LLERVK++SSECYKVER
Sbjct: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           +PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. ExPASy TrEMBL
Match: A0A5A7UFF3 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00040 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.2e-165
Identity = 288/309 (93.20%), Postives = 296/309 (95.79%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+GEERAKTL QIANGCEEWGFFQ         LLERVK++SSECYKVER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMAVMDENLGLPEGYIKAAFN GEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           +PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. ExPASy TrEMBL
Match: A0A1S3B9W8 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103487610 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.2e-165
Identity = 288/309 (93.20%), Postives = 296/309 (95.79%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+GEERAKTL QIANGCEEWGFFQ         LLERVK++SSECYKVER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMAVMDENLGLPEGYIKAAFN GEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           +PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. ExPASy TrEMBL
Match: A0A6J1FFA0 (1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443415 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 8.8e-165
Identity = 287/309 (92.88%), Postives = 296/309 (95.79%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKLDGEERAKTL+QIANGCEEWGFFQ         LLERVKR+ SECYK+ER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMA+MDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           TPNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. ExPASy TrEMBL
Match: A0A6J1IKI6 (1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111477086 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 286/309 (92.56%), Postives = 295/309 (95.47%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKLDGEERAKTL+QIANGCEEWGFFQ         LLERVKR+ SECYK+ER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESF SSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETM EYRSEL
Sbjct: 61  EESFMSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMA+MDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           TPNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. NCBI nr
Match: XP_038898758.1 (1-aminocyclopropane-1-carboxylate oxidase 5 [Benincasa hispida])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 293/309 (94.82%), Postives = 296/309 (95.79%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+GEER KTL QIANGCEEWGFFQ         LLERVK+ISSE YKVER
Sbjct: 1   MAIPVIDFSKLNGEERPKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKISSEYYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+GYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. NCBI nr
Match: XP_004142637.1 (1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis sativus] >XP_031745860.1 1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucumis sativus] >KAE8637421.1 hypothetical protein CSA_021607 [Cucumis sativus] >KGN54542.1 hypothetical protein Csa_012615 [Cucumis sativus])

HSP 1 Score: 590.9 bits (1522), Expect = 6.2e-165
Identity = 287/309 (92.88%), Postives = 297/309 (96.12%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+G+ERAKTL QIANGCEEWGFFQ         LLERVK++SSECYKVER
Sbjct: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           +PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. NCBI nr
Match: XP_008444197.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo] >KAA0052209.1 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo var. makuwa] >TYK01776.1 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 590.1 bits (1520), Expect = 1.1e-164
Identity = 288/309 (93.20%), Postives = 296/309 (95.79%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+GEERAKTL QIANGCEEWGFFQ         LLERVK++SSECYKVER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMAVMDENLGLPEGYIKAAFN GEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           +PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. NCBI nr
Match: KAG7024737.1 (1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 589.3 bits (1518), Expect = 1.8e-164
Identity = 287/309 (92.88%), Postives = 296/309 (95.79%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKLDGEERAKTL+QIANGCEEWGFFQ         LLERVKR+ SECYK+ER
Sbjct: 31  MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 90

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETM EYRSEL
Sbjct: 91  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 150

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMA+MDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 151 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 210

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 211 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 270

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           TPNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 271 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 330

[Close]

BLAST of Lsi04G024150 vs. NCBI nr
Match: XP_022936989.1 (1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 [Cucurbita moschata] >XP_022936990.1 1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X2 [Cucurbita moschata] >KAG6591869.1 1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 589.3 bits (1518), Expect = 1.8e-164
Identity = 287/309 (92.88%), Postives = 296/309 (95.79%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKLDGEERAKTL+QIANGCEEWGFFQ         LLERVKR+ SECYK+ER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAEKVMA+MDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKG 300
           TPNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPK 
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

[Close]

BLAST of Lsi04G024150 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 457.2 bits (1175), Expect = 1.0e-128
Identity = 226/313 (72.20%), Postives = 257/313 (82.11%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           MAIPVIDFSKL+GEER KTL +IA  CEEWGFFQ         LL +VK++SS+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL--HDNNEWPSNIPGFKETMREYRS 120
           EE+FK+S PVKLLN+L++  SGEKLEN+DWEDVF L  H+ NEWPSNI   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELKKLAEKVMAVMDENLGLPEGYIKAAFNGG--EGLEKAFFGTKVSHYPPCPHPELVNGL 180
           E++KLA K+M VMDENLGLP+GYIK AFN G  +G E AFFGTKVSHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDDE  GLQ+LKDG+WIDVQPLPN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKF 300
           RVLA   GNRRSIASFYNPS +A I PAA   ++ +   E+ YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

[Close]

BLAST of Lsi04G024150 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 293.9 bits (751), Expect = 1.5e-79
Identity = 148/315 (46.98%), Postives = 219/315 (69.52%), Query Frame = 0


Query: 2   AIPVIDFSKLDGEERAKTLDQIANGCEEWGFF---------QLLERVKRISSECYKVERE 61
           + P+I+  KL+GEERA T+++I + CE WGFF         +LL++V++++ E YK   E
Sbjct: 3   SFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCME 62

Query: 62  ESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-----HDNNEWPSNIPGFKETMREY 121
           E FK S   + L+ L       ++ ++DWE  F L      + ++ P     ++  M+++
Sbjct: 63  ERFKESIKNRGLDSL-----RSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDF 122

Query: 122 RSELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGL 181
             +++KL+E+++ ++ ENLGL +GY+K  F G    ++  FGTKVS+YPPCP+P+LV GL
Sbjct: 123 AGKIEKLSEELLDLLCENLGLEKGYLKKVFYGS---KRPTFGTKVSNYPPCPNPDLVKGL 182

Query: 182 RAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQDD+V GLQ+LKDG+W+DV P+ +SIV+N GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEH 242

Query: 242 RVLATPNG-NRRSIASFYNPSMEATIAPAAQLVDK-ANQEVEEGYPKFVFGDYMSVYAEQ 301
           RVL+  +G  R SIASFYNP  ++ I PA +L+ K A +E +E YP+FVF DYM +Y+  
Sbjct: 243 RVLSQTDGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAV 302

[Close]

BLAST of Lsi04G024150 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 293.5 bits (750), Expect = 1.9e-79
Identity = 149/316 (47.15%), Postives = 209/316 (66.14%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVER 60
           M  PV+D SKL+GEER +T+  I   CE WGFF+         L++++++++ + YK  +
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  EESFKSSKPVKLLNDLLENKSGEKL----ENLDWEDVFLLH-----DNNEWPSNIPGFKE 120
           E+ F         ND+L++K  + L    E++DWE  F +      + N+       ++ 
Sbjct: 65  EQKF---------NDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRT 124

Query: 121 TMREYRSELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPE 180
            M+++   L+ LAE ++ ++ ENLGL +GY+K  F+G +G     FGTKVS+YPPCP PE
Sbjct: 125 AMKDFGKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKG---PTFGTKVSNYPPCPKPE 184

Query: 181 LVNGLRAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRY 240
           ++ GLRAHTDAGG+ILLFQDD+V GLQ+LKDG WIDV PL +SIVIN GDQ+EV++NG+Y
Sbjct: 185 MIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKY 244

Query: 241 KSVWHRVLATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVY 299
           KSV HRV+    GNR S+ASFYNP  +A I+PA  LV+K ++     YP FVF DYM +Y
Sbjct: 245 KSVLHRVVTQQEGNRMSVASFYNPGSDAEISPATSLVEKDSE-----YPSFVFDDYMKLY 303

[Close]

BLAST of Lsi04G024150 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 9.5e-79
Identity = 151/309 (48.87%), Postives = 205/309 (66.34%), Query Frame = 0


Query: 4   PVIDFSKLDGEERAKTLDQIANGCEEWGFFQ---------LLERVKRISSECYKVEREES 63
           PVID SKL+GEER +T+  I + C+ WGFF+         L++ ++R++ E YK   E+ 
Sbjct: 8   PVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQK 67

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DNNEWPSNIPGFKETMREYRS 123
           FK     K L D LE     ++E++DWE  F LH     +  + P     ++  M+++  
Sbjct: 68  FKEMLRSKGL-DTLET----EVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGK 127

Query: 124 ELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRA 183
            L+ LAE+++ ++ ENLGL +GY+K  F+G  G     F TK+S+YPPCP PE++ GLRA
Sbjct: 128 RLEILAEELLDLLCENLGLEKGYLKKVFHGTTG---PTFATKLSNYPPCPKPEMIKGLRA 187

Query: 184 HTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           HTDAGG+ILLFQDD+V GLQ+LKDG W+DV PL +SIVIN GDQ+EV++NG+YKSV HRV
Sbjct: 188 HTDAGGLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRV 247

Query: 244 LATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLP 299
           +    GNR SIASFYNP  +A I+PA  LVDK     +  YP FVF DYM +YA  KF  
Sbjct: 248 MTQKEGNRMSIASFYNPGSDAEISPATSLVDK-----DSKYPSFVFDDYMKLYAGLKFQA 303

[Close]

BLAST of Lsi04G024150 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 256.1 bits (653), Expect = 3.4e-68
Identity = 140/316 (44.30%), Postives = 197/316 (62.34%), Query Frame = 0


Query: 1   MAIPVIDFSKLDGEERAKTLDQIANGCEEWGFF---------QLLERVKRISSECYKVER 60
           M IPVIDF++LDGE+R+KT+  + + C++WGFF         +L+E+VK++ +  Y+   
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-HDNNEWPSNIPGFKE----TMRE 120
           +E F  S+ VK L++        K  + DWE  F + H        IP   E    TM E
Sbjct: 69  KEKFYQSEMVKALSE-------GKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDE 128

Query: 121 YRSELKKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNG 180
           Y  +L K AE++  +M ENLGL +  I  AF+G +G     FGTKV+ YP CP PEL+ G
Sbjct: 129 YVCQLHKFAERLSKLMCENLGLDQEDIMNAFSGPKG---PAFGTKVAKYPECPRPELMRG 188

Query: 181 LRAHTDAGGVILLFQDDEVGGLQILKDGQWIDVQPLP-NSIVINTGDQIEVLSNGRYKSV 240
           LR HTDAGG+ILL QDD+V GL+  KDG+W+ + P   N+I +NTGDQ+E+LSNGRYKSV
Sbjct: 189 LREHTDAGGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSV 248

Query: 241 WHRVLATPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPK-FVFGDYMSVYAE 300
            HRV+   +G+R SIA+FYNP+ +A I+PA +L+          YP  + F DY+ +Y+ 
Sbjct: 249 VHRVMTVKHGSRLSIATFYNPAGDAIISPAPKLL----------YPSGYRFQDYLKLYST 304

The following BLAST results are available for this feature:

BLAST of Lsi04G024150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WPW4	1.4e-127	72.20	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q9FR99	1.8e-122	70.10	1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=...	[more]
P31237	1.1e-84	50.48	1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ...	[more]
Q00985	2.6e-81	49.03	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1	[more]
O48882	1.7e-80	49.35	1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 P...	[more]

BLAST of Lsi04G024150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1U8	3.0e-165	92.88	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A5A7UFF3	5.2e-165	93.20	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B9W8	5.2e-165	93.20	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103487...	[more]
A0A6J1FFA0	8.8e-165	92.88	1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschat...	[more]
A0A6J1IKI6	4.4e-164	92.56	1-aminocyclopropane-1-carboxylate oxidase 5-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of Lsi04G024150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898758.1	1.3e-165	94.82	1-aminocyclopropane-1-carboxylate oxidase 5 [Benincasa hispida]	[more]
XP_004142637.1	6.2e-165	92.88	1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis sativus] >XP_031745860.1 1-...	[more]
XP_008444197.1	1.1e-164	93.20	PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo] >KAA005220...	[more]
KAG7024737.1	1.8e-164	92.88	1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma sub...	[more]
XP_022936989.1	1.8e-164	92.88	1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 [Cucurbita moschata]...	[more]

BLAST of Lsi04G024150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77330.1	1.0e-128	72.20	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G05010.1	1.5e-79	46.98	ethylene-forming enzyme	[more]
AT1G62380.1	1.9e-79	47.15	ACC oxidase 2	[more]
AT1G12010.1	9.5e-79	48.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G19590.1	3.4e-68	44.30	ACC oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 97..124
None	No IPR available	PANTHER	PTHR47991:SF120	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE 5-LIKE	coord: 2..298
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 2..298
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..292
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 153..246 e-value: 4.0E-27 score: 94.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..300 e-value: 7.3E-87 score: 293.6
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 3..36 e-value: 2.3E-7 score: 31.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 142..246 score: 13.583211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G024150.1	Lsi04G024150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901576	organic substance biosynthetic process
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen