Lsi04G023680 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G023680 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.7e-128
Identity = 248/511 (48.53%), Postives = 338/511 (66.14%), Query Frame = 0

Query: 3   TILLYFL-FFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQY 62
           T+L  FL     ++ L +  +     N PPSP  ALP +GHLHL+   LHR+L  +S +Y
Sbjct: 5   TLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 64

Query: 63  GPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDH 122
           G +  L+ G+R VL++SSP+AA++CF++NDIVFANRP  + GK++GY++T +V +PYG+H
Sbjct: 65  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 124

Query: 123 WRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTV--VDVRTVLFEFMLNV 182
           WRNLRR++++ + S+ +L    S+R DEV  L+L L ++S Q    V++++ L E   NV
Sbjct: 125 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 184

Query: 183 MMRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKRY 242
            MRM+ GKR F  +  D++E++ F+ ++ E F+ AGA N  D+ P L+W       EK+ 
Sbjct: 185 TMRMVAGKRYFGQD-VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFK-NYEKKV 244

Query: 243 INLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIII 302
             + ++ +  +Q LI E R  K      NN       TMI+ +LSLQES P+YYTD+II 
Sbjct: 245 SKISQEMDAFLQRLIHESRINK------NN------VTMIDHLLSLQESQPEYYTDQIIK 304

Query: 303 GQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFL 362
           G +MV+L AGTDTS  T+EWAMSLLLNHP+ L KA+ EI+   G  R I E DL KL +L
Sbjct: 305 GIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYL 364

Query: 363 QCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFK 422
             +I ET R+ P  P+LVPHESS DC V GY +P+GT+L+VNAWAIH D   W++   FK
Sbjct: 365 HNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFK 424

Query: 423 PERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIG 482
           PER  G               K MPFG+GRR CPG GLA RV+GL LG+LIQCFEW+RIG
Sbjct: 425 PERHGGVELEP---------SKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIG 484

Query: 483 EEMVEMGEGTGLTMPKACPLQAKCKPRPILY 510
           E  ++M EG+GLTMPKA PL+A CKPR IL+
Sbjct: 485 EAKIDMAEGSGLTMPKAQPLEALCKPRNILH 492

BLAST of Lsi04G023680 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 9.5e-124
Identity = 241/510 (47.25%), Postives = 326/510 (63.92%), Query Frame = 0

Query: 6   LYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQY--GP 65
           L+F F F +L LL       + N PPSP    P +GHLHLLK PLHR    +S+      
Sbjct: 17  LFFFFIFLSLKLLFGPRPRKL-NLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAK 76

Query: 66  ILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDHWR 125
           I  L  GSR V ++SS + A++CF++ND+V ANRP  L GKH+GY+ T +V A YGD WR
Sbjct: 77  IFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWR 136

Query: 126 NLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQ--TVVDVRTVLFEFMLNVMM 185
           NLRRI ++ + SS  L +  S+R DE+  LI+ L+KNS      V+++ +     +N ++
Sbjct: 137 NLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNII 196

Query: 186 RMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKRYIN 245
           RM+ GKR + D T +  E+++ ++++ E     GA N  DYFP+L++       EK    
Sbjct: 197 RMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRY---VTNYEKHVKK 256

Query: 246 LRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 305
           L  + ++ +Q+L+ E R EK           +K  TMI+ +LSLQE+ PDYYTD II G 
Sbjct: 257 LAGRVDEFLQSLVNEKRVEK-----------VKGNTMIDHLLSLQETQPDYYTDVIIKGI 316

Query: 306 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQC 365
           ++VM+ AGTDTS GT+EWAMS LLNHP+VL KAK EIDD  G  R + E D+ KLP+LQ 
Sbjct: 317 ILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQH 376

Query: 366 VIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPE 425
           ++ ETLR+YPV P+L+PH +S DC V GY +PRGT+++VNAWAIH D +LWE+   FKPE
Sbjct: 377 IVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPE 436

Query: 426 RFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEE 485
           RF             G   K MPFG+GRR CPG GLA R++ L LGSL+QCFEWER+ E+
Sbjct: 437 RF----------EKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEK 496

Query: 486 MVEMGEG-TGLTMPKACPLQAKCKPRPILY 510
            ++M E   G TM KA  LQA CK RPI++
Sbjct: 497 YLDMRESEKGTTMRKATSLQAMCKARPIVH 501

BLAST of Lsi04G023680 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 6.8e-122
Identity = 238/517 (46.03%), Postives = 334/517 (64.60%), Query Frame = 0

Query: 2   VTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQY 61
           + ++LY +F    L +    L     N PPSP   LP +GHL LLKPP+HRTL   S+  
Sbjct: 6   IRVVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 65

Query: 62  -----GPILLLRFGSRPVLLISSPS-AADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVW 121
                G ++ LR GSR V ++SS   AA++CF +ND+V ANRP+++ GKH+GY+ T ++ 
Sbjct: 66  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 125

Query: 122 APYGDHWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSK--NSNQTVVDVRTVLF 181
           APYGDHWRNLRR+ ++ + S+  L     VR DEV  LI RLS+   + +TVV+++ +L 
Sbjct: 126 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 185

Query: 182 EFMLNVMMRMIDGKRCFNDNTTDTEESRNFQEIVTET-FKLAGANLVDYFPMLKWTGITW 241
           +   N +MRM+ GKR + + TTD EE++  +++V +     +  N VDY P+L+   +  
Sbjct: 186 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILR---LFS 245

Query: 242 KIEKRYINLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYY 301
             E R   L ++ +K +Q LI++ R ++E             TTMI+ +L LQ+SD +YY
Sbjct: 246 SYENRVKKLGEETDKFLQGLIDDKRGQQE-----------TGTTMIDHLLVLQKSDIEYY 305

Query: 302 TDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADL 361
           TD+II G +++M+ AGT+TS  T+EWA+S LLNHPDV++KA+ EID+  G  R I EADL
Sbjct: 306 TDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADL 365

Query: 362 EKLPFLQCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWE 421
            +LP+L+ ++ ETLR++P  PLLVPH +S DC +  Y +PRGT L+VNAWAIH D   W+
Sbjct: 366 SELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWD 425

Query: 422 DAAAFKPERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCF 481
           D  +FKPERF     A           K + FG+GRR CPG GLA R++GL LGSLIQCF
Sbjct: 426 DPDSFKPERFEKEEEAQ----------KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCF 485

Query: 482 EWERIGEEMVEMGEGTGLTMPKACPLQAKCKPRPILY 510
           EWER+G   V+M EG G T+PKA PL+A CK RP L+
Sbjct: 486 EWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLH 498

BLAST of Lsi04G023680 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 7.8e-118
Identity = 236/510 (46.27%), Postives = 320/510 (62.75%), Query Frame = 0

Query: 6   LYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYGPIL 65
           L  L F   + +L+      + N PP P P +P +G+LH LK PLHRT   +S  YG I 
Sbjct: 7   LLSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYGDIF 66

Query: 66  LLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDHWRNL 125
            L FGSR V+++SSPS A +CF++NDI+ ANRPR L GK++ Y++T +  A YGDHWRNL
Sbjct: 67  SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 126

Query: 126 RRISSLHLLSSANLQALSSVRADEVHSLILRL-----SKNSNQTVVDVRTVLFEFMLNVM 185
           RRI+++ +LS+  L +   VR DE + LI +L     S+    T V++R  L E   N M
Sbjct: 127 RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 186

Query: 186 MRMIDGKRCFND--NTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKR 245
           MRMI GKR + D  + +D EE++ F+EI++E   L GA N  D+ P+L+   +   +EKR
Sbjct: 187 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD-NLEKR 246

Query: 246 YINLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEII 305
              + K+ N  ++ LIEEHRR        N  S     TMI+ +L L ES P+YY+D +I
Sbjct: 247 CKRIAKRSNAFLEGLIEEHRR-------GNIHS--DGGTMIDHLLKLSESQPEYYSDHLI 306

Query: 306 IGQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPF 365
            G +  ML AGTDTS  T+EW MS LLNHP+VL KAK E+D   G+ + + E DL KLP+
Sbjct: 307 KGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPY 366

Query: 366 LQCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAF 425
           LQ +I ETLR++P  PLL+PH SS DCT+  +++P+ T+++ N W IH D + W DA +F
Sbjct: 367 LQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSF 426

Query: 426 KPERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERI 485
           KPERF         E  + +  K M FG+GRR CPG  LA R +G  +G LIQCFEWER 
Sbjct: 427 KPERF---------EKEEEVN-KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERE 486

Query: 486 GEEMVEMGEGTGLTMPKACPLQAKCKPRPI 508
            EE ++M EG G+TMP   PL+A CK  PI
Sbjct: 487 SEEKLDMMEGKGITMPMKIPLRAMCKALPI 495

BLAST of Lsi04G023680 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.7e-117
Identity = 230/508 (45.28%), Postives = 319/508 (62.80%), Query Frame = 0

Query: 7   YFLFFFYALHLLIHHLL----TNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYG 66
           Y +    AL LL + L     T   N PPSP  +LP LGH +LLKPP+HR   ++S  +G
Sbjct: 4   YVIVLPLALFLLAYKLFFTSKTKRFNLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHG 63

Query: 67  PILLLRFGSRPVLLISSPSAADQCFS-QNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDH 126
           PI  L+FGSR  ++ISS S A QCF+ QNDI+ +NRP  L  K++ Y++T V  APYGDH
Sbjct: 64  PIFSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDH 123

Query: 127 WRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVMM 186
           WRNLRRI SL +LSS  L     +R DE+H ++ RLS++ N+  +++  +L +   N ++
Sbjct: 124 WRNLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKE-IELEPLLSDLTFNNIV 183

Query: 187 RMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKRYIN 246
           RM+ GKR + D   + EE+  F+++V +    +GA +  DY P +K  G     EK+   
Sbjct: 184 RMVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFG--GSFEKKVKA 243

Query: 247 LRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 306
           L +  ++++Q L+EE +R+K+              TM+  +LSLQ+++P+YYTD  I G 
Sbjct: 244 LAEAMDEILQRLLEECKRDKD------------GNTMVNHLLSLQQNEPEYYTDVTIKGL 303

Query: 307 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQC 366
           M+ M+ AGTDTS  T+EWAMS LLNHP+ L KAK EID+  G++R I E D+  LP+LQ 
Sbjct: 304 MLGMMIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDIANLPYLQN 363

Query: 367 VIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPE 426
           ++ ET R+YP  PLLVP   + D  V GY +PRGTM++VNAWAIH D  LW +   FKPE
Sbjct: 364 IVSETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKFKPE 423

Query: 427 RFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEE 486
           RF G      GE       K MPFG GRR CPG GL  +++ L LGSLIQCF+W+++  E
Sbjct: 424 RFNGGEGGGRGEDVH----KLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGE 483

Query: 487 MVEMGEGTGLTMPKACPLQAKCKPRPIL 509
            ++M E  G+ M K  PL A C+ RPI+
Sbjct: 484 AIDMTETPGMAMRKKIPLSALCQSRPIM 492

BLAST of Lsi04G023680 vs. ExPASy TrEMBL
Match: A0A5D3D6T6 (Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00050 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.4e-247
Identity = 436/512 (85.16%), Postives = 471/512 (91.99%), Query Frame = 0

Query: 1   MVTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQ 60
           MVT LL+FLFFFY LHL+IHHLL  I NRPPSPFPALPFLGHLHLLKPPLHRTLAKIS +
Sbjct: 1   MVTTLLFFLFFFYTLHLVIHHLLCKIQNRPPSPFPALPFLGHLHLLKPPLHRTLAKISHK 60

Query: 61  YGPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 120
           YGPILLLRFGSRPVLLISSPSAA QCFSQNDIVFANRPRLLAGKHLGYDF+AVVWA YGD
Sbjct: 61  YGPILLLRFGSRPVLLISSPSAALQCFSQNDIVFANRPRLLAGKHLGYDFSAVVWASYGD 120

Query: 121 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVM 180
           HWRNLRR+ SLHLLSSANLQ+LSSVRADEVHSLILRL KNSNQ VV+VRT+LFEFMLNVM
Sbjct: 121 HWRNLRRLLSLHLLSSANLQSLSSVRADEVHSLILRLCKNSNQ-VVNVRTLLFEFMLNVM 180

Query: 181 MRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYIN 240
           MRMI GKR F+DNTT +EES NFQEI+ ETFKLA ANL+DY P+LKWTGI+ KIEKRYIN
Sbjct: 181 MRMIGGKRYFDDNTTPSEESLNFQEIIAETFKLATANLMDYLPILKWTGISRKIEKRYIN 240

Query: 241 LRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 300
           LRKKR+KL+QNLIEEHR+EK++ M +NNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ
Sbjct: 241 LRKKRDKLVQNLIEEHRKEKQQAM-SNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 300

Query: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQC 360
           MMVMLSAGTDTSVGTMEWAMSLLLNHPDVL KA+ EID+I G+KRHI E+DLEKLP+LQC
Sbjct: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLGKAQEEIDEIVGKKRHIEESDLEKLPYLQC 360

Query: 361 VIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPE 420
           VIKET+RMYP GPLLVPHESSADCTV GYHIPRGTML+VNAWAIHNDA LWE+AA FKPE
Sbjct: 361 VIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGLWEEAAVFKPE 420

Query: 421 RFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEE 480
           RFL A+AA     GDG GLKYM FG GRRGCPGEGLA++V+GLVLGSLIQCFEWERIGEE
Sbjct: 421 RFLAAAAAE----GDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEWERIGEE 480

Query: 481 MVEMGEGTGLTMPKACPLQAKCKPRPILYCTM 513
           MV+MGEGTGL+MPKACPLQAKC PRPIL+  M
Sbjct: 481 MVDMGEGTGLSMPKACPLQAKCSPRPILHYVM 506

BLAST of Lsi04G023680 vs. ExPASy TrEMBL
Match: A0A0A0KY88 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G376150 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.4e-247
Identity = 441/513 (85.96%), Postives = 470/513 (91.62%), Query Frame = 0

Query: 1   MVTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQ 60
           MVTILLYFLFFFY LHLLIHHLL  I NRPPSPFP LPFLGHLHLLKPPLHR+LAKIS +
Sbjct: 1   MVTILLYFLFFFYTLHLLIHHLLCKIQNRPPSPFPTLPFLGHLHLLKPPLHRSLAKISHK 60

Query: 61  YGPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 120
           YGPILLLRFGSRPVLLISSPSAA QCFSQNDIVFANRPRLLAGK+LGYDF+ VVWA YGD
Sbjct: 61  YGPILLLRFGSRPVLLISSPSAALQCFSQNDIVFANRPRLLAGKYLGYDFSVVVWASYGD 120

Query: 121 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVM 180
           HWRNLRRISSLHLLSS+NLQ+LSSVRADEVHSLILRL KN NQ VV+VRT+LFEFMLNVM
Sbjct: 121 HWRNLRRISSLHLLSSSNLQSLSSVRADEVHSLILRLRKNPNQ-VVNVRTLLFEFMLNVM 180

Query: 181 MRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKRYI 240
           MRMI GKR F+DN T TEES NFQEIVTETFKLAGA NLVDY P+LKWTGI+ KIEKRYI
Sbjct: 181 MRMIGGKRYFDDNRTHTEESLNFQEIVTETFKLAGANNLVDYLPILKWTGISRKIEKRYI 240

Query: 241 NLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIG 300
           NLRKKR+KLIQNLIEEHR+EK+K M + NK PLKKTTMIE+MLSLQESDPDYYTDEIIIG
Sbjct: 241 NLRKKRDKLIQNLIEEHRKEKQKAM-SKNKPPLKKTTMIEMMLSLQESDPDYYTDEIIIG 300

Query: 301 QMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQ 360
           QMMVMLSAGTDTSVGTMEWAMSLLLNHPDVL KAK E+D+I G+KRHI E+DLEKLP+LQ
Sbjct: 301 QMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLGKAKEEMDEIIGKKRHIEESDLEKLPYLQ 360

Query: 361 CVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKP 420
           CVIKET+RMYPVGPLLVPHESSADCTV GYHIP GTML+VNAWAIHNDA LWE+AA FKP
Sbjct: 361 CVIKETMRMYPVGPLLVPHESSADCTVGGYHIPGGTMLMVNAWAIHNDAGLWEEAAVFKP 420

Query: 421 ERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGE 480
           ERFLGA     G  GDGIGLKYM FG GRRGCPGEGLA+RV+GLVLGSLIQCFEWERIGE
Sbjct: 421 ERFLGA-----GAEGDGIGLKYMVFGAGRRGCPGEGLAMRVVGLVLGSLIQCFEWERIGE 480

Query: 481 EMVEMGEGTGLTMPKACPLQAKCKPRPILYCTM 513
           EMV+MGEGTGLTMPKACPLQAKC+PRPIL+  M
Sbjct: 481 EMVDMGEGTGLTMPKACPLQAKCRPRPILHYVM 506

BLAST of Lsi04G023680 vs. ExPASy TrEMBL
Match: A0A1S3B958 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103487563 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.4e-247
Identity = 436/512 (85.16%), Postives = 471/512 (91.99%), Query Frame = 0

Query: 1   MVTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQ 60
           MVT LL+FLFFFY LHL+IHHLL  I NRPPSPFPALPFLGHLHLLKPPLHRTLAKIS +
Sbjct: 1   MVTTLLFFLFFFYTLHLVIHHLLCKIQNRPPSPFPALPFLGHLHLLKPPLHRTLAKISHK 60

Query: 61  YGPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 120
           YGPILLLRFGSRPVLLISSPSAA QCFSQNDIVFANRPRLLAGKHLGYDF+AVVWA YGD
Sbjct: 61  YGPILLLRFGSRPVLLISSPSAALQCFSQNDIVFANRPRLLAGKHLGYDFSAVVWASYGD 120

Query: 121 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVM 180
           HWRNLRR+ SLHLLSSANLQ+LSSVRADEVHSLILRL KNSNQ VV+VRT+LFEFMLNVM
Sbjct: 121 HWRNLRRLLSLHLLSSANLQSLSSVRADEVHSLILRLCKNSNQ-VVNVRTLLFEFMLNVM 180

Query: 181 MRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYIN 240
           MRMI GKR F+DNTT +EES NFQEI+ ETFKLA ANL+DY P+LKWTGI+ KIEKRYIN
Sbjct: 181 MRMIGGKRYFDDNTTPSEESLNFQEIIAETFKLATANLMDYLPILKWTGISRKIEKRYIN 240

Query: 241 LRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 300
           LRKKR+KL+QNLIEEHR+EK++ M +NNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ
Sbjct: 241 LRKKRDKLVQNLIEEHRKEKQQAM-SNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 300

Query: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQC 360
           MMVMLSAGTDTSVGTMEWAMSLLLNHPDVL KA+ EID+I G+KRHI E+DLEKLP+LQC
Sbjct: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLGKAQEEIDEIVGKKRHIEESDLEKLPYLQC 360

Query: 361 VIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPE 420
           VIKET+RMYP GPLLVPHESSADCTV GYHIPRGTML+VNAWAIHNDA LWE+AA FKPE
Sbjct: 361 VIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGLWEEAAVFKPE 420

Query: 421 RFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEE 480
           RFL A+AA     GDG GLKYM FG GRRGCPGEGLA++V+GLVLGSLIQCFEWERIGEE
Sbjct: 421 RFLAAAAAE----GDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEWERIGEE 480

Query: 481 MVEMGEGTGLTMPKACPLQAKCKPRPILYCTM 513
           MV+MGEGTGL+MPKACPLQAKC PRPIL+  M
Sbjct: 481 MVDMGEGTGLSMPKACPLQAKCSPRPILHYVM 506

BLAST of Lsi04G023680 vs. ExPASy TrEMBL
Match: A0A5A7UDX9 (Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1994G00040 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.7e-238
Identity = 417/488 (85.45%), Postives = 451/488 (92.42%), Query Frame = 0

Query: 25  NIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYGPILLLRFGSRPVLLISSPSAAD 84
           +I NRPPSPFPALPFLGHLHLLKPPLHRTLAKIS +YGPILLLRFGSRPVLLISSPSAA 
Sbjct: 61  DIQNRPPSPFPALPFLGHLHLLKPPLHRTLAKISHKYGPILLLRFGSRPVLLISSPSAAL 120

Query: 85  QCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDHWRNLRRISSLHLLSSANLQALSS 144
           QCFSQNDIVFANRPRLLAGKHLGYDF+AVVWA YGDHWRNLRR+ SLHLLSSANLQ+LSS
Sbjct: 121 QCFSQNDIVFANRPRLLAGKHLGYDFSAVVWASYGDHWRNLRRLLSLHLLSSANLQSLSS 180

Query: 145 VRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVMMRMIDGKRCFNDNTTDTEESRNFQ 204
           VRADEVHSLILRL KNSNQ VV+VRT+LFEFMLNVMMRMI GKR F+DNTT +EES NFQ
Sbjct: 181 VRADEVHSLILRLCKNSNQ-VVNVRTLLFEFMLNVMMRMIGGKRYFDDNTTPSEESLNFQ 240

Query: 205 EIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYINLRKKRNKLIQNLIEEHRREKEKMM 264
           EI+ ETFKLA ANL+DY P+LKWTGI+ KIEKRYINLRKKR+KL+QNLIEEHR+EK++ M
Sbjct: 241 EIIAETFKLATANLMDYLPILKWTGISRKIEKRYINLRKKRDKLVQNLIEEHRKEKQQAM 300

Query: 265 TNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLL 324
            +NNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLL
Sbjct: 301 -SNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLL 360

Query: 325 NHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQCVIKETLRMYPVGPLLVPHESSADC 384
           NHPDVL KA+ EID+I G+KRHI E+DLEKLP+LQCVIKET+RMYP GPLLVPHESSADC
Sbjct: 361 NHPDVLGKAQEEIDEIVGKKRHIEESDLEKLPYLQCVIKETMRMYPAGPLLVPHESSADC 420

Query: 385 TVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPERFLGASAAAVGEVGDGIGLKYMPF 444
           TV GYHIPRGTML+VNAWAIHNDA LWE+AA FKPERFL A+AA     GDG GLKYM F
Sbjct: 421 TVGGYHIPRGTMLMVNAWAIHNDAGLWEEAAVFKPERFLAAAAAE----GDGTGLKYMVF 480

Query: 445 GVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEEMVEMGEGTGLTMPKACPLQAKCKP 504
           G GRRGCPGEGLA++V+GLVLGSLIQCFEWERIGEEMV+MGEGTGL+MPKACPLQAKC P
Sbjct: 481 GAGRRGCPGEGLAMKVVGLVLGSLIQCFEWERIGEEMVDMGEGTGLSMPKACPLQAKCSP 540

Query: 505 RPILYCTM 513
           RPIL+  M
Sbjct: 541 RPILHYVM 542

BLAST of Lsi04G023680 vs. ExPASy TrEMBL
Match: A0A6J1CEJ7 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111010788 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.6e-217
Identity = 384/511 (75.15%), Postives = 440/511 (86.11%), Query Frame = 0

Query: 4   ILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYGP 63
           ILLYF FFFYALH+LIH LL+ +  RPPSPFPALPF+GHL LLKPPLHRTLAKISD+YGP
Sbjct: 4   ILLYF-FFFYALHVLIHRLLSRVQKRPPSPFPALPFVGHLQLLKPPLHRTLAKISDRYGP 63

Query: 64  ILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDHWR 123
           +LLLRFGSRPVLL+SSP AAD+CF+ ND+ FANRPRLLAGKHLGYD+TA+VWAPYGDHWR
Sbjct: 64  VLLLRFGSRPVLLVSSPFAADECFTINDVTFANRPRLLAGKHLGYDYTALVWAPYGDHWR 123

Query: 124 NLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVMMRM 183
           NLRRI+S+HLLSS NLQALSS RADEVHSL+  L + S+Q +VDVRTVLFEFMLNVMM M
Sbjct: 124 NLRRIASVHLLSSTNLQALSSTRADEVHSLLHSLHQKSDQ-IVDVRTVLFEFMLNVMMTM 183

Query: 184 IDGKRCFNDNTTDTEESRNFQEIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYINLRK 243
           I GKR F DNT D EESRNFQEIV+ETF+L+G N  DY P+LKW   + +IE+ YINLRK
Sbjct: 184 IGGKRYFGDNTADAEESRNFQEIVSETFRLSGTNFADYLPILKWIIGSRRIERSYINLRK 243

Query: 244 KRNKLIQNLIEEHRREKEKMMTNNNKSPL-----KKTTMIEVMLSLQESDPDYYTDEIII 303
           +R+  +QNLIEEHR  K+  M    +SPL     KK TMIEVMLSLQESDPDYYTDEIII
Sbjct: 244 RRDGFMQNLIEEHREIKKLEM--RTQSPLSGETKKKKTMIEVMLSLQESDPDYYTDEIII 303

Query: 304 GQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFL 363
           G M+V+L+AGTDT+VGTMEWAMSLLLNHP+VLA  +AEIDD+ G++RH+ E+DLEKLP+L
Sbjct: 304 GLMLVLLTAGTDTTVGTMEWAMSLLLNHPNVLANVRAEIDDVVGQERHVDESDLEKLPYL 363

Query: 364 QCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFK 423
           QCVIKETLRM+PVGPLLVPHESSADCTV GYHIPRGTML+VNAWAIHND   WE+ AAF+
Sbjct: 364 QCVIKETLRMHPVGPLLVPHESSADCTVGGYHIPRGTMLLVNAWAIHNDGGNWEEPAAFR 423

Query: 424 PERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIG 483
           PERFL  +A      G G GL+Y+PFG GRRGCPGE LA++V+GLV+GSLIQCFEW+RIG
Sbjct: 424 PERFLEVAA------GAGDGLRYVPFGFGRRGCPGETLAVKVVGLVVGSLIQCFEWDRIG 483

Query: 484 EEMVEMGEGTGLTMPKACPLQAKCKPRPILY 510
           EEMV+M EGTGLTMPKA PLQAKC+PRPILY
Sbjct: 484 EEMVDMAEGTGLTMPKAHPLQAKCRPRPILY 504

BLAST of Lsi04G023680 vs. NCBI nr
Match: XP_038897481.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 874.0 bits (2257), Expect = 6.3e-250
Identity = 437/512 (85.35%), Postives = 470/512 (91.80%), Query Frame = 0

Query: 1   MVTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQ 60
           MVTILLYFL  FYALHLLIH LL   HNRPPSPFPALPFLGH HLLKPPLHRTLAKISDQ
Sbjct: 1   MVTILLYFL-CFYALHLLIHRLLAKFHNRPPSPFPALPFLGHFHLLKPPLHRTLAKISDQ 60

Query: 61  YGPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 120
           YG +LLLRFGSRPVLL+SSPSAA +CFSQNDIVFANRPR LA KHLGYDFT VVWA YGD
Sbjct: 61  YGSVLLLRFGSRPVLLVSSPSAAHECFSQNDIVFANRPRFLASKHLGYDFTTVVWASYGD 120

Query: 121 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVM 180
            WR+LRRISSLHLLSSANLQALSSVR+DEVHSLILRL K SNQ++V+VRTVLFEFMLNVM
Sbjct: 121 RWRDLRRISSLHLLSSANLQALSSVRSDEVHSLILRLCKTSNQSIVNVRTVLFEFMLNVM 180

Query: 181 MRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYIN 240
           MRMI GKR F DNTT TEESR FQEIVTETFKL+G NLVDYFP+LKWTGI+ K EKRYIN
Sbjct: 181 MRMIGGKRYFGDNTTHTEESRKFQEIVTETFKLSGVNLVDYFPILKWTGISRKFEKRYIN 240

Query: 241 LRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 300
           LRKKR++LIQN+IEEHR+EKEKMM ++NKSPLKKTTMIEVMLSLQESDP+YYTDE++IGQ
Sbjct: 241 LRKKRDELIQNIIEEHRKEKEKMMMSSNKSPLKKTTMIEVMLSLQESDPEYYTDEVVIGQ 300

Query: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQC 360
           MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDI GEKRH+ E+DLEKLP+LQ 
Sbjct: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIVGEKRHMEESDLEKLPYLQA 360

Query: 361 VIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPE 420
           VIKET RMYPVGPLLVPHESSADC V GYH+PRGTMLVVNAWAIHNDAR WE+AAAFKPE
Sbjct: 361 VIKETQRMYPVGPLLVPHESSADCFVGGYHVPRGTMLVVNAWAIHNDARNWEEAAAFKPE 420

Query: 421 RFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEE 480
           RF+GA+A    E GDGIGLKYM FGVGRRGCPGEGLA+RV+GL LGSLIQCFEWERIGEE
Sbjct: 421 RFMGAAAV---EEGDGIGLKYMAFGVGRRGCPGEGLAMRVVGLALGSLIQCFEWERIGEE 480

Query: 481 MVEMGEGTGLTMPKACPLQAKCKPRPILYCTM 513
           MV+M EGTGLTMPKACPL+AKC+PRPIL+  M
Sbjct: 481 MVDMSEGTGLTMPKACPLRAKCRPRPILHYAM 508

BLAST of Lsi04G023680 vs. NCBI nr
Match: XP_008444122.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo] >TYK19213.1 cytochrome P450 81D1-like [Cucumis melo var. makuwa])

HSP 1 Score: 865.1 bits (2234), Expect = 2.9e-247
Identity = 436/512 (85.16%), Postives = 471/512 (91.99%), Query Frame = 0

Query: 1   MVTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQ 60
           MVT LL+FLFFFY LHL+IHHLL  I NRPPSPFPALPFLGHLHLLKPPLHRTLAKIS +
Sbjct: 1   MVTTLLFFLFFFYTLHLVIHHLLCKIQNRPPSPFPALPFLGHLHLLKPPLHRTLAKISHK 60

Query: 61  YGPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 120
           YGPILLLRFGSRPVLLISSPSAA QCFSQNDIVFANRPRLLAGKHLGYDF+AVVWA YGD
Sbjct: 61  YGPILLLRFGSRPVLLISSPSAALQCFSQNDIVFANRPRLLAGKHLGYDFSAVVWASYGD 120

Query: 121 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVM 180
           HWRNLRR+ SLHLLSSANLQ+LSSVRADEVHSLILRL KNSNQ VV+VRT+LFEFMLNVM
Sbjct: 121 HWRNLRRLLSLHLLSSANLQSLSSVRADEVHSLILRLCKNSNQ-VVNVRTLLFEFMLNVM 180

Query: 181 MRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYIN 240
           MRMI GKR F+DNTT +EES NFQEI+ ETFKLA ANL+DY P+LKWTGI+ KIEKRYIN
Sbjct: 181 MRMIGGKRYFDDNTTPSEESLNFQEIIAETFKLATANLMDYLPILKWTGISRKIEKRYIN 240

Query: 241 LRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 300
           LRKKR+KL+QNLIEEHR+EK++ M +NNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ
Sbjct: 241 LRKKRDKLVQNLIEEHRKEKQQAM-SNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 300

Query: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQC 360
           MMVMLSAGTDTSVGTMEWAMSLLLNHPDVL KA+ EID+I G+KRHI E+DLEKLP+LQC
Sbjct: 301 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLGKAQEEIDEIVGKKRHIEESDLEKLPYLQC 360

Query: 361 VIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPE 420
           VIKET+RMYP GPLLVPHESSADCTV GYHIPRGTML+VNAWAIHNDA LWE+AA FKPE
Sbjct: 361 VIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGLWEEAAVFKPE 420

Query: 421 RFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEE 480
           RFL A+AA     GDG GLKYM FG GRRGCPGEGLA++V+GLVLGSLIQCFEWERIGEE
Sbjct: 421 RFLAAAAAE----GDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEWERIGEE 480

Query: 481 MVEMGEGTGLTMPKACPLQAKCKPRPILYCTM 513
           MV+MGEGTGL+MPKACPLQAKC PRPIL+  M
Sbjct: 481 MVDMGEGTGLSMPKACPLQAKCSPRPILHYVM 506

BLAST of Lsi04G023680 vs. NCBI nr
Match: XP_004142688.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN54600.1 hypothetical protein Csa_012553 [Cucumis sativus])

HSP 1 Score: 865.1 bits (2234), Expect = 2.9e-247
Identity = 441/513 (85.96%), Postives = 470/513 (91.62%), Query Frame = 0

Query: 1   MVTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQ 60
           MVTILLYFLFFFY LHLLIHHLL  I NRPPSPFP LPFLGHLHLLKPPLHR+LAKIS +
Sbjct: 1   MVTILLYFLFFFYTLHLLIHHLLCKIQNRPPSPFPTLPFLGHLHLLKPPLHRSLAKISHK 60

Query: 61  YGPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 120
           YGPILLLRFGSRPVLLISSPSAA QCFSQNDIVFANRPRLLAGK+LGYDF+ VVWA YGD
Sbjct: 61  YGPILLLRFGSRPVLLISSPSAALQCFSQNDIVFANRPRLLAGKYLGYDFSVVVWASYGD 120

Query: 121 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVM 180
           HWRNLRRISSLHLLSS+NLQ+LSSVRADEVHSLILRL KN NQ VV+VRT+LFEFMLNVM
Sbjct: 121 HWRNLRRISSLHLLSSSNLQSLSSVRADEVHSLILRLRKNPNQ-VVNVRTLLFEFMLNVM 180

Query: 181 MRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKRYI 240
           MRMI GKR F+DN T TEES NFQEIVTETFKLAGA NLVDY P+LKWTGI+ KIEKRYI
Sbjct: 181 MRMIGGKRYFDDNRTHTEESLNFQEIVTETFKLAGANNLVDYLPILKWTGISRKIEKRYI 240

Query: 241 NLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIG 300
           NLRKKR+KLIQNLIEEHR+EK+K M + NK PLKKTTMIE+MLSLQESDPDYYTDEIIIG
Sbjct: 241 NLRKKRDKLIQNLIEEHRKEKQKAM-SKNKPPLKKTTMIEMMLSLQESDPDYYTDEIIIG 300

Query: 301 QMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQ 360
           QMMVMLSAGTDTSVGTMEWAMSLLLNHPDVL KAK E+D+I G+KRHI E+DLEKLP+LQ
Sbjct: 301 QMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLGKAKEEMDEIIGKKRHIEESDLEKLPYLQ 360

Query: 361 CVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKP 420
           CVIKET+RMYPVGPLLVPHESSADCTV GYHIP GTML+VNAWAIHNDA LWE+AA FKP
Sbjct: 361 CVIKETMRMYPVGPLLVPHESSADCTVGGYHIPGGTMLMVNAWAIHNDAGLWEEAAVFKP 420

Query: 421 ERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGE 480
           ERFLGA     G  GDGIGLKYM FG GRRGCPGEGLA+RV+GLVLGSLIQCFEWERIGE
Sbjct: 421 ERFLGA-----GAEGDGIGLKYMVFGAGRRGCPGEGLAMRVVGLVLGSLIQCFEWERIGE 480

Query: 481 EMVEMGEGTGLTMPKACPLQAKCKPRPILYCTM 513
           EMV+MGEGTGLTMPKACPLQAKC+PRPIL+  M
Sbjct: 481 EMVDMGEGTGLTMPKACPLQAKCRPRPILHYVM 506

BLAST of Lsi04G023680 vs. NCBI nr
Match: KAA0052386.1 (cytochrome P450 81D1-like [Cucumis melo var. makuwa])

HSP 1 Score: 834.3 bits (2154), Expect = 5.5e-238
Identity = 417/488 (85.45%), Postives = 451/488 (92.42%), Query Frame = 0

Query: 25  NIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYGPILLLRFGSRPVLLISSPSAAD 84
           +I NRPPSPFPALPFLGHLHLLKPPLHRTLAKIS +YGPILLLRFGSRPVLLISSPSAA 
Sbjct: 61  DIQNRPPSPFPALPFLGHLHLLKPPLHRTLAKISHKYGPILLLRFGSRPVLLISSPSAAL 120

Query: 85  QCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDHWRNLRRISSLHLLSSANLQALSS 144
           QCFSQNDIVFANRPRLLAGKHLGYDF+AVVWA YGDHWRNLRR+ SLHLLSSANLQ+LSS
Sbjct: 121 QCFSQNDIVFANRPRLLAGKHLGYDFSAVVWASYGDHWRNLRRLLSLHLLSSANLQSLSS 180

Query: 145 VRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVMMRMIDGKRCFNDNTTDTEESRNFQ 204
           VRADEVHSLILRL KNSNQ VV+VRT+LFEFMLNVMMRMI GKR F+DNTT +EES NFQ
Sbjct: 181 VRADEVHSLILRLCKNSNQ-VVNVRTLLFEFMLNVMMRMIGGKRYFDDNTTPSEESLNFQ 240

Query: 205 EIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYINLRKKRNKLIQNLIEEHRREKEKMM 264
           EI+ ETFKLA ANL+DY P+LKWTGI+ KIEKRYINLRKKR+KL+QNLIEEHR+EK++ M
Sbjct: 241 EIIAETFKLATANLMDYLPILKWTGISRKIEKRYINLRKKRDKLVQNLIEEHRKEKQQAM 300

Query: 265 TNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLL 324
            +NNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLL
Sbjct: 301 -SNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLL 360

Query: 325 NHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQCVIKETLRMYPVGPLLVPHESSADC 384
           NHPDVL KA+ EID+I G+KRHI E+DLEKLP+LQCVIKET+RMYP GPLLVPHESSADC
Sbjct: 361 NHPDVLGKAQEEIDEIVGKKRHIEESDLEKLPYLQCVIKETMRMYPAGPLLVPHESSADC 420

Query: 385 TVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPERFLGASAAAVGEVGDGIGLKYMPF 444
           TV GYHIPRGTML+VNAWAIHNDA LWE+AA FKPERFL A+AA     GDG GLKYM F
Sbjct: 421 TVGGYHIPRGTMLMVNAWAIHNDAGLWEEAAVFKPERFLAAAAAE----GDGTGLKYMVF 480

Query: 445 GVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEEMVEMGEGTGLTMPKACPLQAKCKP 504
           G GRRGCPGEGLA++V+GLVLGSLIQCFEWERIGEEMV+MGEGTGL+MPKACPLQAKC P
Sbjct: 481 GAGRRGCPGEGLAMKVVGLVLGSLIQCFEWERIGEEMVDMGEGTGLSMPKACPLQAKCSP 540

Query: 505 RPILYCTM 513
           RPIL+  M
Sbjct: 541 RPILHYVM 542

BLAST of Lsi04G023680 vs. NCBI nr
Match: XP_022140041.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 764.6 bits (1973), Expect = 5.4e-217
Identity = 384/511 (75.15%), Postives = 440/511 (86.11%), Query Frame = 0

Query: 4   ILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYGP 63
           ILLYF FFFYALH+LIH LL+ +  RPPSPFPALPF+GHL LLKPPLHRTLAKISD+YGP
Sbjct: 4   ILLYF-FFFYALHVLIHRLLSRVQKRPPSPFPALPFVGHLQLLKPPLHRTLAKISDRYGP 63

Query: 64  ILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDHWR 123
           +LLLRFGSRPVLL+SSP AAD+CF+ ND+ FANRPRLLAGKHLGYD+TA+VWAPYGDHWR
Sbjct: 64  VLLLRFGSRPVLLVSSPFAADECFTINDVTFANRPRLLAGKHLGYDYTALVWAPYGDHWR 123

Query: 124 NLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQTVVDVRTVLFEFMLNVMMRM 183
           NLRRI+S+HLLSS NLQALSS RADEVHSL+  L + S+Q +VDVRTVLFEFMLNVMM M
Sbjct: 124 NLRRIASVHLLSSTNLQALSSTRADEVHSLLHSLHQKSDQ-IVDVRTVLFEFMLNVMMTM 183

Query: 184 IDGKRCFNDNTTDTEESRNFQEIVTETFKLAGANLVDYFPMLKWTGITWKIEKRYINLRK 243
           I GKR F DNT D EESRNFQEIV+ETF+L+G N  DY P+LKW   + +IE+ YINLRK
Sbjct: 184 IGGKRYFGDNTADAEESRNFQEIVSETFRLSGTNFADYLPILKWIIGSRRIERSYINLRK 243

Query: 244 KRNKLIQNLIEEHRREKEKMMTNNNKSPL-----KKTTMIEVMLSLQESDPDYYTDEIII 303
           +R+  +QNLIEEHR  K+  M    +SPL     KK TMIEVMLSLQESDPDYYTDEIII
Sbjct: 244 RRDGFMQNLIEEHREIKKLEM--RTQSPLSGETKKKKTMIEVMLSLQESDPDYYTDEIII 303

Query: 304 GQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFL 363
           G M+V+L+AGTDT+VGTMEWAMSLLLNHP+VLA  +AEIDD+ G++RH+ E+DLEKLP+L
Sbjct: 304 GLMLVLLTAGTDTTVGTMEWAMSLLLNHPNVLANVRAEIDDVVGQERHVDESDLEKLPYL 363

Query: 364 QCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFK 423
           QCVIKETLRM+PVGPLLVPHESSADCTV GYHIPRGTML+VNAWAIHND   WE+ AAF+
Sbjct: 364 QCVIKETLRMHPVGPLLVPHESSADCTVGGYHIPRGTMLLVNAWAIHNDGGNWEEPAAFR 423

Query: 424 PERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIG 483
           PERFL  +A      G G GL+Y+PFG GRRGCPGE LA++V+GLV+GSLIQCFEW+RIG
Sbjct: 424 PERFLEVAA------GAGDGLRYVPFGFGRRGCPGETLAVKVVGLVVGSLIQCFEWDRIG 483

Query: 484 EEMVEMGEGTGLTMPKACPLQAKCKPRPILY 510
           EEMV+M EGTGLTMPKA PLQAKC+PRPILY
Sbjct: 484 EEMVDMAEGTGLTMPKAHPLQAKCRPRPILY 504

BLAST of Lsi04G023680 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 449.1 bits (1154), Expect = 4.7e-126
Identity = 242/513 (47.17%), Postives = 330/513 (64.33%), Query Frame = 0

Query: 3   TILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYG 62
           T++  FLF   +L  +I  +     N PPSP  ALP +GHL LLKPPLHR    +S+  G
Sbjct: 5   TLIFTFLFISLSLTFIIGRIKRR-PNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLG 64

Query: 63  --PILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 122
             PI+ LR G+R V ++SS S A++CF++ND+V ANR   LA KH+ Y  T VV A YGD
Sbjct: 65  DAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGD 124

Query: 123 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQ--TVVDVRTVLFEFMLN 182
           HWRNLRRI ++ + S+  L + SS+R DE+H LI  LS+NS+   T V+++++      N
Sbjct: 125 HWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFN 184

Query: 183 VMMRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKW-TGITWKIEK 242
            ++RM+ GK  + D   D  E++  +E++ E     GA N  DY P+L W TG     EK
Sbjct: 185 NIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWITG----SEK 244

Query: 243 RYINLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEI 302
           R   +  + ++ +Q L++E R  KEK          ++ TM++ +L LQE+ P+YYTD I
Sbjct: 245 RIKKIASRLDEFLQGLVDERREGKEK----------RQNTMVDHLLCLQETQPEYYTDNI 304

Query: 303 IIGQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLP 362
           I G M+ ++ AGTDTS  T+EW +S LLNHP +L+KA+ EID+  G  R + E+DL  LP
Sbjct: 305 IKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLP 364

Query: 363 FLQCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAA 422
           +LQ ++ E+LR+YP  PLLVPH +S DC V GYH+PRGTML+ NAWAIH D ++W+D  +
Sbjct: 365 YLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTS 424

Query: 423 FKPERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWER 482
           FKPERF            +G   K + FG+GRR CPG GLA R+  L +GSLIQCFEWER
Sbjct: 425 FKPERF----------EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWER 484

Query: 483 IGEEMVEMGE-GTGLTMPKACPLQAKCKPRPIL 509
           IGEE V+M E G G+ MPKA PL A CK RP++
Sbjct: 485 IGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVV 492

BLAST of Lsi04G023680 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 446.4 bits (1147), Expect = 3.0e-125
Identity = 238/509 (46.76%), Postives = 327/509 (64.24%), Query Frame = 0

Query: 3   TILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQY- 62
           T++   LF   +L  LI  L     N PPSP  +LP +GHL LLKPP+HRT   +S    
Sbjct: 5   TLIFSILFVVLSLIYLIGKLKRK-PNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 64

Query: 63  -GPILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGD 122
             PI  LR G+R V + SS S A++CF++ND+V ANRP  +  KH+ YD+T ++ A YGD
Sbjct: 65  NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 124

Query: 123 HWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQ--TVVDVRTVLFEFMLN 182
           HWRNLRRI S+ + S+  L +  S+R DE+  L+ RLS+N +Q    VD++++L +   N
Sbjct: 125 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFN 184

Query: 183 VMMRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKR 242
            ++RM+ GKR + D   D  E++  ++++ +    AGA N VDY P+L+   +    E R
Sbjct: 185 NILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLR---LVSDYETR 244

Query: 243 YINLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEII 302
              L  + ++ +Q L++E R  KE           K  TMI+ +L+LQES PDY+TD II
Sbjct: 245 VKKLAGRLDEFLQGLVDEKREAKE-----------KGNTMIDHLLTLQESQPDYFTDRII 304

Query: 303 IGQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPF 362
            G M+ ++ AGTDTS  T+EWA+S +LNHPDVL KA+ EID   G  R + E+D+  LP+
Sbjct: 305 KGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPY 364

Query: 363 LQCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAF 422
           LQ ++ ETLR+YP  P+L+PH +S DC V GY +PRGT+L+ N WAIH D +LW+D  +F
Sbjct: 365 LQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSF 424

Query: 423 KPERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERI 482
           KPERF            +G   K MPFG+GRR CPG GLA R++ L LGSLIQC EWE+I
Sbjct: 425 KPERF----------EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI 484

Query: 483 GEEMVEMGEGTGLTMPKACPLQAKCKPRP 507
           GEE V+M EG G+TMPKA PL+A C+ RP
Sbjct: 485 GEE-VDMSEGKGVTMPKAKPLEAMCRARP 487

BLAST of Lsi04G023680 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 445.3 bits (1144), Expect = 6.7e-125
Identity = 241/510 (47.25%), Postives = 326/510 (63.92%), Query Frame = 0

Query: 6   LYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQY--GP 65
           L+F F F +L LL       + N PPSP    P +GHLHLLK PLHR    +S+      
Sbjct: 17  LFFFFIFLSLKLLFGPRPRKL-NLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAK 76

Query: 66  ILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDHWR 125
           I  L  GSR V ++SS + A++CF++ND+V ANRP  L GKH+GY+ T +V A YGD WR
Sbjct: 77  IFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWR 136

Query: 126 NLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQ--TVVDVRTVLFEFMLNVMM 185
           NLRRI ++ + SS  L +  S+R DE+  LI+ L+KNS      V+++ +     +N ++
Sbjct: 137 NLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNII 196

Query: 186 RMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKRYIN 245
           RM+ GKR + D T +  E+++ ++++ E     GA N  DYFP+L++       EK    
Sbjct: 197 RMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRY---VTNYEKHVKK 256

Query: 246 LRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIIIGQ 305
           L  + ++ +Q+L+ E R EK           +K  TMI+ +LSLQE+ PDYYTD II G 
Sbjct: 257 LAGRVDEFLQSLVNEKRVEK-----------VKGNTMIDHLLSLQETQPDYYTDVIIKGI 316

Query: 306 MMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFLQC 365
           ++VM+ AGTDTS GT+EWAMS LLNHP+VL KAK EIDD  G  R + E D+ KLP+LQ 
Sbjct: 317 ILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQH 376

Query: 366 VIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFKPE 425
           ++ ETLR+YPV P+L+PH +S DC V GY +PRGT+++VNAWAIH D +LWE+   FKPE
Sbjct: 377 IVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPE 436

Query: 426 RFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIGEE 485
           RF             G   K MPFG+GRR CPG GLA R++ L LGSL+QCFEWER+ E+
Sbjct: 437 RF----------EKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEK 496

Query: 486 MVEMGEG-TGLTMPKACPLQAKCKPRPILY 510
            ++M E   G TM KA  LQA CK RPI++
Sbjct: 497 YLDMRESEKGTTMRKATSLQAMCKARPIVH 501

BLAST of Lsi04G023680 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 439.1 bits (1128), Expect = 4.8e-123
Identity = 238/517 (46.03%), Postives = 334/517 (64.60%), Query Frame = 0

Query: 2   VTILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQY 61
           + ++LY +F    L +    L     N PPSP   LP +GHL LLKPP+HRTL   S+  
Sbjct: 6   IRVVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 65

Query: 62  -----GPILLLRFGSRPVLLISSPS-AADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVW 121
                G ++ LR GSR V ++SS   AA++CF +ND+V ANRP+++ GKH+GY+ T ++ 
Sbjct: 66  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 125

Query: 122 APYGDHWRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSK--NSNQTVVDVRTVLF 181
           APYGDHWRNLRR+ ++ + S+  L     VR DEV  LI RLS+   + +TVV+++ +L 
Sbjct: 126 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 185

Query: 182 EFMLNVMMRMIDGKRCFNDNTTDTEESRNFQEIVTET-FKLAGANLVDYFPMLKWTGITW 241
           +   N +MRM+ GKR + + TTD EE++  +++V +     +  N VDY P+L+   +  
Sbjct: 186 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILR---LFS 245

Query: 242 KIEKRYINLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYY 301
             E R   L ++ +K +Q LI++ R ++E             TTMI+ +L LQ+SD +YY
Sbjct: 246 SYENRVKKLGEETDKFLQGLIDDKRGQQE-----------TGTTMIDHLLVLQKSDIEYY 305

Query: 302 TDEIIIGQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADL 361
           TD+II G +++M+ AGT+TS  T+EWA+S LLNHPDV++KA+ EID+  G  R I EADL
Sbjct: 306 TDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADL 365

Query: 362 EKLPFLQCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWE 421
            +LP+L+ ++ ETLR++P  PLLVPH +S DC +  Y +PRGT L+VNAWAIH D   W+
Sbjct: 366 SELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWD 425

Query: 422 DAAAFKPERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCF 481
           D  +FKPERF     A           K + FG+GRR CPG GLA R++GL LGSLIQCF
Sbjct: 426 DPDSFKPERFEKEEEAQ----------KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCF 485

Query: 482 EWERIGEEMVEMGEGTGLTMPKACPLQAKCKPRPILY 510
           EWER+G   V+M EG G T+PKA PL+A CK RP L+
Sbjct: 486 EWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLH 498

BLAST of Lsi04G023680 vs. TAIR 10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6 )

HSP 1 Score: 434.1 bits (1115), Expect = 1.6e-121
Identity = 236/512 (46.09%), Postives = 322/512 (62.89%), Query Frame = 0

Query: 4   ILLYFLFFFYALHLLIHHLLTNIHNRPPSPFPALPFLGHLHLLKPPLHRTLAKISDQYG- 63
           +LL   FF +    L+     +  N PPSP   LP +GHLHLLK PLHRT    S   G 
Sbjct: 21  LLLSLAFFLFISVKLLFGKRLSKFNLPPSPARPLPLIGHLHLLKLPLHRTFLSFSQSLGG 80

Query: 64  -PILLLRFGSRPVLLISSPSAADQCFSQNDIVFANRPRLLAGKHLGYDFTAVVWAPYGDH 123
            PI  LR G+R  +++SS S A++CF++NDIVFANRP L+ GKH+ Y+ T +  APYGDH
Sbjct: 81  APIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYGDH 140

Query: 124 WRNLRRISSLHLLSSANLQALSSVRADEVHSLILRLSKNSNQ--TVVDVRTVLFEFMLNV 183
           WRNLRRI++L + +S  L    SVR DE+  L+LRLSKNS      V++R +LFE  +N 
Sbjct: 141 WRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFELTINN 200

Query: 184 MMRMIDGKRCFNDNTTDTEESRNFQEIVTETFKLAGA-NLVDYFPMLKWTGITWKIEKRY 243
           + RM+ GKR + + T   E ++  + ++ E    AGA N  DY P+++W       EKR 
Sbjct: 201 VFRMVAGKRFYGEGTEQDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRW---FTNFEKRV 260

Query: 244 INLRKKRNKLIQNLIEEHRREKEKMMTNNNKSPLKKTTMIEVMLSLQESDPDYYTDEIII 303
            NL  + +K +Q+L++E R +KE           K TTMI+ +LSLQES PDYYTD  + 
Sbjct: 261 KNLAIRIDKFLQSLVDEKRADKE-----------KGTTMIDHLLSLQESQPDYYTDVTLK 320

Query: 304 GQMMVMLSAGTDTSVGTMEWAMSLLLNHPDVLAKAKAEIDDIAGEKRHIVEADLEKLPFL 363
           G ++VM+ AG++T   T+EWAM  +LNHP+VL KA+ EID   G  R + EAD + LP+L
Sbjct: 321 GIIIVMIIAGSETIAWTLEWAMLNVLNHPEVLKKARTEIDTKIGFDRLMDEADTKNLPYL 380

Query: 364 QCVIKETLRMYPVGPLLVPHESSADCTVVGYHIPRGTMLVVNAWAIHNDARLWEDAAAFK 423
           Q ++ ETLR++P  P  VPH +S DC + GY +PRG+ML+VN W++H D  +WED   FK
Sbjct: 381 QWIVLETLRLHPAAPTNVPHSTSEDCMLAGYDVPRGSMLLVNIWSMHRDPSIWEDPEMFK 440

Query: 424 PERFLGASAAAVGEVGDGIGLKYMPFGVGRRGCPGEGLALRVMGLVLGSLIQCFEWERIG 483
           PERF            + +  K + FG+GRR CPG GLA RV+ L LGS++QCFEW+RIG
Sbjct: 441 PERF----------KNEKLNQKLLSFGMGRRACPGYGLAHRVVSLTLGSMVQCFEWQRIG 500

Query: 484 EEMVEMGEG-TGLTMPKACPLQAKCKPRPILY 510
           +E V+  E  T + M    PL A CK RPI++
Sbjct: 501 QEYVDNSEDKTVVLMRPTTPLLAMCKARPIVH 508