Lsi04G021240 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G021240
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Auxin-responsive protein
Location	chr04: 28376829 .. 28379276 (+)
RNA-Seq Expression	Lsi04G021240
Synteny	Lsi04G021240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGTACTTGACCTACTTAATTTATGATATGTTTCTACAGAAATGACTATATGACCAAACTTGCATCCATTAAGTAGTATTTTTCTTTGAACGTGTCCATATCACTGCCCTTCTTTCAGCAGACCCAACTTCCATTGTTGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCTACCTAGTTGTCGTCTCCTTCACTCTCTTATCCACACTGATACACGTATAAATAGTCCAAAGTTGCAAACAAAACTACCCATTCTATCCACTGCTAGTGCTAGCCAGCATCATCTCATTGCTTTTGAATTATTTGAAAACAGGAAAGATTCTAATGGAGCTTCAACTTGAGCTAGCGCTTTCTGTACCAGATCCCACCAGAGGTTTTGACCTGAACACGAATGCGTGTGATGGAAAAGATGTGTTTGGTGCAGATCTGAGGACCTGTCTGTGTGTAGAAAGCACGAGCCATGGTAGACACAAGCATGGCTTTGAAAATGCTTTCTTTACGAGCAAAGACTCGTTTAAAGAGTTGTCTTTGCTGCTGTGGAATGGTCACCCAAACAAGGAAGATGATGACAGGAAGGACACCAACCAAAGAAGCTCTTGTGCCATACACATGTGAGTTCAATTCAGACAACTGATTTTTTCCTTTTCTCTTTCTTAAAATATTATTATGTAGGGAGCCAATTTGACATAGCTTAGTGGATAAAGATACCAAGTATTATCCTAAAGGTCGATGGTTTGATCCTCCATCTCAAAATTGTTGAACTCGAAAAAGTATTATTATGTAGGGTGGGAATTTGAACATCAACTCATTAGGGTAGAGGCTAGTATCTAACTACTTTAGTTATGTTCGAATAGGCATTTACATAATTGATGTACTTCAAACAAGAAAACATATTTAGAGGGTTACATAATTGATGTACTTCAAACAAGAAAACATATTTAGAGGGAAAAGTTCATGGTTCAAATAAAATACGAACCCACTCTTTTTTTAAAGAAAATAATATATCTCTGTTCTTAAGTTAGACTTTAGTTCTTATTTTCAATTATGGGCCTGACTTTCATTTGCCTGACTTTCATTTTCAAACTTAAAATATAGTACGGACAACTCTAAGGCTATGGTGCATTTGTTCTGTCACCAAGCATTTGGCCTGATGATTTGTTTCCACTTTTCATAGCAGTAATGCTGGAGAGGAGAAGAAAGTTGTGGGGTGGCCACCCATTAAATCATGGAGGAAAAAGCACCTTCATGGGCTGCAACAGGACGGCCTGGGTTCTGATAATTGTTGGATGGAAGAAGATGAAGATGATGGAATTGTTTTTAACCCAAAATATGTGAAGGTGAAGATGGAGGGGGTACCAATAGCAAGGAAGATTGATGTGGGGTTGTATAACTCATATCAGACGCTGAAAACTGCACTGATCAACATGTTTTCCAATTCTTGCTGTGAGTGAACCATGCAATACTAGTTTCTCTTCCCAAATTGATCTCATAAGGCACAGTTTTTGTTTTTGTTTTTCTATTTTTGAAAACCAAGCCTTCTCCTGGTGTTCTCTTGCCAAAGGATTCAAGGGGTTCTCCATTCAAGATATTAATCGTAGTTGGGAGAATTTTATTGTTCCTTATTGATCTTTTATTGTTCTCTTATTTTTTCCTTATGTTTCTTCTGTTACTGTTGCTGTTTGGATTTGTTCTGTTCTGGTCTTTATGAGTTTTCTTTGTTAACATTGTATCTTGGAGTATTTTTTCTTTTTTCATTCCCTTAATGAAAAGTGTGATATATTTTTTTAAAAAAAAAAACGAAACCAAGTCTTCTCTAATCTAATTTCAAAATGGTTTGTGTTTGCTTTTAAAGTTTGACTAAGAAGTCAAAATTTTCTTTAGAGAGTGAAATACTAAGTAAGGATAACATAGATATATATAGATTTAAAACAGTGAATCAAAATCGTGAGGGGACATGAGGATGACAAAAGAAAAGAAAGCCATAATTATCCAGTTTTTCGAGCCATCTTGAGCCTAACTTAATTGTTTAGGCATATATCCTTGTCTAAGAGGTTAGAGGTTCGGAACCTCCAACCTCACATGTTTTGGAACTCAATGCCAGGTAATTAAATTAGAAACTTGATGTTTTCTTGTGAACAGATCAAAAGTGTGGGAATAGTAATTCCAGCTTAAGTCTCACCTATCAGGACAAAGAAGGAGATTGGCTGCTTGCAGGAGATCTTCCATGGCCGTAAGCACTGTTTCTTTCTTTCTTTATTCCGTCTCTTGTTAAAAATATTGGCAATAGTACAAATCTGAGCTGCAAGTTCAAAGTTTATGATTTTTACAAAGATTCTATGCATTATTAGAACTTAGAAGTAAAAATGATGAACTTTTATTCTATGATATGATGATTTAGATTCTGAATACTTTTACAGGACTTTTCTAGAGTCAGTTCAGTGCATGAAG

mRNA sequence

AGGTACTTGACCTACTTAATTTATGATATGTTTCTACAGAAATGACTATATGACCAAACTTGCATCCATTAAGTAGTATTTTTCTTTGAACGTGTCCATATCACTGCCCTTCTTTCAGCAGACCCAACTTCCATTGTTGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCTACCTAGTTGTCGTCTCCTTCACTCTCTTATCCACACTGATACACGTATAAATAGTCCAAAGTTGCAAACAAAACTACCCATTCTATCCACTGCTAGTGCTAGCCAGCATCATCTCATTGCTTTTGAATTATTTGAAAACAGGAAAGATTCTAATGGAGCTTCAACTTGAGCTAGCGCTTTCTGTACCAGATCCCACCAGAGGTTTTGACCTGAACACGAATGCGTGTGATGGAAAAGATGTGTTTGGTGCAGATCTGAGGACCTGTCTGTGTGTAGAAAGCACGAGCCATGGTAGACACAAGCATGGCTTTGAAAATGCTTTCTTTACGAGCAAAGACTCGTTTAAAGAGTTGTCTTTGCTGCTGTGGAATGGTCACCCAAACAAGGAAGATGATGACAGGAAGGACACCAACCAAAGAAGCTCTTGTGCCATACACATTAATGCTGGAGAGGAGAAGAAAGTTGTGGGGTGGCCACCCATTAAATCATGGAGGAAAAAGCACCTTCATGGGCTGCAACAGGACGGCCTGGGTTCTGATAATTGTTGGATGGAAGAAGATGAAGATGATGGAATTGTTTTTAACCCAAAATATGTGAAGGTGAAGATGGAGGGGGTACCAATAGCAAGGAAGATTGATGTGGGGTTGTATAACTCATATCAGACGCTGAAAACTGCACTGATCAACATGTTTTCCAATTCTTGCTGTGAGTGAACCATGCAATACTAGTTTCTCTTCCCAAATTGATCTCATAAGGCACAGTTTTTGTTTTTGTTTTTCTATTTTTGAAAACCAAGCCTTCTCCTGGTGTTCTCTTGCCAAAGGATTCAAGGGGTTCTCCATTCAAGATATTAATCGTAGTTGGGAGAATTTTATTGTTCCTTATTGATCTTTTATTGTTCTCTTATTTTTTCCTTATGTTTCTTCTGTTACTGTTGCTGTTTGGATTTGTTCTGTTCTGGTCTTTATGAGTTTTCTTTGTTAACATTGTATCTTGGAGTATTTTTTCTTTTTTCATTCCCTTAATGAAAAGTGTGATATATTTTTTTAAAAAAAAAAACGAAACCAAGTCTTCTCTAATCTAATTTCAAAATGGTTTGTGTTTGCTTTTAAAGTTTGACTAAGAAGTCAAAATTTTCTTTAGAGAGTGAAATACTAAGTAAGGATAACATAGATATATATAGATTTAAAACAGTGAATCAAAATCGTGAGGGGACATGAGGATGACAAAAGAAAAGAAAGCCATAATTATCCAGTTTTTCGAGCCATCTTGAGCCTAACTTAATTGTTTAGGCATATATCCTTGTCTAAGAGGTTAGAGGTTCGGAACCTCCAACCTCACATGTTTTGGAACTCAATGCCAGGTAATTAAATTAGAAACTTGATGTTTTCTTGTGAACAGATCAAAAGTGTGGGAATAGTAATTCCAGCTTAAGTCTCACCTATCAGGACAAAGAAGGAGATTGGCTGCTTGCAGGAGATCTTCCATGGCCGTAAGCACTGTTTCTTTCTTTCTTTATTCCGTCTCTTGTTAAAAATATTGGCAATAGTACAAATCTGAGCTGCAAGTTCAAAGTTTATGATTTTTACAAAGATTCTATGCATTATTAGAACTTAGAAGTAAAAATGATGAACTTTTATTCTATGATATGATGATTTAGATTCTGAATACTTTTACAGGACTTTTCTAGAGTCAGTTCAGTGCATGAAG

Coding sequence (CDS)

ATGGAGCTTCAACTTGAGCTAGCGCTTTCTGTACCAGATCCCACCAGAGGTTTTGACCTGAACACGAATGCGTGTGATGGAAAAGATGTGTTTGGTGCAGATCTGAGGACCTGTCTGTGTGTAGAAAGCACGAGCCATGGTAGACACAAGCATGGCTTTGAAAATGCTTTCTTTACGAGCAAAGACTCGTTTAAAGAGTTGTCTTTGCTGCTGTGGAATGGTCACCCAAACAAGGAAGATGATGACAGGAAGGACACCAACCAAAGAAGCTCTTGTGCCATACACATTAATGCTGGAGAGGAGAAGAAAGTTGTGGGGTGGCCACCCATTAAATCATGGAGGAAAAAGCACCTTCATGGGCTGCAACAGGACGGCCTGGGTTCTGATAATTGTTGGATGGAAGAAGATGAAGATGATGGAATTGTTTTTAACCCAAAATATGTGAAGGTGAAGATGGAGGGGGTACCAATAGCAAGGAAGATTGATGTGGGGTTGTATAACTCATATCAGACGCTGAAAACTGCACTGATCAACATGTTTTCCAATTCTTGCTGTGAGTGA

Protein sequence

MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTSKDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLHGLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTALINMFSNSCCE

Homology

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BLAST of Lsi04G021240 vs. ExPASy Swiss-Prot
Match: Q93WC4 (Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 1.4e-11
Identity = 64/211 (30.33%), Postives = 94/211 (44.55%), Query Frame = 0


Query: 1   MELQLELALSVPDPTR---GFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAF 60
           MEL L L+LS    ++    FDLN +      + GA   +CL       G  K  FE  F
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCA----IEGA--ASCL-------GTEKLRFEATF 60

Query: 61  ----FTSKDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIK-- 120
                       +  L   NG PN+ED+D  ++    S  ++ +  E  +VVGWPP+K  
Sbjct: 61  GLGNVEENCYMPKQRLFALNGQPNEEDEDPLES---ESSIVYDDEEENSEVVGWPPVKTC 120

Query: 121 -----SWRKKHL---HGLQQDGLGSDNCWMEEDEDDGIVF-------------NPKYVKV 180
                S+  +H+   H       G     M  +  +                 +  YVKV
Sbjct: 121 MIKYGSYHHRHIRNHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYVKV 180

Query: 181 KMEGVPIARKIDVGLYNSYQTLKTALINMFS 182
           KM+GV IARK+D+ L+NSY++L  +LI MF+
Sbjct: 181 KMDGVAIARKVDIKLFNSYESLTNSLITMFT 195

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BLAST of Lsi04G021240 vs. ExPASy Swiss-Prot
Match: A2WTQ5 (Auxin-responsive protein IAA5 OS=Oryza sativa subsp. indica OX=39946 GN=IAA5 PE=2 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 5.7e-10
Identity = 33/79 (41.77%), Postives = 44/79 (55.70%), Query Frame = 0


Query: 103 KVVGWPPIKSWRKKHLHGLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKID 162
           +VVGWPP++++RK  L      G G D    E          P YVKV M+G P  RK+D
Sbjct: 118 QVVGWPPVRNYRKNTLAASASKGKGEDKGTAEG--------GPLYVKVSMDGAPYLRKVD 177

Query: 163 VGLYNSYQTLKTALINMFS 182
           + +Y+SY+ L  AL  MFS
Sbjct: 178 LKMYSSYEDLSMALEKMFS 188

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BLAST of Lsi04G021240 vs. ExPASy Swiss-Prot
Match: Q0JKG7 (Auxin-responsive protein IAA5 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA5 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.7e-10
Identity = 33/79 (41.77%), Postives = 44/79 (55.70%), Query Frame = 0


Query: 103 KVVGWPPIKSWRKKHLHGLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKID 162
           +VVGWPP++++RK  L      G G D    E          P YVKV M+G P  RK+D
Sbjct: 117 QVVGWPPVRNYRKNTLAASASKGKGEDKGTAEG--------GPLYVKVSMDGAPYLRKVD 176

Query: 163 VGLYNSYQTLKTALINMFS 182
           + +Y+SY+ L  AL  MFS
Sbjct: 177 LKMYSSYEDLSMALEKMFS 187

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BLAST of Lsi04G021240 vs. ExPASy Swiss-Prot
Match: Q9ZSY8 (Auxin-responsive protein IAA27 OS=Arabidopsis thaliana OX=3702 GN=IAA27 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.7e-10
Identity = 35/79 (44.30%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 103 KVVGWPPIKSWRKKHLHGLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKID 162
           +VVGWPPI+S+RK  +   Q    G+++   E +   G      YVKV MEG P  RKID
Sbjct: 143 QVVGWPPIRSFRKNSMASSQSQKPGNNSETEEAEAKSGPEQPCLYVKVSMEGAPYLRKID 202

Query: 163 VGLYNSYQTLKTALINMFS 182
           +  Y SY  L +AL  MFS
Sbjct: 203 LKTYKSYLELSSALEKMFS 221

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BLAST of Lsi04G021240 vs. ExPASy Swiss-Prot
Match: P93830 (Auxin-responsive protein IAA17 OS=Arabidopsis thaliana OX=3702 GN=IAA17 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 2.8e-09
Identity = 42/123 (34.15%), Postives = 60/123 (48.78%), Query Frame = 0


Query: 66  ELSLLLWNGHPNKE-----DDDRKDTNQRSSCAIH-INAGEEKKVVGWPPIKSWRKKHLH 125
           +L L L N   NKE     D    D+ ++S+C         + +VVGWPP++S+RK  + 
Sbjct: 39  DLKLNLNNEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMV 98

Query: 126 GLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTALINM 183
             Q+   G +                 +VKV M+G P  RKID+ +Y SY  L  AL NM
Sbjct: 99  SCQKSSGGPEAA--------------AFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNM 147

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BLAST of Lsi04G021240 vs. ExPASy TrEMBL
Match: A0A0A0LGM4 (Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_3G877650 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.6e-87
Identity = 158/185 (85.41%), Postives = 167/185 (90.27%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHG-RHKHGFENAFFT 60
           MELQLEL LSVPDPTRGFDLN NACDGKDVFG+D R+ LC ESTSHG R+K GFE+AFF 
Sbjct: 1   MELQLELELSVPDPTRGFDLNRNACDGKDVFGSDPRSYLCAESTSHGKRNKRGFEDAFFK 60

Query: 61  SKDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLH 120
           +K SFKE+SLLLWNGHPNKEDDDRKDTNQRSSCAIHI A EE K VGWPPI SWRK+HLH
Sbjct: 61  TKGSFKEMSLLLWNGHPNKEDDDRKDTNQRSSCAIHIKAVEENKAVGWPPIASWRKRHLH 120

Query: 121 GLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTALINM 180
           GLQQ G GSDN WMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVG+YNSYQTLKTA INM
Sbjct: 121 GLQQGGPGSDNYWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGMYNSYQTLKTASINM 180

Query: 181 FSNSC 185
           FS+SC
Sbjct: 181 FSDSC 185

[Close]

BLAST of Lsi04G021240 vs. ExPASy TrEMBL
Match: A0A6J1FF02 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 6.0e-87
Identity = 157/188 (83.51%), Postives = 170/188 (90.43%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MELQLELAL V DPTRGFDLN  ACDGKDVFG+D R+CLC ESTSHGRHK G+E+AFF S
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLHG 120
           KDSFKELSLLLW+GHPNKEDDD KDTNQRSSCA+HINAGEE KV+GWPPIKSWRKK+LH 
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHINAGEENKVMGWPPIKSWRKKYLHE 120

Query: 121 LQQDGLGSD----NCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTAL 180
           LQQDG GSD    + WMEEDEDD IVFNPKYVKVKMEGVPIARKIDVGLY+SYQTL+TAL
Sbjct: 121 LQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLETAL 180

Query: 181 INMFSNSC 185
           I+MFS+SC
Sbjct: 181 IHMFSDSC 188

[Close]

BLAST of Lsi04G021240 vs. ExPASy TrEMBL
Match: A0A6J1ILE5 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.3e-86
Identity = 157/188 (83.51%), Postives = 169/188 (89.89%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MELQLELAL V DPTRGFDLN  ACDGKDVFG+D R+CLC ESTSHGRHK G+E+AFF S
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLHG 120
           KDSFKELSLLLW+GHPNKEDDD KDTNQRSSCA+HINAGEE KV+GWPPIKSWRKK+LH 
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHINAGEENKVMGWPPIKSWRKKYLHE 120

Query: 121 LQQDGLGSD----NCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTAL 180
           LQQDG GSD    + WMEEDED+ IVFNPKYVKVKMEGVPIARKIDVGLY+SYQTLKTAL
Sbjct: 121 LQQDGFGSDQIPNHYWMEEDEDEEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTAL 180

Query: 181 INMFSNSC 185
           I MFS+SC
Sbjct: 181 IPMFSDSC 188

[Close]

BLAST of Lsi04G021240 vs. ExPASy TrEMBL
Match: A0A6J1FA34 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.5e-85
Identity = 157/189 (83.07%), Postives = 170/189 (89.95%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MELQLELAL V DPTRGFDLN  ACDGKDVFG+D R+CLC ESTSHGRHK G+E+AFF S
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHI-NAGEEKKVVGWPPIKSWRKKHLH 120
           KDSFKELSLLLW+GHPNKEDDD KDTNQRSSCA+HI NAGEE KV+GWPPIKSWRKK+LH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 GLQQDGLGSD----NCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTA 180
            LQQDG GSD    + WMEEDEDD IVFNPKYVKVKMEGVPIARKIDVGLY+SYQTL+TA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLETA 180

Query: 181 LINMFSNSC 185
           LI+MFS+SC
Sbjct: 181 LIHMFSDSC 189

[Close]

BLAST of Lsi04G021240 vs. ExPASy TrEMBL
Match: A0A6J1IF49 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.3e-85
Identity = 157/189 (83.07%), Postives = 169/189 (89.42%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MELQLELAL V DPTRGFDLN  ACDGKDVFG+D R+CLC ESTSHGRHK G+E+AFF S
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHI-NAGEEKKVVGWPPIKSWRKKHLH 120
           KDSFKELSLLLW+GHPNKEDDD KDTNQRSSCA+HI NAGEE KV+GWPPIKSWRKK+LH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 GLQQDGLGSD----NCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTA 180
            LQQDG GSD    + WMEEDED+ IVFNPKYVKVKMEGVPIARKIDVGLY+SYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDEEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LINMFSNSC 185
           LI MFS+SC
Sbjct: 181 LIPMFSDSC 189

[Close]

BLAST of Lsi04G021240 vs. NCBI nr
Match: XP_038896899.1 (auxin-responsive protein IAA29-like [Benincasa hispida])

HSP 1 Score: 357.1 bits (915), Expect = 9.4e-95
Identity = 166/184 (90.22%), Postives = 173/184 (94.02%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MEL+LELALSVPDPTRGFDLN N CDGKDVFG D R+CLC ESTSHGRHK GFE+AFF S
Sbjct: 1   MELELELALSVPDPTRGFDLNRNVCDGKDVFGLDTRSCLCAESTSHGRHKRGFEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLHG 120
           KDSFKELSLLLW+GHPNKEDDDRKDT+QRSSCAIHINAGE  KVVGWPPIKSWRKKHLHG
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDRKDTDQRSSCAIHINAGENNKVVGWPPIKSWRKKHLHG 120

Query: 121 LQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTALINMF 180
           LQQDG GSDN WMEEDEDDGIVFNPKYVKVKMEG+PIARKIDVGLYNSYQTLKTALI+MF
Sbjct: 121 LQQDGPGSDNYWMEEDEDDGIVFNPKYVKVKMEGMPIARKIDVGLYNSYQTLKTALIDMF 180

Query: 181 SNSC 185
           SNSC
Sbjct: 181 SNSC 184

[Close]

BLAST of Lsi04G021240 vs. NCBI nr
Match: KAG7024457.1 (Auxin-responsive protein IAA27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 332.4 bits (851), Expect = 2.5e-87
Identity = 160/191 (83.77%), Postives = 172/191 (90.05%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MELQLELAL V DPTRGFDLN  ACDGKDVFG+D R+CLC ESTSHGRHK G+E+AFF S
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHI-NAGEEKKVVGWPPIKSWRKKHLH 120
           KDSFKELSLLLW+GHPNKEDDD KDTNQRSSCA+HI NAGEE KV+GWPPIKSWRKK+LH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 GLQQDGLGSD----NCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTA 180
            LQQDG GSD    + WMEEDEDD IVFNPKYVKVKMEGVPIARKIDVGLY+SYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LINMFSNSCCE 187
           LI+MFS+SCCE
Sbjct: 181 LIHMFSDSCCE 191

[Close]

BLAST of Lsi04G021240 vs. NCBI nr
Match: XP_004136353.1 (auxin-responsive protein IAA20 [Cucumis sativus] >KGN60094.1 hypothetical protein Csa_001988 [Cucumis sativus])

HSP 1 Score: 332.0 bits (850), Expect = 3.2e-87
Identity = 158/185 (85.41%), Postives = 167/185 (90.27%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHG-RHKHGFENAFFT 60
           MELQLEL LSVPDPTRGFDLN NACDGKDVFG+D R+ LC ESTSHG R+K GFE+AFF 
Sbjct: 1   MELQLELELSVPDPTRGFDLNRNACDGKDVFGSDPRSYLCAESTSHGKRNKRGFEDAFFK 60

Query: 61  SKDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLH 120
           +K SFKE+SLLLWNGHPNKEDDDRKDTNQRSSCAIHI A EE K VGWPPI SWRK+HLH
Sbjct: 61  TKGSFKEMSLLLWNGHPNKEDDDRKDTNQRSSCAIHIKAVEENKAVGWPPIASWRKRHLH 120

Query: 121 GLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTALINM 180
           GLQQ G GSDN WMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVG+YNSYQTLKTA INM
Sbjct: 121 GLQQGGPGSDNYWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGMYNSYQTLKTASINM 180

Query: 181 FSNSC 185
           FS+SC
Sbjct: 181 FSDSC 185

[Close]

BLAST of Lsi04G021240 vs. NCBI nr
Match: KAG6591567.1 (Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia] >KAG6591604.1 Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 331.6 bits (849), Expect = 4.2e-87
Identity = 158/188 (84.04%), Postives = 170/188 (90.43%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MELQLELAL V DPTRGFDLN  ACDGKDVFG+D R+CLC ESTSHGRHK G+E+AFF S
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLHG 120
           KDSFKELSLLLW+GHPNKEDDD KDTNQRSSCA+HINAGEE KV+GWPPIKSWRKK+LH 
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHINAGEENKVMGWPPIKSWRKKYLHE 120

Query: 121 LQQDGLGSD----NCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTAL 180
           LQQDG GSD    + WMEEDEDD IVFNPKYVKVKMEGVPIARKIDVGLY+SYQTLKTAL
Sbjct: 121 LQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTAL 180

Query: 181 INMFSNSC 185
           I+MFS+SC
Sbjct: 181 IHMFSDSC 188

[Close]

BLAST of Lsi04G021240 vs. NCBI nr
Match: XP_022937068.1 (auxin-responsive protein IAA28 isoform X2 [Cucurbita moschata])

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-86
Identity = 157/188 (83.51%), Postives = 170/188 (90.43%), Query Frame = 0


Query: 1   MELQLELALSVPDPTRGFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAFFTS 60
           MELQLELAL V DPTRGFDLN  ACDGKDVFG+D R+CLC ESTSHGRHK G+E+AFF S
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIKSWRKKHLHG 120
           KDSFKELSLLLW+GHPNKEDDD KDTNQRSSCA+HINAGEE KV+GWPPIKSWRKK+LH 
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHINAGEENKVMGWPPIKSWRKKYLHE 120

Query: 121 LQQDGLGSD----NCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTAL 180
           LQQDG GSD    + WMEEDEDD IVFNPKYVKVKMEGVPIARKIDVGLY+SYQTL+TAL
Sbjct: 121 LQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLETAL 180

Query: 181 INMFSNSC 185
           I+MFS+SC
Sbjct: 181 IHMFSDSC 188

[Close]

BLAST of Lsi04G021240 vs. TAIR 10
Match: AT4G32280.1 (indole-3-acetic acid inducible 29 )

HSP 1 Score: 71.2 bits (173), Expect = 9.6e-13
Identity = 64/211 (30.33%), Postives = 94/211 (44.55%), Query Frame = 0


Query: 1   MELQLELALSVPDPTR---GFDLNTNACDGKDVFGADLRTCLCVESTSHGRHKHGFENAF 60
           MEL L L+LS    ++    FDLN +      + GA   +CL       G  K  FE  F
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCA----IEGA--ASCL-------GTEKLRFEATF 60

Query: 61  ----FTSKDSFKELSLLLWNGHPNKEDDDRKDTNQRSSCAIHINAGEEKKVVGWPPIK-- 120
                       +  L   NG PN+ED+D  ++    S  ++ +  E  +VVGWPP+K  
Sbjct: 61  GLGNVEENCYMPKQRLFALNGQPNEEDEDPLES---ESSIVYDDEEENSEVVGWPPVKTC 120

Query: 121 -----SWRKKHL---HGLQQDGLGSDNCWMEEDEDDGIVF-------------NPKYVKV 180
                S+  +H+   H       G     M  +  +                 +  YVKV
Sbjct: 121 MIKYGSYHHRHIRNHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYVKV 180

Query: 181 KMEGVPIARKIDVGLYNSYQTLKTALINMFS 182
           KM+GV IARK+D+ L+NSY++L  +LI MF+
Sbjct: 181 KMDGVAIARKVDIKLFNSYESLTNSLITMFT 195

[Close]

BLAST of Lsi04G021240 vs. TAIR 10
Match: AT4G29080.1 (phytochrome-associated protein 2 )

HSP 1 Score: 65.1 bits (157), Expect = 6.9e-11
Identity = 35/79 (44.30%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 103 KVVGWPPIKSWRKKHLHGLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKID 162
           +VVGWPPI+S+RK  +   Q    G+++   E +   G      YVKV MEG P  RKID
Sbjct: 143 QVVGWPPIRSFRKNSMASSQSQKPGNNSETEEAEAKSGPEQPCLYVKVSMEGAPYLRKID 202

Query: 163 VGLYNSYQTLKTALINMFS 182
           +  Y SY  L +AL  MFS
Sbjct: 203 LKTYKSYLELSSALEKMFS 221

[Close]

BLAST of Lsi04G021240 vs. TAIR 10
Match: AT1G04250.1 (AUX/IAA transcriptional regulator family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-10
Identity = 42/123 (34.15%), Postives = 60/123 (48.78%), Query Frame = 0


Query: 66  ELSLLLWNGHPNKE-----DDDRKDTNQRSSCAIH-INAGEEKKVVGWPPIKSWRKKHLH 125
           +L L L N   NKE     D    D+ ++S+C         + +VVGWPP++S+RK  + 
Sbjct: 39  DLKLNLNNEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMV 98

Query: 126 GLQQDGLGSDNCWMEEDEDDGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLKTALINM 183
             Q+   G +                 +VKV M+G P  RKID+ +Y SY  L  AL NM
Sbjct: 99  SCQKSSGGPEAA--------------AFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNM 147

[Close]

BLAST of Lsi04G021240 vs. TAIR 10
Match: AT3G23050.2 (indole-3-acetic acid 7 )

HSP 1 Score: 61.2 bits (147), Expect = 9.9e-10
Identity = 42/129 (32.56%), Postives = 67/129 (51.94%), Query Frame = 0


Query: 60  SKDSFKELSLLLWNGHPNKEDD-DRKDTN----QRSSCAIHINAGEEKKVVGWPPIKSWR 119
           SK  F E   L+ N   NKE   D K+ +    ++++         + +VVGWPP++++R
Sbjct: 34  SKRGFSETVDLMLNLQSNKEGSVDLKNVSAVPKEKTTLKDPSKPPAKAQVVGWPPVRNYR 93

Query: 120 KKHLHGLQQDGLGSDNCWMEEDED-DGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLK 179
            K++   Q+   G++    E+  +  G       VKV M+G P  RK+D+ +Y SYQ L 
Sbjct: 94  -KNMMTQQKTSSGAEEASSEKAGNFGGGAAGAGLVKVSMDGAPYLRKVDLKMYKSYQDLS 153

Query: 180 TALINMFSN 183
            AL  MFS+
Sbjct: 154 DALAKMFSS 161

[Close]

BLAST of Lsi04G021240 vs. TAIR 10
Match: AT3G23050.1 (indole-3-acetic acid 7 )

HSP 1 Score: 61.2 bits (147), Expect = 9.9e-10
Identity = 42/129 (32.56%), Postives = 67/129 (51.94%), Query Frame = 0


Query: 60  SKDSFKELSLLLWNGHPNKEDD-DRKDTN----QRSSCAIHINAGEEKKVVGWPPIKSWR 119
           SK  F E   L+ N   NKE   D K+ +    ++++         + +VVGWPP++++R
Sbjct: 34  SKRGFSETVDLMLNLQSNKEGSVDLKNVSAVPKEKTTLKDPSKPPAKAQVVGWPPVRNYR 93

Query: 120 KKHLHGLQQDGLGSDNCWMEEDED-DGIVFNPKYVKVKMEGVPIARKIDVGLYNSYQTLK 179
            K++   Q+   G++    E+  +  G       VKV M+G P  RK+D+ +Y SYQ L 
Sbjct: 94  -KNMMTQQKTSSGAEEASSEKAGNFGGGAAGAGLVKVSMDGAPYLRKVDLKMYKSYQDLS 153

Query: 180 TALINMFSN 183
            AL  MFS+
Sbjct: 154 DALAKMFSS 161

The following BLAST results are available for this feature:

BLAST of Lsi04G021240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WC4	1.4e-11	30.33	Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=...	[more]
A2WTQ5	5.7e-10	41.77	Auxin-responsive protein IAA5 OS=Oryza sativa subsp. indica OX=39946 GN=IAA5 PE=...	[more]
Q0JKG7	5.7e-10	41.77	Auxin-responsive protein IAA5 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA5 P...	[more]
Q9ZSY8	9.7e-10	44.30	Auxin-responsive protein IAA27 OS=Arabidopsis thaliana OX=3702 GN=IAA27 PE=1 SV=...	[more]
P93830	2.8e-09	34.15	Auxin-responsive protein IAA17 OS=Arabidopsis thaliana OX=3702 GN=IAA17 PE=1 SV=...	[more]

BLAST of Lsi04G021240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGM4	1.6e-87	85.41	Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_3G877650 PE=3 SV=1	[more]
A0A6J1FF02	6.0e-87	83.51	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1	[more]
A0A6J1ILE5	1.3e-86	83.51	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1	[more]
A0A6J1FA34	1.5e-85	83.07	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1	[more]
A0A6J1IF49	3.3e-85	83.07	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1	[more]

BLAST of Lsi04G021240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896899.1	9.4e-95	90.22	auxin-responsive protein IAA29-like [Benincasa hispida]	[more]
KAG7024457.1	2.5e-87	83.77	Auxin-responsive protein IAA27 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_004136353.1	3.2e-87	85.41	auxin-responsive protein IAA20 [Cucumis sativus] >KGN60094.1 hypothetical protei...	[more]
KAG6591567.1	4.2e-87	84.04	Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia] ...	[more]
XP_022937068.1	1.2e-86	83.51	auxin-responsive protein IAA28 isoform X2 [Cucurbita moschata]	[more]

BLAST of Lsi04G021240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32280.1	9.6e-13	30.33	indole-3-acetic acid inducible 29	[more]
AT4G29080.1	6.9e-11	44.30	phytochrome-associated protein 2	[more]
AT1G04250.1	2.0e-10	34.15	AUX/IAA transcriptional regulator family protein	[more]
AT3G23050.2	9.9e-10	32.56	indole-3-acetic acid 7	[more]
AT3G23050.1	9.9e-10	32.56	indole-3-acetic acid 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033389	AUX/IAA domain	PFAM	PF02309	AUX_IAA	coord: 46..182 e-value: 1.1E-23 score: 84.4
None	No IPR available	GENE3D	3.10.20.90		coord: 143..186 e-value: 9.1E-11 score: 43.6
None	No IPR available	PANTHER	PTHR31734:SF44	AUXIN-RESPONSIVE PROTEIN IAA29	coord: 1..183
IPR003311	AUX/IAA protein	PANTHER	PTHR31734	AUXIN-RESPONSIVE PROTEIN IAA17	coord: 1..183
IPR000270	PB1 domain	PROSITE	PS51745	PB1	coord: 145..186 score: 15.259787

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G021240.1	Lsi04G021240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding