Lsi04G016900 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G016900
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	chr04: 24289811 .. 24293238 (+)
RNA-Seq Expression	Lsi04G016900
Synteny	Lsi04G016900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCATTATATAATTCGCTAATTCCCAGCACTTAATCTAGCCTTCGTGCTTCTCCTTACACAACCAACTACTGCATTTTCACTTTCACAGACTCTGTTCACAGAGAAAAAAACAGAGAGATGAAGCTTTGGAGTTCCATTTTGGTTCTTCTTCTACCTCTCGTGCTTCTAGCAGAAGCAGATGATGGGTTTGTAAGAACCAGAGGACAGCAATTGATTCTGAATGGAACTCCATTTTACGCCAATGGGTTCAATGCCTATTGGTTGATGTACTTCGCTTCAGACCCATCACAGAGAACCAAAGTCTCCTCTGCTTTTCAACAAGCCGTCAATCACGGCCTCTCCATTGGAAGAACTTGGGCCTTCAGCGATGGCGGATACAGCCCTCTACAGTACTCTCCAGGCCAATACAATGAGAAGATGTTGCAGGTTCTTCGATTCCTTTGATTTTTCATTTTCCTCTGTTTTTAGTGTTTTTTTCCCTCCTTTTGCTAAATGGGAAAATGGGTTTTGTTAGGGATTGGATTTTGTGGTATCGGAAGCAAGAAAATATGGGATTAAATTGATATTGAGTTTGGTGAACAACTATGAAAGTATGGGAGGGAAAAAACAGTATGTGGAGTGGGCAAGAAGCCAGGGGCAGTCCATTTCATCTGAGGATGATTTCTTCACTAATCCTGTTGTGAAAGGGTTCTACAAGAACCATATTAAGGTAAAATCAGAAGACGTAATCAAAATTCACTTCTAATTTCTGAACTGTCATTTTCCATTTTTCTCTTTTGTTGGTGAAATTGGTTGAAATTTTGAAAAGGGTTTGTGAATGATTTAAAGTTTATGCACTGAGCTTGTGAGGAGGGGCTGGTGAAAGTGAAACGGGTGGTTGGAGTCTTGGAGAAGAGTGGTTGGGAATTGGAACAGGTCATTTACAGAGGGGAGTAATTATGAAATTTTACTTTTTTTTTTTTAAAAAAAAAATTCCACTCTAAAATCATTTCCGTTGAGGAATGTATGGAATGTTTTCCATGTGCAATTTGAACTCAACGAACAATTATGATCTATGAAGAGAGTTTTTTTTCCTCTTTCTTTTCACTGAAGATTGAGCTATCCCAAATTTTATTTTCAACTAATTGAAACATGAACTTAATAAAGGTGAAATTATGTTTAATTAATTTATATTTTTATGAAAAATCAAGCATTTTGCGTCGAGACTTCTTTTTATGGGTTAGTTTATACTTTTAGGTGGGGTTTGAGAATTTTTTTTTTAATTAAATTTTTAGAGATAGAAAAAATGTCAAACTATTTAGAGAAAATAACAAAAAAAAAAAACACTGATAGACTTCTATCAGCATCTATCAGTGAAAGATTTCTATCTTTCTATCACTTACAGACCTTCGTAAACTTCATCTATCACAATTGTCTAAAAATTTTGCTATTTTGTGTTGATAGTTTTCCTTGTTTTTCTGTTTTTAAAAATTTCTGTTTTTCATTTTTTATTTTACAGTCTCAATCTGTTTAATATTTTATTAGTTGAATTTGCTGAGATTTATCATTTGTTATATATTAGGATCAAAAGTGACTTTATTTACGATGTTGCCCAAATTGCCCATTTTGTGGTGCTAGTTGAAGTCGATATTGTGACTTTATTGTGATTTCCACCTTTTATGGAAACCTTGTTTAAATTTTTCTCAACTTGGCAGGTAATAAATTGAATTCATAGAAATTGGTATGAAATTCAATGATAAAATCAATTTTCTTAATAGTCCTTTCTTTGTTCCAATTACCATATCCATGAAGTTGTAAGTAGGTACAATATCTATCCTCCTTTAAAAAATGAGCAATACTTGAGTGCAATCTATGCTACCATTCATCTCCTTCAATCACAAAGGAAAAAAAAACATTTTTTTAGAAAGAAAAGAAAAATTATCACGTGACAACTTGTGATTAGACATTGTGTGGGATGCACAAAGATAAGATGAACCTAAATATTTTTCTTAAAAAAATTCCTAAATTCAAATTTCTTTGCCCTACACAGTAATTTAAGAAAACAACAGGAAGTATACATTAATTTGTGGATTAAAATTATAAATATTGATAATTTCGTTTGAATAATACATATTGTATATAGAGCATTTTGACAAGAACCAACAGCATCACCGGAGTAGCTTATAAGGATGATCCAACCATAATGGCTTGGGAACTCATGAACGAAGCCAGATGCCCTTCAGATCCTTCAGGGAACACCATTCAGGTTCTCACTACATATACCCTTCTTTCCTAATATTTTTCCTTTTCAATCTTTAATTTCAACCTTAAATATTTCATTCATTCCAAAAACCAATTTTAATCTTGATTTGGACCAAACCTGATTGTAAATTTATACCTACTAAGTTGACATAGCCAAATTTAAAAAGCTGTGTGTATGAATTGATGGGCAGACATGGATTAGAGAAATGGCATCATACTTGAAATCAATAGATGGTAAACATCTATTAGAAGCTGGTTTGGAAGGTTTCTATGGACAATCAAAGTATCAAGGGAATCCTAATTTTCAAGTGGGAACTGATTTCATTGCAAACAATCAGATACCTGAACTTGACTTTGCCACAGTTCACTCATATCCTGACCAATGGTAAGAACAAATCTTTCCCTATTTGCTTCTTTTTGGGATCTTTAATCACCAATAGCTAAAATGAAATGGGTAATTTCTACTGATGTTGTAGGTTATCTGGTTCTAGTTATGAGAATCAGCTCTCATTCCTCAACACTTGGCTCAATGATCACATCCAAGATGCACAGAACATTCTGCACAAGCCAGTTCTTTTTGCTGAGTTTGGAAAATCTACAAAGTACTCTGGCGCTGATCAAAGGGATCAGCTTTTCAATGCTGTTTACTCAGCTGTGTATTCGTCAGCTCGGGGAGGCGGTGCAGCCATCGGTGGACTGTTCTGGCAACTTCTAGTTGAAGGGATGGATTCTTTTCGGGATGGGTATGAAGTGGTACTTAGTGAGAACCCATCAACTGCTAATTTGATTTCTCAGGAGTCTAGAAGGCTGATTCACATCCGGAAGATGTATGCCAAACTCAGAAACATTGAGAAGTGGAAAAGAGCAAGGGAGATAAGAAAAGCACAGTGGGAGGCCTTAAAGGGAGGCAACAAGAGTCCTGGAAATTAAGTAAAAATAGAGCTGAAAATAGAAACGCTAAGCAAATATTGACAACTTAATATAACTTGTGAGTTTAAGAGAAACTCCCTCACAGAGATGAGATTTGCTTGTCCGAAAGCTGATGTTAAATGGCTTTGAATATTGTCTTCTCTTGTGATAAATGGATTGAAATCAAAAGTTATTACTCAGGATGGAAATGTGTTGGTTTTCTGCTTTTTACATATTTGAAAAGAAACTAGTATTTTACATCAAAGCCAATTACT

mRNA sequence

CTTTCATTATATAATTCGCTAATTCCCAGCACTTAATCTAGCCTTCGTGCTTCTCCTTACACAACCAACTACTGCATTTTCACTTTCACAGACTCTGTTCACAGAGAAAAAAACAGAGAGATGAAGCTTTGGAGTTCCATTTTGGTTCTTCTTCTACCTCTCGTGCTTCTAGCAGAAGCAGATGATGGGTTTGTAAGAACCAGAGGACAGCAATTGATTCTGAATGGAACTCCATTTTACGCCAATGGGTTCAATGCCTATTGGTTGATGTACTTCGCTTCAGACCCATCACAGAGAACCAAAGTCTCCTCTGCTTTTCAACAAGCCGTCAATCACGGCCTCTCCATTGGAAGAACTTGGGCCTTCAGCGATGGCGGATACAGCCCTCTACAGTACTCTCCAGGCCAATACAATGAGAAGATGTTGCAGGGATTGGATTTTGTGGTATCGGAAGCAAGAAAATATGGGATTAAATTGATATTGAGTTTGGTGAACAACTATGAAAGTATGGGAGGGAAAAAACAGTATGTGGAGTGGGCAAGAAGCCAGGGGCAGTCCATTTCATCTGAGGATGATTTCTTCACTAATCCTGTTGTGAAAGGGTTCTACAAGAACCATATTAAGAGCATTTTGACAAGAACCAACAGCATCACCGGAGTAGCTTATAAGGATGATCCAACCATAATGGCTTGGGAACTCATGAACGAAGCCAGATGCCCTTCAGATCCTTCAGGGAACACCATTCAGACATGGATTAGAGAAATGGCATCATACTTGAAATCAATAGATGGTAAACATCTATTAGAAGCTGGTTTGGAAGGTTTCTATGGACAATCAAAGTATCAAGGGAATCCTAATTTTCAAGTGGGAACTGATTTCATTGCAAACAATCAGATACCTGAACTTGACTTTGCCACAGTTCACTCATATCCTGACCAATGGTTATCTGGTTCTAGTTATGAGAATCAGCTCTCATTCCTCAACACTTGGCTCAATGATCACATCCAAGATGCACAGAACATTCTGCACAAGCCAGTTCTTTTTGCTGAGTTTGGAAAATCTACAAAGTACTCTGGCGCTGATCAAAGGGATCAGCTTTTCAATGCTGTTTACTCAGCTGTGTATTCGTCAGCTCGGGGAGGCGGTGCAGCCATCGGTGGACTGTTCTGGCAACTTCTAGTTGAAGGGATGGATTCTTTTCGGGATGGGTATGAAGTGGTACTTAGTGAGAACCCATCAACTGCTAATTTGATTTCTCAGGAGTCTAGAAGGCTGATTCACATCCGGAAGATGTATGCCAAACTCAGAAACATTGAGAAGTGGAAAAGAGCAAGGGAGATAAGAAAAGCACAGTGGGAGGCCTTAAAGGGAGGCAACAAGAGTCCTGGAAATTAAGTAAAAATAGAGCTGAAAATAGAAACGCTAAGCAAATATTGACAACTTAATATAACTTGTGAGTTTAAGAGAAACTCCCTCACAGAGATGAGATTTGCTTGTCCGAAAGCTGATGTTAAATGGCTTTGAATATTGTCTTCTCTTGTGATAAATGGATTGAAATCAAAAGTTATTACTCAGGATGGAAATGTGTTGGTTTTCTGCTTTTTACATATTTGAAAAGAAACTAGTATTTTACATCAAAGCCAATTACT

Coding sequence (CDS)

ATGAAGCTTTGGAGTTCCATTTTGGTTCTTCTTCTACCTCTCGTGCTTCTAGCAGAAGCAGATGATGGGTTTGTAAGAACCAGAGGACAGCAATTGATTCTGAATGGAACTCCATTTTACGCCAATGGGTTCAATGCCTATTGGTTGATGTACTTCGCTTCAGACCCATCACAGAGAACCAAAGTCTCCTCTGCTTTTCAACAAGCCGTCAATCACGGCCTCTCCATTGGAAGAACTTGGGCCTTCAGCGATGGCGGATACAGCCCTCTACAGTACTCTCCAGGCCAATACAATGAGAAGATGTTGCAGGGATTGGATTTTGTGGTATCGGAAGCAAGAAAATATGGGATTAAATTGATATTGAGTTTGGTGAACAACTATGAAAGTATGGGAGGGAAAAAACAGTATGTGGAGTGGGCAAGAAGCCAGGGGCAGTCCATTTCATCTGAGGATGATTTCTTCACTAATCCTGTTGTGAAAGGGTTCTACAAGAACCATATTAAGAGCATTTTGACAAGAACCAACAGCATCACCGGAGTAGCTTATAAGGATGATCCAACCATAATGGCTTGGGAACTCATGAACGAAGCCAGATGCCCTTCAGATCCTTCAGGGAACACCATTCAGACATGGATTAGAGAAATGGCATCATACTTGAAATCAATAGATGGTAAACATCTATTAGAAGCTGGTTTGGAAGGTTTCTATGGACAATCAAAGTATCAAGGGAATCCTAATTTTCAAGTGGGAACTGATTTCATTGCAAACAATCAGATACCTGAACTTGACTTTGCCACAGTTCACTCATATCCTGACCAATGGTTATCTGGTTCTAGTTATGAGAATCAGCTCTCATTCCTCAACACTTGGCTCAATGATCACATCCAAGATGCACAGAACATTCTGCACAAGCCAGTTCTTTTTGCTGAGTTTGGAAAATCTACAAAGTACTCTGGCGCTGATCAAAGGGATCAGCTTTTCAATGCTGTTTACTCAGCTGTGTATTCGTCAGCTCGGGGAGGCGGTGCAGCCATCGGTGGACTGTTCTGGCAACTTCTAGTTGAAGGGATGGATTCTTTTCGGGATGGGTATGAAGTGGTACTTAGTGAGAACCCATCAACTGCTAATTTGATTTCTCAGGAGTCTAGAAGGCTGATTCACATCCGGAAGATGTATGCCAAACTCAGAAACATTGAGAAGTGGAAAAGAGCAAGGGAGATAAGAAAAGCACAGTGGGAGGCCTTAAAGGGAGGCAACAAGAGTCCTGGAAATTAA

Protein sequence

MKLWSSILVLLLPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSKYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGNKSPGN

Homology

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BLAST of Lsi04G016900 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.1e-157
Identity = 266/403 (66.00%), Postives = 322/403 (79.90%), Query Frame = 0


Query: 23  GFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIGRTWAF 82
           GFVRT+G Q  LNG P+YANGFNAYWLMY ASDPSQR+K+S+AFQ A  HGL++ RTWAF
Sbjct: 30  GFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAF 89

Query: 83  SDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYVEWARS 142
           SDGGY  LQYSPG YNE M QGLDF ++EAR++GIK+ILS  NNYES GG+KQYV+WARS
Sbjct: 90  SDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARS 149

Query: 143 QGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEARCPSD 202
           +G+ +SSEDDFFT+ +VK FYKNHIK++L R N+ T V YKDDPTIMAWELMNE RCPSD
Sbjct: 150 RGRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSD 209

Query: 203 PSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSKYQG---NPNFQVGTDFIANNQI 262
           PSG  IQ WI EMA+++KS+D  HLLEAGLEGFYGQS  Q    NP  Q GTDFIANN+I
Sbjct: 210 PSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPGQFGTDFIANNRI 269

Query: 263 PELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSG 322
           P +DF TVHSYPD+W   SS ++Q+ FLN WL+ HIQDAQN+LHKP++ AEFGKS K  G
Sbjct: 270 PGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPG 329

Query: 323 --ADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSENPSTANL 382
               QRD +FN VYS +Y SA+ GGAA GGLFWQLLV G+D+F+DGY ++LS++ ST N+
Sbjct: 330 YTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNV 389

Query: 383 ISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGNK 421
           ISQ+SR+L  IRK++A++ N+EKWKRAR     Q +  K G+K
Sbjct: 390 ISQQSRKLTLIRKIFARMINVEKWKRAR----GQGQVGKRGHK 428

[Close]

BLAST of Lsi04G016900 vs. ExPASy Swiss-Prot
Match: Q0JKM9 (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN1 PE=2 SV=2)

HSP 1 Score: 514.2 bits (1323), Expect = 1.4e-144
Identity = 254/412 (61.65%), Postives = 314/412 (76.21%), Query Frame = 0


Query: 5   SSILVLLLPLVL----LAEAD-----DGFVRTRGQQLILNGTPFYANGFNAYWLMYFASD 64
           +++LVL   +V+    L EA+      GFVR +G + +L+G P+YANGFNAYWLM  A+D
Sbjct: 9   AALLVLACVVVVVIHGLGEAEALGGGGGFVRAQGTRFVLDGNPYYANGFNAYWLMLLAAD 68

Query: 65  PSQRTKVSSAFQQAVNHGLSIGRTWAFSD-GGYSPLQYSPGQYNEKMLQGLDFVVSEARK 124
           PSQR KVS+A  +A  HGL++ RTWAFSD GG + LQ SPG YNE   +GLDFV+SEARK
Sbjct: 69  PSQRGKVSAALGEAAGHGLTVARTWAFSDGGGGNALQLSPGNYNENTFKGLDFVLSEARK 128

Query: 125 YGIKLILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRT 184
           YGIK+ILSLV+NY+S GG+KQYV WAR+QGQ I S+D+FFTNPVVKGFYKNH+K++LTR 
Sbjct: 129 YGIKVILSLVDNYDSFGGRKQYVNWARAQGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRK 188

Query: 185 NSITGVAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEG 244
           N+ITGVAY+DDPTI+AWELMNE RC SD SG T+Q+WI EMA+++KSID  H+LE GLEG
Sbjct: 189 NTITGVAYRDDPTILAWELMNEPRCQSDLSGRTVQSWITEMAAHVKSIDRNHMLEVGLEG 248

Query: 245 FYGQSKYQG----NPN-FQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNT 304
           FYG S        NP+ +Q+GTDFIANNQ+P +DFATVHSYPDQWLSG   + QL F+  
Sbjct: 249 FYGASSPSRIAAVNPSGYQLGTDFIANNQVPGIDFATVHSYPDQWLSGKDDQAQLGFMGR 308

Query: 305 WLNDHIQDAQNILHKPVLFAEFGKSTKYSG--ADQRDQLFNAVYSAVYSSARGGGAAIGG 364
           WL+ HI DAQ +L KP+L AEFGKS K  G  + QRD L+  VY+ +Y SAR GGA +GG
Sbjct: 309 WLDAHIADAQAVLRKPLLIAEFGKSWKDPGYSSGQRDALYGTVYAKIYESARRGGATVGG 368

Query: 365 LFWQLLVEGMDSFRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIE 400
           LFWQLLV GMDS+RDGYEVV  E PST  +I+  SRRL  + K +A+ R  +
Sbjct: 369 LFWQLLVPGMDSYRDGYEVVFGETPSTTGVITTNSRRLRFLSKAFARARQAQ 420

[Close]

BLAST of Lsi04G016900 vs. ExPASy Swiss-Prot
Match: Q6Z310 (Putative mannan endo-1,4-beta-mannosidase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN9 PE=2 SV=2)

HSP 1 Score: 459.1 bits (1180), Expect = 5.3e-128
Identity = 212/369 (57.45%), Postives = 273/369 (73.98%), Query Frame = 0


Query: 20  ADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIGRT 79
           A+  F R  G +  + G PFY+NGFNAYWLMY ASDP  R+K +   QQA +   ++ RT
Sbjct: 35  ANGQFARASGTRFTVGGRPFYSNGFNAYWLMYMASDPGDRSKAAGVLQQAASLRATLVRT 94

Query: 80  WAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYVEW 139
           WAFSDGGY PLQ SPG YNE M  GLDFV++EA+K G+ LILSLVNN++  GGKKQYV+W
Sbjct: 95  WAFSDGGYRPLQKSPGVYNEDMFMGLDFVIAEAKKRGLYLILSLVNNWDGFGGKKQYVQW 154

Query: 140 ARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEARC 199
           AR QG ++ S+DDFF + V K FYKNH  ++LTR N ITGVAYKDDPTI AWEL+NE RC
Sbjct: 155 ARDQGHNLGSDDDFFRSDVTKQFYKNH--AVLTRVNKITGVAYKDDPTIFAWELINEPRC 214

Query: 200 PSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSKYQG-NPNFQVGTDFIANNQ 259
            SD SG T+Q W+ EMA Y+KS+D  H++E GLEGFYG+S ++  NP + VGTDFIANN 
Sbjct: 215 QSDLSGKTLQAWVTEMAGYVKSVDPNHMVEIGLEGFYGESMHKNFNPGYTVGTDFIANNL 274

Query: 260 IPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYS 319
           +P +DFAT+HSYPDQW+SG+S + Q++F+  W+ DHI+D+  +L KP+L  EFG S + +
Sbjct: 275 VPAVDFATIHSYPDQWVSGASSDEQVAFMRKWMADHIRDSAAVLRKPLLVTEFGWSARSN 334

Query: 320 G--ADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSENPSTAN 379
           G     RD  F  VY AVY+SAR GGA  GGLFWQ++  GM+S+ DGYEVVL  + STA+
Sbjct: 335 GYTVAARDAYFRTVYDAVYASAREGGACAGGLFWQVMAPGMESWTDGYEVVLERSKSTAD 394

Query: 380 LISQESRRL 386
           +++ +  R+
Sbjct: 395 VVAHQCARI 401

[Close]

BLAST of Lsi04G016900 vs. ExPASy Swiss-Prot
Match: Q8L5J1 (Mannan endo-1,4-beta-mannosidase 4 OS=Solanum lycopersicum OX=4081 GN=MAN4 PE=1 SV=2)

HSP 1 Score: 457.2 bits (1175), Expect = 2.0e-127
Identity = 217/371 (58.49%), Postives = 275/371 (74.12%), Query Frame = 0


Query: 18  AEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIG 77
           A +++ FV T G    LNG   Y NGFNAYWLMY A DPS R KV++ FQQA  + +++ 
Sbjct: 26  AFSNNNFVYTDGTHFALNGKSLYINGFNAYWLMYIAYDPSTRIKVTNTFQQASKYKMNVA 85

Query: 78  RTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYV 137
           RTWAFS GG  PLQ +PG YNE+M QGLDFV+SEA+KYGI LI+SLVNN+++ GGKKQYV
Sbjct: 86  RTWAFSHGGSRPLQSAPGVYNEQMFQGLDFVISEAKKYGIHLIMSLVNNWDAFGGKKQYV 145

Query: 138 EWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEA 197
           EWA  +GQ ++S+DDFFTNP+VKGFYKN++K +LTR N+IT VAYKDDPTI++WEL+NE 
Sbjct: 146 EWAVQRGQKLTSDDDFFTNPMVKGFYKNNVKVVLTRVNTITKVAYKDDPTILSWELINEP 205

Query: 198 RCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSKYQGNPNFQV-GTDFIAN 257
           RCPSD SG T Q W+ EMA YLKSID  HLLE GLEGFYG    Q NPN  + GT+FI+N
Sbjct: 206 RCPSDLSGKTFQNWVLEMAGYLKSIDSNHLLEIGLEGFYGNDMRQYNPNSYIFGTNFISN 265

Query: 258 NQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTK 317
           NQ+  +DF T+H YP+QWL G + E Q  + + W+  HI D++ +L KP+L AEFGKSTK
Sbjct: 266 NQVQGIDFTTIHMYPNQWLPGLTQEAQDKWASQWIQVHIDDSK-MLKKPLLIAEFGKSTK 325

Query: 318 YSG--ADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSENPST 377
             G    +RD  F  +Y  +++ A+ GG   GGLFWQ+L +GM SF DGY+VVL E+PST
Sbjct: 326 TPGYTVAKRDNYFEKIYGTIFNCAKSGGPCGGGLFWQVLGQGMSSFDDGYQVVLQESPST 385

Query: 378 ANLISQESRRL 386
           + +I  +S RL
Sbjct: 386 SRVILLQSLRL 395

[Close]

BLAST of Lsi04G016900 vs. ExPASy Swiss-Prot
Match: Q9FZ03 (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 448.0 bits (1151), Expect = 1.2e-124
Identity = 217/400 (54.25%), Postives = 285/400 (71.25%), Query Frame = 0


Query: 2   KLWSSILVL-LLPLVLLAEA----------DDGFVRTRGQQLILNGTPFYANGFNAYWLM 61
           +L S I VL LL L    EA          D GFVR  G    LNG+PF  NGFN+YWLM
Sbjct: 6   RLISCIFVLFLLSLAFACEARVLLDENNANDQGFVRVNGAHFELNGSPFLFNGFNSYWLM 65

Query: 62  YFASDPSQRTKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVS 121
           + A++PS+R KVS   ++A + GLS+ RTWAFSDGG   LQ SPG Y+E++ QGLDFV+S
Sbjct: 66  HVAAEPSERYKVSEVLREASSAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVIS 125

Query: 122 EARKYGIKLILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSI 181
           EA+KYGI+LILS VNNY   GGK QYV+WAR+ G  I+ +DDF+TN + K +YKNHIK +
Sbjct: 126 EAKKYGIRLILSFVNNYNDFGGKAQYVQWARNAGAQINGDDDFYTNYITKNYYKNHIKKV 185

Query: 182 LTRTNSITGVAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEA 241
           +TR N+ITG+ YKDD TIMAWELMNE R  +D SGNT+  W++EMAS++KS+D KHLLE 
Sbjct: 186 VTRFNTITGMTYKDDSTIMAWELMNEPRNQADYSGNTLNAWVQEMASFVKSLDNKHLLEI 245

Query: 242 GLEGFYGQS---KYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFL 301
           G+EGFYG S   +   NP +QVGTDFI+N+ I E+DFAT+H+Y DQWLSG S + Q+ F+
Sbjct: 246 GMEGFYGDSVPERKSINPGYQVGTDFISNHLIKEIDFATIHAYTDQWLSGQSDDAQMIFM 305

Query: 302 NTWLNDHIQDAQNILHKPVLFAEFGKSTKYSGADQ--RDQLFNAVYSAVYSSARGGGAAI 361
             W+  H QDA+NIL KP++ AEFGKS++  G +Q  RD   + +Y  +YS A+ GG   
Sbjct: 306 QKWMTSHWQDAKNILKKPLVLAEFGKSSRDPGYNQNIRDTFMSTIYRNIYSLAKDGGTMG 365

Query: 362 GGLFWQLLVEGMDSFRDGYEVVLSENPSTANLISQESRRL 386
           G L WQL+ +GM+++ DGY + L +NPSTA +I+ +S  +
Sbjct: 366 GSLIWQLVAQGMENYEDGYCIELGKNPSTAGIITSQSHAM 405

[Close]

BLAST of Lsi04G016900 vs. ExPASy TrEMBL
Match: E5GCI6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 6.5e-230
Identity = 393/425 (92.47%), Postives = 412/425 (96.94%), Query Frame = 0


Query: 1   MKLWSSILVLL-LPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQR 60
           MKL  SI +LL LPLVLLA+ADDGFVRT+GQQLILNGTPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  TKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKL 120
            KVSSAFQQAV+HGLSIGRTWAF+DGGYSPLQYSPGQYNEKM QGLDFVV+EARKYGIKL
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITG 180
           ILSLVNNYESMGGKKQYVEWARSQGQ+ISSED+FFTNPVVKGFYKNHIKSILTR NSITG
Sbjct: 121 ILSLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQ WI+EMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQ NPNFQVGTDFIANNQIPE+DFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDS 360
           NILHKPVLFAEFGKS KYSGADQRDQLFNAVYSAVYSSARGGGAAIGG+FWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGN 420
           FRDGYEVVLSE+PSTANLI+QESRRLIHIR+MYAKLRNIEKWKRA+E++KAQWEA +GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGN 420

Query: 421 KSPGN 425
            SPGN
Sbjct: 421 NSPGN 425

[Close]

BLAST of Lsi04G016900 vs. ExPASy TrEMBL
Match: A0A1S3CRH7 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103503926 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 6.5e-230
Identity = 393/425 (92.47%), Postives = 412/425 (96.94%), Query Frame = 0


Query: 1   MKLWSSILVLL-LPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQR 60
           MKL  SI +LL LPLVLLA+ADDGFVRT+GQQLILNGTPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  TKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKL 120
            KVSSAFQQAV+HGLSIGRTWAF+DGGYSPLQYSPGQYNEKM QGLDFVV+EARKYGIKL
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITG 180
           ILSLVNNYESMGGKKQYVEWARSQGQ+ISSED+FFTNPVVKGFYKNHIKSILTR NSITG
Sbjct: 121 ILSLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQ WI+EMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQ NPNFQVGTDFIANNQIPE+DFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDS 360
           NILHKPVLFAEFGKS KYSGADQRDQLFNAVYSAVYSSARGGGAAIGG+FWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGN 420
           FRDGYEVVLSE+PSTANLI+QESRRLIHIR+MYAKLRNIEKWKRA+E++KAQWEA +GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGN 420

Query: 421 KSPGN 425
            SPGN
Sbjct: 421 NSPGN 425

[Close]

BLAST of Lsi04G016900 vs. ExPASy TrEMBL
Match: A0A0A0LDZ5 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G859700 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 5.7e-226
Identity = 387/425 (91.06%), Postives = 408/425 (96.00%), Query Frame = 0


Query: 1   MKLWSSILVLLLPLVL-LAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQR 60
           MKL  SI +LLL L+L  A+ D+GFV T+GQQLILNG+PFYANGFNAYWLMYFASDPSQ 
Sbjct: 1   MKLGISIFILLLLLLLPRAKPDEGFVSTKGQQLILNGSPFYANGFNAYWLMYFASDPSQA 60

Query: 61  TKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKL 120
            KVSSAFQQAVNHGLSIGRTWAF+DGGYSPLQYSPGQYNEKM QGLDFVV+EARKYGIKL
Sbjct: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITG 180
           ILSLVNNY SMGGKKQYVEWARSQGQ+ISSED+FFTNPVVKGFYKNHIKSILTR NSITG
Sbjct: 121 ILSLVNNYASMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQ WI+EMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQGWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQ NPNFQVGTDFIANNQIPE+DFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDS 360
           NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGG+FWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGN 420
           FRDGYEVVLSE+PSTANLI+QESRRLIHIR+MYAKLRNIEKWKRA+E+++AQWEA +GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKRAQWEASRGGN 420

Query: 421 KSPGN 425
            SPGN
Sbjct: 421 NSPGN 425

[Close]

BLAST of Lsi04G016900 vs. ExPASy TrEMBL
Match: A0A6J1BS42 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111005053 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.2e-222
Identity = 381/426 (89.44%), Postives = 408/426 (95.77%), Query Frame = 0


Query: 1   MKLWSSILVLLLPLVLL--AEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQ 60
           MKL S  LV+LL +VL   AEA+DGFVRTRGQQLILNG+PFYANGFNAYWLMYFASDPSQ
Sbjct: 1   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 60

Query: 61  RTKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIK 120
           RTKVSSAFQ+A+NHGLSIGRTWAFSDGGY+PLQYSPGQYNE+M QGLD+V+SEA K+GIK
Sbjct: 61  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 120

Query: 121 LILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSIT 180
           LILSLVNNYE+MGGKKQYVEWARSQGQ+ISSEDDFFTN VVKGFYKNHIKS+L RTNS+T
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 180

Query: 181 GVAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQ 240
           G+AYKDDPTIMAWELMNEARCPSDPSGNTIQ WIREMASYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 240

Query: 241 SKYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDA 300
           S+ QGNPNFQVGT+FIANNQIPELDFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDA
Sbjct: 241 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 300

Query: 301 QNILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMD 360
           QNILHKPVLFAEFGKSTKYSG+DQRDQLFNAVYSAVYSSAR GGAA+GGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 360

Query: 361 SFRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGG 420
           SFRDGYEVVL+EN STANLISQES++LIHIRKMYAKLRNIEK KRA+EIR+AQW+AL GG
Sbjct: 361 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 420

Query: 421 NKSPGN 425
           N SPGN
Sbjct: 421 NNSPGN 426

[Close]

BLAST of Lsi04G016900 vs. ExPASy TrEMBL
Match: A0A6J1FR05 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111446456 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.1e-213
Identity = 370/428 (86.45%), Postives = 399/428 (93.22%), Query Frame = 0


Query: 1   MKLWSSILVLLLPLVLL--AEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQ 60
           M+LWS +LV+L+ +++L  A+ADDGFV TRGQQLILNG+PFYANGFNAYW+MYF SDPSQ
Sbjct: 1   MRLWSPVLVVLVLVLVLVRAKADDGFVSTRGQQLILNGSPFYANGFNAYWVMYFGSDPSQ 60

Query: 61  RTKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIK 120
           R KVSSAF++AV HGLSIGRTWAFSDGG SPLQYSPG YNEKM QGLDF VSEARKYGIK
Sbjct: 61  RNKVSSAFEEAVKHGLSIGRTWAFSDGGDSPLQYSPGSYNEKMFQGLDFAVSEARKYGIK 120

Query: 121 LILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSIT 180
           LILSLVNNYE+MGGKKQYVEWARSQGQSISS+DDFF+NPVVKG YKNHIKSILTR NS T
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQSISSDDDFFSNPVVKGLYKNHIKSILTRINSFT 180

Query: 181 GVAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQ 240
           GVAYKDDPTIMAWELMNE RCPSDPSGNTIQ WIREM SYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GVAYKDDPTIMAWELMNEPRCPSDPSGNTIQAWIREMGSYLKSIDGKHLLEAGLEGFYGQ 240

Query: 241 SKYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDA 300
           S+ QGNPNFQVGTDFIANNQIPE+DFATVHSYPDQWL+GS+ +NQLSFLNTWLN+HIQDA
Sbjct: 241 SRNQGNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLTGSTNQNQLSFLNTWLNEHIQDA 300

Query: 301 QNILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMD 360
           QNILHKPVLFAEFGKSTK S +DQRDQL+NAVYSAVYSSARGGGAA+GGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKNSASDQRDQLYNAVYSAVYSSARGGGAAVGGLFWQLLVEGMD 360

Query: 361 SFRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQW-EALKG 420
           SFRDGYEV+LSEN STANLISQESRRLI IRKMYA+LRNIEK KRA+EI +A+W EALKG
Sbjct: 361 SFRDGYEVILSENLSTANLISQESRRLIRIRKMYARLRNIEKLKRAKEIGRAEWKEALKG 420

Query: 421 G-NKSPGN 425
           G N SPGN
Sbjct: 421 GNNNSPGN 428

[Close]

BLAST of Lsi04G016900 vs. NCBI nr
Match: XP_038897716.1 (mannan endo-1,4-beta-mannosidase 7-like [Benincasa hispida])

HSP 1 Score: 832.8 bits (2150), Expect = 1.3e-237
Identity = 410/424 (96.70%), Postives = 414/424 (97.64%), Query Frame = 0


Query: 1   MKLWSSILVLLLPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRT 60
           MKL  SILVLLLPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRT
Sbjct: 1   MKLSISILVLLLPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRT 60

Query: 61  KVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLI 120
           KVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKM QGLDFVVSEARKYGIKLI
Sbjct: 61  KVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMFQGLDFVVSEARKYGIKLI 120

Query: 121 LSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGV 180
           LSLVNNYESMGGKKQYVEWARSQGQSISSEDDFF+NPVVKGFYKNHIKS+LTR NSITGV
Sbjct: 121 LSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFSNPVVKGFYKNHIKSVLTRINSITGV 180

Query: 181 AYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSK 240
           AYKDDPTIMAWELMNEARCPSDPSGNTIQ WIREMASYLKSIDGKHLLEAGLEGFYGQS+
Sbjct: 181 AYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQSR 240

Query: 241 YQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQN 300
           YQ N NFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQN
Sbjct: 241 YQWNTNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQN 300

Query: 301 ILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSF 360
           ILHKPVL AEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAA+GGLFWQLLVEGMDSF
Sbjct: 301 ILHKPVLLAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAVGGLFWQLLVEGMDSF 360

Query: 361 RDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGNK 420
           RDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGG  
Sbjct: 361 RDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGKN 420

Query: 421 SPGN 425
           SPGN
Sbjct: 421 SPGN 424

[Close]

BLAST of Lsi04G016900 vs. NCBI nr
Match: XP_008466539.2 (PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo] >ADN34185.1 endo-beta-mannanase [Cucumis melo subsp. melo])

HSP 1 Score: 806.2 bits (2081), Expect = 1.3e-229
Identity = 393/425 (92.47%), Postives = 412/425 (96.94%), Query Frame = 0


Query: 1   MKLWSSILVLL-LPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQR 60
           MKL  SI +LL LPLVLLA+ADDGFVRT+GQQLILNGTPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  TKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKL 120
            KVSSAFQQAV+HGLSIGRTWAF+DGGYSPLQYSPGQYNEKM QGLDFVV+EARKYGIKL
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITG 180
           ILSLVNNYESMGGKKQYVEWARSQGQ+ISSED+FFTNPVVKGFYKNHIKSILTR NSITG
Sbjct: 121 ILSLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQ WI+EMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQ NPNFQVGTDFIANNQIPE+DFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDS 360
           NILHKPVLFAEFGKS KYSGADQRDQLFNAVYSAVYSSARGGGAAIGG+FWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGN 420
           FRDGYEVVLSE+PSTANLI+QESRRLIHIR+MYAKLRNIEKWKRA+E++KAQWEA +GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGN 420

Query: 421 KSPGN 425
            SPGN
Sbjct: 421 NSPGN 425

[Close]

BLAST of Lsi04G016900 vs. NCBI nr
Match: XP_004147857.3 (mannan endo-1,4-beta-mannosidase 7 [Cucumis sativus] >KGN60129.2 hypothetical protein Csa_001474 [Cucumis sativus])

HSP 1 Score: 795.4 bits (2053), Expect = 2.4e-226
Identity = 388/425 (91.29%), Postives = 409/425 (96.24%), Query Frame = 0


Query: 1   MKLWSSILVLLLPLVL-LAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQR 60
           MKL  SI +LLL L+L LA+ D+GFV T+GQQLILNG+PFYANGFNAYWLMYFASDPSQ 
Sbjct: 1   MKLGISIFILLLLLLLPLAKPDEGFVSTKGQQLILNGSPFYANGFNAYWLMYFASDPSQA 60

Query: 61  TKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKL 120
            KVSSAFQQAVNHGLSIGRTWAF+DGGYSPLQYSPGQYNEKM QGLDFVV+EARKYGIKL
Sbjct: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITG 180
           ILSLVNNY SMGGKKQYVEWARSQGQ+ISSED+FFTNPVVKGFYKNHIKSILTR NSITG
Sbjct: 121 ILSLVNNYASMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQ WI+EMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQGWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQ NPNFQVGTDFIANNQIPE+DFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDS 360
           NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGG+FWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGN 420
           FRDGYEVVLSE+PSTANLI+QESRRLIHIR+MYAKLRNIEKWKRA+E+++AQWEA +GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKRAQWEASRGGN 420

Query: 421 KSPGN 425
            SPGN
Sbjct: 421 NSPGN 425

[Close]

BLAST of Lsi04G016900 vs. NCBI nr
Match: XP_022132099.1 (mannan endo-1,4-beta-mannosidase 7-like [Momordica charantia])

HSP 1 Score: 781.2 bits (2016), Expect = 4.6e-222
Identity = 381/426 (89.44%), Postives = 408/426 (95.77%), Query Frame = 0


Query: 1   MKLWSSILVLLLPLVLL--AEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQ 60
           MKL S  LV+LL +VL   AEA+DGFVRTRGQQLILNG+PFYANGFNAYWLMYFASDPSQ
Sbjct: 1   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 60

Query: 61  RTKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIK 120
           RTKVSSAFQ+A+NHGLSIGRTWAFSDGGY+PLQYSPGQYNE+M QGLD+V+SEA K+GIK
Sbjct: 61  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 120

Query: 121 LILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSIT 180
           LILSLVNNYE+MGGKKQYVEWARSQGQ+ISSEDDFFTN VVKGFYKNHIKS+L RTNS+T
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 180

Query: 181 GVAYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQ 240
           G+AYKDDPTIMAWELMNEARCPSDPSGNTIQ WIREMASYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 240

Query: 241 SKYQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDA 300
           S+ QGNPNFQVGT+FIANNQIPELDFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDA
Sbjct: 241 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 300

Query: 301 QNILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMD 360
           QNILHKPVLFAEFGKSTKYSG+DQRDQLFNAVYSAVYSSAR GGAA+GGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 360

Query: 361 SFRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGG 420
           SFRDGYEVVL+EN STANLISQES++LIHIRKMYAKLRNIEK KRA+EIR+AQW+AL GG
Sbjct: 361 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 420

Query: 421 NKSPGN 425
           N SPGN
Sbjct: 421 NNSPGN 426

[Close]

BLAST of Lsi04G016900 vs. NCBI nr
Match: XP_023523795.1 (mannan endo-1,4-beta-mannosidase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 758.4 bits (1957), Expect = 3.2e-215
Identity = 369/426 (86.62%), Postives = 400/426 (93.90%), Query Frame = 0


Query: 1   MKLWSSILVLLLPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRT 60
           M+LWS++LV+L+ +++ A+ADDGFV TRGQQLILNG+PFYANGFNAYWLMYF SDPSQR 
Sbjct: 1   MRLWSTVLVVLVLVLVRAKADDGFVSTRGQQLILNGSPFYANGFNAYWLMYFGSDPSQRN 60

Query: 61  KVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLI 120
           KVSSAF++AV HGLSIGRTWAFSDGG SPLQYSPG YNEKM QGLDF VSEARKYGIKLI
Sbjct: 61  KVSSAFEEAVKHGLSIGRTWAFSDGGDSPLQYSPGSYNEKMFQGLDFAVSEARKYGIKLI 120

Query: 121 LSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGV 180
           LSLVNNYE+MGGKKQYVEWAR++GQSISS+DDFF+NPVVKG YKNHIKSILTR NS TGV
Sbjct: 121 LSLVNNYENMGGKKQYVEWARNEGQSISSDDDFFSNPVVKGLYKNHIKSILTRINSFTGV 180

Query: 181 AYKDDPTIMAWELMNEARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSK 240
           AYKDDPTIMAWELMNE RCPSDPSGNTIQ WIREM SYLKSIDGKHLLEAGLEGFYGQS+
Sbjct: 181 AYKDDPTIMAWELMNEPRCPSDPSGNTIQAWIREMGSYLKSIDGKHLLEAGLEGFYGQSR 240

Query: 241 YQGNPNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQN 300
            QGNPNFQVGTDFIANNQIPE+DFATVHSYPDQWL+GS+ +NQLSFLNTWLN+HIQDAQN
Sbjct: 241 NQGNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLTGSTNQNQLSFLNTWLNEHIQDAQN 300

Query: 301 ILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSF 360
           ILHKPVLFAEFGKSTK S +DQRDQL+NAVYSAVYSSARGGGAA+GGLFWQLLVEGMDSF
Sbjct: 301 ILHKPVLFAEFGKSTKNSASDQRDQLYNAVYSAVYSSARGGGAAVGGLFWQLLVEGMDSF 360

Query: 361 RDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQW-EALKGG- 420
           RDGYEV+LSENPSTANLISQESRRLI IRKMYA+LRNIEK KRA+EI +A+W EALKGG 
Sbjct: 361 RDGYEVILSENPSTANLISQESRRLIRIRKMYARLRNIEKLKRAKEIGRAEWEEALKGGN 420

Query: 421 NKSPGN 425
           N SPGN
Sbjct: 421 NNSPGN 426

[Close]

BLAST of Lsi04G016900 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 557.8 bits (1436), Expect = 7.7e-159
Identity = 266/403 (66.00%), Postives = 322/403 (79.90%), Query Frame = 0


Query: 23  GFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIGRTWAF 82
           GFVRT+G Q  LNG P+YANGFNAYWLMY ASDPSQR+K+S+AFQ A  HGL++ RTWAF
Sbjct: 30  GFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAF 89

Query: 83  SDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYVEWARS 142
           SDGGY  LQYSPG YNE M QGLDF ++EAR++GIK+ILS  NNYES GG+KQYV+WARS
Sbjct: 90  SDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARS 149

Query: 143 QGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEARCPSD 202
           +G+ +SSEDDFFT+ +VK FYKNHIK++L R N+ T V YKDDPTIMAWELMNE RCPSD
Sbjct: 150 RGRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSD 209

Query: 203 PSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSKYQG---NPNFQVGTDFIANNQI 262
           PSG  IQ WI EMA+++KS+D  HLLEAGLEGFYGQS  Q    NP  Q GTDFIANN+I
Sbjct: 210 PSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPGQFGTDFIANNRI 269

Query: 263 PELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSG 322
           P +DF TVHSYPD+W   SS ++Q+ FLN WL+ HIQDAQN+LHKP++ AEFGKS K  G
Sbjct: 270 PGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPG 329

Query: 323 --ADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSENPSTANL 382
               QRD +FN VYS +Y SA+ GGAA GGLFWQLLV G+D+F+DGY ++LS++ ST N+
Sbjct: 330 YTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNV 389

Query: 383 ISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGNK 421
           ISQ+SR+L  IRK++A++ N+EKWKRAR     Q +  K G+K
Sbjct: 390 ISQQSRKLTLIRKIFARMINVEKWKRAR----GQGQVGKRGHK 428

[Close]

BLAST of Lsi04G016900 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 440.7 bits (1132), Expect = 1.4e-123
Identity = 201/378 (53.17%), Postives = 270/378 (71.43%), Query Frame = 0


Query: 20  ADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIGRT 79
           + DGFV  +G Q ILNG PFYANGFNAYWL Y A+D + R K++  FQ A  H L+I RT
Sbjct: 27  SSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATDSTTRFKITYVFQNATIHDLTIVRT 86

Query: 80  WAFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYVEW 139
           W F DGGY  LQ +PG Y+EK  QGLDF ++EA++ GIK+I++ VNNY   GG+KQYV+W
Sbjct: 87  WGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRLGIKMIITFVNNYSDFGGRKQYVDW 146

Query: 140 ARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEARC 199
           A++ GQ++SS+DDF+TNP+VK +YKNH+K+++ R N+ T V YKD+PTIM WELMNE +C
Sbjct: 147 AKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNRVNTFTKVEYKDEPTIMGWELMNEPQC 206

Query: 200 PSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSKYQGNPNFQ------VGTDF 259
            +DPSG T+  W+ EMA Y+KS+D KHLL  GLEGFYG S  Q   +        +GTDF
Sbjct: 207 RADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLEGFYGDSSPQRKTSLNPVAANVLGTDF 266

Query: 260 IANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGK 319
           IAN+++  +DFA++HSYPD W      +++L+ L  WL  H++DAQNIL KP++  EFGK
Sbjct: 267 IANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLLRKWLECHLEDAQNILKKPLILGEFGK 326

Query: 320 STKYSG--ADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSEN 379
            T   G    QRD +FNA +  +Y SA  GG A G LFW ++ +GM++F+D   +VLSEN
Sbjct: 327 PTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAAGALFWHVISDGMNNFKDPLSIVLSEN 386

Query: 380 PSTANLISQESRRLIHIR 390
            +T N+I++ESR+L  IR
Sbjct: 387 STTVNIITEESRKLGLIR 404

[Close]

BLAST of Lsi04G016900 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 411.8 bits (1057), Expect = 6.8e-115
Identity = 195/378 (51.59%), Postives = 261/378 (69.05%), Query Frame = 0


Query: 18  AEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIG 77
           A   DGFV   G Q ILNG PFYANGFNAYWL Y A+DP+ R K+++ FQ A + GL+I 
Sbjct: 23  AAPSDGFVSRNGVQFILNGKPFYANGFNAYWLAYEATDPTTRFKITNVFQNATSLGLTIA 82

Query: 78  RTWAFSDGG-YSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQY 137
           RTW F DG  Y  LQ +PG Y+E+  QGLDFV++EA++ GIKLI+ LVNN++  GGKKQY
Sbjct: 83  RTWGFRDGAIYRALQTAPGSYDEQTFQGLDFVIAEAKRIGIKLIILLVNNWDDYGGKKQY 142

Query: 138 VEWARSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNE 197
           V+WARS+G+ +SS DDF+ NPV+K FYKNH+K++L R N+ T VAYKD+P IMAW+LMNE
Sbjct: 143 VDWARSKGEVVSSNDDFYRNPVIKDFYKNHVKTVLNRVNTFTKVAYKDEPAIMAWQLMNE 202

Query: 198 ARCPSDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQSKYQGNPNFQ------VG 257
            RC  D SG T+  WI EMA ++KS+D  HLL  G EGFYG S  +   +        VG
Sbjct: 203 PRCGVDKSGKTLMDWINEMAPFVKSVDPNHLLSTGHEGFYGDSSPERKNSLNPVSANTVG 262

Query: 258 TDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAE 317
            DFIAN+ I  +DFA++H   D W       ++L+F+  WL  HI+DAQNIL KPV+ AE
Sbjct: 263 ADFIANHNIDAIDFASMHCGSDLWFQRLDQNSRLAFIKRWLEGHIEDAQNILKKPVILAE 322

Query: 318 FG---KSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVV 377
           FG    + +Y+ A+ RD +F   Y  +Y+SA+ GG+A G LFW+++ EGM +F     ++
Sbjct: 323 FGLGSDTPRYTLAN-RDGVFTTTYDIIYASAQKGGSAAGALFWEVISEGMSNFAGPSSII 382

Query: 378 LSENPSTANLISQESRRL 386
           LS+  ST N+IS+ +R++
Sbjct: 383 LSDKSSTVNIISEHARKM 399

[Close]

BLAST of Lsi04G016900 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 402.5 bits (1033), Expect = 4.1e-112
Identity = 189/369 (51.22%), Postives = 259/369 (70.19%), Query Frame = 0


Query: 25  VRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQRTKVSSAFQQAVNHGLSIGRTWAFSD 84
           V+ +G Q  LNG PFY NGFN YW+M  A+D S R KV+  FQQA   G+++GRTWAF+D
Sbjct: 48  VQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFND 107

Query: 85  GGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYVEWARSQG 144
           G +  LQ SP  Y+E++ + LDFV+SEARKY I+LILSLVNN+++ GGK QYV+W  + G
Sbjct: 108 GQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASG 167

Query: 145 QSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEARCPSDPS 204
            +++S+DDFFTNP ++ FY++H++++L R N+ T + YK+DPTI AWELMNE RCPSDPS
Sbjct: 168 LNLTSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPS 227

Query: 205 GNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQS---KYQGNPN---FQVGTDFIANNQ 264
           G+ +Q+WI+EMA ++KS+D KHL+E GLEGFYG S   + + NPN    QVGTDFI NNQ
Sbjct: 228 GDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQ 287

Query: 265 IPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYS 324
           +  +DFA+VH YPD W+S +   + L F ++W+  H++DA+  L  PVLF EFG S    
Sbjct: 288 VLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDP 347

Query: 325 GADQ--RDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSENPSTAN 384
           G +   RD + N VY    +S R GGA  G L WQ+  +G +   DGY V L+   + + 
Sbjct: 348 GFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASK 407

Query: 385 LISQESRRL 386
           +IS +S+RL
Sbjct: 408 IISLQSKRL 416

[Close]

BLAST of Lsi04G016900 vs. TAIR 10
Match: AT1G02310.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 391.7 bits (1005), Expect = 7.3e-109
Identity = 192/379 (50.66%), Postives = 256/379 (67.55%), Query Frame = 0


Query: 23  GFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQ--RTKVSSAFQQAVNHGLSIGRTW 82
           GFV   G Q +LNG   Y NGFNAYW+M  A+D +   R  V++A +QA   G+++ R W
Sbjct: 28  GFVGRNGTQFVLNGEQVYLNGFNAYWMMTTAADTASKGRATVTTALRQASAVGMNVARIW 87

Query: 83  AFSDGGYSPLQYSPGQYNEKMLQGLDFVVSEARKYGIKLILSLVNNYESMGGKKQYVEWA 142
            F++G Y PLQ SPG Y+E + +GLDFVV EA ++ IKLI+SLVNN+E  GG+K+YVEWA
Sbjct: 88  GFNEGDYIPLQISPGSYSEDVFKGLDFVVYEAGRFNIKLIISLVNNFEDYGGRKKYVEWA 147

Query: 143 RSQGQSISSEDDFFTNPVVKGFYKNHIKSILTRTNSITGVAYKDDPTIMAWELMNEARCP 202
                 +   D+F+TN  VK FYKNH+K++LTR N+ITG  YKDDPTI +WEL+NE RC 
Sbjct: 148 -----GLDEPDEFYTNSAVKQFYKNHVKTVLTRKNTITGRMYKDDPTIFSWELINEPRCN 207

Query: 203 SDPSGNTIQTWIREMASYLKSIDGKHLLEAGLEGFYGQS---KYQGNPNFQV--GTDFIA 262
              + N +Q W++EMASY+KSID  HLLE GLEGFYG+S   +   NP  +V  GTDFI 
Sbjct: 208 DSTASNILQDWVKEMASYVKSIDSNHLLEIGLEGFYGESIPERTVYNPGGRVLTGTDFIT 267

Query: 263 NNQIPELDFATVHSYPDQW--LSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGK 322
           NNQIP++DFAT+H YPD W  L  S    Q +F++ W+  HI+D  NI+ KP+L  EFGK
Sbjct: 268 NNQIPDIDFATIHIYPDSWLPLQSSRTGEQDTFVDRWIGAHIEDCDNIIKKPLLITEFGK 327

Query: 323 STKYSG--ADQRDQLFNAVYSAVYSSARGGGAAIGGLFWQLLVEGMDSFRDGYEVVLSEN 382
           S+KY G   ++R++ F  VY  +Y SAR GG+  GG+FWQL         DGYEV +   
Sbjct: 328 SSKYPGFSLEKRNKFFQRVYDVIYDSARAGGSCTGGVFWQLTTNRTGLLGDGYEVFMQAG 387

Query: 383 P-STANLISQESRRLIHIR 390
           P +TA LI+ +S +L +++
Sbjct: 388 PNTTAQLIADQSSKLKNLK 401

The following BLAST results are available for this feature:

BLAST of Lsi04G016900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJZ3	1.1e-157	66.00	Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ...	[more]
Q0JKM9	1.4e-144	61.65	Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q6Z310	5.3e-128	57.45	Putative mannan endo-1,4-beta-mannosidase 9 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q8L5J1	2.0e-127	58.49	Mannan endo-1,4-beta-mannosidase 4 OS=Solanum lycopersicum OX=4081 GN=MAN4 PE=1 ...	[more]
Q9FZ03	1.2e-124	54.25	Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 ...	[more]

BLAST of Lsi04G016900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
E5GCI6	6.5e-230	92.47	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S3CRH7	6.5e-230	92.47	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103503926 PE=3 SV...	[more]
A0A0A0LDZ5	5.7e-226	91.06	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G859700 PE=3...	[more]
A0A6J1BS42	2.2e-222	89.44	Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111005053 ...	[more]
A0A6J1FR05	1.1e-213	86.45	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111446456 P...	[more]

BLAST of Lsi04G016900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897716.1	1.3e-237	96.70	mannan endo-1,4-beta-mannosidase 7-like [Benincasa hispida]	[more]
XP_008466539.2	1.3e-229	92.47	PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo] >ADN34185.1 en...	[more]
XP_004147857.3	2.4e-226	91.29	mannan endo-1,4-beta-mannosidase 7 [Cucumis sativus] >KGN60129.2 hypothetical pr...	[more]
XP_022132099.1	4.6e-222	89.44	mannan endo-1,4-beta-mannosidase 7-like [Momordica charantia]	[more]
XP_023523795.1	3.2e-215	86.62	mannan endo-1,4-beta-mannosidase 7-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi04G016900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66460.1	7.7e-159	66.00	Glycosyl hydrolase superfamily protein	[more]
AT3G10890.1	1.4e-123	53.17	Glycosyl hydrolase superfamily protein	[more]
AT3G10900.1	6.8e-115	51.59	Glycosyl hydrolase superfamily protein	[more]
AT5G01930.1	4.1e-112	51.22	Glycosyl hydrolase superfamily protein	[more]
AT1G02310.1	7.3e-109	50.66	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 20..389 e-value: 4.8E-137 score: 458.8
None	No IPR available	PANTHER	PTHR31451:SF43	MANNAN ENDO-1,4-BETA-MANNOSIDASE-LIKE PROTEIN	coord: 13..409
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 91..329 e-value: 4.1E-12 score: 46.1
IPR045053	Mannan endo-1,4-beta-mannosidase-like	PANTHER	PTHR31451	FAMILY NOT NAMED	coord: 13..409
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 24..378

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G016900.1	Lsi04G016900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds