Lsi04G020110 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G020110
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	polynucleotide 3'-phosphatase ZDP
Location	chr04: 27202963 .. 27207148 (+)
RNA-Seq Expression	Lsi04G020110
Synteny	Lsi04G020110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCATCTTCTGTAAAAATTGTCGCCGAGTATGCCAAGTCCAATCGCTCTACATGCAAGAATTGCTCCAAAACCATTCCTGCAAAAACACTCAGGTTGGGTTTTGTCACCAGAGATGGCCGGGGATTCGACATGACCCGCTGGTTTCATTCTGATTGCGCTTCTTTCGGTTCCGACCCTGTTTCTTCCGCCGAGGAGATTAATGGGTTCGCACTCTTGGAGGCATGTTTGAATACAAAACCTTAGTAGCGTCTGTTTTCTTTTCTCTGAAATGAGGACACGTTGACTGCTTTTGGTATTTTAGCGTAAAGGGGAAGTATGATTTGTGTTCATCCAGTGAAAGGGATGGTTTTTTTTTTACTGGGTCACTACGTGTTTGATAAAATGCTTCTAAGAGTCTATCATTTCATCTGGTTGGTTTTAGGAGCTTCTGTTTTCTTTTCTCTGAAATGGGGAGAAGTTGACTGCTTTTAGTAATAGTTTATGTTCGTCCAGTGAAAGGGATAGTTTTTTTACTGAGTCACCACACGTTTGATAAGATTCTTCTGAGATTTTATCCATTTCATCTGGTTGGTTTTGTTTGTTTTTCTTTTCAGGGTAGTGATCAGGAAGCTCTGAAGCAATTGGTTTGCAGAAGCCATGAATCTAAGGTGACTGTTTTGCTCACGTTTCTTTGTCATATTGCTTGCTTTATTATGCCCATCAGTCAGAATTTGGGTCTGGTTCTTTGGTTAAATTTTGTCCTTAATATATCTATTCTTCGCATTTTGGCTACTAAATGCATCTCCAATAAATAGGGCTGCAAAAGGGATGAGGATGAAGAAGATAGAGTTGAAGAAAGAGATCAAAAGAAAGTCAAGGTATGCATCCAGAAACCGACGCCCTTTGCAGAGCGTTTTCAGCATTTTCAAGTTATATTGGTCAATTCTATTGTTGATACAGTATGTTCATGTAATCAATGTAATTGTTGTACTGAAAGGGGCTCCTCCCTCTCTTTTATTTTACATACAATGTCTTTAGTCCTTGATGTACTGAACAATGTGAAATACTGCTATATAATTCTAGTTGTAGCAGCTTTTTGGAAATTCATATCCATTTGAAGTCATACTTTCTCTTCTCAATATCCATCTAAAACTAAAACAAATAGTTTGGTTAAGCTATTGAGTTGGAAAGTGCAATAGAATCCATTGAATGAAGCCTCCTCTTGTGCAATCGTTTGTTGCCTTATAATCTGGATCTTGAATTGGTTTTTAACTTGTTAACTTTGCTCAAATCTTCATTCTATATGATTTCATTGCTTATGCTGGCTGTGGTACTTGCTTTCTTAGTTTCTTGTTCAATTTCTCTAGATATTTATTCTGTGCTCTATGACACGTCTTATCTTCCGTATTTTGTACTTTCTTCTTCTTTAATGAATTTATACTTCTATTCTACATGTTGCACAATAAAATAGTAAAGTTAATAATACAGCATCCTCTTACTGTCATTTTATTGTTTGCAGTTGTCTACCTCTAGTTTGCAGCCAAAGTTAGATCTCGTCCTTACAGCTTCCAACATAAAGACCAAGTATAAGGTAAGATCCAGCCAATGTTATTTTCATTACTTGGTGCTTTTATCCTTTTTCCTTAACTGAAATGGATTTTGATTGTTGGCTGTATGAACTCACTTAAGCGTTGCTAGTTTAGGGGCAATACAAATTATAGAAATGTATATAATCCACGCCCAAAAGATGCATATAGGACAGATTCTATTCTAGACTAATCCATCCCCGCATTTTCTTTTAAGCTGTGGCATCAAAGATATTATTTCGCTTTCATATAATTCTATTCACAATTCATTCATAGCTTTGAATGATTCAGGATGCCACACTTTTACCTAAATGGAAGGCATTCCAGACGCTCATCTTTCTTGAGGAGGTAAAATCTTAATGATTTATGATTCTGTTTCTGAATCTTCGTGTTAATGTTGATTGCAATGTGGACAAATATGAATGAAAAGTTAGTAGAATGCTTATTTCTTCTGCTAAAACTCCATGTCTTTGAATTTAAAACTTGATTTGGACCATGTTAATTTTTAGTGCTTTAACACGTATTTAATGATTTATTGTAACAAATATTTGTCCAACTAAAACAATTAAACGCATAATTCAACTATTAGGTGTACACTTTTCAGATTATAACTCGGACAAATTGATATCGATGTACCCTTTACTATGTTGATGTGTTTCACTCAATTTGTTCATTTGCAGGATGATGGGCTACATCATTCAAGTAAAATAGCTGCATTTGATTTTGATGGCTGTTTGGCAAACACATCTGTTAAAAGGTATGGCATGTACCTGATGTGGAAATTTGGATTTTGCGTGCGTCCTTGTTTCATTCTTAAATAGCTAAAATGGTTTGGTTATAGGTTTTTCATAAACACTTTATTTCCATAATTACTCTTTACCTGCATTTGCTTTCACTAAATTCTCTCTCTATGCAGAGTTTTCTTCATGCATTTTCTTTTTTTATATTAATCTCTCTTCACATATTTCTCAATACGCACACATTTCTCTTTTTACACACACTTTCTTTAGCTTCTCTTCATATATCTTGAATCTCTTTCTTCATACCTTTAGTTTCTCTTGCTAATCTCTCTTCACTCGTCCAATTTCTCATACTAATCTGTCTCTATTCCCACTTTCTCCCACTTATTTCAGTTCTTGAAAAGTACAATTTTAATTGTATTCTAATTATAATGAGTCGTCAAGTCCAGAGAAACTCTATATTCAATGTTGGCAGTGCACTGAGAAATAAGAAGGGAAGGAAGGGTTTGTAACAATGGATGTATAGGTGTCCTGAATCCCCTTTTCTTATGGGGAAAAATTGAGTTTTTTTTTTTTTTTAATTATAATTATTATTGAAATTTTATCGGTACAAAGCAGTTTTGTTCTATGTTTAGACATGGGTTGGTTTAATTTCAGAGTAGGTGCAGAGGCGTGGTCTCTGATGTATCCTTCAATACCACAAAAATTTCAGAGCTTGTACGATGATGGCTACAAGCTGGTTTGTCACCCTTCCTCCATGAATCATATTTAACTATACTCTCTCTATCTTTGTTACGTAAATATTACTGATTAATATTACTATGAGCAGGTAATATTTACGAATGAATCCAACATTGAGAGATGGAAGAACAAGAGACAGGTTGCTGTGGACTCCAAACTTGGGCGTCTCAATAATTTTATCAGTCAAGTCAATGTCCCAGTTCAGGTAAATACCAAACCATGGTCACCACACACATTGCAAATGCTTCCATTCTTCACCCTGCAATATAACCATGTTTAATTGTGAGTGTATGATGTTGTTGTGTGGAATGTGGATGCAGGTGTTTGTTGCCTGTGGAATAGGAAGCGGATTGGGTAAATCCGAAGAAGACCCGTATAGGAAACCGAAACCTGGGATGTGGCATATCATGGAGAACCACTTCAATGGCGGCATCCCCGTTAATATAGACCAGTTATTTCTCTCTCCCTCTCTATCTTTCACTTATTATATTATCATTATCTTTCATGTGGAGGTATTTAGAGAAAGTAGAGATACTTCAACCATTTCAACCGATGAATTATGCTTATGTTGACAACTTTTTTCAACTGCCATTATATTGTATCATATTGTTAATGGCTTGCCCTGCCTTGGTTCATGTTGGTAGATGTTTCTATGTGGGGGATGCAGCTGGGAGAGCCAAGGATCACAGTGATGCTGATCTCAAGTTTGCTCAGGTACTACTAATTATTCTATTCACACTAATACTAACATATCCTAAAAAAAAATTGTGCATGGGTTTGACCATAATAGATGAATCAATTCTGTCAACATGTTTGTAATTTTATTTCAAGTTTAGTTTTCTGTGTATATATATATCTTCAGTTTGTTTTATTGCAATTATGCAGGCCATTGGGTTGAAGTTTTATGTTCCTGAAGAATTCTTTGTTGAGTAATTTATTTAACTTCTCTTTCACCGGCCTACCACTAGAAAAAGATTGTGCTTAGCAATGGCGTTGTAAATTGTAATTAATTACTTGAAGGGGAAGTTTTCTTCTCTGGCACTAGTATTAGTAGTTAGAACTTAGACTTGAAAACAGACCAATGCAGAAGCTGTAGTTGATTGTGATTCAATGGAGGATTTTCAATTCTGGCCC

mRNA sequence

ATGTCTCCATCTTCTGTAAAAATTGTCGCCGAGTATGCCAAGTCCAATCGCTCTACATGCAAGAATTGCTCCAAAACCATTCCTGCAAAAACACTCAGGTTGGGTTTTGTCACCAGAGATGGCCGGGGATTCGACATGACCCGCTGGTTTCATTCTGATTGCGCTTCTTTCGGTTCCGACCCTGTTTCTTCCGCCGAGGAGATTAATGGGTTCGCACTCTTGGAGGCATGTTTGAATACAAAACCTTATAATAGTTTATGTTCGTCCAGTGAAAGGGATAGTTTTTTTACTGAGTCACCACACGGTAGTGATCAGGAAGCTCTGAAGCAATTGGTTTGCAGAAGCCATGAATCTAAGGGCTGCAAAAGGGATGAGGATGAAGAAGATAGAGTTGAAGAAAGAGATCAAAAGAAAGTCAAGGTATGCATCCAGAAACCGACGCCCTTTGCAGAGCGTTTTCAGCATTTTCAAGTTATATTGGTCAATTCTATTGTTGATACATTGTCTACCTCTAGTTTGCAGCCAAAGTTAGATCTCGTCCTTACAGCTTCCAACATAAAGACCAAGTATAAGGATGCCACACTTTTACCTAAATGGAAGGCATTCCAGACGCTCATCTTTCTTGAGGAGGATGATGGGCTACATCATTCAAGTAAAATAGCTGCATTTGATTTTGATGGCTGTTTGGCAAACACATCTGTTAAAAGGTATGGCATAGTAGGTGCAGAGGCGTGGTCTCTGATGTATCCTTCAATACCACAAAAATTTCAGAGCTTGTACGATGATGGCTACAAGCTGGTAATATTTACGAATGAATCCAACATTGAGAGATGGAAGAACAAGAGACAGGTTGCTGTGGACTCCAAACTTGGGCGTCTCAATAATTTTATCAGTCAAGTCAATGTCCCAGTTCAGGTGTTTGTTGCCTGTGGAATAGGAAGCGGATTGGGTAAATCCGAAGAAGACCCGTATAGGAAACCGAAACCTGGGATGTGGCATATCATGGAGAACCACTTCAATGGCGGCATCCCCGTTAATATAGACCAATGTTTCTATGTGGGGGATGCAGCTGGGAGAGCCAAGGATCACAGTGATGCTGATCTCAAGTTTGCTCAGGCCATTGGGTTGAAGTTTTATGTTCCTGAAGAATTCTTTGTTGAGTAATTTATTTAACTTCTCTTTCACCGGCCTACCACTAGAAAAAGATTGTGCTTAGCAATGGCGTTGTAAATTGTAATTAATTACTTGAAGGGGAAGTTTTCTTCTCTGGCACTAGTATTAGTAGTTAGAACTTAGACTTGAAAACAGACCAATGCAGAAGCTGTAGTTGATTGTGATTCAATGGAGGATTTTCAATTCTGGCCC

Coding sequence (CDS)

ATGTCTCCATCTTCTGTAAAAATTGTCGCCGAGTATGCCAAGTCCAATCGCTCTACATGCAAGAATTGCTCCAAAACCATTCCTGCAAAAACACTCAGGTTGGGTTTTGTCACCAGAGATGGCCGGGGATTCGACATGACCCGCTGGTTTCATTCTGATTGCGCTTCTTTCGGTTCCGACCCTGTTTCTTCCGCCGAGGAGATTAATGGGTTCGCACTCTTGGAGGCATGTTTGAATACAAAACCTTATAATAGTTTATGTTCGTCCAGTGAAAGGGATAGTTTTTTTACTGAGTCACCACACGGTAGTGATCAGGAAGCTCTGAAGCAATTGGTTTGCAGAAGCCATGAATCTAAGGGCTGCAAAAGGGATGAGGATGAAGAAGATAGAGTTGAAGAAAGAGATCAAAAGAAAGTCAAGGTATGCATCCAGAAACCGACGCCCTTTGCAGAGCGTTTTCAGCATTTTCAAGTTATATTGGTCAATTCTATTGTTGATACATTGTCTACCTCTAGTTTGCAGCCAAAGTTAGATCTCGTCCTTACAGCTTCCAACATAAAGACCAAGTATAAGGATGCCACACTTTTACCTAAATGGAAGGCATTCCAGACGCTCATCTTTCTTGAGGAGGATGATGGGCTACATCATTCAAGTAAAATAGCTGCATTTGATTTTGATGGCTGTTTGGCAAACACATCTGTTAAAAGGTATGGCATAGTAGGTGCAGAGGCGTGGTCTCTGATGTATCCTTCAATACCACAAAAATTTCAGAGCTTGTACGATGATGGCTACAAGCTGGTAATATTTACGAATGAATCCAACATTGAGAGATGGAAGAACAAGAGACAGGTTGCTGTGGACTCCAAACTTGGGCGTCTCAATAATTTTATCAGTCAAGTCAATGTCCCAGTTCAGGTGTTTGTTGCCTGTGGAATAGGAAGCGGATTGGGTAAATCCGAAGAAGACCCGTATAGGAAACCGAAACCTGGGATGTGGCATATCATGGAGAACCACTTCAATGGCGGCATCCCCGTTAATATAGACCAATGTTTCTATGTGGGGGATGCAGCTGGGAGAGCCAAGGATCACAGTGATGCTGATCTCAAGTTTGCTCAGGCCATTGGGTTGAAGTTTTATGTTCCTGAAGAATTCTTTGTTGAGTAA

Protein sequence

MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSDPVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKGCKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDLVLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQVNVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGRAKDHSDADLKFAQAIGLKFYVPEEFFVE

Homology

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BLAST of Lsi04G020110 vs. ExPASy Swiss-Prot
Match: Q84JE8 (Polynucleotide 3'-phosphatase ZDP OS=Arabidopsis thaliana OX=3702 GN=ZDP PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.4e-111
Identity = 211/396 (53.28%), Postives = 273/396 (68.94%), Query Frame = 0


Query: 2   SPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSDP 61
           SP S K+++EYAKS+RSTCK CS+TI AK LRLG VTR+ RGFDM +W H  C    SDP
Sbjct: 322 SPDSSKVISEYAKSSRSTCKKCSQTIAAKELRLGLVTRNFRGFDMKQWHHLGCFPVDSDP 381

Query: 62  VSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCR-SHESKG 121
           + S E+I GF+ L++                           DQ+ALK+LV +   ++  
Sbjct: 382 IVSVEDIGGFSELQS--------------------------GDQDALKELVQQCGKQTLV 441

Query: 122 CKRDEDEED---------RVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTS 181
            K DED +D            +R   +V   +++     +  Q  Q+   + +  + STS
Sbjct: 442 DKMDEDNDDTEAKIKLTEETNKRKHSEVGEMVEEDESLTKAKQ--QMAKTHKVNMSESTS 501

Query: 182 SLQPKLDLVLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLAN 241
            ++ + ++ L+AS++K KY+DA LLPKWKAF+T+IFLE DDGL+ S KIAAFDFDGCLA 
Sbjct: 502 QVEVEAEITLSASDVKDKYRDANLLPKWKAFETVIFLERDDGLNDSEKIAAFDFDGCLAK 561

Query: 242 TSVKRYGIVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLG 301
           TSVK   IVGA+AWSLMYPSIP+K QSL+D GYKLVIFTNESNI+RWKNKRQ AVDSK+G
Sbjct: 562 TSVK---IVGADAWSLMYPSIPEKLQSLHDQGYKLVIFTNESNIDRWKNKRQAAVDSKIG 621

Query: 302 RLNNFISQVNVPVQVFVACGIGSGLGK-SEEDPYRKPKPGMWHIMENHFNGGIPVNIDQC 361
           RLN+FI +V VP+QVF+ACG+ S  GK  ++D YRKPK GMW +M+ HFN GI +++D+ 
Sbjct: 622 RLNSFIERVKVPIQVFIACGVSSSGGKGGKDDLYRKPKAGMWQLMKKHFNSGIAIDMDKS 681

Query: 362 FYVGDAAGRAKDHSDADLKFAQAIGLKFYVPEEFFV 387
           FYVGDAAGR  DHSDAD+KFAQA GLKF+ PEE+F+
Sbjct: 682 FYVGDAAGRKMDHSDADIKFAQASGLKFFTPEEYFI 686

[Close]

BLAST of Lsi04G020110 vs. ExPASy Swiss-Prot
Match: Q9JLV6 (Bifunctional polynucleotide phosphatase/kinase OS=Mus musculus OX=10090 GN=Pnkp PE=1 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 2.1e-27
Identity = 73/198 (36.87%), Postives = 101/198 (51.01%), Query Frame = 0


Query: 199 WKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIVGAEAWSLMYPSIPQKFQS 258
           W++ + L+      G+    K+AAFD DG L  T   +        W ++YP IP+K Q 
Sbjct: 146 WESLKKLLVFTA-SGVKPQGKVAAFDLDGTLITTRSGKVFPTSPSDWRILYPEIPKKLQE 205

Query: 259 LYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQVNVPVQVFVACGIGSGLGK 318
           L  +GYKLVIFTN+  I R K   +V      G++   + ++ VP QV VA   G     
Sbjct: 206 LAAEGYKLVIFTNQMGIGRGKLPAEVF----KGKVEAVLEKLGVPFQVLVATHAGLN--- 265

Query: 319 SEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGR---------AKDHSDADLK 378
                 RKP  GMW  ++   N GIP++++   +VGDAAGR          KD S AD  
Sbjct: 266 ------RKPVSGMWDHLQEQANEGIPISVEDSVFVGDAAGRLANWAPGRKKKDFSCADRL 325

Query: 379 FAQAIGLKFYVPEEFFVE 388
           FA  +GL F  PEEFF++
Sbjct: 326 FALNVGLPFATPEEFFLK 329

[Close]

BLAST of Lsi04G020110 vs. ExPASy Swiss-Prot
Match: Q96T60 (Bifunctional polynucleotide phosphatase/kinase OS=Homo sapiens OX=9606 GN=PNKP PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.8e-27
Identity = 72/200 (36.00%), Postives = 100/200 (50.00%), Query Frame = 0


Query: 197 PKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIVGAEAWSLMYPSIPQKF 256
           P W+  + L+      G+    K+A FD DG L  T   +    G   W ++YP IP+K 
Sbjct: 145 PGWENLEKLLVFTA-AGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKL 204

Query: 257 QSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQVNVPVQVFVACGIGSGL 316
           + L  +GYKLVIFTN+ +I R K    +  +    ++   + ++ VP QV VA   G   
Sbjct: 205 RELEAEGYKLVIFTNQMSIGRGK----LPAEEFKAKVEAVVEKLGVPFQVLVATHAGL-- 264

Query: 317 GKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGR---------AKDHSDAD 376
                  YRKP  GMW  ++   N G P++I    +VGDAAGR          KD S AD
Sbjct: 265 -------YRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCAD 324

Query: 377 LKFAQAIGLKFYVPEEFFVE 388
             FA  +GL F  PEEFF++
Sbjct: 325 RLFALNLGLPFATPEEFFLK 330

[Close]

BLAST of Lsi04G020110 vs. ExPASy Swiss-Prot
Match: Q19683 (Uncharacterized protein F21D5.5 OS=Caenorhabditis elegans OX=6239 GN=F21D5.5 PE=2 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 1.0e-24
Identity = 67/174 (38.51%), Postives = 92/174 (52.87%), Query Frame = 0


Query: 219 KIAAFDFDGCLANTSVKRYGIVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERW 278
           KIAAFD DG L  T   +      + W L+Y SIP  F+ L+ DG+K+VIFTN+  I   
Sbjct: 52  KIAAFDMDGTLIKTKSGKVFPTNCQDWQLLYDSIPSDFKKLHSDGFKIVIFTNQKGIHAG 111

Query: 279 KNKRQVAVDSKLGRLNNFISQVNVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENH 338
           K  R    +    ++   + ++ +PVQ FV+   G          YRKP  GMW+ ++  
Sbjct: 112 KVDR----NEFRKKIEAIVGKLGIPVQAFVSVAGGH---------YRKPCVGMWNELKLR 171

Query: 339 FNGGIPVNIDQCFYVGDAAGR-------AKDHSDADLKFAQAIGLKFYVPEEFF 386
            N  + +N  +  +VGDAAGR        KDHS AD  FA  +G+KF  PEEFF
Sbjct: 172 -NDEVEINEKESIFVGDAAGRIKTTSRPKKDHSYADRFFAANVGVKFQTPEEFF 211

[Close]

BLAST of Lsi04G020110 vs. ExPASy Swiss-Prot
Match: O13911 (Bifunctional polynucleotide phosphatase/kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pnk1 PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 1.2e-22
Identity = 64/183 (34.97%), Postives = 96/183 (52.46%), Query Frame = 0


Query: 206 IFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIVGAEAWSLMYPSIPQKFQSLYDDGYK 265
           +++ +   L  + K  AFD DG L  T   R     A  W+  +PS+  K ++LY D Y 
Sbjct: 56  LYIAKYGKLKKTKKFIAFDLDGTLIKTKSGRVFSKDAADWTWWHPSVVPKLKALYQDNYS 115

Query: 266 LVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQVNVPVQVFVACGIGSGLGKSEEDPYR 325
           LVIF+N++ I R    +  A  +   ++      +++P+ ++ A           +D +R
Sbjct: 116 LVIFSNQNGIPR----KPSAGHTFQMKIRAIFESLDLPIVLYAAI---------LKDKFR 175

Query: 326 KPKPGMWHIMENHFNGGIPVNIDQCF--YVGDAAGRAKDHSDADLKFAQAIGLKFYVPEE 385
           KP  GMW    N F   +  +ID  F  YVGDAAGR  DH+  DLKFA+ IG+KF  PE+
Sbjct: 176 KPLTGMW----NSFLKDVNRSIDLSFIKYVGDAAGRPGDHNSTDLKFAENIGIKFETPEQ 221

Query: 386 FFV 387
           FF+
Sbjct: 236 FFL 221

[Close]

BLAST of Lsi04G020110 vs. ExPASy TrEMBL
Match: A0A0A0KZI4 (PARP-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G627160 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.4e-164
Identity = 308/390 (78.97%), Postives = 323/390 (82.82%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS SS+KIVAEYAKSNRSTCKNCSKTIPA TLRLGFVTRDGRGFDMTRWFHSDCASFG  
Sbjct: 39  MSSSSLKIVAEYAKSNRSTCKNCSKTIPASTLRLGFVTRDGRGFDMTRWFHSDCASFGPR 98

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAE+INGFALL+                           SDQEAL+++VCRSHESK 
Sbjct: 99  PVSSAEDINGFALLK--------------------------DSDQEALRKMVCRSHESKV 158

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQ---KPTPFAERFQHFQVILVNSIVDTLSTSSLQPKL 180
            KRDEDEED VEE DQKKVKV  +   +     ER Q             LSTSSLQPKL
Sbjct: 159 HKRDEDEEDGVEEGDQKKVKVHKKDGVEEDGVEERDQKNV---------KLSTSSLQPKL 218

Query: 181 DLVLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRY 240
           D+VLTASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR 
Sbjct: 219 DIVLTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR- 278

Query: 241 GIVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFI 300
             VGAEAWSLMYPSIPQK QSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFI
Sbjct: 279 --VGAEAWSLMYPSIPQKLQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFI 338

Query: 301 SQVNVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAA 360
           SQV VPVQVFVACGIGS  GKSEED +RKPKPGMWHIME+HFNGGIP++IDQCFYVGDAA
Sbjct: 339 SQVKVPVQVFVACGIGSKSGKSEEDMFRKPKPGMWHIMESHFNGGIPIDIDQCFYVGDAA 390

Query: 361 GRAKDHSDADLKFAQAIGLKFYVPEEFFVE 388
           GRAKDHSDAD+KFAQAIGLKFYVPEEFFVE
Sbjct: 399 GRAKDHSDADIKFAQAIGLKFYVPEEFFVE 390

[Close]

BLAST of Lsi04G020110 vs. ExPASy TrEMBL
Match: A0A1S3CLI9 (polynucleotide 3'-phosphatase ZDP OS=Cucumis melo OX=3656 GN=LOC103501815 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 6.9e-162
Identity = 305/387 (78.81%), Postives = 324/387 (83.72%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS SS+KIVAEYAKSNRSTCKNCSKTIPA TLRLGFVTRDGRGFDMTRWFHS CASFGS 
Sbjct: 40  MSSSSLKIVAEYAKSNRSTCKNCSKTIPASTLRLGFVTRDGRGFDMTRWFHSHCASFGSH 99

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAE+INGFALL+                           SDQEALK++VCRSHESK 
Sbjct: 100 PVSSAEDINGFALLK--------------------------DSDQEALKKMVCRSHESKV 159

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDLV 180
            KRD DE+D VEERDQKKVKV  QK     +  + + V   +     LSTSSLQPKLDLV
Sbjct: 160 HKRDGDEKDGVEERDQKKVKV-HQK-----DGDEEYGVEERDQKNVKLSTSSLQPKLDLV 219

Query: 181 LTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIV 240
           LTAS+IKTKYKDATLL KWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR   V
Sbjct: 220 LTASDIKTKYKDATLLAKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR---V 279

Query: 241 GAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 300
           GAEAWSLMYPSIPQK QSL+DDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV
Sbjct: 280 GAEAWSLMYPSIPQKLQSLHDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 339

Query: 301 NVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGRA 360
            VPVQVFVACGIGS  GKSEED +RKPKPGMWHIME+HFNGG+P++IDQCFYVGDAAGRA
Sbjct: 340 KVPVQVFVACGIGSKSGKSEEDTFRKPKPGMWHIMESHFNGGVPIDIDQCFYVGDAAGRA 391

Query: 361 KDHSDADLKFAQAIGLKFYVPEEFFVE 388
           KDHSDAD+KFAQAIGLKFYVPEEFFVE
Sbjct: 400 KDHSDADIKFAQAIGLKFYVPEEFFVE 391

[Close]

BLAST of Lsi04G020110 vs. ExPASy TrEMBL
Match: A0A6J1IH48 (polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476896 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 299/387 (77.26%), Postives = 317/387 (81.91%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS S+VKIVAEYAKSNRSTCKNCSKTIPAK LRLGFV+RDGRGFDMTRWFHSDCASFGSD
Sbjct: 40  MSTSAVKIVAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSD 99

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAE+INGFALL+                          G DQEALK+LV RSHESK 
Sbjct: 100 PVSSAEQINGFALLK--------------------------GDDQEALKKLVSRSHESKV 159

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDLV 180
            KRDEDEED +EERDQKKV+                           LS SSL PKLDLV
Sbjct: 160 RKRDEDEEDGIEERDQKKVE---------------------------LSASSLLPKLDLV 219

Query: 181 LTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIV 240
           L+ASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR   V
Sbjct: 220 LSASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR---V 279

Query: 241 GAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 300
           GAEAWSLMYPSIPQK QSLY++GYKLVIFTNESNIERWKNKRQVAVDSKLGRL++FIS+V
Sbjct: 280 GAEAWSLMYPSIPQKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRV 339

Query: 301 NVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGRA 360
           NVPVQVFVACGIGSG GKSEEDP+RKPKPGMW IMENHFNGGIP+N+DQCFYVGDAAGR 
Sbjct: 340 NVPVQVFVACGIGSGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRG 370

Query: 361 KDHSDADLKFAQAIGLKFYVPEEFFVE 388
           KDHSDADL+FAQAIGLKFYVPEE FVE
Sbjct: 400 KDHSDADLRFAQAIGLKFYVPEEIFVE 370

[Close]

BLAST of Lsi04G020110 vs. ExPASy TrEMBL
Match: A0A6J1IFZ5 (polynucleotide 3'-phosphatase ZDP isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476896 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 299/387 (77.26%), Postives = 317/387 (81.91%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS S+VKIVAEYAKSNRSTCKNCSKTIPAK LRLGFV+RDGRGFDMTRWFHSDCASFGSD
Sbjct: 7   MSTSAVKIVAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSD 66

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAE+INGFALL+                          G DQEALK+LV RSHESK 
Sbjct: 67  PVSSAEQINGFALLK--------------------------GDDQEALKKLVSRSHESKV 126

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDLV 180
            KRDEDEED +EERDQKKV+                           LS SSL PKLDLV
Sbjct: 127 RKRDEDEEDGIEERDQKKVE---------------------------LSASSLLPKLDLV 186

Query: 181 LTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIV 240
           L+ASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR   V
Sbjct: 187 LSASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR---V 246

Query: 241 GAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 300
           GAEAWSLMYPSIPQK QSLY++GYKLVIFTNESNIERWKNKRQVAVDSKLGRL++FIS+V
Sbjct: 247 GAEAWSLMYPSIPQKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRV 306

Query: 301 NVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGRA 360
           NVPVQVFVACGIGSG GKSEEDP+RKPKPGMW IMENHFNGGIP+N+DQCFYVGDAAGR 
Sbjct: 307 NVPVQVFVACGIGSGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRG 337

Query: 361 KDHSDADLKFAQAIGLKFYVPEEFFVE 388
           KDHSDADL+FAQAIGLKFYVPEE FVE
Sbjct: 367 KDHSDADLRFAQAIGLKFYVPEEIFVE 337

[Close]

BLAST of Lsi04G020110 vs. ExPASy TrEMBL
Match: A0A6J1F9Q9 (polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443571 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.6e-161
Identity = 299/387 (77.26%), Postives = 315/387 (81.40%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS S+VKIVAEYAKSNRSTCKNCSKTIPAK LRLGFV+RDGRGFDMTRWFHSDCASFGSD
Sbjct: 40  MSTSAVKIVAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSD 99

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAEEINGFALL+                          G DQEALK+LV RSHESK 
Sbjct: 100 PVSSAEEINGFALLK--------------------------GDDQEALKKLVSRSHESKV 159

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDLV 180
            KRDEDEED +EERDQKKV+                           LS SSL PKLDLV
Sbjct: 160 RKRDEDEEDGIEERDQKKVE---------------------------LSASSLLPKLDLV 219

Query: 181 LTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIV 240
           LTASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR   V
Sbjct: 220 LTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR---V 279

Query: 241 GAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 300
           GAEAWSLMYP IP K QSLY++GYKLVIFTNESNIERWKNKRQVAVDSKLGRL++FIS+V
Sbjct: 280 GAEAWSLMYPLIPHKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRV 339

Query: 301 NVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGRA 360
           NVPVQVFVACGIG G GKSEEDP+RKPKPGMW IMENHFNGGIP+N+DQCFYVGDAAGRA
Sbjct: 340 NVPVQVFVACGIGGGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRA 370

Query: 361 KDHSDADLKFAQAIGLKFYVPEEFFVE 388
           KDHSD DL+FAQAIGLKFYVPEEFFVE
Sbjct: 400 KDHSDTDLRFAQAIGLKFYVPEEFFVE 370

[Close]

BLAST of Lsi04G020110 vs. NCBI nr
Match: XP_004146024.2 (polynucleotide 3'-phosphatase ZDP isoform X1 [Cucumis sativus] >KGN55065.1 hypothetical protein Csa_012751 [Cucumis sativus])

HSP 1 Score: 586.6 bits (1511), Expect = 1.5e-163
Identity = 308/390 (78.97%), Postives = 323/390 (82.82%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS SS+KIVAEYAKSNRSTCKNCSKTIPA TLRLGFVTRDGRGFDMTRWFHSDCASFG  
Sbjct: 39  MSSSSLKIVAEYAKSNRSTCKNCSKTIPASTLRLGFVTRDGRGFDMTRWFHSDCASFGPR 98

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAE+INGFALL+                           SDQEAL+++VCRSHESK 
Sbjct: 99  PVSSAEDINGFALLK--------------------------DSDQEALRKMVCRSHESKV 158

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQ---KPTPFAERFQHFQVILVNSIVDTLSTSSLQPKL 180
            KRDEDEED VEE DQKKVKV  +   +     ER Q             LSTSSLQPKL
Sbjct: 159 HKRDEDEEDGVEEGDQKKVKVHKKDGVEEDGVEERDQKNV---------KLSTSSLQPKL 218

Query: 181 DLVLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRY 240
           D+VLTASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR 
Sbjct: 219 DIVLTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR- 278

Query: 241 GIVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFI 300
             VGAEAWSLMYPSIPQK QSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFI
Sbjct: 279 --VGAEAWSLMYPSIPQKLQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFI 338

Query: 301 SQVNVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAA 360
           SQV VPVQVFVACGIGS  GKSEED +RKPKPGMWHIME+HFNGGIP++IDQCFYVGDAA
Sbjct: 339 SQVKVPVQVFVACGIGSKSGKSEEDMFRKPKPGMWHIMESHFNGGIPIDIDQCFYVGDAA 390

Query: 361 GRAKDHSDADLKFAQAIGLKFYVPEEFFVE 388
           GRAKDHSDAD+KFAQAIGLKFYVPEEFFVE
Sbjct: 399 GRAKDHSDADIKFAQAIGLKFYVPEEFFVE 390

[Close]

BLAST of Lsi04G020110 vs. NCBI nr
Match: KAG6591920.1 (Polynucleotide 3'-phosphatase ZDP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 585.5 bits (1508), Expect = 3.4e-163
Identity = 303/387 (78.29%), Postives = 319/387 (82.43%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS S+VKIVAEYAKSNRSTCKNCSKTIPAK LRLGFV+RDGRGFDMTRWFHSDCASFGSD
Sbjct: 1   MSTSAVKIVAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSD 60

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAEEINGFALL+                          G DQEALK+LV RSHESK 
Sbjct: 61  PVSSAEEINGFALLK--------------------------GDDQEALKKLVSRSHESKV 120

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDLV 180
            KRDEDEED +EERDQKKV+                           LS SSL PKLDLV
Sbjct: 121 RKRDEDEEDGIEERDQKKVE---------------------------LSASSLLPKLDLV 180

Query: 181 LTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIV 240
           LTASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR   V
Sbjct: 181 LTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR---V 240

Query: 241 GAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 300
           GAEAWSLMYPSIPQK QSLY++GYKLVIFTNESNIERWKNKRQVAVDSKLGRL++FIS+V
Sbjct: 241 GAEAWSLMYPSIPQKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFISRV 300

Query: 301 NVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGRA 360
           NVPVQVFVACGIGSG GKSEEDP+RKPKPGMW IMENHFNGGIP+N+DQCFYVGDAAGRA
Sbjct: 301 NVPVQVFVACGIGSGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAGRA 331

Query: 361 KDHSDADLKFAQAIGLKFYVPEEFFVE 388
           KDHSDADL+FAQAIGLKFYVPEEFFVE
Sbjct: 361 KDHSDADLRFAQAIGLKFYVPEEFFVE 331

[Close]

BLAST of Lsi04G020110 vs. NCBI nr
Match: XP_038898753.1 (polynucleotide 3'-phosphatase ZDP [Benincasa hispida])

HSP 1 Score: 582.8 bits (1501), Expect = 2.2e-162
Identity = 306/388 (78.87%), Postives = 315/388 (81.19%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMT-RWFHSDCASFGS 60
           MS SS KIVAEYAKSNRSTCKNCSKTIPA TLRLGFV RDGRGFDMT RWFHSDCASFGS
Sbjct: 51  MSSSSPKIVAEYAKSNRSTCKNCSKTIPANTLRLGFVIRDGRGFDMTPRWFHSDCASFGS 110

Query: 61  DPVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESK 120
           DPVSSAE+INGFALL+                            DQEALK+LVCRSHESK
Sbjct: 111 DPVSSAEDINGFALLK--------------------------DIDQEALKKLVCRSHESK 170

Query: 121 GCKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDL 180
             K D DEED VEERDQKKVK                           LSTSSLQPKL+L
Sbjct: 171 VYKTDRDEEDGVEERDQKKVK---------------------------LSTSSLQPKLNL 230

Query: 181 VLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGI 240
           VLTASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR   
Sbjct: 231 VLTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR--- 290

Query: 241 VGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQ 300
           VGAEAWSLMYPSIPQK +SLY+DGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQ
Sbjct: 291 VGAEAWSLMYPSIPQKLRSLYNDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQ 350

Query: 301 VNVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGR 360
           VNVPVQVFVACGIGSG GKSEEDP+RKPKPGMWH+ME HFN GIPVNIDQCFYVGDAAGR
Sbjct: 351 VNVPVQVFVACGIGSGSGKSEEDPFRKPKPGMWHVMEEHFNDGIPVNIDQCFYVGDAAGR 382

Query: 361 AKDHSDADLKFAQAIGLKFYVPEEFFVE 388
           AKDHSD DLKFAQAIGLKFYVPEEFFVE
Sbjct: 411 AKDHSDTDLKFAQAIGLKFYVPEEFFVE 382

[Close]

BLAST of Lsi04G020110 vs. NCBI nr
Match: KAG7024794.1 (Polynucleotide 3'-phosphatase ZDP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 581.6 bits (1498), Expect = 4.9e-162
Identity = 306/389 (78.66%), Postives = 322/389 (82.78%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS S+VKIVAEYAKSNRSTCKNCSKTIPAK LRLGFV+RDGRGFDMTRWFHSDCASFGSD
Sbjct: 40  MSTSAVKIVAEYAKSNRSTCKNCSKTIPAKALRLGFVSRDGRGFDMTRWFHSDCASFGSD 99

Query: 61  PVSSAEEINGFALL--EACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHES 120
           PVSSAEEING   L  +  L  K    LCS       F     G DQEALK+LV RSHES
Sbjct: 100 PVSSAEEINGVHYLFDKMLLRFK----LCS-------FHLLGFGDDQEALKKLVSRSHES 159

Query: 121 KGCKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLD 180
           K  KRDEDEED +EERDQKKV+                           LS SSL PKLD
Sbjct: 160 KVRKRDEDEEDGIEERDQKKVE---------------------------LSASSLLPKLD 219

Query: 181 LVLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYG 240
           LVLTASNIKTKYKDATLLPKWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR  
Sbjct: 220 LVLTASNIKTKYKDATLLPKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR-- 279

Query: 241 IVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFIS 300
            VGAEAWSLMYPSIPQK QSLY++GYKLVIFTNESNIERWKNKRQVAVDSKLGRL++FIS
Sbjct: 280 -VGAEAWSLMYPSIPQKLQSLYNEGYKLVIFTNESNIERWKNKRQVAVDSKLGRLDSFIS 339

Query: 301 QVNVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAG 360
           +VNVPVQVFVACGIGSG GKSEEDP+RKPKPGMW IMENHFNGGIP+N+DQCFYVGDAAG
Sbjct: 340 RVNVPVQVFVACGIGSGSGKSEEDPFRKPKPGMWQIMENHFNGGIPINLDQCFYVGDAAG 387

Query: 361 RAKDHSDADLKFAQAIGLKFYVPEEFFVE 388
           RAKDHSDADL+FAQAIGLKFYVPEEFFVE
Sbjct: 400 RAKDHSDADLRFAQAIGLKFYVPEEFFVE 387

[Close]

BLAST of Lsi04G020110 vs. NCBI nr
Match: XP_008463743.1 (PREDICTED: polynucleotide 3'-phosphatase ZDP [Cucumis melo])

HSP 1 Score: 580.1 bits (1494), Expect = 1.4e-161
Identity = 305/387 (78.81%), Postives = 324/387 (83.72%), Query Frame = 0


Query: 1   MSPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSD 60
           MS SS+KIVAEYAKSNRSTCKNCSKTIPA TLRLGFVTRDGRGFDMTRWFHS CASFGS 
Sbjct: 40  MSSSSLKIVAEYAKSNRSTCKNCSKTIPASTLRLGFVTRDGRGFDMTRWFHSHCASFGSH 99

Query: 61  PVSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCRSHESKG 120
           PVSSAE+INGFALL+                           SDQEALK++VCRSHESK 
Sbjct: 100 PVSSAEDINGFALLK--------------------------DSDQEALKKMVCRSHESKV 159

Query: 121 CKRDEDEEDRVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTSSLQPKLDLV 180
            KRD DE+D VEERDQKKVKV  QK     +  + + V   +     LSTSSLQPKLDLV
Sbjct: 160 HKRDGDEKDGVEERDQKKVKV-HQK-----DGDEEYGVEERDQKNVKLSTSSLQPKLDLV 219

Query: 181 LTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLANTSVKRYGIV 240
           LTAS+IKTKYKDATLL KWKAFQTLIFLE+DDGLHHSSKIAAFDFDGCLANTSVKR   V
Sbjct: 220 LTASDIKTKYKDATLLAKWKAFQTLIFLEQDDGLHHSSKIAAFDFDGCLANTSVKR---V 279

Query: 241 GAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 300
           GAEAWSLMYPSIPQK QSL+DDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV
Sbjct: 280 GAEAWSLMYPSIPQKLQSLHDDGYKLVIFTNESNIERWKNKRQVAVDSKLGRLNNFISQV 339

Query: 301 NVPVQVFVACGIGSGLGKSEEDPYRKPKPGMWHIMENHFNGGIPVNIDQCFYVGDAAGRA 360
            VPVQVFVACGIGS  GKSEED +RKPKPGMWHIME+HFNGG+P++IDQCFYVGDAAGRA
Sbjct: 340 KVPVQVFVACGIGSKSGKSEEDTFRKPKPGMWHIMESHFNGGVPIDIDQCFYVGDAAGRA 391

Query: 361 KDHSDADLKFAQAIGLKFYVPEEFFVE 388
           KDHSDAD+KFAQAIGLKFYVPEEFFVE
Sbjct: 400 KDHSDADIKFAQAIGLKFYVPEEFFVE 391

[Close]

BLAST of Lsi04G020110 vs. TAIR 10
Match: AT3G14890.1 (phosphoesterase )

HSP 1 Score: 403.7 bits (1036), Expect = 1.7e-112
Identity = 211/396 (53.28%), Postives = 273/396 (68.94%), Query Frame = 0


Query: 2   SPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSDP 61
           SP S K+++EYAKS+RSTCK CS+TI AK LRLG VTR+ RGFDM +W H  C    SDP
Sbjct: 322 SPDSSKVISEYAKSSRSTCKKCSQTIAAKELRLGLVTRNFRGFDMKQWHHLGCFPVDSDP 381

Query: 62  VSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCR-SHESKG 121
           + S E+I GF+ L++                           DQ+ALK+LV +   ++  
Sbjct: 382 IVSVEDIGGFSELQS--------------------------GDQDALKELVQQCGKQTLV 441

Query: 122 CKRDEDEED---------RVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTS 181
            K DED +D            +R   +V   +++     +  Q  Q+   + +  + STS
Sbjct: 442 DKMDEDNDDTEAKIKLTEETNKRKHSEVGEMVEEDESLTKAKQ--QMAKTHKVNMSESTS 501

Query: 182 SLQPKLDLVLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLAN 241
            ++ + ++ L+AS++K KY+DA LLPKWKAF+T+IFLE DDGL+ S KIAAFDFDGCLA 
Sbjct: 502 QVEVEAEITLSASDVKDKYRDANLLPKWKAFETVIFLERDDGLNDSEKIAAFDFDGCLAK 561

Query: 242 TSVKRYGIVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLG 301
           TSVK   IVGA+AWSLMYPSIP+K QSL+D GYKLVIFTNESNI+RWKNKRQ AVDSK+G
Sbjct: 562 TSVK---IVGADAWSLMYPSIPEKLQSLHDQGYKLVIFTNESNIDRWKNKRQAAVDSKIG 621

Query: 302 RLNNFISQVNVPVQVFVACGIGSGLGK-SEEDPYRKPKPGMWHIMENHFNGGIPVNIDQC 361
           RLN+FI +V VP+QVF+ACG+ S  GK  ++D YRKPK GMW +M+ HFN GI +++D+ 
Sbjct: 622 RLNSFIERVKVPIQVFIACGVSSSGGKGGKDDLYRKPKAGMWQLMKKHFNSGIAIDMDKS 681

Query: 362 FYVGDAAGRAKDHSDADLKFAQAIGLKFYVPEEFFV 387
           FYVGDAAGR  DHSDAD+KFAQA GLKF+ PEE+F+
Sbjct: 682 FYVGDAAGRKMDHSDADIKFAQASGLKFFTPEEYFI 686

[Close]

BLAST of Lsi04G020110 vs. TAIR 10
Match: AT3G14890.2 (phosphoesterase )

HSP 1 Score: 403.7 bits (1036), Expect = 1.7e-112
Identity = 211/396 (53.28%), Postives = 273/396 (68.94%), Query Frame = 0


Query: 2   SPSSVKIVAEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFDMTRWFHSDCASFGSDP 61
           SP S K+++EYAKS+RSTCK CS+TI AK LRLG VTR+ RGFDM +W H  C    SDP
Sbjct: 312 SPDSSKVISEYAKSSRSTCKKCSQTIAAKELRLGLVTRNFRGFDMKQWHHLGCFPVDSDP 371

Query: 62  VSSAEEINGFALLEACLNTKPYNSLCSSSERDSFFTESPHGSDQEALKQLVCR-SHESKG 121
           + S E+I GF+ L++                           DQ+ALK+LV +   ++  
Sbjct: 372 IVSVEDIGGFSELQS--------------------------GDQDALKELVQQCGKQTLV 431

Query: 122 CKRDEDEED---------RVEERDQKKVKVCIQKPTPFAERFQHFQVILVNSIVDTLSTS 181
            K DED +D            +R   +V   +++     +  Q  Q+   + +  + STS
Sbjct: 432 DKMDEDNDDTEAKIKLTEETNKRKHSEVGEMVEEDESLTKAKQ--QMAKTHKVNMSESTS 491

Query: 182 SLQPKLDLVLTASNIKTKYKDATLLPKWKAFQTLIFLEEDDGLHHSSKIAAFDFDGCLAN 241
            ++ + ++ L+AS++K KY+DA LLPKWKAF+T+IFLE DDGL+ S KIAAFDFDGCLA 
Sbjct: 492 QVEVEAEITLSASDVKDKYRDANLLPKWKAFETVIFLERDDGLNDSEKIAAFDFDGCLAK 551

Query: 242 TSVKRYGIVGAEAWSLMYPSIPQKFQSLYDDGYKLVIFTNESNIERWKNKRQVAVDSKLG 301
           TSVK   IVGA+AWSLMYPSIP+K QSL+D GYKLVIFTNESNI+RWKNKRQ AVDSK+G
Sbjct: 552 TSVK---IVGADAWSLMYPSIPEKLQSLHDQGYKLVIFTNESNIDRWKNKRQAAVDSKIG 611

Query: 302 RLNNFISQVNVPVQVFVACGIGSGLGK-SEEDPYRKPKPGMWHIMENHFNGGIPVNIDQC 361
           RLN+FI +V VP+QVF+ACG+ S  GK  ++D YRKPK GMW +M+ HFN GI +++D+ 
Sbjct: 612 RLNSFIERVKVPIQVFIACGVSSSGGKGGKDDLYRKPKAGMWQLMKKHFNSGIAIDMDKS 671

Query: 362 FYVGDAAGRAKDHSDADLKFAQAIGLKFYVPEEFFV 387
           FYVGDAAGR  DHSDAD+KFAQA GLKF+ PEE+F+
Sbjct: 672 FYVGDAAGRKMDHSDADIKFAQASGLKFFTPEEYFI 676

[Close]

BLAST of Lsi04G020110 vs. TAIR 10
Match: AT2G31320.1 (poly(ADP-ribose) polymerase 2 )

HSP 1 Score: 45.4 bits (106), Expect = 1.2e-04
Identity = 24/67 (35.82%), Postives = 35/67 (52.24%), Query Frame = 0


Query: 10 AEYAKSNRSTCKNCSKTIPAKTLRLGFVTRDGRGFD--MTRWFHSDCASFGSDPVSSAEE 69
          AEYAKS+RS+CK C   I  +  RLG + +    FD  M  W H+ C    +  + S ++
Sbjct: 10 AEYAKSSRSSCKTCKSVINKENFRLGKLVQSTH-FDGIMPMWNHASCILKKTKQIKSVDD 69

Query: 70 INGFALL 75
          + G   L
Sbjct: 70 VEGIESL 75

The following BLAST results are available for this feature:

BLAST of Lsi04G020110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84JE8	2.4e-111	53.28	Polynucleotide 3'-phosphatase ZDP OS=Arabidopsis thaliana OX=3702 GN=ZDP PE=1 SV...	[more]
Q9JLV6	2.1e-27	36.87	Bifunctional polynucleotide phosphatase/kinase OS=Mus musculus OX=10090 GN=Pnkp ...	[more]
Q96T60	4.8e-27	36.00	Bifunctional polynucleotide phosphatase/kinase OS=Homo sapiens OX=9606 GN=PNKP P...	[more]
Q19683	1.0e-24	38.51	Uncharacterized protein F21D5.5 OS=Caenorhabditis elegans OX=6239 GN=F21D5.5 PE=...	[more]
O13911	1.2e-22	34.97	Bifunctional polynucleotide phosphatase/kinase OS=Schizosaccharomyces pombe (str...	[more]

BLAST of Lsi04G020110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZI4	7.4e-164	78.97	PARP-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G627160 P...	[more]
A0A1S3CLI9	6.9e-162	78.81	polynucleotide 3'-phosphatase ZDP OS=Cucumis melo OX=3656 GN=LOC103501815 PE=4 S...	[more]
A0A6J1IH48	1.5e-161	77.26	polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1IFZ5	1.5e-161	77.26	polynucleotide 3'-phosphatase ZDP isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1F9Q9	2.6e-161	77.26	polynucleotide 3'-phosphatase ZDP isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of Lsi04G020110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146024.2	1.5e-163	78.97	polynucleotide 3'-phosphatase ZDP isoform X1 [Cucumis sativus] >KGN55065.1 hypot...	[more]
KAG6591920.1	3.4e-163	78.29	Polynucleotide 3'-phosphatase ZDP, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038898753.1	2.2e-162	78.87	polynucleotide 3'-phosphatase ZDP [Benincasa hispida]	[more]
KAG7024794.1	4.9e-162	78.66	Polynucleotide 3'-phosphatase ZDP, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_008463743.1	1.4e-161	78.81	PREDICTED: polynucleotide 3'-phosphatase ZDP [Cucumis melo]	[more]

BLAST of Lsi04G020110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14890.1	1.7e-112	53.28	phosphoesterase	[more]
AT3G14890.2	1.7e-112	53.28	phosphoesterase	[more]
AT2G31320.1	1.2e-04	35.82	poly(ADP-ribose) polymerase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001510	Zinc finger, PARP-type	SMART	SM01336	zf_PARP_3	coord: 11..79 e-value: 5.3E-13 score: 59.2
IPR001510	Zinc finger, PARP-type	PFAM	PF00645	zf-PARP	coord: 11..58 e-value: 3.1E-11 score: 43.8
IPR001510	Zinc finger, PARP-type	PROSITE	PS50064	PARP_ZN_FINGER_2	coord: 8..75 score: 15.775247
IPR036957	Zinc finger, PARP-type superfamily	GENE3D	3.30.1740.10		coord: 2..82 e-value: 8.1E-16 score: 60.3
IPR006551	Polynucleotide 3'-phosphatase	TIGRFAM	TIGR01664	TIGR01664	coord: 207..385 e-value: 5.5E-56 score: 187.1
IPR006549	HAD-superfamily hydrolase,subfamily IIIA	TIGRFAM	TIGR01662	TIGR01662	coord: 219..378 e-value: 1.8E-34 score: 116.8
IPR013954	Polynucleotide kinase 3 phosphatase	PFAM	PF08645	PNK3P	coord: 219..385 e-value: 1.4E-44 score: 151.7
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 197..387 e-value: 1.2E-54 score: 186.4
None	No IPR available	PANTHER	PTHR12083	BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE	coord: 2..386
None	No IPR available	CDD	cd01625	HAD_PNP	coord: 219..385 e-value: 2.4097E-69 score: 213.366
None	No IPR available	SUPERFAMILY	57716	Glucocorticoid receptor-like (DNA-binding domain)	coord: 7..76
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 204..385

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G020110.1	Lsi04G020110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0046939	nucleotide phosphorylation
biological_process	GO:0098506	polynucleotide 3' dephosphorylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003690	double-stranded DNA binding
molecular_function	GO:0046404	polydeoxyribonucleotide 5'-hydroxyl-kinase activity
molecular_function	GO:0046403	polynucleotide 3'-phosphatase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003677	DNA binding