Lsi04G021300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G021300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein WVD2-like 5
Locationchr04: 28429360 .. 28433630 (+)
RNA-Seq ExpressionLsi04G021300
SyntenyLsi04G021300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTACTCCATAAATAATTTAAGAAACAAATAAAATTATATTTTCCATTAAAATTCTGTGTCTTCTTCATCTCTTTATTTTTTTTCTTCTCTCTCTCTTTCTTCTTTGATAGAAAGCGGACTTGACCTGGAAATGGATTTGAATGCTACTGCTCTCTCCACTCCCCGCTTCTGAGATTTCCATTTCCCTCTCATTTTTCTCTTTCAATTCCCTTTCAATCTTCTCCAATTTGTTCTTCCATTGTTGGAATCGCTAGATCTTGTTTCGGATCCGTGGATCTCTGCTCCGGCATTATCTCTTTCTCTTTTGTTGCAGATCTTTTCCGCTGTTTCTTCAATCTCGCGAACTCAAATCCAAGCTTACGCGCTTTTGTTTTTCATGAGGTTCTGATCGTTTGCGCGGTTTTCGAGGTGAGTGAGTGAGTGTGTTGGTGTGGTTTTGTTTCTTTATGAGCTTGCTGTGATGGAGGAGGAGGAGGAAGAGATGGAATGGTTGATTGTGGTGATGAATCTTATCGTTTTTGTGTGATTGTGTTTGTGAGAGGGAAGTTTAGTGTATGTTTTTTGTGGTTTCTTTCTTCCTTTTTATTGTGGTAGAGCAGAAAGGTAGATGTCTTTTTTGTGTGTGTTTGGTTTATTCTGAGATGGATTGATTTGCCGCATGCTTATTCTACGAATAGATCGAGTTAGAATAAGGTTTTTCATTTGATATATATGCTTGAATCTGGATAATGGAATGAGAATGGTGGAATTTTGATTTTGGCATATGTTGCCTCAATCTGCAAATCGGAGGTGTGTGTGACGATGCTTGGATTTCTTGATTTCGCTTGGTTGTTTGGTGACGGAATTTCTGTAATGTGATTGATTGATGAGTGAGTGTGGCTAATTAATAGTTTCGGTTTATGGTTGAAAGACGAAGTCGTCGGAAGGTTATTTAAATCATTGCCTATGTCTTTCTTCACGGTTTCTTTGGGAAGAAATGGTTGACGGTTTTCAAACCATCCTCTACCACGTGTCCTAGTTCTAGAAATGTGTTGTTCTTTAAATTATTTTGGAACTAACTTGCTGTTAATAAATTCTTTTTTTTCGGACGTGCTTTTCGGTTGTCGTATGAATCGTGAAGACTGTGAAATGGGTGAATAATTTTCTCTGTTTGTCTTTCAGTTGATAATTTCTGTAAGTTTGATGTTAATGTATGGCATCGACCTGATGGATGGCAAATTTTTTGCAATTGAGAATTCTCTTATGTGTTATTAGACTGTTAATTACGACGTAATTCCAATTTATGTTACGGAGGATATATCAGAAAATTATTTCCTTGGTCGTAATGGTTTTGGTTGAAAACTTTTGCAATTCTACTTGATGTGGTTTGATATCTTTGTAATGCTTGTTTGATCTGTTGTAGTTTACACCAAATGATGGAATCGGAAATCCTTGTACCTGCTGATGGGCTCAAACTGACCCTTCAAAATGGGTTTCATGAGCATGTTTCTGCCTCCCCAGAGGAAATTGTTCCTAAGGTGATTGTGAGTGAGGGCATAAACAAAGACACAGGAGCTACAATGCAGCAAGAAAATATCGAGAATGATATCAAATTGAGTGCCAGTGCAACCAACGAATTGACCACAAGAGAACTCACGGAGGGATCAAGCTTTCCGGCAGAAAGCGATATATCGACCCTTTCTAAGGCAGCCTACTTTGTTTAATCTGTTCCTTTATATGAAGTAGTTTATCTTATTTGACTCTAACAGGTGCAGTTTCTTCTTTGATTGCATTCTTTTTAGGAAGGAGAGGAAAAGAATGTTGATCCTCCCAAGAAAATGAAACCAGAGAAGGGTCAAGTTAAGAGCAAGAATGAAAAGTCATCAGGCTTTAAACACATTTCTTCGACTGGGGTGAATAAGAATAAAGATGGAAAAGATGCAGAACAGATTGCTGCTCTTTTAAATGATTCAGGTACAGGTACATCGCATCCACACTCAAAACGGCCTAGTAAGAGTAGATCATTCAATGAGAGGCAAACTCAAGTGCCAAAGGTAATAGTTTCTGCACATTGCCTTCTTGACTCGTCTATGCTCTCGAATACTCATCCCCCTGGGCGGGTTGCAGTTTTGATTATATCTTATTCTGCTCGATGATCCCCTTCTCATATTCTTTTTGTCTGTTTGAAGCAGCAACCTGAGAAATCTGATGGGGAAGGCTCCAAGTATGTTCTGCCTGCTTTTATGATTTTATTGACTCTTCTTGGTTGGTTTAAATATTTTTTTAGTTTTATATCTAATTGGAGATCTATTTTTGCGTACATAATGAATGGAGAGAGCTTTAACTTTCCACTAAATTATTGAAAGTGAATATCAATTAATACTCCAGAGATCACTAAGTTGAGACCCACTTTGTGCAGGGAGAATACGAACCTGAAACCTTTGAAGAAAGGTCAGCCTAGTAAATCAGAAGGAGAGTCGGAATCTTCTTTGTATCCTTTGTTTCTTACCTACTATTACATAGAATTTCCTGAAATATAAATAAACAAAAAATGTAGTAATATTTAGACATTGCTTCACGTTATCTTGTTTGTTGGATCCAAGAGTTTTATCCTTGAATTGTTAATAGAAGTCCCAGAGCAGGAGATGACAAACCAAATAGGGTTGGGAGACTTCCTAACTATGGTTTTAGTTTTAGATGCAATGAGCGTGCTGAGAAAAGAAGAGAGGTCTGTTTTTGGATACATTTTCGTGCACTTTTTATTTATTTATTATTTTTTACTGCTACTCTTCTCCAACGATTTCTGTGTTGAAATTTTTATCACCATCATTATTTTTATATATAATGTGGGGCAGTTCTATTCGAAACTTGAGGAAAAGATACAAGCTAAGGAAGTAGAAAAGAACACTTTACAGGCAAAGTCCAAGGTATCACCGTCTCTATTGTCACCTTAATTTGTTCGACATTTGGTTCATATATTATGAAATAGTCGCTCTGTTACAGGAAACTCAAGAGGCTGAGATTAAAATGCTTAGAAAGAGTTTGAACTTTAAAGCAACTCCCATGCCGAGCTTTTATCAGGAACCACCTCCTCCCAAAGTCGAATTGAAAAAGGTAACAACATTTGAAAAGGAGATTTGAGTTTGGAATTCATTAATATCGGTTTCAAAATTGATTGGTTCTCTCATGAACTTGTGGAGAGACAATTTCTTGTATTTTTGCTGCTCAGTCTGAATTATAATTATGGCTCAAGAAGACACTAGGTGAATTTACCGCTGTCCTTTTATAAAATATCTACTATTGTGTGTGGACCCTGTAGGTATTTTGCATTCTAGCTGTCAATCATCTGCATATCATGTAACCGAGATTCTTAGGTGACAGTTTTAGATTCAATGATTTTAGTTCCGTGGCATTTGTGAAGATTTCTTGTAGCATAATGCTTAGAAGCAAACTTGGGCATTTTGATCCTTTTTTTATTAAGTTGATTTAAGGTCTGTTTGGATTGACTAAAGAAAAAAATATTTTTCGAAAAAGTCATTTTTATTTAAACTCTTTTGATAAAATTTGTTTAAAATACACTTTAAAAGTGTTTCAAAAACTATTTTGAGTGGTTACCAAATATTTCAATTTTTTCAAAATGACTTATTTTCAAAATAAAACACTTTAAAAGTTAAACCAAACACACCCTTAAAGTTGACTAATGACAACAACAAATTGTTGATGTAGATACCACCTACAAGAGCCAAGTCTCCCAAGCTTGGCCGAAAGAAGAGTTCAACCCCAGCAGCAGACTCTTCAAGTAATGATGGCGGTGATGTGCGATCAGCTCGCTTAAGTCTCGACGAGAATGTGGCTCTGAATAACAATTCTAAGAGGGTCTCTCCTGCCCGTTCAGACAAGCCGAAACGCAGGTCTCTTCCTAGTCTGCCTTCTGAGAAAACTACGATACCTGGTGTCGTAACAAATGCAGGGAAATCCTCTGCAACCAAGGTGAAAAATGTGGAGAAGCCTGCAGCAGCATCTACTACAAATGGAAAGAAAGAAGAAAAACGGGCATCATCTGAAGCAGCAACAACAAAGAAGTCTGCGTGTTTAAGATCGATAGACGAAGAGAAAACTGCTCCATCAAACACAACAAACAAAGTCGCGAATCTGAGCCAGGAGGAGAATGGAGTTGAGGCTAGTGAAACAGAGAACCAGACAGACGATGAACCAGAGAATGAAGAACAAGATGAGGATCAGAGCTTGGTTGAAGACGGTGCAGAATTGGAGCAGTCATCGAGCATCGTT

mRNA sequence

ATCTACTCCATAAATAATTTAAGAAACAAATAAAATTATATTTTCCATTAAAATTCTGTGTCTTCTTCATCTCTTTATTTTTTTTCTTCTCTCTCTCTTTCTTCTTTGATAGAAAGCGGACTTGACCTGGAAATGGATTTGAATGCTACTGCTCTCTCCACTCCCCGCTTCTGAGATTTCCATTTCCCTCTCATTTTTCTCTTTCAATTCCCTTTCAATCTTCTCCAATTTGTTCTTCCATTGTTGGAATCGCTAGATCTTGTTTCGGATCCGTGGATCTCTGCTCCGGCATTATCTCTTTCTCTTTTGTTGCAGATCTTTTCCGCTGTTTCTTCAATCTCGCGAACTCAAATCCAAGCTTACGCGCTTTTGTTTTTCATGAGGTTCTGATCGTTTGCGCGGTTTTCGAGCTTGCTGTGATGGAGGAGGAGGAGGAAGAGATGGAATGGTTGATTGTGGTGATGAATCTTATCTTGATAATTTCTGTAAGTTTGATGTTAATGTATGGCATCGACCTGATGGATGGCAAATTTTTTGCAATTGAGAATTCTCTTATTTTACACCAAATGATGGAATCGGAAATCCTTGTACCTGCTGATGGGCTCAAACTGACCCTTCAAAATGGGTTTCATGAGCATGTTTCTGCCTCCCCAGAGGAAATTGTTCCTAAGGTGATTGTGAGTGAGGGCATAAACAAAGACACAGGAGCTACAATGCAGCAAGAAAATATCGAGAATGATATCAAATTGAGTGCCAGTGCAACCAACGAATTGACCACAAGAGAACTCACGGAGGGATCAAGCTTTCCGGCAGAAAGCGATATATCGACCCTTTCTAAGGCAGCCTACTTTGAAGGAGAGGAAAAGAATGTTGATCCTCCCAAGAAAATGAAACCAGAGAAGGGTCAAGTTAAGAGCAAGAATGAAAAGTCATCAGGCTTTAAACACATTTCTTCGACTGGGGTGAATAAGAATAAAGATGGAAAAGATGCAGAACAGATTGCTGCTCTTTTAAATGATTCAGGTACAGGTACATCGCATCCACACTCAAAACGGCCTAGTAAGAGTAGATCATTCAATGAGAGGCAAACTCAAGTGCCAAAGGTAATAGTTTCTGCACATTGCCTTCTTGACTCGTCTATGCTCTCGAATACTCATCCCCCTGGGCGGCAGCAACCTGAGAAATCTGATGGGGAAGGCTCCAAGGAGAATACGAACCTGAAACCTTTGAAGAAAGGTCAGCCTAGTAAATCAGAAGGAGAGTCGGAATCTTCTTTAAGTCCCAGAGCAGGAGATGACAAACCAAATAGGGTTGGGAGACTTCCTAACTATGGTTTTAGTTTTAGATGCAATGAGCGTGCTGAGAAAAGAAGAGAGTTCTATTCGAAACTTGAGGAAAAGATACAAGCTAAGGAAGTAGAAAAGAACACTTTACAGGCAAAGTCCAAGGAAACTCAAGAGGCTGAGATTAAAATGCTTAGAAAGAGTTTGAACTTTAAAGCAACTCCCATGCCGAGCTTTTATCAGGAACCACCTCCTCCCAAAGTCGAATTGAAAAAGAGTTCAACCCCAGCAGCAGACTCTTCAAGTAATGATGGCGGTGATGTGCGATCAGCTCGCTTAAGTCTCGACGAGAATGTGGCTCTGAATAACAATTCTAAGAGGGTCTCTCCTGCCCGTTCAGACAAGCCGAAACGCAGGTCTCTTCCTAGTCTGCCTTCTGAGAAAACTACGATACCTGGTGTCGTAACAAATGCAGGGAAATCCTCTGCAACCAAGGTGAAAAATGTGGAGAAGCCTGCAGCAGCATCTACTACAAATGGAAAGAAAGAAGAAAAACGGGCATCATCTGAAGCAGCAACAACAAAGAAGTCTGCGTGTTTAAGATCGATAGACGAAGAGAAAACTGCTCCATCAAACACAACAAACAAAGTCGCGAATCTGAGCCAGGAGGAGAATGGAGTTGAGGCTAGTGAAACAGAGAACCAGACAGACGATGAACCAGAGAATGAAGAACAAGATGAGGATCAGAGCTTGGTTGAAGACGGTGCAGAATTGGAGCAGTCATCGAGCATCGTT

Coding sequence (CDS)

ATGGAGGAGGAGGAGGAAGAGATGGAATGGTTGATTGTGGTGATGAATCTTATCTTGATAATTTCTGTAAGTTTGATGTTAATGTATGGCATCGACCTGATGGATGGCAAATTTTTTGCAATTGAGAATTCTCTTATTTTACACCAAATGATGGAATCGGAAATCCTTGTACCTGCTGATGGGCTCAAACTGACCCTTCAAAATGGGTTTCATGAGCATGTTTCTGCCTCCCCAGAGGAAATTGTTCCTAAGGTGATTGTGAGTGAGGGCATAAACAAAGACACAGGAGCTACAATGCAGCAAGAAAATATCGAGAATGATATCAAATTGAGTGCCAGTGCAACCAACGAATTGACCACAAGAGAACTCACGGAGGGATCAAGCTTTCCGGCAGAAAGCGATATATCGACCCTTTCTAAGGCAGCCTACTTTGAAGGAGAGGAAAAGAATGTTGATCCTCCCAAGAAAATGAAACCAGAGAAGGGTCAAGTTAAGAGCAAGAATGAAAAGTCATCAGGCTTTAAACACATTTCTTCGACTGGGGTGAATAAGAATAAAGATGGAAAAGATGCAGAACAGATTGCTGCTCTTTTAAATGATTCAGGTACAGGTACATCGCATCCACACTCAAAACGGCCTAGTAAGAGTAGATCATTCAATGAGAGGCAAACTCAAGTGCCAAAGGTAATAGTTTCTGCACATTGCCTTCTTGACTCGTCTATGCTCTCGAATACTCATCCCCCTGGGCGGCAGCAACCTGAGAAATCTGATGGGGAAGGCTCCAAGGAGAATACGAACCTGAAACCTTTGAAGAAAGGTCAGCCTAGTAAATCAGAAGGAGAGTCGGAATCTTCTTTAAGTCCCAGAGCAGGAGATGACAAACCAAATAGGGTTGGGAGACTTCCTAACTATGGTTTTAGTTTTAGATGCAATGAGCGTGCTGAGAAAAGAAGAGAGTTCTATTCGAAACTTGAGGAAAAGATACAAGCTAAGGAAGTAGAAAAGAACACTTTACAGGCAAAGTCCAAGGAAACTCAAGAGGCTGAGATTAAAATGCTTAGAAAGAGTTTGAACTTTAAAGCAACTCCCATGCCGAGCTTTTATCAGGAACCACCTCCTCCCAAAGTCGAATTGAAAAAGAGTTCAACCCCAGCAGCAGACTCTTCAAGTAATGATGGCGGTGATGTGCGATCAGCTCGCTTAAGTCTCGACGAGAATGTGGCTCTGAATAACAATTCTAAGAGGGTCTCTCCTGCCCGTTCAGACAAGCCGAAACGCAGGTCTCTTCCTAGTCTGCCTTCTGAGAAAACTACGATACCTGGTGTCGTAACAAATGCAGGGAAATCCTCTGCAACCAAGGTGAAAAATGTGGAGAAGCCTGCAGCAGCATCTACTACAAATGGAAAGAAAGAAGAAAAACGGGCATCATCTGAAGCAGCAACAACAAAGAAGTCTGCGTGTTTAAGATCGATAGACGAAGAGAAAACTGCTCCATCAAACACAACAAACAAAGTCGCGAATCTGAGCCAGGAGGAGAATGGAGTTGAGGCTAGTGAAACAGAGAACCAGACAGACGATGAACCAGAGAATGAAGAACAAGATGAGGATCAGAGCTTGGTTGAAGACGGTGCAGAATTGGAGCAGTCATCGAGCATCGTT

Protein sequence

MEEEEEEMEWLIVVMNLILIISVSLMLMYGIDLMDGKFFAIENSLILHQMMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIKLSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNEKSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKVIVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPRAGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPPPPKVELKKSSTPAADSSSNDGGDVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNAGKSSATKVKNVEKPAAASTTNGKKEEKRASSEAATTKKSACLRSIDEEKTAPSNTTNKVANLSQEENGVEASETENQTDDEPENEEQDEDQSLVEDGAELEQSSSIV
Homology
BLAST of Lsi04G021300 vs. ExPASy Swiss-Prot
Match: Q94C48 (Protein WVD2-like 5 OS=Arabidopsis thaliana OX=3702 GN=WDL5 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 6.6e-46
Identity = 167/428 (39.02%), Postives = 225/428 (52.57%), Query Frame = 0

Query: 115 TNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNEKSSGF 174
           T + T+    E S+  +  D     K A    + ++VD  K MK EK Q K ++EK SG 
Sbjct: 38  TVDTTSESQNENSANSSTLDTIEHVKEAAEGTQVEHVDDSKCMKGEKAQRKPRHEKLSGG 97

Query: 175 KHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKVIVSAH 234
           K+ SS  + K+K+GK A+   A  N  G+   +  +  P KS+SFN R+ QV K      
Sbjct: 98  KNNSSVHIKKSKEGKSADAKVAASN--GSVAPNVQTTNPLKSKSFNGREAQVTK------ 157

Query: 235 CLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPRAGDDK 294
                      H       E +DGE  K     K  KK     SE +++SS SP+A D K
Sbjct: 158 --------QGKHDSA--PAESADGEKVKP----KSQKKQAHETSEDDTQSSNSPKADDGK 217

Query: 295 PNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMLR 354
           P +VG LPNYGFSF+C++RAEKR+EFY KLEEK  AKE E N++QAKSKETQEAE++MLR
Sbjct: 218 PRKVGALPNYGFSFKCDQRAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLR 277

Query: 355 KSLNFKATPMPSFYQEPPPPKVELKK------------SSTPAADSSSNDGGDVRSARLS 414
           KSLNFKATPMPSFYQEP PPK ELKK                A+ + S +    R  RLS
Sbjct: 278 KSLNFKATPMPSFYQEPQPPKTELKKIPPTRPKSPKLGRKKTASGADSEETQTPRLGRLS 337

Query: 415 LDENVALNN-NSKRVSPA--RSDKPKRRSLPSLPSEKTTIPGVVTNAGKSSATKVKNVEK 474
           LDE  + +N  +K + P      +P R+SLP LPS+KT +P      GK +  K      
Sbjct: 338 LDERASKDNPTAKGIMPTVDLKKQPVRKSLPRLPSQKTVLPD-----GKPAPAK------ 397

Query: 475 PAAASTTNGKKEEKRASSEAATTKKSACLRSIDEEKTAPSN--------TTNKVANLSQE 520
            AA      + E+K+   +A T  +++     + + T  SN        T +   N  + 
Sbjct: 398 -AAIIPAKVRPEKKKLEKDAETVNQTSHPTEEEAQVTVSSNADVEDSHETVSPRMNEDRA 431

BLAST of Lsi04G021300 vs. ExPASy Swiss-Prot
Match: Q0WSZ8 (Protein WVD2-like 6 OS=Arabidopsis thaliana OX=3702 GN=WDL6 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 4.6e-39
Identity = 145/384 (37.76%), Postives = 200/384 (52.08%), Query Frame = 0

Query: 147 EEKNVDPPKKMKPEKGQVKSKNEKSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTS 206
           + + VD  K+M+ +K Q + K+EK+SG K+I S  V K KDGK       ++  +G+   
Sbjct: 54  QNEQVDDSKQMRGQKVQGRVKHEKTSGGKNIPSVLVKKKKDGK-------VVASNGSVAP 113

Query: 207 HPHSKRPSKSRSFNERQTQVPKVIVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTN 266
           +    +  KS+S N R+  V K                         +    EG+++   
Sbjct: 114 NVKPVKSPKSKSLNGREAHVTK---------------------HGNHDSLPAEGTRDKPK 173

Query: 267 LKPLKKGQPSKSEGESESSLSPRAGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEE 326
           L+  +K     SE +++    P+  D KP R   LPNYGFSFRC++RAEKRREFYSKLEE
Sbjct: 174 LRETRKQVNDTSEDDTQ---YPKEDDGKPRRASALPNYGFSFRCDQRAEKRREFYSKLEE 233

Query: 327 KIQAKEVEKNTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPPPPKVELKK------ 386
           KI AKE EKNT+QAKSKETQEAE+KMLRKSLNFKATPMP+FYQEP  PK ELKK      
Sbjct: 234 KIHAKEEEKNTVQAKSKETQEAELKMLRKSLNFKATPMPTFYQEPQLPKTELKKIAITRP 293

Query: 387 SSTPAADSSSNDGGDVRSA---------RLSLDENVALNNNSKRVS-PARSDKPK-RRSL 446
            S       +N   D   A         RLSLDE    +N     S P  + KP  R+SL
Sbjct: 294 KSPKLGRKKTNSRADSEEAITIQTPRFGRLSLDEKTPKDNPVVEGSVPGETKKPPVRKSL 353

Query: 447 PSLPSEKTTIPGVVTNAGKSSATKVKNVEKPAAASTTNGKKEEKRASSEAATTKKSACLR 506
           P LPSEKT +     + GK +  K       A  ++T  K E K+   +     +S+ + 
Sbjct: 354 PRLPSEKTNL-----SNGKVAPAK-------AVTASTKAKSERKKPDKDVDDLSQSSPV- 390

Query: 507 SIDEEKTAPSNTTNKVANLSQEEN 514
              ++   P ++  +   ++++ N
Sbjct: 414 ---DDNADPEDSQEQAPRVNEDRN 390

BLAST of Lsi04G021300 vs. ExPASy Swiss-Prot
Match: Q9SJ62 (Protein WVD2-like 4 OS=Arabidopsis thaliana OX=3702 GN=WDL4 PE=1 SV=2)

HSP 1 Score: 101.3 bits (251), Expect = 3.6e-20
Identity = 91/213 (42.72%), Postives = 115/213 (53.99%), Query Frame = 0

Query: 269 PLKKGQPSKSEGESES-SLSPRAGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEK 328
           PLK    SK + ++ S + S      + + VG     GFSFR  ERAEKR+EFY KLEEK
Sbjct: 210 PLKAEMASKDDEDTRSTTTSTSTPRGRRSSVGSAS--GFSFRLEERAEKRKEFYMKLEEK 269

Query: 329 IQAKEVEKNTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPPPPKVELKKSSTPAAD 388
           I AKEVEK  LQAKSKE+QE EIK LRKSL FKA PMPSFY+E PPPKVELKK  T    
Sbjct: 270 IHAKEVEKTNLQAKSKESQEEEIKRLRKSLTFKAGPMPSFYKE-PPPKVELKKIPT-TRP 329

Query: 389 SSSNDGGDVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNAG 448
            S   G    S+  +  E        K  S +   KP  +S P L +++ ++        
Sbjct: 330 KSPKLGRRKSSSDATGGEAAPRVTKPKDSSSSTVKKPITKSQPKLETQEKSV-------- 389

Query: 449 KSSATKVKNVEKPAAASTTNGKKEEKRASSEAA 481
                K K  +K         + EE++A++ AA
Sbjct: 390 -----KAKEKKKKVKKEEAEKRGEEEKATAVAA 405

BLAST of Lsi04G021300 vs. ExPASy Swiss-Prot
Match: Q67Y69 (Protein WVD2-like 7 OS=Arabidopsis thaliana OX=3702 GN=WDL7 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.4e-16
Identity = 77/214 (35.98%), Postives = 105/214 (49.07%), Query Frame = 0

Query: 193 QIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKVIVSAHCLLDSSMLSNTHPP---G 252
           Q +AL+ ++G+      SK+PS S         V  +I +   +  +S   +   P    
Sbjct: 200 QDSALVEEAGSKLDEHASKKPSNSMETPSSSVNVKPIIPNDVRVTKASTKGHDVTPKAAS 259

Query: 253 RQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPRAGDDKPNRVGRLP------- 312
           R+    S    SK N + K  K+ +P K+  ES+   S +     P    R         
Sbjct: 260 RRTKGSSLSSNSKTNVDAKSQKELRPKKTI-ESQPKSSNKTETRPPIATNRCKTSTTSSK 319

Query: 313 ------NYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMLRKS 372
                 +  F F+C+ERAEKR+EFY KLEEKI AK+ E N +QAK+++  EAEIK  RKS
Sbjct: 320 LEMSTGSTSFRFKCSERAEKRKEFYMKLEEKIHAKKTETNQVQAKTQQKAEAEIKQFRKS 379

Query: 373 LNFKATPMPSFYQEPPPPKVELKKSSTPAADSSS 391
           LNFKATPMPSFY     P    K   +  A S S
Sbjct: 380 LNFKATPMPSFYNIGTRPVSHNKTEPSKVAQSRS 412

BLAST of Lsi04G021300 vs. ExPASy Swiss-Prot
Match: Q84WL6 (Protein WVD2-like 3 OS=Arabidopsis thaliana OX=3702 GN=WDL3 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.6e-15
Identity = 85/234 (36.32%), Postives = 107/234 (45.73%), Query Frame = 0

Query: 158 KPEKGQVKSKNEKSSGF--KHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSK 217
           KP     +SK+   S    KH S +G   NK      Q  +L  +          KR S 
Sbjct: 72  KPIGDDFESKDVTKSSLHAKHASKSGRGNNKTRNTVPQPFSLATE----------KRASS 131

Query: 218 SRSFNERQTQVPKVIVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQP 277
           +RSF                 L+S+ L    P G      S  +        KPL+    
Sbjct: 132 TRSFTSES-------------LESAGLKK-FPDGH-----SKVQSQATKVPRKPLQPKNK 191

Query: 278 SKSEGESESSLSPRAGDDKPNRVGR-LPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVE 337
             S+ E   S++  A     +   R +     SFR  ERAEKR+EFY+KLEEK QA E E
Sbjct: 192 KLSDEEDSCSVASYATSGAKSAKSRTVVTAAPSFRSTERAEKRKEFYTKLEEKHQAMEAE 251

Query: 338 KNTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPPPPKVELKKSSTPAADS 389
           K   +A++KE  EA ++ LRKSL FKA PMP FY E PPPKVELKK     A S
Sbjct: 252 KTQSEARNKEATEAALRQLRKSLRFKANPMPKFYHEGPPPKVELKKPLPTRAKS 276

BLAST of Lsi04G021300 vs. ExPASy TrEMBL
Match: A0A5D3E7Z5 (Protein WVD2-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007460 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 9.5e-181
Identity = 398/540 (73.70%), Postives = 430/540 (79.63%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPK IVSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKAIVSEDIDKDTGSPMQQENIEDDI- 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
            S S TNE TTRELTEG +FP ESDIS L KA Y EGEEK+VDPPKK+KPEK Q+KSKNE
Sbjct: 61  -SGSTTNESTTRELTEGLNFPEESDISILPKACYLEGEEKSVDPPKKVKPEKAQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  KH+SSTG  KNKDGK+AE    LLN SGTG SH H K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKHVSSTGAKKNKDGKEAEH---LLNGSGTGVSHSHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                                 Q EKSDGEGSKENT+LKPLKKGQPSKSEGESESS+SPR
Sbjct: 181 ----------------------QTEKSDGEGSKENTSLKPLKKGQPSKSEGESESSISPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQEAE
Sbjct: 241 AGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPKVEL               KKSSTP ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTP-ADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSATK 469
           DVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK TIPGV+TNA GK+SATK
Sbjct: 361 DVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKITIPGVITNAGGKTSATK 420

Query: 470 VKNVEK------------PAAASTTNGKKEEKRASSEAA-----TTKKSACLRSIDEEKT 529
           VKNVEK             AA++TTNGKKEEKR SSEAA     +TKKSA LRS +EEKT
Sbjct: 421 VKNVEKEKEKEKEKEKPTSAASTTTNGKKEEKRTSSEAAAATTTSTKKSASLRSTNEEKT 480

Query: 530 APSNTTNKVANLS-QEENGVEASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           APS  +N+V N+S +EENGVE S+TENQ  TDDE ENEEQD+ Q+  E+G    QSS+I+
Sbjct: 481 APSIASNEVVNISREEENGVEPSKTENQNHTDDELENEEQDQQQNSEEEGT--VQSSNII 508

BLAST of Lsi04G021300 vs. ExPASy TrEMBL
Match: A0A1S3CR67 (protein WVD2-like 5 OS=Cucumis melo OX=3656 GN=LOC103503819 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 1.3e-177
Identity = 396/540 (73.33%), Postives = 428/540 (79.26%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPK IVSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKAIVSEDIDKDTGSPMQQENIEDDI- 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
            S S TNE TTRELTEG +FP ESDIS L K    EGEEK+VDPPKK+KPEK Q+KSKNE
Sbjct: 61  -SGSTTNESTTRELTEGLNFPEESDISILPK----EGEEKSVDPPKKVKPEKAQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  KH+SSTG  KNKDGK+AE    LLN SGTG SH H K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKHVSSTGAKKNKDGKEAEH---LLNGSGTGVSHSHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                                 Q EKSDGEGSKENT+LKPLKKGQPSKSEGESESS+SPR
Sbjct: 181 ----------------------QTEKSDGEGSKENTSLKPLKKGQPSKSEGESESSISPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQEAE
Sbjct: 241 AGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPKVEL               KKSSTP ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTP-ADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSATK 469
           DVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK TIPGV+TNA GK+SATK
Sbjct: 361 DVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKITIPGVITNAGGKTSATK 420

Query: 470 VKNVEK------------PAAASTTNGKKEEKRASSEAA-----TTKKSACLRSIDEEKT 529
           VKNVEK             AA++TTNGKKEEKR SSEAA     +TKKSA LRS +EEKT
Sbjct: 421 VKNVEKEKEKEKEKEKPTSAASTTTNGKKEEKRTSSEAAAATTTSTKKSASLRSTNEEKT 480

Query: 530 APSNTTNKVANLS-QEENGVEASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           APS  +N+V N+S +EENGVE S+TENQ  TDDE ENEEQD+ Q+  E+G    QSS+I+
Sbjct: 481 APSIASNEVVNISREEENGVEPSKTENQNHTDDELENEEQDQQQNSEEEGT--VQSSNII 504

BLAST of Lsi04G021300 vs. ExPASy TrEMBL
Match: A0A5A7T5Q5 (Protein WVD2-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G003690 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 6.3e-177
Identity = 395/540 (73.15%), Postives = 427/540 (79.07%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPK IVSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKAIVSEDIDKDTGSPMQQENIEDDI- 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
            S S TNE TTRELTEG +FP ESDIS L KA     EEK+VDPPKK+KPEK Q+KSKNE
Sbjct: 61  -SGSTTNESTTRELTEGLNFPEESDISILPKAC----EEKSVDPPKKVKPEKAQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  KH+SSTG  KNKDGK+AE    LLN SGTG SH H K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKHVSSTGAKKNKDGKEAEH---LLNGSGTGVSHSHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                                 Q EKSDGEGSKENT+LKPLKKGQPSKSEGESESS+SPR
Sbjct: 181 ----------------------QTEKSDGEGSKENTSLKPLKKGQPSKSEGESESSISPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQEAE
Sbjct: 241 AGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPKVEL               KKSSTP ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTP-ADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSATK 469
           DVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK TIPGV+TNA GK+SATK
Sbjct: 361 DVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKITIPGVITNAGGKTSATK 420

Query: 470 VKNVEK------------PAAASTTNGKKEEKRASSEAA-----TTKKSACLRSIDEEKT 529
           VKNVEK             AA++TTNGKKEEKR SSEAA     +TKKSA LRS +EEKT
Sbjct: 421 VKNVEKEKEKEKEKEKPTSAASTTTNGKKEEKRTSSEAAAATTTSTKKSASLRSTNEEKT 480

Query: 530 APSNTTNKVANLS-QEENGVEASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           APS  +N+V N+S +EENGVE S+TENQ  TDDE ENEEQD+ Q+  E+G    QSS+I+
Sbjct: 481 APSIASNEVVNISREEENGVEPSKTENQNHTDDELENEEQDQQQNSEEEGT--VQSSNII 504

BLAST of Lsi04G021300 vs. ExPASy TrEMBL
Match: A0A0A0LGM7 (TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G878190 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 4.1e-176
Identity = 401/540 (74.26%), Postives = 425/540 (78.70%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPKV VSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKVTVSEDIDKDTGSPMQQENIEDDIN 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
              SATNE TTRELTEGS+FP ESDISTLS     EGEEK  DPPKK+KPEKGQ+KSKNE
Sbjct: 61  -DGSATNESTTRELTEGSNFPEESDISTLS----MEGEEKCGDPPKKVKPEKGQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  K ISSTGV KNKDGK+AE    LLN SGTG SHPH K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKQISSTGVKKNKDGKEAEH---LLNGSGTGASHPHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKS--DGEGSKENTNLKPLKKGQPSKSEGESESSLS 289
                                 Q EKS  DGEGSKENTNLKPLKKGQPSKSEGESESSLS
Sbjct: 181 ----------------------QTEKSDGDGEGSKENTNLKPLKKGQPSKSEGESESSLS 240

Query: 290 PRAGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQE 349
           PRAGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQE
Sbjct: 241 PRAGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQE 300

Query: 350 AEIKMLRKSLNFKATPMPSFYQEPPPPKVELK--------------KSSTPAADSSSNDG 409
           AEIKMLRKSLNFKATPMPSFYQEPPPPKVELK              K S+  ADSSSNDG
Sbjct: 301 AEIKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTLADSSSNDG 360

Query: 410 GDVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSAT 469
           GDVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK  IPGVV NA GKSSAT
Sbjct: 361 GDVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKIVIPGVVANAGGKSSAT 420

Query: 470 KVKNV----EKPAAAS----TTNGKKEEKRASSEAA------TTKKSACLRSIDEEKTAP 529
           KVK V    EKPAAAS    TTNGKKEEKR SSEAA      +TKKSA LRS +EEKTAP
Sbjct: 421 KVKIVEKEKEKPAAASATSTTTNGKKEEKRTSSEAAAAAATTSTKKSASLRSTNEEKTAP 480

Query: 530 SNTTNKVANLS-QEENGVE--ASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           S  TN+V NLS +EENGVE  +SETENQ  TD+E ENEEQD+ Q   E+   ++ SS I+
Sbjct: 481 SIATNEVVNLSREEENGVEPSSSETENQNHTDEELENEEQDQQQQNSEEEGAVQSSSIII 508

BLAST of Lsi04G021300 vs. ExPASy TrEMBL
Match: A0A6J1C259 (protein WVD2-like 5 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111006712 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 2.0e-175
Identity = 382/527 (72.49%), Postives = 428/527 (81.21%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPA+GLKLTLQNGFHEHVS S E+IVPKVIVSEGIN+D GATM+QEN+EN +K
Sbjct: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
           LS SATNE TTREL EGS+FPAE++ISTLSK    EGEE  VDPPK++K EKGQ KSKNE
Sbjct: 61  LSGSATNESTTRELMEGSNFPAENNISTLSK----EGEENYVDPPKQVKSEKGQTKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSSG KHISSTGVNKNKDGKDAEQ AALLN  G+GTSHPHSK+PSKSRSFN+RQ Q+PK 
Sbjct: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLN--GSGTSHPHSKQPSKSRSFNDRQAQIPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                               +QQPEKSDGEGSKENTNLKPLKKG PSKSEGESESSLSPR
Sbjct: 181 --------------------QQQPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AG++KP+RVGRLPNYGFSFRCNERAEKR+EFYSKLEEKI AKEVEK+TLQAKSKETQEAE
Sbjct: 241 AGEEKPHRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPK EL               KK S   ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNN-SKRVSPARSDKPKRRSLPSLPSEKTTI-PGVVTNAGKSSAT 469
           DVRS RLSLDE  +LNNN SK VSPAR +KPKRRSLP LPSEKT I  G V +AGKSSA+
Sbjct: 361 DVRSGRLSLDEKASLNNNSSKGVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSAS 420

Query: 470 KVKNVEKPAAASTTNGKKEEKRASSEAATTKKSACLRSIDEEKTAPSNTTNK-VANLSQE 529
           KVK+ EK A   +TNGKKEEK+A S+ ATT+K+AC RS+DEEK A  + TN+ +A+LSQE
Sbjct: 421 KVKSAEKTA---STNGKKEEKQAPSD-ATTEKTACSRSVDEEKIASLDATNEALASLSQE 480

Query: 530 ENGV---EASETENQTDD----EPENEEQDEDQSLVEDGAELEQSSS 552
           E+GV   EASETENQ+D+    E E++EQ + ++LVE+G E+EQSSS
Sbjct: 481 EDGVETAEASETENQSDEEAVIEEEHDEQQQHKTLVEEGVEMEQSSS 496

BLAST of Lsi04G021300 vs. NCBI nr
Match: XP_038898383.1 (protein WVD2-like 5 [Benincasa hispida])

HSP 1 Score: 733.8 bits (1893), Expect = 1.1e-207
Identity = 433/522 (82.95%), Postives = 448/522 (85.82%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHE VS S EEIVP+VIVSEGI KDTG TMQQENIENDIK
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEPVSGSSEEIVPEVIVSEGITKDTGDTMQQENIENDIK 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
           LS SA NE TTRELTEGS F AESDISTLSK    EGEEKNVDPPKK+KPEKGQ+KSKNE
Sbjct: 61  LSGSAANESTTRELTEGSDFLAESDISTLSK----EGEEKNVDPPKKVKPEKGQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSSG KHISSTG+NKNKDGKDAEQ AALLN SGTGTSHPH K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSGPKHISSTGMNKNKDGKDAEQTAALLNGSGTGTSHPHPKQPSKSRSFNERQPQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                                 QPEKSDGEGSKENTNLKPLKKGQPSKSEGESESS+SPR
Sbjct: 181 ----------------------QPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSISPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQEAE
Sbjct: 241 AGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPKVEL               KKSSTP ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTP-ADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNAGKSSATKV 469
           DVRSARLSLDENVALNNNSK V PARS+KPKRRSLP+LPSE+TTIPGVVTNAGKSSATKV
Sbjct: 361 DVRSARLSLDENVALNNNSKGVYPARSEKPKRRSLPNLPSERTTIPGVVTNAGKSSATKV 420

Query: 470 KNVEKP---AAASTTNGKKEEKRASSEAATTKKSACLRSIDEEKTAPSNTTNKVANLSQE 529
           KNVEKP   AAASTTNGKKEEKR SS+AATTKKSA LRSI EEKTAPSN TN V NLSQE
Sbjct: 421 KNVEKPAAAAAASTTNGKKEEKRVSSDAATTKKSASLRSI-EEKTAPSNATNDVVNLSQE 480

Query: 530 ENGVEASETENQTDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           ENGVE SETENQTDDEPENEEQD+ QS  E+GAELEQSS+ V
Sbjct: 481 ENGVEPSETENQTDDEPENEEQDQHQSSAEEGAELEQSSNTV 493

BLAST of Lsi04G021300 vs. NCBI nr
Match: TYK31435.1 (protein WVD2-like 5 [Cucumis melo var. makuwa])

HSP 1 Score: 643.3 bits (1658), Expect = 2.0e-180
Identity = 398/540 (73.70%), Postives = 430/540 (79.63%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPK IVSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKAIVSEDIDKDTGSPMQQENIEDDI- 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
            S S TNE TTRELTEG +FP ESDIS L KA Y EGEEK+VDPPKK+KPEK Q+KSKNE
Sbjct: 61  -SGSTTNESTTRELTEGLNFPEESDISILPKACYLEGEEKSVDPPKKVKPEKAQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  KH+SSTG  KNKDGK+AE    LLN SGTG SH H K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKHVSSTGAKKNKDGKEAEH---LLNGSGTGVSHSHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                                 Q EKSDGEGSKENT+LKPLKKGQPSKSEGESESS+SPR
Sbjct: 181 ----------------------QTEKSDGEGSKENTSLKPLKKGQPSKSEGESESSISPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQEAE
Sbjct: 241 AGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPKVEL               KKSSTP ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTP-ADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSATK 469
           DVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK TIPGV+TNA GK+SATK
Sbjct: 361 DVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKITIPGVITNAGGKTSATK 420

Query: 470 VKNVEK------------PAAASTTNGKKEEKRASSEAA-----TTKKSACLRSIDEEKT 529
           VKNVEK             AA++TTNGKKEEKR SSEAA     +TKKSA LRS +EEKT
Sbjct: 421 VKNVEKEKEKEKEKEKPTSAASTTTNGKKEEKRTSSEAAAATTTSTKKSASLRSTNEEKT 480

Query: 530 APSNTTNKVANLS-QEENGVEASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           APS  +N+V N+S +EENGVE S+TENQ  TDDE ENEEQD+ Q+  E+G    QSS+I+
Sbjct: 481 APSIASNEVVNISREEENGVEPSKTENQNHTDDELENEEQDQQQNSEEEGT--VQSSNII 508

BLAST of Lsi04G021300 vs. NCBI nr
Match: XP_008466404.1 (PREDICTED: protein WVD2-like 5 [Cucumis melo])

HSP 1 Score: 632.9 bits (1631), Expect = 2.6e-177
Identity = 396/540 (73.33%), Postives = 428/540 (79.26%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPK IVSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKAIVSEDIDKDTGSPMQQENIEDDI- 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
            S S TNE TTRELTEG +FP ESDIS L K    EGEEK+VDPPKK+KPEK Q+KSKNE
Sbjct: 61  -SGSTTNESTTRELTEGLNFPEESDISILPK----EGEEKSVDPPKKVKPEKAQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  KH+SSTG  KNKDGK+AE    LLN SGTG SH H K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKHVSSTGAKKNKDGKEAEH---LLNGSGTGVSHSHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                                 Q EKSDGEGSKENT+LKPLKKGQPSKSEGESESS+SPR
Sbjct: 181 ----------------------QTEKSDGEGSKENTSLKPLKKGQPSKSEGESESSISPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQEAE
Sbjct: 241 AGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPKVEL               KKSSTP ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTP-ADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSATK 469
           DVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK TIPGV+TNA GK+SATK
Sbjct: 361 DVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKITIPGVITNAGGKTSATK 420

Query: 470 VKNVEK------------PAAASTTNGKKEEKRASSEAA-----TTKKSACLRSIDEEKT 529
           VKNVEK             AA++TTNGKKEEKR SSEAA     +TKKSA LRS +EEKT
Sbjct: 421 VKNVEKEKEKEKEKEKPTSAASTTTNGKKEEKRTSSEAAAATTTSTKKSASLRSTNEEKT 480

Query: 530 APSNTTNKVANLS-QEENGVEASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           APS  +N+V N+S +EENGVE S+TENQ  TDDE ENEEQD+ Q+  E+G    QSS+I+
Sbjct: 481 APSIASNEVVNISREEENGVEPSKTENQNHTDDELENEEQDQQQNSEEEGT--VQSSNII 504

BLAST of Lsi04G021300 vs. NCBI nr
Match: KAA0038812.1 (protein WVD2-like 5 [Cucumis melo var. makuwa])

HSP 1 Score: 630.6 bits (1625), Expect = 1.3e-176
Identity = 395/540 (73.15%), Postives = 427/540 (79.07%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPK IVSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKAIVSEDIDKDTGSPMQQENIEDDI- 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
            S S TNE TTRELTEG +FP ESDIS L KA     EEK+VDPPKK+KPEK Q+KSKNE
Sbjct: 61  -SGSTTNESTTRELTEGLNFPEESDISILPKAC----EEKSVDPPKKVKPEKAQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  KH+SSTG  KNKDGK+AE    LLN SGTG SH H K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKHVSSTGAKKNKDGKEAEH---LLNGSGTGVSHSHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPR 289
                                 Q EKSDGEGSKENT+LKPLKKGQPSKSEGESESS+SPR
Sbjct: 181 ----------------------QTEKSDGEGSKENTSLKPLKKGQPSKSEGESESSISPR 240

Query: 290 AGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 349
           AGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQEAE
Sbjct: 241 AGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAE 300

Query: 350 IKMLRKSLNFKATPMPSFYQEPPPPKVEL---------------KKSSTPAADSSSNDGG 409
           IKMLRKSLNFKATPMPSFYQEPPPPKVEL               KKSSTP ADSSSNDGG
Sbjct: 301 IKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTP-ADSSSNDGG 360

Query: 410 DVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSATK 469
           DVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK TIPGV+TNA GK+SATK
Sbjct: 361 DVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKITIPGVITNAGGKTSATK 420

Query: 470 VKNVEK------------PAAASTTNGKKEEKRASSEAA-----TTKKSACLRSIDEEKT 529
           VKNVEK             AA++TTNGKKEEKR SSEAA     +TKKSA LRS +EEKT
Sbjct: 421 VKNVEKEKEKEKEKEKPTSAASTTTNGKKEEKRTSSEAAAATTTSTKKSASLRSTNEEKT 480

Query: 530 APSNTTNKVANLS-QEENGVEASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           APS  +N+V N+S +EENGVE S+TENQ  TDDE ENEEQD+ Q+  E+G    QSS+I+
Sbjct: 481 APSIASNEVVNISREEENGVEPSKTENQNHTDDELENEEQDQQQNSEEEGT--VQSSNII 504

BLAST of Lsi04G021300 vs. NCBI nr
Match: XP_031738605.1 (protein WVD2-like 5 [Cucumis sativus] >KGN60099.1 hypothetical protein Csa_002661 [Cucumis sativus])

HSP 1 Score: 627.9 bits (1618), Expect = 8.5e-176
Identity = 401/540 (74.26%), Postives = 425/540 (78.70%), Query Frame = 0

Query: 50  MMESEILVPADGLKLTLQNGFHEHVSASPEEIVPKVIVSEGINKDTGATMQQENIENDIK 109
           MMESEILVPADGLKLTLQNGFHEHVSA+ EEIVPKV VSE I+KDTG+ MQQENIE+DI 
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEHVSAA-EEIVPKVTVSEDIDKDTGSPMQQENIEDDIN 60

Query: 110 LSASATNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNE 169
              SATNE TTRELTEGS+FP ESDISTLS     EGEEK  DPPKK+KPEKGQ+KSKNE
Sbjct: 61  -DGSATNESTTRELTEGSNFPEESDISTLS----MEGEEKCGDPPKKVKPEKGQIKSKNE 120

Query: 170 KSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKV 229
           KSS  K ISSTGV KNKDGK+AE    LLN SGTG SHPH K+PSKSRSFNERQ QVPK 
Sbjct: 121 KSSSLKQISSTGVKKNKDGKEAEH---LLNGSGTGASHPHPKQPSKSRSFNERQAQVPK- 180

Query: 230 IVSAHCLLDSSMLSNTHPPGRQQPEKS--DGEGSKENTNLKPLKKGQPSKSEGESESSLS 289
                                 Q EKS  DGEGSKENTNLKPLKKGQPSKSEGESESSLS
Sbjct: 181 ----------------------QTEKSDGDGEGSKENTNLKPLKKGQPSKSEGESESSLS 240

Query: 290 PRAGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQE 349
           PRAGD+KPNRVGRLPNYGFSFRCNERAEKR+EFYSKLEEKIQAKEVEKNTLQAKSKETQE
Sbjct: 241 PRAGDEKPNRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQE 300

Query: 350 AEIKMLRKSLNFKATPMPSFYQEPPPPKVELK--------------KSSTPAADSSSNDG 409
           AEIKMLRKSLNFKATPMPSFYQEPPPPKVELK              K S+  ADSSSNDG
Sbjct: 301 AEIKMLRKSLNFKATPMPSFYQEPPPPKVELKKIPPTRAKSPKLGRKKSSTLADSSSNDG 360

Query: 410 GDVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNA-GKSSAT 469
           GDVRSARLSLDENVALNNNSK V P RSDKPKRRSLP+LPSEK  IPGVV NA GKSSAT
Sbjct: 361 GDVRSARLSLDENVALNNNSKGVYPVRSDKPKRRSLPNLPSEKIVIPGVVANAGGKSSAT 420

Query: 470 KVKNV----EKPAAAS----TTNGKKEEKRASSEAA------TTKKSACLRSIDEEKTAP 529
           KVK V    EKPAAAS    TTNGKKEEKR SSEAA      +TKKSA LRS +EEKTAP
Sbjct: 421 KVKIVEKEKEKPAAASATSTTTNGKKEEKRTSSEAAAAAATTSTKKSASLRSTNEEKTAP 480

Query: 530 SNTTNKVANLS-QEENGVE--ASETENQ--TDDEPENEEQDEDQSLVEDGAELEQSSSIV 554
           S  TN+V NLS +EENGVE  +SETENQ  TD+E ENEEQD+ Q   E+   ++ SS I+
Sbjct: 481 SIATNEVVNLSREEENGVEPSSSETENQNHTDEELENEEQDQQQQNSEEEGAVQSSSIII 508

BLAST of Lsi04G021300 vs. TAIR 10
Match: AT4G32330.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 186.8 bits (473), Expect = 4.7e-47
Identity = 167/428 (39.02%), Postives = 225/428 (52.57%), Query Frame = 0

Query: 115 TNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNEKSSGF 174
           T + T+    E S+  +  D     K A    + ++VD  K MK EK Q K ++EK SG 
Sbjct: 38  TVDTTSESQNENSANSSTLDTIEHVKEAAEGTQVEHVDDSKCMKGEKAQRKPRHEKLSGG 97

Query: 175 KHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKVIVSAH 234
           K+ SS  + K+K+GK A+   A  N  G+   +  +  P KS+SFN R+ QV K      
Sbjct: 98  KNNSSVHIKKSKEGKSADAKVAASN--GSVAPNVQTTNPLKSKSFNGREAQVTK------ 157

Query: 235 CLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPRAGDDK 294
                      H       E +DGE  K     K  KK     SE +++SS SP+A D K
Sbjct: 158 --------QGKHDSA--PAESADGEKVKP----KSQKKQAHETSEDDTQSSNSPKADDGK 217

Query: 295 PNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMLR 354
           P +VG LPNYGFSF+C++RAEKR+EFY KLEEK  AKE E N++QAKSKETQEAE++MLR
Sbjct: 218 PRKVGALPNYGFSFKCDQRAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLR 277

Query: 355 KSLNFKATPMPSFYQEPPPPKVELKK------------SSTPAADSSSNDGGDVRSARLS 414
           KSLNFKATPMPSFYQEP PPK ELKK                A+ + S +    R  RLS
Sbjct: 278 KSLNFKATPMPSFYQEPQPPKTELKKIPPTRPKSPKLGRKKTASGADSEETQTPRLGRLS 337

Query: 415 LDENVALNN-NSKRVSPA--RSDKPKRRSLPSLPSEKTTIPGVVTNAGKSSATKVKNVEK 474
           LDE  + +N  +K + P      +P R+SLP LPS+KT +P      GK +  K      
Sbjct: 338 LDERASKDNPTAKGIMPTVDLKKQPVRKSLPRLPSQKTVLPD-----GKPAPAK------ 397

Query: 475 PAAASTTNGKKEEKRASSEAATTKKSACLRSIDEEKTAPSN--------TTNKVANLSQE 520
            AA      + E+K+   +A T  +++     + + T  SN        T +   N  + 
Sbjct: 398 -AAIIPAKVRPEKKKLEKDAETVNQTSHPTEEEAQVTVSSNADVEDSHETVSPRMNEDRA 431

BLAST of Lsi04G021300 vs. TAIR 10
Match: AT4G32330.3 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 186.8 bits (473), Expect = 4.7e-47
Identity = 167/428 (39.02%), Postives = 225/428 (52.57%), Query Frame = 0

Query: 115 TNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNEKSSGF 174
           T + T+    E S+  +  D     K A    + ++VD  K MK EK Q K ++EK SG 
Sbjct: 38  TVDTTSESQNENSANSSTLDTIEHVKEAAEGTQVEHVDDSKCMKGEKAQRKPRHEKLSGG 97

Query: 175 KHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKVIVSAH 234
           K+ SS  + K+K+GK A+   A  N  G+   +  +  P KS+SFN R+ QV K      
Sbjct: 98  KNNSSVHIKKSKEGKSADAKVAASN--GSVAPNVQTTNPLKSKSFNGREAQVTK------ 157

Query: 235 CLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPRAGDDK 294
                      H       E +DGE  K     K  KK     SE +++SS SP+A D K
Sbjct: 158 --------QGKHDSA--PAESADGEKVKP----KSQKKQAHETSEDDTQSSNSPKADDGK 217

Query: 295 PNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMLR 354
           P +VG LPNYGFSF+C++RAEKR+EFY KLEEK  AKE E N++QAKSKETQEAE++MLR
Sbjct: 218 PRKVGALPNYGFSFKCDQRAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLR 277

Query: 355 KSLNFKATPMPSFYQEPPPPKVELKK------------SSTPAADSSSNDGGDVRSARLS 414
           KSLNFKATPMPSFYQEP PPK ELKK                A+ + S +    R  RLS
Sbjct: 278 KSLNFKATPMPSFYQEPQPPKTELKKIPPTRPKSPKLGRKKTASGADSEETQTPRLGRLS 337

Query: 415 LDENVALNN-NSKRVSPA--RSDKPKRRSLPSLPSEKTTIPGVVTNAGKSSATKVKNVEK 474
           LDE  + +N  +K + P      +P R+SLP LPS+KT +P      GK +  K      
Sbjct: 338 LDERASKDNPTAKGIMPTVDLKKQPVRKSLPRLPSQKTVLPD-----GKPAPAK------ 397

Query: 475 PAAASTTNGKKEEKRASSEAATTKKSACLRSIDEEKTAPSN--------TTNKVANLSQE 520
            AA      + E+K+   +A T  +++     + + T  SN        T +   N  + 
Sbjct: 398 -AAIIPAKVRPEKKKLEKDAETVNQTSHPTEEEAQVTVSSNADVEDSHETVSPRMNEDRA 431

BLAST of Lsi04G021300 vs. TAIR 10
Match: AT4G32330.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 181.8 bits (460), Expect = 1.5e-45
Identity = 166/428 (38.79%), Postives = 225/428 (52.57%), Query Frame = 0

Query: 115 TNELTTRELTEGSSFPAESDISTLSKAAYFEGEEKNVDPPKKMKPEKGQVKSKNEKSSGF 174
           T + T+    E S+  +  D     K A    + ++VD  K MK EK Q K ++EK SG 
Sbjct: 38  TVDTTSESQNENSANSSTLDTIEHVKEAAEGTQVEHVDDSKCMKGEKAQRKPRHEKLSGG 97

Query: 175 KHISSTGVNKNKDGKDAEQIAALLNDSGTGTSHPHSKRPSKSRSFNERQTQVPKVIVSAH 234
           K+ SS  + K+K+GK A+   A  N  G+   +  +  P KS+SFN R+ QV K      
Sbjct: 98  KNNSSVHIKKSKEGKSADAKVAASN--GSVAPNVQTTNPLKSKSFNGREAQVTK------ 157

Query: 235 CLLDSSMLSNTHPPGRQQPEKSDGEGSKENTNLKPLKKGQPSKSEGESESSLSPRAGDDK 294
                      H       E +DGE  K     K  KK     SE +++SS +P+A D K
Sbjct: 158 --------QGKHDSA--PAESADGEKVKP----KSQKKQAHETSEDDTQSS-NPKADDGK 217

Query: 295 PNRVGRLPNYGFSFRCNERAEKRREFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMLR 354
           P +VG LPNYGFSF+C++RAEKR+EFY KLEEK  AKE E N++QAKSKETQEAE++MLR
Sbjct: 218 PRKVGALPNYGFSFKCDQRAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLR 277

Query: 355 KSLNFKATPMPSFYQEPPPPKVELKK------------SSTPAADSSSNDGGDVRSARLS 414
           KSLNFKATPMPSFYQEP PPK ELKK                A+ + S +    R  RLS
Sbjct: 278 KSLNFKATPMPSFYQEPQPPKTELKKIPPTRPKSPKLGRKKTASGADSEETQTPRLGRLS 337

Query: 415 LDENVALNN-NSKRVSPA--RSDKPKRRSLPSLPSEKTTIPGVVTNAGKSSATKVKNVEK 474
           LDE  + +N  +K + P      +P R+SLP LPS+KT +P      GK +  K      
Sbjct: 338 LDERASKDNPTAKGIMPTVDLKKQPVRKSLPRLPSQKTVLPD-----GKPAPAK------ 397

Query: 475 PAAASTTNGKKEEKRASSEAATTKKSACLRSIDEEKTAPSN--------TTNKVANLSQE 520
            AA      + E+K+   +A T  +++     + + T  SN        T +   N  + 
Sbjct: 398 -AAIIPAKVRPEKKKLEKDAETVNQTSHPTEEEAQVTVSSNADVEDSHETVSPRMNEDRA 430

BLAST of Lsi04G021300 vs. TAIR 10
Match: AT2G25480.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 164.1 bits (414), Expect = 3.2e-40
Identity = 145/384 (37.76%), Postives = 200/384 (52.08%), Query Frame = 0

Query: 147 EEKNVDPPKKMKPEKGQVKSKNEKSSGFKHISSTGVNKNKDGKDAEQIAALLNDSGTGTS 206
           + + VD  K+M+ +K Q + K+EK+SG K+I S  V K KDGK       ++  +G+   
Sbjct: 54  QNEQVDDSKQMRGQKVQGRVKHEKTSGGKNIPSVLVKKKKDGK-------VVASNGSVAP 113

Query: 207 HPHSKRPSKSRSFNERQTQVPKVIVSAHCLLDSSMLSNTHPPGRQQPEKSDGEGSKENTN 266
           +    +  KS+S N R+  V K                         +    EG+++   
Sbjct: 114 NVKPVKSPKSKSLNGREAHVTK---------------------HGNHDSLPAEGTRDKPK 173

Query: 267 LKPLKKGQPSKSEGESESSLSPRAGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEE 326
           L+  +K     SE +++    P+  D KP R   LPNYGFSFRC++RAEKRREFYSKLEE
Sbjct: 174 LRETRKQVNDTSEDDTQ---YPKEDDGKPRRASALPNYGFSFRCDQRAEKRREFYSKLEE 233

Query: 327 KIQAKEVEKNTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPPPPKVELKK------ 386
           KI AKE EKNT+QAKSKETQEAE+KMLRKSLNFKATPMP+FYQEP  PK ELKK      
Sbjct: 234 KIHAKEEEKNTVQAKSKETQEAELKMLRKSLNFKATPMPTFYQEPQLPKTELKKIAITRP 293

Query: 387 SSTPAADSSSNDGGDVRSA---------RLSLDENVALNNNSKRVS-PARSDKPK-RRSL 446
            S       +N   D   A         RLSLDE    +N     S P  + KP  R+SL
Sbjct: 294 KSPKLGRKKTNSRADSEEAITIQTPRFGRLSLDEKTPKDNPVVEGSVPGETKKPPVRKSL 353

Query: 447 PSLPSEKTTIPGVVTNAGKSSATKVKNVEKPAAASTTNGKKEEKRASSEAATTKKSACLR 506
           P LPSEKT +     + GK +  K       A  ++T  K E K+   +     +S+ + 
Sbjct: 354 PRLPSEKTNL-----SNGKVAPAK-------AVTASTKAKSERKKPDKDVDDLSQSSPV- 390

Query: 507 SIDEEKTAPSNTTNKVANLSQEEN 514
              ++   P ++  +   ++++ N
Sbjct: 414 ---DDNADPEDSQEQAPRVNEDRN 390

BLAST of Lsi04G021300 vs. TAIR 10
Match: AT2G35880.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 101.3 bits (251), Expect = 2.6e-21
Identity = 91/213 (42.72%), Postives = 115/213 (53.99%), Query Frame = 0

Query: 269 PLKKGQPSKSEGESES-SLSPRAGDDKPNRVGRLPNYGFSFRCNERAEKRREFYSKLEEK 328
           PLK    SK + ++ S + S      + + VG     GFSFR  ERAEKR+EFY KLEEK
Sbjct: 210 PLKAEMASKDDEDTRSTTTSTSTPRGRRSSVGSAS--GFSFRLEERAEKRKEFYMKLEEK 269

Query: 329 IQAKEVEKNTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPPPPKVELKKSSTPAAD 388
           I AKEVEK  LQAKSKE+QE EIK LRKSL FKA PMPSFY+E PPPKVELKK  T    
Sbjct: 270 IHAKEVEKTNLQAKSKESQEEEIKRLRKSLTFKAGPMPSFYKE-PPPKVELKKIPT-TRP 329

Query: 389 SSSNDGGDVRSARLSLDENVALNNNSKRVSPARSDKPKRRSLPSLPSEKTTIPGVVTNAG 448
            S   G    S+  +  E        K  S +   KP  +S P L +++ ++        
Sbjct: 330 KSPKLGRRKSSSDATGGEAAPRVTKPKDSSSSTVKKPITKSQPKLETQEKSV-------- 389

Query: 449 KSSATKVKNVEKPAAASTTNGKKEEKRASSEAA 481
                K K  +K         + EE++A++ AA
Sbjct: 390 -----KAKEKKKKVKKEEAEKRGEEEKATAVAA 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94C486.6e-4639.02Protein WVD2-like 5 OS=Arabidopsis thaliana OX=3702 GN=WDL5 PE=1 SV=1[more]
Q0WSZ84.6e-3937.76Protein WVD2-like 6 OS=Arabidopsis thaliana OX=3702 GN=WDL6 PE=1 SV=1[more]
Q9SJ623.6e-2042.72Protein WVD2-like 4 OS=Arabidopsis thaliana OX=3702 GN=WDL4 PE=1 SV=2[more]
Q67Y691.4e-1635.98Protein WVD2-like 7 OS=Arabidopsis thaliana OX=3702 GN=WDL7 PE=2 SV=1[more]
Q84WL64.6e-1536.32Protein WVD2-like 3 OS=Arabidopsis thaliana OX=3702 GN=WDL3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3E7Z59.5e-18173.70Protein WVD2-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G... [more]
A0A1S3CR671.3e-17773.33protein WVD2-like 5 OS=Cucumis melo OX=3656 GN=LOC103503819 PE=3 SV=1[more]
A0A5A7T5Q56.3e-17773.15Protein WVD2-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G0... [more]
A0A0A0LGM74.1e-17674.26TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G878190 PE=3 S... [more]
A0A6J1C2592.0e-17572.49protein WVD2-like 5 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111006712 PE... [more]
Match NameE-valueIdentityDescription
XP_038898383.11.1e-20782.95protein WVD2-like 5 [Benincasa hispida][more]
TYK31435.12.0e-18073.70protein WVD2-like 5 [Cucumis melo var. makuwa][more]
XP_008466404.12.6e-17773.33PREDICTED: protein WVD2-like 5 [Cucumis melo][more]
KAA0038812.11.3e-17673.15protein WVD2-like 5 [Cucumis melo var. makuwa][more]
XP_031738605.18.5e-17674.26protein WVD2-like 5 [Cucumis sativus] >KGN60099.1 hypothetical protein Csa_00266... [more]
Match NameE-valueIdentityDescription
AT4G32330.14.7e-4739.02TPX2 (targeting protein for Xklp2) protein family [more]
AT4G32330.34.7e-4739.02TPX2 (targeting protein for Xklp2) protein family [more]
AT4G32330.21.5e-4538.79TPX2 (targeting protein for Xklp2) protein family [more]
AT2G25480.13.2e-4037.76TPX2 (targeting protein for Xklp2) protein family [more]
AT2G35880.12.6e-2142.72TPX2 (targeting protein for Xklp2) protein family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 311..348
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 465..479
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 518..542
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 382..396
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 356..553
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 494..517
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 237..251
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 431..452
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..309
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 252..266
NoneNo IPR availablePANTHERPTHR31358:SF29PROTEIN WVD2-LIKE 5-RELATEDcoord: 51..536
IPR027329TPX2, C-terminalPFAMPF06886TPX2coord: 306..383
e-value: 9.5E-26
score: 89.8
IPR044833Protein WVD2-like 4/5/6PANTHERPTHR31358PROTEIN WVD2-LIKE 4coord: 51..536

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G021300.1Lsi04G021300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
molecular_function GO:0008017 microtubule binding