Lsi04G017250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G017250
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionATP synthase subunit e, mitochondrial
Locationchr04: 24576094 .. 24578631 (+)
RNA-Seq ExpressionLsi04G017250
SyntenyLsi04G017250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGACTTGAGTGATGGCTTTTTAATCGGCGACCAGACGAGACGACTCGACGTCGGACGAAAGGGGTTCACTGATCTACCGTCGCGTCTGGTTTCTTCCTTCAATTAAAATTCATTATTCTATTGTTTTTCAATTACCGGTAAACCAGAGCCGATGCACGTCGTGGCATCGCGTAAGGCTTTTGGATTCTACGTGTCACTGTAAGAAGGGAAAGAAACGCTGCGTTGCTCTTCTGAGCCTCGGCCTCTACAATGACCCACATTTCCGATGGGCTTTCCCACTTTTATGGCATAATCTCATACGAAAAAAAAATAGTTGCAAATATAGCACTGAACGGAAAAATATTAGTGAATGTAGCATAATGAAAAAAAATATATATATAGAATTTCACTATATATATAGACCATTCTTTTTATCGTTATAAACTTAATTATTATGGTTAAAAATGTTACCTATTGTAATTTTAGATATTTAAACACTATTTTGGGTGTGTAAATCTCAAAATATTTTTTTTCTATAAGGAAATAAAATGTATTTTTTTTTCTTTTTGAGATAAATAGCAAACTAAAAATTAAATTGGGCTCTTTTCAAATATAAAATAATAAGTTAAATTATTTACAAATATAGAAAAATTTTAAGTGTATCAATGATAGACTGTAAAATTTTTCTATATTTGTAAATAGTTTGATATTTTTTTTGTTTATAGTAATTTTCCAATTAAATTTTATCTGAATTTGTGTATTATTTACCCTCTCATTTGACAGCCTCCTTCAACTTTTTTTTTTTTTTTTTTTTTTTCTTTGGTCTAGAGAAAAAGTCTTTCAACTTCTATACTAGATCACCAACGAAACGAGTGTTATTTAATTTGGTACTAGAAGAATAATTATAAGAATTTTACAATTTTAGACAGGGCAGGGAGGTCATTTACCAAAATTACCCTCGATTAGAGTTCGATATTCGGGCAGACGCACACGTCAGACTGTTAGAATTGATAGGTGACTCGGATCCAAAATTGCAGAGCAAATTAGGGTTCTACGATCCAGGTGGGAAACGGAAACTCATTGTCATTGGCATTCGAAGGAGGAGACGACTCCAAGCATTTCTGTGTTGTGACTGATAATCATCATTTACATCTAATGGCTCCACCTCCTGGCCCTTACTCCGGCACCAGCACCCTCGCTCTGGTATTTCCCCTTTCTTTTCCCCTTAGATTCTAATCATCTGCAGAACTACTTTCTCTTATATTTCCTTTTTTTATCCAGCTCGATCCTTGTGATGTCCCCTATCCACTTCAATACATCTAGATTTATCCGTTTCAAATCTTTTATTTTATTTTTCGAATTTTTTCATTATATTATTTTTAAATCCTGTCTTGTGAATTAAATGGATCGGTTCATTTTAGGTCCTAAAATTTTTGTCTCCTTTAATATCCTAATTTTTTGGGTATTGATTTCTACAGGTCGCTCGTGCATCAGCATTCACCTTTGGTTTGGTTTACGGAAGCATTAAGCTCAAGGTTCTGAAGGTAAATTGGAATCAACTATTGTCCAACCGTTGTTGTTATAGTAATACATTCGTTGAAATGTATTTTTGTAGGACATGGGTTTGATTGTATGGCGCCTCTGTTTACATCAACTCCATGTTTATTAATAATGTGACCATTTCACTCGTTCATTCACATGAATTAAACATTAAAACTAGATTTCATGTTATATGTTTCAAGGTTTTGACTGGTTTTCTTTTGGAGATGAGATTTGTTCATTTTCGTTTTTCATCGGCATTTCTAATTTATTGAAGAAGTCTTGTTGGAAACTTTAAAAGTTCAAATTTTGTGCCTCTTTATTGTCTCAAACGCGACATACTAATAAGTAATAATAATAAAAGAAACTGTTTACATCTATTCAGAGGCATTTAGATGAGACATGAGAGAGTTTCATCGGGTTCGTCAATTTCCATGCAATGTTATTTGTTTTTTAGATTAAATATGTGAAAAATTACAGCATGTAAGCACATGAAACATAGACAGCCTTATGTACCTTGATCGGTGAATTGTATAGAATATGAATTTGGTTGTGTAATAAGAATAACGTATCTTTTGGTTGTATTCTTTTGCAATCGCCAGGCAAAGGCCAAGTCACATGCAAAAGCTGAAGCCAAGGCCCATCATTGATGAACACATTTACTGATGTAGCTTGTAGCGATGGAAGAGATGGCAATTACCGCATAGGGAGAGACTCTGATCATTGGTTGGAGAAATGTGATTTTTGGCTTCATAATTTAGTAGATTCTGTTCTTTTACTGCTGAAACAAAAGAAAATTTTCTTTGTTTAGACGAGGTTGGTATTGCGAATACTGTATGGTGTATGCTTAGTTTTTCGGTTCCAGTTGGCAATTCTGCCTGTGAGAAGGATGTTCTTGAAATAAAGGCGATACACCATCCTGATAAATAAGACTCGTTTGAGATTTTTGTCCATCATGATACTTCTCTCGTTTACTTTAGAGTTTCAACTTTTGATTGGCTTTGATCTTTCTAAC

mRNA sequence

ATGAACGACTTGAGTGATGGCTTTTTAATCGGCGACCAGACGAGACGACTCGACGTCGGACGAAAGGGGTTCACTGATCTACCGTCGCGTCTGGGCAGGGAGGTCATTTACCAAAATTACCCTCGATTAGAGTTCGATATTCGGGCAGACGCACACGTCAGACTGTTAGAATTGATAGGAGGAGACGACTCCAAGCATTTCTGTGTTGTGACTGATAATCATCATTTACATCTAATGGCTCCACCTCCTGGCCCTTACTCCGGCACCAGCACCCTCGCTCTGGTCGCTCGTGCATCAGCATTCACCTTTGGTTTGGTTTACGGAAGCATTAAGCTCAAGGTTCTGAAGGCAAAGGCCAAGTCACATGCAAAAGCTGAAGCCAAGGCCCATCATTGATGAACACATTTACTGATGTAGCTTGTAGCGATGGAAGAGATGGCAATTACCGCATAGGGAGAGACTCTGATCATTGGTTGGAGAAATGTGATTTTTGGCTTCATAATTTAGTAGATTCTGTTCTTTTACTGCTGAAACAAAAGAAAATTTTCTTTGTTTAGACGAGGTTGGTATTGCGAATACTGTATGGTGTATGCTTAGTTTTTCGGTTCCAGTTGGCAATTCTGCCTGTGAGAAGGATGTTCTTGAAATAAAGGCGATACACCATCCTGATAAATAAGACTCGTTTGAGATTTTTGTCCATCATGATACTTCTCTCGTTTACTTTAGAGTTTCAACTTTTGATTGGCTTTGATCTTTCTAAC

Coding sequence (CDS)

ATGAACGACTTGAGTGATGGCTTTTTAATCGGCGACCAGACGAGACGACTCGACGTCGGACGAAAGGGGTTCACTGATCTACCGTCGCGTCTGGGCAGGGAGGTCATTTACCAAAATTACCCTCGATTAGAGTTCGATATTCGGGCAGACGCACACGTCAGACTGTTAGAATTGATAGGAGGAGACGACTCCAAGCATTTCTGTGTTGTGACTGATAATCATCATTTACATCTAATGGCTCCACCTCCTGGCCCTTACTCCGGCACCAGCACCCTCGCTCTGGTCGCTCGTGCATCAGCATTCACCTTTGGTTTGGTTTACGGAAGCATTAAGCTCAAGGTTCTGAAGGCAAAGGCCAAGTCACATGCAAAAGCTGAAGCCAAGGCCCATCATTGA

Protein sequence

MNDLSDGFLIGDQTRRLDVGRKGFTDLPSRLGREVIYQNYPRLEFDIRADAHVRLLELIGGDDSKHFCVVTDNHHLHLMAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH
Homology
BLAST of Lsi04G017250 vs. ExPASy TrEMBL
Match: A0A5A7V6P3 (ATP synthase E chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G008240 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.9e-19
Identity = 52/69 (75.36%), Postives = 57/69 (82.61%), Query Frame = 0

Query: 48  RADAHVRLLELIGGDDSKHFCVVTDNHHLHLMAPPPGPYSGTSTLALVARASAFTFGLVY 107
           R   H R +E  G    K+FC+ +DNHHLHLMAPPPGPYSGTSTLALVARASAFTFGLVY
Sbjct: 65  RNKGHKRCVE--GEGKFKYFCLASDNHHLHLMAPPPGPYSGTSTLALVARASAFTFGLVY 124

Query: 108 GSIKLKVLK 117
           G+IKLKVLK
Sbjct: 125 GNIKLKVLK 131

BLAST of Lsi04G017250 vs. ExPASy TrEMBL
Match: A0A6J1BT37 (ATP synthase subunit e, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005275 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.4e-18
Identity = 52/53 (98.11%), Postives = 53/53 (100.00%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPGPYSGTSTLALVARASAFTFG+VYGSIKLKVLKAKAKSHAKAEAKAHH
Sbjct: 1   MAPPPGPYSGTSTLALVARASAFTFGVVYGSIKLKVLKAKAKSHAKAEAKAHH 53

BLAST of Lsi04G017250 vs. ExPASy TrEMBL
Match: A0A1S3CRK7 (uncharacterized protein LOC103503894 OS=Cucumis melo OX=3656 GN=LOC103503894 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.1e-17
Identity = 51/53 (96.23%), Postives = 52/53 (98.11%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPGPYSGTSTLALVARASAFTFGLVYG+IKLKVLKAKAKS AKAEAKAHH
Sbjct: 1   MAPPPGPYSGTSTLALVARASAFTFGLVYGNIKLKVLKAKAKSRAKAEAKAHH 53

BLAST of Lsi04G017250 vs. ExPASy TrEMBL
Match: A0A0A0LH44 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G866500 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.7e-17
Identity = 51/53 (96.23%), Postives = 52/53 (98.11%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPGPYSGTSTLALVARASAF+FGLVYGSIKLKVLKAKAKS AKAEAKAHH
Sbjct: 1   MAPPPGPYSGTSTLALVARASAFSFGLVYGSIKLKVLKAKAKSRAKAEAKAHH 53

BLAST of Lsi04G017250 vs. ExPASy TrEMBL
Match: A0A2N9G4U9 (ATP synthase subunit e, mitochondrial OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25569 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.6e-17
Identity = 50/53 (94.34%), Postives = 51/53 (96.23%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPGPYSGTSTLALVARASAF+FGLVYGSIKLK LKAKAKSH KAEAKAHH
Sbjct: 1   MAPPPGPYSGTSTLALVARASAFSFGLVYGSIKLKYLKAKAKSHQKAEAKAHH 53

BLAST of Lsi04G017250 vs. NCBI nr
Match: KAA0063268.1 (ATP synthase E chain [Cucumis melo var. makuwa] >TYK31506.1 ATP synthase E chain [Cucumis melo var. makuwa])

HSP 1 Score: 104.8 bits (260), Expect = 5.9e-19
Identity = 52/69 (75.36%), Postives = 57/69 (82.61%), Query Frame = 0

Query: 48  RADAHVRLLELIGGDDSKHFCVVTDNHHLHLMAPPPGPYSGTSTLALVARASAFTFGLVY 107
           R   H R +E  G    K+FC+ +DNHHLHLMAPPPGPYSGTSTLALVARASAFTFGLVY
Sbjct: 65  RNKGHKRCVE--GEGKFKYFCLASDNHHLHLMAPPPGPYSGTSTLALVARASAFTFGLVY 124

Query: 108 GSIKLKVLK 117
           G+IKLKVLK
Sbjct: 125 GNIKLKVLK 131

BLAST of Lsi04G017250 vs. NCBI nr
Match: XP_022132454.1 (uncharacterized protein LOC111005275 [Momordica charantia])

HSP 1 Score: 101.7 bits (252), Expect = 5.0e-18
Identity = 52/53 (98.11%), Postives = 53/53 (100.00%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPGPYSGTSTLALVARASAFTFG+VYGSIKLKVLKAKAKSHAKAEAKAHH
Sbjct: 1   MAPPPGPYSGTSTLALVARASAFTFGVVYGSIKLKVLKAKAKSHAKAEAKAHH 53

BLAST of Lsi04G017250 vs. NCBI nr
Match: XP_038898502.1 (uncharacterized protein LOC120086120 [Benincasa hispida])

HSP 1 Score: 99.8 bits (247), Expect = 1.9e-17
Identity = 52/53 (98.11%), Postives = 52/53 (98.11%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MA PPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH
Sbjct: 1   MATPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 53

BLAST of Lsi04G017250 vs. NCBI nr
Match: XP_008466504.1 (PREDICTED: uncharacterized protein LOC103503894 [Cucumis melo])

HSP 1 Score: 98.6 bits (244), Expect = 4.2e-17
Identity = 51/53 (96.23%), Postives = 52/53 (98.11%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPGPYSGTSTLALVARASAFTFGLVYG+IKLKVLKAKAKS AKAEAKAHH
Sbjct: 1   MAPPPGPYSGTSTLALVARASAFTFGLVYGNIKLKVLKAKAKSRAKAEAKAHH 53

BLAST of Lsi04G017250 vs. NCBI nr
Match: XP_004147837.1 (uncharacterized protein LOC101217057 [Cucumis sativus])

HSP 1 Score: 98.2 bits (243), Expect = 5.5e-17
Identity = 51/53 (96.23%), Postives = 52/53 (98.11%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPGPYSGTSTLALVARASAF+FGLVYGSIKLKVLKAKAKS AKAEAKAHH
Sbjct: 1   MAPPPGPYSGTSTLALVARASAFSFGLVYGSIKLKVLKAKAKSRAKAEAKAHH 53

BLAST of Lsi04G017250 vs. TAIR 10
Match: AT3G01130.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: male gametophyte, pollen tube; EXPRESSED DURING: M germinated pollen stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G15320.1); Has 64 Blast hits to 64 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 64; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 80.1 bits (196), Expect = 1.5e-15
Identity = 42/53 (79.25%), Postives = 44/53 (83.02%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           MAPPPG YSGTSTLALVARASAF  GL+YG+IKLK LK K  S  KAEAKAHH
Sbjct: 1   MAPPPGLYSGTSTLALVARASAFGLGLIYGNIKLKALKIKKNSQIKAEAKAHH 53

BLAST of Lsi04G017250 vs. TAIR 10
Match: AT5G15320.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01130.1); Has 64 Blast hits to 64 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 64; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-12
Identity = 38/53 (71.70%), Postives = 42/53 (79.25%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           M  PPG YSGTS+LALVARASAF  GLVYG++KLKVLK K+ S  K EA AHH
Sbjct: 1   MTLPPGLYSGTSSLALVARASAFGLGLVYGNMKLKVLKIKSMSQKKVEATAHH 53

BLAST of Lsi04G017250 vs. TAIR 10
Match: AT3G01130.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: male gametophyte, pollen tube; EXPRESSED DURING: M germinated pollen stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G15320.1); Has 64 Blast hits to 64 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 64; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 65.1 bits (157), Expect = 4.8e-11
Identity = 33/40 (82.50%), Postives = 35/40 (87.50%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAK 119
           MAPPPG YSGTSTLALVARASAF  GL+YG+IKLK LK K
Sbjct: 1   MAPPPGLYSGTSTLALVARASAFGLGLIYGNIKLKALKHK 40

BLAST of Lsi04G017250 vs. TAIR 10
Match: AT5G15320.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01130.1); Has 64 Blast hits to 64 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 64; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 62.0 bits (149), Expect = 4.1e-10
Identity = 35/53 (66.04%), Postives = 40/53 (75.47%), Query Frame = 0

Query: 79  MAPPPGPYSGTSTLALVARASAFTFGLVYGSIKLKVLKAKAKSHAKAEAKAHH 132
           M  PPG YSGTS+LALVARASAF  GLVYG++KLK+   K+ S  K EA AHH
Sbjct: 1   MTLPPGLYSGTSSLALVARASAFGLGLVYGNMKLKI---KSMSQKKVEATAHH 50

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V6P32.9e-1975.36ATP synthase E chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455... [more]
A0A6J1BT372.4e-1898.11ATP synthase subunit e, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A1S3CRK72.1e-1796.23uncharacterized protein LOC103503894 OS=Cucumis melo OX=3656 GN=LOC103503894 PE=... [more]
A0A0A0LH442.7e-1796.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G866500 PE=4 SV=1[more]
A0A2N9G4U94.6e-1794.34ATP synthase subunit e, mitochondrial OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25... [more]
Match NameE-valueIdentityDescription
KAA0063268.15.9e-1975.36ATP synthase E chain [Cucumis melo var. makuwa] >TYK31506.1 ATP synthase E chain... [more]
XP_022132454.15.0e-1898.11uncharacterized protein LOC111005275 [Momordica charantia][more]
XP_038898502.11.9e-1798.11uncharacterized protein LOC120086120 [Benincasa hispida][more]
XP_008466504.14.2e-1796.23PREDICTED: uncharacterized protein LOC103503894 [Cucumis melo][more]
XP_004147837.15.5e-1796.23uncharacterized protein LOC101217057 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G01130.11.5e-1579.25unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G15320.11.2e-1271.70unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G01130.24.8e-1182.50unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G15320.24.1e-1066.04unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR36028:SF4SUBFAMILY NOT NAMEDcoord: 79..131
NoneNo IPR availablePANTHERPTHR36028OSJNBB0050O03.8 PROTEINcoord: 79..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G017250.1Lsi04G017250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015986 ATP synthesis coupled proton transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)
molecular_function GO:0015078 proton transmembrane transporter activity