Lsi04G023610 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G023610
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Glucan endo-1,3-beta-glucosidase
Location	chr04: 30780969 .. 30782948 (+)
RNA-Seq Expression	Lsi04G023610
Synteny	Lsi04G023610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGTTTGGCGCGGTTGGAGTGAACTATGGGTTGAACGGAGACAATCTTCCGAAAGCGAATGAAGTGATAAATCTATATGAACGATGTGGGATAAATATTGTGAGGATATTTGAGCCTAACCATGAGGTTCTTGATGCTTTACGAGGTAAGAGAAATTTAGTGTTGTGGTTAGGGACCAAAAATGAAGACATAAAGGCTTTTGCTACAAACCAATTAGCGGCCGATGCTTGGGTTAATGCTAATGTGGTTCCTTATTATAAGGATCTCAACATTCATTATATCACCGTCGGCAATGACCTTGTCCCTTCCGATGCAGCTTCACCATTTCTCGCCAATGCCATTCAAAATATTATGCATGCACTTCTCAATGCCCTCCTCATAACTGACATTAAGGTCAAATGTTTCCATTTCATCTTTATCATTCTTAATGATCCAACATCTAACCTCCAATTTACATACAGGTGACGACAGTGGTAGCCATGACAGTATTGGAAGTCTCGTCCCCACCTTCTGCTGGAGCATTCACACCCGCCGCCGCTGGAGCAATGAAAGACATTGCAAACGTGTTGGGATCTTCGGGCGCACCAATATTGATGAACGTGTACCCATATTTTGCGTACGCATCAAACCCACAACAAATCTCACTCGATTACGCGCTTTTCACATCCACCACACCCGTCGTGGTGGACGGAGATTTACAGTATTTAAATTTGTTTGACGCCATGGTGGACTCCTTCTATGCCGCACTGGAGAAGATTGACGCTGGGGGAGTGAGAATCGAGATCTCGGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACAAGCGTTGATAATGCATTGACTTATAACAAAAACTTTGTGAAACATGTGACCAGTGGAGTTGGAACCCCTAAAAGGCCTAATATCAAATACGATGTCGTTTTTTTTGAAATGTTCAATGAGAATCTTAAGACGTTTGGAGTTGAGCAAAATTTTGGCTTCTTTTATCCCAATATGAACCCAGTTTATCCCTTTTGGAATTGTTCACCCCCCACCCCCCCCTCCCAACTAGAATGTTTATTATTATTATTTTTTAAGAAAATGAGGGTTTTGGTCTTGTCACAATCCAACCTATATCATAATGTTTCCATACTATTTATTTATTTGAATTCAAAAGTTTCTTTTGAGATGACATAACTGATATTTTATCCACTAACTTACGTGATTGAAATACATGTTTACGCACGTTTTTTTTTCTTGAGAAAACCTCCATGCATATTATATATGTATTTGCAGTCACATTAATCACAAGTTTGTAATGTGACAAACTTTTTATGGTCTAGATGGTCATTCGAAGTCTATTATTAGTAAACTTTGCTATATTTATAATTTTTAAAAATATTACTATACATTTAATTATTATTTTTAAAATTATTATTCATTACAATTACCCAAAATTGTACGGTATGCAATCCATGGGTTATGGAGGAAGAATTGACAGTAGAATGGCTAACCAACCTGGATGGATCTTCACTCTTTTGGGTCAGGAGGGCAAATTGGGCTTGGGTTGGTACAGGCCCATTTGCCCTTAAAGATTATTATACCCGCAGTAATATAATTAAAAAAAAAAAAGAAAATACATTTTTAATCCTTGATTTTTCAATAATACGTGCAGTTGATCCTTAAGTTTTGAGAAATAGATTTAAAAAAATGTTTTTCATTTATTACAATTTTCTTTTATTATTTTTAATTTTGACATGACATTTTTAAATTAAAAAAAAGAAAAAAAAGTGCTCTCTCATCTATCAATCGAGGAAGAAAGAGAAGATGATTACGTTCCTTATTTGGCTGATTGTGTTTGTTTGGATGAATTTGTATAACTGAAGAAGCTCTTTTTCCAGCGAGGACATTTTATTCTTTACAAATTTTGCTAATTTGTCAGTAATTTTTTTAGGTTAAAATCACCATTCTAGTTTTATTGTTGGAACAGCTTAAT

mRNA sequence

GGGTTTGGCGCGGTTGGAGTGAACTATGGGTTGAACGGAGACAATCTTCCGAAAGCGAATGAAGTGATAAATCTATATGAACGATGTGGGATAAATATTGTGAGGATATTTGAGCCTAACCATGAGGTTCTTGATGCTTTACGAGGGACCAAAAATGAAGACATAAAGGCTTTTGCTACAAACCAATTAGCGGCCGATGCTTGGGTTAATGCTAATGTGGTTCCTTATTATAAGGATCTCAACATTCATTATATCACCGTCGGCAATGACCTTGTCCCTTCCGATGCAGCTTCACCATTTCTCGCCAATGCCATTCAAAATATTATGCATGCACTTCTCAATGCCCTCCTCATAACTGACATTAAGGTGACGACAGTGGTAGCCATGACAGTATTGGAAGTCTCGTCCCCACCTTCTGCTGGAGCATTCACACCCGCCGCCGCTGGAGCAATGAAAGACATTGCAAACGTGTTGGGATCTTCGGGCGCACCAATATTGATGAACGTGTACCCATATTTTGCGTACGCATCAAACCCACAACAAATCTCACTCGATTACGCGCTTTTCACATCCACCACACCCGTCGTGGTGGACGGAGATTTACAGTATTTAAATTTGTTTGACGCCATGGTGGACTCCTTCTATGCCGCACTGGAGAAGATTGACGCTGGGGGAGTGAGAATCGAGATCTCGGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACAAGCGTTGATAATGCATTGACTTATAACAAAAACTTTGTGAAACATGTGACCAGTGGAGTTGGAACCCCTAAAAGGCCTAATATCAAATACGATGTCGTTTTTTTTGAAATGTTCAATGAGAATCTTAAGACGTTTGGAGTTGAGCAAAATTTTGGCTTCTTTTATCCCAATATGAACCCAGTTAAAATCACCATTCTAGTTTTATTGTTGGAACAGCTTAAT

Coding sequence (CDS)

GGGTTTGGCGCGGTTGGAGTGAACTATGGGTTGAACGGAGACAATCTTCCGAAAGCGAATGAAGTGATAAATCTATATGAACGATGTGGGATAAATATTGTGAGGATATTTGAGCCTAACCATGAGGTTCTTGATGCTTTACGAGGGACCAAAAATGAAGACATAAAGGCTTTTGCTACAAACCAATTAGCGGCCGATGCTTGGGTTAATGCTAATGTGGTTCCTTATTATAAGGATCTCAACATTCATTATATCACCGTCGGCAATGACCTTGTCCCTTCCGATGCAGCTTCACCATTTCTCGCCAATGCCATTCAAAATATTATGCATGCACTTCTCAATGCCCTCCTCATAACTGACATTAAGGTGACGACAGTGGTAGCCATGACAGTATTGGAAGTCTCGTCCCCACCTTCTGCTGGAGCATTCACACCCGCCGCCGCTGGAGCAATGAAAGACATTGCAAACGTGTTGGGATCTTCGGGCGCACCAATATTGATGAACGTGTACCCATATTTTGCGTACGCATCAAACCCACAACAAATCTCACTCGATTACGCGCTTTTCACATCCACCACACCCGTCGTGGTGGACGGAGATTTACAGTATTTAAATTTGTTTGACGCCATGGTGGACTCCTTCTATGCCGCACTGGAGAAGATTGACGCTGGGGGAGTGAGAATCGAGATCTCGGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACAAGCGTTGATAATGCATTGACTTATAACAAAAACTTTGTGAAACATGTGACCAGTGGAGTTGGAACCCCTAAAAGGCCTAATATCAAATACGATGTCGTTTTTTTTGAAATGTTCAATGAGAATCTTAAGACGTTTGGAGTTGAGCAAAATTTTGGCTTCTTTTATCCCAATATGAACCCAGTTAAAATCACCATTCTAGTTTTATTGTTGGAACAGCTTAAT

Protein sequence

GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALRGTKNEDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPYFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVEQNFGFFYPNMNPVKITILVLLLEQLN

Homology

[Close]

BLAST of Lsi04G023610 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 279.6 bits (714), Expect = 4.3e-74
Identity = 144/310 (46.45%), Postives = 199/310 (64.19%), Query Frame = 0


Query: 4   AVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALRGTK--------NEDI 63
           ++GV YG+ G+NLP  +EV++LY+   I  +R+++PN   L ALR +           D+
Sbjct: 28  SIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDV 87

Query: 64  KAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLNA 123
           ++ A+N  AA  W+  NVV Y+  ++  YI VGN+L+P    + ++  A++NI +AL +A
Sbjct: 88  QSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSA 147

Query: 124 LLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPYFAY 183
            L   IKV+T V   VL  S PPSAGAF+ AA   +  I   L S+GAP+L+NVYPYF+Y
Sbjct: 148 GLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSY 207

Query: 184 ASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISETGW 243
             NP QISL YALFT++  VV DG   Y NLFDA+VD+ +AALE++    V + +SE+GW
Sbjct: 208 TGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGW 267

Query: 244 PTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVEQNF 303
           P+ G     S  NA TYN+N ++HV  G GTP+RP  + +   FEMFNEN K  G+EQNF
Sbjct: 268 PSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNF 327

Query: 304 GFFYPNMNPV 306
           G FYPN  PV
Sbjct: 328 GLFYPNKQPV 335

[Close]

BLAST of Lsi04G023610 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-70
Identity = 146/314 (46.50%), Postives = 197/314 (62.74%), Query Frame = 0


Query: 4   AVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKNED 63
           ++G+NYGL GDNLP  + VINLY+  GI+ +RIF+PN EVL+ALR         G K++D
Sbjct: 33  SIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQD 92

Query: 64  IKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLN 123
           + A A ++ A   W  AN+  Y  D+NI +ITVGN+++P       L      +M +L N
Sbjct: 93  LAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLP-----VMQSLTN 152

Query: 124 ALLITD--IKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 183
            +   +  I ++TVVAM+ LE S PPSAG FT  A   +  +  +L  +  PIL+N+YPY
Sbjct: 153 LVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY 212

Query: 184 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 243
           FAYAS+P  I LDYA F + + VV DG L Y N+FDA+ D+F  A+EK     + + +SE
Sbjct: 213 FAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSE 272

Query: 244 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 303
           TGWP+ GN  FT+   A TYN+NFVKH+ SG GTPKRPN   +   F  FNEN K  G E
Sbjct: 273 TGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTE 332

Query: 304 QNFGFFYP-NMNPV 306
           QNFG + P +M P+
Sbjct: 333 QNFGLYNPSDMKPI 341

[Close]

BLAST of Lsi04G023610 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.1e-69
Identity = 142/314 (45.22%), Postives = 193/314 (61.46%), Query Frame = 0


Query: 4   AVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKNED 63
           ++G+NYGL GDNLP  ++VI LY+   I  +RIF+PN EVL+ALR         G +++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 64  IKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLN 123
           + A + ++ A   W   N+ PY  D+NI +ITVGN+++P       L      +M +L N
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLP-----VMQSLTN 161

Query: 124 ALLITD--IKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 183
            +   +  I ++TVVAM  LE S PPSAG FT  A   +  +  +L  + +PIL+ +YPY
Sbjct: 162 LVKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPY 221

Query: 184 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 243
           F+YAS+P  I LDYA F +   VV DG L Y N+FDA+ D+F  A+EK     + + +SE
Sbjct: 222 FSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSE 281

Query: 244 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 303
           TGWP+ GN   T+ D A TYN+NFVKH+ SG GTPKRPN   D   F  FNEN K  G E
Sbjct: 282 TGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTE 341

Query: 304 QNFGFFYPN-MNPV 306
           QNFG + PN M P+
Sbjct: 342 QNFGLYNPNDMKPI 350

[Close]

BLAST of Lsi04G023610 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.2e-61
Identity = 136/315 (43.17%), Postives = 194/315 (61.59%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR--------GTKN 60
           G   +GVNYG+ G +LP  ++V+ LY+   I  VRIF P+  VL+ALR        GT N
Sbjct: 3   GVEGIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALRNSGLGVVLGTLN 62

Query: 61  EDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHAL 120
            D+   A++   A +WV++ V P+   ++  YI  GN+++P ++A+  L  A++N+  AL
Sbjct: 63  SDLAPLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAALVLP-AMKNLEAAL 122

Query: 121 LNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 180
             A L   + VTT +A +VL  S PPS G F+ AA   +  I + L SSG P+L+NVYPY
Sbjct: 123 QAAGL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVYPY 182

Query: 181 FAYASNPQQISLDYALFTSTTPV-VVDGDLQYLNLFDAMVDSFYAALEKIDAG-GVRIEI 240
           FAY+++P  + LDYAL +S+  V V D  ++Y N+FDA++D+ YAA+EK   G  + + +
Sbjct: 183 FAYSADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLELVV 242

Query: 241 SETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFG 300
           SETGWP+ G     SV+NA  Y  N V+HV    GTP+RP    +   F MFNEN K  G
Sbjct: 243 SETGWPSGGGGYGASVENAAAYINNLVRHVG---GTPRRPGKAVETYIFAMFNENQKPEG 302

Query: 301 VEQNFGFFYPNMNPV 306
           VEQNFG F P+M+ V
Sbjct: 303 VEQNFGMFQPDMSQV 311

[Close]

BLAST of Lsi04G023610 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 236.1 bits (601), Expect = 5.4e-61
Identity = 132/312 (42.31%), Postives = 193/312 (61.86%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALRGT--------KN 60
           G  ++GV YG NG+NLP A++VINLY+  GI  +RI++PN + L ALRG+         N
Sbjct: 26  GAQSIGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSDIELILDVPN 85

Query: 61  EDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDA---ASPFLANAIQNIM 120
            D+++ A++  AA  WV  NVV Y  ++   YI VGN+++P+ +    + ++  A++N+ 
Sbjct: 86  TDLQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNAQYAQYVLPAMKNVQ 145

Query: 121 HALLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNV 180
            A+ +A L   IKV+T     VL  S PPS G+F+   +  +  I + L  +G+P+L N+
Sbjct: 146 SAITSAGLQDQIKVSTATFSAVLGKSYPPSEGSFSDDVSSFINPIISFLAENGSPLLANI 205

Query: 181 YPYFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIE 240
           YPYF+Y  + Q I LDYALFT++  VV DG  QY NLFDA++D+ YAALEK     ++I 
Sbjct: 206 YPYFSYTGDTQNIRLDYALFTASGVVVQDGSYQYQNLFDALLDALYAALEKAGGSNLKIV 265

Query: 241 ISETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKT- 300
           +SE+GWP++G    T VDNA TY KN + HV    GTP++     +   F MF+EN KT 
Sbjct: 266 VSESGWPSEGGTAAT-VDNARTYYKNLINHVKG--GTPRKSG-AIETYLFAMFDENQKTG 325

[Close]

BLAST of Lsi04G023610 vs. ExPASy TrEMBL
Match: A0A5A7V7B6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G002240 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 255/313 (81.47%), Postives = 275/313 (87.86%), Query Frame = 0


Query: 2   FGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKN 61
           FGAVGVNYGLN DNLPK +EVINLYERCGINIVRIFEPNHEVLDALR         GT+N
Sbjct: 26  FGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTRN 85

Query: 62  EDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHAL 121
           EDIK FATNQ  ADAWVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAIQNIM AL
Sbjct: 86  EDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQAL 145

Query: 122 LNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 181
           +NA + +DIKVTTVVAMT LEVSSPPSAGAFT  AA AMKDI +VL SS APILMNVYPY
Sbjct: 146 VNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYPY 205

Query: 182 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 241
           FAYASNPQQI L YALFTSTTPVVVDGDL+Y NL DAMVDSFY ALEKI   GVRI ISE
Sbjct: 206 FAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGISE 265

Query: 242 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 301
           TGWP+KGNEPFTSVDNALTYN+NFVKHV+SGVGTPK PN++YD+V FEMFNE+LK+ GVE
Sbjct: 266 TGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVLFEMFNEDLKSPGVE 325

Query: 302 QNFGFFYPNMNPV 306
           QNFGFFYPNMNPV
Sbjct: 326 QNFGFFYPNMNPV 338

[Close]

BLAST of Lsi04G023610 vs. ExPASy TrEMBL
Match: A0A5D3D504 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001690 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 255/313 (81.47%), Postives = 275/313 (87.86%), Query Frame = 0


Query: 2   FGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKN 61
           FGAVGVNYGLN DNLPK +EVINLYERCGINIVRIFEPNHEVLDALR         GT+N
Sbjct: 26  FGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTRN 85

Query: 62  EDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHAL 121
           EDIK FATNQ  ADAWVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAIQNIM AL
Sbjct: 86  EDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQAL 145

Query: 122 LNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 181
           +NA + +DIKVTTVVAMT LEVSSPPSAGAFT  AA AMKDI +VL SS APILMNVYPY
Sbjct: 146 VNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYPY 205

Query: 182 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 241
           FAYASNPQQI L YALFTSTTPVVVDGDL+Y NL DAMVDSFY ALEKI   GVRI ISE
Sbjct: 206 FAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGISE 265

Query: 242 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 301
           TGWP+KGNEPFTSVDNALTYN+NFVKHV+SGVGTPK PN++YD+V FEMFNE+LK+ GVE
Sbjct: 266 TGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVLFEMFNEDLKSPGVE 325

Query: 302 QNFGFFYPNMNPV 306
           QNFGFFYPNMNPV
Sbjct: 326 QNFGFFYPNMNPV 338

[Close]

BLAST of Lsi04G023610 vs. ExPASy TrEMBL
Match: A0A5D3D518 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001700 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.6e-138
Identity = 252/314 (80.25%), Postives = 276/314 (87.90%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTK 60
           G G +GVNYGLNGDNLP  ++VINLY RCGINIVR+FEPNH VLDAL+         GT+
Sbjct: 25  GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 84

Query: 61  NEDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHA 120
           NEDI+ FATNQLAA+ WVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAI+NIM A
Sbjct: 85  NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 144

Query: 121 LLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYP 180
           L+N  + +DIKVTTVVAMT LEVS PPSAGAFT +AAG MKDIANVLGSSGAPIL+NVYP
Sbjct: 145 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 204

Query: 181 YFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEIS 240
           YFAYASNPQQISL+YALFTSTTPVVVDGDLQY NLFDAMVDSFYAALEKIDA  +RI IS
Sbjct: 205 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 264

Query: 241 ETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGV 300
           ETGWPT GNEPFTSVDNALTYNKN V HVTSGVGTPKRPN+KYD V FEMFNE+LK  GV
Sbjct: 265 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 324

Query: 301 EQNFGFFYPNMNPV 306
           EQNFGFF+PNMNPV
Sbjct: 325 EQNFGFFFPNMNPV 338

[Close]

BLAST of Lsi04G023610 vs. ExPASy TrEMBL
Match: A0A1S4DV56 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.6e-138
Identity = 252/314 (80.25%), Postives = 276/314 (87.90%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTK 60
           G G +GVNYGLNGDNLP  ++VINLY RCGINIVR+FEPNH VLDAL+         GT+
Sbjct: 333 GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 392

Query: 61  NEDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHA 120
           NEDI+ FATNQLAA+ WVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAI+NIM A
Sbjct: 393 NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 452

Query: 121 LLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYP 180
           L+N  + +DIKVTTVVAMT LEVS PPSAGAFT +AAG MKDIANVLGSSGAPIL+NVYP
Sbjct: 453 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 512

Query: 181 YFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEIS 240
           YFAYASNPQQISL+YALFTSTTPVVVDGDLQY NLFDAMVDSFYAALEKIDA  +RI IS
Sbjct: 513 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 572

Query: 241 ETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGV 300
           ETGWPT GNEPFTSVDNALTYNKN V HVTSGVGTPKRPN+KYD V FEMFNE+LK  GV
Sbjct: 573 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 632

Query: 301 EQNFGFFYPNMNPV 306
           EQNFGFF+PNMNPV
Sbjct: 633 EQNFGFFFPNMNPV 646

[Close]

BLAST of Lsi04G023610 vs. ExPASy TrEMBL
Match: A0A0A0L3A5 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.1e-136
Identity = 248/314 (78.98%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTK 60
           GFG +GVNYGLNGDNLP  ++VINLY RCGINI+R+FEPNH VLDAL+         GT+
Sbjct: 25  GFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTR 84

Query: 61  NEDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHA 120
           NEDI+ FATNQLAA+AWVNANVVPYYK++NI YIT+GN++VP DAA+PF+AN I+NIM A
Sbjct: 85  NEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQA 144

Query: 121 LLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYP 180
           L++  + +DIKVTTVVAMT L VS PPSAGAFT +AAG MKDIANVLGSSGAPIL+NVYP
Sbjct: 145 LVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYP 204

Query: 181 YFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEIS 240
           YFAYASNPQQISL+YALFTS+TPVVVDG+LQY NLFDAMVDSFYAALEKIDAG +RI IS
Sbjct: 205 YFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGIS 264

Query: 241 ETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGV 300
           ETGWPT GNEPFTSV+NALTYNKN VKHVTSGVGTPKRPN+KYDVV FEMFNE+LK  GV
Sbjct: 265 ETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGV 324

Query: 301 EQNFGFFYPNMNPV 306
           EQNFGFF PNMNPV
Sbjct: 325 EQNFGFFSPNMNPV 338

[Close]

BLAST of Lsi04G023610 vs. NCBI nr
Match: XP_038896614.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 504.6 bits (1298), Expect = 6.2e-139
Identity = 253/314 (80.57%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTK 60
           G G VGVNYGLNGDNLP  ++VINLY RCGINI+RIFEPNH VLDAL+         GT+
Sbjct: 30  GSGTVGVNYGLNGDNLPIPSDVINLYGRCGINIIRIFEPNHGVLDALKGQANLVLWLGTR 89

Query: 61  NEDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHA 120
           NEDI+ FAT+Q+AA+AWVNANVVPYYKD+NI YITVGN++VP+DAA+PF+ANAI NIM A
Sbjct: 90  NEDIEGFATDQVAANAWVNANVVPYYKDVNIAYITVGNEVVPADAAAPFVANAITNIMQA 149

Query: 121 LLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYP 180
           L+NA + +DIKVTTVVAMT LEVS PPSAGAFT  AAG MKDIANVLGSSGAPIL+NVYP
Sbjct: 150 LVNAGIQSDIKVTTVVAMTALEVSYPPSAGAFTATAAGVMKDIANVLGSSGAPILVNVYP 209

Query: 181 YFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEIS 240
           YFAYASNP+QISL+YALFTS+TPVVVDGDL Y NLFDAMVDSFYAALEKIDAGGV I IS
Sbjct: 210 YFAYASNPEQISLNYALFTSSTPVVVDGDLHYFNLFDAMVDSFYAALEKIDAGGVGIAIS 269

Query: 241 ETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGV 300
           ETGWPT GNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPN+ YD + FEMFNE+LK  GV
Sbjct: 270 ETGWPTDGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNVFYDAILFEMFNEDLKAPGV 329

Query: 301 EQNFGFFYPNMNPV 306
           EQNFGFFYPNMNPV
Sbjct: 330 EQNFGFFYPNMNPV 343

[Close]

BLAST of Lsi04G023610 vs. NCBI nr
Match: KAA0064262.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 502.7 bits (1293), Expect = 2.4e-138
Identity = 255/313 (81.47%), Postives = 275/313 (87.86%), Query Frame = 0


Query: 2   FGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKN 61
           FGAVGVNYGLN DNLPK +EVINLYERCGINIVRIFEPNHEVLDALR         GT+N
Sbjct: 26  FGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTRN 85

Query: 62  EDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHAL 121
           EDIK FATNQ  ADAWVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAIQNIM AL
Sbjct: 86  EDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQAL 145

Query: 122 LNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 181
           +NA + +DIKVTTVVAMT LEVSSPPSAGAFT  AA AMKDI +VL SS APILMNVYPY
Sbjct: 146 VNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYPY 205

Query: 182 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 241
           FAYASNPQQI L YALFTSTTPVVVDGDL+Y NL DAMVDSFY ALEKI   GVRI ISE
Sbjct: 206 FAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGISE 265

Query: 242 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 301
           TGWP+KGNEPFTSVDNALTYN+NFVKHV+SGVGTPK PN++YD+V FEMFNE+LK+ GVE
Sbjct: 266 TGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVLFEMFNEDLKSPGVE 325

Query: 302 QNFGFFYPNMNPV 306
           QNFGFFYPNMNPV
Sbjct: 326 QNFGFFYPNMNPV 338

[Close]

BLAST of Lsi04G023610 vs. NCBI nr
Match: TYK18631.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 502.7 bits (1293), Expect = 2.4e-138
Identity = 255/313 (81.47%), Postives = 275/313 (87.86%), Query Frame = 0


Query: 2   FGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKN 61
           FGAVGVNYGLN DNLPK +EVINLYERCGINIVRIFEPNHEVLDALR         GT+N
Sbjct: 26  FGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTRN 85

Query: 62  EDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHAL 121
           EDIK FATNQ  ADAWVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAIQNIM AL
Sbjct: 86  EDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQAL 145

Query: 122 LNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 181
           +NA + +DIKVTTVVAMT LEVSSPPSAGAFT  AA AMKDI +VL SS APILMNVYPY
Sbjct: 146 VNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYPY 205

Query: 182 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 241
           FAYASNPQQI L YALFTSTTPVVVDGDL+Y NL DAMVDSFY ALEKI   GVRI ISE
Sbjct: 206 FAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGISE 265

Query: 242 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 301
           TGWP+KGNEPFTSVDNALTYN+NFVKHV+SGVGTPK PN++YD+V FEMFNE+LK+ GVE
Sbjct: 266 TGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVLFEMFNEDLKSPGVE 325

Query: 302 QNFGFFYPNMNPV 306
           QNFGFFYPNMNPV
Sbjct: 326 QNFGFFYPNMNPV 338

[Close]

BLAST of Lsi04G023610 vs. NCBI nr
Match: XP_016899853.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo])

HSP 1 Score: 501.5 bits (1290), Expect = 5.3e-138
Identity = 252/314 (80.25%), Postives = 276/314 (87.90%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTK 60
           G G +GVNYGLNGDNLP  ++VINLY RCGINIVR+FEPNH VLDAL+         GT+
Sbjct: 333 GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 392

Query: 61  NEDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHA 120
           NEDI+ FATNQLAA+ WVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAI+NIM A
Sbjct: 393 NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 452

Query: 121 LLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYP 180
           L+N  + +DIKVTTVVAMT LEVS PPSAGAFT +AAG MKDIANVLGSSGAPIL+NVYP
Sbjct: 453 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 512

Query: 181 YFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEIS 240
           YFAYASNPQQISL+YALFTSTTPVVVDGDLQY NLFDAMVDSFYAALEKIDA  +RI IS
Sbjct: 513 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 572

Query: 241 ETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGV 300
           ETGWPT GNEPFTSVDNALTYNKN V HVTSGVGTPKRPN+KYD V FEMFNE+LK  GV
Sbjct: 573 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 632

Query: 301 EQNFGFFYPNMNPV 306
           EQNFGFF+PNMNPV
Sbjct: 633 EQNFGFFFPNMNPV 646

[Close]

BLAST of Lsi04G023610 vs. NCBI nr
Match: TYK18632.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 501.5 bits (1290), Expect = 5.3e-138
Identity = 252/314 (80.25%), Postives = 276/314 (87.90%), Query Frame = 0


Query: 1   GFGAVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTK 60
           G G +GVNYGLNGDNLP  ++VINLY RCGINIVR+FEPNH VLDAL+         GT+
Sbjct: 25  GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 84

Query: 61  NEDIKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHA 120
           NEDI+ FATNQLAA+ WVNANVVPYYKD+NI YITVGN++VP DAA+PF+ANAI+NIM A
Sbjct: 85  NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 144

Query: 121 LLNALLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYP 180
           L+N  + +DIKVTTVVAMT LEVS PPSAGAFT +AAG MKDIANVLGSSGAPIL+NVYP
Sbjct: 145 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 204

Query: 181 YFAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEIS 240
           YFAYASNPQQISL+YALFTSTTPVVVDGDLQY NLFDAMVDSFYAALEKIDA  +RI IS
Sbjct: 205 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 264

Query: 241 ETGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGV 300
           ETGWPT GNEPFTSVDNALTYNKN V HVTSGVGTPKRPN+KYD V FEMFNE+LK  GV
Sbjct: 265 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 324

Query: 301 EQNFGFFYPNMNPV 306
           EQNFGFF+PNMNPV
Sbjct: 325 EQNFGFFFPNMNPV 338

[Close]

BLAST of Lsi04G023610 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 274.6 bits (701), Expect = 9.8e-74
Identity = 141/308 (45.78%), Postives = 206/308 (66.88%), Query Frame = 0


Query: 6   GVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALRGT--------KNEDIKA 65
           GV YG NGDNLP   + ++LY++  +  +R++EP  +++ +L+GT        +NE IK 
Sbjct: 25  GVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQGTGLLVAIGPRNEAIKT 84

Query: 66  FATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLNALL 125
            A     A  W    + P YK++  ++ITVGN+++  +    ++  A++NI  A L  + 
Sbjct: 85  LAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGEIGR-YVPQAMKNI-KAALTEIG 144

Query: 126 ITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPYFAYAS 185
            + I VTTV++   L  S PPSAG F PA    + +I ++L S+ +P+++NVYPYFAYAS
Sbjct: 145 NSKIHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLMVNVYPYFAYAS 204

Query: 186 NPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISETGWPT 245
           +P  +SL+YA F ST+PVV DG  QY N+FDA +D+F  ALEKI+ G V++ ++ETGWPT
Sbjct: 205 DPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSVKVYVAETGWPT 264

Query: 246 KGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVEQNFGF 305
           +GN+P+TSV+NA  YN+  +K +T+G GTP+RPN+     FFEMFNE+LK   VEQ+FGF
Sbjct: 265 RGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDLKQGAVEQSFGF 324

[Close]

BLAST of Lsi04G023610 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 268.1 bits (684), Expect = 9.2e-72
Identity = 146/314 (46.50%), Postives = 197/314 (62.74%), Query Frame = 0


Query: 4   AVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKNED 63
           ++G+NYGL GDNLP  + VINLY+  GI+ +RIF+PN EVL+ALR         G K++D
Sbjct: 33  SIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQD 92

Query: 64  IKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLN 123
           + A A ++ A   W  AN+  Y  D+NI +ITVGN+++P       L      +M +L N
Sbjct: 93  LAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLP-----VMQSLTN 152

Query: 124 ALLITD--IKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 183
            +   +  I ++TVVAM+ LE S PPSAG FT  A   +  +  +L  +  PIL+N+YPY
Sbjct: 153 LVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY 212

Query: 184 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 243
           FAYAS+P  I LDYA F + + VV DG L Y N+FDA+ D+F  A+EK     + + +SE
Sbjct: 213 FAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSE 272

Query: 244 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 303
           TGWP+ GN  FT+   A TYN+NFVKH+ SG GTPKRPN   +   F  FNEN K  G E
Sbjct: 273 TGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTE 332

Query: 304 QNFGFFYP-NMNPV 306
           QNFG + P +M P+
Sbjct: 333 QNFGLYNPSDMKPI 341

[Close]

BLAST of Lsi04G023610 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.5e-71
Identity = 145/307 (47.23%), Postives = 197/307 (64.17%), Query Frame = 0


Query: 9   YGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALRGT--------KNEDIKAFAT 68
           YG NG+NLP   + + LY+   I+ +R++EP  ++L+ALRG+        +NEDI++ A 
Sbjct: 34  YGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADMLEALRGSGLLVAFGPRNEDIQSLAH 93

Query: 69  NQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLNALLITD 128
           +  AA  +V+  + PY  D+ I +IT+GN++ P + A  F+A AI+N+  AL N+  +T 
Sbjct: 94  DPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGEIAQ-FVAAAIKNVNVALTNS-GVTG 153

Query: 129 IKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPYFAYASNPQ 188
           I VTTV+AMT L  + PPSA  F P     M +I ++L  + +P++ N+YPYFAYAS+P 
Sbjct: 154 ISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITSILSETNSPLMTNIYPYFAYASDPY 213

Query: 189 QISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISETGWPTKGN 248
            ISLDYA F S TPVV+DGDL Y N+F+AMVD F AALEKI+A  V + ++ETGWPT+GN
Sbjct: 214 HISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNAALEKINAANVVVMVAETGWPTEGN 273

Query: 249 EPFTSVDNALTYNKNF--VKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVEQNFGFF 306
            P TSVDNA  YN              TP+R N   DV  F MF EN K   VEQ+FG F
Sbjct: 274 PPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPVDVFLFAMFKENQKDGPVEQSFGIF 333

[Close]

BLAST of Lsi04G023610 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 262.3 bits (669), Expect = 5.0e-70
Identity = 142/314 (45.22%), Postives = 193/314 (61.46%), Query Frame = 0


Query: 4   AVGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALR---------GTKNED 63
           ++G+NYGL GDNLP  ++VI LY+   I  +RIF+PN EVL+ALR         G +++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 64  IKAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLN 123
           + A + ++ A   W   N+ PY  D+NI +ITVGN+++P       L      +M +L N
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLP-----VMQSLTN 161

Query: 124 ALLITD--IKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPY 183
            +   +  I ++TVVAM  LE S PPSAG FT  A   +  +  +L  + +PIL+ +YPY
Sbjct: 162 LVKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPY 221

Query: 184 FAYASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISE 243
           F+YAS+P  I LDYA F +   VV DG L Y N+FDA+ D+F  A+EK     + + +SE
Sbjct: 222 FSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSE 281

Query: 244 TGWPTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVE 303
           TGWP+ GN   T+ D A TYN+NFVKH+ SG GTPKRPN   D   F  FNEN K  G E
Sbjct: 282 TGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTE 341

Query: 304 QNFGFFYPN-MNPV 306
           QNFG + PN M P+
Sbjct: 342 QNFGLYNPNDMKPI 350

[Close]

BLAST of Lsi04G023610 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.4e-67
Identity = 134/305 (43.93%), Postives = 184/305 (60.33%), Query Frame = 0


Query: 5   VGVNYGLNGDNLPKANEVINLYERCGINIVRIFEPNHEVLDALRGT---------KNEDI 64
           VG+NYGL GDNLP  + VI  Y+   +  +RIFEPN +VL+ALRG          KNED+
Sbjct: 34  VGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNEDL 93

Query: 65  KAFATNQLAADAWVNANVVPYYKDLNIHYITVGNDLVPSDAASPFLANAIQNIMHALLNA 124
           +A A N+ A  +W + N+ PY  D+NI +ITVGN  +P D   P +   IQ++   + + 
Sbjct: 94  EALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKSR 153

Query: 125 LLITDIKVTTVVAMTVLEVSSPPSAGAFTPAAAGAMKDIANVLGSSGAPILMNVYPYFAY 184
            L   I ++T V  T L    PPSAG  TP A   +  +  +L  +  PI +N+YPY+ +
Sbjct: 154 NL--QISISTTVTTTSLARLKPPSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPYYFH 213

Query: 185 ASNPQQISLDYALFTSTTPVVVDGDLQYLNLFDAMVDSFYAALEKIDAGGVRIEISETGW 244
           AS+P+ + L+YA F +   VV DG L+Y NLFDA+ D+F  A+EK    G+ + +SETGW
Sbjct: 214 ASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSETGW 273

Query: 245 PTKGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNIKYDVVFFEMFNENLKTFGVEQNF 301
           P+ GN   T+     TY  NFVKHV SG GTPKRPN + D   FE +NEN K  G+ Q+F
Sbjct: 274 PSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIYQHF 333

The following BLAST results are available for this feature:

BLAST of Lsi04G023610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22317	4.3e-74	46.45	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
O49352	1.3e-70	46.50	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49353	7.1e-69	45.22	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q02439	4.2e-61	43.17	Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4...	[more]
A7PQW3	5.4e-61	42.31	Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120...	[more]

BLAST of Lsi04G023610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V7B6	1.1e-138	81.47	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3D504	1.1e-138	81.47	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5D3D518	2.6e-138	80.25	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S4DV56	2.6e-138	80.25	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV...	[more]
A0A0A0L3A5	1.1e-136	78.98	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3...	[more]

BLAST of Lsi04G023610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896614.1	6.2e-139	80.57	glucan endo-1,3-beta-glucosidase-like [Benincasa hispida]	[more]
KAA0064262.1	2.4e-138	81.47	putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa]	[more]
TYK18631.1	2.4e-138	81.47	putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa]	[more]
XP_016899853.1	5.3e-138	80.25	PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo]	[more]
TYK18632.1	5.3e-138	80.25	putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi04G023610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77780.1	9.8e-74	45.78	Glycosyl hydrolase superfamily protein	[more]
AT5G20330.1	9.2e-72	46.50	beta-1,3-glucanase 4	[more]
AT1G77790.1	4.5e-71	47.23	Glycosyl hydrolase superfamily protein	[more]
AT5G20340.1	5.0e-70	45.22	beta-1,3-glucanase 5	[more]
AT5G20560.1	1.4e-67	43.93	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 5..307 e-value: 2.3E-107 score: 360.6
None	No IPR available	PANTHER	PTHR32227:SF248	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 4..307
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 5..305 e-value: 3.5E-81 score: 273.2
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 4..307
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 5..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G023610.1	Lsi04G023610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds