Lsi04G020880 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G020880
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	PPPDE domain-containing protein
Location	chr04: 28078660 .. 28080527 (+)
RNA-Seq Expression	Lsi04G020880
Synteny	Lsi04G020880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTGTGTTCCTTCTAATTCTCGCTTTTCGGTTTCTCAATCTTCTTCCTCTTTGACCAAGTTCCTTCGGATTGAATCTTATGTGTTGTATGATTTTGATTTCAGAGAGAAAGAGAAGAAGAAGAAGAAAAGAAGTGGTGGGTTTGGGGAAAATGTTGTGCAGAATTGCTATGATGTCGAAGAAGAAGAAGAGTGGAACAGTGCCGGTTTATTTGAATGTATATGATTTAACGCCGATCAATGGCTATGCGTATTGGCTCGGTCTTGGGGTTTACCATTCCGGCGTGCAAGGTTAGACAATTTATGGCGTTTTTTTCTTTCTCTTCTGTGTTTCTTCTTCTTCCCTTTTTGGGATGTTGTTAGCAAAGATGATGAGGATTTGGGTTTCTTGTAAAACATTTTTTCCATATGGACCAAGGGTTTTTTAAATTGTTGTTTCCTTTTTTCTTGATTCTCCATTGAGTTTTCCTTCAGATCTGAGTTGAATTCGTTCTGTTTTTGGCTGAAATTTGTGATACTTGATAAGCTGGAGGAACATGGGGGGGTTTTATGGCAGTGTTGTGAAGTTTTCTGGTTTTGATTGTGTATTTATCTCTGTGAAAAGATGATTCTGTAATGTTTAGCTTGATTTGGCTGAGATATTGATGAATGATGTGTTGAAAAACTTTGAGTTCCTGCATGTTTGTTTGCTTCTAATTGTTTATATGAGTTTCTATTGACCTTAATCTATATCGTTTGTGTTTGACCTTTTTAAGAGTTTTTTGATTGATGACTTAAGCAGTCAATGTTTTTCTGTGATTAAAATCCAAAAGAAATCTCCGATGTATCCAGCTTGTTTAATTTATATTATCAAGTAGTACAGGGTGTTCAAAGTTCAAATATCTTACTGGGAAGAATCTTTTTGCAGTTCATGGAGTTGAATATGCATTTGGAGCACATGAACATGCTACTTCAGGTATATTTGAGGTAGAACCAAGACAATGTCCTGGTTTTACATTTAGGAAATCAATTTGCATTGGAAGAACGAATCTCGGTCCAAAAGATGTTCGATCGTTTATGGAGAAACTAGCTGAAGAGTACTCTGGGAACACTTATCATCTTATAACAAGAAATTGCAACCACTTTTGCAATGATGTATGCTTAAAGTTGGCAGGGAAACCAATCCCTAGCTGGGTCAACCGACTTGCTCGACTTGGTATGATCGCCGGTTATATTCCCAGCTTAACCGTTTTCGTTTCTATAATCATATTCTGTTCTATGCTCAAAATTTTGGTCACCCAACATCCTGTTTGTACTTGATGTGTCATAGAAACCTAGAAGTGAAAGTTTTCATTTAATGGAGCATCACCAGTTTACAAACAAACTGCCCATCATAACATAACCACTGTGTTTCTATGGCTTCCCCTGCATTATTAAGAGTATGAATGATCTGGTATCGAGACGTTAATTATCTAAGTGATACCGTTACGAGCGTTTCATTTGTTCCCTCTACTACCTTCTGCACATGGTTGAGATTGTAACTTACAACAATTTGTTATCTTTAATTTGATATGTTTTCGGTTTTTCAGGTCTTTTCTGCAACTGCGTTCTTCCAGCGAGTTTGAACGAAGCAAAGGTTCGACAAGTAAGATCAGGAGATAAGCTTAATGAAGGTGTAAAGAAGAAATTGAAAAGCCAACCAATTAGGTATAATAATACATCTTCATCTAACCCTCCACCACCACCCTTACCTTCTAATCCATCAACTTCAACCATCAAAGTATGTAGACAAAAACGATGTATCCCGACAGCGTCATCGGTGATTCATTCTTCATCATCCACAACCCTGACTCTGAAGTTGTAACAATAATTGGTTTCTTTGTATAG

mRNA sequence

ATGAATTGTGTTCCTTCTAATTCTCGCTTTTCGGTTTCTCAATCTTCTTCCTCTTTGACCAAGTTCCTTCGGATTGAATCTTATGTGTTAATTGCTATGATGTCGAAGAAGAAGAAGAGTGGAACAGTGCCGGTTTATTTGAATGTATATGATTTAACGCCGATCAATGGCTATGCGTATTGGCTCGGTCTTGGGGTTTACCATTCCGGCGTGCAAGTTCATGGAGTTGAATATGCATTTGGAGCACATGAACATGCTACTTCAGGTATATTTGAGGTAGAACCAAGACAATGTCCTGGTTTTACATTTAGGAAATCAATTTGCATTGGAAGAACGAATCTCGGTCCAAAAGATGTTCGATCGTTTATGGAGAAACTAGCTGAAGAGTACTCTGGGAACACTTATCATCTTATAACAAGAAATTGCAACCACTTTTGCAATGATGTATGCTTAAAGTTGGCAGGGAAACCAATCCCTAGCTGGGTCAACCGACTTGCTCGACTTGGTCTTTTCTGCAACTGCGTTCTTCCAGCGAGTTTGAACGAAGCAAAGGTTCGACAAGTAAGATCAGGAGATAAGCTTAATGAAGGTGTAAAGAAGAAATTGAAAAGCCAACCAATTAGCGTCATCGGTGATTCATTCTTCATCATCCACAACCCTGACTCTGAAGTTGTAACAATAATTGGTTTCTTTGTATAG

Coding sequence (CDS)

ATGAATTGTGTTCCTTCTAATTCTCGCTTTTCGGTTTCTCAATCTTCTTCCTCTTTGACCAAGTTCCTTCGGATTGAATCTTATGTGTTAATTGCTATGATGTCGAAGAAGAAGAAGAGTGGAACAGTGCCGGTTTATTTGAATGTATATGATTTAACGCCGATCAATGGCTATGCGTATTGGCTCGGTCTTGGGGTTTACCATTCCGGCGTGCAAGTTCATGGAGTTGAATATGCATTTGGAGCACATGAACATGCTACTTCAGGTATATTTGAGGTAGAACCAAGACAATGTCCTGGTTTTACATTTAGGAAATCAATTTGCATTGGAAGAACGAATCTCGGTCCAAAAGATGTTCGATCGTTTATGGAGAAACTAGCTGAAGAGTACTCTGGGAACACTTATCATCTTATAACAAGAAATTGCAACCACTTTTGCAATGATGTATGCTTAAAGTTGGCAGGGAAACCAATCCCTAGCTGGGTCAACCGACTTGCTCGACTTGGTCTTTTCTGCAACTGCGTTCTTCCAGCGAGTTTGAACGAAGCAAAGGTTCGACAAGTAAGATCAGGAGATAAGCTTAATGAAGGTGTAAAGAAGAAATTGAAAAGCCAACCAATTAGCGTCATCGGTGATTCATTCTTCATCATCCACAACCCTGACTCTGAAGTTGTAACAATAATTGGTTTCTTTGTATAG

Protein sequence

MNCVPSNSRFSVSQSSSSLTKFLRIESYVLIAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPISVIGDSFFIIHNPDSEVVTIIGFFV

Homology

[Close]

BLAST of Lsi04G020880 vs. ExPASy Swiss-Prot
Match: Q93VG8 (DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.9e-56
Identity = 93/188 (49.47%), Postives = 133/188 (70.74%), Query Frame = 0


Query: 39  KSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEVEPRQC 98
           ++   PVYLNVYDLTP+N Y YW G+G++HSG++ H +EY +GAHE+ TSG++EVEPR C
Sbjct: 22  EAALTPVYLNVYDLTPVNNYLYWFGIGIFHSGIEAHNLEYCYGAHEYPTSGVYEVEPRNC 81

Query: 99  PGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKLAGKPI 158
           PGF FR+S+ +G T++   D RS+MEKL+ +Y G+TYHLI +NCNHF  +VCL+L GKPI
Sbjct: 82  PGFIFRRSVLLGTTSMSRSDFRSYMEKLSRKYHGDTYHLIAKNCNHFTEEVCLQLTGKPI 141

Query: 159 PSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPIS-VIGDSFFII 218
           P W+NRLAR+G FCNC+LP S+    V  +    + ++  +   ++  +S   G    +I
Sbjct: 142 PGWINRLARVGSFCNCLLPESIQLTAVSALPERLEFSDEDESNSEASSVSDEEGSEQHLI 201

Query: 219 HNPDSEVV 226
           +  D E+V
Sbjct: 202 NVADREIV 209

[Close]

BLAST of Lsi04G020880 vs. ExPASy Swiss-Prot
Match: H2KZK4 (Desumoylating isopeptidase 1 homolog OS=Caenorhabditis elegans OX=6239 GN=F36D4.5 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.6e-30
Identity = 68/169 (40.24%), Postives = 97/169 (57.40%), Query Frame = 0


Query: 45  VYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEVEPRQC----PG 104
           V LNVYD+  +N YA  +G+G++HSG++V GVEYA+G H +  SG+FE  P+        
Sbjct: 32  VRLNVYDMYWLNDYASNIGVGIFHSGIEVFGVEYAYGGHPYQFSGVFENSPQDAEELGET 91

Query: 105 FTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKLAGKPIPS 164
           F F++SI +G T     D+R  ++ L E++ G+ YHLI+RNCNHF   +  +L GK IP 
Sbjct: 92  FKFKESIVVGETERSTSDIRKLIKSLGEDFRGDRYHLISRNCNHFSAVLARELTGKDIPG 151

Query: 165 WVNRLARLG-------------LFCNCVLPASLNEAKVRQVRSGDKLNE 197
           W+NRLA L                   VL AS++E K   V S ++  E
Sbjct: 152 WINRLANLSGSIPFLEKCIPQEWLTPIVLQASVDEKKRGSVDSAEEATE 200

[Close]

BLAST of Lsi04G020880 vs. ExPASy Swiss-Prot
Match: Q5PQ09 (Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 8.1e-30
Identity = 62/136 (45.59%), Postives = 86/136 (63.24%), Query Frame = 0


Query: 44  PVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEVEPRQC----P 103
           P+ LNVYD+  IN Y   LG+GV+HSG+QV+G E+A+G H +  SG+FE+ P        
Sbjct: 5   PIILNVYDMYWINEYTSSLGIGVFHSGIQVYGREFAYGGHPYPFSGVFEISPGDSTELGD 64

Query: 104 GFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKLAGKPIP 163
            F F+++I +G T+    D+   +E+L +EY GN YHL+ +NCNHF + +   L GK IP
Sbjct: 65  TFKFKEAIALGSTDFTENDIEKIIEELGKEYKGNAYHLMHKNCNHFSSALSEILCGKEIP 124

Query: 164 SWVNRLARLGLFCNCV 176
            WVNRLA    F  CV
Sbjct: 125 RWVNRLA---YFSTCV 137

[Close]

BLAST of Lsi04G020880 vs. ExPASy Swiss-Prot
Match: Q6DC39 (Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-28
Identity = 60/136 (44.12%), Postives = 88/136 (64.71%), Query Frame = 0


Query: 44  PVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEVEPRQC----P 103
           PV LNVYD+  IN +   LG+GV+HSG++++G E+A+G H +  SGIFE+ P        
Sbjct: 5   PVILNVYDMYWINEFTSSLGIGVFHSGIEIYGREFAYGGHPYPFSGIFEITPGDATELGE 64

Query: 104 GFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKLAGKPIP 163
            F F+++I +G T+   +DV   +E++ +EY GN YHL+ +NCNHF + +   L G+ IP
Sbjct: 65  TFKFKEAIVLGSTDFTEEDVERIVEEMGKEYKGNAYHLMHKNCNHFSSALSEILCGREIP 124

Query: 164 SWVNRLARLGLFCNCV 176
            WVNRLA    F +CV
Sbjct: 125 RWVNRLA---YFSSCV 137

[Close]

BLAST of Lsi04G020880 vs. ExPASy Swiss-Prot
Match: Q5ZIV7 (Deubiquitinase DESI2 OS=Gallus gallus OX=9031 GN=DESI2 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.8e-28
Identity = 58/135 (42.96%), Postives = 86/135 (63.70%), Query Frame = 0


Query: 45  VYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEVEPRQC----PG 104
           V LNVYD+  +N Y   LG+GV+HSG++V+G E+A+G H +  SGIFE+ P         
Sbjct: 6   VILNVYDMYWMNEYTSSLGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNASELGET 65

Query: 105 FTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKLAGKPIPS 164
           F F++++ +G T+    D+   +E+L +E+ GN YHL+ +NCNHF + +   L GK IP 
Sbjct: 66  FKFKEAVVLGSTDFMEDDIEKIVEELGKEFKGNAYHLMHKNCNHFSSALSEILCGKEIPR 125

Query: 165 WVNRLARLGLFCNCV 176
           WVNRLA    F +C+
Sbjct: 126 WVNRLA---YFSSCI 137

[Close]

BLAST of Lsi04G020880 vs. ExPASy TrEMBL
Match: A0A0A0L339 (PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652130 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.2e-99
Identity = 174/177 (98.31%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 5   IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 64

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 65  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 124

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPI 208
           ++LAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPI
Sbjct: 125 IRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPI 181

[Close]

BLAST of Lsi04G020880 vs. ExPASy TrEMBL
Match: A0A5A7VGG1 (DeSI-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001070 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 4.2e-98
Identity = 171/176 (97.16%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 24  IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 83

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPK+VRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 84  FEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 143

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 207
           ++LAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKL+SQP
Sbjct: 144 IRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLRSQP 199

[Close]

BLAST of Lsi04G020880 vs. ExPASy TrEMBL
Match: A0A1S3BVP4 (deSI-like protein At4g17486 OS=Cucumis melo OX=3656 GN=LOC103493768 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 4.2e-98
Identity = 171/176 (97.16%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 5   IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 64

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPK+VRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 65  FEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 124

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 207
           ++LAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKL+SQP
Sbjct: 125 IRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLRSQP 180

[Close]

BLAST of Lsi04G020880 vs. ExPASy TrEMBL
Match: A0A6J1ISX1 (deSI-like protein At4g17486 OS=Cucurbita maxima OX=3661 GN=LOC111480313 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.2e-94
Identity = 163/181 (90.06%), Postives = 172/181 (95.03%), Query Frame = 0


Query: 25  IESYVLIAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHE 84
           +E    +A+MSKKKKSGTVPVYLNVYDLTPINGYAYW GLGVYHSGVQVHGVEYAFGAH+
Sbjct: 2   VEMLRRMALMSKKKKSGTVPVYLNVYDLTPINGYAYWFGLGVYHSGVQVHGVEYAFGAHD 61

Query: 85  HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNH 144
           HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLIT+NCNH
Sbjct: 62  HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITKNCNH 121

Query: 145 FCNDVCLKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKS 204
           FCND C+KL GKPIPSWVNRLARLGLFCNCVLPASLNE+KVRQVRSG+K NEG+KKKLK 
Sbjct: 122 FCNDACVKLVGKPIPSWVNRLARLGLFCNCVLPASLNESKVRQVRSGEKFNEGIKKKLKI 181

Query: 205 Q 206
           Q
Sbjct: 182 Q 182

[Close]

BLAST of Lsi04G020880 vs. ExPASy TrEMBL
Match: A0A6J1FKW1 (deSI-like protein At4g17486 OS=Cucurbita moschata OX=3662 GN=LOC111446669 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.1e-93
Identity = 162/181 (89.50%), Postives = 172/181 (95.03%), Query Frame = 0


Query: 25  IESYVLIAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHE 84
           +E    +A+MSKKKKSGTVPV+LNVYDLTPINGYAYW GLGVYHSGVQVHGVEYAFGAH+
Sbjct: 2   VEMLRRMALMSKKKKSGTVPVHLNVYDLTPINGYAYWFGLGVYHSGVQVHGVEYAFGAHD 61

Query: 85  HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNH 144
           HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLIT+NCNH
Sbjct: 62  HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITKNCNH 121

Query: 145 FCNDVCLKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKS 204
           FCND C+KL GKPIPSWVNRLARLGLFCNCVLPASLNE+KVRQVRSG+K NEG+KKKLK 
Sbjct: 122 FCNDACVKLVGKPIPSWVNRLARLGLFCNCVLPASLNESKVRQVRSGEKFNEGIKKKLKI 181

Query: 205 Q 206
           Q
Sbjct: 182 Q 182

[Close]

BLAST of Lsi04G020880 vs. NCBI nr
Match: XP_004145512.1 (deSI-like protein At4g17486 [Cucumis sativus] >KGN55449.1 hypothetical protein Csa_012914 [Cucumis sativus])

HSP 1 Score: 371.7 bits (953), Expect = 4.6e-99
Identity = 174/177 (98.31%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 5   IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 64

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 65  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 124

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPI 208
           ++LAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPI
Sbjct: 125 IRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQPI 181

[Close]

BLAST of Lsi04G020880 vs. NCBI nr
Match: XP_038896454.1 (deSI-like protein At4g17486 isoform X2 [Benincasa hispida])

HSP 1 Score: 368.2 bits (944), Expect = 5.1e-98
Identity = 172/176 (97.73%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 5   IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 64

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 65  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 124

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 207
           ++L GKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP
Sbjct: 125 VRLTGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 180

[Close]

BLAST of Lsi04G020880 vs. NCBI nr
Match: XP_038896452.1 (deSI-like protein At4g17486 isoform X1 [Benincasa hispida] >XP_038896453.1 deSI-like protein At4g17486 isoform X1 [Benincasa hispida])

HSP 1 Score: 368.2 bits (944), Expect = 5.1e-98
Identity = 172/176 (97.73%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 5   IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 64

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 65  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 124

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 207
           ++L GKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP
Sbjct: 125 VRLTGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 180

[Close]

BLAST of Lsi04G020880 vs. NCBI nr
Match: KAA0064599.1 (deSI-like protein [Cucumis melo var. makuwa] >TYK19991.1 deSI-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 367.5 bits (942), Expect = 8.7e-98
Identity = 171/176 (97.16%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 24  IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 83

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPK+VRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 84  FEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 143

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 207
           ++LAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKL+SQP
Sbjct: 144 IRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLRSQP 199

[Close]

BLAST of Lsi04G020880 vs. NCBI nr
Match: XP_008452873.1 (PREDICTED: deSI-like protein At4g17486 [Cucumis melo])

HSP 1 Score: 367.5 bits (942), Expect = 8.7e-98
Identity = 171/176 (97.16%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 31  IAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 90
           IA+MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI
Sbjct: 5   IALMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGI 64

Query: 91  FEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 150
           FEVEPRQCPGFTFRKSICIGRTNLGPK+VRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC
Sbjct: 65  FEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVC 124

Query: 151 LKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQP 207
           ++LAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKL+SQP
Sbjct: 125 IRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLRSQP 180

[Close]

BLAST of Lsi04G020880 vs. TAIR 10
Match: AT5G25170.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 297.0 bits (759), Expect = 1.3e-80
Identity = 135/177 (76.27%), Postives = 158/177 (89.27%), Query Frame = 0


Query: 30  LIAMMSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSG 89
           ++ +  +KKK G+VPVYLNVYDLTPINGYAYWLGLG+YHSGV+VHGVEY FGAH+H+T+G
Sbjct: 5   MVVVTGRKKKPGSVPVYLNVYDLTPINGYAYWLGLGIYHSGVEVHGVEYGFGAHDHSTTG 64

Query: 90  IFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDV 149
           IFEVEP+QCPGFTFRKSI IGRT+L P++VR FMEKLAEEYSGN+YHLIT+NCNHFCNDV
Sbjct: 65  IFEVEPKQCPGFTFRKSILIGRTDLDPENVRVFMEKLAEEYSGNSYHLITKNCNHFCNDV 124

Query: 150 CLKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRS-GDKLNEGVKKKLKSQ 206
           C++L  + IPSWVNRLAR GLFCNCVLPA LNE KVRQVRS  +K+ E  KKKL+S+
Sbjct: 125 CVQLTRRSIPSWVNRLARFGLFCNCVLPAELNETKVRQVRSKEEKIPEVEKKKLRSR 181

[Close]

BLAST of Lsi04G020880 vs. TAIR 10
Match: AT2G25190.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 278.1 bits (710), Expect = 6.5e-75
Identity = 127/172 (73.84%), Postives = 151/172 (87.79%), Query Frame = 0


Query: 34  MSKKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEV 93
           + +KK+SG+VPVYLNVYDLTP+N Y YWLGLGV+HSGV+VHGVEYAFGAHE +++GIFEV
Sbjct: 8   VKRKKQSGSVPVYLNVYDLTPMNAYGYWLGLGVFHSGVEVHGVEYAFGAHESSSTGIFEV 67

Query: 94  EPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKL 153
           EP++CPGFTFRKSI +G+T+L  K+VR FMEKLAEEY GN YHLITRNCNHFCN+VCLKL
Sbjct: 68  EPKKCPGFTFRKSILVGKTDLVAKEVRVFMEKLAEEYQGNKYHLITRNCNHFCNEVCLKL 127

Query: 154 AGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKLNEGVKKKLKSQ 206
           A K IP WVNRLARLG+ CNCVLP  LNEAKVR+V  G+ L+E  KKKL+++
Sbjct: 128 AQKSIPRWVNRLARLGVLCNCVLPPRLNEAKVRRVGKGE-LSESEKKKLRNR 178

[Close]

BLAST of Lsi04G020880 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 265.4 bits (677), Expect = 4.3e-71
Identity = 122/175 (69.71%), Postives = 141/175 (80.57%), Query Frame = 0


Query: 36  KKKKSGTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATSGIFEVEP 95
           +KK SGTVPVYLNVYDLTP+N Y YWLG+G+YHSG++VHGVEY +GAHE ++SGIFEVEP
Sbjct: 10  RKKNSGTVPVYLNVYDLTPMNVYGYWLGIGIYHSGLEVHGVEYGYGAHEKSSSGIFEVEP 69

Query: 96  RQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCNDVCLKLAG 155
           ++CPGFTFRKSI +G T +  K+VRSFMEKL+EEY GN YHLITRNCNHFCN V LKL  
Sbjct: 70  KKCPGFTFRKSILVGETEMKAKEVRSFMEKLSEEYQGNKYHLITRNCNHFCNHVSLKLTH 129

Query: 156 KPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGDKL---NEGVKKKLKSQPI 208
           K IPSWVNRLARLG  CNCVLPA LNE KV++V    KL    E  KKK + + I
Sbjct: 130 KSIPSWVNRLARLGFLCNCVLPACLNETKVKRVGKDGKLLLEGENTKKKKRKKKI 184

[Close]

BLAST of Lsi04G020880 vs. TAIR 10
Match: AT1G80690.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 250.4 bits (638), Expect = 1.4e-66
Identity = 117/181 (64.64%), Postives = 145/181 (80.11%), Query Frame = 0


Query: 33  MMSKKKKS----GTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEYAFGAHEHATS 92
           M+ +K  S    G VPVYLNVYDLTPINGYAYWLGLGVYHSGV+VHG+EYA+GAHE+ ++
Sbjct: 1   MLCRKNSSLVDRGNVPVYLNVYDLTPINGYAYWLGLGVYHSGVEVHGIEYAYGAHEYPST 60

Query: 93  GIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLITRNCNHFCND 152
           GIFE EP+QC GFTFRKSI IG+T+LGP +VR+ ME+LA+ Y G++Y+LIT+NCNHFC++
Sbjct: 61  GIFEGEPKQCEGFTFRKSILIGKTDLGPLEVRATMEQLADNYKGSSYNLITKNCNHFCDE 120

Query: 153 VCLKLAGKPIPSWVNRLARLGLFCNCVLPASLNEA-----KVRQVRSGDKLNEGVKKKLK 205
            C+KL G PIPSWVNRLAR+G  CNCVLPA++N       +V Q +S +  NE  KKKL 
Sbjct: 121 TCIKLTGNPIPSWVNRLARIGFMCNCVLPATINATRFGNNRVNQDKSCEAENE--KKKLT 179

[Close]

BLAST of Lsi04G020880 vs. TAIR 10
Match: AT1G47740.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 236.5 bits (602), Expect = 2.2e-62
Identity = 113/187 (60.43%), Postives = 131/187 (70.05%), Query Frame = 0


Query: 23  LRIESYVLIAMMSKKKKS----GTVPVYLNVYDLTPINGYAYWLGLGVYHSGVQVHGVEY 82
           L+ +S       SK K +    G  PVYLNVYDLTPINGY YW GLG++HSGV+VHGVEY
Sbjct: 45  LKSKSVARFCFFSKLKSNNHGPGRAPVYLNVYDLTPINGYIYWAGLGIFHSGVEVHGVEY 104

Query: 83  AFGAHEHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSGNTYHLI 142
           AFGAH++ATSG+FEVEPRQCPGF F+KSI IG TNL P  VR FME +A  Y GN YHLI
Sbjct: 105 AFGAHDYATSGVFEVEPRQCPGFKFKKSIFIGTTNLNPTQVREFMEDMACSYYGNMYHLI 164

Query: 143 TRNCNHFCNDVCLKLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGD-KLNEG 202
            +NCNHFC DVC KL GK IP WVNRLA++G  C+C+LP SL    V     G     E 
Sbjct: 165 VKNCNHFCQDVCYKLTGKKIPKWVNRLAQIGSVCSCILPESLKITAVCHDPDGQIPEEEN 224

Query: 203 VKKKLKS 205
            K+ L+S
Sbjct: 225 EKRSLRS 231

The following BLAST results are available for this feature:

BLAST of Lsi04G020880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VG8	2.9e-56	49.47	DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV...	[more]
H2KZK4	3.6e-30	40.24	Desumoylating isopeptidase 1 homolog OS=Caenorhabditis elegans OX=6239 GN=F36D4....	[more]
Q5PQ09	8.1e-30	45.59	Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1	[more]
Q6DC39	1.2e-28	44.12	Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1	[more]
Q5ZIV7	9.8e-28	42.96	Deubiquitinase DESI2 OS=Gallus gallus OX=9031 GN=DESI2 PE=2 SV=1	[more]

BLAST of Lsi04G020880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L339	2.2e-99	98.31	PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652130 PE=3 ...	[more]
A0A5A7VGG1	4.2e-98	97.16	DeSI-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00...	[more]
A0A1S3BVP4	4.2e-98	97.16	deSI-like protein At4g17486 OS=Cucumis melo OX=3656 GN=LOC103493768 PE=3 SV=1	[more]
A0A6J1ISX1	8.2e-94	90.06	deSI-like protein At4g17486 OS=Cucurbita maxima OX=3661 GN=LOC111480313 PE=3 SV=...	[more]
A0A6J1FKW1	3.1e-93	89.50	deSI-like protein At4g17486 OS=Cucurbita moschata OX=3662 GN=LOC111446669 PE=3 S...	[more]

BLAST of Lsi04G020880 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145512.1	4.6e-99	98.31	deSI-like protein At4g17486 [Cucumis sativus] >KGN55449.1 hypothetical protein C...	[more]
XP_038896454.1	5.1e-98	97.73	deSI-like protein At4g17486 isoform X2 [Benincasa hispida]	[more]
XP_038896452.1	5.1e-98	97.73	deSI-like protein At4g17486 isoform X1 [Benincasa hispida] >XP_038896453.1 deSI-...	[more]
KAA0064599.1	8.7e-98	97.16	deSI-like protein [Cucumis melo var. makuwa] >TYK19991.1 deSI-like protein [Cucu...	[more]
XP_008452873.1	8.7e-98	97.16	PREDICTED: deSI-like protein At4g17486 [Cucumis melo]	[more]

BLAST of Lsi04G020880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25170.1	1.3e-80	76.27	PPPDE putative thiol peptidase family protein	[more]
AT2G25190.1	6.5e-75	73.84	PPPDE putative thiol peptidase family protein	[more]
AT4G31980.1	4.3e-71	69.71	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,...	[more]
AT1G80690.1	1.4e-66	64.64	PPPDE putative thiol peptidase family protein	[more]
AT1G47740.1	2.2e-62	60.43	PPPDE putative thiol peptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008580	PPPDE peptidase domain	SMART	SM01179	DUF862_2a	coord: 43..181 e-value: 1.4E-57 score: 207.3
IPR008580	PPPDE peptidase domain	PFAM	PF05903	Peptidase_C97	coord: 44..178 e-value: 3.6E-49 score: 166.5
IPR008580	PPPDE peptidase domain	PANTHER	PTHR12378	DESUMOYLATING ISOPEPTIDASE	coord: 33..207
IPR008580	PPPDE peptidase domain	PROSITE	PS51858	PPPDE	coord: 43..180 score: 61.471375
IPR042266	PPPDE peptidase domain superfamily	GENE3D	3.90.1720.30	PPPDE domains	coord: 38..194 e-value: 8.0E-47 score: 160.8
None	No IPR available	PANTHER	PTHR12378:SF17	OS02G0788133 PROTEIN	coord: 33..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G020880.1	Lsi04G020880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
molecular_function	GO:0101005	deubiquitinase activity
molecular_function	GO:0008233	peptidase activity