Lsi04G021360 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G021360
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	chaperone protein DnaJ-like
Location	chr04: 28488121 .. 28489022 (-)
RNA-Seq Expression	Lsi04G021360
Synteny	Lsi04G021360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTATCATCCATATATAATCGCCTTTACCAAATCAACAATTTGTTTTCTTTCCTTAAAATATAAAAATAAAATAAAATAAAATCTATCCGCGCCAACTTGAGCCTAAATCAACCAATTAGAGGATATATGCATTTTGTCTTTAAAATTTTAAAATATCCAAAAATGAAAAACATTAATATATATATATATATTAACAGATATTTTTCGTAGTATAATCCATTAACAGCCAACCGTTCGGAATTTCAATGGCGTCTTCGTCTTTCTCGGCGTCGGCAAATTGCTTCAGTTCCAGAACTACAGTATCAGGTAACTCAAAATCATTTTCATTCTCGTAAAGATTTGCAGAAACATTTTTAGTGAACTACAAAAAACACTTGCAATTTATCAATCTTCTCACGAATTTCGCAGCAATTAGAGGTTGTAGTAATCAAAAGCTCAACCTAATTAACAATGGCTTCCATGATTATTCTTCCCCTGCTCGATTCCCTCATCTAAATGTCAAGGTTCTGCTTCTTCTCGATCGCCTTCTCCTGTTTGATTTTTGTTTGAAATGAAGCGATAATGTGTATATCATCTAAATTAGAGTCTGGAGAATGATTTGAAATGTTAAAATTAATGGAAATTGAAAATGTTTACTGTGTTTTCTGATCAATTAGGCTGCGAAGAATGATCGGAACACAAAACCTAATAGCGTGATTTGTGGCGATTGCGATGGAAATGGTGAGTTGATTTATGAGATTGGTATAGACGATTTAGGTTTTCCGAATGGCTGATGGTTTTGTAAAACTGAATGAAATAGGTGCTGTTCTTTGCTCACAATGCAAAGGAAATGGAGTTAATGCTGTTGATTTCTTCAATGGACAGTTCAAAGCCGGCGCATCCTGTTGGCTGTGCGGGTAA

mRNA sequence

TATTATCATCCATATATAATCGCCTTTACCAAATCAACAATTTGTTTTCTTTCCTTAAAATATAAAAATAAAATAAAATAAAATCTATCCGCGCCAACTTGAGCCTAAATCAACCAATTAGAGGATATATGCATTTTGTCTTTAAAATTTTAAAATATCCAAAAATGAAAAACATTAATATATATATATATATTAACAGATATTTTTCGTAGTATAATCCATTAACAGCCAACCGTTCGGAATTTCAATGGCGTCTTCGTCTTTCTCGGCGTCGGCAAATTGCTTCAGTTCCAGAACTACAGTATCAGCAATTAGAGGTTGTAGTAATCAAAAGCTCAACCTAATTAACAATGGCTTCCATGATTATTCTTCCCCTGCTCGATTCCCTCATCTAAATGTCAAGGCTGCGAAGAATGATCGGAACACAAAACCTAATAGCGTGATTTGTGGCGATTGCGATGGAAATGGTGCTGTTCTTTGCTCACAATGCAAAGGAAATGGAGTTAATGCTGTTGATTTCTTCAATGGACAGTTCAAAGCCGGCGCATCCTGTTGGCTGTGCGGGTAA

Coding sequence (CDS)

ATGGCGTCTTCGTCTTTCTCGGCGTCGGCAAATTGCTTCAGTTCCAGAACTACAGTATCAGCAATTAGAGGTTGTAGTAATCAAAAGCTCAACCTAATTAACAATGGCTTCCATGATTATTCTTCCCCTGCTCGATTCCCTCATCTAAATGTCAAGGCTGCGAAGAATGATCGGAACACAAAACCTAATAGCGTGATTTGTGGCGATTGCGATGGAAATGGTGCTGTTCTTTGCTCACAATGCAAAGGAAATGGAGTTAATGCTGTTGATTTCTTCAATGGACAGTTCAAAGCCGGCGCATCCTGTTGGCTGTGCGGGTAA

Protein sequence

MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNTKPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG

Homology

[Close]

BLAST of Lsi04G021360 vs. ExPASy Swiss-Prot
Match: Q9SN73 (Protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BSD2 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.6e-17
Identity = 39/63 (61.90%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 44  ARFPHLNVKAAKND-RNTKPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASC 103
           +R     VKAA N+ + TKPNS++C +C+G G V CSQCKG GVN +D FNGQFKAGA C
Sbjct: 48  SRSQSFEVKAANNNPQGTKPNSLVCANCEGEGCVACSQCKGGGVNLIDHFNGQFKAGALC 107

Query: 104 WLC 106
           WLC
Sbjct: 108 WLC 110

[Close]

BLAST of Lsi04G021360 vs. ExPASy Swiss-Prot
Match: Q9XF14 (Protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic OS=Zea mays OX=4577 GN=BSD2 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 2.6e-15
Identity = 32/61 (52.46%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 49  LNVKAAKNDRNTKPN----SVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWL 106
           +  KA +ND++ K +    S++C DC+GNGA++C++C+GNGVN+VD+F G+FKAG+ CWL
Sbjct: 40  IKTKATENDQSAKKHQKVKSILCQDCEGNGAIVCTKCEGNGVNSVDYFEGRFKAGSLCWL 99

[Close]

BLAST of Lsi04G021360 vs. ExPASy TrEMBL
Match: A0A5D3E725 (Chaperone protein DnaJ-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007410 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 2.8e-49
Identity = 97/106 (91.51%), Postives = 101/106 (95.28%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNT 60
           MASSSFS SANCFSS TT+SA+R CSNQKLNLI+NGFHDYSSPARFPHL +KAAKNDRNT
Sbjct: 1   MASSSFSLSANCFSSTTTLSALRDCSNQKLNLIHNGFHDYSSPARFPHLILKAAKNDRNT 60

Query: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGKSCWLCG 106

[Close]

BLAST of Lsi04G021360 vs. ExPASy TrEMBL
Match: A0A1S3CSH4 (chaperone protein DnaJ-like OS=Cucumis melo OX=3656 GN=LOC103503827 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 2.8e-49
Identity = 97/106 (91.51%), Postives = 101/106 (95.28%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNT 60
           MASSSFS SANCFSS TT+SA+R CSNQKLNLI+NGFHDYSSPARFPHL +KAAKNDRNT
Sbjct: 1   MASSSFSLSANCFSSTTTLSALRDCSNQKLNLIHNGFHDYSSPARFPHLILKAAKNDRNT 60

Query: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGKSCWLCG 106

[Close]

BLAST of Lsi04G021360 vs. ExPASy TrEMBL
Match: A0A5A7TB44 (Chaperone protein DnaJ-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G003640 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 8.2e-49
Identity = 97/106 (91.51%), Postives = 100/106 (94.34%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNT 60
           MASSSFS SANCFSS TT+SAIR CSNQKLNLI+N FHDYSSPARFPHL +KAAKNDRNT
Sbjct: 1   MASSSFSLSANCFSSTTTLSAIRDCSNQKLNLIHNAFHDYSSPARFPHLILKAAKNDRNT 60

Query: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGKSCWLCG 106

[Close]

BLAST of Lsi04G021360 vs. ExPASy TrEMBL
Match: A0A0A0LDP8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G878740 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.5e-47
Identity = 96/107 (89.72%), Postives = 100/107 (93.46%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSA-IRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRN 60
           MASSSFS SANCFSS TT+SA IR CSNQKLNLI+NGFHDYSSPARFPHL +KAAKNDRN
Sbjct: 1   MASSSFSPSANCFSSTTTLSAIIRDCSNQKLNLIHNGFHDYSSPARFPHLILKAAKNDRN 60

Query: 61  TKPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           TKPNSVICGDCDGNGAV+CSQCKG GVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  TKPNSVICGDCDGNGAVVCSQCKGKGVNAVDFFNGQFKAGESCWLCG 107

[Close]

BLAST of Lsi04G021360 vs. ExPASy TrEMBL
Match: A0A6J1D4M9 (uncharacterized protein LOC111016947 OS=Momordica charantia OX=3673 GN=LOC111016947 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.5e-39
Identity = 86/106 (81.13%), Postives = 91/106 (85.85%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNT 60
           MA S+FSASA CF+S TTV AI GCSN KLNLI+NG H YS  ARFPHLN KAAKNDRNT
Sbjct: 1   MAFSAFSASAGCFNS-TTVKAIGGCSNHKLNLIDNGLH-YSPAARFPHLNGKAAKNDRNT 60

Query: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           KPNS+ICGDCDGNGAVLCSQCKG+GVN  D FNGQFKAG SCWLCG
Sbjct: 61  KPNSMICGDCDGNGAVLCSQCKGSGVNPADVFNGQFKAGESCWLCG 104

[Close]

BLAST of Lsi04G021360 vs. NCBI nr
Match: XP_038899028.1 (protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 211.8 bits (538), Expect = 2.8e-51
Identity = 100/106 (94.34%), Postives = 104/106 (98.11%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNT 60
           MASSSFSASANCFSS TT+SAIRG SNQKLNL+NNGF+DYSSPARFPHLN+KAAKNDRNT
Sbjct: 1   MASSSFSASANCFSSSTTISAIRGSSNQKLNLVNNGFYDYSSPARFPHLNIKAAKNDRNT 60

Query: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG
Sbjct: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 106

[Close]

BLAST of Lsi04G021360 vs. NCBI nr
Match: XP_008466412.1 (PREDICTED: chaperone protein DnaJ-like [Cucumis melo] >TYK31430.1 chaperone protein DnaJ-like [Cucumis melo var. makuwa])

HSP 1 Score: 204.1 bits (518), Expect = 5.8e-49
Identity = 97/106 (91.51%), Postives = 101/106 (95.28%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNT 60
           MASSSFS SANCFSS TT+SA+R CSNQKLNLI+NGFHDYSSPARFPHL +KAAKNDRNT
Sbjct: 1   MASSSFSLSANCFSSTTTLSALRDCSNQKLNLIHNGFHDYSSPARFPHLILKAAKNDRNT 60

Query: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGKSCWLCG 106

[Close]

BLAST of Lsi04G021360 vs. NCBI nr
Match: KAA0038807.1 (chaperone protein DnaJ-like [Cucumis melo var. makuwa])

HSP 1 Score: 202.6 bits (514), Expect = 1.7e-48
Identity = 97/106 (91.51%), Postives = 100/106 (94.34%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSAIRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRNT 60
           MASSSFS SANCFSS TT+SAIR CSNQKLNLI+N FHDYSSPARFPHL +KAAKNDRNT
Sbjct: 1   MASSSFSLSANCFSSTTTLSAIRDCSNQKLNLIHNAFHDYSSPARFPHLILKAAKNDRNT 60

Query: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  KPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGKSCWLCG 106

[Close]

BLAST of Lsi04G021360 vs. NCBI nr
Match: XP_031737825.1 (uncharacterized protein LOC101218754 isoform X1 [Cucumis sativus])

HSP 1 Score: 196.8 bits (499), Expect = 9.3e-47
Identity = 96/107 (89.72%), Postives = 100/107 (93.46%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSA-IRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRN 60
           MASSSFS SANCFSS TT+SA IR CSNQKLNLI+NGFHDYSSPARFPHL +KAAKNDRN
Sbjct: 1   MASSSFSPSANCFSSTTTLSAIIRDCSNQKLNLIHNGFHDYSSPARFPHLILKAAKNDRN 60

Query: 61  TKPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           TKPNSVICGDCDGNGAV+CSQCKG GVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  TKPNSVICGDCDGNGAVVCSQCKGKGVNAVDFFNGQFKAGESCWLCG 107

[Close]

BLAST of Lsi04G021360 vs. NCBI nr
Match: XP_004136477.1 (protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic isoform X2 [Cucumis sativus] >KGN60105.1 hypothetical protein Csa_001346 [Cucumis sativus])

HSP 1 Score: 196.8 bits (499), Expect = 9.3e-47
Identity = 96/107 (89.72%), Postives = 100/107 (93.46%), Query Frame = 0


Query: 1   MASSSFSASANCFSSRTTVSA-IRGCSNQKLNLINNGFHDYSSPARFPHLNVKAAKNDRN 60
           MASSSFS SANCFSS TT+SA IR CSNQKLNLI+NGFHDYSSPARFPHL +KAAKNDRN
Sbjct: 1   MASSSFSPSANCFSSTTTLSAIIRDCSNQKLNLIHNGFHDYSSPARFPHLILKAAKNDRN 60

Query: 61  TKPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASCWLCG 107
           TKPNSVICGDCDGNGAV+CSQCKG GVNAVDFFNGQFKAG SCWLCG
Sbjct: 61  TKPNSVICGDCDGNGAVVCSQCKGKGVNAVDFFNGQFKAGESCWLCG 107

[Close]

BLAST of Lsi04G021360 vs. TAIR 10
Match: AT3G47650.1 (DnaJ/Hsp40 cysteine-rich domain superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 1.1e-18
Identity = 39/63 (61.90%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 44  ARFPHLNVKAAKND-RNTKPNSVICGDCDGNGAVLCSQCKGNGVNAVDFFNGQFKAGASC 103
           +R     VKAA N+ + TKPNS++C +C+G G V CSQCKG GVN +D FNGQFKAGA C
Sbjct: 48  SRSQSFEVKAANNNPQGTKPNSLVCANCEGEGCVACSQCKGGGVNLIDHFNGQFKAGALC 107

Query: 104 WLC 106
           WLC
Sbjct: 108 WLC 110

The following BLAST results are available for this feature:

BLAST of Lsi04G021360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SN73	1.6e-17	61.90	Protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q9XF14	2.6e-15	52.46	Protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic OS=Zea mays OX=4577 GN=BSD2 PE=...	[more]

BLAST of Lsi04G021360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E725	2.8e-49	91.51	Chaperone protein DnaJ-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3CSH4	2.8e-49	91.51	chaperone protein DnaJ-like OS=Cucumis melo OX=3656 GN=LOC103503827 PE=4 SV=1	[more]
A0A5A7TB44	8.2e-49	91.51	Chaperone protein DnaJ-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A0A0LDP8	4.5e-47	89.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G878740 PE=4 SV=1	[more]
A0A6J1D4M9	1.5e-39	81.13	uncharacterized protein LOC111016947 OS=Momordica charantia OX=3673 GN=LOC111016...	[more]

BLAST of Lsi04G021360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899028.1	2.8e-51	94.34	protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic-like [Benincasa hispida]	[more]
XP_008466412.1	5.8e-49	91.51	PREDICTED: chaperone protein DnaJ-like [Cucumis melo] >TYK31430.1 chaperone prot...	[more]
KAA0038807.1	1.7e-48	91.51	chaperone protein DnaJ-like [Cucumis melo var. makuwa]	[more]
XP_031737825.1	9.3e-47	89.72	uncharacterized protein LOC101218754 isoform X1 [Cucumis sativus]	[more]
XP_004136477.1	9.3e-47	89.72	protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic isoform X2 [Cucumis sativus] >K...	[more]

BLAST of Lsi04G021360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47650.1	1.1e-18	61.90	DnaJ/Hsp40 cysteine-rich domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR15852	PLASTID TRANSCRIPTIONALLY ACTIVE PROTEIN	coord: 2..106
None	No IPR available	PANTHER	PTHR15852:SF51	PROTEIN BUNDLE SHEATH DEFECTIVE 2, CHLOROPLASTIC	coord: 2..106
IPR036410	Heat shock protein DnaJ, cysteine-rich domain superfamily	SUPERFAMILY	57938	DnaJ/Hsp40 cysteine-rich domain	coord: 62..106

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G021360.1	Lsi04G021360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0101031	chaperone complex
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0044183	protein folding chaperone