Lsi04G023460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G023460
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMFS domain-containing protein
Locationchr04: 30634184 .. 30638051 (-)
RNA-Seq ExpressionLsi04G023460
SyntenyLsi04G023460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAACATGCAATCGTAGGAGGGACGGGATCCAATTGGCATATAAAGTTGGGTGCCAACTACTACAAATTGAAGCCTAAATTCGCGGGTGGCTCAGGGAACAGCAGAAAAGTCTAGGCGGCGGGCCGATATGAAGCCGGAGACGGTGACACTGATTCTGGTCAACCTCGCCGGAGTCATGGAGAGGGCCGACGAATCTCTCTTACCTGGTGTCTACAAAGAGGTCGGTGCCGCCTTGCACACCGATCCTACTGGTCTCGGCTCCTTGACTCTTTTCAGATCCATCGTTCAGGCCGCTTGCTATCCCTTGGCTGCTTATATGGCCGCCCGCCATAACCGAGCTCATGTGATTGCTCTCGGCGCTTTCCTCTGGGCCGCCGCTACGTTTCTCGTCGCCTTTTCCTCTACTTTCCTTCAGGTTCATCCAATTCTACTCTTGCTCCTGATTCTTTTTTCCCCTTTTCATTTCACTATTGTGTAGCCCTAGATAATGCTACATTCTTCCCCCTTGTTTTGATCTCGCCAATTTTGCTTTATTCTCTTCGCTTAATTGGGTGTTCGCTTTCCGGATATTATTGTGTACGTATAATAACAAATGGAAGTTTAGGGCTGTCGCACGAATCAATTGAAATAGGACGTATTTCTCTTCCTTGTTCTTGTATGAATAATCATTAAAAAAAAAATGGGAAAGTTGGAACACTAACCATTAAACCGTTCCAAAATGAGAATAGTAAAAATAACAAGCCATATCAATTAGTGTTTTACCATGTTAATTTTTCCATATCCACCTACTGCAGAAAATTAGTGAAACATTATGAAGTGGACTTCGTATGAAGATTTGGAACACTTTTTTTTTCTTTTGAAAGTTTGAAAGGATGAACATTGTTGTGTAGCCTTTGACAGTTTTAGTGAGGCGCAATCTTTGCTCATTTCTGGTTGGAAATGTTCTTTTCTCAGTAGATTGCACCATGCGTTCATCATGTGCAAAAATACATTGTTAATTACTTGAAAGCATATAGCAAGGAATAATGTGAGATCATGAAGGATCAGAATCGATAATTTACATGTCACTGAATCACCTGAAGTATTAAATCATTTACTACTTCATCTCTCATTCTGCCAACTCCCACCAAAACTTTGTGACAACGGTTAAGAACTGGTATATTAGGAAGTTAGTAATAAATTGGCGACTGAGAAACTGATTTTAACACCCTTGTGGTTTAATGGGATTGTTTCGCTGTTTGTTGATTCTGTTCACACTTATATGTTCTGTTGGAAGCTTGGGTATGAAGCCAGTTGCCTTGGGGAAATAAAGAAATAGATGTGAAAATTCCAGTTATAAAGATTTTGGATGATTTATTGGAAGAGGGAAAGCCTGCACAGATCTATAGACAATAAATTTTAATTTCCATTGGTGTTTAGAAATTTTGTTAAGTCAACAAAACTTGGGCAAGTTGATTTGTTAGAAGTTTTTAGTTATTAATATATTAATATGTGGCAGATTTACCTATGCTGGAATTAAATATTAGCTGATTATAAGTCATTCAGATTGTATCCAACAGGTCGCTGTTTCAAGAGCATTGAATGGTATTGGCCTCGCATTAGTTGCACCTGCCATCCAGTCCCTTGTAGCAGATTCGACAGATGACAATAACCGTGGTGTGGCTTTTGGATGGCTTCAAACAACAGGCAACCTAGGCTCCATTATTGGCGGGTTGTGTTCAATTGTAATAGCTCCAATAACATTTATGGGAATCCCCGGTTGGAGAATTGCATTTCATCTAGTTGGATTAATCAGTGTTGTTGTTGGCATTCTAGTCCGTTTCTTTGCACAGGATCCACATTTTTTGGATGACGGCATAAAAACTGGTAATCATGTCCCACCCCATAATTCTTTTTGGTCTGAAGTGAAGGTCTTGGTAAGGGAATCCAAATCAGTTATAAAGATTCCATCCTTTCAGATCATCGTAGCTCAGGGTGTCACAGGTTCATTTCCCTGGTCGGCCTTGTCATTTGCAACAATGTGGTTGGAGCTCAAGGGCTTCTCCCATGAGAAGACTGCTTTTCTGATGGGCATATTTGTGATTGGAAACTCTCTTGGAGGTTTGTTTGGAGGTAAAATGGGAGATATCCTCTCCACACGTTTCCCAAACTCTGGGAGGATTATTCTTGCCCAGATCAGCTCAGCATCAGGCGTTCCCCTAGCTGCAGTTCTTCTGCTCTTCTTGCCTGATGGCCCCTCTACAGCATTCATCCATGGACTAGTCTTGATCATTGTGGGGTTTTTCATTTCTTGGAATGCTCCAGCTACAAATAAGTAAGTGTCATATTTGCCGTCATCTATCTCAGTCTATGAGAAAAACTAGTGTTCAGGTGGGCTAATTACATAGAAATTGAAAAGGGAATATCTAGTTTTCCTGAAAATTGAATAATAAGCAATACATCTTTTGAATAATAGGTTTTCACCAAAACTAAAAAGAATCCTACGTGGTAGTTTTAATTTTGCTTCATAAGCCAACTAGTCTTGCCTAGAATCTGGAAGGACCAATGTGTTTTGACTTGGGAGATTACAAACACGACCTAGAGGATAATAAGCATCATTAGAGATGACTATATATTGATTACTTGTGAACTATACAAAGAAATGCACATCAAGAATAGAATGTTAATACAACCTTGAACGTTAAATGGATAGCAATCGAATTTGTAGGCGTGGTACATTACTCATACCTCATGTATGAAAGGAACAAAGATACGCTATTGACTTCGAATCATGATTTGATTTATTTTCTTGCAGTCCTATTTTTGCAGAGATAGTTCCTGAGAAATCGCGGACTAGTGTGTATGCTTTAGATCGGTCCTTCGAGTCTATTTTGTCATCATTTGCCCCTCCTGTTGTCGGAATTCTGGCTCAGCATGTCTATGGATATAAGCCAGTTCAGAAAGGATCTAGTGAATCTGAAGAGATTGCCACAGATAGGCAGAATGCAGCATCATTAGCCCGAGCATTGTACACAGCAATAGGAATTCCATTGGCTCTCTGTTGTTTAATTTACTCGTTTCTCTACTGCACCTATCCAAGAGATAGAGAACGAGCACGTATGGAAGTTTTAATTGAATCAGAGATGCAGCAAATAGAATCTAAAAGGTCACCTTCAGGTTCTGGATACTCTCAAGTTCATTTAGCAGGGTCGGACGAGCTATATGCTAGAGATAGAACAGTCATCGATATGGATTATGAGGATGATCTAGATTTTGATGATGATGAAAAGACGGCCCTTTATCGCCAGTTGACTTTTTCAAATTTTGTCAAACAATAGCCTTTTGAGGGGATGTACTTTGTTGTCTAGCTTTTTACTCACAGGGAGAGCAAAAAAGCGACTCCATTCCACGAATTGGGCTTGAATCTTGACGGTTAGGAAGTATTATAATTTTGAATCAAAACAGAAGATGAAATATTCTTTTAAGAATTACAATGGGAAGATATATTGGGTCCATAGCTCAGTAGAGCATTTGACTGCAATGGGTAGATATATTTCAGTTTATATTGTGACAGATTAAAAGGACTCGCATGAAGAACATTTGGCCAGTCGTCCTCTTGCAGAGTTTGTAGATTATCATACGTTCCTCTGCTATGTTTACGTGTATATGTTCAACCAAACTTGTCTCCTCATATTGGTGTAACATAGACACTATTCTCATTCGTTTTCATAGTTCCAAGGAGTCTAAACATTTGTTGTGCATTACTAAATTCTTATTTATACAATGTGCTTGGATGAGTACATAATAAGCTCTTTTCCAAGCTTTTCTTGTAGGTGTTTCTGATTCCTACTTTCTGGAGTAAAGGTTTTGCACATAGCATTAAATAAA

mRNA sequence

GGAACATGCAATCGTAGGAGGGACGGGATCCAATTGGCATATAAAGTTGGGTGCCAACTACTACAAATTGAAGCCTAAATTCGCGGGTGGCTCAGGGAACAGCAGAAAAGTCTAGGCGGCGGGCCGATATGAAGCCGGAGACGGTGACACTGATTCTGGTCAACCTCGCCGGAGTCATGGAGAGGGCCGACGAATCTCTCTTACCTGGTGTCTACAAAGAGGTCGGTGCCGCCTTGCACACCGATCCTACTGGTCTCGGCTCCTTGACTCTTTTCAGATCCATCGTTCAGGCCGCTTGCTATCCCTTGGCTGCTTATATGGCCGCCCGCCATAACCGAGCTCATGTGATTGCTCTCGGCGCTTTCCTCTGGGCCGCCGCTACGTTTCTCGTCGCCTTTTCCTCTACTTTCCTTCAGGTCGCTGTTTCAAGAGCATTGAATGGTATTGGCCTCGCATTAGTTGCACCTGCCATCCAGTCCCTTGTAGCAGATTCGACAGATGACAATAACCGTGGTGTGGCTTTTGGATGGCTTCAAACAACAGGCAACCTAGGCTCCATTATTGGCGGGTTGTGTTCAATTGTAATAGCTCCAATAACATTTATGGGAATCCCCGGTTGGAGAATTGCATTTCATCTAGTTGGATTAATCAGTGTTGTTGTTGGCATTCTAGTCCGTTTCTTTGCACAGGATCCACATTTTTTGGATGACGGCATAAAAACTGGTAATCATGTCCCACCCCATAATTCTTTTTGGTCTGAAGTGAAGGTCTTGGTAAGGGAATCCAAATCAGTTATAAAGATTCCATCCTTTCAGATCATCGTAGCTCAGGGTGTCACAGGTTCATTTCCCTGGTCGGCCTTGTCATTTGCAACAATGTGGTTGGAGCTCAAGGGCTTCTCCCATGAGAAGACTGCTTTTCTGATGGGCATATTTGTGATTGGAAACTCTCTTGGAGGTTTGTTTGGAGGTAAAATGGGAGATATCCTCTCCACACGTTTCCCAAACTCTGGGAGGATTATTCTTGCCCAGATCAGCTCAGCATCAGGCGTTCCCCTAGCTGCAGTTCTTCTGCTCTTCTTGCCTGATGGCCCCTCTACAGCATTCATCCATGGACTAGTCTTGATCATTGTGGGGTTTTTCATTTCTTGGAATGCTCCAGCTACAAATAATCCTATTTTTGCAGAGATAGTTCCTGAGAAATCGCGGACTAGTGTGTATGCTTTAGATCGGTCCTTCGAGTCTATTTTGTCATCATTTGCCCCTCCTGTTGTCGGAATTCTGGCTCAGCATGTCTATGGATATAAGCCAGTTCAGAAAGGATCTAGTGAATCTGAAGAGATTGCCACAGATAGGCAGAATGCAGCATCATTAGCCCGAGCATTGTACACAGCAATAGGAATTCCATTGGCTCTCTGTTGTTTAATTTACTCGTTTCTCTACTGCACCTATCCAAGAGATAGAGAACGAGCACGTATGGAAGTTTTAATTGAATCAGAGATGCAGCAAATAGAATCTAAAAGGTCACCTTCAGGTTCTGGATACTCTCAAGTTCATTTAGCAGGGTCGGACGAGCTATATGCTAGAGATAGAACAGTCATCGATATGGATTATGAGGATGATCTAGATTTTGATGATGATGAAAAGACGGCCCTTTATCGCCAGTTGACTTTTTCAAATTTTGTCAAACAATAGCCTTTTGAGGGGATGTACTTTGTTGTCTAGCTTTTTACTCACAGGGAGAGCAAAAAAGCGACTCCATTCCACGAATTGGGCTTGAATCTTGACGGTTAGGAAGTATTATAATTTTGAATCAAAACAGAAGATGAAATATTCTTTTAAGAATTACAATGGGAAGATATATTGGGTCCATAGCTCAGTAGAGCATTTGACTGCAATGGGTAGATATATTTCAGTTTATATTGTGACAGATTAAAAGGACTCGCATGAAGAACATTTGGCCAGTCGTCCTCTTGCAGAGTTTGTAGATTATCATACGTTCCTCTGCTATGTTTACGTGTATATGTTCAACCAAACTTGTCTCCTCATATTGGTGTAACATAGACACTATTCTCATTCGTTTTCATAGTTCCAAGGAGTCTAAACATTTGTTGTGCATTACTAAATTCTTATTTATACAATGTGCTTGGATGAGTACATAATAAGCTCTTTTCCAAGCTTTTCTTGTAGGTGTTTCTGATTCCTACTTTCTGGAGTAAAGGTTTTGCACATAGCATTAAATAAA

Coding sequence (CDS)

ATGAAGCCGGAGACGGTGACACTGATTCTGGTCAACCTCGCCGGAGTCATGGAGAGGGCCGACGAATCTCTCTTACCTGGTGTCTACAAAGAGGTCGGTGCCGCCTTGCACACCGATCCTACTGGTCTCGGCTCCTTGACTCTTTTCAGATCCATCGTTCAGGCCGCTTGCTATCCCTTGGCTGCTTATATGGCCGCCCGCCATAACCGAGCTCATGTGATTGCTCTCGGCGCTTTCCTCTGGGCCGCCGCTACGTTTCTCGTCGCCTTTTCCTCTACTTTCCTTCAGGTCGCTGTTTCAAGAGCATTGAATGGTATTGGCCTCGCATTAGTTGCACCTGCCATCCAGTCCCTTGTAGCAGATTCGACAGATGACAATAACCGTGGTGTGGCTTTTGGATGGCTTCAAACAACAGGCAACCTAGGCTCCATTATTGGCGGGTTGTGTTCAATTGTAATAGCTCCAATAACATTTATGGGAATCCCCGGTTGGAGAATTGCATTTCATCTAGTTGGATTAATCAGTGTTGTTGTTGGCATTCTAGTCCGTTTCTTTGCACAGGATCCACATTTTTTGGATGACGGCATAAAAACTGGTAATCATGTCCCACCCCATAATTCTTTTTGGTCTGAAGTGAAGGTCTTGGTAAGGGAATCCAAATCAGTTATAAAGATTCCATCCTTTCAGATCATCGTAGCTCAGGGTGTCACAGGTTCATTTCCCTGGTCGGCCTTGTCATTTGCAACAATGTGGTTGGAGCTCAAGGGCTTCTCCCATGAGAAGACTGCTTTTCTGATGGGCATATTTGTGATTGGAAACTCTCTTGGAGGTTTGTTTGGAGGTAAAATGGGAGATATCCTCTCCACACGTTTCCCAAACTCTGGGAGGATTATTCTTGCCCAGATCAGCTCAGCATCAGGCGTTCCCCTAGCTGCAGTTCTTCTGCTCTTCTTGCCTGATGGCCCCTCTACAGCATTCATCCATGGACTAGTCTTGATCATTGTGGGGTTTTTCATTTCTTGGAATGCTCCAGCTACAAATAATCCTATTTTTGCAGAGATAGTTCCTGAGAAATCGCGGACTAGTGTGTATGCTTTAGATCGGTCCTTCGAGTCTATTTTGTCATCATTTGCCCCTCCTGTTGTCGGAATTCTGGCTCAGCATGTCTATGGATATAAGCCAGTTCAGAAAGGATCTAGTGAATCTGAAGAGATTGCCACAGATAGGCAGAATGCAGCATCATTAGCCCGAGCATTGTACACAGCAATAGGAATTCCATTGGCTCTCTGTTGTTTAATTTACTCGTTTCTCTACTGCACCTATCCAAGAGATAGAGAACGAGCACGTATGGAAGTTTTAATTGAATCAGAGATGCAGCAAATAGAATCTAAAAGGTCACCTTCAGGTTCTGGATACTCTCAAGTTCATTTAGCAGGGTCGGACGAGCTATATGCTAGAGATAGAACAGTCATCGATATGGATTATGAGGATGATCTAGATTTTGATGATGATGAAAAGACGGCCCTTTATCGCCAGTTGACTTTTTCAAATTTTGTCAAACAATAG

Protein sequence

MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVADSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSFPWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILAQISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSRTSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALYTAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGSDELYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ
Homology
BLAST of Lsi04G023460 vs. ExPASy TrEMBL
Match: A0A0A0LE77 (MFS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G895860 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.5e-278
Identity = 493/521 (94.63%), Postives = 509/521 (97.70%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL
Sbjct: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWA+ATFLVAFSSTFLQVA+SR LNGIGLALVAPAIQSLVA
Sbjct: 61  AAYLAVRHNRAHVIALGAFLWASATFLVAFSSTFLQVAISRGLNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDD+NRG+AFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISV+VGI
Sbjct: 121 DSTDDSNRGLAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVIVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVR FAQDPHFLDDGIK GN VPP +SFWSEVKVL RE+KSV+KIPSFQIIVAQGVTGSF
Sbjct: 181 LVRLFAQDPHFLDDGIKIGNDVPPQSSFWSEVKVLAREAKSVMKIPSFQIIVAQGVTGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFATMWLELKGFSH+KTAFLMG+FVIGNSLGGLFGGKMGDILSTRFPNSGRIILA
Sbjct: 241 PWSALSFATMWLELKGFSHQKTAFLMGMFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISS SG+PLAAVLLLFLPDGPSTA IHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSGSGIPLAAVLLLFLPDGPSTAVIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDR+NAASLARALY
Sbjct: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRENAASLARALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IYSFLYCTYPRDRERARMEVLIESEMQQIES+RSPSG+GYSQVHLAGS
Sbjct: 421 TAIGIPLALCCFIYSFLYCTYPRDRERARMEVLIESEMQQIESERSPSGAGYSQVHLAGS 480

Query: 481 DELYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ 522
           D+LY  DRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ
Sbjct: 481 DDLYTTDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ 521

BLAST of Lsi04G023460 vs. ExPASy TrEMBL
Match: A0A1S3CRQ7 (high affinity nitrate transporter 2.5 OS=Cucumis melo OX=3656 GN=LOC103503585 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.5e-278
Identity = 492/521 (94.43%), Postives = 510/521 (97.89%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL
Sbjct: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWA+ATFLVAFSSTFLQVA+SR LNGIGLALVAPAIQSLVA
Sbjct: 61  AAYLAVRHNRAHVIALGAFLWASATFLVAFSSTFLQVAISRGLNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDD+NRG+AFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI
Sbjct: 121 DSTDDSNRGLAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVR FAQDPHFLDDGIK GN+VPP +SFWSEVKVL RE+KSV+KIPSFQIIVAQGVTGSF
Sbjct: 181 LVRLFAQDPHFLDDGIKMGNNVPPRSSFWSEVKVLAREAKSVMKIPSFQIIVAQGVTGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFATMWLELKGFSHE TAFLMG+FVIGNSLGGLFGGKMGD+LSTRFPNSGRIILA
Sbjct: 241 PWSALSFATMWLELKGFSHETTAFLMGMFVIGNSLGGLFGGKMGDVLSTRFPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISS SG+PLAAVLLLFLPDGPSTA IHGLVLI+VGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSGSGIPLAAVLLLFLPDGPSTAVIHGLVLIVVGFFISWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDR+NAASLARALY
Sbjct: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRENAASLARALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IYSFLYCTYPRDRERARMEVLIESEMQQIES+RSPSG+GYSQVHL GS
Sbjct: 421 TAIGIPLALCCFIYSFLYCTYPRDRERARMEVLIESEMQQIESERSPSGAGYSQVHLGGS 480

Query: 481 DELYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ 522
           D+LYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFV+Q
Sbjct: 481 DDLYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVQQ 521

BLAST of Lsi04G023460 vs. ExPASy TrEMBL
Match: A0A5D3E4Z8 (High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004880 PE=4 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.3e-269
Identity = 476/505 (94.26%), Postives = 494/505 (97.82%), Query Frame = 0

Query: 17  MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAYMAARHNRAHVIAL 76
           MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAY+A RHNRAHVIAL
Sbjct: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAYLAVRHNRAHVIAL 60

Query: 77  GAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVADSTDDNNRGVAFGWLQ 136
           GAFLWA+ATFLVAFSSTFLQVA+SR LNGIGLALVAPAIQSLVADSTDD+NRG+AFGWLQ
Sbjct: 61  GAFLWASATFLVAFSSTFLQVAISRGLNGIGLALVAPAIQSLVADSTDDSNRGLAFGWLQ 120

Query: 137 TTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVRFFAQDPHFLDDGI 196
           TTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVR FAQDPHFLDDGI
Sbjct: 121 TTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVRLFAQDPHFLDDGI 180

Query: 197 KTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSFPWSALSFATMWLELKG 256
           K GN+VPP +SFWSEVKVL RE+KSV+KIPSFQIIVAQGVTGSFPWSALSFATMWLELKG
Sbjct: 181 KMGNNVPPRSSFWSEVKVLAREAKSVMKIPSFQIIVAQGVTGSFPWSALSFATMWLELKG 240

Query: 257 FSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILAQISSASGVPLAAVLLL 316
           FSHE TAFLMG+FVIGNSLGGLFGGKMGD+LSTRFPNSGRIILAQISS SG+PLAAVLLL
Sbjct: 241 FSHETTAFLMGMFVIGNSLGGLFGGKMGDVLSTRFPNSGRIILAQISSGSGIPLAAVLLL 300

Query: 317 FLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSRTSVYALDRSFESILSS 376
           FLPDGPSTA IHGLVLI+VGFFISWNAPATNNPIFAEIVPEKSRTSVYALDRSFESILSS
Sbjct: 301 FLPDGPSTAVIHGLVLIVVGFFISWNAPATNNPIFAEIVPEKSRTSVYALDRSFESILSS 360

Query: 377 FAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALYTAIGIPLALCCLIYSF 436
           FAPPVVGILAQHVYGYKPVQKGSSESEEIATDR+NAASLARALYTAIGIPLALCC IYSF
Sbjct: 361 FAPPVVGILAQHVYGYKPVQKGSSESEEIATDRENAASLARALYTAIGIPLALCCFIYSF 420

Query: 437 LYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGSDELYARDRTVIDMDYE 496
           LYCTYPRDRERARMEVLIESEMQQIES+RSPSG+GYSQVHL GSD+LYARDRTVIDMDYE
Sbjct: 421 LYCTYPRDRERARMEVLIESEMQQIESERSPSGAGYSQVHLGGSDDLYARDRTVIDMDYE 480

Query: 497 DDLDFDDDEKTALYRQLTFSNFVKQ 522
           DDLDFDDDEKTALYRQLTFSNFV+Q
Sbjct: 481 DDLDFDDDEKTALYRQLTFSNFVQQ 505

BLAST of Lsi04G023460 vs. ExPASy TrEMBL
Match: A0A6J1FJL7 (uncharacterized protein LOC111445948 OS=Cucurbita moschata OX=3662 GN=LOC111445948 PE=4 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.6e-267
Identity = 487/526 (92.59%), Postives = 499/526 (94.87%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQA+CYPL
Sbjct: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQASCYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA
Sbjct: 61  AAYLAVRHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIV+AP TFMGIPGWRIAFH+VGLISVVVGI
Sbjct: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVLAPRTFMGIPGWRIAFHIVGLISVVVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVRFFA DPHF DDGIKTGNH PP NSFWSEVK LVRESKSVIKIPSFQIIVAQG+TGSF
Sbjct: 181 LVRFFAHDPHFSDDGIKTGNHAPPSNSFWSEVKDLVRESKSVIKIPSFQIIVAQGITGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFATMWLELKGFSHE TAFLMG+FVIGNSLGGLFGGKMGDILSTRFPNSGRIILA
Sbjct: 241 PWSALSFATMWLELKGFSHETTAFLMGMFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISSASG+PLAAVLLL LPDGPSTA +HGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSASGIPLAAVLLLLLPDGPSTAVVHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDR NAASLA ALY
Sbjct: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRGNAASLAWALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IY+FLYCTYPRDRERARMEVLIESEMQQI+S+R  SG GYSQV L  S
Sbjct: 421 TAIGIPLALCCFIYTFLYCTYPRDRERARMEVLIESEMQQIQSERPTSGPGYSQVQLEES 480

Query: 481 DELYARDRTVID-MDY--EDDLDF---DDDEKTALYRQLTFSNFVK 521
           D+LYARDR VID MDY  EDDLDF   DDDEKTALYRQLTFS FVK
Sbjct: 481 DKLYARDRKVIDMMDYEEEDDLDFDDDDDDEKTALYRQLTFSTFVK 526

BLAST of Lsi04G023460 vs. ExPASy TrEMBL
Match: A0A6J1CP31 (uncharacterized protein LOC111012796 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012796 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.1e-267
Identity = 478/522 (91.57%), Postives = 496/522 (95.02%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MK ETVTL+LVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTL RSIVQA+CYPL
Sbjct: 1   MKAETVTLVLVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLLRSIVQASCYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA
Sbjct: 61  AAYLAVRHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDD+NRGVAFGWLQ TGNLGSIIGGLCSI+IAP+TFMGIPGWRIAFHLVGLISV VGI
Sbjct: 121 DSTDDSNRGVAFGWLQLTGNLGSIIGGLCSIIIAPMTFMGIPGWRIAFHLVGLISVFVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVRFFA DPHF DDG KT NHVPP+NSFWSEVK LVRESKSV+KIPSFQIIVAQG+TGSF
Sbjct: 181 LVRFFAHDPHFSDDGTKTSNHVPPNNSFWSEVKDLVRESKSVMKIPSFQIIVAQGITGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFA MWLELKGFSHEKTAFLMG+FV+GNSLGGLFGGKMGDILSTR PNSGRIILA
Sbjct: 241 PWSALSFAAMWLELKGFSHEKTAFLMGMFVVGNSLGGLFGGKMGDILSTRLPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISSASGVPLAAVLLLFLPD PSTAF+HGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSASGVPLAAVLLLFLPDSPSTAFVHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVG+LAQHVYGYKPVQKGSSESEEIATDR NAASLARALY
Sbjct: 361 TSVYALDRSFESILSSFAPPVVGLLAQHVYGYKPVQKGSSESEEIATDRGNAASLARALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IYSFLYCTYPRD+ERARME LIESEMQQIES+   SG+GYSQVH A S
Sbjct: 421 TAIGIPLALCCFIYSFLYCTYPRDKERARMEALIESEMQQIESESPSSGAGYSQVHFAES 480

Query: 481 DELYARDRTVIDMDY--EDDLDFDDDEKTALYRQLTFSNFVK 521
           DELYARDRTVID+DY  EDDL FDDDEK AL RQLTF+NFVK
Sbjct: 481 DELYARDRTVIDVDYEEEDDLHFDDDEKAALSRQLTFANFVK 522

BLAST of Lsi04G023460 vs. NCBI nr
Match: XP_038898006.1 (Na(+), Li(+), K(+)/H(+) antiporter [Benincasa hispida])

HSP 1 Score: 969.5 bits (2505), Expect = 1.1e-278
Identity = 497/521 (95.39%), Postives = 511/521 (98.08%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL
Sbjct: 37  MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 96

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA
Sbjct: 97  AAYLAVRHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 156

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDDNNRGVAFGWLQTTGNLGSIIGGL SIVIAPITFMGIPGWRIAFHLVGLISVVVGI
Sbjct: 157 DSTDDNNRGVAFGWLQTTGNLGSIIGGLFSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 216

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVR FA DPHFLDDGIKTGNHVP H+SFWSEVKVLVRESK+V+KIPSFQIIVAQGVTGSF
Sbjct: 217 LVRLFAHDPHFLDDGIKTGNHVPAHSSFWSEVKVLVRESKAVMKIPSFQIIVAQGVTGSF 276

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFATMWLELKGFSH+ TAFLMG+FVIGNSLGGLFGGKMGDILSTRFPNSGRIIL+
Sbjct: 277 PWSALSFATMWLELKGFSHDMTAFLMGMFVIGNSLGGLFGGKMGDILSTRFPNSGRIILS 336

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISS SG+PLAAVLLLFLPDGPSTA IHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 337 QISSGSGIPLAAVLLLFLPDGPSTAVIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 396

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGS+ESEEIATDR+NAASLARALY
Sbjct: 397 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSTESEEIATDRENAASLARALY 456

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IYSFLYCTYPRDRERARMEVLIESEMQQIES+RSPSG+GYSQVHLAGS
Sbjct: 457 TAIGIPLALCCFIYSFLYCTYPRDRERARMEVLIESEMQQIESERSPSGAGYSQVHLAGS 516

Query: 481 DELYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ 522
           DE+YA DRTVIDMDYED+LDFDDDEKTALYRQLTFSNFVKQ
Sbjct: 517 DEVYATDRTVIDMDYEDNLDFDDDEKTALYRQLTFSNFVKQ 557

BLAST of Lsi04G023460 vs. NCBI nr
Match: XP_004136402.1 (uncharacterized protein LOC101220496 [Cucumis sativus])

HSP 1 Score: 966.8 bits (2498), Expect = 7.3e-278
Identity = 493/521 (94.63%), Postives = 509/521 (97.70%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL
Sbjct: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWA+ATFLVAFSSTFLQVA+SR LNGIGLALVAPAIQSLVA
Sbjct: 61  AAYLAVRHNRAHVIALGAFLWASATFLVAFSSTFLQVAISRGLNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDD+NRG+AFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISV+VGI
Sbjct: 121 DSTDDSNRGLAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVIVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVR FAQDPHFLDDGIK GN VPP +SFWSEVKVL RE+KSV+KIPSFQIIVAQGVTGSF
Sbjct: 181 LVRLFAQDPHFLDDGIKIGNDVPPQSSFWSEVKVLAREAKSVMKIPSFQIIVAQGVTGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFATMWLELKGFSH+KTAFLMG+FVIGNSLGGLFGGKMGDILSTRFPNSGRIILA
Sbjct: 241 PWSALSFATMWLELKGFSHQKTAFLMGMFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISS SG+PLAAVLLLFLPDGPSTA IHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSGSGIPLAAVLLLFLPDGPSTAVIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDR+NAASLARALY
Sbjct: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRENAASLARALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IYSFLYCTYPRDRERARMEVLIESEMQQIES+RSPSG+GYSQVHLAGS
Sbjct: 421 TAIGIPLALCCFIYSFLYCTYPRDRERARMEVLIESEMQQIESERSPSGAGYSQVHLAGS 480

Query: 481 DELYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ 522
           D+LY  DRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ
Sbjct: 481 DDLYTTDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ 521

BLAST of Lsi04G023460 vs. NCBI nr
Match: XP_008466033.1 (PREDICTED: high affinity nitrate transporter 2.5 [Cucumis melo])

HSP 1 Score: 966.8 bits (2498), Expect = 7.3e-278
Identity = 492/521 (94.43%), Postives = 510/521 (97.89%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL
Sbjct: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWA+ATFLVAFSSTFLQVA+SR LNGIGLALVAPAIQSLVA
Sbjct: 61  AAYLAVRHNRAHVIALGAFLWASATFLVAFSSTFLQVAISRGLNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDD+NRG+AFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI
Sbjct: 121 DSTDDSNRGLAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVR FAQDPHFLDDGIK GN+VPP +SFWSEVKVL RE+KSV+KIPSFQIIVAQGVTGSF
Sbjct: 181 LVRLFAQDPHFLDDGIKMGNNVPPRSSFWSEVKVLAREAKSVMKIPSFQIIVAQGVTGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFATMWLELKGFSHE TAFLMG+FVIGNSLGGLFGGKMGD+LSTRFPNSGRIILA
Sbjct: 241 PWSALSFATMWLELKGFSHETTAFLMGMFVIGNSLGGLFGGKMGDVLSTRFPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISS SG+PLAAVLLLFLPDGPSTA IHGLVLI+VGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSGSGIPLAAVLLLFLPDGPSTAVIHGLVLIVVGFFISWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDR+NAASLARALY
Sbjct: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRENAASLARALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IYSFLYCTYPRDRERARMEVLIESEMQQIES+RSPSG+GYSQVHL GS
Sbjct: 421 TAIGIPLALCCFIYSFLYCTYPRDRERARMEVLIESEMQQIESERSPSGAGYSQVHLGGS 480

Query: 481 DELYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVKQ 522
           D+LYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFV+Q
Sbjct: 481 DDLYARDRTVIDMDYEDDLDFDDDEKTALYRQLTFSNFVQQ 521

BLAST of Lsi04G023460 vs. NCBI nr
Match: KAA0038604.1 (high affinity nitrate transporter 2.5 [Cucumis melo var. makuwa] >TYK31203.1 high affinity nitrate transporter 2.5 [Cucumis melo var. makuwa])

HSP 1 Score: 938.3 bits (2424), Expect = 2.8e-269
Identity = 476/505 (94.26%), Postives = 494/505 (97.82%), Query Frame = 0

Query: 17  MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAYMAARHNRAHVIAL 76
           MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAY+A RHNRAHVIAL
Sbjct: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAYLAVRHNRAHVIAL 60

Query: 77  GAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVADSTDDNNRGVAFGWLQ 136
           GAFLWA+ATFLVAFSSTFLQVA+SR LNGIGLALVAPAIQSLVADSTDD+NRG+AFGWLQ
Sbjct: 61  GAFLWASATFLVAFSSTFLQVAISRGLNGIGLALVAPAIQSLVADSTDDSNRGLAFGWLQ 120

Query: 137 TTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVRFFAQDPHFLDDGI 196
           TTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVR FAQDPHFLDDGI
Sbjct: 121 TTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVRLFAQDPHFLDDGI 180

Query: 197 KTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSFPWSALSFATMWLELKG 256
           K GN+VPP +SFWSEVKVL RE+KSV+KIPSFQIIVAQGVTGSFPWSALSFATMWLELKG
Sbjct: 181 KMGNNVPPRSSFWSEVKVLAREAKSVMKIPSFQIIVAQGVTGSFPWSALSFATMWLELKG 240

Query: 257 FSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILAQISSASGVPLAAVLLL 316
           FSHE TAFLMG+FVIGNSLGGLFGGKMGD+LSTRFPNSGRIILAQISS SG+PLAAVLLL
Sbjct: 241 FSHETTAFLMGMFVIGNSLGGLFGGKMGDVLSTRFPNSGRIILAQISSGSGIPLAAVLLL 300

Query: 317 FLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSRTSVYALDRSFESILSS 376
           FLPDGPSTA IHGLVLI+VGFFISWNAPATNNPIFAEIVPEKSRTSVYALDRSFESILSS
Sbjct: 301 FLPDGPSTAVIHGLVLIVVGFFISWNAPATNNPIFAEIVPEKSRTSVYALDRSFESILSS 360

Query: 377 FAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALYTAIGIPLALCCLIYSF 436
           FAPPVVGILAQHVYGYKPVQKGSSESEEIATDR+NAASLARALYTAIGIPLALCC IYSF
Sbjct: 361 FAPPVVGILAQHVYGYKPVQKGSSESEEIATDRENAASLARALYTAIGIPLALCCFIYSF 420

Query: 437 LYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGSDELYARDRTVIDMDYE 496
           LYCTYPRDRERARMEVLIESEMQQIES+RSPSG+GYSQVHL GSD+LYARDRTVIDMDYE
Sbjct: 421 LYCTYPRDRERARMEVLIESEMQQIESERSPSGAGYSQVHLGGSDDLYARDRTVIDMDYE 480

Query: 497 DDLDFDDDEKTALYRQLTFSNFVKQ 522
           DDLDFDDDEKTALYRQLTFSNFV+Q
Sbjct: 481 DDLDFDDDEKTALYRQLTFSNFVQQ 505

BLAST of Lsi04G023460 vs. NCBI nr
Match: KAG6608079.1 (Protein spinster, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 931.4 bits (2406), Expect = 3.4e-267
Identity = 487/526 (92.59%), Postives = 499/526 (94.87%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQA+CYPL
Sbjct: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQASCYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+A RHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA
Sbjct: 61  AAYLAVRHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIV+AP TFMGIPGWRIAFH+VGLISVVVGI
Sbjct: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVLAPRTFMGIPGWRIAFHIVGLISVVVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVRFFA DPHF DDGIKTGNH PP NSFWSEVK LVRESKSVIKIPSFQIIVAQG+TGSF
Sbjct: 181 LVRFFAHDPHFSDDGIKTGNHAPPSNSFWSEVKDLVRESKSVIKIPSFQIIVAQGITGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFATMWLELKGFSHE TAFLMG+FVIGNSLGGLFGGKMGDILSTRFPNSGRIILA
Sbjct: 241 PWSALSFATMWLELKGFSHETTAFLMGMFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISSASG+PLAAVLLL LPDGPSTA +HGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSASGIPLAAVLLLLLPDGPSTAVVHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDR NAASLA ALY
Sbjct: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRGNAASLAWALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQVHLAGS 480
           TAIGIPLALCC IY+FLYCTYPRDRERARMEVLIESEMQQI+S+R  SG GYSQV L  S
Sbjct: 421 TAIGIPLALCCFIYTFLYCTYPRDRERARMEVLIESEMQQIQSERPTSGPGYSQVQLEES 480

Query: 481 DELYARDRTVID-MDY--EDDLDFD---DDEKTALYRQLTFSNFVK 521
           D+LYARDR VID MDY  EDDLDFD   DDEKTALYRQLTFS FVK
Sbjct: 481 DKLYARDRKVIDMMDYEEEDDLDFDDDNDDEKTALYRQLTFSTFVK 526

BLAST of Lsi04G023460 vs. TAIR 10
Match: AT1G78130.1 (Major facilitator superfamily protein )

HSP 1 Score: 738.8 bits (1906), Expect = 3.0e-213
Identity = 373/474 (78.69%), Postives = 419/474 (88.40%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MK ET+TL+LVNLAG+MERADESLLPGVYKEVG ALHTDPTGLGSLTL RS+VQAACYPL
Sbjct: 1   MKAETMTLLLVNLAGIMERADESLLPGVYKEVGLALHTDPTGLGSLTLLRSMVQAACYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAYMA RHNRAHVIALGAFLW+AATFLVAFSSTF QVAVSRALNGIGLALVAPAIQSLVA
Sbjct: 61  AAYMAIRHNRAHVIALGAFLWSAATFLVAFSSTFFQVAVSRALNGIGLALVAPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDD NRG AFGWLQ T N+GSI+GGLCS++IAP+TFMGIPGWR+AFH+VG+ISV+VG+
Sbjct: 121 DSTDDANRGTAFGWLQLTANIGSILGGLCSVLIAPLTFMGIPGWRVAFHIVGVISVIVGV 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVR FA DPHF+ DG+   N       F +EVK LVRE+ +VIKI SFQIIVAQGVTGSF
Sbjct: 181 LVRVFANDPHFVKDGVDVSNQPGSRKPFCTEVKDLVREADTVIKIRSFQIIVAQGVTGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSALSFA MWLEL GFSH KTAFLMG+FV  +SLGGLFGGKMGD LSTR PNSGRIILA
Sbjct: 241 PWSALSFAPMWLELIGFSHGKTAFLMGLFVAASSLGGLFGGKMGDFLSTRLPNSGRIILA 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           QISSAS +PLAA+LLL LPD PSTA IHGL+L+++G F+SWNAPATNNPIFAEIVPEKSR
Sbjct: 301 QISSASAIPLAAILLLVLPDDPSTAAIHGLILVLLGLFVSWNAPATNNPIFAEIVPEKSR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TSVYALD+SFESILSSFAPP+VGILAQHVYGYKP+ +GSS S EIATDR+NAASLA+ALY
Sbjct: 361 TSVYALDKSFESILSSFAPPIVGILAQHVYGYKPIPEGSSRSTEIATDRENAASLAKALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESKRSPSGSGYSQ 475
           T+IG+P+A CC IYSFLY +YP DR+RARME  I+SEM+++  + S     +SQ
Sbjct: 421 TSIGLPMAACCFIYSFLYRSYPLDRDRARMEAFIDSEMRELLPESSNRDIEFSQ 474

BLAST of Lsi04G023460 vs. TAIR 10
Match: AT5G10190.1 (Major facilitator superfamily protein )

HSP 1 Score: 652.5 bits (1682), Expect = 2.8e-187
Identity = 328/464 (70.69%), Postives = 392/464 (84.48%), Query Frame = 0

Query: 1   MKPETVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPL 60
           MK ET+TL+LV LAG+MERADESLLPGVYKEVG ALH DPT LG+LTLFRSIVQ++CYPL
Sbjct: 1   MKSETLTLVLVYLAGIMERADESLLPGVYKEVGDALHVDPTALGTLTLFRSIVQSSCYPL 60

Query: 61  AAYMAARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVA 120
           AAY+++RHNRAHVIALGAFLWA ATFLVA S+TF QVAVSR LNGIGLA+V PAIQSLVA
Sbjct: 61  AAYLSSRHNRAHVIALGAFLWATATFLVAVSTTFFQVAVSRGLNGIGLAIVTPAIQSLVA 120

Query: 121 DSTDDNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGI 180
           DSTDD NRG+AFGWL  T N+GSI+G +CSI+ A  +F G+ GWRIAF LV ++SV+VGI
Sbjct: 121 DSTDDYNRGMAFGWLGFTSNIGSILGYVCSILFASKSFNGVAGWRIAFLLVAVVSVIVGI 180

Query: 181 LVRFFAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSF 240
           LVR FA DPH+ D   K   HV     FWS+++ L++E+K VIKIPSFQI VAQGV+GSF
Sbjct: 181 LVRLFATDPHYSDR--KITKHV-KDKPFWSDIRDLLKEAKMVIKIPSFQIFVAQGVSGSF 240

Query: 241 PWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILA 300
           PWSAL+FA +WLEL GFSH+ TA L+ +F I  SLGGLFGG MGD L+ +FPN GRI L+
Sbjct: 241 PWSALAFAPLWLELIGFSHKTTAVLVTLFTISCSLGGLFGGYMGDTLAKKFPNGGRIFLS 300

Query: 301 QISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSR 360
           Q+SS S +PLAA+LL+ LPD PSTAF HGLVL+I+G  ISWN  ATN PIFAEIVPE++R
Sbjct: 301 QVSSGSAIPLAAILLIGLPDDPSTAFSHGLVLVIMGLCISWNGAATNGPIFAEIVPERAR 360

Query: 361 TSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDRQNAASLARALY 420
           TS+YALDRSFESIL+SFAPP+VG+LAQ++YGYKP+ +GS+ S +I TDR NAASLA+ALY
Sbjct: 361 TSIYALDRSFESILASFAPPIVGMLAQNIYGYKPIPEGSTSSVKIDTDRANAASLAKALY 420

Query: 421 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEMQQIESK 465
           T+IGIP+ +CC IYSFLYCTYPRDR+RA+M+ LIESEMQQ+  +
Sbjct: 421 TSIGIPMVICCTIYSFLYCTYPRDRDRAKMQALIESEMQQLNEE 461

BLAST of Lsi04G023460 vs. TAIR 10
Match: AT4G36790.1 (Major facilitator superfamily protein )

HSP 1 Score: 375.6 bits (963), Expect = 6.7e-104
Identity = 203/458 (44.32%), Postives = 288/458 (62.88%), Query Frame = 0

Query: 5   TVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAYM 64
           +++LIL+NLA +MERADE+LLP VYKEV  A +  P+ LG LT  R+ VQ    PLA  +
Sbjct: 42  SISLILINLAAIMERADENLLPSVYKEVSEAFNAGPSDLGYLTFVRNFVQGLASPLAGVL 101

Query: 65  AARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVADSTD 124
              ++R  V+A+G F WA +T  V  SS F+QVA+ RA+NG GLA+V PA+QS +ADS  
Sbjct: 102 VITYDRPIVLAIGTFCWALSTAAVGASSYFIQVALWRAVNGFGLAIVIPALQSFIADSYK 161

Query: 125 DNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVRF 184
           D  RG  FG L   G +G I GG+ + V+A   F GIPGWR AF ++  +S V+G+LV  
Sbjct: 162 DGARGAGFGMLNLIGTIGGIGGGVVATVMAGSEFWGIPGWRCAFIMMAALSAVIGLLVFL 221

Query: 185 FAQDPHFLDDGIKTGNHVPPHNSFWSEVKVLVRESKSVIKIPSFQIIVAQGVTGSFPWSA 244
           F  DP    +  +   H    NS W++    +  +KSV+K+ +FQIIVAQG+ GSFPW+A
Sbjct: 222 FVVDPRKNIEREELMAHKMNSNSVWND---SLAAAKSVVKVSTFQIIVAQGIIGSFPWTA 281

Query: 245 LSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILSTRFPNSGRIILAQISS 304
           + F TMW EL GF H +TA L+G+F  G ++G L GG + D +S  +PNSGR++ AQ S+
Sbjct: 282 MVFFTMWFELIGFDHNQTAALLGVFATGGAIGTLMGGIIADKMSRIYPNSGRVMCAQFSA 341

Query: 305 ASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNPIFAEIVPEKSRTSVY 364
             G+P + +LL  +P   S+  I  + L ++G  I+W   A N P+FAE+VP + RT +Y
Sbjct: 342 FMGIPFSIILLKVIPQSTSSYSIFSITLFLMGLTITWCGSAVNAPMFAEVVPPRHRTMIY 401

Query: 365 ALDRSFESILSSFAPPVVGILAQHVYGYK----PVQKGSSESEEIATDRQNAASLARALY 424
           A DR+FE   SSFA P+VGIL++ ++GY        KGSS  E        A +L++ L 
Sbjct: 402 AFDRAFEGSFSSFAAPLVGILSEKLFGYDSRGIDPLKGSSVRE--------ADALSKGLL 461

Query: 425 TAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEM 459
           + + +P  LCCL Y+ L+  + +DRE A++    E+EM
Sbjct: 462 SMMAVPFGLCCLCYTPLHFVFQKDRENAKIASSKETEM 488

BLAST of Lsi04G023460 vs. TAIR 10
Match: AT2G18590.1 (Major facilitator superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 2.8e-86
Identity = 184/469 (39.23%), Postives = 264/469 (56.29%), Query Frame = 0

Query: 5   TVTLILVNLAGVMERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSIVQAACYPLAAYM 64
           +++LI++NLA +M+RADE L+P   KE+  A H   + +G L+  R+IVQ    PLA   
Sbjct: 12  SISLIIINLATMMQRADEKLIPSTAKELKEAFHAKLSDIGLLSFIRNIVQGLASPLAGLF 71

Query: 65  AARHNRAHVIALGAFLWAAATFLVAFSSTFLQVAVSRALNGIGLALVAPAIQSLVADSTD 124
           A  ++R  V A G+F W ++T     S  F+QV +  A NG+G A+V P +QS++ADS  
Sbjct: 72  AISYDRPTVFAFGSFFWVSSTVATGVSRYFIQVTLGVAFNGVGHAIVYPVLQSIIADSFK 131

Query: 125 DNNRGVAFGWLQTTGNLGSIIGGLCSIVIAPITFMGIPGWRIAFHLVGLISVVVGILVRF 184
           +++RG  FG     G +G I G +   V+A   F GI GWR AF L   +S +VGILV F
Sbjct: 132 ESSRGFGFGLWNLIGTVGGIGGTVVPTVMAGHDFFGISGWRCAFILSATLSTIVGILVFF 191

Query: 185 FAQDP----------HFLDDGIKTGNH-----VPPHNSFWSEVKVLVRESKSVIKIPSFQ 244
           F  DP          H  D   +  N+       P +S W E  V +   K V K+ +FQ
Sbjct: 192 FVSDPREKKTSSVIVHHDDQHERDENNGGTMMESPSSSVWKESWVAI---KDVTKLRTFQ 251

Query: 245 IIVAQGVTGSFPWSALSFATMWLELKGFSHEKTAFLMGIFVIGNSLGGLFGGKMGDILST 304
           IIV QG+ GS PW+A+ F TMW EL GF H + A L GIF  G ++G L GG + D +S 
Sbjct: 252 IIVLQGIVGSVPWNAMLFWTMWFELIGFDHNQAALLNGIFATGQAIGSLVGGIIADKMSR 311

Query: 305 RFPNSGRIILAQISSASGVPLAAVLLLFLPDGPSTAFIHGLVLIIVGFFISWNAPATNNP 364
            FPNSGR+I AQ S   G   + VLL  +P   ++ +I  + L ++G  I+W  PA N+P
Sbjct: 312 VFPNSGRLICAQFSVFMGAMFSIVLLRMIPQSVNSFYIFLVTLFLMGLTITWCGPAINSP 371

Query: 365 IFAEIVPEKSRTSVYALDRSFESILSSFAPPVVGILAQHVYGYKPVQKGSSESEEIATDR 424
           I AEIVP K RT VYA DR+ E   SSF  P+VGI+++ ++G+      +   + +    
Sbjct: 372 ILAEIVPAKHRTMVYAFDRALEVTFSSFGAPLVGIMSEKLFGF-----DAKGIDHVNDSG 431

Query: 425 QNAASLARALYTAIGIPLALCCLIYSFLYCTYPRDRERARMEVLIESEM 459
           + A +L + +   + +P  LCCL Y+ L+  + +DR+  R     E EM
Sbjct: 432 REAEALGKGIMWMMALPFGLCCLCYTPLHFLFRKDRKIDRTTSSREVEM 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LE773.5e-27894.63MFS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G895860 PE=4 SV... [more]
A0A1S3CRQ73.5e-27894.43high affinity nitrate transporter 2.5 OS=Cucumis melo OX=3656 GN=LOC103503585 PE... [more]
A0A5D3E4Z81.3e-26994.26High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1FJL71.6e-26792.59uncharacterized protein LOC111445948 OS=Cucurbita moschata OX=3662 GN=LOC1114459... [more]
A0A6J1CP318.1e-26791.57uncharacterized protein LOC111012796 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_038898006.11.1e-27895.39Na(+), Li(+), K(+)/H(+) antiporter [Benincasa hispida][more]
XP_004136402.17.3e-27894.63uncharacterized protein LOC101220496 [Cucumis sativus][more]
XP_008466033.17.3e-27894.43PREDICTED: high affinity nitrate transporter 2.5 [Cucumis melo][more]
KAA0038604.12.8e-26994.26high affinity nitrate transporter 2.5 [Cucumis melo var. makuwa] >TYK31203.1 hig... [more]
KAG6608079.13.4e-26792.59Protein spinster, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G78130.13.0e-21378.69Major facilitator superfamily protein [more]
AT5G10190.12.8e-18770.69Major facilitator superfamily protein [more]
AT4G36790.16.7e-10444.32Major facilitator superfamily protein [more]
AT2G18590.12.8e-8639.23Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 14..344
e-value: 1.1E-34
score: 119.9
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 228..451
e-value: 1.1E-13
score: 53.0
coord: 4..191
e-value: 2.5E-29
score: 104.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 6..446
NoneNo IPR availablePANTHERPTHR23505SPINSTERcoord: 1..515
NoneNo IPR availablePANTHERPTHR23505:SF73SUBFAMILY NOT NAMEDcoord: 1..515
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 7..445
score: 20.544041
IPR044770Protein spinster-likeCDDcd17328MFS_spinster_likecoord: 8..434
e-value: 4.44405E-83
score: 261.787

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G023460.1Lsi04G023460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity