Lsi04G018190 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G018190 vs. ExPASy Swiss-Prot
Match: O42287 (Intersectin-1 OS=Xenopus laevis OX=8355 GN=itsn1 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 5.9e-11
Identity = 46/131 (35.11%), Postives = 66/131 (50.38%), Query Frame = 0

Query: 413 GRITGEQARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPA 472
           G ITG+QARN FL   LP+ VL Q+W L+D +ND  +   EF  A+ L++   +G PLP+
Sbjct: 37  GYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPS 96

Query: 473 VLPNNVMFDETLLSMT-----GQSNIV--HPNATWGPRPGFGQQQPQVTARSMAPTAGLR 532
           +LP+N++     +        G S IV   P A   P P      P +    M+P     
Sbjct: 97  ILPSNMLKQPVAMPAAAVAGFGMSGIVGIPPLAAVAPVP-----MPSIPVVGMSPPLVSS 156

Query: 533 PPTPASKADGA 537
            PT    ++GA
Sbjct: 157 VPTVPPLSNGA 162

BLAST of Lsi04G018190 vs. ExPASy Swiss-Prot
Match: Q54KI4 (Epidermal growth factor receptor substrate 15 homolog OS=Dictyostelium discoideum OX=44689 GN=eps15 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.9e-09
Identity = 32/92 (34.78%), Postives = 49/92 (53.26%), Query Frame = 0

Query: 398 QKYTKVFMEVDTDRDGRITGEQARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFA 457
           Q Y  +F + D D DG I G QA+ +F +  LP ++L  +W+LSD   D  L  +EF  A
Sbjct: 117 QNYIDLFNKYDEDGDGFILGSQAKTIFGTSGLPTKMLSHIWNLSDVSKDQKLDCQEFIMA 176

Query: 458 LYLMERYREGRPLPAVLPNNVMFDETLLSMTG 490
            +L+    +G  LP  LP +++     +S  G
Sbjct: 177 TFLIRSVLKGYELPNKLPESLITSSHYISSAG 208

BLAST of Lsi04G018190 vs. ExPASy Swiss-Prot
Match: Q9Z0R4 (Intersectin-1 OS=Mus musculus OX=10090 GN=Itsn1 PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 2.8e-08
Identity = 91/382 (23.82%), Postives = 155/382 (40.58%), Query Frame = 0

Query: 253 SLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS----GLNNAANN 312
           S  P ++  QP+    APA    G        A M  +T   P P  S    G++    +
Sbjct: 96  STLPPVMKQQPVAISSAPAFGIGGI-------ASMPPLTAVAPVPMGSIPVVGMSPPLVS 155

Query: 313 SIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKEVRATGPSP 372
           S+ P+A+ P+++ + P+ +P    +  +A + +   SS F  S    + N +++      
Sbjct: 156 SVPPAAVPPLANGAPPVIQPLPAFAHPAATLPK---SSSFSRSGPGSQLNTKLQKAQSFD 215

Query: 373 LISSGITTGARNSTSENAQFWPKMKSTDVQKYTKVFMEVDTDRDGRITGEQARNLFLSWR 432
           + S           +  A  W   +S+ + KY ++F   D    G +TG QAR + +   
Sbjct: 216 VAS-----------APPAAEWAVPQSSRL-KYRQLFNSHDKTMSGHLTGPQARTILMQSS 275

Query: 433 LPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVMFDETLLSMT 492
           LP+  L  +W+LSD D D  L+  EF  A++L++    G+PLP VLP   +        +
Sbjct: 276 LPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRS 335

Query: 493 GQSNIVHPNATWGPR-----PGFGQQQPQVTARSMAPTAGLRPPTPASKADGAKPSNEQK 552
           G    V  +++   R         +QQP+                P +  D  + + E+ 
Sbjct: 336 GSGMSVISSSSVDQRLPEEPSSEDEQQPE-------------KKLPVTFEDKKRENFERG 395

Query: 553 S-RAPVLEDSFLDQSEKGQQNSASLHAQDATANLFFSLLPYRRTVMQAVDLYDHYNVLHK 612
           S        + L+Q  K Q+  A L   +            R+  ++     +    L +
Sbjct: 396 SVELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELER 441

Query: 613 SRCDNRLNEITERASADKREAE 625
            R + R  EI ER  A KRE E
Sbjct: 456 QREEERRKEI-ERREAAKRELE 441

BLAST of Lsi04G018190 vs. ExPASy Swiss-Prot
Match: Q9HGL2 (Uncharacterized calcium-binding protein C800.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC800.10c PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.0e-07
Identity = 101/459 (22.00%), Postives = 193/459 (42.05%), Query Frame = 0

Query: 394 STDVQKYTKVFMEVDTDRDGRITGEQARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLRE 453
           S D+  + ++F  VD    G ++G +A + FL+ +LP +VL Q+WDLSD +++  L++ E
Sbjct: 257 SQDLTSFCQLFSNVDKAHKGYVSGSEAYSFFLASKLPEDVLAQIWDLSDTNSNGKLNIGE 316

Query: 454 FCFALYLMERYREGRPLPAVLPNNVMFDETLLSMTGQS-------------NIVHPNATW 513
           FC +LYL++    G+ LP VLP++++     L    +S             +  +PN T 
Sbjct: 317 FCISLYLIKLKLSGKELPKVLPSSMLSSVAPLMQKSKSVPTSIPSVVPANISSPNPNPTL 376

Query: 514 GPRPGFGQQQPQVT-------ARSMAPTAGLRPPTPASKA-DGAKPSNEQKSRAPVLEDS 573
            P P    +    T       A   +PT  L P   +S A   + P   + +  PV+   
Sbjct: 377 APNPTGPSRVTSGTEDLLSLDATPFSPT--LAPQHTSSNATKHSAPPVTKSAPFPVVSPL 436

Query: 574 FLDQSEKGQQNSASLHAQDATANLFFSLLPYRRTVMQAVDLYDHYNVLHKSRCDNRLNEI 633
            L+ +     + A+    ++  + FF    + +T+  A +  D  + +  S        I
Sbjct: 437 QLNHTPGFPTSPAA--KPNSPTSTFFPQSSFGQTI--AKNTMDKPSAVRTSVPSQLAAPI 496

Query: 634 TERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDVQERKTELHQAIIKMEQGG 693
            + ASA++ +  +   K E +  QV +    L   +   RDV    +++   + ++ +  
Sbjct: 497 PQVASAEQLKLAAEVPKLESQLSQVKKSNDDL---QKSSRDVAANLSDVKAKVSEIRKAY 556

Query: 694 SADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIELPVGWQPGIPDDAAIWDE 753
             +     +A +I  DI E  KA TE+  +  + +  A +  L    Q        + ++
Sbjct: 557 DEE---LAKAKQISLDI-ETNKAQTEQVNRE-YSILEATLNAL----QKQNKQKGEVLEQ 616

Query: 754 EWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTPDSSSNANGKMGNSLSTIN 813
                E E   N +      +   K +++D E+ LA  +   D  +     +      ++
Sbjct: 617 V--VAESEAAKNMVESSNASIQQLKSEVADKEQTLAQLHLQLDEMTQRLVSLDEESKAVS 676

Query: 814 -RGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFS 831
            R L+ E   ++S+ + A +          LE+   E S
Sbjct: 677 QRKLDLEYKINNSKTQLATATEEYHEHSKQLEAEKQELS 695

BLAST of Lsi04G018190 vs. ExPASy Swiss-Prot
Match: Q9WVE9 (Intersectin-1 OS=Rattus norvegicus OX=10116 GN=Itsn1 PE=1 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 6.8e-07
Identity = 87/358 (24.30%), Postives = 149/358 (41.62%), Query Frame = 0

Query: 279 SKSAFG----ADMFSVTPSPPRPESS----GLNNAANNSIGPSAIVPVSSVSQPLSKPNS 338
           S  AFG    A M  +T   P P  S    G++    +S+  +A+ P+++ + P+ +P  
Sbjct: 111 SAPAFGIGGMAGMPPLTAVAPVPMGSIPVVGMSPPLVSSVPQAAVPPLANGAPPVIQPLP 170

Query: 339 MESLQSAFVSRPLASSQFQLSQSSLEPNKEVRATGPSPLISSGITTGARNSTSENAQFWP 398
             +  +A + +   SS F  S    + N +++      + S           +  A  W 
Sbjct: 171 AFAHPAATLPK---SSSFSRSGPGSQLNTKLQKAQSFDVAS-----------APAAAEWA 230

Query: 399 KMKSTDVQKYTKVFMEVDTDRDGRITGEQARNLFLSWRLPREVLKQVWDLSDQDNDSMLS 458
             +S+ + KY ++F   D    G +TG QAR + +   LP+  L  +W+LSD D D  L+
Sbjct: 231 VPQSSRL-KYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLT 290

Query: 459 LREFCFALYLMERYREGRPLPAVLPNNVM--FDETLLSMTGQSNIVHPNATWG-PRPGFG 518
             EF  A++L++    G+PLP VLP   +      + S +G S I   +A    P     
Sbjct: 291 AEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSADQRLPEEPSS 350

Query: 519 QQQPQVTARSMAPTAGLRPPTPASKADGAKPSNEQKS-RAPVLEDSFLDQSEKGQQNSAS 578
           + + QV  +            P +  D  + + E+ +        + L+Q  K Q+  A 
Sbjct: 351 EDEQQVEKK-----------LPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQ 410

Query: 579 LHAQDATANLFFSLLPYRRTVMQAVDLYDHYNVLHKSRCDNRLNEITERASADKREAE 625
           L   +            R+  ++     +    L + R + R  EI ER  A KRE E
Sbjct: 411 LERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEI-ERREAAKRELE 441

BLAST of Lsi04G018190 vs. ExPASy TrEMBL
Match: A0A1S3BVY2 (epidermal growth factor receptor substrate 15-like 1 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103493829 PE=4 SV=1)

HSP 1 Score: 1771.1 bits (4586), Expect = 0.0e+00
Identity = 943/1073 (87.88%), Postives = 976/1073 (90.96%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAV+FFQGSNLPKN+LAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVSFFQGSNLPKNILAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQAVSAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAVSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGGGPAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGV PSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVGPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GLNNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLASSQFQLSQS LEP KE
Sbjct: 301  GLNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLASSQFQLSQSPLEPKKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRA GPSPLISSGITTGA NSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRAAGPSPLISSGITTGASNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVHPNA WGPRPGFGQQQPQVTARSM PTAGLRPPT  PAS+ADGAK
Sbjct: 481  FDETLLSMTGQSNIVHPNAAWGPRPGFGQQQPQVTARSMGPTAGLRPPTNIPASRADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQ EK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQPEKAQDAAASEKKVGETANVILDSKEKMEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKRE ESLGKKYEEKYKQVAEIASKLTIEEAKY DV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKRETESLGKKYEEKYKQVAEIASKLTIEEAKYHDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPK VSASKPK+SDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKSVSASKPKVSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S  NRGLESES+YSHSEDESARSPYGSPAAKTSLESPS +FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNNNRGLESESLYSHSEDESARSPYGSPAAKTSLESPSRDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y SFN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAYGSFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQREKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQEKFSRFDSISSSMDFGQSS 1020
            PQREKFSRFDSISSS DFG+NQEKFSRFDSISSS DFGHNQ+KFSRFDS+SSSMDFGQ+S
Sbjct: 961  PQREKFSRFDSISSSHDFGNNQEKFSRFDSISSSRDFGHNQDKFSRFDSMSSSMDFGQNS 1020

Query: 1021 QRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQSPKKSSDSWRAF 1067
            QRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSES SPKKSSD+WRAF
Sbjct: 1021 QRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESHSPKKSSDNWRAF 1030

BLAST of Lsi04G018190 vs. ExPASy TrEMBL
Match: A0A5A7V8H8 (Epidermal growth factor receptor substrate 15-like 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003770 PE=4 SV=1)

HSP 1 Score: 1761.5 bits (4561), Expect = 0.0e+00
Identity = 944/1092 (86.45%), Postives = 977/1092 (89.47%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAV+FFQGSNLPKN+LAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVSFFQGSNLPKNILAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQAVSAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAVSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGGGPAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGV PSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVGPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GLNNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLASSQFQLSQS LEP KE
Sbjct: 301  GLNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLASSQFQLSQSPLEPKKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRA GPSPLISSGITTGA NSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRAAGPSPLISSGITTGASNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVHPNA WGPRPGFGQQQPQVTARSM PTAGLRPPT  PAS+ADGAK
Sbjct: 481  FDETLLSMTGQSNIVHPNAAWGPRPGFGQQQPQVTARSMGPTAGLRPPTNIPASRADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQ EK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQPEKAQDAAASEKKVGETANVILDSKEKMEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKRE ESLGKKYEEKYKQVAEIASKLTIEEAKY DV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKRETESLGKKYEEKYKQVAEIASKLTIEEAKYHDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPK VSASKPK+SDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKSVSASKPKVSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S  NRGLESES+YSHSEDESARSPYGSPAAKTSLESPS +FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNNNRGLESESLYSHSEDESARSPYGSPAAKTSLESPSRDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y SFN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAYGSFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQR-------------------EKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQ 1020
            PQR                   EKFSRFDSISSSRDFG+NQEKFSRFDSISSS DFGHNQ
Sbjct: 961  PQREKFSRFDSISSSHDFGNNQEKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGHNQ 1020

Query: 1021 EKFSRFDSISSSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQ 1067
            +KFSRFDS+SSSMDFGQ+SQRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSES 
Sbjct: 1021 DKFSRFDSMSSSMDFGQNSQRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESH 1049

BLAST of Lsi04G018190 vs. ExPASy TrEMBL
Match: A0A1S3BV66 (epidermal growth factor receptor substrate 15-like 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493829 PE=4 SV=1)

HSP 1 Score: 1761.5 bits (4561), Expect = 0.0e+00
Identity = 944/1092 (86.45%), Postives = 977/1092 (89.47%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAV+FFQGSNLPKN+LAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVSFFQGSNLPKNILAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQAVSAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAVSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGGGPAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGV PSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVGPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GLNNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLASSQFQLSQS LEP KE
Sbjct: 301  GLNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLASSQFQLSQSPLEPKKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRA GPSPLISSGITTGA NSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRAAGPSPLISSGITTGASNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVHPNA WGPRPGFGQQQPQVTARSM PTAGLRPPT  PAS+ADGAK
Sbjct: 481  FDETLLSMTGQSNIVHPNAAWGPRPGFGQQQPQVTARSMGPTAGLRPPTNIPASRADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQ EK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQPEKAQDAAASEKKVGETANVILDSKEKMEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKRE ESLGKKYEEKYKQVAEIASKLTIEEAKY DV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKRETESLGKKYEEKYKQVAEIASKLTIEEAKYHDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPK VSASKPK+SDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKSVSASKPKVSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S  NRGLESES+YSHSEDESARSPYGSPAAKTSLESPS +FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNNNRGLESESLYSHSEDESARSPYGSPAAKTSLESPSRDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y SFN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAYGSFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQR-------------------EKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQ 1020
            PQR                   EKFSRFDSISSSRDFG+NQEKFSRFDSISSS DFGHNQ
Sbjct: 961  PQREKFSRFDSISSSHDFGNNQEKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGHNQ 1020

Query: 1021 EKFSRFDSISSSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQ 1067
            +KFSRFDS+SSSMDFGQ+SQRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSES 
Sbjct: 1021 DKFSRFDSMSSSMDFGQNSQRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESH 1049

BLAST of Lsi04G018190 vs. ExPASy TrEMBL
Match: A0A0A0L5X7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G664240 PE=4 SV=1)

HSP 1 Score: 1760.7 bits (4559), Expect = 0.0e+00
Identity = 943/1093 (86.28%), Postives = 980/1093 (89.66%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFD FF RADLDGDGRISGAEAV+FFQGSNLPKNVLAQ                    
Sbjct: 1    MDQFDLFFRRADLDGDGRISGAEAVSFFQGSNLPKNVLAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQA+SAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVG NQQYVSAQP
Sbjct: 121  KIPPPKIDLQALSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGANQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGG PAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGVPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGVSPS+PSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVSPSLPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            G NNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLA SQFQLSQS+ EPNKE
Sbjct: 301  GFNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLAGSQFQLSQSAPEPNKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRATGPSPLISSGITTGARNSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITG+Q
Sbjct: 361  VRATGPSPLISSGITTGARNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGDQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPA LPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAALPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSN+VHPNA W PRPGFGQQQPQVTARSMAPTAGLRPPT  PASKADGAK
Sbjct: 481  FDETLLSMTGQSNVVHPNAAWSPRPGFGQQQPQVTARSMAPTAGLRPPTNIPASKADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQSEK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQSEKAQDAAASEKKVGETANVILDSKEKIEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAK+RDV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKFRDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAII+MEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIRMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S INRGLE+ES+YSHSED SARSPYGSPAAKT LESPSH+FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNINRGLENESLYSHSEDGSARSPYGSPAAKTPLESPSHDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y SFN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAYGSFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQREKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQEKFSRFDSI---------- 1020
            PQREKFSRFDSISSSRDFG+NQEKFSRFDSISSS DFG+NQEKFSRFDSI          
Sbjct: 961  PQREKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGHNQ 1020

Query: 1021 ----------SSSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSES 1067
                      SSSMDFGQ+SQRHARFDSIGSS+DFGHGTFSFDDADPFGTSGPFKVSSES
Sbjct: 1021 DKFSRFDSMSSSSMDFGQNSQRHARFDSIGSSKDFGHGTFSFDDADPFGTSGPFKVSSES 1050

BLAST of Lsi04G018190 vs. ExPASy TrEMBL
Match: A0A5D3D8Y2 (Epidermal growth factor receptor substrate 15-like 1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00510 PE=4 SV=1)

HSP 1 Score: 1755.7 bits (4546), Expect = 0.0e+00
Identity = 943/1092 (86.36%), Postives = 976/1092 (89.38%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAV+FFQGSNLPKN+LAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVSFFQGSNLPKNILAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQAVSAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAVSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGGGPAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGV PSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVGPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GLNNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLASSQFQLSQS LEP KE
Sbjct: 301  GLNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLASSQFQLSQSPLEPKKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRA GPSPLISSGITTGA NSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRAAGPSPLISSGITTGASNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVHPNA WGPRPGFGQQQPQVTARSM PTAGLRPPT  PAS+ADGAK
Sbjct: 481  FDETLLSMTGQSNIVHPNAAWGPRPGFGQQQPQVTARSMGPTAGLRPPTNIPASRADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQ EK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQPEKAQDAAASEKKVGETANVILDSKEKMEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKRE ESLGKKYEEKYKQVAEIASKLTIEEAKY DV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKRETESLGKKYEEKYKQVAEIASKLTIEEAKYHDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPK VSASKPK+SDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKSVSASKPKVSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S  NRGLESES+YSHSEDESARSPYGSPAAKTSLESPS +FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNNNRGLESESLYSHSEDESARSPYGSPAAKTSLESPSRDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y  FN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAY-GFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQR-------------------EKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQ 1020
            PQR                   EKFSRFDSISSSRDFG+NQEKFSRFDSISSS DFGHNQ
Sbjct: 961  PQREKFSRFDSISSSHDFGNNQEKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGHNQ 1020

Query: 1021 EKFSRFDSISSSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQ 1067
            +KFSRFDS+SSSMDFGQ+SQRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSES 
Sbjct: 1021 DKFSRFDSMSSSMDFGQNSQRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESH 1048

BLAST of Lsi04G018190 vs. NCBI nr
Match: XP_038899165.1 (actin cytoskeleton-regulatory complex protein pan1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1822.4 bits (4719), Expect = 0.0e+00
Identity = 972/1082 (89.83%), Postives = 996/1082 (92.05%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAVAFFQGSNLPKNVLAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVAFFQGSNLPKNVLAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQA+SAPQSTSV AASPPQMS PAP GSQ+FGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAISAPQSTSVPAASPPQMSRPAPTGSQSFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT GGLPGGVASNTQ+VIASEPSGGGNVLGSNLSNPNDWLSGRPG GPAAGP
Sbjct: 181  NPSMRLPQATLGGLPGGVASNTQMVIASEPSGGGNVLGSNLSNPNDWLSGRPGSGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGVSPSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SK AFGADMFSVTPSPPRPESS
Sbjct: 241  RGVSPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKPAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GL NAANNSIGP+AIVPVSSVSQPLSKPNSMESLQSAF+SRPLA SQFQLSQSSLEPNK+
Sbjct: 301  GLTNAANNSIGPAAIVPVSSVSQPLSKPNSMESLQSAFISRPLAGSQFQLSQSSLEPNKD 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRATGPSPL+SSGITTGARNSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRATGPSPLVSSGITTGARNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPRE+LKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREILKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVH NATWGPRPGFGQQQPQVTARSMAPTAGLRPPT  PASKADGAK
Sbjct: 481  FDETLLSMTGQSNIVHSNATWGPRPGFGQQQPQVTARSMAPTAGLRPPTNVPASKADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDA---------TANLFF---SLLPYRR 600
             SN+QKSRAPVL+DSFLDQSEKGQQNS +L+AQDA         TAN+       + Y R
Sbjct: 541  LSNDQKSRAPVLDDSFLDQSEKGQQNSVNLNAQDAIASEKKVGETANVILDSKEKIEYYR 600

Query: 601  TVMQAVDLYDHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLT 660
            T+MQ +       VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLT
Sbjct: 601  TMMQEL-------VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLT 660

Query: 661  IEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGF 720
            IEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGF
Sbjct: 661  IEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGF 720

Query: 721  DVKSAAIIELPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEK 780
            DVKSAAIIELPVGWQPGIPDDAA WDEEWDKFEDEGFSNDLNLDPKGV+ASKPKMSDSEK
Sbjct: 721  DVKSAAIIELPVGWQPGIPDDAATWDEEWDKFEDEGFSNDLNLDPKGVTASKPKMSDSEK 780

Query: 781  DLADYNSTPDSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESP 840
            DLADYNSTPDSSSNANGK GN LST NRGLESESVYSHSEDESARSPYGSPAAKTSLESP
Sbjct: 781  DLADYNSTPDSSSNANGKTGNYLSTNNRGLESESVYSHSEDESARSPYGSPAAKTSLESP 840

Query: 841  SHEFSDAGYEKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-AS 900
            SHEFSDAGYEKSPE+YRSFNES W GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF AS
Sbjct: 841  SHEFSDAGYEKSPEAYRSFNESTW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGAS 900

Query: 901  DFDTSSVKTGSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDS 960
            DFDTSSVKTGSP  DSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVG+HFFDNSSRFDS
Sbjct: 901  DFDTSSVKTGSP--DSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGEHFFDNSSRFDS 960

Query: 961  SSMQDGSFSPQREKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQEKFSRFDSIS 1020
             SMQDGSFSPQREKFSRFDSISSSRDFGHNQEKFSRFDS+SSS DFGHNQ+KFSRFDSIS
Sbjct: 961  FSMQDGSFSPQREKFSRFDSISSSRDFGHNQEKFSRFDSMSSSRDFGHNQDKFSRFDSIS 1020

Query: 1021 SSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQSPKKSSDSWR 1067
            SSMD+GQSSQRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSESQSPKKSSDSWR
Sbjct: 1021 SSMDYGQSSQRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESQSPKKSSDSWR 1041

BLAST of Lsi04G018190 vs. NCBI nr
Match: XP_038899166.1 (actin cytoskeleton-regulatory complex protein pan1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1815.8 bits (4702), Expect = 0.0e+00
Identity = 971/1082 (89.74%), Postives = 995/1082 (91.96%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAVAFFQGSNLPKNVLAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVAFFQGSNLPKNVLAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQA+SAPQSTSV AASPPQMS PAP GSQ+FGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAISAPQSTSVPAASPPQMSRPAPTGSQSFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT GGLPGGVASNTQ+VIASEPSGGGNVLGSNLSNPNDWLSGRPG GPAAGP
Sbjct: 181  NPSMRLPQATLGGLPGGVASNTQMVIASEPSGGGNVLGSNLSNPNDWLSGRPGSGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGVSPSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SK AFGADMFSVTPSPPRPESS
Sbjct: 241  RGVSPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKPAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GL NAANNSIGP+AIVPVSSVSQPLSKPNSMESLQSAF+SRPLA SQFQLSQSSLEPNK+
Sbjct: 301  GLTNAANNSIGPAAIVPVSSVSQPLSKPNSMESLQSAFISRPLAGSQFQLSQSSLEPNKD 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRATGPSPL+SSGITTGARNSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRATGPSPLVSSGITTGARNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPRE+LKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREILKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVH NATWGPRPGFGQQQPQVTARSMAPTAGLRPPT  PASKADGAK
Sbjct: 481  FDETLLSMTGQSNIVHSNATWGPRPGFGQQQPQVTARSMAPTAGLRPPTNVPASKADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDA---------TANLFF---SLLPYRR 600
             SN+QKSRAPVL+DSFLDQSEKGQQNS +L+AQDA         TAN+       + Y R
Sbjct: 541  LSNDQKSRAPVLDDSFLDQSEKGQQNSVNLNAQDAIASEKKVGETANVILDSKEKIEYYR 600

Query: 601  TVMQAVDLYDHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLT 660
            T+MQ +       VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLT
Sbjct: 601  TMMQEL-------VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLT 660

Query: 661  IEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGF 720
            IEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGF
Sbjct: 661  IEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGF 720

Query: 721  DVKSAAIIELPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEK 780
            DVKSAAIIELPVGWQPGIPDDAA WDEEWDKFEDEGFSNDLNLDPKGV+ASKPKMSDSEK
Sbjct: 721  DVKSAAIIELPVGWQPGIPDDAATWDEEWDKFEDEGFSNDLNLDPKGVTASKPKMSDSEK 780

Query: 781  DLADYNSTPDSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESP 840
            DLADYNSTPDSSSNANGK GN LST NRGLESESVYSHSEDESARSPYGSPAAKTSLESP
Sbjct: 781  DLADYNSTPDSSSNANGKTGNYLSTNNRGLESESVYSHSEDESARSPYGSPAAKTSLESP 840

Query: 841  SHEFSDAGYEKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-AS 900
            SHEFSDAGYEKSPE+Y SFNES W GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF AS
Sbjct: 841  SHEFSDAGYEKSPEAY-SFNESTW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGAS 900

Query: 901  DFDTSSVKTGSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDS 960
            DFDTSSVKTGSP  DSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVG+HFFDNSSRFDS
Sbjct: 901  DFDTSSVKTGSP--DSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGEHFFDNSSRFDS 960

Query: 961  SSMQDGSFSPQREKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQEKFSRFDSIS 1020
             SMQDGSFSPQREKFSRFDSISSSRDFGHNQEKFSRFDS+SSS DFGHNQ+KFSRFDSIS
Sbjct: 961  FSMQDGSFSPQREKFSRFDSISSSRDFGHNQEKFSRFDSMSSSRDFGHNQDKFSRFDSIS 1020

Query: 1021 SSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQSPKKSSDSWR 1067
            SSMD+GQSSQRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSESQSPKKSSDSWR
Sbjct: 1021 SSMDYGQSSQRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESQSPKKSSDSWR 1040

BLAST of Lsi04G018190 vs. NCBI nr
Match: XP_008452993.1 (PREDICTED: epidermal growth factor receptor substrate 15-like 1 isoform X3 [Cucumis melo])

HSP 1 Score: 1771.1 bits (4586), Expect = 0.0e+00
Identity = 943/1073 (87.88%), Postives = 976/1073 (90.96%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAV+FFQGSNLPKN+LAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVSFFQGSNLPKNILAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQAVSAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAVSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGGGPAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGV PSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVGPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GLNNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLASSQFQLSQS LEP KE
Sbjct: 301  GLNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLASSQFQLSQSPLEPKKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRA GPSPLISSGITTGA NSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRAAGPSPLISSGITTGASNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVHPNA WGPRPGFGQQQPQVTARSM PTAGLRPPT  PAS+ADGAK
Sbjct: 481  FDETLLSMTGQSNIVHPNAAWGPRPGFGQQQPQVTARSMGPTAGLRPPTNIPASRADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQ EK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQPEKAQDAAASEKKVGETANVILDSKEKMEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKRE ESLGKKYEEKYKQVAEIASKLTIEEAKY DV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKRETESLGKKYEEKYKQVAEIASKLTIEEAKYHDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPK VSASKPK+SDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKSVSASKPKVSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S  NRGLESES+YSHSEDESARSPYGSPAAKTSLESPS +FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNNNRGLESESLYSHSEDESARSPYGSPAAKTSLESPSRDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y SFN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAYGSFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQREKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQEKFSRFDSISSSMDFGQSS 1020
            PQREKFSRFDSISSS DFG+NQEKFSRFDSISSS DFGHNQ+KFSRFDS+SSSMDFGQ+S
Sbjct: 961  PQREKFSRFDSISSSHDFGNNQEKFSRFDSISSSRDFGHNQDKFSRFDSMSSSMDFGQNS 1020

Query: 1021 QRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQSPKKSSDSWRAF 1067
            QRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSES SPKKSSD+WRAF
Sbjct: 1021 QRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESHSPKKSSDNWRAF 1030

BLAST of Lsi04G018190 vs. NCBI nr
Match: XP_008452991.1 (PREDICTED: epidermal growth factor receptor substrate 15-like 1 isoform X1 [Cucumis melo] >KAA0064652.1 epidermal growth factor receptor substrate 15-like 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1761.5 bits (4561), Expect = 0.0e+00
Identity = 944/1092 (86.45%), Postives = 977/1092 (89.47%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFDAFF RADLDGDGRISGAEAV+FFQGSNLPKN+LAQ                    
Sbjct: 1    MDQFDAFFRRADLDGDGRISGAEAVSFFQGSNLPKNILAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQAVSAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVGVNQQYVSAQP
Sbjct: 121  KIPPPKIDLQAVSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGVNQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGGGPAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGGPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGV PSVPSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVGPSVPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            GLNNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLASSQFQLSQS LEP KE
Sbjct: 301  GLNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLASSQFQLSQSPLEPKKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRA GPSPLISSGITTGA NSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITGEQ
Sbjct: 361  VRAAGPSPLISSGITTGASNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGEQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPAVLPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAVLPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSNIVHPNA WGPRPGFGQQQPQVTARSM PTAGLRPPT  PAS+ADGAK
Sbjct: 481  FDETLLSMTGQSNIVHPNAAWGPRPGFGQQQPQVTARSMGPTAGLRPPTNIPASRADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQ EK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQPEKAQDAAASEKKVGETANVILDSKEKMEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKRE ESLGKKYEEKYKQVAEIASKLTIEEAKY DV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKRETESLGKKYEEKYKQVAEIASKLTIEEAKYHDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPK VSASKPK+SDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKSVSASKPKVSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S  NRGLESES+YSHSEDESARSPYGSPAAKTSLESPS +FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNNNRGLESESLYSHSEDESARSPYGSPAAKTSLESPSRDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y SFN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAYGSFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQR-------------------EKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQ 1020
            PQR                   EKFSRFDSISSSRDFG+NQEKFSRFDSISSS DFGHNQ
Sbjct: 961  PQREKFSRFDSISSSHDFGNNQEKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGHNQ 1020

Query: 1021 EKFSRFDSISSSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSESQ 1067
            +KFSRFDS+SSSMDFGQ+SQRHARFDSIGSS+DFGHG FSFDDADPFGTSGPFKVSSES 
Sbjct: 1021 DKFSRFDSMSSSMDFGQNSQRHARFDSIGSSKDFGHGAFSFDDADPFGTSGPFKVSSESH 1049

BLAST of Lsi04G018190 vs. NCBI nr
Match: XP_011654279.1 (epidermal growth factor receptor substrate 15-like 1 isoform X1 [Cucumis sativus] >KGN55516.1 hypothetical protein Csa_012693 [Cucumis sativus])

HSP 1 Score: 1760.7 bits (4559), Expect = 0.0e+00
Identity = 943/1093 (86.28%), Postives = 980/1093 (89.66%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQFD FF RADLDGDGRISGAEAV+FFQGSNLPKNVLAQ                    
Sbjct: 1    MDQFDLFFRRADLDGDGRISGAEAVSFFQGSNLPKNVLAQ-------------------- 60

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA
Sbjct: 61   -----------IWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKIDLQA+SAPQSTSV AASPPQMSIPAP GSQNFGFRGQGVPNVG NQQYVSAQP
Sbjct: 121  KIPPPKIDLQALSAPQSTSVPAASPPQMSIPAPTGSQNFGFRGQGVPNVGANQQYVSAQP 180

Query: 181  NPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNVLGSNLSNPNDWLSGRPGGGPAAGP 240
            NPSMRLPQAT    PGGVASN QLV++SEPSGGGN+LGSNLSNPNDWL+GRPGG PAAGP
Sbjct: 181  NPSMRLPQAT----PGGVASNMQLVVSSEPSGGGNLLGSNLSNPNDWLNGRPGGVPAAGP 240

Query: 241  RGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGFVSKSAFGADMFSVTPSPPRPESS 300
            RGVSPS+PSPATSLSPAL+TSQPMPNDRAPAVTGNGF SKSAFGADMFSVTPSPPRPESS
Sbjct: 241  RGVSPSLPSPATSLSPALMTSQPMPNDRAPAVTGNGFASKSAFGADMFSVTPSPPRPESS 300

Query: 301  GLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKE 360
            G NNAAN+SIGPSAIVPVSSVSQPLSK  S+ESLQSAFVSRPLA SQFQLSQS+ EPNKE
Sbjct: 301  GFNNAANSSIGPSAIVPVSSVSQPLSKSTSLESLQSAFVSRPLAGSQFQLSQSAPEPNKE 360

Query: 361  VRATGPSPLISSGITTGARNSTSENAQF-WPKMKSTDVQKYTKVFMEVDTDRDGRITGEQ 420
            VRATGPSPLISSGITTGARNSTSENAQF WPKMK TDVQKYTKVFMEVDTDRDGRITG+Q
Sbjct: 361  VRATGPSPLISSGITTGARNSTSENAQFTWPKMKPTDVQKYTKVFMEVDTDRDGRITGDQ 420

Query: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRPLPAVLPNNVM 480
            ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSL+EFCFALYLMERYREGRPLPA LPNNVM
Sbjct: 421  ARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLKEFCFALYLMERYREGRPLPAALPNNVM 480

Query: 481  FDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRPPT--PASKADGAK 540
            FDETLLSMTGQSN+VHPNA W PRPGFGQQQPQVTARSMAPTAGLRPPT  PASKADGAK
Sbjct: 481  FDETLLSMTGQSNVVHPNAAWSPRPGFGQQQPQVTARSMAPTAGLRPPTNIPASKADGAK 540

Query: 541  PSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFF---SLLPYRRTVMQAVDLY 600
             SNEQKSRAPVLEDSFLDQSEK Q  +AS      TAN+       + Y RT+MQ +   
Sbjct: 541  LSNEQKSRAPVLEDSFLDQSEKAQDAAASEKKVGETANVILDSKEKIEYYRTMMQEL--- 600

Query: 601  DHYNVLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDV 660
                VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAK+RDV
Sbjct: 601  ----VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKFRDV 660

Query: 661  QERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720
            QERKTELHQAII+MEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE
Sbjct: 661  QERKTELHQAIIRMEQGGSADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIE 720

Query: 721  LPVGWQPGIPDDAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780
            LPVGWQPGIPD+AAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP
Sbjct: 721  LPVGWQPGIPDNAAIWDEEWDKFEDEGFSNDLNLDPKGVSASKPKMSDSEKDLADYNSTP 780

Query: 781  DSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGSPAAKTSLESPSHEFSDAGY 840
            DSSSNANGK G+S S INRGLE+ES+YSHSED SARSPYGSPAAKT LESPSH+FSDAG+
Sbjct: 781  DSSSNANGKTGHSFSNINRGLENESLYSHSEDGSARSPYGSPAAKTPLESPSHDFSDAGF 840

Query: 841  EKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF-ASDFDTSSVKT 900
            EKSPE+Y SFN+SAW GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFF +SDFDTSSV+T
Sbjct: 841  EKSPEAYGSFNDSAW-GTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFGSSDFDTSSVRT 900

Query: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHFFDNSSRFDSSSMQDGSFS 960
            GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDH+FDNSSRFDS SMQDGSFS
Sbjct: 901  GSPNADSFFQRKSPFFEDSVPPTPLSRFGNSSPRYSDVGDHYFDNSSRFDSFSMQDGSFS 960

Query: 961  PQREKFSRFDSISSSRDFGHNQEKFSRFDSISSSHDFGHNQEKFSRFDSI---------- 1020
            PQREKFSRFDSISSSRDFG+NQEKFSRFDSISSS DFG+NQEKFSRFDSI          
Sbjct: 961  PQREKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGNNQEKFSRFDSISSSRDFGHNQ 1020

Query: 1021 ----------SSSMDFGQSSQRHARFDSIGSSRDFGHGTFSFDDADPFGTSGPFKVSSES 1067
                      SSSMDFGQ+SQRHARFDSIGSS+DFGHGTFSFDDADPFGTSGPFKVSSES
Sbjct: 1021 DKFSRFDSMSSSSMDFGQNSQRHARFDSIGSSKDFGHGTFSFDDADPFGTSGPFKVSSES 1050

BLAST of Lsi04G018190 vs. TAIR 10
Match: AT1G20760.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 810.4 bits (2092), Expect = 1.7e-234
Identity = 546/1115 (48.97%), Postives = 681/1115 (61.08%), Query Frame = 0

Query: 1    MDQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSV 60
            MDQF+A+F RADLDGDGRISGAEAV FFQGS L K VLAQ                    
Sbjct: 8    MDQFEAYFKRADLDGDGRISGAEAVGFFQGSGLSKQVLAQ-------------------- 67

Query: 61   IKFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAA 120
                       IW  +D+  +GFL R  FYN+LRLVTVAQSKR+LTPEIV AAL  PAAA
Sbjct: 68   -----------IWSLSDRSHSGFLDRQNFYNSLRLVTVAQSKRDLTPEIVNAALNTPAAA 127

Query: 121  KIPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVGVNQQYVSAQP 180
            KIPPPKI+L A+ AP+         P  +   P+     GF G G PN  VNQ Y   Q 
Sbjct: 128  KIPPPKINLSAIPAPRPN-------PAATTVGPVS----GFGGPGAPNAIVNQNYFPPQQ 187

Query: 181  NPSMRL---------------PQATPGGLPG-------GVASNTQLVIASEPSGGGNVLG 240
            N  MR                P+  P  L G       G  ++    + +  SG     G
Sbjct: 188  NQQMRPNQGISGLTSLRPAAGPEYRPSALSGQFQPVPVGSVTHPPQPVPTSVSGP----G 247

Query: 241  SNLSNPNDWLSGRPG-GGPAAGPRGVSPSVPSPATSLSPALVTSQPMPNDRAPAVTGNGF 300
            S+  N N   +G     G ++G  G S + PSP        +  +   + +A  V+GN  
Sbjct: 248  SSTFNLNSLYAGAGNTSGYSSGFGGGSLAAPSPG-------LKQESHIDPKALVVSGN-- 307

Query: 301  VSKSAFGADMFSVTPSPPRPESSGLNNAANNSIGPSAIVPVSSVSQPLSKPNSMESLQSA 360
                  G DMFS       P  S      N+SI  SAIVP S+  QP +KPN+++SLQS 
Sbjct: 308  ------GGDMFSSFQQKQEPTLS------NSSIS-SAIVPTSAGIQPPTKPNALDSLQST 367

Query: 361  FVSRPLASSQFQLSQSSLEPNKEVRATGPSPLISSGITTGARNST--SENAQFWPKMKST 420
            F   P + +Q Q  + +      V + GPS  +  G   G+ +ST    N   WPKMK +
Sbjct: 368  FSMLP-SGNQLQQPRPAASSQPAVSSQGPSSGLPPGSAVGSGHSTPAGNNQPPWPKMKPS 427

Query: 421  DVQKYTKVFMEVDTDRDGRITGEQARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFC 480
            DVQKYTKVFMEVD+D+DG+ITGEQARNLFLSWRLPREVLK VW+LSDQDND+MLSLREFC
Sbjct: 428  DVQKYTKVFMEVDSDKDGKITGEQARNLFLSWRLPREVLKHVWELSDQDNDTMLSLREFC 487

Query: 481  FALYLMERYREGRPLPAVLPNNVMFDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTA 540
             +LYLMERYREGRPLP  LP+++MFDETLLS++G  +  + NA WG   GF  QQP + A
Sbjct: 488  ISLYLMERYREGRPLPTALPSSIMFDETLLSISGAPSHGYANAGWGSGQGF-VQQPGMGA 547

Query: 541  RSMAPTAGLRPPTPA--SKADGAKPSNEQKSRAPVLEDSFLDQSEKGQQNSASL------ 600
            R + PT G+RPP PA   +     P N+ +++APVL+  F +    G   S++L      
Sbjct: 548  RPITPTTGMRPPVPAPGPQPGSGIPPNQPRNQAPVLDAPFANHLGNGYSASSNLPEAAAD 607

Query: 601  -HAQDATANLFF---SLLPYRRTVMQAVDLYDHYNVLHKSRCDNRLNEITERASADKREA 660
                D   N +      L Y RT MQ +       VL+KSRCDNRLNEI+ERASADKREA
Sbjct: 608  EEKVDEKQNAYMDSREKLDYYRTKMQDI-------VLYKSRCDNRLNEISERASADKREA 667

Query: 661  ESLGKKYEEKYKQVAEIASKLTIEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRAD 720
            E+L KKYEEKYKQVAEI SKLTIEEA++R+++ RK EL QAI+ MEQGGSADG+LQVRAD
Sbjct: 668  ETLAKKYEEKYKQVAEIGSKLTIEEARFREIEGRKMELSQAIVNMEQGGSADGLLQVRAD 727

Query: 721  RIQSDIEELIKALTERCKKHGFDVKSAAIIELPVGWQPGIPDDAAIWDEEWDKFEDEGFS 780
            RIQSD+EEL+KALTERCKKHG +V S A+++LP GWQPGI + AA+WDEEWDKFEDEGF 
Sbjct: 728  RIQSDLEELMKALTERCKKHGLEVNSKALVDLPAGWQPGIQEGAALWDEEWDKFEDEGFG 787

Query: 781  NDLNLDPKGVSASKPKMSDSEKD---LADYNSTPDSSSNANGKMGNSLSTINRGLESESV 840
            N++  D      SK + S  EK+   + D +  PDS ++     G    T +R  ESE  
Sbjct: 788  NEITFD-----KSKEQNSSGEKENGTVDDGSGPPDSPTHVEDNYGPFSETSDRHHESEYG 847

Query: 841  YSHSEDESARSPYGSPAAKTSLESPSHEFSDAGYEKSPESYRSFNESAWGGTFDNNDDVD 900
             +HSED   RSP  SP ++ + E PS ++S        ++   F++S W   FD NDDVD
Sbjct: 848  TTHSED---RSPRDSPVSRNATEVPSPDYSQG------KNSEFFDDSNWASAFDTNDDVD 907

Query: 901  SVWGIKPVNTKEPDSEKHRDFFAS--DFDTSSVKTGSPNADSF-FQRKSPF-FEDSVPPT 960
            SVWG       +    +  D+F S  DF  ++ +  SP++ SF  QRKS F F+DSVP T
Sbjct: 908  SVWGF------DASKSQDGDYFGSGDDFGGNTARADSPSSRSFGAQRKSQFAFDDSVPST 967

Query: 961  PLSRFGNSSPRYSDVG--DHFFDNSSRFDS--SSMQDGSFSPQREKFSRFDSISSSRDFG 1020
            PLSRFGNS PR+SD    D+ FD+ SRFDS  +S     FS Q E+ SRFDSI+SS+DFG
Sbjct: 968  PLSRFGNSPPRFSDASARDNNFDSFSRFDSFNTSEAGAGFSSQPERLSRFDSINSSKDFG 1019

Query: 1021 HNQEKFSRFDSISSSHDFGHNQEKFSRFDSISSSMDFGQSSQRHARFDSIGSSRDF-GHG 1067
                 FSRFDSI+SS D     EKFSRFDSI+SS DFG  S   +RFDS+ S++DF G  
Sbjct: 1028 --GAAFSRFDSINSSRDV-TGAEKFSRFDSINSSKDFGGPSL--SRFDSMNSTKDFSGSH 1019

BLAST of Lsi04G018190 vs. TAIR 10
Match: AT1G21630.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 580.5 bits (1495), Expect = 2.8e-165
Identity = 472/1268 (37.22%), Postives = 626/1268 (49.37%), Query Frame = 0

Query: 2    DQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSVI 61
            D FD +F RADLDGDG ISGAEAVAFFQGSNLPK+VLAQV                    
Sbjct: 11   DLFDTYFRRADLDGDGHISGAEAVAFFQGSNLPKHVLAQV-------------------- 70

Query: 62   KFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAAK 121
                       W +AD +K G+LGR EFYNAL+LVTVAQS+RELT EIVKAA+Y PA+A 
Sbjct: 71   -----------WSYADSKKAGYLGRAEFYNALKLVTVAQSRRELTAEIVKAAIYSPASAN 130

Query: 122  IPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVG-----VNQQYV 181
            IP PKI+L A  +PQ   V  A+  Q     P  S   G RG   P++G      NQQ V
Sbjct: 131  IPAPKINLAATPSPQPRGVLPATQAQGVTSMP--SVAAGVRG---PHMGGTVSTSNQQVV 190

Query: 182  SAQPNPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNV-LGSNLSNPNDWLSGRPGGG 241
              Q N    +P       P     N Q      P+GG N    +N   P+DWLSGR    
Sbjct: 191  PGQQNQFTGIP-------PSQTQQNFQ--SPGMPAGGTNAPRPANQPMPSDWLSGR---- 250

Query: 242  PAAGPRG-VSPSVPSPATS-----------------LSPALVTS-QPMPNDRAP------ 301
             + GP G V+  +PS  ++                 ++PA+ +S    P + AP      
Sbjct: 251  -SVGPSGNVNSQIPSSQSTYGLTAPNSTANHITKPHITPAVTSSTTTRPQESAPVHNPQE 310

Query: 302  -------------------------AVTGNGFVSKSAFGADMFSVTPSPPRPESSGLNNA 361
                                     A +GNGF S S FG D+FSVT + P+   +G  + 
Sbjct: 311  SSATFGSRVSNVPSNQLVPKDPKELAASGNGFTSDSLFG-DVFSVTSTQPKQHPTGSAST 370

Query: 362  ANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKEVRATG 421
               S   +  V    ++Q + + +S+   Q   +S+     Q QL+ +S +P     A  
Sbjct: 371  TGISSVTTGTVAGPEITQSVVRQSSIP--QQGSLSQHAVGVQTQLTGNSGQPYTSSGAAS 430

Query: 422  PSPLISSGITTGARNSTSENAQF----------------WPKMKSTDVQKYTKVFMEVDT 481
              P  + G+   A +  ++                    WPKM   DVQKYTKVF++VDT
Sbjct: 431  GPPGSTVGVGISATSQLAQRPPHPHSQPQPRPQGQSQPPWPKMTPADVQKYTKVFVQVDT 490

Query: 482  DRDGRITGEQARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRP 541
            DRDG+ITG QARNLFLSWRLPR+ LKQVWDLSDQDNDSMLSLREFC A+YLMERYREGRP
Sbjct: 491  DRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSLREFCIAVYLMERYREGRP 550

Query: 542  LPAVLPNNVMFDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRP-PT 601
            LP V P++++  E++ +  GQS   H NA+WG   GF QQ      R  A   G  P P 
Sbjct: 551  LPPVFPSSIIHSESMFTSPGQSVAPHGNASWGHPQGFQQQPHPGGLRPPAGPKGKPPRPV 610

Query: 602  PASKADGAKPSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATANLFFSLLPYRRTVM 661
            P S +DG     + K + PVLE   +DQ  K +Q+S +   ++ATA +   +    + + 
Sbjct: 611  PLSPSDGMVQPTQPKRKMPVLEKPLVDQLSKEEQDSLNTKFEEATA-VDKKVDELEKEIA 670

Query: 662  QAVDLYDHYN------VLHKSRCDNRLNEITERASADKREAESLGKKYEEKYKQVAEIAS 721
             +    D +       VL+KSRCDNR NEI ER   DKRE ESL KKYEEKYK+   + S
Sbjct: 671  DSKQKIDFFRAKMQELVLYKSRCDNRYNEIAERVLGDKRELESLAKKYEEKYKKSGNVGS 730

Query: 722  KLTIEEAKYRDVQERKTELHQAIIKMEQGGSADGILQVRADRIQSDIEELIKALTERCKK 781
            KLTIEEA +RD+QE+K EL+QAI+K E+G   D I++ R + IQS +EELIK L ERCK+
Sbjct: 731  KLTIEEATFRDIQEKKMELYQAIVKFEEGKLDDSIVKERTEHIQSGLEELIKNLNERCKQ 790

Query: 782  HGFDVKSAAIIELPVGWQPGIPDDAAIWDEEWDKFEDEGFS--NDLNLDPKGVSASKPKM 841
            +G   K  +++ELP GWQPGI + AA WDE+WDK EDEGF+   +L LD + V A   + 
Sbjct: 791  YGVRGKPTSLVELPFGWQPGIQEGAADWDEDWDKLEDEGFTFVKELTLDIQNVIAPPKEK 850

Query: 842  SDS---EKDLADYNSTPDSSSNANGKMGNSLSTINRGLESESVYSHSEDESARSPYGS-- 901
            S +   E D++       S S+A+ K G   S+     +SE     + D  AR   GS  
Sbjct: 851  SSAWRKEVDVSSKEGEDVSFSDADSKTGKKQSSGEE--DSEQSEGKTSDVDARDKNGSLD 910

Query: 902  -PAAKTSLESPSH-EFSDAGYEKSPESYRSFNESAWGGTFDNNDDVDSVWGIKPVNTKEP 961
                +  +E+ S     D   E   +   S   +     +D++D+ DSV  + P N K+ 
Sbjct: 911  DSKVRKGIEADSSPRTKDTRSENGHDDGESTASAGKTVNYDSHDETDSVSSVNPDNGKDK 970

Query: 962  DSEKHRDFFA-----SDFDTSSVKTGSPNADSFFQRKSPFFEDSVP----------PTPL 1021
            D  K+   F       DF    +KTGS  ++ F   K   F DSVP          PT  
Sbjct: 971  DHGKYDSGFGFGFGFDDFSIKPIKTGSTISNDFLPPKLSIFADSVPSPPANASDVSPTKP 1030

Query: 1022 SRFGNSSPRYSDVGD-----------------------------HFFDNS---------- 1067
            S F +S P      +                              FFD+S          
Sbjct: 1031 SLFADSVPSTPATNNASYPGQKSFFDDSVPSTPAYPGNLFAEKKSFFDDSVPSTPAYPGN 1090

BLAST of Lsi04G018190 vs. TAIR 10
Match: AT1G21630.2 (Calcium-binding EF hand family protein )

HSP 1 Score: 571.6 bits (1472), Expect = 1.3e-162
Identity = 479/1297 (36.93%), Postives = 631/1297 (48.65%), Query Frame = 0

Query: 2    DQFDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSVI 61
            D FD +F RADLDGDG ISGAEAVAFFQGSNLPK+VLAQV                    
Sbjct: 11   DLFDTYFRRADLDGDGHISGAEAVAFFQGSNLPKHVLAQV-------------------- 70

Query: 62   KFEATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAALYGPAAAK 121
                       W +AD +K G+LGR EFYNAL+LVTVAQS+RELT EIVKAA+Y PA+A 
Sbjct: 71   -----------WSYADSKKAGYLGRAEFYNALKLVTVAQSRRELTAEIVKAAIYSPASAN 130

Query: 122  IPPPKIDLQAVSAPQSTSVSAASPPQMSIPAPMGSQNFGFRGQGVPNVG-----VNQQYV 181
            IP PKI+L A  +PQ   V  A+  Q     P  S   G RG   P++G      NQQ V
Sbjct: 131  IPAPKINLAATPSPQPRGVLPATQAQGVTSMP--SVAAGVRG---PHMGGTVSTSNQQVV 190

Query: 182  SAQPNPSMRLPQATPGGLPGGVASNTQLVIASEPSGGGNV-LGSNLSNPNDWLSGRPGGG 241
              Q N    +P       P     N Q      P+GG N    +N   P+DWLSGR    
Sbjct: 191  PGQQNQFTGIP-------PSQTQQNFQ--SPGMPAGGTNAPRPANQPMPSDWLSGR---- 250

Query: 242  PAAGPRG-VSPSVPSPATS-----------------LSPALVTS-QPMPNDRAP------ 301
             + GP G V+  +PS  ++                 ++PA+ +S    P + AP      
Sbjct: 251  -SVGPSGNVNSQIPSSQSTYGLTAPNSTANHITKPHITPAVTSSTTTRPQESAPVHNPQE 310

Query: 302  -------------------------AVTGNGFVSKSAFGADMFSVTPSPPRPESSGLNNA 361
                                     A +GNGF S S FG D+FSVT + P+   +G  + 
Sbjct: 311  SSATFGSRVSNVPSNQLVPKDPKELAASGNGFTSDSLFG-DVFSVTSTQPKQHPTGSAST 370

Query: 362  ANNSIGPSAIVPVSSVSQPLSKPNSMESLQSAFVSRPLASSQFQLSQSSLEPNKEVRATG 421
               S   +  V    ++Q + + +S+   Q   +S+     Q QL+ +S +P     A  
Sbjct: 371  TGISSVTTGTVAGPEITQSVVRQSSIP--QQGSLSQHAVGVQTQLTGNSGQPYTSSGAAS 430

Query: 422  PSPLISSGITTGARNSTSENAQF----------------WPKMKSTDVQKYTKVFMEVDT 481
              P  + G+   A +  ++                    WPKM   DVQKYTKVF++VDT
Sbjct: 431  GPPGSTVGVGISATSQLAQRPPHPHSQPQPRPQGQSQPPWPKMTPADVQKYTKVFVQVDT 490

Query: 482  DRDGRITGEQARNLFLSWRLPREVLKQVWDLSDQDNDSMLSLREFCFALYLMERYREGRP 541
            DRDG+ITG QARNLFLSWRLPR+ LKQVWDLSDQDNDSMLSLREFC A+YLMERYREGRP
Sbjct: 491  DRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSLREFCIAVYLMERYREGRP 550

Query: 542  LPAVLPNNVMFDETLLSMTGQSNIVHPNATWGPRPGFGQQQPQVTARSMAPTAGLRP-PT 601
            LP V P++++  E++ +  GQS   H NA+WG   GF QQ      R  A   G  P P 
Sbjct: 551  LPPVFPSSIIHSESMFTSPGQSVAPHGNASWGHPQGFQQQPHPGGLRPPAGPKGKPPRPV 610

Query: 602  PASKADGAKPSNEQKSRAPVLEDSFLDQSEKGQQNSASLHAQDATA----NLFFS----- 661
            P S +DG     + K + PVLE   +DQ  K +Q+S +   ++ATA      FF+     
Sbjct: 611  PLSPSDGMVQPTQPKRKMPVLEKPLVDQLSKEEQDSLNTKFEEATAVDKKFSFFAFSIRR 670

Query: 662  -LLP-----YRRTVMQAVDLYDHYN--------------------VLHKSRCDNRLNEIT 721
             LLP     Y + +   V + D                       VL+KSRCDNR NEI 
Sbjct: 671  YLLPLPYGIYMKYLWICVAIVDELEKEIADSKQKIDFFRAKMQELVLYKSRCDNRYNEIA 730

Query: 722  ERASADKREAESLGKKYEEKYKQVAEIASKLTIEEAKYRDVQERKTELHQAIIKMEQGGS 781
            ER   DKRE ESL KKYEEKYK+   + SKLTIEEA +RD+QE+K EL+QAI+K E+G  
Sbjct: 731  ERVLGDKRELESLAKKYEEKYKKSGNVGSKLTIEEATFRDIQEKKMELYQAIVKFEEGKL 790

Query: 782  ADGILQVRADRIQSDIEELIKALTERCKKHGFDVKSAAIIELPVGWQPGIPDDAAIWDEE 841
             D I++ R + IQS +EELIK L ERCK++G   K  +++ELP GWQPGI + AA WDE+
Sbjct: 791  DDSIVK-RTEHIQSGLEELIKNLNERCKQYGVRGKPTSLVELPFGWQPGIQEGAADWDED 850

Query: 842  WDKFEDEGFS--NDLNLDPKGVSASKPKMSDS---EKDLADYNSTPDSSSNANGKMGNSL 901
            WDK EDEGF+   +L LD + V A   + S +   E D++       S S+A+ K G   
Sbjct: 851  WDKLEDEGFTFVKELTLDIQNVIAPPKEKSSAWRKEVDVSSKEGEDVSFSDADSKTGKKQ 910

Query: 902  STINRGLESESVYSHSEDESARSPYGS---PAAKTSLESPSH-EFSDAGYEKSPESYRSF 961
            S+     +SE     + D  AR   GS      +  +E+ S     D   E   +   S 
Sbjct: 911  SSGEE--DSEQSEGKTSDVDARDKNGSLDDSKVRKGIEADSSPRTKDTRSENGHDDGEST 970

Query: 962  NESAWGGTFDNNDDVDSVWGIKPVNTKEPDSEKHRDFFA-----SDFDTSSVKTGSPNAD 1021
              +     +D++D+ DSV  + P N K+ D  K+   F       DF    +KTGS  ++
Sbjct: 971  ASAGKTVNYDSHDETDSVSSVNPDNGKDKDHGKYDSGFGFGFGFDDFSIKPIKTGSTISN 1030

Query: 1022 SFFQRKSPFFEDSVP----------PTPLSRFGNSSPRYSDVGD---------------- 1067
             F   K   F DSVP          PT  S F +S P      +                
Sbjct: 1031 DFLPPKLSIFADSVPSPPANASDVSPTKPSLFADSVPSTPATNNASYPGQKSFFDDSVPS 1090

BLAST of Lsi04G018190 vs. TAIR 10
Match: AT4G05520.1 (EPS15 homology domain 2 )

HSP 1 Score: 57.8 bits (138), Expect = 6.3e-08
Identity = 35/111 (31.53%), Postives = 52/111 (46.85%), Query Frame = 0

Query: 4   FDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSVIKF 63
           +  +F  AD DGDGR+SG +A  FF  S L +  L QV                      
Sbjct: 20  YKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQV---------------------- 79

Query: 64  EATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAAL 115
                    W  AD ++ GFLG  EF  A++LV++AQ   E+T +++K ++
Sbjct: 80  ---------WAVADSKRQGFLGLSEFITAMKLVSLAQEGHEITSDLLKGSI 99

BLAST of Lsi04G018190 vs. TAIR 10
Match: AT4G05520.2 (EPS15 homology domain 2 )

HSP 1 Score: 57.8 bits (138), Expect = 6.3e-08
Identity = 35/111 (31.53%), Postives = 52/111 (46.85%), Query Frame = 0

Query: 4   FDAFFMRADLDGDGRISGAEAVAFFQGSNLPKNVLAQVANSKSTNCSFITSLLCFSVIKF 63
           +  +F  AD DGDGR+SG +A  FF  S L +  L QV                      
Sbjct: 20  YKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQV---------------------- 79

Query: 64  EATAPRGRIWMHADQRKTGFLGRPEFYNALRLVTVAQSKRELTPEIVKAAL 115
                    W  AD ++ GFLG  EF  A++LV++AQ   E+T +++K ++
Sbjct: 80  ---------WAVADSKRQGFLGLSEFITAMKLVSLAQEGHEITSDLLKGSI 99