Lsi04G018300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G018300
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-H2 finger protein ATL80
Location	chr04: 25437672 .. 25439076 (+)
RNA-Seq Expression	Lsi04G018300
Synteny	Lsi04G018300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACGCCGTTAGAATTGAGGTGGGTGAGGCTAATGAGAAGAAGCTCCGAAAGCGGAAAAACGCAAGCCCGATTCTCGTGTTCAGTATAAAATCCCTTCGAAACGACAAACGTTACGCCCACGGTTGATTCAAAACGACTATCCTTCTCATCTCTTCTTTTATCTTTATAGACTCTTCCTTTCCTCTCGCCGCTTCAAATGCGTCCCTTCAGATATCTTGCCGGCGTCAACTCCTCCGCCATCTCCGACACCGTCGAGCCTGAAAGGTATGACTCTGACTTCGTCATAATCCTTGCCGCCCTCCTCTGCGCTTTGATTTGCGTTCTCGGTCTTGTTGCTGTTGCTCGCTGCGCCTGGCTACGACACCTCGCCGGCGATGGCGGCGGCGCTGCTTCCACTCGTCCTCCTCCACCTCCTGCCTCCAACAAAGGTCTCAAGAAGAAGATCCTGAGGTCTCTTCCGAAGTACACCTTCACCGCTGAGTTTTCTGCTCAGTTCTCAGATTGCGCGATCTGCCTCGCGGAGTTCGCTGTCGGTGATGAAATCCGTGTGTTGCCGCAGTGTGGCCATGGATTTCACATGTCCTGCATCGATACGTGGTTCCGATCTCACTCTTCCTGTCCTTCCTGCCGTCAGATTCTGGTGGTAAGTCAGTGCCAGAAGTGCGGCGGTTTTCCGGCAAGCTCGAGTTCGAACGGTGGAACGGAATACAGAATGAAAGAGGAAGGAGATGATAAGACGAATAGGTTCTTACCTTAACCCTAGGGTTTTTCTCTTAACTACTGCGATTCTTGTTCCTTCCTTCCTTCCTCTGTGTTGTAAATAGCTTTATTAGTCAAATTCATGTAATTACTGAAAATTGTGGATGTATTCAAGTTGTAAACGAAGGCGATTCCCCTCAATTTGAAATCAGCCGTTCTGATAGTTCTGTTTCTGTTGTGAATATTGACTTCAAATCTGTCGTCGATTTTCAGCCGAGATGGCATAACTTTACAGTACAGGCGATTAATTCGAAAAGGGGAACAGTATCAGTATCAGTATCATCAGTATCATGTGGATGATGAACATTGCAACTGCCAATGTGATTTGTTTATTTCCATTTTGGATCGCATTTTTCAGCAGCAAATGGCTCCTCCTCTCTTCATCTCTCTTCACATTGCCAATTTTTCTCTCTCCTCCGCCCTGAAGATTTAAGAGGTCAGATCCATAAAGTTCTGAAAATGGAATAATTCTGCAACTGCAACTGCTCCATTTTCTAATTAAAAATTAGCAAAATCCCTAATCCAAATTTCACCATCCATAGCCATGTCTGCTCTCTCTCCTTTTCACTTTCACTTATCTTCTCACCATGATAATCACTATCAAATTTTTTAGGTTTAAAATTAATTTTCATCTCTAAACTTTCC

mRNA sequence

AAACGCCGTTAGAATTGAGGTGGGTGAGGCTAATGAGAAGAAGCTCCGAAAGCGGAAAAACGCAAGCCCGATTCTCGTGTTCAGTATAAAATCCCTTCGAAACGACAAACGTTACGCCCACGGTTGATTCAAAACGACTATCCTTCTCATCTCTTCTTTTATCTTTATAGACTCTTCCTTTCCTCTCGCCGCTTCAAATGCGTCCCTTCAGATATCTTGCCGGCGTCAACTCCTCCGCCATCTCCGACACCGTCGAGCCTGAAAGGTATGACTCTGACTTCGTCATAATCCTTGCCGCCCTCCTCTGCGCTTTGATTTGCGTTCTCGGTCTTGTTGCTGTTGCTCGCTGCGCCTGGCTACGACACCTCGCCGGCGATGGCGGCGGCGCTGCTTCCACTCGTCCTCCTCCACCTCCTGCCTCCAACAAAGGTCTCAAGAAGAAGATCCTGAGGTCTCTTCCGAAGTACACCTTCACCGCTGAGTTTTCTGCTCAGTTCTCAGATTGCGCGATCTGCCTCGCGGAGTTCGCTGTCGGTGATGAAATCCGTGTGTTGCCGCAGTGTGGCCATGGATTTCACATGTCCTGCATCGATACGTGGTTCCGATCTCACTCTTCCTGTCCTTCCTGCCGTCAGATTCTGGTGGTAAGTCAGTGCCAGAAGTGCGGCGGTTTTCCGGCAAGCTCGAGTTCGAACGGTGGAACGGAATACAGAATGAAAGAGGAAGGAGATGATAAGACGAATAGCCGAGATGGCATAACTTTACAGTACAGGCGATTAATTCGAAAAGGGGAACAGTATCAGTATCAGTATCATCAGTATCATGTGGATGATGAACATTGCAACTGCCAATGTGATTTGTTTATTTCCATTTTGGATCGCATTTTTCAGCAGCAAATGGCTCCTCCTCTCTTCATCTCTCTTCACATTGCCAATTTTTCTCTCTCCTCCGCCCTGAAGATTTAAGAGGTCAGATCCATAAAGTTCTGAAAATGGAATAATTCTGCAACTGCAACTGCTCCATTTTCTAATTAAAAATTAGCAAAATCCCTAATCCAAATTTCACCATCCATAGCCATGTCTGCTCTCTCTCCTTTTCACTTTCACTTATCTTCTCACCATGATAATCACTATCAAATTTTTTAGGTTTAAAATTAATTTTCATCTCTAAACTTTCC

Coding sequence (CDS)

ATGCGTCCCTTCAGATATCTTGCCGGCGTCAACTCCTCCGCCATCTCCGACACCGTCGAGCCTGAAAGGTATGACTCTGACTTCGTCATAATCCTTGCCGCCCTCCTCTGCGCTTTGATTTGCGTTCTCGGTCTTGTTGCTGTTGCTCGCTGCGCCTGGCTACGACACCTCGCCGGCGATGGCGGCGGCGCTGCTTCCACTCGTCCTCCTCCACCTCCTGCCTCCAACAAAGGTCTCAAGAAGAAGATCCTGAGGTCTCTTCCGAAGTACACCTTCACCGCTGAGTTTTCTGCTCAGTTCTCAGATTGCGCGATCTGCCTCGCGGAGTTCGCTGTCGGTGATGAAATCCGTGTGTTGCCGCAGTGTGGCCATGGATTTCACATGTCCTGCATCGATACGTGGTTCCGATCTCACTCTTCCTGTCCTTCCTGCCGTCAGATTCTGGTGGTAAGTCAGTGCCAGAAGTGCGGCGGTTTTCCGGCAAGCTCGAGTTCGAACGGTGGAACGGAATACAGAATGAAAGAGGAAGGAGATGATAAGACGAATAGCCGAGATGGCATAACTTTACAGTACAGGCGATTAATTCGAAAAGGGGAACAGTATCAGTATCAGTATCATCAGTATCATGTGGATGATGAACATTGCAACTGCCAATGTGATTTGTTTATTTCCATTTTGGATCGCATTTTTCAGCAGCAAATGGCTCCTCCTCTCTTCATCTCTCTTCACATTGCCAATTTTTCTCTCTCCTCCGCCCTGAAGATTTAA

Protein sequence

MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLPQCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDKTNSRDGITLQYRRLIRKGEQYQYQYHQYHVDDEHCNCQCDLFISILDRIFQQQMAPPLFISLHIANFSLSSALKI

Homology

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BLAST of Lsi04G018300 vs. ExPASy Swiss-Prot
Match: Q8LC69 (RING-H2 finger protein ATL8 OS=Arabidopsis thaliana OX=3702 GN=ATL8 PE=2 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 1.7e-52
Identity = 104/181 (57.46%), Postives = 132/181 (72.93%), Query Frame = 0


Query: 4   FRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGG 63
           FR L   NS++ ++   P  ++SD V+ILA LLCAL C++GL+AV+RCAWLR +A     
Sbjct: 6   FRLLQEANSTSPAEASPP--FNSDLVLILAVLLCALTCIIGLIAVSRCAWLRRIA-SRNR 65

Query: 64  AASTRPPPPPASNKGLKKKILRSLPKYTFTAEF--SAQFSDCAICLAEFAVGDEIRVLPQ 123
           +  T PPP  A+NKGLKKK+LRSLPK T++ +   + +  +CAICL EFA GDE+RVLPQ
Sbjct: 66  SDQTHPPPVAAANKGLKKKVLRSLPKLTYSPDSPPAEKLVECAICLTEFAAGDELRVLPQ 125

Query: 124 CGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDKT 183
           CGHGFH+SCIDTW  SHSSCPSCRQILVV++C KCGG P SSSS    + R+K+  D   
Sbjct: 126 CGHGFHVSCIDTWLGSHSSCPSCRQILVVTRCHKCGGLPGSSSSGPEPDTRIKQREDGPD 183

[Close]

BLAST of Lsi04G018300 vs. ExPASy Swiss-Prot
Match: Q9LM69 (RING-H2 finger protein ATL80 OS=Arabidopsis thaliana OX=3702 GN=ATL80 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.8e-52
Identity = 108/189 (57.14%), Postives = 135/189 (71.43%), Query Frame = 0


Query: 4   FRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD--- 63
           FR L   N+ + +        +SD V+ILAALLCALICVLGL+AV+RC WLR LA     
Sbjct: 6   FRLLVESNTPSPAIDNSTAALNSDLVVILAALLCALICVLGLIAVSRCVWLRRLAAGNRT 65

Query: 64  -GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEF--SAQFSDCAICLAEFAVGDEIR 123
             G    +  PP  A+NKGLKKK+L+SLPK TF+ E   S +F++CAICLAEF+ GDE+R
Sbjct: 66  VSGSQTQSPQPPVAAANKGLKKKVLQSLPKLTFSPESPESEKFAECAICLAEFSAGDELR 125

Query: 124 VLPQCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMK--- 183
           VLPQCGHGFH++CIDTW  SHSSCPSCRQILVV++C KCGG P SSSS   +E  ++   
Sbjct: 126 VLPQCGHGFHVACIDTWLGSHSSCPSCRQILVVARCHKCGGLPGSSSSGLESEPEIEIRI 185

[Close]

BLAST of Lsi04G018300 vs. ExPASy Swiss-Prot
Match: Q9ZT49 (Probable E3 ubiquitin-protein ligase ATL45 OS=Arabidopsis thaliana OX=3702 GN=ATL45 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 7.5e-37
Identity = 78/149 (52.35%), Postives = 97/149 (65.10%), Query Frame = 0


Query: 25  DSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGGAASTRPPPPPASNKGLKKKIL 84
           ++D V+IL+ALLCAL+CV GL AVARCAWLR L G    A    PPP    NKGLKKK L
Sbjct: 23  ETDMVVILSALLCALVCVAGLAAVARCAWLRRLTGVNPAAVGEAPPP----NKGLKKKAL 82

Query: 85  RSLPKYTFTAEFSA----------------QFSDCAICLAEFAVGDEIRVLPQCGHGFHM 144
           ++LPK T+TA  S                   ++CAIC+ EF+ G+EIR+LP C H FH+
Sbjct: 83  QALPKSTYTASASTAAAADDLPCSSVGDGDSSTECAICITEFSEGEEIRILPLCSHAFHV 142

Query: 145 SCIDTWFRSHSSCPSCRQILVVSQCQKCG 158
           +CID W  S SSCPSCR+ILV  +C +CG
Sbjct: 143 ACIDKWLTSRSSCPSCRRILVPVKCDRCG 167

[Close]

BLAST of Lsi04G018300 vs. ExPASy Swiss-Prot
Match: O22755 (Probable E3 ubiquitin-protein ligase ATL44 OS=Arabidopsis thaliana OX=3702 GN=ATL44 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.4e-35
Identity = 78/141 (55.32%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 25  DSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGGAASTRPPPPPASNKGLKKKIL 84
           +SD V+IL+ALLCALICV GL AV RCAWLR     G           P+ NKGLKKK L
Sbjct: 25  ESDMVVILSALLCALICVAGLAAVVRCAWLRRFTAGGDS---------PSPNKGLKKKAL 84

Query: 85  RSLPKYTFTAEFS--------AQFSDCAICLAEFAVGDEIRVLPQCGHGFHMSCIDTWFR 144
           +SLP+ TFTA  S           ++CAICL +FA G+EIRVLP CGH FH+ CID W  
Sbjct: 85  QSLPRSTFTAAESTSGAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLV 144

Query: 145 SHSSCPSCRQILVVSQCQKCG 158
           S SSCPSCR+IL   +C +CG
Sbjct: 145 SRSSCPSCRRILTPVRCDRCG 156

[Close]

BLAST of Lsi04G018300 vs. ExPASy Swiss-Prot
Match: Q9LZV8 (RING-H2 finger protein ATL74 OS=Arabidopsis thaliana OX=3702 GN=ATL74 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.6e-25
Identity = 63/127 (49.61%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 24  YDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGGAASTRPPPPPASNKGLKKKI 83
           +D++ VIILAALLCALI  LGL ++ RCA        G G +S+      A   GLKK+ 
Sbjct: 30  FDANMVIILAALLCALILALGLNSILRCA-----MRCGFGLSSSAAAGTVADRAGLKKRE 89

Query: 84  LRSLPKYTF-TAEFSAQFSDCAICLAEFAVGDEIRVLPQCGHGFHMSCIDTWFRSHSSCP 143
           L+  P   + + E     ++CAICL EFA G+ +RVLP C H FHMSCIDTW  SHSSCP
Sbjct: 90  LKKFPVAEYGSGEVKIAATECAICLGEFADGERVRVLPPCNHSFHMSCIDTWLVSHSSCP 149

Query: 144 SCRQILV 150
           +CR  L+
Sbjct: 150 NCRHSLI 151

[Close]

BLAST of Lsi04G018300 vs. ExPASy TrEMBL
Match: A0A0A0L5Y2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G664340 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 8.2e-95
Identity = 176/182 (96.70%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLAGVNSSA SDT EP RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLAGVNSSATSDTFEP-RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           GGGA STRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP
Sbjct: 61  GGGAGSTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+KEEGDDK
Sbjct: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKEEGDDK 180

Query: 181 TN 183
           TN
Sbjct: 181 TN 181

[Close]

BLAST of Lsi04G018300 vs. ExPASy TrEMBL
Match: A0A5A7VGL3 (RING-H2 finger protein ATL80 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00440 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.2e-93
Identity = 174/182 (95.60%), Postives = 176/182 (96.70%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLAGVNSSA SDT EP RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLAGVNSSATSDTFEP-RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           G GA STRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP
Sbjct: 61  GAGAGSTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFH+SCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+KEEGDDK
Sbjct: 121 QCGHGFHVSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKEEGDDK 180

Query: 181 TN 183
           TN
Sbjct: 181 TN 181

[Close]

BLAST of Lsi04G018300 vs. ExPASy TrEMBL
Match: A0A1S3BWC1 (RING-H2 finger protein ATL80 OS=Cucumis melo OX=3656 GN=LOC103493842 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.2e-93
Identity = 174/182 (95.60%), Postives = 176/182 (96.70%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLAGVNSSA SDT EP RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLAGVNSSATSDTFEP-RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           G GA STRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP
Sbjct: 61  GAGAGSTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFH+SCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+KEEGDDK
Sbjct: 121 QCGHGFHVSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKEEGDDK 180

Query: 181 TN 183
           TN
Sbjct: 181 TN 181

[Close]

BLAST of Lsi04G018300 vs. ExPASy TrEMBL
Match: A0A6J1FNK0 (RING-H2 finger protein ATL80-like OS=Cucurbita moschata OX=3662 GN=LOC111446793 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 4.5e-93
Identity = 170/182 (93.41%), Postives = 176/182 (96.70%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MR FRYLA  NSS+ISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRSFRYLADANSSSISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEF+AQFSDCAICLAEF VGDEIRVLP
Sbjct: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFAAQFSDCAICLAEFVVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFH+SCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+K+EG+DK
Sbjct: 121 QCGHGFHVSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKDEGEDK 180

Query: 181 TN 183
            N
Sbjct: 181 RN 182

[Close]

BLAST of Lsi04G018300 vs. ExPASy TrEMBL
Match: A0A6J1IVW2 (RING-H2 finger protein ATL80 OS=Cucurbita maxima OX=3661 GN=LOC111481072 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.8e-92
Identity = 170/182 (93.41%), Postives = 176/182 (96.70%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLA  NSS+ISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLADANSSSISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEF+AQFSDCAICLAEF VGDEIRVLP
Sbjct: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFAAQFSDCAICLAEFVVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFH+SCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+K +G+DK
Sbjct: 121 QCGHGFHVSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVK-DGEDK 180

Query: 181 TN 183
            N
Sbjct: 181 RN 181

[Close]

BLAST of Lsi04G018300 vs. NCBI nr
Match: XP_038898612.1 (RING-H2 finger protein ATL8 [Benincasa hispida])

HSP 1 Score: 375.2 bits (962), Expect = 4.6e-100
Identity = 185/193 (95.85%), Postives = 186/193 (96.37%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLAGVNSSAISDT EPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLAGVNSSAISDTPEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           GGGAASTR PPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEF VGDEIRVLP
Sbjct: 61  GGGAASTRSPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFVVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFHMSCID WFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+KEEGDDK
Sbjct: 121 QCGHGFHMSCIDMWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKEEGDDK 180

Query: 181 TNSRDGITLQYRR 194
           TNS DGIT QYRR
Sbjct: 181 TNSGDGITSQYRR 193

[Close]

BLAST of Lsi04G018300 vs. NCBI nr
Match: XP_004145558.1 (RING-H2 finger protein ATL8 [Cucumis sativus] >KGN55526.1 hypothetical protein Csa_012171 [Cucumis sativus])

HSP 1 Score: 356.7 bits (914), Expect = 1.7e-94
Identity = 176/182 (96.70%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLAGVNSSA SDT EP RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLAGVNSSATSDTFEP-RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           GGGA STRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP
Sbjct: 61  GGGAGSTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+KEEGDDK
Sbjct: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKEEGDDK 180

Query: 181 TN 183
           TN
Sbjct: 181 TN 181

[Close]

BLAST of Lsi04G018300 vs. NCBI nr
Match: XP_008453009.1 (PREDICTED: RING-H2 finger protein ATL80 [Cucumis melo] >KAA0064659.1 RING-H2 finger protein ATL80 [Cucumis melo var. makuwa] >TYK19932.1 RING-H2 finger protein ATL80 [Cucumis melo var. makuwa])

HSP 1 Score: 352.8 bits (904), Expect = 2.4e-93
Identity = 174/182 (95.60%), Postives = 176/182 (96.70%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLAGVNSSA SDT EP RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLAGVNSSATSDTFEP-RYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           G GA STRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP
Sbjct: 61  GAGAGSTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFH+SCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+KEEGDDK
Sbjct: 121 QCGHGFHVSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKEEGDDK 180

Query: 181 TN 183
           TN
Sbjct: 181 TN 181

[Close]

BLAST of Lsi04G018300 vs. NCBI nr
Match: KAG6608649.1 (RING-H2 finger protein ATL80, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037966.1 RING-H2 finger protein ATL80, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 351.3 bits (900), Expect = 7.1e-93
Identity = 170/182 (93.41%), Postives = 176/182 (96.70%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MRPFRYLA  NSS+ISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRPFRYLADANSSSISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEF+AQFSDCAICLAEF VGDEIRVLP
Sbjct: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFAAQFSDCAICLAEFVVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFH+SCIDTWFRSHSSCPSCRQILV SQCQKCGGFPASSSSNGGTE R+K+EG+DK
Sbjct: 121 QCGHGFHVSCIDTWFRSHSSCPSCRQILVGSQCQKCGGFPASSSSNGGTESRVKDEGEDK 180

Query: 181 TN 183
            N
Sbjct: 181 RN 182

[Close]

BLAST of Lsi04G018300 vs. NCBI nr
Match: XP_022941514.1 (RING-H2 finger protein ATL80-like [Cucurbita moschata])

HSP 1 Score: 350.9 bits (899), Expect = 9.2e-93
Identity = 170/182 (93.41%), Postives = 176/182 (96.70%), Query Frame = 0


Query: 1   MRPFRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60
           MR FRYLA  NSS+ISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD
Sbjct: 1   MRSFRYLADANSSSISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD 60

Query: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFSAQFSDCAICLAEFAVGDEIRVLP 120
           GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEF+AQFSDCAICLAEF VGDEIRVLP
Sbjct: 61  GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEFAAQFSDCAICLAEFVVGDEIRVLP 120

Query: 121 QCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDK 180
           QCGHGFH+SCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTE R+K+EG+DK
Sbjct: 121 QCGHGFHVSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTESRVKDEGEDK 180

Query: 181 TN 183
            N
Sbjct: 181 RN 182

[Close]

BLAST of Lsi04G018300 vs. TAIR 10
Match: AT1G76410.1 (RING/U-box superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.2e-53
Identity = 104/181 (57.46%), Postives = 132/181 (72.93%), Query Frame = 0


Query: 4   FRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGG 63
           FR L   NS++ ++   P  ++SD V+ILA LLCAL C++GL+AV+RCAWLR +A     
Sbjct: 6   FRLLQEANSTSPAEASPP--FNSDLVLILAVLLCALTCIIGLIAVSRCAWLRRIA-SRNR 65

Query: 64  AASTRPPPPPASNKGLKKKILRSLPKYTFTAEF--SAQFSDCAICLAEFAVGDEIRVLPQ 123
           +  T PPP  A+NKGLKKK+LRSLPK T++ +   + +  +CAICL EFA GDE+RVLPQ
Sbjct: 66  SDQTHPPPVAAANKGLKKKVLRSLPKLTYSPDSPPAEKLVECAICLTEFAAGDELRVLPQ 125

Query: 124 CGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMKEEGDDKT 183
           CGHGFH+SCIDTW  SHSSCPSCRQILVV++C KCGG P SSSS    + R+K+  D   
Sbjct: 126 CGHGFHVSCIDTWLGSHSSCPSCRQILVVTRCHKCGGLPGSSSSGPEPDTRIKQREDGPD 183

[Close]

BLAST of Lsi04G018300 vs. TAIR 10
Match: AT1G20823.1 (RING/U-box superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.0e-53
Identity = 108/189 (57.14%), Postives = 135/189 (71.43%), Query Frame = 0


Query: 4   FRYLAGVNSSAISDTVEPERYDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGD--- 63
           FR L   N+ + +        +SD V+ILAALLCALICVLGL+AV+RC WLR LA     
Sbjct: 6   FRLLVESNTPSPAIDNSTAALNSDLVVILAALLCALICVLGLIAVSRCVWLRRLAAGNRT 65

Query: 64  -GGGAASTRPPPPPASNKGLKKKILRSLPKYTFTAEF--SAQFSDCAICLAEFAVGDEIR 123
             G    +  PP  A+NKGLKKK+L+SLPK TF+ E   S +F++CAICLAEF+ GDE+R
Sbjct: 66  VSGSQTQSPQPPVAAANKGLKKKVLQSLPKLTFSPESPESEKFAECAICLAEFSAGDELR 125

Query: 124 VLPQCGHGFHMSCIDTWFRSHSSCPSCRQILVVSQCQKCGGFPASSSSNGGTEYRMK--- 183
           VLPQCGHGFH++CIDTW  SHSSCPSCRQILVV++C KCGG P SSSS   +E  ++   
Sbjct: 126 VLPQCGHGFHVACIDTWLGSHSSCPSCRQILVVARCHKCGGLPGSSSSGLESEPEIEIRI 185

[Close]

BLAST of Lsi04G018300 vs. TAIR 10
Match: AT4G35480.1 (RING-H2 finger A3B )

HSP 1 Score: 155.6 bits (392), Expect = 5.3e-38
Identity = 78/149 (52.35%), Postives = 97/149 (65.10%), Query Frame = 0


Query: 25  DSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGGAASTRPPPPPASNKGLKKKIL 84
           ++D V+IL+ALLCAL+CV GL AVARCAWLR L G    A    PPP    NKGLKKK L
Sbjct: 23  ETDMVVILSALLCALVCVAGLAAVARCAWLRRLTGVNPAAVGEAPPP----NKGLKKKAL 82

Query: 85  RSLPKYTFTAEFSA----------------QFSDCAICLAEFAVGDEIRVLPQCGHGFHM 144
           ++LPK T+TA  S                   ++CAIC+ EF+ G+EIR+LP C H FH+
Sbjct: 83  QALPKSTYTASASTAAAADDLPCSSVGDGDSSTECAICITEFSEGEEIRILPLCSHAFHV 142

Query: 145 SCIDTWFRSHSSCPSCRQILVVSQCQKCG 158
           +CID W  S SSCPSCR+ILV  +C +CG
Sbjct: 143 ACIDKWLTSRSSCPSCRRILVPVKCDRCG 167

[Close]

BLAST of Lsi04G018300 vs. TAIR 10
Match: AT2G17450.1 (RING-H2 finger A3A )

HSP 1 Score: 151.4 bits (381), Expect = 1.0e-36
Identity = 78/141 (55.32%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 25  DSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGGAASTRPPPPPASNKGLKKKIL 84
           +SD V+IL+ALLCALICV GL AV RCAWLR     G           P+ NKGLKKK L
Sbjct: 25  ESDMVVILSALLCALICVAGLAAVVRCAWLRRFTAGGDS---------PSPNKGLKKKAL 84

Query: 85  RSLPKYTFTAEFS--------AQFSDCAICLAEFAVGDEIRVLPQCGHGFHMSCIDTWFR 144
           +SLP+ TFTA  S           ++CAICL +FA G+EIRVLP CGH FH+ CID W  
Sbjct: 85  QSLPRSTFTAAESTSGAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLV 144

Query: 145 SHSSCPSCRQILVVSQCQKCG 158
           S SSCPSCR+IL   +C +CG
Sbjct: 145 SRSSCPSCRRILTPVRCDRCG 156

[Close]

BLAST of Lsi04G018300 vs. TAIR 10
Match: AT5G01880.1 (RING/U-box superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 4.7e-26
Identity = 63/127 (49.61%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 24  YDSDFVIILAALLCALICVLGLVAVARCAWLRHLAGDGGGAASTRPPPPPASNKGLKKKI 83
           +D++ VIILAALLCALI  LGL ++ RCA        G G +S+      A   GLKK+ 
Sbjct: 30  FDANMVIILAALLCALILALGLNSILRCA-----MRCGFGLSSSAAAGTVADRAGLKKRE 89

Query: 84  LRSLPKYTF-TAEFSAQFSDCAICLAEFAVGDEIRVLPQCGHGFHMSCIDTWFRSHSSCP 143
           L+  P   + + E     ++CAICL EFA G+ +RVLP C H FHMSCIDTW  SHSSCP
Sbjct: 90  LKKFPVAEYGSGEVKIAATECAICLGEFADGERVRVLPPCNHSFHMSCIDTWLVSHSSCP 149

Query: 144 SCRQILV 150
           +CR  L+
Sbjct: 150 NCRHSLI 151

The following BLAST results are available for this feature:

BLAST of Lsi04G018300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LC69	1.7e-52	57.46	RING-H2 finger protein ATL8 OS=Arabidopsis thaliana OX=3702 GN=ATL8 PE=2 SV=2	[more]
Q9LM69	2.8e-52	57.14	RING-H2 finger protein ATL80 OS=Arabidopsis thaliana OX=3702 GN=ATL80 PE=2 SV=1	[more]
Q9ZT49	7.5e-37	52.35	Probable E3 ubiquitin-protein ligase ATL45 OS=Arabidopsis thaliana OX=3702 GN=AT...	[more]
O22755	1.4e-35	55.32	Probable E3 ubiquitin-protein ligase ATL44 OS=Arabidopsis thaliana OX=3702 GN=AT...	[more]
Q9LZV8	6.6e-25	49.61	RING-H2 finger protein ATL74 OS=Arabidopsis thaliana OX=3702 GN=ATL74 PE=2 SV=1	[more]

BLAST of Lsi04G018300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5Y2	8.2e-95	96.70	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G664340 P...	[more]
A0A5A7VGL3	1.2e-93	95.60	RING-H2 finger protein ATL80 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BWC1	1.2e-93	95.60	RING-H2 finger protein ATL80 OS=Cucumis melo OX=3656 GN=LOC103493842 PE=4 SV=1	[more]
A0A6J1FNK0	4.5e-93	93.41	RING-H2 finger protein ATL80-like OS=Cucurbita moschata OX=3662 GN=LOC111446793 ...	[more]
A0A6J1IVW2	3.8e-92	93.41	RING-H2 finger protein ATL80 OS=Cucurbita maxima OX=3661 GN=LOC111481072 PE=4 SV...	[more]

BLAST of Lsi04G018300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898612.1	4.6e-100	95.85	RING-H2 finger protein ATL8 [Benincasa hispida]	[more]
XP_004145558.1	1.7e-94	96.70	RING-H2 finger protein ATL8 [Cucumis sativus] >KGN55526.1 hypothetical protein C...	[more]
XP_008453009.1	2.4e-93	95.60	PREDICTED: RING-H2 finger protein ATL80 [Cucumis melo] >KAA0064659.1 RING-H2 fin...	[more]
KAG6608649.1	7.1e-93	93.41	RING-H2 finger protein ATL80, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_022941514.1	9.2e-93	93.41	RING-H2 finger protein ATL80-like [Cucurbita moschata]	[more]

BLAST of Lsi04G018300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76410.1	1.2e-53	57.46	RING/U-box superfamily protein	[more]
AT1G20823.1	2.0e-53	57.14	RING/U-box superfamily protein	[more]
AT4G35480.1	5.3e-38	52.35	RING-H2 finger A3B	[more]
AT2G17450.1	1.0e-36	55.32	RING-H2 finger A3A	[more]
AT5G01880.1	4.7e-26	49.61	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 103..144 e-value: 1.8E-7 score: 40.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 102..145 e-value: 6.3E-14 score: 52.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 103..145 score: 12.592549
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 72..149 e-value: 1.0E-21 score: 78.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 162..182
None	No IPR available	PANTHER	PTHR45798	RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED	coord: 2..179
None	No IPR available	PANTHER	PTHR45798:SF59	RING-H2 FINGER PROTEIN ATL8	coord: 2..179
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 102..145 e-value: 1.19539E-16 score: 69.6381
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 84..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G018300.1	Lsi04G018300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane