Lsi04G021390 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G021390 vs. ExPASy Swiss-Prot
Match: Q3V384 (AFG1-like ATPase OS=Mus musculus OX=10090 GN=Afg1l PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 6.6e-29
Identity = 136/578 (23.53%), Postives = 218/578 (37.72%), Query Frame = 0

Query: 258 PRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQ 317
           P+GLY+YG+VG+GKTM+MDMFY   E   K ++R HFH                      
Sbjct: 129 PKGLYVYGDVGTGKTMVMDMFYAYVE--TKRKKRVHFH---------------------G 188

Query: 318 AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLA-AEEKYKQEVQMKNIL 377
            ML ++  +H + ++  + K+             F  K  + +A   E+  QE       
Sbjct: 189 FMLDVHRRIHHLKQSLPKRKAG------------FMAKSYDPIAPIAEEISQE------- 248

Query: 378 PAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPK 437
                             S+LCFDE Q  D+   + L  +   L   G V+VATSNR P+
Sbjct: 249 -----------------TSLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPE 308

Query: 438 DLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIPLSSEKMFQNKLKEHVYQQST 497
           DL +                                                        
Sbjct: 309 DLYK-------------------------------------------------------- 368

Query: 498 HRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNASSASLASTVLKPQLLVVPIW 557
                                                                       
Sbjct: 369 ------------------------------------------------------------ 428

Query: 558 SRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDIFQKFVTKLEEHCEFVLIGSE 617
                                            +G+Q+  F  F+  L+E+C+ + + S 
Sbjct: 429 ---------------------------------NGLQRANFVPFIAVLKEYCDTLQLDSG 478

Query: 618 IDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQLGGQITSETIPVMFGRKLEVS 677
           +DYR+   + +     Y+   +       +K++ E+ +Q    +TS  I  + GR+L ++
Sbjct: 489 VDYRK--RELAPAGKLYYLTSEADVEAVVDKLFDEL-AQKQNDLTSPRILKVQGRELRLN 478

Query: 678 ESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIPMMSMRIRDKARRFITLIDEL 737
           ++C  VA   F+ LC +P+GA+DY+ L+KN+ TV I +IP  S+  R +ARRFITLID  
Sbjct: 549 KACGSVADCTFEELCERPLGASDYLELSKNFDTVIIRNIPQFSLAKRTQARRFITLIDNF 478

Query: 738 YNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAP 797
           Y+    + C A+  I  LF          L   Q ++E++  ++  + L       +   
Sbjct: 609 YDFKVRIICSASAPISSLF----------LHQHQ-DSESDQSRILMDDL------GLSQD 478

Query: 798 TAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY-LEGVRN 834
           +A +SM +G+EE+FAF+R +SRL EMQT  Y +EG R+
Sbjct: 669 SAGLSMFTGEEEIFAFQRTISRLTEMQTEQYWIEGDRS 478

BLAST of Lsi04G021390 vs. ExPASy Swiss-Prot
Match: Q32PX9 (AFG1-like ATPase OS=Rattus norvegicus OX=10116 GN=Afg1l PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 8.7e-29
Identity = 136/578 (23.53%), Postives = 219/578 (37.89%), Query Frame = 0

Query: 258 PRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQ 317
           P+GLY+YG+VG+GKTM+MDMFY   E  VK ++R HFH                      
Sbjct: 129 PKGLYVYGDVGTGKTMVMDMFYEYVE--VKSKRRVHFH---------------------G 188

Query: 318 AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLA-AEEKYKQEVQMKNIL 377
            ML +++ +H++ ++  + K            + F  K  + +A   E+  QE       
Sbjct: 189 FMLDVHKRIHRLKQSLPKRK------------VGFMAKSYDPIAPIAEEISQE------- 248

Query: 378 PAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPK 437
                            AS+LCFDE Q  D+   + L  +   L   G V+VATSNR P+
Sbjct: 249 -----------------ASLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPE 308

Query: 438 DLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIPLSSEKMFQNKLKEHVYQQST 497
           DL +                                                        
Sbjct: 309 DLYK-------------------------------------------------------- 368

Query: 498 HRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNASSASLASTVLKPQLLVVPIW 557
                                                                       
Sbjct: 369 ------------------------------------------------------------ 428

Query: 558 SRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDIFQKFVTKLEEHCEFVLIGSE 617
                                            +G+Q+  F  F+  L+E+C  V + S 
Sbjct: 429 ---------------------------------NGLQRANFVPFIAVLKEYCNTVQLDSG 478

Query: 618 IDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQLGGQITSETIPVMFGRKLEVS 677
           +DYR+     +    +     D  +V   +K++ E+ +Q    +TS  I  + GR+L ++
Sbjct: 489 VDYRKRELTPAGKLYYLTSEADVGTV--MDKLFDEL-AQKQNDLTSPRILKVQGRELRLN 478

Query: 678 ESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIPMMSMRIRDKARRFITLIDEL 737
           ++C  VA   F+ LC +P+GA+DY+ L+KN+ TV I +IP  S+  R + RRFITLID  
Sbjct: 549 KACGTVADCTFEELCERPLGASDYLELSKNFDTVIIRNIPQFSLAKRTQVRRFITLIDNF 478

Query: 738 YNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAP 797
           Y+    + C A+  I  LF    +           ++E++  ++  + L       +   
Sbjct: 609 YDFKVRIICSASVPISSLFVYQHQ-----------DSESDQSRVLMDDL------GLSQD 478

Query: 798 TAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY-LEGVRN 834
           +A +SM +G+EE+F+F+R +SRL EMQT  Y +EG R+
Sbjct: 669 SAGLSMFTGEEEIFSFQRTLSRLTEMQTEQYWIEGDRS 478

BLAST of Lsi04G021390 vs. ExPASy Swiss-Prot
Match: Q8WV93 (AFG1-like ATPase OS=Homo sapiens OX=9606 GN=AFG1L PE=1 SV=2)

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-28
Identity = 130/583 (22.30%), Postives = 211/583 (36.19%), Query Frame = 0

Query: 245 DSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFR 304
           + L  +  S    PRGLY+YG+VG+GKTM+MDMFY   E  +K ++R HFH         
Sbjct: 116 EGLFSKLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVE--MKRKKRVHFH--------- 175

Query: 305 IGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEE 364
                         ML +++ +H++ K  + ++       S+    P   ++ E      
Sbjct: 176 ------------GFMLDVHKRIHRL-KQSLPKRKPGFMAKSYDPIAPIAEEISE------ 235

Query: 365 KYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIG 424
                                        A +LCFDE Q  D+   + L  +   L   G
Sbjct: 236 ----------------------------EACLLCFDEFQVTDIADAMILKQLFENLFKNG 295

Query: 425 TVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIPLSSEKMFQ 484
            V+VATSNR P+DL +                                            
Sbjct: 296 VVVVATSNRPPEDLYK-------------------------------------------- 355

Query: 485 NKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNASSASLAST 544
                                                                       
Sbjct: 356 ------------------------------------------------------------ 415

Query: 545 VLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDIFQKFVTKL 604
                                                        +G+Q+  F  F+  L
Sbjct: 416 ---------------------------------------------NGLQRANFVPFIAVL 472

Query: 605 EEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQLGGQITSET 664
           +E+C  V + S IDYR+     +    +     D  +V   +K++ E+ +Q    +T   
Sbjct: 476 KEYCNTVQLDSGIDYRKRELPAAGKLYYLTSEADVEAV--MDKLFDEL-AQKQNDLTRPR 472

Query: 665 IPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIPMMSMRIRD 724
           I  + GR+L ++++C  VA   F+ LC +P+GA+DY+ L+KN+ T+F+ +IP  ++  R 
Sbjct: 536 ILKVQGRELRLNKACGTVADCTFEELCERPLGASDYLELSKNFDTIFLRNIPQFTLANRT 472

Query: 725 KARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGGKLRRNV 784
           + RRFITLID  Y+    + C A+T I  LF                  +    +L ++ 
Sbjct: 596 QGRRFITLIDNFYDLKVRIICSASTPISSLFL----------------HQHHDSELEQSR 472

Query: 785 LVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY 828
           ++  ++G        +SM +G+EE+FAF+R +SRL EMQT  Y
Sbjct: 656 ILMDDLGLSQDSAEGLSMFTGEEEIFAFQRTISRLTEMQTEQY 472

BLAST of Lsi04G021390 vs. ExPASy Swiss-Prot
Match: P46441 (Putative ATPase N2B OS=Haematobia irritans OX=7368 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.5e-25
Identity = 79/240 (32.92%), Postives = 121/240 (50.42%), Query Frame = 0

Query: 590 DGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMW 649
           +G+Q+  F  F+  L   C    + S +DYRR IAQ      +YF      +  + E+M+
Sbjct: 239 NGLQRSNFIPFIGVLLNRCNVAAMDSGVDYRR-IAQSG--DTNYFVTTQTDAKSQMERMF 298

Query: 650 VEVTSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHT 709
             + SQ    I   TI   FGR L    +C  V    F+ LC +P+G +DYI + + +HT
Sbjct: 299 KILCSQENDIIRPRTI-THFGRDLTFQRTCGQVLDSNFEELCNRPLGGSDYIQIGQFFHT 358

Query: 710 VFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESF 769
           V I D+P +++ ++ + RRFITLID LY++   +   A   ++ LF  T++    DL   
Sbjct: 359 VLIHDVPQLTLLLKSQMRRFITLIDTLYDNRVRVVISAEVPLDQLFSFTDKPK--DLADE 418

Query: 770 QFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLE 829
           Q           R ++ +  +G         S+ +G+EEMFAF R +SRL EMQ   Y E
Sbjct: 419 Q-----------RMLMDDLKLGDTDTS---ASVFTGEEEMFAFDRTISRLYEMQKKEYWE 458

HSP 2 Score: 52.8 bits (125), Expect = 2.3e-05
Identity = 23/39 (58.97%), Postives = 31/39 (79.49%), Query Frame = 0

Query: 257 APRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFH 296
           AP+G+YIYG+VG GKT LMDMFY+  + + K +QR HF+
Sbjct: 109 APKGMYIYGSVGGGKTTLMDMFYSCCDDIPK-KQRVHFN 146

BLAST of Lsi04G021390 vs. ExPASy Swiss-Prot
Match: Q5TYS0 (Lactation elevated protein 1 homolog B OS=Danio rerio OX=7955 GN=lace1b PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 9.3e-23
Identity = 118/575 (20.52%), Postives = 206/575 (35.83%), Query Frame = 0

Query: 253 SAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFY 312
           S PH P+G YIYGNVG+GKTMLMD+FY+  E   + ++R HF+                 
Sbjct: 147 SKPHPPQGYYIYGNVGTGKTMLMDLFYSFVEN--RRKKRVHFN----------------- 206

Query: 313 SIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQM 372
                 ML ++  +HK+ ++  + +  + ++   I  +  +        AEE        
Sbjct: 207 ----GFMLDVHRRIHKLKQSLPKRRIGKMTMYDPIFPVAME-------IAEE-------- 266

Query: 373 KNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSN 432
                                  ++CFDE Q VD+   + L  +   L   G V+VATSN
Sbjct: 267 ---------------------TCLICFDEFQVVDIADAMILKQLFEGLFKCGVVVVATSN 326

Query: 433 RAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIPLSSEKMFQNKLKEHVY 492
           R P++L +                                                    
Sbjct: 327 RPPEELYK---------------------------------------------------- 386

Query: 493 QQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNASSASLASTVLKPQLLV 552
                                                                       
Sbjct: 387 ------------------------------------------------------------ 446

Query: 553 VPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDIFQKFVTKLEEHCEFVL 612
                                                +G+Q+  F  F+  L+E+C  V 
Sbjct: 447 -------------------------------------NGLQRAAFVPFIGVLKEYCRIVS 494

Query: 613 IGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQLGGQITSETIPVMFGRK 672
           + + ID+R     +   +++Y    +  +      ++ E+  +    +T   +  + GR+
Sbjct: 507 LDTGIDFRT-REMKPAGRLYYI-SSEPDAENAVNALFEELAFR-QNDVTRPRVLNVQGRE 494

Query: 673 LEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIPMMSMRIRDKARRFITL 732
           + +S +C  +A  +F  LC QP+GA DY+ +A+ + TV I ++P + + ++D+ARRF TL
Sbjct: 567 VTLSRTCGTIADCSFQELCEQPLGAGDYLEIARCFDTVIIRNVPYLQLGMKDQARRFTTL 494

Query: 733 IDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGS 792
           ID  Y+    +  LA   ++ L    ++G +              G+  R+ L+   +G 
Sbjct: 627 IDNFYDQKVRVVMLADAPLDRLL---DQGQM-------------TGEEARDRLMLDELGL 494

Query: 793 VGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY 828
               +  +++ +  EE+FAF+R VSRL EMQT  Y
Sbjct: 687 TDEASKRITLFTADEEIFAFQRTVSRLAEMQTEQY 494

BLAST of Lsi04G021390 vs. ExPASy TrEMBL
Match: A0A6J1FDZ4 (AFG1-like ATPase OS=Cucurbita moschata OX=3662 GN=LOC111443118 PE=3 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 3.0e-306
Identity = 580/803 (72.23%), Postives = 588/803 (73.23%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSENRRFGFRNAVVLLVDL 117
           MKRGPSTSTI SLLSL+FTRQQSSK GVDP  VRFLSSS K                   
Sbjct: 1   MKRGPSTSTIYSLLSLKFTRQQSSKLGVDPLRVRFLSSSAKS------------------ 60

Query: 118 FRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKL 177
                      GLL+QYKHLVERGELQHDPFQERVAS+LESLLGRLEQYEKDMEEYHVKL
Sbjct: 61  ----------PGLLSQYKHLVERGELQHDPFQERVASELESLLGRLEQYEKDMEEYHVKL 120

Query: 178 ANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVS 237
           ANWKQ+RENERRRLLMEEAESKQQGDVWTSVNKRRSKL ETLMFRK+SENIEPGVGKWVS
Sbjct: 121 ANWKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGKWVS 180

Query: 238 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHE 297
           YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE
Sbjct: 181 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE 240

Query: 298 RCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 357
                                AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV
Sbjct: 241 ---------------------AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 300

Query: 358 KEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 417
           KEW+AAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI
Sbjct: 301 KEWVAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 360

Query: 418 VSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIP 477
           VSRLLS GTVLVATSNRAPKDLNQ                                    
Sbjct: 361 VSRLLSTGTVLVATSNRAPKDLNQ------------------------------------ 420

Query: 478 LSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNA 537
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 538 SSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDI 597
                                                                DGMQKDI
Sbjct: 481 -----------------------------------------------------DGMQKDI 540

Query: 598 FQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQL 657
           FQKFVTKLEEHC FV IGSEIDYRRFIAQRSFDQVHYFWP DCTSVGKFEKMWVE+TSQL
Sbjct: 541 FQKFVTKLEEHCAFVFIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEITSQL 600

Query: 658 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 717
           GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP
Sbjct: 601 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 605

Query: 718 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 777
           MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE
Sbjct: 661 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 605

Query: 778 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 837
           GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP
Sbjct: 721 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 605

Query: 838 YFQRREQESSVDPAPRFQFQPSS 860
           YFQRRE ES VD A RFQFQ SS
Sbjct: 781 YFQRREPESLVDSAARFQFQQSS 605

BLAST of Lsi04G021390 vs. ExPASy TrEMBL
Match: A0A6J1IG43 (AFG1-like ATPase OS=Cucurbita maxima OX=3661 GN=LOC111476619 PE=3 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 4.7e-304
Identity = 577/803 (71.86%), Postives = 585/803 (72.85%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKPSENRRFGFRNAVVLLVDL 117
           MKRG STSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K                   
Sbjct: 1   MKRGTSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKS------------------ 60

Query: 118 FRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKL 177
                      GLLNQYKHLVERGELQHDPFQERVAS+LESLLGRLEQYE+DMEEYHVKL
Sbjct: 61  ----------PGLLNQYKHLVERGELQHDPFQERVASELESLLGRLEQYERDMEEYHVKL 120

Query: 178 ANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVS 237
           ANWKQNRENERRRLLMEEAESKQ GDVWTSVNKRRSKL ETLMFRK+SENIEPGVGKWVS
Sbjct: 121 ANWKQNRENERRRLLMEEAESKQPGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGKWVS 180

Query: 238 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHE 297
           YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE
Sbjct: 181 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE 240

Query: 298 RCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 357
                                AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV
Sbjct: 241 ---------------------AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 300

Query: 358 KEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 417
           KEW+AAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI
Sbjct: 301 KEWVAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 360

Query: 418 VSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIP 477
           VSRLLS GTVLVATSNRAPKDLNQ                                    
Sbjct: 361 VSRLLSTGTVLVATSNRAPKDLNQ------------------------------------ 420

Query: 478 LSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNA 537
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 538 SSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDI 597
                                                                DGMQKDI
Sbjct: 481 -----------------------------------------------------DGMQKDI 540

Query: 598 FQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQL 657
           FQKFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQ+HYFWP DCTSVGKFEKMWVE+TSQL
Sbjct: 541 FQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQMHYFWPSDCTSVGKFEKMWVEITSQL 600

Query: 658 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 717
           GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP
Sbjct: 601 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 605

Query: 718 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 777
            MSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE
Sbjct: 661 TMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 605

Query: 778 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 837
           GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYL+GVRNLHP
Sbjct: 721 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLKGVRNLHP 605

Query: 838 YFQRREQESSVDPAPRFQFQPSS 860
           YFQRRE  S VD A RFQFQ SS
Sbjct: 781 YFQRREPASLVDSAARFQFQQSS 605

BLAST of Lsi04G021390 vs. ExPASy TrEMBL
Match: A0A0A0LHA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G878760 PE=3 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 8.9e-303
Identity = 589/859 (68.57%), Postives = 612/859 (71.25%), Query Frame = 0

Query: 1   MWLVSGKNTSSLSIFVAVFRYINDLELSIREIATFSSCRKTTRYTSNRRRRRNCDSKMKR 60
           M LVSGKNTSSLSIFV +F                    KT R+TS+RRRRRNCDS MKR
Sbjct: 1   MLLVSGKNTSSLSIFVLLF-------------------SKTIRHTSDRRRRRNCDSMMKR 60

Query: 61  GPSTSTISSLLSLRFTRQQ--SSKFGVDPC-VRFLSSSTKPSENRRFGFRNAVVLLVDLF 120
           G STS+ISSLLSLRFTRQQ  SSKFGV+P  +RFLSS TKP                   
Sbjct: 61  GLSTSSISSLLSLRFTRQQSSSSKFGVNPLPLRFLSSFTKP------------------- 120

Query: 121 RTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKLA 180
                     GLL++Y+ LVERGELQHDPFQERVAS+LESLLGRLEQYEKDMEEYHVKLA
Sbjct: 121 ---------PGLLDKYRVLVERGELQHDPFQERVASELESLLGRLEQYEKDMEEYHVKLA 180

Query: 181 NWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVSY 240
            WKQNRE ERRRLLMEEAESKQQGD   +VN+RRSKL+ETLMFRK SENIEPGVGKWVSY
Sbjct: 181 EWKQNREKERRRLLMEEAESKQQGD---AVNRRRSKLLETLMFRKKSENIEPGVGKWVSY 240

Query: 241 LNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHER 300
           LNRE+KLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMF+NATEG+VKHRQRYHFHE 
Sbjct: 241 LNREKKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFFNATEGIVKHRQRYHFHE- 300

Query: 301 CMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVK 360
                               AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVK
Sbjct: 301 --------------------AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVK 360

Query: 361 EWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIV 420
           EWLA EEKYKQEVQMKNILPAVADKFLVDQQAG VGASILCFDEIQTVDVFAIVALSGI+
Sbjct: 361 EWLAGEEKYKQEVQMKNILPAVADKFLVDQQAGHVGASILCFDEIQTVDVFAIVALSGII 420

Query: 421 SRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIPL 480
           SRLLS GTVLVATSNRAP DLNQ                                     
Sbjct: 421 SRLLSTGTVLVATSNRAPNDLNQ------------------------------------- 480

Query: 481 SSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNAS 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 540

Query: 541 SASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDIF 600
                                                               DGMQKDIF
Sbjct: 541 ----------------------------------------------------DGMQKDIF 600

Query: 601 QKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQLG 660
           QKFV KLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTS+ KFE MWVE+TSQL 
Sbjct: 601 QKFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSMRKFENMWVEITSQLE 639

Query: 661 GQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIPM 720
           GQITSETI VMFGRKLEV ESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFIS+IP+
Sbjct: 661 GQITSETIRVMFGRKLEVPESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISNIPI 639

Query: 721 MSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEG 780
           MSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEG
Sbjct: 721 MSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEG 639

Query: 781 GKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHPY 840
           GKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN+HPY
Sbjct: 781 GKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNVHPY 639

Query: 841 FQRREQESSVD-PAPRFQF 856
           FQRREQESSVD PAPRF+F
Sbjct: 841 FQRREQESSVDPPAPRFEF 639

BLAST of Lsi04G021390 vs. ExPASy TrEMBL
Match: A0A6J1C362 (AFG1-like ATPase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007517 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 6.2e-296
Identity = 567/813 (69.74%), Postives = 585/813 (71.96%), Query Frame = 0

Query: 48  RRRRRNCDSKMKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSENRRFGF 107
           RRRRRNC SKM+RGPSTSTISS LSLR  R QSS  G+D   VRFLSSS+KP        
Sbjct: 27  RRRRRNCVSKMRRGPSTSTISSFLSLRIAR-QSSNSGIDLIRVRFLSSSSKP-------- 86

Query: 108 RNAVVLLVDLFRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYE 167
                                GLL  Y+HLVERGELQHDPFQERVAS+LESLLGRLEQYE
Sbjct: 87  --------------------PGLLVHYRHLVERGELQHDPFQERVASELESLLGRLEQYE 146

Query: 168 KDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSEN 227
           KDME+YHVKLANWKQNRENERR+LLMEEAESKQQGDVWTSVNKR+SKLVETLMFR  SEN
Sbjct: 147 KDMEDYHVKLANWKQNRENERRKLLMEEAESKQQGDVWTSVNKRQSKLVETLMFRNKSEN 206

Query: 228 IEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVV 287
           IEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNAT+G+V
Sbjct: 207 IEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATKGIV 266

Query: 288 KHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSW 347
           KHR+RYHFHE                     AMLKINEDMH+IWKNQVREKSSQSSISSW
Sbjct: 267 KHRRRYHFHE---------------------AMLKINEDMHQIWKNQVREKSSQSSISSW 326

Query: 348 IMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVD 407
           IMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFLVDQQA QVGASILCFDEIQTVD
Sbjct: 327 IMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFLVDQQADQVGASILCFDEIQTVD 386

Query: 408 VFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMI 467
           VFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQ                          
Sbjct: 387 VFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQ-------------------------- 446

Query: 468 VSSVTIDFIPLSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSS 527
                                                                       
Sbjct: 447 ------------------------------------------------------------ 506

Query: 528 AFSFRNFRNASSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHI 587
                                                                       
Sbjct: 507 ------------------------------------------------------------ 566

Query: 588 ELGDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFE 647
              DGMQKDIFQKFV KLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPL+ TSVGKFE
Sbjct: 567 ---DGMQKDIFQKFVAKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLELTSVGKFE 626

Query: 648 KMWVEVTSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKN 707
           KMW E+T QLGGQITSETI VMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALA+N
Sbjct: 627 KMWAEMTRQLGGQITSETISVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAQN 640

Query: 708 YHTVFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDL 767
           YHTVFIS+IPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEG LFDL
Sbjct: 687 YHTVFISNIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGTLFDL 640

Query: 768 ESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPL 827
           ESFQFETEAEGGKLRRNVL EGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPL
Sbjct: 747 ESFQFETEAEGGKLRRNVLEEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPL 640

Query: 828 YLEGVRNLHPYFQRREQESSVDPAPRFQFQPSS 860
           YLEGVR+LHPYFQRREQES VD A +F  Q SS
Sbjct: 807 YLEGVRSLHPYFQRREQESLVDRATKFHSQLSS 640

BLAST of Lsi04G021390 vs. ExPASy TrEMBL
Match: A0A1S3CR63 (lactation elevated protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503816 PE=3 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 2.3e-290
Identity = 556/806 (68.98%), Postives = 580/806 (71.96%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQ--SSKFGVDPC-VRFLSSSTKPSENRRFGFRNAVVLLV 117
           MKRG STS+ISSLLSLRFTRQQ  SSKFGVDP  +RFLSS TKP                
Sbjct: 1   MKRGLSTSSISSLLSLRFTRQQFSSSKFGVDPLRLRFLSSFTKP---------------- 60

Query: 118 DLFRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHV 177
                        GLL++Y+ LVERGELQHDPFQERVAS+LESLLGRLEQYEKDME+YHV
Sbjct: 61  ------------PGLLDKYRVLVERGELQHDPFQERVASELESLLGRLEQYEKDMEDYHV 120

Query: 178 KLANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKW 237
           KLA W+QNRE ERRRLLMEEAESKQQGD   +VNKRRSKL ETLMFRK +ENIEPGVGKW
Sbjct: 121 KLAEWEQNREKERRRLLMEEAESKQQGD---AVNKRRSKLFETLMFRKKAENIEPGVGKW 180

Query: 238 VSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHF 297
           VSYLNRE+KLDSL+GRCP+APHAPRGLYIYGNVGSGKTMLMDMF+NAT+G+VKHRQRYHF
Sbjct: 181 VSYLNREKKLDSLIGRCPAAPHAPRGLYIYGNVGSGKTMLMDMFFNATQGIVKHRQRYHF 240

Query: 298 HERCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDT 357
           HE                     AMLKINEDMHKIWKNQVREKSSQSS SSWIMNLPFDT
Sbjct: 241 HE---------------------AMLKINEDMHKIWKNQVREKSSQSSFSSWIMNLPFDT 300

Query: 358 KVKEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALS 417
           KVKEWLA EEKYKQEVQMK+ILPAVADKFL+DQQAG VGASILCFDEIQTVDVFAIVALS
Sbjct: 301 KVKEWLAGEEKYKQEVQMKHILPAVADKFLIDQQAGHVGASILCFDEIQTVDVFAIVALS 360

Query: 418 GIVSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDF 477
           GI+SRLLS GTVLVATSNRAP DLNQ                                  
Sbjct: 361 GIISRLLSTGTVLVATSNRAPNDLNQ---------------------------------- 420

Query: 478 IPLSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFR 537
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 538 NASSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQK 597
                                                                  DGMQK
Sbjct: 481 -------------------------------------------------------DGMQK 540

Query: 598 DIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTS 657
           DIFQKFV KLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSV KFE MWVEVTS
Sbjct: 541 DIFQKFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVRKFENMWVEVTS 600

Query: 658 QLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISD 717
           QLGGQITSET+PVMFGRKLEV ESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFIS+
Sbjct: 601 QLGGQITSETVPVMFGRKLEVPESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISN 605

Query: 718 IPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETE 777
           IP+MSMRIRDKARRFITLIDELYNHHCCLFCLAATSIE+LFQGTEEGALFDLESFQFETE
Sbjct: 661 IPIMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEELFQGTEEGALFDLESFQFETE 605

Query: 778 AEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNL 837
           AEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN+
Sbjct: 721 AEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNI 605

Query: 838 HPYFQRREQESSVD-PAPRFQFQPSS 860
           HPYFQRREQESSVD PAPRFQF   S
Sbjct: 781 HPYFQRREQESSVDPPAPRFQFHQLS 605

BLAST of Lsi04G021390 vs. NCBI nr
Match: KAG7024466.1 (AFG1-like ATPase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 622/861 (72.24%), Postives = 637/861 (73.98%), Query Frame = 0

Query: 2   WLVSGKNTSSLSIFVAVFRYINDLELSIR--EIATFSSCRKTTRYTSNRRRRRNCDSKMK 61
           WLVSGKN S  SIFVAV  +        R  ++  F    +TTRYTS RRRRRNCDS+MK
Sbjct: 18  WLVSGKNKSLFSIFVAVCSFGASTIWRSRFWKLQRFLLV-ETTRYTSRRRRRRNCDSEMK 77

Query: 62  RGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKPSENRRFGFRNAVVLLVDLFR 121
           RGPSTSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K                     
Sbjct: 78  RGPSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKS-------------------- 137

Query: 122 TCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKLAN 181
                    GLL+QYKHLVERGELQHDPFQERVAS+LESLLGRLEQYEKDMEEYHVKLAN
Sbjct: 138 --------PGLLSQYKHLVERGELQHDPFQERVASELESLLGRLEQYEKDMEEYHVKLAN 197

Query: 182 WKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYL 241
           WKQ+RENERRRLLMEEAESKQQGDVWTSVNKRRSKL ETLMFRK+SENIEPGVGKWVSYL
Sbjct: 198 WKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGKWVSYL 257

Query: 242 NRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERC 301
           NRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE  
Sbjct: 258 NRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE-- 317

Query: 302 MPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKE 361
                 IGIG+Q YSI+WQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKE
Sbjct: 318 ------IGIGIQVYSIIWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKE 377

Query: 362 WLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVS 421
           W+AAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVS
Sbjct: 378 WVAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVS 437

Query: 422 RLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIPLS 481
           RLLS GTVLVATSNRAPKDLNQ                                      
Sbjct: 438 RLLSTGTVLVATSNRAPKDLNQ-------------------------------------- 497

Query: 482 SEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNASS 541
                                                                       
Sbjct: 498 ------------------------------------------------------------ 557

Query: 542 ASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDIFQ 601
                                                              DGMQKDIFQ
Sbjct: 558 ---------------------------------------------------DGMQKDIFQ 617

Query: 602 KFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQLGG 661
           KFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQVHYFWP DCTSVGKFEKMWVE+TSQLGG
Sbjct: 618 KFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEITSQLGG 677

Query: 662 QITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIPMM 721
           QITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP+M
Sbjct: 678 QITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIPVM 692

Query: 722 SMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGG 781
           SMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGG
Sbjct: 738 SMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGG 692

Query: 782 KLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHPYF 841
           KLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHPYF
Sbjct: 798 KLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHPYF 692

Query: 842 QRREQESSVDPAPRFQFQPSS 860
           QRRE ES VD A RFQFQ SS
Sbjct: 858 QRREPESLVDSAARFQFQQSS 692

BLAST of Lsi04G021390 vs. NCBI nr
Match: KAG6591578.1 (AFG1-like ATPase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 594/821 (72.35%), Postives = 606/821 (73.81%), Query Frame = 0

Query: 40  KTTRYTSNRRRRRNCDSKMKRGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKP 99
           +TTRYTS RRRRRNCDS+MKRGPSTSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K 
Sbjct: 42  ETTRYTSRRRRRRNCDSEMKRGPSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKS 101

Query: 100 SENRRFGFRNAVVLLVDLFRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESL 159
                                        GLL+QYKHLVERGELQHDPFQERVAS+LESL
Sbjct: 102 ----------------------------PGLLSQYKHLVERGELQHDPFQERVASELESL 161

Query: 160 LGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETL 219
           LGRLEQYEKD+EEYHVKLANWKQ+RENERRRLLMEEAESKQQGDVWTSVNKRRSKL ETL
Sbjct: 162 LGRLEQYEKDLEEYHVKLANWKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETL 221

Query: 220 MFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMF 279
           MFRK+SENIEPGVGKWVSYLNRERKLDSLVGRCPSAP+APRGLYIYGNVGSGKTMLMDMF
Sbjct: 222 MFRKSSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPNAPRGLYIYGNVGSGKTMLMDMF 281

Query: 280 YNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKS 339
           YNATEG+VKHRQRYHFHE                     AMLKINEDMHKIWKNQVREKS
Sbjct: 282 YNATEGIVKHRQRYHFHE---------------------AMLKINEDMHKIWKNQVREKS 341

Query: 340 SQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILC 399
           SQSSISSWIMNLPFDTKVKEW+AAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILC
Sbjct: 342 SQSSISSWIMNLPFDTKVKEWVAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILC 401

Query: 400 FDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDP 459
           FDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQ                  
Sbjct: 402 FDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQ------------------ 461

Query: 460 DVTNPKMIVSSVTIDFIPLSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRE 519
                                                                       
Sbjct: 462 ------------------------------------------------------------ 521

Query: 520 SWNSLPSSAFSFRNFRNASSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLS 579
                                                                       
Sbjct: 522 ------------------------------------------------------------ 581

Query: 580 YPSSDRHIELGDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLD 639
                      DGMQKDIFQKFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQVHYFWP D
Sbjct: 582 -----------DGMQKDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFWPSD 641

Query: 640 CTSVGKFEKMWVEVTSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAA 699
           CTSVGKFEKMWVE+TSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAA
Sbjct: 642 CTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAA 664

Query: 700 DYIALAKNYHTVFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGT 759
           DYIALAKNYHTVFISDIP+MSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGT
Sbjct: 702 DYIALAKNYHTVFISDIPVMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGT 664

Query: 760 EEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSR 819
           EEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSR
Sbjct: 762 EEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSR 664

Query: 820 LIEMQTPLYLEGVRNLHPYFQRREQESSVDPAPRFQFQPSS 860
           LIEMQTPLYLEGVRNLHPYFQRRE ES VD A RFQFQ SS
Sbjct: 822 LIEMQTPLYLEGVRNLHPYFQRREPESLVDSAARFQFQQSS 664

BLAST of Lsi04G021390 vs. NCBI nr
Match: XP_022936535.1 (AFG1-like ATPase [Cucurbita moschata] >XP_022936536.1 AFG1-like ATPase [Cucurbita moschata])

HSP 1 Score: 1060.8 bits (2742), Expect = 6.1e-306
Identity = 580/803 (72.23%), Postives = 588/803 (73.23%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSENRRFGFRNAVVLLVDL 117
           MKRGPSTSTI SLLSL+FTRQQSSK GVDP  VRFLSSS K                   
Sbjct: 1   MKRGPSTSTIYSLLSLKFTRQQSSKLGVDPLRVRFLSSSAKS------------------ 60

Query: 118 FRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKL 177
                      GLL+QYKHLVERGELQHDPFQERVAS+LESLLGRLEQYEKDMEEYHVKL
Sbjct: 61  ----------PGLLSQYKHLVERGELQHDPFQERVASELESLLGRLEQYEKDMEEYHVKL 120

Query: 178 ANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVS 237
           ANWKQ+RENERRRLLMEEAESKQQGDVWTSVNKRRSKL ETLMFRK+SENIEPGVGKWVS
Sbjct: 121 ANWKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGKWVS 180

Query: 238 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHE 297
           YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE
Sbjct: 181 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE 240

Query: 298 RCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 357
                                AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV
Sbjct: 241 ---------------------AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 300

Query: 358 KEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 417
           KEW+AAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI
Sbjct: 301 KEWVAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 360

Query: 418 VSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIP 477
           VSRLLS GTVLVATSNRAPKDLNQ                                    
Sbjct: 361 VSRLLSTGTVLVATSNRAPKDLNQ------------------------------------ 420

Query: 478 LSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNA 537
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 538 SSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDI 597
                                                                DGMQKDI
Sbjct: 481 -----------------------------------------------------DGMQKDI 540

Query: 598 FQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQL 657
           FQKFVTKLEEHC FV IGSEIDYRRFIAQRSFDQVHYFWP DCTSVGKFEKMWVE+TSQL
Sbjct: 541 FQKFVTKLEEHCAFVFIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEITSQL 600

Query: 658 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 717
           GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP
Sbjct: 601 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 605

Query: 718 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 777
           MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE
Sbjct: 661 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 605

Query: 778 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 837
           GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP
Sbjct: 721 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 605

Query: 838 YFQRREQESSVDPAPRFQFQPSS 860
           YFQRRE ES VD A RFQFQ SS
Sbjct: 781 YFQRREPESLVDSAARFQFQQSS 605

BLAST of Lsi04G021390 vs. NCBI nr
Match: XP_038900047.1 (AFG1-like ATPase isoform X1 [Benincasa hispida] >XP_038900048.1 AFG1-like ATPase isoform X1 [Benincasa hispida])

HSP 1 Score: 1057.4 bits (2733), Expect = 6.7e-305
Identity = 578/803 (71.98%), Postives = 588/803 (73.23%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSENRRFGFRNAVVLLVDL 117
           MKRGPSTSTISSLLSLRFTRQQSSKFG DP  VRFLSSSTKP                  
Sbjct: 1   MKRGPSTSTISSLLSLRFTRQQSSKFGADPLRVRFLSSSTKP------------------ 60

Query: 118 FRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKL 177
                      GLLN+Y+ LVERGELQHDPFQERVAS+LESLLGRLEQYEKDMEEYHVKL
Sbjct: 61  ----------PGLLNKYRLLVERGELQHDPFQERVASELESLLGRLEQYEKDMEEYHVKL 120

Query: 178 ANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVS 237
           ANWKQNRE ERRRLLMEEAESKQQG+VWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVS
Sbjct: 121 ANWKQNREKERRRLLMEEAESKQQGNVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVS 180

Query: 238 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHE 297
           YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE
Sbjct: 181 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE 240

Query: 298 RCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 357
                                AMLKINEDMH IWKNQ+REKSSQSSISSWIMNLPFDTKV
Sbjct: 241 ---------------------AMLKINEDMHNIWKNQMREKSSQSSISSWIMNLPFDTKV 300

Query: 358 KEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 417
           KEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI
Sbjct: 301 KEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 360

Query: 418 VSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIP 477
           VSRLLS GTVLVATSNRAPKDLNQ                                    
Sbjct: 361 VSRLLSTGTVLVATSNRAPKDLNQ------------------------------------ 420

Query: 478 LSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNA 537
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 538 SSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDI 597
                                                                DGMQK+I
Sbjct: 481 -----------------------------------------------------DGMQKEI 540

Query: 598 FQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQL 657
           FQ FV KLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLD TSVGKFE MWVE+TSQL
Sbjct: 541 FQNFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDSTSVGKFENMWVEITSQL 600

Query: 658 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 717
           GGQITS+TIPVMFGRKLEVSE+CNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP
Sbjct: 601 GGQITSKTIPVMFGRKLEVSENCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 605

Query: 718 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 777
           MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE
Sbjct: 661 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 605

Query: 778 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 837
           GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP
Sbjct: 721 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 605

Query: 838 YFQRREQESSVDPAPRFQFQPSS 860
           YFQRREQESSVDP    QFQ SS
Sbjct: 781 YFQRREQESSVDPTAGLQFQESS 605

BLAST of Lsi04G021390 vs. NCBI nr
Match: XP_023536384.1 (AFG1-like ATPase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1056.2 bits (2730), Expect = 1.5e-304
Identity = 578/803 (71.98%), Postives = 586/803 (72.98%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKPSENRRFGFRNAVVLLVDL 117
           MKRGPSTSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K                   
Sbjct: 1   MKRGPSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKS------------------ 60

Query: 118 FRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKL 177
                      GLL+QYKHLVERGELQHDPFQERVAS+LESLLGRLEQYEKDMEEYHVKL
Sbjct: 61  ----------PGLLSQYKHLVERGELQHDPFQERVASELESLLGRLEQYEKDMEEYHVKL 120

Query: 178 ANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVS 237
           ANWKQ+RENERRRLLMEEAESKQQGDVWTSVN+RRSKL ETLMFRK+SENIEPGVGKWVS
Sbjct: 121 ANWKQSRENERRRLLMEEAESKQQGDVWTSVNRRRSKLAETLMFRKSSENIEPGVGKWVS 180

Query: 238 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHE 297
           YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE
Sbjct: 181 YLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE 240

Query: 298 RCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKV 357
                                AMLKINEDMH IWKNQVREKSSQSSISSWIMNLPFDTKV
Sbjct: 241 ---------------------AMLKINEDMHNIWKNQVREKSSQSSISSWIMNLPFDTKV 300

Query: 358 KEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 417
           KEW+AAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI
Sbjct: 301 KEWVAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGI 360

Query: 418 VSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTNPKMIVSSVTIDFIP 477
           VSRLLS GTVLVATSNRAPKDLNQ                                    
Sbjct: 361 VSRLLSTGTVLVATSNRAPKDLNQ------------------------------------ 420

Query: 478 LSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNSLPSSAFSFRNFRNA 537
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 538 SSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSSDRHIELGDGMQKDI 597
                                                                DGMQKDI
Sbjct: 481 -----------------------------------------------------DGMQKDI 540

Query: 598 FQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWVEVTSQL 657
           FQKFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQVHYFWP DCTSVGKFEKMWVE+TSQL
Sbjct: 541 FQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEITSQL 600

Query: 658 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 717
           GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP
Sbjct: 601 GGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDIP 605

Query: 718 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 777
           MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE
Sbjct: 661 MMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAE 605

Query: 778 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 837
           GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP
Sbjct: 721 GGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLHP 605

Query: 838 YFQRREQESSVDPAPRFQFQPSS 860
           YFQRRE ES VD A R  FQ SS
Sbjct: 781 YFQRREPESLVDSAARLHFQQSS 605

BLAST of Lsi04G021390 vs. TAIR 10
Match: AT2G25530.1 (AFG1-like ATPase family protein )

HSP 1 Score: 730.3 bits (1884), Expect = 1.8e-210
Identity = 401/800 (50.12%), Postives = 485/800 (60.62%), Query Frame = 0

Query: 43  RYTSNRRRRRNCDSKMKRGPSTSTISSLLSLRFTRQQSSKFGVDPCVRFLSSSTKPSENR 102
           R  S +RR  N   K +  P+ S IS++L L F+ ++    G+    R  S+S  P +  
Sbjct: 51  RKASLQRRNHNRSLKQQIMPNVSQISTVLRLAFSCKRRLSNGLHFVARSYSTSDPPKQP- 110

Query: 103 RFGFRNAVVLLVDLFRTCNMRSILSGLLNQYKHLVERGELQHDPFQERVASKLESLLGRL 162
                                   SG L  Y  LVE+G LQHDP+QE+V S  E+L GRL
Sbjct: 111 ------------------------SGPLTNYSKLVEQGRLQHDPYQEKVVSAFENLFGRL 170

Query: 163 EQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRK 222
           E +EK ME+YHV+LA W++ RE ERR+L++EEAE K+   +W SVNK+  KL+   +  +
Sbjct: 171 EHFEKQMEDYHVRLAEWEKKREEERRKLMVEEAEKKEDDGMWASVNKQGQKLLGRWVLGR 230

Query: 223 NSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNAT 282
              N+EPGVGKWVSYLNRERKLDS+VG  P+ P AP+GLYIYGNVG GKTMLMDMFY AT
Sbjct: 231 RQMNVEPGVGKWVSYLNRERKLDSIVGSRPAVPPAPKGLYIYGNVGCGKTMLMDMFYGAT 290

Query: 283 EGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSS 342
           +G+++HRQR+HFHE                     AMLKINE MHK WK    EK  Q S
Sbjct: 291 DGIIRHRQRFHFHE---------------------AMLKINEQMHKYWKENGAEKPMQYS 350

Query: 343 ISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEI 402
           ISSWIMNLP D KVKEWLA EE YKQ++QMK+ILPAVADKFLVDQQ+ + GASILCFDEI
Sbjct: 351 ISSWIMNLPVDEKVKEWLAGEEFYKQQLQMKHILPAVADKFLVDQQSSKKGASILCFDEI 410

Query: 403 QTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQMFGIGVHLTCFFGMRRDPDVTN 462
           QTVDVFAIVALSGI+SRLL+ GTVLVATSNRAP++LNQ                      
Sbjct: 411 QTVDVFAIVALSGIMSRLLTTGTVLVATSNRAPRELNQ---------------------- 470

Query: 463 PKMIVSSVTIDFIPLSSEKMFQNKLKEHVYQQSTHRTRNKKKSNHREPKSTQHLRESWNS 522
                                                                       
Sbjct: 471 ------------------------------------------------------------ 530

Query: 523 LPSSAFSFRNFRNASSASLASTVLKPQLLVVPIWSRLANHFCLPFHCPLRSPNYLSYPSS 582
                                                                       
Sbjct: 531 ------------------------------------------------------------ 590

Query: 583 DRHIELGDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSV 642
                  DGMQK+IF KF++KLE+HCE + IGSE+DYRR  A+ S + VHY WPL+   +
Sbjct: 591 -------DGMQKEIFDKFISKLEKHCEIISIGSEVDYRRVAAKNSAENVHYLWPLNDAVL 650

Query: 643 GKFEKMWVEVTSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIA 702
            +FEKMW +VT Q GG+ITS T+PVMFGR +EV ESC+GVARF F++LCG+PVGAADYIA
Sbjct: 651 EEFEKMWRQVTDQYGGEITSATLPVMFGRTVEVPESCSGVARFTFEYLCGRPVGAADYIA 655

Query: 703 LAKNYHTVFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGA 762
           +AKNYHT+FISDIP MSM IRDKARRFITL+DELYNHHCCL   A T+I++LFQGT EG 
Sbjct: 711 VAKNYHTIFISDIPAMSMEIRDKARRFITLVDELYNHHCCLVSSAETAIDELFQGTAEGT 655

Query: 763 LFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEM 822
           LFDLESFQFETE E  +LRR+VL EG++ + G+P++IVSMLSG+EEMFAF RA SRLIEM
Sbjct: 771 LFDLESFQFETETEDSRLRRDVLAEGSISAAGSPSSIVSMLSGEEEMFAFARAASRLIEM 655

Query: 823 QTPLYLEGVRNLHPYFQRRE 843
           QTPLYLEGVR LHPYF +++
Sbjct: 831 QTPLYLEGVRFLHPYFHQQK 655

BLAST of Lsi04G021390 vs. TAIR 10
Match: AT4G30490.1 (AFG1-like ATPase family protein )

HSP 1 Score: 112.1 bits (279), Expect = 2.3e-24
Identity = 72/243 (29.63%), Postives = 120/243 (49.38%), Query Frame = 0

Query: 591 GMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSV--GKFEKM 650
           G+Q+D+F  F++ L+E      IGS +DYR+     S +Q  YF   D +++   KF ++
Sbjct: 276 GLQRDLFLPFISSLKERSVVHEIGSAVDYRKL---TSAEQGFYFIGKDLSTLLKQKFRQL 335

Query: 651 WVEVTSQLGGQITS--ETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKN 710
                  +G  + +  + + V+ GRKL++    NG A F F+ LC +P+GAADY  L K 
Sbjct: 336 -------IGDNVVARPQVVEVVMGRKLQIPLGANGCAYFPFEELCDRPLGAADYFGLFKK 395

Query: 711 YHTVFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDL 770
           +HT+ + +IP+  +  R  A RF+TL+D +Y +   L C A  + ++L +      +  +
Sbjct: 396 FHTLALDEIPVFGLHNRTAAYRFVTLVDVMYENRARLLCTAEANPQELLE-----KIITI 455

Query: 771 ESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPL 830
              +        + R+N + E              +    E  FA  R +SRL EM +  
Sbjct: 456 SEAKSMGPRTSSRSRKNDVTE--------------LCVDNELGFAKDRTISRLTEMNSKE 489

HSP 2 Score: 50.1 bits (118), Expect = 1.1e-05
Identity = 23/46 (50.00%), Postives = 33/46 (71.74%), Query Frame = 0

Query: 252 PSAPHAP-RGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHE 297
           P   ++P +GLY+YG VG+GKTMLMD+F++      K +QR HFH+
Sbjct: 154 PQTSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPCTWK-KQRIHFHD 198

HSP 3 Score: 37.0 bits (84), Expect = 9.3e-02
Identity = 20/45 (44.44%), Postives = 25/45 (55.56%), Query Frame = 0

Query: 394 ASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDL 439
           A +LC DE    DV   + L+ +   L S G +LVATSNR P  L
Sbjct: 228 AILLCLDEFMVTDVADALILNRLFGHLFSNGVILVATSNRNPDKL 272

BLAST of Lsi04G021390 vs. TAIR 10
Match: AT4G28070.2 (AFG1-like ATPase family protein )

HSP 1 Score: 111.7 bits (278), Expect = 3.0e-24
Identity = 75/240 (31.25%), Postives = 119/240 (49.58%), Query Frame = 0

Query: 591 GMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWV 650
           G+Q+D+F  F++ L+E C    IGS +DYR+     S ++  YF   D + + K +K  +
Sbjct: 248 GLQRDLFLPFISTLKERCVVREIGSSVDYRKL---TSAEEGFYFIGKDISGLLK-QKFQL 307

Query: 651 EVTSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTV 710
            V  Q  G    + + V+ GRKL+V  + +G A F F+ LC +P+GAADY+ L K +HT+
Sbjct: 308 LVGDQPAG---PQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLGLFKKFHTL 367

Query: 711 FISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQ 770
            +  +P   +  R  A RF+TL+D +Y +   L C A  S  +L +      + D +   
Sbjct: 368 ALEGVPFFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIV--TISDAQQIA 427

Query: 771 FETEAEGGKL-RRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLE 830
             T +   K    ++ V+  +G                  FA  R +SRL EM +  YLE
Sbjct: 428 PRTSSRSRKSDDPDLCVDNELG------------------FAKDRTISRLTEMNSKEYLE 460

HSP 2 Score: 45.1 bits (105), Expect = 3.4e-04
Identity = 20/37 (54.05%), Postives = 27/37 (72.97%), Query Frame = 0

Query: 259 RGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFH 296
           +GLY+YG VG+GKTMLMD+F++      +  QR HFH
Sbjct: 134 KGLYLYGGVGTGKTMLMDLFFHQLPASWR-TQRIHFH 169

HSP 3 Score: 37.0 bits (84), Expect = 9.3e-02
Identity = 20/45 (44.44%), Postives = 27/45 (60.00%), Query Frame = 0

Query: 394 ASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDL 439
           A +LC DE    DV   + L+ +   L + G +LVATSNRAP +L
Sbjct: 200 AILLCLDEFMVNDVADALILNRLFRHLFNNGIILVATSNRAPDNL 244

BLAST of Lsi04G021390 vs. TAIR 10
Match: AT4G28070.1 (AFG1-like ATPase family protein )

HSP 1 Score: 95.1 bits (235), Expect = 2.9e-19
Identity = 72/240 (30.00%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 591 GMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVGKFEKMWV 650
           G+Q+D+F  F++ L+E C    IGS +DYR+     S ++  YF   D + + K +K  +
Sbjct: 248 GLQRDLFLPFISTLKERCVVREIGSSVDYRKL---TSAEEGFYFIGKDISGLLK-QKFQL 307

Query: 651 EVTSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTV 710
            V  Q  G    + + V+ GRKL+V  + +G A F F+ LC +P+GAADY+ L       
Sbjct: 308 LVGDQPAG---PQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLGL------- 367

Query: 711 FISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQ 770
               +P   +  R  A RF+TL+D +Y +   L C A  S  +L +      + D +   
Sbjct: 368 --FSVPFFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIV--TISDAQQIA 427

Query: 771 FETEAEGGKL-RRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLE 830
             T +   K    ++ V+  +G                  FA  R +SRL EM +  YLE
Sbjct: 428 PRTSSRSRKSDDPDLCVDNELG------------------FAKDRTISRLTEMNSKEYLE 451

HSP 2 Score: 45.1 bits (105), Expect = 3.4e-04
Identity = 20/37 (54.05%), Postives = 27/37 (72.97%), Query Frame = 0

Query: 259 RGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFH 296
           +GLY+YG VG+GKTMLMD+F++      +  QR HFH
Sbjct: 134 KGLYLYGGVGTGKTMLMDLFFHQLPASWR-TQRIHFH 169

HSP 3 Score: 37.0 bits (84), Expect = 9.3e-02
Identity = 20/45 (44.44%), Postives = 27/45 (60.00%), Query Frame = 0

Query: 394 ASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDL 439
           A +LC DE    DV   + L+ +   L + G +LVATSNRAP +L
Sbjct: 200 AILLCLDEFMVNDVADALILNRLFRHLFNNGIILVATSNRAPDNL 244