Lsi04G021260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G021260
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionENTH domain-containing protein
Locationchr04: 28400891 .. 28402852 (+)
RNA-Seq ExpressionLsi04G021260
SyntenyLsi04G021260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCCTAGTACGATCCGGAAGGCGATTGGAGCCGTGAAGGACCAGACGAGTATTGGAATTGCGAAGGTTGCGAGTAATATGGCGCCGGACCTTGAGGTAGCGATCGTGAAGGCGACGAGCCATGACGATGATCCGGCCAGTGAGAAGTACATCAGGGAGATTTTGAGCCTCACATCGTATTCTCGTGGATATGTGAGTGCGTGTGTGTCGGCGATTTCGAAGCGTCTGGCCAAGACGAGGGACTGGATTGTGGCGCTCAAGGCACTCATACTTGTGCACAGGTTGTTGAATGAAGGGGACCCGGTGTTTCAGGAGGAGATCTTGTATGCTACTAGAAGGGGCACGAGGCTGTTGAATATGTCCGATTTTAAGGATGAAGCTCATTCGAGCTCGTGGGATCACTCGGCTTTTGTTCGAACTTATGCATTCTACTTGGATCAACGGCTGGAATTGATGTTGTTTGAGAAGAAAGGTGGTAGTGCAAGGGGAAATTCCCGTGGAGATGATAGATTTGATGGAAGAGATGACTTTAGATCTCCGCCCCCAAGGCCCTACGATAATGGTTATGGCGAGTATAGAGGAGAAAGAGAGTATGGGAATTATGGTGGGATGAGGAGGTCGAGATCTTATGGTGATGTGGGGGAATCTGTGGGGAGGGATGGGGAAGGGCAGGGTCGTAACAACAAGGGGCCTGTGACCCCATTGAGGGAAATGACGATCGAGAGAGTTTTTGGGAAGATGGGACATTTGCAGAGACTGTTGGATCGATTCTTGTCATGTCGTCCAACGGGGTTGGCGAAGAATAGTAGGATGATTTTGTATGCTTTGTATCCTCTAGTGAGGGAGAGTTTTCAATTGTATGCAGATATTTGTGAGGTGTTGGCTGTTTTGCTCGACAAATTCTTTGATATGGAGTATTCTGACTGTATGAAGGCATTTGATGCATATGGTAGTTCAGCTAAGCAGATTGATGAGCTAATTGCATTCTACAATTGGTGTAAAGATACAGGAGTTGCTAGGTCCTCTGAGTATCCAGAGGTGCAGAGAATCACCAGTAAGTTACTGGAGACATTGGAGGAGTTTTTGAGGGAAAGAGGGAAGAGGCCGAAGAGTCCCGAGAGGGAGCCGCCTCCACCTGCTCCTGAAGAGGAAGAACCAGTGCCTGATATGAATGAAATAAAAGCTCTTCCTCCACCTGAAAATTACACTCCACCTCCGCCCGAGCCCGAGCCCCAGCCTGCGCCCAAACCTCAACCACAAGTCACGGAAGACTTGGTCAATCTGAGAGATGATGCAGTTAGTGCAGATGATCAAGGTAATAAATTGGCATTGGCTCTGTTTGCTGGTCCAGCAGCTAATGGTGCAAATGGATCCTGGGAAGCTTTCCCTTCCGATGGACAGCCAGAAGTAACCTCTGCCTGGCAGAACCCGGCTGCTGAATCTGGCAAAGCCGACTGGGAGTTGGCTTTGGTTGAGACAGCAAGCAATTTATCAAGGCAGAAAGCAGCACTCGGCGGTGGACTCGACCCATTATTGTTAAATGGTATGTATGATCAAGGAATGGTTAGGCAGCACACTAGCACCTCACAGCTGAGCGGTGGAAGCGCTAGTAGTGTAGCATTGCCCGGTCCTGGAAACAGCAAAACTCCTGTACTGGCTCTTCCAGCTCCAGATGGAACTGTCCAGGCAGTGAATCAGGATCCTTTCGCAGCATCGTTAAGCGTTCCACCTCCTTCTTATGTGCAAATGGTGGAGATGGAGAAGAAACAGCATCTGCTGACGCAGGAACAGCAGTTATGGCAGCAGTATGCAAGAGATGGGATGCAGGGACAGAGCAGTTTGAACAAAATCAGTAACCCCCCAGGTTACTACAACACGGCCGTGGCACCAATGGCTCCGATGCCCTATGGAATGCCTCCGATGAACGGAATGGGCGGGTATTACTACGTTCCTCAATGA

mRNA sequence

ATGGCGCCTAGTACGATCCGGAAGGCGATTGGAGCCGTGAAGGACCAGACGAGTATTGGAATTGCGAAGGTTGCGAGTAATATGGCGCCGGACCTTGAGGTAGCGATCGTGAAGGCGACGAGCCATGACGATGATCCGGCCAGTGAGAAGTACATCAGGGAGATTTTGAGCCTCACATCGTATTCTCGTGGATATGTGAGTGCGTGTGTGTCGGCGATTTCGAAGCGTCTGGCCAAGACGAGGGACTGGATTGTGGCGCTCAAGGCACTCATACTTGTGCACAGGTTGTTGAATGAAGGGGACCCGGTGTTTCAGGAGGAGATCTTGTATGCTACTAGAAGGGGCACGAGGCTGTTGAATATGTCCGATTTTAAGGATGAAGCTCATTCGAGCTCGTGGGATCACTCGGCTTTTGTTCGAACTTATGCATTCTACTTGGATCAACGGCTGGAATTGATGTTGTTTGAGAAGAAAGGTGGTAGTGCAAGGGGAAATTCCCGTGGAGATGATAGATTTGATGGAAGAGATGACTTTAGATCTCCGCCCCCAAGGCCCTACGATAATGGTTATGGCGAGTATAGAGGAGAAAGAGAGTATGGGAATTATGGTGGGATGAGGAGGTCGAGATCTTATGGTGATGTGGGGGAATCTGTGGGGAGGGATGGGGAAGGGCAGGGTCGTAACAACAAGGGGCCTGTGACCCCATTGAGGGAAATGACGATCGAGAGAGTTTTTGGGAAGATGGGACATTTGCAGAGACTGTTGGATCGATTCTTGTCATGTCGTCCAACGGGGTTGGCGAAGAATAGTAGGATGATTTTGTATGCTTTGTATCCTCTAGTGAGGGAGAGTTTTCAATTGTATGCAGATATTTGTGAGGTGTTGGCTGTTTTGCTCGACAAATTCTTTGATATGGAGTATTCTGACTGTATGAAGGCATTTGATGCATATGGTAGTTCAGCTAAGCAGATTGATGAGCTAATTGCATTCTACAATTGGTGTAAAGATACAGGAGTTGCTAGGTCCTCTGAGTATCCAGAGGTGCAGAGAATCACCAGTAAGTTACTGGAGACATTGGAGGAGTTTTTGAGGGAAAGAGGGAAGAGGCCGAAGAGTCCCGAGAGGGAGCCGCCTCCACCTGCTCCTGAAGAGGAAGAACCAGTGCCTGATATGAATGAAATAAAAGCTCTTCCTCCACCTGAAAATTACACTCCACCTCCGCCCGAGCCCGAGCCCCAGCCTGCGCCCAAACCTCAACCACAAGTCACGGAAGACTTGGTCAATCTGAGAGATGATGCAGTTAGTGCAGATGATCAAGGTAATAAATTGGCATTGGCTCTGTTTGCTGGTCCAGCAGCTAATGGTGCAAATGGATCCTGGGAAGCTTTCCCTTCCGATGGACAGCCAGAAGTAACCTCTGCCTGGCAGAACCCGGCTGCTGAATCTGGCAAAGCCGACTGGGAGTTGGCTTTGGTTGAGACAGCAAGCAATTTATCAAGGCAGAAAGCAGCACTCGGCGGTGGACTCGACCCATTATTGTTAAATGGTATGTATGATCAAGGAATGGTTAGGCAGCACACTAGCACCTCACAGCTGAGCGGTGGAAGCGCTAGTAGTGTAGCATTGCCCGGTCCTGGAAACAGCAAAACTCCTGTACTGGCTCTTCCAGCTCCAGATGGAACTGTCCAGGCAGTGAATCAGGATCCTTTCGCAGCATCGTTAAGCGTTCCACCTCCTTCTTATGTGCAAATGGTGGAGATGGAGAAGAAACAGCATCTGCTGACGCAGGAACAGCAGTTATGGCAGCAGTATGCAAGAGATGGGATGCAGGGACAGAGCAGTTTGAACAAAATCAGTAACCCCCCAGGTTACTACAACACGGCCGTGGCACCAATGGCTCCGATGCCCTATGGAATGCCTCCGATGAACGGAATGGGCGGGTATTACTACGTTCCTCAATGA

Coding sequence (CDS)

ATGGCGCCTAGTACGATCCGGAAGGCGATTGGAGCCGTGAAGGACCAGACGAGTATTGGAATTGCGAAGGTTGCGAGTAATATGGCGCCGGACCTTGAGGTAGCGATCGTGAAGGCGACGAGCCATGACGATGATCCGGCCAGTGAGAAGTACATCAGGGAGATTTTGAGCCTCACATCGTATTCTCGTGGATATGTGAGTGCGTGTGTGTCGGCGATTTCGAAGCGTCTGGCCAAGACGAGGGACTGGATTGTGGCGCTCAAGGCACTCATACTTGTGCACAGGTTGTTGAATGAAGGGGACCCGGTGTTTCAGGAGGAGATCTTGTATGCTACTAGAAGGGGCACGAGGCTGTTGAATATGTCCGATTTTAAGGATGAAGCTCATTCGAGCTCGTGGGATCACTCGGCTTTTGTTCGAACTTATGCATTCTACTTGGATCAACGGCTGGAATTGATGTTGTTTGAGAAGAAAGGTGGTAGTGCAAGGGGAAATTCCCGTGGAGATGATAGATTTGATGGAAGAGATGACTTTAGATCTCCGCCCCCAAGGCCCTACGATAATGGTTATGGCGAGTATAGAGGAGAAAGAGAGTATGGGAATTATGGTGGGATGAGGAGGTCGAGATCTTATGGTGATGTGGGGGAATCTGTGGGGAGGGATGGGGAAGGGCAGGGTCGTAACAACAAGGGGCCTGTGACCCCATTGAGGGAAATGACGATCGAGAGAGTTTTTGGGAAGATGGGACATTTGCAGAGACTGTTGGATCGATTCTTGTCATGTCGTCCAACGGGGTTGGCGAAGAATAGTAGGATGATTTTGTATGCTTTGTATCCTCTAGTGAGGGAGAGTTTTCAATTGTATGCAGATATTTGTGAGGTGTTGGCTGTTTTGCTCGACAAATTCTTTGATATGGAGTATTCTGACTGTATGAAGGCATTTGATGCATATGGTAGTTCAGCTAAGCAGATTGATGAGCTAATTGCATTCTACAATTGGTGTAAAGATACAGGAGTTGCTAGGTCCTCTGAGTATCCAGAGGTGCAGAGAATCACCAGTAAGTTACTGGAGACATTGGAGGAGTTTTTGAGGGAAAGAGGGAAGAGGCCGAAGAGTCCCGAGAGGGAGCCGCCTCCACCTGCTCCTGAAGAGGAAGAACCAGTGCCTGATATGAATGAAATAAAAGCTCTTCCTCCACCTGAAAATTACACTCCACCTCCGCCCGAGCCCGAGCCCCAGCCTGCGCCCAAACCTCAACCACAAGTCACGGAAGACTTGGTCAATCTGAGAGATGATGCAGTTAGTGCAGATGATCAAGGTAATAAATTGGCATTGGCTCTGTTTGCTGGTCCAGCAGCTAATGGTGCAAATGGATCCTGGGAAGCTTTCCCTTCCGATGGACAGCCAGAAGTAACCTCTGCCTGGCAGAACCCGGCTGCTGAATCTGGCAAAGCCGACTGGGAGTTGGCTTTGGTTGAGACAGCAAGCAATTTATCAAGGCAGAAAGCAGCACTCGGCGGTGGACTCGACCCATTATTGTTAAATGGTATGTATGATCAAGGAATGGTTAGGCAGCACACTAGCACCTCACAGCTGAGCGGTGGAAGCGCTAGTAGTGTAGCATTGCCCGGTCCTGGAAACAGCAAAACTCCTGTACTGGCTCTTCCAGCTCCAGATGGAACTGTCCAGGCAGTGAATCAGGATCCTTTCGCAGCATCGTTAAGCGTTCCACCTCCTTCTTATGTGCAAATGGTGGAGATGGAGAAGAAACAGCATCTGCTGACGCAGGAACAGCAGTTATGGCAGCAGTATGCAAGAGATGGGATGCAGGGACAGAGCAGTTTGAACAAAATCAGTAACCCCCCAGGTTACTACAACACGGCCGTGGCACCAATGGCTCCGATGCCCTATGGAATGCCTCCGATGAACGGAATGGGCGGGTATTACTACGTTCCTCAATGA

Protein sequence

MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRSPPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLEEFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQPQVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ
Homology
BLAST of Lsi04G021260 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 931.0 bits (2405), Expect = 7.3e-270
Identity = 516/671 (76.90%), Postives = 568/671 (84.65%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPS IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
            SRGY+ ACV+++S+RL+KTRDW+VALKAL+LVHRLLNEGDP+FQEEILY+TRRGTR+LN
Sbjct: 61  LSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKK-------GGSARGNSRGDDRFD 180
           MSDF+DEAHSSSWDHSAFVRTYA YLDQRLEL LFE+K       GG++  +S  DDR+ 
Sbjct: 121 MSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYG 180

Query: 181 -GRDDFRSPPPRPYD--NGYG---EYRGEREYGNYGGM-RRSRSYGDVGESVGRDGEGQG 240
            GRDDFRSPPPR YD  NG G   ++RG+     YGG+ +RSRSYGD+ E +G  G G G
Sbjct: 181 RGRDDFRSPPPRSYDYENGGGGGSDFRGDN--NGYGGVPKRSRSYGDMTE-MGGGGGGGG 240

Query: 241 RNNKGPVTPLREMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQ 300
           R+ K  VTPLREMT ER+FGKMGHLQRLLDRFLS RPTGLAKNSRMIL ALYP+VRESF+
Sbjct: 241 RDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFK 300

Query: 301 LYADICEVLAVLLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYP 360
           LYADICEVLAVLLDKFFDMEYSDC+KAFDAY S+AKQIDELIAFYNWCK+TGVARSSEYP
Sbjct: 301 LYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYP 360

Query: 361 EVQRITSKLLETLEEFLRERGKRPKSPER---EPPPPAPEEEEPVPDMNEIKALPPPENY 420
           EVQRITSKLLETLEEF+R+R KR KSPER   E PPP  EEEEP PDMNEIKALPPPENY
Sbjct: 361 EVQRITSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENY 420

Query: 421 TPPPPEPEPQPAPKPQPQVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAF 480
           TPPPP PEP+P P+ +PQ TEDLVNLR+D V+ADDQGNK ALALFAGP  N  NG WEAF
Sbjct: 421 TPPPP-PEPEPQPE-KPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGN--NGKWEAF 480

Query: 481 PSDGQPEVTSAWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMV 540
            S+G   VTSAWQNPAAE GKADWELALVET SNL +Q AALGGG D LLLNGMYDQGMV
Sbjct: 481 SSNG---VTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMV 540

Query: 541 RQHTSTSQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQ 600
           RQH STSQL+GGSASSVALP PG +   VLALPAPDGTV+ VNQDPFAASL++PPPSYVQ
Sbjct: 541 RQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVEKVNQDPFAASLTIPPPSYVQ 600

Query: 601 MVEMEKKQHLLTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMN 651
           M EMEKKQ+LL+QEQQLWQQY RDGM+GQ+SL K+       NT   P     YGMPP+N
Sbjct: 601 MAEMEKKQYLLSQEQQLWQQYQRDGMRGQASLAKM-------NTGPVP----AYGMPPVN 649

BLAST of Lsi04G021260 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 904.0 bits (2335), Expect = 9.5e-262
Identity = 500/661 (75.64%), Postives = 554/661 (83.81%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTSYSRG 64
           ++RKAIG VKDQTSIGIAKVASNMAPDLEVAIVKATSHDDD +S+KYIREILSLTS SRG
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDF 124
           YV ACV+++S+RL KTRDWIVALKAL+LVHRLLNEGDP+FQEEILYATRRGTR+LNMSDF
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 KDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGN-----SRGDDRFD-GRDDF 184
           +DEAHSSSWDHSAFVRTYA YLDQRLEL LFE++G +  G+     S GDD ++  RDDF
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 RSPPPRPYDNGYGEYRGEREYGNYGGM-RRSRSYGDVGESVGRDGEGQGRNNKGPVTPLR 244
           RSPPPR YD          E GN  GM +RSRS+GDV E +G       R  K  VTPLR
Sbjct: 184 RSPPPRTYD---------YETGNGFGMPKRSRSFGDVNE-IG------AREEKKSVTPLR 243

Query: 245 EMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAV 304
           EMT ER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMIL A+YP+V+ESF+LYADICEVLAV
Sbjct: 244 EMTPERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAV 303

Query: 305 LLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLE 364
           LLDKFFDMEY+DC+KAFDAY S+AKQIDELIAFY+WCKDTGVARSSEYPEVQRITSKLLE
Sbjct: 304 LLDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLE 363

Query: 365 TLEEFLRERGKRPKSPERE----PPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQ 424
           TLEEF+R+R KR KSPER+    PP PAP  EEPV DMNEIKALPPPEN+TPPPP P P+
Sbjct: 364 TLEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPV-DMNEIKALPPPENHTPPPP-PAPE 423

Query: 425 PAPKPQPQVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTS 484
           P P+ QPQVT+DLVNLR+D VS DDQGNK ALALFAGP AN  NG WEAF SD    VTS
Sbjct: 424 PKPQ-QPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTS 483

Query: 485 AWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLS 544
           AWQNPAAE GKADWELALVETASNL  QKAA+GGGLDPLLLNGMYDQG VRQH STS+L+
Sbjct: 484 AWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELT 543

Query: 545 GGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHL 604
           GGS+SSVALP PG   + +LALPAPDGTVQ VNQDPFAASL++PPPSYVQM EM+KKQ+L
Sbjct: 544 GGSSSSVALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYL 603

Query: 605 LTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMG----GYY 651
           LTQEQQLWQQY ++GM+GQ+SL K+       NTA      MPYGMPP+NGMG    GYY
Sbjct: 604 LTQEQQLWQQYQQEGMRGQASLAKM-------NTA---QTAMPYGMPPVNGMGPSPMGYY 631

BLAST of Lsi04G021260 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 4.3e-129
Identity = 296/659 (44.92%), Postives = 399/659 (60.55%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMA--PDLEVAIVKATSHDDDPASEKYIREILSL 60
           M  S  ++AIGAVKDQTS+G+AKV    A   +L+VAIVKAT H++ PA EKYIREILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR Y++ACVS +S+RL KT+ W VALK LIL+ RLL EGD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDF 180
           LNMSDF+D + S+SWD+SAFVRTYA YLD+RL+  +       AR   RG     G  D 
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRM------QARHGKRGVYCVGGEAD- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLRE 240
                            E E        + ++  D+  ++    +           P+ E
Sbjct: 181 -----------------EEE--------QDQAAADLSTAIVVRSQ-----------PIAE 240

Query: 241 MTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 300
           M  E++F ++ HLQ+LLDRFL+CRPTG A+N+R+++ ALYP+V+ESFQ+Y D+ E++ +L
Sbjct: 241 MKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKESFQIYYDVTEIMGIL 300

Query: 301 LDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLET 360
           +++F +++  D +K +D +   +KQ +EL  FY+WCK+ G+ARSSEYPE+++IT K L+ 
Sbjct: 301 IERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSSEYPEIEKITQKKLDL 360

Query: 361 LEEFLRER--------GKRPKSPEREPPPPAPEEE--EPVPDMNEIKALPPPENYTPPPP 420
           ++EF+R++         K  KS   E    A  EE  E   DMN IKALP P    PP  
Sbjct: 361 MDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDMNAIKALPEP----PPKE 420

Query: 421 EPEPQPAPKPQPQV-----TEDLVNLRD----DAVSADDQGNKLALALFAGPAANGANG- 480
           E + +P  + + +V      E++ +L D    +   A   G+ LALALF GP A+G+   
Sbjct: 421 EDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDGPYASGSGSE 480

Query: 481 ---SWEAFPSDGQPEVTSAWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLN 540
               WEAF  D                  ADWE ALV+TA+NLS QK+ LGGG D LLLN
Sbjct: 481 SGPGWEAFKDD-----------------SADWETALVQTATNLSGQKSELGGGFDMLLLN 540

Query: 541 GMYDQGMVRQ--HTSTSQLSGGSASSVALPGPGNSKTPVLALPAPD------GTVQA-VN 600
           GMY  G V     TST+  + GSASS+A    G     +LALPAP       G + + V 
Sbjct: 541 GMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTANGNAGNINSPVP 595

Query: 601 QDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQLWQQYARDGMQGQSSLNKISNPPGYY 626
            DPFAASL V PP+YVQM +MEKKQ +L +EQ +W QY+RDG QG  +L +  N P  Y
Sbjct: 601 MDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHMNLRQNQNQPYSY 595

BLAST of Lsi04G021260 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 451.4 bits (1160), Expect = 1.7e-125
Identity = 300/683 (43.92%), Postives = 398/683 (58.27%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVA--SNMAPDLEVAIVKATSHDDDPASEKYIREILSL 60
           M  S +++AIGAVKDQTS+G+AKV   S+   +LE+A+VKAT HDD PA +KYIREIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR YVSACV+ +S+RL KT++W VALK LIL+ RLL +GD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDF 180
           LNMSDF+D + S SWD+SAFVRTYA YLD+RL                  D R  GR   
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERL------------------DYRMQGR--- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVT---P 240
                          RG+++ G  GG       GD GE     G      +K  V    P
Sbjct: 181 ---------------RGKKKSGGGGG-----GDGDSGEEDDHRGTSNDIRSKAIVVKSKP 240

Query: 241 LREMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVL 300
           + EM  E++F ++ HLQ+LLDRFL+CRPTG AKN+R+++ A+YP+V+ESFQLY +I E++
Sbjct: 241 VAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAMYPIVKESFQLYYNITEIM 300

Query: 301 AVLLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKL 360
            VL+++F +++  D +K ++ +   +KQ DEL  FY WCK+  VARSSEYPE+++IT K 
Sbjct: 301 GVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARSSEYPELEKITQKK 360

Query: 361 LETLEEFLRER----GKRPKSPEREPPPPAPEE------EEPVPDMNEIKALPPPENYTP 420
           L+ ++EF+R++     +  KS  +       EE      +E   D+N IKALP PE    
Sbjct: 361 LDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQEDLNSIKALPAPEQKEE 420

Query: 421 PPPEPEPQPAPKPQPQVTE-----DLVNLRDDA-VSADDQGNKLALALFAGPAA--NGAN 480
              E + +     +  V+      DL++L D+A V+A   G+ LALALF G     + + 
Sbjct: 421 EEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALALFDGVVGTESASG 480

Query: 481 GSWEAFPSDGQPEVTSAWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGM 540
             WEAF                     ADWE  LV +A+ LS QK+ LGGG D LLL+GM
Sbjct: 481 PGWEAF-----------------NDNSADWETDLVRSATRLSGQKSELGGGFDTLLLDGM 540

Query: 541 YDQGMVRQ--HTSTSQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQA-----VNQDPF 600
           Y  G V     TST+  S GSASSVA    G+    +LALPAP  T        V  DPF
Sbjct: 541 YQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTANGNRNSPVMVDPF 600

Query: 601 AASLSVPPPSYVQMVEMEKKQHLLTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVA 654
           AASL V PP+YVQM +MEKKQ LL +EQ +W QY R G QG  +  + +    YY     
Sbjct: 601 AASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQGHMNFGQ-NQQQQYYQ---- 610

BLAST of Lsi04G021260 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 8.0e-67
Identity = 154/398 (38.69%), Postives = 231/398 (58.04%), Query Frame = 0

Query: 3   PSTIRKAIGAVKDQTSIGIAKVASNMA-----PDLEVAIVKATSHDDD-PASEKYIREIL 62
           PS ++KAIGAVKDQTSI +AKVA+          LEVAI+KATSHD++ P  ++ + EIL
Sbjct: 2   PSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEIL 61

Query: 63  SLTSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGT 122
            + S  + + ++C +AI +R+ +TR+WIVALK+L+LV R+  +GDP F  E+L+A +RG 
Sbjct: 62  GIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGA 121

Query: 123 RLLNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRD 182
           ++LN+S F+D+++S  WD +AFVRT+A YLD+RL+  L  K                   
Sbjct: 122 KILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKL------------------ 181

Query: 183 DFRSPPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPL 242
                              +R Y N     R ++      S  R         K     +
Sbjct: 182 -------------------QRRYTN-----REQTGRISTNSTTRSRFNPKAGIKSHEPAV 241

Query: 243 REMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLA 302
           R+M    +  K+ + Q+LLDR ++ RPTG AK +R++  +LY +++ESF LY DI + LA
Sbjct: 242 RDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSLYAVMQESFDLYRDISDGLA 301

Query: 303 VLLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLL 362
           +LLD FF ++Y  C+ AF A   ++KQ +EL AFY+  K  G+ R+SEYP +Q+I+ +LL
Sbjct: 302 LLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSIGIGRTSEYPSIQKISLELL 357

Query: 363 ETLEEFLRERGKRP------KSPEREPPPPAPEEEEPV 389
           ETL+EFL+++   P       SP    PPP   ++  V
Sbjct: 362 ETLQEFLKDQSSFPASSGLYPSPNSFLPPPPSSKDSAV 357

BLAST of Lsi04G021260 vs. ExPASy TrEMBL
Match: A0A1S3CRD1 (putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC103503830 PE=4 SV=1)

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 640/653 (98.01%), Postives = 646/653 (98.93%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGES GRDG+GQGRNNKGPVTPLREMT
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRNNKGPVTPLREMT 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGKRPKSPEREPPPPAPEEEEP PDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVT+DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNKISNPPGYYN  VAPMAPMPYGMPPMNGMGGYYYVPQ
Sbjct: 601 WQQYARDGMQGQSSLNKISNPPGYYNMGVAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of Lsi04G021260 vs. ExPASy TrEMBL
Match: A0A0A0LJA4 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G877670 PE=4 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 635/653 (97.24%), Postives = 643/653 (98.47%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRD+FRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDEFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGES GRDG+GQGR NKGPVTPLREMT
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRINKGPVTPLREMT 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGS+AKQIDELIAFYNWCK+TGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKETGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGKRPKSPEREPPPPAPEEEEP PDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVT+DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSL KISNPPGYYN   APMAPMPYGMPPMNGMGGYYYVPQ
Sbjct: 601 WQQYARDGMQGQSSLTKISNPPGYYNMGAAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of Lsi04G021260 vs. ExPASy TrEMBL
Match: A0A6J1IL73 (putative clathrin assembly protein At2g25430 OS=Cucurbita maxima OX=3661 GN=LOC111476611 PE=4 SV=1)

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 619/653 (94.79%), Postives = 636/653 (97.40%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGSARGNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPY+NGYGEYRGERE GNYGGMRRSRS+GDVGES+ R+G+GQGR NKGPVTPLREM 
Sbjct: 181 PPPRPYENGYGEYRGERENGNYGGMRRSRSFGDVGESMEREGQGQGR-NKGPVTPLREMP 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IER+FGKMGHLQRLLDRFLSCRPTGLAK+SRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKSSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGK PKSPEREPPPPAP+EEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVTEDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLS GSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSSGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQA+NQDPFAASLS+PPPSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQALNQDPFAASLSIPPPSYVQMVEMEKKQHLLVQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNK+SNPPGYYNTA  PMAPMPYGMPPMNG  GYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNG-SGYYYIPQ 651

BLAST of Lsi04G021260 vs. ExPASy TrEMBL
Match: A0A6J1F990 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111443479 PE=4 SV=1)

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 619/653 (94.79%), Postives = 635/653 (97.24%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGSARGNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDNGYGEYRGEREYGNYGGMRRSRS+GDVGES+ R+G+G+   NKGPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSFGDVGESMEREGQGR---NKGPVTPLREMP 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGK PKSPEREPPPPAP+EEEPVPDMNEIKALPPPENYTPPPPEPEPQPAP PQP
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPIPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVTEDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHT+T+QLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTNTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQH+L QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHMLVQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNK+SNPPGYYNTA  PMAPMPYGMPPMNG  GYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNG-SGYYYIPQ 649

BLAST of Lsi04G021260 vs. ExPASy TrEMBL
Match: A0A6J1FFH4 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111443478 PE=4 SV=1)

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 616/653 (94.33%), Postives = 633/653 (96.94%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACV A+SKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVVAVSKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKG SA+GNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGSSAKGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDNGYGEYRGEREYGNYGGMRRSRS+GDVGES+ R+G+G+   NKGPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSFGDVGESMEREGQGR---NKGPVTPLREMP 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGK PKSPEREPPPPAP+EEEPVPDMNEIKALPPPENYTPPPPEPEPQPAP PQP
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPIPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVTEDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHT+T+QLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTNTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQH+L QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHMLVQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNK+SNPPGYYN A  PMAPMPYGMPPMNG  GYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNMAAGPMAPMPYGMPPMNG-SGYYYIPQ 649

BLAST of Lsi04G021260 vs. NCBI nr
Match: XP_008466416.1 (PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 640/653 (98.01%), Postives = 646/653 (98.93%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGES GRDG+GQGRNNKGPVTPLREMT
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRNNKGPVTPLREMT 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGKRPKSPEREPPPPAPEEEEP PDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVT+DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNKISNPPGYYN  VAPMAPMPYGMPPMNGMGGYYYVPQ
Sbjct: 601 WQQYARDGMQGQSSLNKISNPPGYYNMGVAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of Lsi04G021260 vs. NCBI nr
Match: XP_004136352.1 (putative clathrin assembly protein At2g25430 [Cucumis sativus] >KGN60096.1 hypothetical protein Csa_001389 [Cucumis sativus])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 635/653 (97.24%), Postives = 643/653 (98.47%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRD+FRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDEFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGES GRDG+GQGR NKGPVTPLREMT
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRINKGPVTPLREMT 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGS+AKQIDELIAFYNWCK+TGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKETGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGKRPKSPEREPPPPAPEEEEP PDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVT+DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSL KISNPPGYYN   APMAPMPYGMPPMNGMGGYYYVPQ
Sbjct: 601 WQQYARDGMQGQSSLTKISNPPGYYNMGAAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of Lsi04G021260 vs. NCBI nr
Match: XP_038898099.1 (putative clathrin assembly protein At2g25430 [Benincasa hispida])

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 634/653 (97.09%), Postives = 640/653 (98.01%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGS RGNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDN YGEYRGEREYGNYGGMRRSRSYGDVGESVGRDG+GQG NNKGPVTPLREM 
Sbjct: 181 PPPRPYDNSYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGQGQGHNNKGPVTPLREMA 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVTEDLVNLRDD VSADDQGNKLALALFAGP ANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTEDLVNLRDDGVSADDQGNKLALALFAGPVANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTP+LALPAPDGTVQ VNQDPFA SLSVP PSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPILALPAPDGTVQTVNQDPFATSLSVPAPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNKISNPPGYYNT +APMAPMPYGMPPMNGMGGYYYVPQ
Sbjct: 601 WQQYARDGMQGQSSLNKISNPPGYYNTGMAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of Lsi04G021260 vs. NCBI nr
Match: KAG6591568.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG6591605.1 putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 620/653 (94.95%), Postives = 635/653 (97.24%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGSARGNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPYDNGYGEYRGEREYGNYGGMRRSRS+GDVGES+ R+G+G+   NKGPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSFGDVGESMEREGQGR---NKGPVTPLREMP 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGK PKSPEREPPPPAP+EEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVTEDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWEL LVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLSGGSASSV
Sbjct: 481 EPGKADWELTLVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLS+PPPSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSIPPPSYVQMVEMEKKQHLLVQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNK+SNPPGYYNTA  PMAPMPYGMPPMNG  GYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNG-SGYYYIPQ 649

BLAST of Lsi04G021260 vs. NCBI nr
Match: XP_022976123.1 (putative clathrin assembly protein At2g25430 [Cucurbita maxima])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 619/653 (94.79%), Postives = 636/653 (97.40%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREILSLTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGSARGNSRGDDRFDGRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLREMT 240
           PPPRPY+NGYGEYRGERE GNYGGMRRSRS+GDVGES+ R+G+GQGR NKGPVTPLREM 
Sbjct: 181 PPPRPYENGYGEYRGERENGNYGGMRRSRSFGDVGESMEREGQGQGR-NKGPVTPLREMP 240

Query: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
           IER+FGKMGHLQRLLDRFLSCRPTGLAK+SRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKSSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGS+AKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420
           EFLRERGK PKSPEREPPPPAP+EEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQNPAA 480
           QVTEDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQ PAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 ESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLSGGSASSV 540
           E GKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTST+QLS GSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSSGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQL 600
           ALPGPGNSKTPVLALPAPDGTVQA+NQDPFAASLS+PPPSYVQMVEMEKKQHLL QEQQL
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQALNQDPFAASLSIPPPSYVQMVEMEKKQHLLVQEQQL 600

Query: 601 WQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMGGYYYVPQ 654
           WQQYARDGMQGQSSLNK+SNPPGYYNTA  PMAPMPYGMPPMNG  GYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNG-SGYYYIPQ 651

BLAST of Lsi04G021260 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 931.0 bits (2405), Expect = 5.2e-271
Identity = 516/671 (76.90%), Postives = 568/671 (84.65%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60
           MAPS IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
            SRGY+ ACV+++S+RL+KTRDW+VALKAL+LVHRLLNEGDP+FQEEILY+TRRGTR+LN
Sbjct: 61  LSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKK-------GGSARGNSRGDDRFD 180
           MSDF+DEAHSSSWDHSAFVRTYA YLDQRLEL LFE+K       GG++  +S  DDR+ 
Sbjct: 121 MSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYG 180

Query: 181 -GRDDFRSPPPRPYD--NGYG---EYRGEREYGNYGGM-RRSRSYGDVGESVGRDGEGQG 240
            GRDDFRSPPPR YD  NG G   ++RG+     YGG+ +RSRSYGD+ E +G  G G G
Sbjct: 181 RGRDDFRSPPPRSYDYENGGGGGSDFRGDN--NGYGGVPKRSRSYGDMTE-MGGGGGGGG 240

Query: 241 RNNKGPVTPLREMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQ 300
           R+ K  VTPLREMT ER+FGKMGHLQRLLDRFLS RPTGLAKNSRMIL ALYP+VRESF+
Sbjct: 241 RDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFK 300

Query: 301 LYADICEVLAVLLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYP 360
           LYADICEVLAVLLDKFFDMEYSDC+KAFDAY S+AKQIDELIAFYNWCK+TGVARSSEYP
Sbjct: 301 LYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYP 360

Query: 361 EVQRITSKLLETLEEFLRERGKRPKSPER---EPPPPAPEEEEPVPDMNEIKALPPPENY 420
           EVQRITSKLLETLEEF+R+R KR KSPER   E PPP  EEEEP PDMNEIKALPPPENY
Sbjct: 361 EVQRITSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENY 420

Query: 421 TPPPPEPEPQPAPKPQPQVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAF 480
           TPPPP PEP+P P+ +PQ TEDLVNLR+D V+ADDQGNK ALALFAGP  N  NG WEAF
Sbjct: 421 TPPPP-PEPEPQPE-KPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGN--NGKWEAF 480

Query: 481 PSDGQPEVTSAWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMV 540
            S+G   VTSAWQNPAAE GKADWELALVET SNL +Q AALGGG D LLLNGMYDQGMV
Sbjct: 481 SSNG---VTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMV 540

Query: 541 RQHTSTSQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQ 600
           RQH STSQL+GGSASSVALP PG +   VLALPAPDGTV+ VNQDPFAASL++PPPSYVQ
Sbjct: 541 RQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVEKVNQDPFAASLTIPPPSYVQ 600

Query: 601 MVEMEKKQHLLTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMN 651
           M EMEKKQ+LL+QEQQLWQQY RDGM+GQ+SL K+       NT   P     YGMPP+N
Sbjct: 601 MAEMEKKQYLLSQEQQLWQQYQRDGMRGQASLAKM-------NTGPVP----AYGMPPVN 649

BLAST of Lsi04G021260 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 904.0 bits (2335), Expect = 6.8e-263
Identity = 500/661 (75.64%), Postives = 554/661 (83.81%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTSYSRG 64
           ++RKAIG VKDQTSIGIAKVASNMAPDLEVAIVKATSHDDD +S+KYIREILSLTS SRG
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDF 124
           YV ACV+++S+RL KTRDWIVALKAL+LVHRLLNEGDP+FQEEILYATRRGTR+LNMSDF
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 KDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGN-----SRGDDRFD-GRDDF 184
           +DEAHSSSWDHSAFVRTYA YLDQRLEL LFE++G +  G+     S GDD ++  RDDF
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 RSPPPRPYDNGYGEYRGEREYGNYGGM-RRSRSYGDVGESVGRDGEGQGRNNKGPVTPLR 244
           RSPPPR YD          E GN  GM +RSRS+GDV E +G       R  K  VTPLR
Sbjct: 184 RSPPPRTYD---------YETGNGFGMPKRSRSFGDVNE-IG------AREEKKSVTPLR 243

Query: 245 EMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAV 304
           EMT ER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMIL A+YP+V+ESF+LYADICEVLAV
Sbjct: 244 EMTPERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAV 303

Query: 305 LLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLE 364
           LLDKFFDMEY+DC+KAFDAY S+AKQIDELIAFY+WCKDTGVARSSEYPEVQRITSKLLE
Sbjct: 304 LLDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLE 363

Query: 365 TLEEFLRERGKRPKSPERE----PPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQ 424
           TLEEF+R+R KR KSPER+    PP PAP  EEPV DMNEIKALPPPEN+TPPPP P P+
Sbjct: 364 TLEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPV-DMNEIKALPPPENHTPPPP-PAPE 423

Query: 425 PAPKPQPQVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTS 484
           P P+ QPQVT+DLVNLR+D VS DDQGNK ALALFAGP AN  NG WEAF SD    VTS
Sbjct: 424 PKPQ-QPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTS 483

Query: 485 AWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLS 544
           AWQNPAAE GKADWELALVETASNL  QKAA+GGGLDPLLLNGMYDQG VRQH STS+L+
Sbjct: 484 AWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELT 543

Query: 545 GGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHL 604
           GGS+SSVALP PG   + +LALPAPDGTVQ VNQDPFAASL++PPPSYVQM EM+KKQ+L
Sbjct: 544 GGSSSSVALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYL 603

Query: 605 LTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMG----GYY 651
           LTQEQQLWQQY ++GM+GQ+SL K+       NTA      MPYGMPP+NGMG    GYY
Sbjct: 604 LTQEQQLWQQYQQEGMRGQASLAKM-------NTA---QTAMPYGMPPVNGMGPSPMGYY 631

BLAST of Lsi04G021260 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 904.0 bits (2335), Expect = 6.8e-263
Identity = 500/661 (75.64%), Postives = 554/661 (83.81%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTSYSRG 64
           ++RKAIG VKDQTSIGIAKVASNMAPDLEVAIVKATSHDDD +S+KYIREILSLTS SRG
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDF 124
           YV ACV+++S+RL KTRDWIVALKAL+LVHRLLNEGDP+FQEEILYATRRGTR+LNMSDF
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 KDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGN-----SRGDDRFD-GRDDF 184
           +DEAHSSSWDHSAFVRTYA YLDQRLEL LFE++G +  G+     S GDD ++  RDDF
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 RSPPPRPYDNGYGEYRGEREYGNYGGM-RRSRSYGDVGESVGRDGEGQGRNNKGPVTPLR 244
           RSPPPR YD          E GN  GM +RSRS+GDV E +G       R  K  VTPLR
Sbjct: 184 RSPPPRTYD---------YETGNGFGMPKRSRSFGDVNE-IG------AREEKKSVTPLR 243

Query: 245 EMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAV 304
           EMT ER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMIL A+YP+V+ESF+LYADICEVLAV
Sbjct: 244 EMTPERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAV 303

Query: 305 LLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLE 364
           LLDKFFDMEY+DC+KAFDAY S+AKQIDELIAFY+WCKDTGVARSSEYPEVQRITSKLLE
Sbjct: 304 LLDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLE 363

Query: 365 TLEEFLRERGKRPKSPERE----PPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQ 424
           TLEEF+R+R KR KSPER+    PP PAP  EEPV DMNEIKALPPPEN+TPPPP P P+
Sbjct: 364 TLEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPV-DMNEIKALPPPENHTPPPP-PAPE 423

Query: 425 PAPKPQPQVTEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTS 484
           P P+ QPQVT+DLVNLR+D VS DDQGNK ALALFAGP AN  NG WEAF SD    VTS
Sbjct: 424 PKPQ-QPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTS 483

Query: 485 AWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTSQLS 544
           AWQNPAAE GKADWELALVETASNL  QKAA+GGGLDPLLLNGMYDQG VRQH STS+L+
Sbjct: 484 AWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELT 543

Query: 545 GGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHL 604
           GGS+SSVALP PG   + +LALPAPDGTVQ VNQDPFAASL++PPPSYVQM EM+KKQ+L
Sbjct: 544 GGSSSSVALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYL 603

Query: 605 LTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVAPMAPMPYGMPPMNGMG----GYY 651
           LTQEQQLWQQY ++GM+GQ+SL K+       NTA      MPYGMPP+NGMG    GYY
Sbjct: 604 LTQEQQLWQQYQQEGMRGQASLAKM-------NTA---QTAMPYGMPPVNGMGPSPMGYY 631

BLAST of Lsi04G021260 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 463.4 bits (1191), Expect = 3.0e-130
Identity = 296/659 (44.92%), Postives = 399/659 (60.55%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMA--PDLEVAIVKATSHDDDPASEKYIREILSL 60
           M  S  ++AIGAVKDQTS+G+AKV    A   +L+VAIVKAT H++ PA EKYIREILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR Y++ACVS +S+RL KT+ W VALK LIL+ RLL EGD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDF 180
           LNMSDF+D + S+SWD+SAFVRTYA YLD+RL+  +       AR   RG     G  D 
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRM------QARHGKRGVYCVGGEAD- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVTPLRE 240
                            E E        + ++  D+  ++    +           P+ E
Sbjct: 181 -----------------EEE--------QDQAAADLSTAIVVRSQ-----------PIAE 240

Query: 241 MTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 300
           M  E++F ++ HLQ+LLDRFL+CRPTG A+N+R+++ ALYP+V+ESFQ+Y D+ E++ +L
Sbjct: 241 MKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKESFQIYYDVTEIMGIL 300

Query: 301 LDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLET 360
           +++F +++  D +K +D +   +KQ +EL  FY+WCK+ G+ARSSEYPE+++IT K L+ 
Sbjct: 301 IERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSSEYPEIEKITQKKLDL 360

Query: 361 LEEFLRER--------GKRPKSPEREPPPPAPEEE--EPVPDMNEIKALPPPENYTPPPP 420
           ++EF+R++         K  KS   E    A  EE  E   DMN IKALP P    PP  
Sbjct: 361 MDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDMNAIKALPEP----PPKE 420

Query: 421 EPEPQPAPKPQPQV-----TEDLVNLRD----DAVSADDQGNKLALALFAGPAANGANG- 480
           E + +P  + + +V      E++ +L D    +   A   G+ LALALF GP A+G+   
Sbjct: 421 EDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDGPYASGSGSE 480

Query: 481 ---SWEAFPSDGQPEVTSAWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLN 540
               WEAF  D                  ADWE ALV+TA+NLS QK+ LGGG D LLLN
Sbjct: 481 SGPGWEAFKDD-----------------SADWETALVQTATNLSGQKSELGGGFDMLLLN 540

Query: 541 GMYDQGMVRQ--HTSTSQLSGGSASSVALPGPGNSKTPVLALPAPD------GTVQA-VN 600
           GMY  G V     TST+  + GSASS+A    G     +LALPAP       G + + V 
Sbjct: 541 GMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTANGNAGNINSPVP 595

Query: 601 QDPFAASLSVPPPSYVQMVEMEKKQHLLTQEQQLWQQYARDGMQGQSSLNKISNPPGYY 626
            DPFAASL V PP+YVQM +MEKKQ +L +EQ +W QY+RDG QG  +L +  N P  Y
Sbjct: 601 MDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHMNLRQNQNQPYSY 595

BLAST of Lsi04G021260 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 451.4 bits (1160), Expect = 1.2e-126
Identity = 300/683 (43.92%), Postives = 398/683 (58.27%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVA--SNMAPDLEVAIVKATSHDDDPASEKYIREILSL 60
           M  S +++AIGAVKDQTS+G+AKV   S+   +LE+A+VKAT HDD PA +KYIREIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR YVSACV+ +S+RL KT++W VALK LIL+ RLL +GD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDF 180
           LNMSDF+D + S SWD+SAFVRTYA YLD+RL                  D R  GR   
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERL------------------DYRMQGR--- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGEGQGRNNKGPVT---P 240
                          RG+++ G  GG       GD GE     G      +K  V    P
Sbjct: 181 ---------------RGKKKSGGGGG-----GDGDSGEEDDHRGTSNDIRSKAIVVKSKP 240

Query: 241 LREMTIERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVL 300
           + EM  E++F ++ HLQ+LLDRFL+CRPTG AKN+R+++ A+YP+V+ESFQLY +I E++
Sbjct: 241 VAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAMYPIVKESFQLYYNITEIM 300

Query: 301 AVLLDKFFDMEYSDCMKAFDAYGSSAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKL 360
            VL+++F +++  D +K ++ +   +KQ DEL  FY WCK+  VARSSEYPE+++IT K 
Sbjct: 301 GVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARSSEYPELEKITQKK 360

Query: 361 LETLEEFLRER----GKRPKSPEREPPPPAPEE------EEPVPDMNEIKALPPPENYTP 420
           L+ ++EF+R++     +  KS  +       EE      +E   D+N IKALP PE    
Sbjct: 361 LDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQEDLNSIKALPAPEQKEE 420

Query: 421 PPPEPEPQPAPKPQPQVTE-----DLVNLRDDA-VSADDQGNKLALALFAGPAA--NGAN 480
              E + +     +  V+      DL++L D+A V+A   G+ LALALF G     + + 
Sbjct: 421 EEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALALFDGVVGTESASG 480

Query: 481 GSWEAFPSDGQPEVTSAWQNPAAESGKADWELALVETASNLSRQKAALGGGLDPLLLNGM 540
             WEAF                     ADWE  LV +A+ LS QK+ LGGG D LLL+GM
Sbjct: 481 PGWEAF-----------------NDNSADWETDLVRSATRLSGQKSELGGGFDTLLLDGM 540

Query: 541 YDQGMVRQ--HTSTSQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQA-----VNQDPF 600
           Y  G V     TST+  S GSASSVA    G+    +LALPAP  T        V  DPF
Sbjct: 541 YQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTANGNRNSPVMVDPF 600

Query: 601 AASLSVPPPSYVQMVEMEKKQHLLTQEQQLWQQYARDGMQGQSSLNKISNPPGYYNTAVA 654
           AASL V PP+YVQM +MEKKQ LL +EQ +W QY R G QG  +  + +    YY     
Sbjct: 601 AASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQGHMNFGQ-NQQQQYYQ---- 610

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LF207.3e-27076.90Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8S9J89.5e-26275.64Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SA654.3e-12944.92Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX471.7e-12543.92Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9ZVN68.0e-6738.69Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE... [more]
Match NameE-valueIdentityDescription
A0A1S3CRD10.0e+0098.01putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0LJA40.0e+0097.24ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G877670 PE=4 S... [more]
A0A6J1IL730.0e+0094.79putative clathrin assembly protein At2g25430 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F9900.0e+0094.79probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1FFH40.0e+0094.33probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_008466416.10.0e+0098.01PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo][more]
XP_004136352.10.0e+0097.24putative clathrin assembly protein At2g25430 [Cucumis sativus] >KGN60096.1 hypot... [more]
XP_038898099.10.0e+0097.09putative clathrin assembly protein At2g25430 [Benincasa hispida][more]
KAG6591568.10.0e+0094.95putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022976123.10.0e+0094.79putative clathrin assembly protein At2g25430 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G25430.15.2e-27176.90epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT4G32285.16.8e-26375.64ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.26.8e-26375.64ENTH/ANTH/VHS superfamily protein [more]
AT1G03050.13.0e-13044.92ENTH/ANTH/VHS superfamily protein [more]
AT4G02650.11.2e-12643.92ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 30..160
e-value: 2.7E-49
score: 179.8
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 24..160
score: 34.682625
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 2..155
e-value: 6.3E-44
score: 151.2
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 30..151
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 227..372
e-value: 4.1E-42
score: 145.3
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 31..365
e-value: 2.3E-84
score: 282.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 160..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 525..549
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 525..545
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 160..193
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..421
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..382
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 5..629
NoneNo IPR availablePANTHERPTHR22951:SF84SUBFAMILY NOT NAMEDcoord: 5..629
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 32..152
e-value: 7.44603E-69
score: 218.262
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 235..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G021260.1Lsi04G021260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048268 clathrin coat assembly
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0005543 phospholipid binding