Lsi04G020380 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G020380
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NADH-ubiquinone oxidoreductase chain 4
Location	chr04: 27487592 .. 27489613 (-)
RNA-Seq Expression	Lsi04G020380
Synteny	Lsi04G020380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGCCCATCTGTTTACTGTTGTCAACTAATCTCTTCAATGTTCAATTCGACTCAATGTTAGAACATTTCTGTGAATGCTATTCTGATTTAAGTGGTCCTATTCTGTGTCCCGTGCTAGGAAGCATTACTCCTCTTTTCATTCCAAATTCAAGAATACGACCGATACGATTGATTGGTCTATGTGCCTCTCTTATTACTTTTTTGTATCCCCCTGTTCCTCGGATACAATTCGATCCTTCTACGGCCAAATCTCAATTTGTGGAAAGCCTTCGATGGCTTCCTTATGAAAACATCAATTTTTATTTGGGTATAGACGGTATCTCTTTATTCTTCGTGATATTGACCACATTTCTGATCCCTATTTGCATTTTAGTGGGTTGGTCTGGTATGAGAAGTTATGGGAAAGAGTATATTACAGCATCTCTAATTCGTGAATTTCTAATGATCGCCGTGTTCCGCATGCTGGATCCTCTACTATTCTATGTTCTTCCCGAAAGCGTGCCAATCCCTATGTTGTGCGGAGCTGAGCATCTTCTATTCGCTGGGATCAAGCTTTTCCTCTGCAGGGGCCTTGTGCAGTAAACCCCCTACGGGCGGTCGTCCGTCGTCGTAAAGTAGTCAAATATTGCAAAGCCCCGGTATCTATTACCGGTCAGAATTGGACCATAACGGAATTTCGGTCTATACCGGCACCATAATATCTGATTGGGGAGGAAGATGAGAATTAGAGAAGTTATGTTTTCAAGTTTAAAGTGGATAATTTAGTTATCTTAATATAAATTATATAACAATTTTTTTAATACAACAATCGTAGAGATGGAGATCGAATTTTCGATCTCAATGTCAAGACAGGAAAGAAGAATGGGAGAATAACTAAACATGGGTGTGATAAGATGTAGAATTGAGAGGAAAACTTAGATATCTTAATAGTGATCTCACAAGATTTATAAAAGATGATTATTTAATTTAATTTTGAATTGGTTATGCTTCGATTAATTGTTCTTGAACGTGCTTAACAAAATTCTAAAATTGATCTCTTTCCTTAATTGAGTAAATTTGTTTAGGTATATAAGTACTATAACGTCAAAGGACTAAAGGTCTTTTCATTTGACTTATGCCACATCACACTTACAATGTATTTTCTTACTTATCTTGTTTTGTATGGACCATTCAATCTACCTTACTTTTTATGAAAGAGCATTTATCTGTATCTTTTAGGACAATATTAAGCTTAGTATGTTTATCCTACCTTTTTCTTTAGACGAATATTTATCTTGCCTTATTTTCTTAGGCTATTGAGCATCTATCCTCTAAGTCTTAGTATGTACTTACTTCACTTTCTCATACTATATATTTGATTTTATGGATGGTCTCGATTAATCCACGTGGACTTATCAACGGATGTCTTCGTAATTGTCCAAAGATTTGACTCATAGTCACAACATAATTATTCTTATCACATGTATATCTAAGAATTAATATACGATAAGACATATTAATAAGTAACACCCTAATTTACAACCAACATGAGTATAATTCAACGAACCAATATGAGTATAACTCAACGGTAATCAACATGTACTTTTTTTGTTTAAGGTCGAAGGTTTATATCACCATCTTCCACATTTTTCGAATCCAATATACAAATTTCAATCTTTTTTCAACCATATCATTCATAACCCATTTGTTTTTTTTTTTTTTTTTTTTTTTTTTAAATAAAAAAAAATAATGAACTGATAAATAATAATTCCTTTTTTGGGATGAAGAAAAGCAGTTGCCTGAGTCCAATCATAATAAATCTAATTCTGGAAGAGAAATGGGAAATGGAGACTGGAGAGGGACGTTTTGATGCTATTGATTGACTAAATTCACAATAACCCACCTTCAAGTTCTCAACAACACTCTCTCTCTTGCCGATCATACCCCATTTACTGTGAGTTCCTTCTCCCACTGCGCTCTTTTTTCCGTTTTAGCGATTTTCCTCTTTTGCTGCGCTTAATTACTGATTATCGTTATCTTTGA

mRNA sequence

GCGCCCATCTGTTTACTGTTGTCAACTAATCTCTTCAATGTTCAATTCGACTCAATGTTAGAACATTTCTGTGAATGCTATTCTGATTTAAGTGGTCCTATTCTGTGTCCCGTGCTAGGAAGCATTACTCCTCTTTTCATTCCAAATTCAAGAATACGACCGATACGATTGATTGGTCTATGTGCCTCTCTTATTACTTTTTTGTATCCCCCTGTTCCTCGGATACAATTCGATCCTTCTACGGCCAAATCTCAATTTGTGGAAAGCCTTCGATGGCTTCCTTATGAAAACATCAATTTTTATTTGGGTATAGACGGTATCTCTTTATTCTTCGTGATATTGACCACATTTCTGATCCCTATTTGCATTTTAGTGGGTTGGTCTGGTATGAGAAGTTATGGGAAAGAGTATATTACAGCATCTCTAATTCGTGAATTTCTAATGATCGCCGTGTTCCGCATGCTGGATCCTCTACTATTCTATGTTCTTCCCGAAAGCGTGCCAATCCCTATGTTGTGCGGAGCTGAGCATCTTCTATTCGCTGGGATCAAGCTTTTCCTCTGCAGGGGCCTTGTGCAGACAATATTAAGCTTAGCTATTGAGCATCTATCCTCTAAGTCTTACGATTTTCCTCTTTTGCTGCGCTTAATTACTGATTATCGTTATCTTTGA

Coding sequence (CDS)

GCGCCCATCTGTTTACTGTTGTCAACTAATCTCTTCAATGTTCAATTCGACTCAATGTTAGAACATTTCTGTGAATGCTATTCTGATTTAAGTGGTCCTATTCTGTGTCCCGTGCTAGGAAGCATTACTCCTCTTTTCATTCCAAATTCAAGAATACGACCGATACGATTGATTGGTCTATGTGCCTCTCTTATTACTTTTTTGTATCCCCCTGTTCCTCGGATACAATTCGATCCTTCTACGGCCAAATCTCAATTTGTGGAAAGCCTTCGATGGCTTCCTTATGAAAACATCAATTTTTATTTGGGTATAGACGGTATCTCTTTATTCTTCGTGATATTGACCACATTTCTGATCCCTATTTGCATTTTAGTGGGTTGGTCTGGTATGAGAAGTTATGGGAAAGAGTATATTACAGCATCTCTAATTCGTGAATTTCTAATGATCGCCGTGTTCCGCATGCTGGATCCTCTACTATTCTATGTTCTTCCCGAAAGCGTGCCAATCCCTATGTTGTGCGGAGCTGAGCATCTTCTATTCGCTGGGATCAAGCTTTTCCTCTGCAGGGGCCTTGTGCAGACAATATTAAGCTTAGCTATTGAGCATCTATCCTCTAAGTCTTACGATTTTCCTCTTTTGCTGCGCTTAATTACTGATTATCGTTATCTTTGA

Protein sequence

APICLLLSTNLFNVQFDSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQTILSLAIEHLSSKSYDFPLLLRLITDYRYL

Homology

[Close]

BLAST of Lsi04G020380 vs. ExPASy Swiss-Prot
Match: Q04050 (NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 4.9e-77
Identity = 155/208 (74.52%), Postives = 166/208 (79.81%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECYS+LSG ILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLY PVPRIQFD
Sbjct: 1   MLEHFCECYSNLSGLILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYSPVPRIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
            STAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICI VGWSGMRSYGKEYI
Sbjct: 61  SSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICISVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLC------GAEHLLFAGIKLFLCRGLV 198
           TA LIREFLMIAVFRMLD LLFYV PESVPIPM          +  + A  + FL   L 
Sbjct: 121 TAFLIREFLMIAVFRMLDLLLFYVFPESVPIPMFIIIGVWGSRQRKIKAAYQFFLYTLLG 180

Query: 199 QTILSLAIEHLSSKSYDFPLLLRLITDY 221
              + LAI  +  ++    L + L T++
Sbjct: 181 SLFMLLAILLILFQTGTTDLQISLTTEF 208

[Close]

BLAST of Lsi04G020380 vs. ExPASy Swiss-Prot
Match: P93313 (NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE=1 SV=2)

HSP 1 Score: 253.1 bits (645), Expect = 3.0e-66
Identity = 144/208 (69.23%), Postives = 155/208 (74.52%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECY +LSG ILCPVLGSI  LFIPNSRIR IRLIGLCASLITFLY  V  IQFD
Sbjct: 1   MLEHFCECYFNLSGLILCPVLGSIILLFIPNSRIRLIRLIGLCASLITFLYSLVLWIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
            STAK QFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI
Sbjct: 61  SSTAKFQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLC------GAEHLLFAGIKLFLCRGLV 198
            A LI EFLMIAVF MLD LLFYV PESV IPM          +  + A  + FL   L 
Sbjct: 121 IAFLICEFLMIAVFCMLDLLLFYVFPESVLIPMFIIIGVWGSRQRKIKAAYQFFLYTLLG 180

Query: 199 QTILSLAIEHLSSKSYDFPLLLRLITDY 221
              + LAI  +  ++    L + L T++
Sbjct: 181 SLFMLLAILLILFQTGTTDLQILLTTEF 208

[Close]

BLAST of Lsi04G020380 vs. ExPASy Swiss-Prot
Match: P27572 (NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-63
Identity = 138/204 (67.65%), Postives = 152/204 (74.51%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECY DLSG ILCPVLGSI  LFIPNS IR IRLIGLC SLITFLY  V  IQFD
Sbjct: 1   MLEHFCECYFDLSGLILCPVLGSIILLFIPNSSIRLIRLIGLCVSLITFLYSLVLWIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
           PSTAK QFVESLRWLPYENI+ Y+GIDG+SLFFVILTTFLIPICILVGWSGMRS+GKEYI
Sbjct: 61  PSTAKFQFVESLRWLPYENIHLYMGIDGLSLFFVILTTFLIPICILVGWSGMRSFGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLC------GAEHLLFAGIKLFL--CRG 198
            A LI EFLMIAVF MLD LLFYV  ESV IPM          +  + A  + FL    G
Sbjct: 121 IAFLICEFLMIAVFCMLDLLLFYVFFESVLIPMFIIIGVWGSRQRKIKAAYQFFLYTLLG 180

Query: 199 LVQTILSLAIEHLSSKSYDFPLLL 215
            V  +L++ +  L + + D  +LL
Sbjct: 181 SVFMLLAILLILLQTGTTDLQILL 204

[Close]

BLAST of Lsi04G020380 vs. ExPASy Swiss-Prot
Match: P26848 (NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 PE=3 SV=2)

HSP 1 Score: 172.2 bits (435), Expect = 6.7e-42
Identity = 105/208 (50.48%), Postives = 133/208 (63.94%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           ML+     YS+LSG IL P+LGS+  L IPNSR+R IR I +  SLITFLY     I+F+
Sbjct: 1   MLQVLAPFYSNLSGLILLPLLGSLIILVIPNSRVRLIRGITIWTSLITFLYSLFFWIRFE 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
             TAK QFVE++RWLPY NINFY+GIDGISLFFVILTTFL PICILVG+  ++SY KEY+
Sbjct: 61  NDTAKFQFVETIRWLPYSNINFYIGIDGISLFFVILTTFLTPICILVGFYSVKSYKKEYM 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLC------GAEHLLFAGIKLFLCRGLV 198
            A  I E  +IAVF  LD L+FYV  ESV IPM          +  + A  + FL   + 
Sbjct: 121 IAFFICESFLIAVFCSLDLLIFYVFFESVLIPMFIIIGVWGSRQRKIKAAYQFFLYTLMG 180

Query: 199 QTILSLAIEHLSSKSYDFPLLLRLITDY 221
              + LAI  +  ++    L + L T++
Sbjct: 181 SLFMLLAILFIFFQTGTTDLQILLTTEF 208

[Close]

BLAST of Lsi04G020380 vs. ExPASy Swiss-Prot
Match: Q37617 (NADH-ubiquinone oxidoreductase chain 4 OS=Prototheca wickerhamii OX=3111 GN=ND4 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.0e-26
Identity = 78/200 (39.00%), Postives = 112/200 (56.00%), Query Frame = 0


Query: 37  PVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFDPSTAKSQFVESL------ 96
           P+LG+I  LF+P+ + + IR I L +SL+TFL   +  I+FD S+A  QF + +      
Sbjct: 35  PLLGAIALLFVPSWKTQTIRNIALNSSLLTFLISLLLWIEFDSSSALFQFTDGVCSPNVY 94

Query: 97  ---------RWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYITAS 156
                        +  +NF LG+DGISLFF+ILTT L+PICILV W+ +  Y KEY  A 
Sbjct: 95  SDVTLAKAASSSSFSALNFALGVDGISLFFIILTTLLVPICILVSWNNIEVYVKEYCIAF 154

Query: 157 LIREFLMIAVFRMLDPLLFYVLPESVPIPMLC------GAEHLLFAGIKLFLCR--GLVQ 214
           L+ E LM+ VF +LD LLFY+  ESV IPM          E  + A  + FL    G V 
Sbjct: 155 LVLETLMLTVFSVLDLLLFYIFFESVLIPMFIIIGVWGSRERKIRAAYQFFLYTLFGSVL 214

[Close]

BLAST of Lsi04G020380 vs. ExPASy TrEMBL
Match: A0A5C7GR23 (Proton_antipo_M domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_027770 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.2e-97
Identity = 184/195 (94.36%), Postives = 185/195 (94.87%), Query Frame = 0


Query: 2   PICLLLSTNLFNVQFDSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLC 61
           PI LLLSTN FNV+F SMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLC
Sbjct: 449 PIFLLLSTNFFNVRFYSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLC 508

Query: 62  ASLITFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPI 121
           ASLITFLY PVPRIQFDPSTAKSQFVESLRWLPYENIN  LGIDGISLFFVILTTFLIPI
Sbjct: 509 ASLITFLYSPVPRIQFDPSTAKSQFVESLRWLPYENINLDLGIDGISLFFVILTTFLIPI 568

Query: 122 CILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFA 181
           CILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYV PESVPIPMLCGAEHLLFA
Sbjct: 569 CILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVFPESVPIPMLCGAEHLLFA 628

Query: 182 GIKLFLCRGLVQTIL 197
           GIKLFLCRGLV   L
Sbjct: 629 GIKLFLCRGLVHGYL 643

[Close]

BLAST of Lsi04G020380 vs. ExPASy TrEMBL
Match: A0A5C2FS99 (NADH dehydrogenase subunit 4 OS=Spondias mombin OX=80338 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.8e-93
Identity = 176/196 (89.80%), Postives = 183/196 (93.37%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLY PVPRIQFD
Sbjct: 1   MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYSPVPRIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
           PSTAKSQFVESLRWLPYENINF LGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI
Sbjct: 61  PSTAKSQFVESLRWLPYENINFDLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQTILSL 198
           TASLIREFLMIAVFRMLDPLLFYV PESVPIPMLCGAEHLLFAGIKLFLCRGLVQ +L++
Sbjct: 121 TASLIREFLMIAVFRMLDPLLFYVFPESVPIPMLCGAEHLLFAGIKLFLCRGLVQMLLAI 180

Query: 199 AIEHLSSKSYDFPLLL 215
            +  L + + D  + L
Sbjct: 181 LLILLQTGTTDLQISL 196

[Close]

BLAST of Lsi04G020380 vs. ExPASy TrEMBL
Match: A0A096XFA7 (Orf175 OS=Panax ginseng OX=4054 GN=orf175 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.3e-92
Identity = 172/175 (98.29%), Postives = 173/175 (98.86%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPV RIQFD
Sbjct: 1   MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVLRIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
           PSTAKSQFVESLRWLPYENI+FYLGIDGISLFFVILTTFLIPICI VGWSGMRSYGKEYI
Sbjct: 61  PSTAKSQFVESLRWLPYENIHFYLGIDGISLFFVILTTFLIPICISVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQ 194
           TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQ
Sbjct: 121 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQ 175

[Close]

BLAST of Lsi04G020380 vs. ExPASy TrEMBL
Match: A0A2I0IAQ2 (Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_038878 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 7.4e-92
Identity = 172/175 (98.29%), Postives = 173/175 (98.86%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECYSDLSG ILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYP VPRIQFD
Sbjct: 1   MLEHFCECYSDLSGLILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPLVPRIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
           PSTAKSQFVESL+WLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI
Sbjct: 61  PSTAKSQFVESLQWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQ 194
           TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQ
Sbjct: 121 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQ 175

[Close]

BLAST of Lsi04G020380 vs. ExPASy TrEMBL
Match: A0A6A4NCF0 (Putative NADH:ubiquinone reductase (H(+)-translocating) OS=Lupinus albus OX=3870 GN=Lalb_Chr00c24g0407401 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.7e-91
Identity = 170/175 (97.14%), Postives = 171/175 (97.71%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECYSDLSGPILCPVLGSI PLFIPNSRIRPIRLIGLCASLITFLY PVPRIQFD
Sbjct: 1   MLEHFCECYSDLSGPILCPVLGSIIPLFIPNSRIRPIRLIGLCASLITFLYSPVPRIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
           PSTAKSQFVESLRWLPYENINFYLGIDG+S FFVILTTFLIPICILVGWSGMRSYGKEYI
Sbjct: 61  PSTAKSQFVESLRWLPYENINFYLGIDGLSFFFVILTTFLIPICILVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQ 194
           TASLIREFLMIAVFRMLDPLLFYVLPESV IPMLCGAEHLLFAGIKLFLCRGLVQ
Sbjct: 121 TASLIREFLMIAVFRMLDPLLFYVLPESVLIPMLCGAEHLLFAGIKLFLCRGLVQ 175

[Close]

BLAST of Lsi04G020380 vs. NCBI nr
Match: KAG6621658.1 (hypothetical protein I3842_Q007500 [Carya illinoinensis])

HSP 1 Score: 370.5 bits (950), Expect = 9.9e-99
Identity = 183/188 (97.34%), Postives = 186/188 (98.94%), Query Frame = 0


Query: 6   LLSTNLFNVQFDSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLI 65
           +LSTNLFNV+F SMLEHFCECYSDLSG I+CPVLGSITPLFIPNSRIRPIRLIGLCASLI
Sbjct: 1   MLSTNLFNVRFYSMLEHFCECYSDLSGLIMCPVLGSITPLFIPNSRIRPIRLIGLCASLI 60

Query: 66  TFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILV 125
           TFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILV
Sbjct: 61  TFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILV 120

Query: 126 GWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKL 185
           GWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKL
Sbjct: 121 GWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKL 180

Query: 186 FLCRGLVQ 194
           FLCRGLVQ
Sbjct: 181 FLCRGLVQ 188

[Close]

BLAST of Lsi04G020380 vs. NCBI nr
Match: KAF9660741.1 (hypothetical protein SADUNF_SadunfMtG0004800 [Salix dunnii])

HSP 1 Score: 362.8 bits (930), Expect = 2.1e-96
Identity = 183/193 (94.82%), Postives = 186/193 (96.37%), Query Frame = 0


Query: 1   APICLLLSTNLFNVQFDSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGL 60
           API LLLSTNLFN++F SMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGL
Sbjct: 197 APIFLLLSTNLFNLRFYSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGL 256

Query: 61  CASLITFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIP 120
           CASLITFLY P PRIQFDPSTAKSQFVESLRWLPYENI+F LGIDGISLFFVILTTFLIP
Sbjct: 257 CASLITFLYSPAPRIQFDPSTAKSQFVESLRWLPYENIHFDLGIDGISLFFVILTTFLIP 316

Query: 121 ICILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLF 180
           ICILVGWSGMRSYGKEYITASLIREFLMIAVFRMLD LLFYVLPESV IPMLCGAEHLLF
Sbjct: 317 ICILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDLLLFYVLPESVLIPMLCGAEHLLF 376

Query: 181 AGIKLFLCRGLVQ 194
           AGIKLFLCRGLVQ
Sbjct: 377 AGIKLFLCRGLVQ 389

[Close]

BLAST of Lsi04G020380 vs. NCBI nr
Match: KAF3966570.1 (hypothetical protein CMV_009348 [Castanea mollissima])

HSP 1 Score: 362.5 bits (929), Expect = 2.7e-96
Identity = 181/188 (96.28%), Postives = 184/188 (97.87%), Query Frame = 0


Query: 6   LLSTNLFNVQFDSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLI 65
           +LSTNLFNV+F SMLEHFCECYSDLSG ILCPVLGSITPLFIPNSRIRPIRLIGLCASLI
Sbjct: 1   MLSTNLFNVRFYSMLEHFCECYSDLSGLILCPVLGSITPLFIPNSRIRPIRLIGLCASLI 60

Query: 66  TFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILV 125
           TFLYPPVPRIQFDPSTAKSQFVESLRWLPYENI+FYLGIDGISLFFVILTTFLIPICILV
Sbjct: 61  TFLYPPVPRIQFDPSTAKSQFVESLRWLPYENIHFYLGIDGISLFFVILTTFLIPICILV 120

Query: 126 GWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKL 185
           GWSGMRSYGKEYITASLIREFLMIAVF MLDPLLFYVLPESV IPMLCGAEHLLFAGIKL
Sbjct: 121 GWSGMRSYGKEYITASLIREFLMIAVFCMLDPLLFYVLPESVLIPMLCGAEHLLFAGIKL 180

Query: 186 FLCRGLVQ 194
           FLCRGLVQ
Sbjct: 181 FLCRGLVQ 188

[Close]

BLAST of Lsi04G020380 vs. NCBI nr
Match: KAG6736845.1 (hypothetical protein POTOM_060230 [Populus tomentosa])

HSP 1 Score: 359.4 bits (921), Expect = 2.3e-95
Identity = 181/191 (94.76%), Postives = 184/191 (96.34%), Query Frame = 0


Query: 1   APICLLLSTNLFNVQFDSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGL 60
           API LLLSTNLFN++F SMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGL
Sbjct: 229 APIFLLLSTNLFNLRFYSMLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGL 288

Query: 61  CASLITFLYPPVPRIQFDPSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIP 120
           CASLITFLY P PRIQFDPSTAKSQFVESLRWLPYENI+F LGIDGISLFFVILTTFLIP
Sbjct: 289 CASLITFLYSPAPRIQFDPSTAKSQFVESLRWLPYENIHFDLGIDGISLFFVILTTFLIP 348

Query: 121 ICILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLF 180
           ICILVGWSGMRSYGKEYITASLIREFLMIAVFRMLD LLFYVLPESV IPMLCGAEHLLF
Sbjct: 349 ICILVGWSGMRSYGKEYITASLIREFLMIAVFRMLDLLLFYVLPESVLIPMLCGAEHLLF 408

Query: 181 AGIKLFLCRGL 192
           AGIKLFLCRGL
Sbjct: 409 AGIKLFLCRGL 419

[Close]

BLAST of Lsi04G020380 vs. NCBI nr
Match: YP_009704587.1 (NADH dehydrogenase subunit 4 [Spondias mombin] >QEP09091.1 NADH dehydrogenase subunit 4 [Spondias mombin])

HSP 1 Score: 352.1 bits (902), Expect = 3.6e-93
Identity = 176/196 (89.80%), Postives = 183/196 (93.37%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLY PVPRIQFD
Sbjct: 1   MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYSPVPRIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
           PSTAKSQFVESLRWLPYENINF LGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI
Sbjct: 61  PSTAKSQFVESLRWLPYENINFDLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGIKLFLCRGLVQTILSL 198
           TASLIREFLMIAVFRMLDPLLFYV PESVPIPMLCGAEHLLFAGIKLFLCRGLVQ +L++
Sbjct: 121 TASLIREFLMIAVFRMLDPLLFYVFPESVPIPMLCGAEHLLFAGIKLFLCRGLVQMLLAI 180

Query: 199 AIEHLSSKSYDFPLLL 215
            +  L + + D  + L
Sbjct: 181 LLILLQTGTTDLQISL 196

[Close]

BLAST of Lsi04G020380 vs. TAIR 10
Match: ATMG00580.1 (NADH dehydrogenase subunit 4 )

HSP 1 Score: 285.0 bits (728), Expect = 5.1e-77
Identity = 154/208 (74.04%), Postives = 165/208 (79.33%), Query Frame = 0


Query: 19  MLEHFCECYSDLSGPILCPVLGSITPLFIPNSRIRPIRLIGLCASLITFLYPPVPRIQFD 78
           MLEHFCECYS+LSG ILCPVLGSIT LFIPNSRIRPIRLIGLCASLITFLY PVPRIQFD
Sbjct: 1   MLEHFCECYSNLSGLILCPVLGSITLLFIPNSRIRPIRLIGLCASLITFLYSPVPRIQFD 60

Query: 79  PSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICILVGWSGMRSYGKEYI 138
            STAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICI VGWSGMRSYGKEYI
Sbjct: 61  SSTAKSQFVESLRWLPYENINFYLGIDGISLFFVILTTFLIPICISVGWSGMRSYGKEYI 120

Query: 139 TASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLC------GAEHLLFAGIKLFLCRGLV 198
           TA LIREFLMIAVFRMLD LLFYV PESVPIPM          +  + A  + FL   L 
Sbjct: 121 TAFLIREFLMIAVFRMLDLLLFYVFPESVPIPMFIIIGVWGSRQRKIKAAYQFFLYTLLG 180

Query: 199 QTILSLAIEHLSSKSYDFPLLLRLITDY 221
              + LAI  +  ++    L + L T++
Sbjct: 181 SLFMLLAILLILFQTGTTDLQISLTTEF 208

The following BLAST results are available for this feature:

BLAST of Lsi04G020380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q04050	4.9e-77	74.52	NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=...	[more]
P93313	3.0e-66	69.23	NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE...	[more]
P27572	1.8e-63	67.65	NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 ...	[more]
P26848	6.7e-42	50.48	NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 P...	[more]
Q37617	4.0e-26	39.00	NADH-ubiquinone oxidoreductase chain 4 OS=Prototheca wickerhamii OX=3111 GN=ND4 ...	[more]

BLAST of Lsi04G020380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5C7GR23	1.2e-97	94.36	Proton_antipo_M domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62...	[more]
A0A5C2FS99	1.8e-93	89.80	NADH dehydrogenase subunit 4 OS=Spondias mombin OX=80338 PE=3 SV=1	[more]
A0A096XFA7	3.3e-92	98.29	Orf175 OS=Panax ginseng OX=4054 GN=orf175 PE=4 SV=1	[more]
A0A2I0IAQ2	7.4e-92	98.29	Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_038878 PE=4 SV=1	[more]
A0A6A4NCF0	3.7e-91	97.14	Putative NADH:ubiquinone reductase (H(+)-translocating) OS=Lupinus albus OX=3870...	[more]

BLAST of Lsi04G020380 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6621658.1	9.9e-99	97.34	hypothetical protein I3842_Q007500 [Carya illinoinensis]	[more]
KAF9660741.1	2.1e-96	94.82	hypothetical protein SADUNF_SadunfMtG0004800 [Salix dunnii]	[more]
KAF3966570.1	2.7e-96	96.28	hypothetical protein CMV_009348 [Castanea mollissima]	[more]
KAG6736845.1	2.3e-95	94.76	hypothetical protein POTOM_060230 [Populus tomentosa]	[more]
YP_009704587.1	3.6e-93	89.80	NADH dehydrogenase subunit 4 [Spondias mombin] >QEP09091.1 NADH dehydrogenase su...	[more]

BLAST of Lsi04G020380 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00580.1	5.1e-77	74.04	NADH dehydrogenase subunit 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR43507:SF15	PROTEIN, PUTATIVE-RELATED	coord: 28..196
IPR003918	NADH:ubiquinone oxidoreductase	PANTHER	PTHR43507	NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4	coord: 28..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G020380.1	Lsi04G020380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042773	ATP synthesis coupled electron transport
biological_process	GO:0015990	electron transport coupled proton transport
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0009579	thylakoid
molecular_function	GO:0008137	NADH dehydrogenase (ubiquinone) activity
molecular_function	GO:0048039	ubiquinone binding