Lsi04G016790 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G016790
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCysteine/Histidine-rich C1 domain family protein, putative
Locationchr04: 24207896 .. 24209989 (+)
RNA-Seq ExpressionLsi04G016790
SyntenyLsi04G016790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGAGCTTGAGCTTGAGGAGTTAGAGGCAGAGGGATCAGAGATGGAGAAGTTGAAGCTTTTGGAGCAGCCACACCCGCACCCATTGACTTACATAGAAGAAAGAACAGACAGCCGAGAAGACATGTTCTGTTGTGTTTGTAAAAAATCATTGTATCCCCCAGCTTTCATTTGTTCCCTTTGCAAATTCTACATTCATCAATCCTGCATTCACCTTCCTCATAAAATCCGCTCCCGTTTCCATCCCCACCATCTTCTTTCCCTCGACACGAGCAACGCCGACCATTGCCATTGTTGCTGGCAGATGCCCTGTGATTGCTTCTACAAGTGCACCCATCGCGGTTGCGAATTCATCGTTGATATCAAATGCACTCTCGCTGACACGAAACGTCCTGGCTTGAACTCCATAGCCAAACATTTCAGCCATTCACATCCTTTGATCCTCGAGAACGAAATCGGTACGAGGAGGCTGGTTGTTTGCCATCTTTGTGGAATGCTGCTTGTTTCTGGTCCTGCTTACTTTTGCTCTAAATGTAATATCCGTTTTCATAAAGACTGTGCCGAACTGCCGCAAGAGATATTGCAACCCAACCAACACCATCACCCCCTCTTCCTTTTTCCCCATGCCAAGCTTCATTCTTTCTGCAATAGCTGCAAAAACGAATGCTCGCAGTTCGTTTACAGCTGTGTTCACTGCAATTTCAATCTCCATGTAATGTGTAGAGCATCTTCAAAGCACAAACACAACTTCACCAGACTGCGGACTAGAATTAGATTTACCTGCCTGTTATGTGGTTGGCCGGGACTGGATTTTCCATGGTTTTGTAGTATCTGCCATCTTTTGGCTCATGAGAAATGCGCTGAATTGCCCCCATCCCTCTTGGTTGTTGGACATGACTGTCCTCTTAATTTCACATATGTTCACCCTTTTGGAGTTCAGACCAAGTTAGCTTGCGATATCTGCAGAAAGAAGGTTGAACCACAGTTCGCAGCATACAACTGTTCCAAATGCAGCTACGTAGTTCATTTGAATTGTGCTGGGAAAAAGTACTTGCGGGGACTAGAACAACATGATGGGCGATTATACACAGGAGGAAGAGGCAGACTAAAATCTTCATCTCAAATCTGCATCAATCCATTAGGAGATGAAGATGCAACATCTAATAATTTTTCAAAGAAAACGTTTGAAGAAGTTGGTAATTTGATTGAGATTCTGCATTCAAATTGTGAACAGGAGTTAATCCTCTGCAAAGAAGAGGTTGACAATGATAAACAATGCCATGGATGTATGCAACGTTTCTCAGTAACTAAGCCATCTTACTCTTACAGTTGTGTTGGATGTGGTTTCTTTCTCCATAAAGATTGTGTTGATTTGCCAATTACTAAGAGACACCCACTTCATAAACATCCTTTAACTCTTATTGCAACACAAGATGTGGCATTTCAGTGCCATGCTTGTCTGCAATTCTGCCATGGCTTTGCCTACCACTGTGAAGAATGTCTCTACACACTTGACATTCGTTGTGTTTTGATCAAAACCAAGAAACTGAAGCATCCAAGTCATGGGCACTTACTGTCTCTTGCACAGAACCATGAGGATCAAACATGTAGGGGATGTGGCCAGAGGAACAAGACGGTGTTCGAATGCGACGATGGCTGCAACAACTTCTGTTTGGACTACAGATGTGCAACTCTACCACAGAAAGTAAGATGCAAATTTGATGGTAGTTTGTTGTTTCTAACTTTCTCTGTGGAAGATGAAACAGGTGAATATTACTGTGATGTATGCGAAGAAGAGAGGAACCCAGCCGGGTGTTTTTATTACTGCAAGACATGTCGATTAGCTGCCCACACTGAGTGCATTCTTGGGGAGTATCCATGGCTCAAGTATGGATCATATGAAACTCACAAGCATCTCTTGGCTCTTGTGACAGAGGGGAAGATGAATTACTCTGATTGTGATCACTGTGGGAAACCTTGTGCCGGAAACTTGGCCTATGAATGCTGCCGCTGCAAGTTCAATGTCCATGCTATTGGGCAGTGTTACCACCAGCAGATACTCAAAGGCAAACTAAGTTTCAAGATCAAGTGA

mRNA sequence

ATGGTTGAGCTTGAGCTTGAGGAGTTAGAGGCAGAGGGATCAGAGATGGAGAAGTTGAAGCTTTTGGAGCAGCCACACCCGCACCCATTGACTTACATAGAAGAAAGAACAGACAGCCGAGAAGACATGTTCTGTTGTGTTTGTAAAAAATCATTGTATCCCCCAGCTTTCATTTGTTCCCTTTGCAAATTCTACATTCATCAATCCTGCATTCACCTTCCTCATAAAATCCGCTCCCGTTTCCATCCCCACCATCTTCTTTCCCTCGACACGAGCAACGCCGACCATTGCCATTGTTGCTGGCAGATGCCCTGTGATTGCTTCTACAAGTGCACCCATCGCGGTTGCGAATTCATCGTTGATATCAAATGCACTCTCGCTGACACGAAACGTCCTGGCTTGAACTCCATAGCCAAACATTTCAGCCATTCACATCCTTTGATCCTCGAGAACGAAATCGGTACGAGGAGGCTGGTTGTTTGCCATCTTTGTGGAATGCTGCTTGTTTCTGGTCCTGCTTACTTTTGCTCTAAATGTAATATCCGTTTTCATAAAGACTGTGCCGAACTGCCGCAAGAGATATTGCAACCCAACCAACACCATCACCCCCTCTTCCTTTTTCCCCATGCCAAGCTTCATTCTTTCTGCAATAGCTGCAAAAACGAATGCTCGCAGTTCGTTTACAGCTGTGTTCACTGCAATTTCAATCTCCATGTAATGTGTAGAGCATCTTCAAAGCACAAACACAACTTCACCAGACTGCGGACTAGAATTAGATTTACCTGCCTGTTATGTGGTTGGCCGGGACTGGATTTTCCATGGTTTTGTAGTATCTGCCATCTTTTGGCTCATGAGAAATGCGCTGAATTGCCCCCATCCCTCTTGGTTGTTGGACATGACTGTCCTCTTAATTTCACATATGTTCACCCTTTTGGAGTTCAGACCAAGTTAGCTTGCGATATCTGCAGAAAGAAGGTTGAACCACAGTTCGCAGCATACAACTGTTCCAAATGCAGCTACGTAGTTCATTTGAATTGTGCTGGGAAAAAGTACTTGCGGGGACTAGAACAACATGATGGGCGATTATACACAGGAGGAAGAGGCAGACTAAAATCTTCATCTCAAATCTGCATCAATCCATTAGGAGATGAAGATGCAACATCTAATAATTTTTCAAAGAAAACGTTTGAAGAAGTTGGTAATTTGATTGAGATTCTGCATTCAAATTGTGAACAGGAGTTAATCCTCTGCAAAGAAGAGGTTGACAATGATAAACAATGCCATGGATGTATGCAACGTTTCTCAGTAACTAAGCCATCTTACTCTTACAGTTGTGTTGGATGTGGTTTCTTTCTCCATAAAGATTGTGTTGATTTGCCAATTACTAAGAGACACCCACTTCATAAACATCCTTTAACTCTTATTGCAACACAAGATGTGGCATTTCAGTGCCATGCTTGTCTGCAATTCTGCCATGGCTTTGCCTACCACTGTGAAGAATGTCTCTACACACTTGACATTCGTTGTGTTTTGATCAAAACCAAGAAACTGAAGCATCCAAGTCATGGGCACTTACTGTCTCTTGCACAGAACCATGAGGATCAAACATGTAGGGGATGTGGCCAGAGGAACAAGACGGTGTTCGAATGCGACGATGGCTGCAACAACTTCTGTTTGGACTACAGATGTGCAACTCTACCACAGAAAGTAAGATGCAAATTTGATGGTAGTTTGTTGTTTCTAACTTTCTCTGTGGAAGATGAAACAGGTGAATATTACTGTGATGTATGCGAAGAAGAGAGGAACCCAGCCGGGTGTTTTTATTACTGCAAGACATGTCGATTAGCTGCCCACACTGAGTGCATTCTTGGGGAGTATCCATGGCTCAAGTATGGATCATATGAAACTCACAAGCATCTCTTGGCTCTTGTGACAGAGGGGAAGATGAATTACTCTGATTGTGATCACTGTGGGAAACCTTGTGCCGGAAACTTGGCCTATGAATGCTGCCGCTGCAAGTTCAATGTCCATGCTATTGGGCAGTGTTACCACCAGCAGATACTCAAAGGCAAACTAAGTTTCAAGATCAAGTGA

Coding sequence (CDS)

ATGGTTGAGCTTGAGCTTGAGGAGTTAGAGGCAGAGGGATCAGAGATGGAGAAGTTGAAGCTTTTGGAGCAGCCACACCCGCACCCATTGACTTACATAGAAGAAAGAACAGACAGCCGAGAAGACATGTTCTGTTGTGTTTGTAAAAAATCATTGTATCCCCCAGCTTTCATTTGTTCCCTTTGCAAATTCTACATTCATCAATCCTGCATTCACCTTCCTCATAAAATCCGCTCCCGTTTCCATCCCCACCATCTTCTTTCCCTCGACACGAGCAACGCCGACCATTGCCATTGTTGCTGGCAGATGCCCTGTGATTGCTTCTACAAGTGCACCCATCGCGGTTGCGAATTCATCGTTGATATCAAATGCACTCTCGCTGACACGAAACGTCCTGGCTTGAACTCCATAGCCAAACATTTCAGCCATTCACATCCTTTGATCCTCGAGAACGAAATCGGTACGAGGAGGCTGGTTGTTTGCCATCTTTGTGGAATGCTGCTTGTTTCTGGTCCTGCTTACTTTTGCTCTAAATGTAATATCCGTTTTCATAAAGACTGTGCCGAACTGCCGCAAGAGATATTGCAACCCAACCAACACCATCACCCCCTCTTCCTTTTTCCCCATGCCAAGCTTCATTCTTTCTGCAATAGCTGCAAAAACGAATGCTCGCAGTTCGTTTACAGCTGTGTTCACTGCAATTTCAATCTCCATGTAATGTGTAGAGCATCTTCAAAGCACAAACACAACTTCACCAGACTGCGGACTAGAATTAGATTTACCTGCCTGTTATGTGGTTGGCCGGGACTGGATTTTCCATGGTTTTGTAGTATCTGCCATCTTTTGGCTCATGAGAAATGCGCTGAATTGCCCCCATCCCTCTTGGTTGTTGGACATGACTGTCCTCTTAATTTCACATATGTTCACCCTTTTGGAGTTCAGACCAAGTTAGCTTGCGATATCTGCAGAAAGAAGGTTGAACCACAGTTCGCAGCATACAACTGTTCCAAATGCAGCTACGTAGTTCATTTGAATTGTGCTGGGAAAAAGTACTTGCGGGGACTAGAACAACATGATGGGCGATTATACACAGGAGGAAGAGGCAGACTAAAATCTTCATCTCAAATCTGCATCAATCCATTAGGAGATGAAGATGCAACATCTAATAATTTTTCAAAGAAAACGTTTGAAGAAGTTGGTAATTTGATTGAGATTCTGCATTCAAATTGTGAACAGGAGTTAATCCTCTGCAAAGAAGAGGTTGACAATGATAAACAATGCCATGGATGTATGCAACGTTTCTCAGTAACTAAGCCATCTTACTCTTACAGTTGTGTTGGATGTGGTTTCTTTCTCCATAAAGATTGTGTTGATTTGCCAATTACTAAGAGACACCCACTTCATAAACATCCTTTAACTCTTATTGCAACACAAGATGTGGCATTTCAGTGCCATGCTTGTCTGCAATTCTGCCATGGCTTTGCCTACCACTGTGAAGAATGTCTCTACACACTTGACATTCGTTGTGTTTTGATCAAAACCAAGAAACTGAAGCATCCAAGTCATGGGCACTTACTGTCTCTTGCACAGAACCATGAGGATCAAACATGTAGGGGATGTGGCCAGAGGAACAAGACGGTGTTCGAATGCGACGATGGCTGCAACAACTTCTGTTTGGACTACAGATGTGCAACTCTACCACAGAAAGTAAGATGCAAATTTGATGGTAGTTTGTTGTTTCTAACTTTCTCTGTGGAAGATGAAACAGGTGAATATTACTGTGATGTATGCGAAGAAGAGAGGAACCCAGCCGGGTGTTTTTATTACTGCAAGACATGTCGATTAGCTGCCCACACTGAGTGCATTCTTGGGGAGTATCCATGGCTCAAGTATGGATCATATGAAACTCACAAGCATCTCTTGGCTCTTGTGACAGAGGGGAAGATGAATTACTCTGATTGTGATCACTGTGGGAAACCTTGTGCCGGAAACTTGGCCTATGAATGCTGCCGCTGCAAGTTCAATGTCCATGCTATTGGGCAGTGTTACCACCAGCAGATACTCAAAGGCAAACTAAGTTTCAAGATCAAGTGA

Protein sequence

MVELELEELEAEGSEMEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLPHKIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLNSIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEILQPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRLRTRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQTKLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRFSVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGFAYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGCNNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCRLAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKFNVHAIGQCYHQQILKGKLSFKIK
Homology
BLAST of Lsi04G016790 vs. ExPASy TrEMBL
Match: A0A0A0LDB0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G857600 PE=4 SV=1)

HSP 1 Score: 1203.7 bits (3113), Expect = 0.0e+00
Identity = 542/683 (79.36%), Postives = 590/683 (86.38%), Query Frame = 0

Query: 16  MEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLPH 75
           M K + LE+PHPHPLTYIEE+T+S+EDMFC +CKK LYPPAFICS CKFY+HQSCI+ P 
Sbjct: 1   MMKWEALEKPHPHPLTYIEEKTESQEDMFCYICKKPLYPPAFICSTCKFYVHQSCIYFPP 60

Query: 76  KIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLN 135
           +I+SRFHP H LSLD SN DHCHCCWQMP DCFY C+H  C FI+DIKC LADTK PGLN
Sbjct: 61  QIQSRFHPQHFLSLDESNTDHCHCCWQMPRDCFYTCSHPYCRFIIDIKCLLADTKTPGLN 120

Query: 136 SIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEIL 195
           SI KHFSHSHP ILE +I T +LVVCH+CGMLL  GPAYFCS+CNIR HK C +LPQEIL
Sbjct: 121 SIGKHFSHSHPFILEKDISTAKLVVCHVCGMLLPFGPAYFCSECNIRCHKACGDLPQEIL 180

Query: 196 QPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRLR 255
           Q NQH+HPLFLFP+AK  SFCNSCKNECSQFVYSCV CNFNLHV CRASS HKHNFTRLR
Sbjct: 181 QLNQHYHPLFLFPYAKPKSFCNSCKNECSQFVYSCVQCNFNLHVTCRASSNHKHNFTRLR 240

Query: 256 TRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQT 315
           T IRF CLLCGW G DFPWFC+ICHLLAHEKCAELPPSLLVVGHDCPLNFTY HPF  Q+
Sbjct: 241 TMIRFKCLLCGWWGHDFPWFCNICHLLAHEKCAELPPSLLVVGHDCPLNFTYSHPFVNQS 300

Query: 316 KLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQ 375
           KLACDICRKKVEPQFAAY+CSKCSY+VHLNCAGKKYL+GL+QHDGRLY   +  LK+S Q
Sbjct: 301 KLACDICRKKVEPQFAAYSCSKCSYIVHLNCAGKKYLKGLQQHDGRLYITKKDTLKTSFQ 360

Query: 376 -ICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRF 435
             CI    D +A  N+FS KT E     ++ LHSNCEQ LIL K+E ++DKQCHGCMQRF
Sbjct: 361 TYCIR---DGEAMPNDFSNKTLE-----LQTLHSNCEQGLILNKDERNDDKQCHGCMQRF 420

Query: 436 SVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGF 495
            +T+PSYSY+C  CGFFLHKDC DLPITKRH LHKH LTL+ATQDVAFQCHACLQ CHGF
Sbjct: 421 PITEPSYSYNCAKCGFFLHKDCADLPITKRHQLHKHSLTLVATQDVAFQCHACLQVCHGF 480

Query: 496 AYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGC 555
           AYHCE+CLYTLD+RC +IKTKKLKHPSH HLLSLAQNH+DQ C+GCGQ NKTVFECDDGC
Sbjct: 481 AYHCEKCLYTLDLRCFMIKTKKLKHPSHRHLLSLAQNHDDQQCKGCGQSNKTVFECDDGC 540

Query: 556 NNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCR 615
           +NF LDYRCATLPQKVRCKFDGSLL+L F VEDETGEYYCDVCEEERNP  CFY+CKTC 
Sbjct: 541 SNFSLDYRCATLPQKVRCKFDGSLLYLRFYVEDETGEYYCDVCEEERNPNVCFYHCKTCH 600

Query: 616 LAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKF 675
           L AH ECILGEYPWLKYGSYETHKH LALVTEGK N+SDCDHCGKPC GNLAYEC RCKF
Sbjct: 601 LMAHPECILGEYPWLKYGSYETHKHPLALVTEGKKNFSDCDHCGKPCTGNLAYECRRCKF 660

Query: 676 NVHAIGQCYHQQILKGKLSFKIK 698
           NVHAIG+CYH QILKGKLSF IK
Sbjct: 661 NVHAIGRCYHDQILKGKLSFMIK 675

BLAST of Lsi04G016790 vs. ExPASy TrEMBL
Match: A0A6J1F8C3 (uncharacterized protein LOC111443068 OS=Cucurbita moschata OX=3662 GN=LOC111443068 PE=4 SV=1)

HSP 1 Score: 1201.8 bits (3108), Expect = 0.0e+00
Identity = 539/683 (78.92%), Postives = 591/683 (86.53%), Query Frame = 0

Query: 15  EMEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLP 74
           EMEK+KLLE+PHPHPL +IEERTDSREDMFCCVCKK L+PPAFICSLCKFYIHQSCIHLP
Sbjct: 17  EMEKMKLLEKPHPHPLCFIEERTDSREDMFCCVCKKELFPPAFICSLCKFYIHQSCIHLP 76

Query: 75  HKIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGL 134
            +I SRFHPHH LSL  +N+DHCHCCWQMP DCFY CTH  C+FIVDIKC LADTKRPGL
Sbjct: 77  PQILSRFHPHHPLSLTETNSDHCHCCWQMPRDCFYICTH--CQFIVDIKCILADTKRPGL 136

Query: 135 NSIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEI 194
           N + KHFSHSHPLIL +EI T +LV CH+CG+LLV GP YFCSKC+IRFHK CAELPQEI
Sbjct: 137 NLLGKHFSHSHPLILHSEITTSKLVACHICGLLLVPGPTYFCSKCSIRFHKACAELPQEI 196

Query: 195 LQPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRL 254
           L+P++HHHPLFL+PHA L  FCN+CKN+CS+FVYSCV C+FNLHVMC ASS HKH+F R 
Sbjct: 197 LKPDRHHHPLFLYPHAPLQPFCNTCKNQCSRFVYSCVECDFNLHVMCLASSHHKHDFIRF 256

Query: 255 RTRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQ 314
           RT I F CLLCGW G  FPWFCSICHLLAHE CAELPPSLLVVGHDCPL FTY HPF   
Sbjct: 257 RTIINFRCLLCGWLGHGFPWFCSICHLLAHENCAELPPSLLVVGHDCPLTFTYSHPFEDH 316

Query: 315 TKLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSS 374
           +KLAC+ICRKKVEPQFAAY+CS+CSYVVHL+CAGKKY+RG  + D               
Sbjct: 317 SKLACEICRKKVEPQFAAYSCSECSYVVHLDCAGKKYMRGQTKRD--------------- 376

Query: 375 QICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRF 434
            + INPLGDE   SNN SKKTFEEVG+ +EILHSNCEQ+LILCKEE D DKQC  CM+RF
Sbjct: 377 SLKINPLGDEAPASNNSSKKTFEEVGS-VEILHSNCEQDLILCKEEGDIDKQCRRCMRRF 436

Query: 435 SVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGF 494
           SV KPSYSYSCV CGFFLHKDC DLP+TKRHPLHKHPLTLI TQDVAFQCHACLQFCHG 
Sbjct: 437 SVAKPSYSYSCVKCGFFLHKDCADLPVTKRHPLHKHPLTLITTQDVAFQCHACLQFCHGH 496

Query: 495 AYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGC 554
           AYHCEECLYTLDIRCVLI+T+KLKHPSH HLLSLAQNHED+ C GCG+ N+ VFECDDGC
Sbjct: 497 AYHCEECLYTLDIRCVLIETRKLKHPSHQHLLSLAQNHEDKICSGCGESNQAVFECDDGC 556

Query: 555 NNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCR 614
           NNF LDYRCATLPQK RC+FDG L+ L+FSVED+TGEYYCDVCEEERNP  CFYYCKTCR
Sbjct: 557 NNFSLDYRCATLPQKARCRFDGGLMDLSFSVEDKTGEYYCDVCEEERNPKVCFYYCKTCR 616

Query: 615 LAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKF 674
           LAAH ECILGEYPWLKYGSYETHKHLL+LV EG+ +YSDC+HCGKPCAGNLAYEC RCKF
Sbjct: 617 LAAHPECILGEYPWLKYGSYETHKHLLSLVAEGRRDYSDCEHCGKPCAGNLAYECRRCKF 676

Query: 675 NVHAIGQCYHQQILKGKLSFKIK 698
           NVHAIG+CYH QI+K KLSFK+K
Sbjct: 677 NVHAIGRCYHDQIIKRKLSFKLK 681

BLAST of Lsi04G016790 vs. ExPASy TrEMBL
Match: A0A5D3D866 (DC1 domain-containing family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00520 PE=4 SV=1)

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 544/683 (79.65%), Postives = 588/683 (86.09%), Query Frame = 0

Query: 16  MEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLPH 75
           M K + LE+PHPHPLTYIEE+TD  EDMFC VCKK LYPPAFICS CKFYIHQSCI+ P 
Sbjct: 1   MMKWEALEKPHPHPLTYIEEKTDGEEDMFCYVCKKPLYPPAFICSTCKFYIHQSCIYFPP 60

Query: 76  KIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLN 135
           +I SRFHP H LSLD SNADHCHCCWQMP DCFY C+H  C FI+DIKC LADTK PGLN
Sbjct: 61  QIHSRFHPQHSLSLDESNADHCHCCWQMPRDCFYTCSHPYCRFIIDIKCLLADTKTPGLN 120

Query: 136 SIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEIL 195
           S+ KHFSHSHPLILE +I T +LVVCHLCGMLL  GPAYFCSKCNIR HK C +LPQEIL
Sbjct: 121 SVGKHFSHSHPLILEKDISTTKLVVCHLCGMLLPFGPAYFCSKCNIRCHKACGDLPQEIL 180

Query: 196 QPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRLR 255
           Q NQH+HPLFLFP AK +SFCNSCKNECSQFVYSCV CNFNLH+ CRASS HKHNFTRLR
Sbjct: 181 QLNQHYHPLFLFPLAKPNSFCNSCKNECSQFVYSCVQCNFNLHLTCRASSNHKHNFTRLR 240

Query: 256 TRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQT 315
           T IRF CLLCGW G DFPWFC+ICHLLAHEKCAELPPSLLVVGHDCPLNFTY HPF  Q+
Sbjct: 241 TMIRFKCLLCGWWGHDFPWFCNICHLLAHEKCAELPPSLLVVGHDCPLNFTYSHPFVNQS 300

Query: 316 KLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQ 375
           KLACDICRKKVEPQFAAY+C KCSY+VHLNCAGKKYL+GL QHDGRL+   +  LK+S Q
Sbjct: 301 KLACDICRKKVEPQFAAYSCFKCSYIVHLNCAGKKYLKGLRQHDGRLHITKKDTLKTSFQ 360

Query: 376 -ICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRF 435
             CI    DE+A  N+FS KT E     +E LHSNCEQELIL  +E ++DKQCHGCMQRF
Sbjct: 361 TYCIR---DEEAMPNDFSNKTLE-----LETLHSNCEQELILNIDEPNDDKQCHGCMQRF 420

Query: 436 SVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGF 495
            +++PSY Y+C  CGFFLHKDC DLPITKRH LHKH LTL+ATQDVAFQCHACLQ CHGF
Sbjct: 421 PISEPSYCYNCAKCGFFLHKDCADLPITKRHQLHKHSLTLVATQDVAFQCHACLQVCHGF 480

Query: 496 AYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGC 555
           AYHCEECLYTLD+RC +IKTKKLKHPSH HLLSLAQN++DQ C+GCGQ NKTVFECDDGC
Sbjct: 481 AYHCEECLYTLDLRCFMIKTKKLKHPSHRHLLSLAQNNDDQQCKGCGQSNKTVFECDDGC 540

Query: 556 NNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCR 615
            NF LDYRCATLPQKVRCKFDGSLL+L FSVEDETGEYYCDVCEEERNP  CFYYCKTC 
Sbjct: 541 -NFSLDYRCATLPQKVRCKFDGSLLYLRFSVEDETGEYYCDVCEEERNPNVCFYYCKTCH 600

Query: 616 LAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKF 675
           L AH +CILGEYPWLKYGSYETHKH LALVTEGKMN++DCDHCGKPC GNLAYEC RCKF
Sbjct: 601 LMAHPDCILGEYPWLKYGSYETHKHPLALVTEGKMNFADCDHCGKPCTGNLAYECRRCKF 660

Query: 676 NVHAIGQCYHQQILKGKLSFKIK 698
           NVHAIG+CY+ QILKGKLSF IK
Sbjct: 661 NVHAIGRCYNDQILKGKLSFMIK 674

BLAST of Lsi04G016790 vs. ExPASy TrEMBL
Match: A0A1S3CRK2 (uncharacterized protein LOC103503938 OS=Cucumis melo OX=3656 GN=LOC103503938 PE=4 SV=1)

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 544/683 (79.65%), Postives = 588/683 (86.09%), Query Frame = 0

Query: 16  MEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLPH 75
           M K + LE+PHPHPLTYIEE+TD  EDMFC VCKK LYPPAFICS CKFYIHQSCI+ P 
Sbjct: 1   MMKWEALEKPHPHPLTYIEEKTDGEEDMFCYVCKKPLYPPAFICSTCKFYIHQSCIYFPP 60

Query: 76  KIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLN 135
           +I SRFHP H LSLD SNADHCHCCWQMP DCFY C+H  C FI+DIKC LADTK PGLN
Sbjct: 61  QIHSRFHPQHSLSLDESNADHCHCCWQMPRDCFYTCSHPYCRFIIDIKCLLADTKTPGLN 120

Query: 136 SIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEIL 195
           S+ KHFSHSHPLILE +I T +LVVCHLCGMLL  GPAYFCSKCNIR HK C +LPQEIL
Sbjct: 121 SVGKHFSHSHPLILEKDISTTKLVVCHLCGMLLPFGPAYFCSKCNIRCHKACGDLPQEIL 180

Query: 196 QPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRLR 255
           Q NQH+HPLFLFP AK +SFCNSCKNECSQFVYSCV CNFNLH+ CRASS HKHNFTRLR
Sbjct: 181 QLNQHYHPLFLFPLAKPNSFCNSCKNECSQFVYSCVQCNFNLHLTCRASSNHKHNFTRLR 240

Query: 256 TRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQT 315
           T IRF CLLCGW G DFPWFC+ICHLLAHEKCAELPPSLLVVGHDCPLNFTY HPF  Q+
Sbjct: 241 TMIRFKCLLCGWWGHDFPWFCNICHLLAHEKCAELPPSLLVVGHDCPLNFTYSHPFVNQS 300

Query: 316 KLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQ 375
           KLACDICRKKVEPQFAAY+C KCSY+VHLNCAGKKYL+GL QHDGRL+   +  LK+S Q
Sbjct: 301 KLACDICRKKVEPQFAAYSCFKCSYIVHLNCAGKKYLKGLRQHDGRLHITKKDTLKTSFQ 360

Query: 376 -ICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRF 435
             CI    DE+A  N+FS KT E     +E LHSNCEQELIL  +E ++DKQCHGCMQRF
Sbjct: 361 TYCIR---DEEAMPNDFSNKTLE-----LETLHSNCEQELILNIDEPNDDKQCHGCMQRF 420

Query: 436 SVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGF 495
            +++PSY Y+C  CGFFLHKDC DLPITKRH LHKH LTL+ATQDVAFQCHACLQ CHGF
Sbjct: 421 PISEPSYCYNCAKCGFFLHKDCADLPITKRHQLHKHSLTLVATQDVAFQCHACLQVCHGF 480

Query: 496 AYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGC 555
           AYHCEECLYTLD+RC +IKTKKLKHPSH HLLSLAQN++DQ C+GCGQ NKTVFECDDGC
Sbjct: 481 AYHCEECLYTLDLRCFMIKTKKLKHPSHRHLLSLAQNNDDQQCKGCGQSNKTVFECDDGC 540

Query: 556 NNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCR 615
            NF LDYRCATLPQKVRCKFDGSLL+L FSVEDETGEYYCDVCEEERNP  CFYYCKTC 
Sbjct: 541 -NFSLDYRCATLPQKVRCKFDGSLLYLRFSVEDETGEYYCDVCEEERNPNVCFYYCKTCH 600

Query: 616 LAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKF 675
           L AH +CILGEYPWLKYGSYETHKH LALVTEGKMN++DCDHCGKPC GNLAYEC RCKF
Sbjct: 601 LMAHPDCILGEYPWLKYGSYETHKHPLALVTEGKMNFADCDHCGKPCTGNLAYECRRCKF 660

Query: 676 NVHAIGQCYHQQILKGKLSFKIK 698
           NVHAIG+CY+ QILKGKLSF IK
Sbjct: 661 NVHAIGRCYNDQILKGKLSFMIK 674

BLAST of Lsi04G016790 vs. ExPASy TrEMBL
Match: A0A6J1IGZ8 (uncharacterized protein LOC111475038 OS=Cucurbita maxima OX=3661 GN=LOC111475038 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 8.9e-277
Identity = 434/553 (78.48%), Postives = 472/553 (85.35%), Query Frame = 0

Query: 145 HPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEILQPNQHHHPL 204
           HPLIL +EI T +LV CH+CG+LLVSGP YFCSKC+IRFHK CAELPQEIL+P++HHHPL
Sbjct: 1   HPLILHSEITTSKLVACHICGLLLVSGPTYFCSKCSIRFHKACAELPQEILKPDRHHHPL 60

Query: 205 FLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRLRTRIRFTCLL 264
           FL+PHA L  FCN+CKN+CS+FVYSCV C+FNLHVMC ASS  KHNF   RT I F CLL
Sbjct: 61  FLYPHAPLQPFCNTCKNQCSRFVYSCVECDFNLHVMCLASSHRKHNFIIFRTIINFKCLL 120

Query: 265 CGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQTKLACDICRK 324
           CGW G  FPWFCSICHLLAHE CAELPPSLLVVGHDCPL FTY HPF   +KLAC ICRK
Sbjct: 121 CGWWGHGFPWFCSICHLLAHENCAELPPSLLVVGHDCPLTFTYSHPFEDHSKLACAICRK 180

Query: 325 KVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQICINPLGDE 384
           KVEPQFAAY+CS+CSYVVHLNCAGKKY+RG  + D                + INPLGDE
Sbjct: 181 KVEPQFAAYSCSECSYVVHLNCAGKKYMRGQTKRD---------------SLKINPLGDE 240

Query: 385 DATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRFSVTKPSYSYS 444
              SNN SKKTFEEVG+ +EILHSNCEQ+LILCKEE D DKQC  CMQRFSV KPS+SYS
Sbjct: 241 APASNNSSKKTFEEVGS-VEILHSNCEQDLILCKEEGDIDKQCRRCMQRFSVAKPSHSYS 300

Query: 445 CVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGFAYHCEECLYT 504
           CV CGFFLHKDC DLP+TKRHPLHKHPLTLI TQDVAFQCHACLQFCHG AYHCEECLYT
Sbjct: 301 CVKCGFFLHKDCADLPVTKRHPLHKHPLTLITTQDVAFQCHACLQFCHGHAYHCEECLYT 360

Query: 505 LDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGCNNFCLDYRCA 564
           LDIRCVLI+T+KLKHPSH HLLSLAQNHED+ C GCG+ N+ VFECDDGCNNF LDYRCA
Sbjct: 361 LDIRCVLIETRKLKHPSHQHLLSLAQNHEDKICSGCGESNQAVFECDDGCNNFSLDYRCA 420

Query: 565 TLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCRLAAHTECILG 624
           TLPQK RC+FDG L+ L+FSVEDETGEYYCDVCEEERNP  CFYYCKTCRLAAH ECILG
Sbjct: 421 TLPQKARCRFDGGLMDLSFSVEDETGEYYCDVCEEERNPKVCFYYCKTCRLAAHPECILG 480

Query: 625 EYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKFNVHAIGQCYH 684
           EYPWLKYGSYETHKHLL+LV E K +YSDC+HCGKPCAGNLAYEC RCKFNVHAIG+CYH
Sbjct: 481 EYPWLKYGSYETHKHLLSLVAERKRDYSDCEHCGKPCAGNLAYECRRCKFNVHAIGRCYH 537

Query: 685 QQILKGKLSFKIK 698
            QI+K KLSFK+K
Sbjct: 541 DQIIKRKLSFKLK 537

BLAST of Lsi04G016790 vs. NCBI nr
Match: XP_038898645.1 (uncharacterized protein LOC120086189 [Benincasa hispida])

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 566/682 (82.99%), Postives = 606/682 (88.86%), Query Frame = 0

Query: 16  MEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLPH 75
           MEKLKLLEQPH HPLTYIEERTDSRE+MFCCVCKK+LYPPAF+CS CKFYIHQSCIHLP 
Sbjct: 1   MEKLKLLEQPHSHPLTYIEERTDSREEMFCCVCKKALYPPAFVCSHCKFYIHQSCIHLPP 60

Query: 76  KIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLN 135
           +IRSRFHP H LSL  +N+DHC+CCWQMP DCFY C+   C FI+DIKC L   + PGLN
Sbjct: 61  QIRSRFHPQHSLSLTETNSDHCYCCWQMPRDCFYTCSQTYCRFIIDIKCILTIARSPGLN 120

Query: 136 SIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEIL 195
           SI KHFSHSHPLILE E+ TR+LVVCHLCGMLLVSGPAYFCSKCNIRFHK CAELPQEIL
Sbjct: 121 SIGKHFSHSHPLILEKELATRKLVVCHLCGMLLVSGPAYFCSKCNIRFHKGCAELPQEIL 180

Query: 196 QPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRLR 255
           QPNQHHHPLFLFPHAK HSFCNSCKN+CS+FVYSCVHCNF+LHV CR SS HKHNFTRLR
Sbjct: 181 QPNQHHHPLFLFPHAKPHSFCNSCKNQCSEFVYSCVHCNFHLHVTCRVSSNHKHNFTRLR 240

Query: 256 TRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQT 315
           T I F CLLCG  G DFP FCSICHLLAHEKCA LPPSLLV+GHDCPLNFTY HPF  + 
Sbjct: 241 TIISFKCLLCGSWGHDFPLFCSICHLLAHEKCAALPPSLLVIGHDCPLNFTYSHPFEDKD 300

Query: 316 KLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQ 375
           KLACDICRKKVEP+FAAY+CSKCSY+VHLNCA +KYL+GL+QHDGRLY   R +LK+S Q
Sbjct: 301 KLACDICRKKVEPEFAAYSCSKCSYIVHLNCAAEKYLQGLQQHDGRLYISSRSKLKTSFQ 360

Query: 376 ICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRFS 435
             INPL DEDATS + S KT E     +EILHSNCEQELIL KEE +NDKQC+GCMQ +S
Sbjct: 361 TYINPLKDEDATSKSSSNKTLE-----VEILHSNCEQELILYKEEGNNDKQCYGCMQCYS 420

Query: 436 VTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGFA 495
           VT PSYSYSCV CGFFLHKDC DL IT++HPLHKHPLTLI TQDVAFQCHACLQFCHG+A
Sbjct: 421 VTIPSYSYSCVKCGFFLHKDCADLLITRKHPLHKHPLTLITTQDVAFQCHACLQFCHGYA 480

Query: 496 YHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGCN 555
           YHCEECLYTLD+RCVL+KTKKLKHPSH HLLSLAQNH+DQ CRGCGQ NKTVFECDDGC 
Sbjct: 481 YHCEECLYTLDLRCVLLKTKKLKHPSHRHLLSLAQNHDDQQCRGCGQTNKTVFECDDGCK 540

Query: 556 NFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCRL 615
           +F LD+RCATLPQK RCKFDGSLL LTFSVEDETGEYYCDVCEEERNP+ CFYYCKTCRL
Sbjct: 541 DFSLDFRCATLPQKARCKFDGSLLDLTFSVEDETGEYYCDVCEEERNPSVCFYYCKTCRL 600

Query: 616 AAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKFN 675
           AAH ECILGEYPWLKYGSYETHKH LALVTEGKMNYSDCDHCGKPCAGNLAYEC RCKFN
Sbjct: 601 AAHPECILGEYPWLKYGSYETHKHPLALVTEGKMNYSDCDHCGKPCAGNLAYECRRCKFN 660

Query: 676 VHAIGQCYHQQILKGKLSFKIK 698
           VHAIG CYH+QILKGKLSFKIK
Sbjct: 661 VHAIGHCYHEQILKGKLSFKIK 677

BLAST of Lsi04G016790 vs. NCBI nr
Match: XP_023536436.1 (uncharacterized protein LOC111797614 [Cucurbita pepo subsp. pepo] >XP_023536438.1 uncharacterized protein LOC111797614 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 544/696 (78.16%), Postives = 600/696 (86.21%), Query Frame = 0

Query: 2   VELELEELEAEGSEMEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSL 61
           VE E EE +AEGSEMEK++LLE+PHPHPL +IEERTDSREDMFCCVCKK L+PPAFICSL
Sbjct: 21  VEEEEEEQKAEGSEMEKMQLLEKPHPHPLCFIEERTDSREDMFCCVCKKELFPPAFICSL 80

Query: 62  CKFYIHQSCIHLPHKIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVD 121
           CKFYIHQSCIHLP +I SRFHPHH LSL  +N+DHCHCCWQMP DCFY CTH  C+FIVD
Sbjct: 81  CKFYIHQSCIHLPPQILSRFHPHHPLSLTETNSDHCHCCWQMPRDCFYICTH--CQFIVD 140

Query: 122 IKCTLADTKRPGLNSIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNI 181
           IKC LADTKRPGLN + KHFSHSHPLIL ++I T +LV CH+CG+LLV GP YFCSKC+I
Sbjct: 141 IKCILADTKRPGLNLLGKHFSHSHPLILHSQITTSKLVACHICGLLLVPGPTYFCSKCSI 200

Query: 182 RFHKDCAELPQEILQPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMC 241
            FHK CAELPQEIL+P++HHHPLFL+PHA L  FCN+CK++CS+FVYSCV C+FNLHVMC
Sbjct: 201 GFHKACAELPQEILEPDRHHHPLFLYPHAPLQPFCNTCKSQCSRFVYSCVECDFNLHVMC 260

Query: 242 RASSKHKHNFTRLRTRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDC 301
            ASS HKH+F R RT I F CLLCGWPG  FPWFCSICHLLAHE CAELPPSLLVVGHDC
Sbjct: 261 LASSHHKHDFIRFRTIINFRCLLCGWPGHGFPWFCSICHLLAHENCAELPPSLLVVGHDC 320

Query: 302 PLNFTYVHPFGVQTKLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGR 361
           PL FTY HPF   +KLAC+ICRKKVEPQFAAY+CS+CSYVVHL+CAGKKY+RG  + D  
Sbjct: 321 PLTFTYSHPFEDNSKLACEICRKKVEPQFAAYSCSECSYVVHLDCAGKKYMRGQTKRD-- 380

Query: 362 LYTGGRGRLKSSSQICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEV 421
                         + +N LGDE   SNN SKKTFEEVG+ +EILHSNCEQ+LILCKEE 
Sbjct: 381 -------------SLKLNLLGDEAPASNNSSKKTFEEVGS-VEILHSNCEQDLILCKEEG 440

Query: 422 DNDKQCHGCMQRFSVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVA 481
           D DKQC  CMQRF+V KPSYSYSCV CGFFLHKDC DLP+TKRHPLHKHPLTLI TQDVA
Sbjct: 441 DIDKQCRRCMQRFAVAKPSYSYSCVKCGFFLHKDCADLPVTKRHPLHKHPLTLITTQDVA 500

Query: 482 FQCHACLQFCHGFAYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCG 541
           FQCHACLQFCHG AYHCEECLYTLDIRCVLI+T+KLKHPSH HLLSLAQNHED+ C GCG
Sbjct: 501 FQCHACLQFCHGHAYHCEECLYTLDIRCVLIETRKLKHPSHQHLLSLAQNHEDKICSGCG 560

Query: 542 QRNKTVFECDDGCNNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEER 601
           + N+ VFECDDGCNNF LDYRCATLPQK RC+FDG L+ L+FSVED+TGEYYCDVCEEER
Sbjct: 561 ESNQAVFECDDGCNNFSLDYRCATLPQKARCRFDGGLMDLSFSVEDKTGEYYCDVCEEER 620

Query: 602 NPAGCFYYCKTCRLAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPC 661
           NP  CFYYCKTCRLAAH ECILGEYPWLKYGSYETHKHLL+LV EGK +YSDC+HCGKPC
Sbjct: 621 NPKVCFYYCKTCRLAAHPECILGEYPWLKYGSYETHKHLLSLVAEGKRDYSDCEHCGKPC 680

Query: 662 AGNLAYECCRCKFNVHAIGQCYHQQILKGKLSFKIK 698
           AGNLAYEC RCKFNVHAIG+CYH QI+K KLSFK+K
Sbjct: 681 AGNLAYECRRCKFNVHAIGRCYHDQIIKRKLSFKLK 698

BLAST of Lsi04G016790 vs. NCBI nr
Match: XP_004147882.1 (uncharacterized protein LOC101207273 [Cucumis sativus] >KGN59970.1 hypothetical protein Csa_001356 [Cucumis sativus])

HSP 1 Score: 1203.7 bits (3113), Expect = 0.0e+00
Identity = 542/683 (79.36%), Postives = 590/683 (86.38%), Query Frame = 0

Query: 16  MEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLPH 75
           M K + LE+PHPHPLTYIEE+T+S+EDMFC +CKK LYPPAFICS CKFY+HQSCI+ P 
Sbjct: 1   MMKWEALEKPHPHPLTYIEEKTESQEDMFCYICKKPLYPPAFICSTCKFYVHQSCIYFPP 60

Query: 76  KIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLN 135
           +I+SRFHP H LSLD SN DHCHCCWQMP DCFY C+H  C FI+DIKC LADTK PGLN
Sbjct: 61  QIQSRFHPQHFLSLDESNTDHCHCCWQMPRDCFYTCSHPYCRFIIDIKCLLADTKTPGLN 120

Query: 136 SIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEIL 195
           SI KHFSHSHP ILE +I T +LVVCH+CGMLL  GPAYFCS+CNIR HK C +LPQEIL
Sbjct: 121 SIGKHFSHSHPFILEKDISTAKLVVCHVCGMLLPFGPAYFCSECNIRCHKACGDLPQEIL 180

Query: 196 QPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRLR 255
           Q NQH+HPLFLFP+AK  SFCNSCKNECSQFVYSCV CNFNLHV CRASS HKHNFTRLR
Sbjct: 181 QLNQHYHPLFLFPYAKPKSFCNSCKNECSQFVYSCVQCNFNLHVTCRASSNHKHNFTRLR 240

Query: 256 TRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQT 315
           T IRF CLLCGW G DFPWFC+ICHLLAHEKCAELPPSLLVVGHDCPLNFTY HPF  Q+
Sbjct: 241 TMIRFKCLLCGWWGHDFPWFCNICHLLAHEKCAELPPSLLVVGHDCPLNFTYSHPFVNQS 300

Query: 316 KLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQ 375
           KLACDICRKKVEPQFAAY+CSKCSY+VHLNCAGKKYL+GL+QHDGRLY   +  LK+S Q
Sbjct: 301 KLACDICRKKVEPQFAAYSCSKCSYIVHLNCAGKKYLKGLQQHDGRLYITKKDTLKTSFQ 360

Query: 376 -ICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRF 435
             CI    D +A  N+FS KT E     ++ LHSNCEQ LIL K+E ++DKQCHGCMQRF
Sbjct: 361 TYCIR---DGEAMPNDFSNKTLE-----LQTLHSNCEQGLILNKDERNDDKQCHGCMQRF 420

Query: 436 SVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGF 495
            +T+PSYSY+C  CGFFLHKDC DLPITKRH LHKH LTL+ATQDVAFQCHACLQ CHGF
Sbjct: 421 PITEPSYSYNCAKCGFFLHKDCADLPITKRHQLHKHSLTLVATQDVAFQCHACLQVCHGF 480

Query: 496 AYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGC 555
           AYHCE+CLYTLD+RC +IKTKKLKHPSH HLLSLAQNH+DQ C+GCGQ NKTVFECDDGC
Sbjct: 481 AYHCEKCLYTLDLRCFMIKTKKLKHPSHRHLLSLAQNHDDQQCKGCGQSNKTVFECDDGC 540

Query: 556 NNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCR 615
           +NF LDYRCATLPQKVRCKFDGSLL+L F VEDETGEYYCDVCEEERNP  CFY+CKTC 
Sbjct: 541 SNFSLDYRCATLPQKVRCKFDGSLLYLRFYVEDETGEYYCDVCEEERNPNVCFYHCKTCH 600

Query: 616 LAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKF 675
           L AH ECILGEYPWLKYGSYETHKH LALVTEGK N+SDCDHCGKPC GNLAYEC RCKF
Sbjct: 601 LMAHPECILGEYPWLKYGSYETHKHPLALVTEGKKNFSDCDHCGKPCTGNLAYECRRCKF 660

Query: 676 NVHAIGQCYHQQILKGKLSFKIK 698
           NVHAIG+CYH QILKGKLSF IK
Sbjct: 661 NVHAIGRCYHDQILKGKLSFMIK 675

BLAST of Lsi04G016790 vs. NCBI nr
Match: XP_022936454.1 (uncharacterized protein LOC111443068 [Cucurbita moschata])

HSP 1 Score: 1201.8 bits (3108), Expect = 0.0e+00
Identity = 539/683 (78.92%), Postives = 591/683 (86.53%), Query Frame = 0

Query: 15  EMEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLP 74
           EMEK+KLLE+PHPHPL +IEERTDSREDMFCCVCKK L+PPAFICSLCKFYIHQSCIHLP
Sbjct: 17  EMEKMKLLEKPHPHPLCFIEERTDSREDMFCCVCKKELFPPAFICSLCKFYIHQSCIHLP 76

Query: 75  HKIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGL 134
            +I SRFHPHH LSL  +N+DHCHCCWQMP DCFY CTH  C+FIVDIKC LADTKRPGL
Sbjct: 77  PQILSRFHPHHPLSLTETNSDHCHCCWQMPRDCFYICTH--CQFIVDIKCILADTKRPGL 136

Query: 135 NSIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEI 194
           N + KHFSHSHPLIL +EI T +LV CH+CG+LLV GP YFCSKC+IRFHK CAELPQEI
Sbjct: 137 NLLGKHFSHSHPLILHSEITTSKLVACHICGLLLVPGPTYFCSKCSIRFHKACAELPQEI 196

Query: 195 LQPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRL 254
           L+P++HHHPLFL+PHA L  FCN+CKN+CS+FVYSCV C+FNLHVMC ASS HKH+F R 
Sbjct: 197 LKPDRHHHPLFLYPHAPLQPFCNTCKNQCSRFVYSCVECDFNLHVMCLASSHHKHDFIRF 256

Query: 255 RTRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQ 314
           RT I F CLLCGW G  FPWFCSICHLLAHE CAELPPSLLVVGHDCPL FTY HPF   
Sbjct: 257 RTIINFRCLLCGWLGHGFPWFCSICHLLAHENCAELPPSLLVVGHDCPLTFTYSHPFEDH 316

Query: 315 TKLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSS 374
           +KLAC+ICRKKVEPQFAAY+CS+CSYVVHL+CAGKKY+RG  + D               
Sbjct: 317 SKLACEICRKKVEPQFAAYSCSECSYVVHLDCAGKKYMRGQTKRD--------------- 376

Query: 375 QICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRF 434
            + INPLGDE   SNN SKKTFEEVG+ +EILHSNCEQ+LILCKEE D DKQC  CM+RF
Sbjct: 377 SLKINPLGDEAPASNNSSKKTFEEVGS-VEILHSNCEQDLILCKEEGDIDKQCRRCMRRF 436

Query: 435 SVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGF 494
           SV KPSYSYSCV CGFFLHKDC DLP+TKRHPLHKHPLTLI TQDVAFQCHACLQFCHG 
Sbjct: 437 SVAKPSYSYSCVKCGFFLHKDCADLPVTKRHPLHKHPLTLITTQDVAFQCHACLQFCHGH 496

Query: 495 AYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGC 554
           AYHCEECLYTLDIRCVLI+T+KLKHPSH HLLSLAQNHED+ C GCG+ N+ VFECDDGC
Sbjct: 497 AYHCEECLYTLDIRCVLIETRKLKHPSHQHLLSLAQNHEDKICSGCGESNQAVFECDDGC 556

Query: 555 NNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCR 614
           NNF LDYRCATLPQK RC+FDG L+ L+FSVED+TGEYYCDVCEEERNP  CFYYCKTCR
Sbjct: 557 NNFSLDYRCATLPQKARCRFDGGLMDLSFSVEDKTGEYYCDVCEEERNPKVCFYYCKTCR 616

Query: 615 LAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKF 674
           LAAH ECILGEYPWLKYGSYETHKHLL+LV EG+ +YSDC+HCGKPCAGNLAYEC RCKF
Sbjct: 617 LAAHPECILGEYPWLKYGSYETHKHLLSLVAEGRRDYSDCEHCGKPCAGNLAYECRRCKF 676

Query: 675 NVHAIGQCYHQQILKGKLSFKIK 698
           NVHAIG+CYH QI+K KLSFK+K
Sbjct: 677 NVHAIGRCYHDQIIKRKLSFKLK 681

BLAST of Lsi04G016790 vs. NCBI nr
Match: KAG7024193.1 (hypothetical protein SDJN02_13007, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 539/683 (78.92%), Postives = 591/683 (86.53%), Query Frame = 0

Query: 15  EMEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLP 74
           EMEK+KLLE+PHPHPL +IEERTDSREDMFCCVCKK L+PPAFICSLCKFYIHQSCIHLP
Sbjct: 17  EMEKMKLLEKPHPHPLCFIEERTDSREDMFCCVCKKELFPPAFICSLCKFYIHQSCIHLP 76

Query: 75  HKIRSRFHPHHLLSLDTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGL 134
            +I SRFHPHH LSL  +N+DHCHCCWQMP DCFY CTH  C+FIVDIKC LADTKRPGL
Sbjct: 77  PQILSRFHPHHPLSLTETNSDHCHCCWQMPRDCFYICTH--CQFIVDIKCILADTKRPGL 136

Query: 135 NSIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEI 194
           N + KHFSHSHPLIL +EI T +LV CH+CG+LLV GP YFCSKC+IRFHK CAELPQEI
Sbjct: 137 NLLGKHFSHSHPLILHSEITTSKLVACHICGLLLVPGPTYFCSKCSIRFHKACAELPQEI 196

Query: 195 LQPNQHHHPLFLFPHAKLHSFCNSCKNECSQFVYSCVHCNFNLHVMCRASSKHKHNFTRL 254
           L+P++HHHPLFL+PHA L  FCN+CKN+CS+FVYSCV C+FNLHVMC ASS HKH+F R 
Sbjct: 197 LKPDRHHHPLFLYPHAPLQPFCNTCKNQCSRFVYSCVECDFNLHVMCLASSHHKHDFIRF 256

Query: 255 RTRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQ 314
           RT I F CLLCGW G  FPWFCSICHLLAHE CAELPPSLLVVGHDCPL FTY HPF   
Sbjct: 257 RTIINFRCLLCGWLGHGFPWFCSICHLLAHENCAELPPSLLVVGHDCPLTFTYSHPFEDH 316

Query: 315 TKLACDICRKKVEPQFAAYNCSKCSYVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSS 374
           +KLAC+ICRKKVEPQFAAY+CS+CSYVVHL+CAGKKY+RG  + D               
Sbjct: 317 SKLACEICRKKVEPQFAAYSCSECSYVVHLDCAGKKYMRGQTKRD--------------- 376

Query: 375 QICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRF 434
            + INPLGDE   SNN SKKTFEEVG+ +EILHSNCEQ+LILCKEE D DKQC  CMQRF
Sbjct: 377 SLKINPLGDEAPASNNSSKKTFEEVGS-VEILHSNCEQDLILCKEEGDIDKQCRRCMQRF 436

Query: 435 SVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGF 494
           SV KPSYSYSCV CGFFLHKDC DLP+TKRHPLHKHPLTLI T+DVAFQCHACLQFCHG 
Sbjct: 437 SVAKPSYSYSCVKCGFFLHKDCADLPVTKRHPLHKHPLTLITTRDVAFQCHACLQFCHGH 496

Query: 495 AYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGC 554
           AYHCEECLYTLDIRCVLI+T+KLKHPSH HLLSLAQNHED+ C GCG+ N+ VFECDDGC
Sbjct: 497 AYHCEECLYTLDIRCVLIETRKLKHPSHQHLLSLAQNHEDKICSGCGESNQAVFECDDGC 556

Query: 555 NNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCR 614
           NNF LDYRCATLPQK RC+FDG L+ L+FSVED+TGEYYCDVCEEERNP  CFYYCKTCR
Sbjct: 557 NNFSLDYRCATLPQKARCRFDGGLMDLSFSVEDKTGEYYCDVCEEERNPKVCFYYCKTCR 616

Query: 615 LAAHTECILGEYPWLKYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKF 674
           LAAH ECILGEYPWLKYGSYETHKHLL+LV EG+ +YSDC+HCGKPCAGNLAYEC RCKF
Sbjct: 617 LAAHPECILGEYPWLKYGSYETHKHLLSLVAEGQRDYSDCEHCGKPCAGNLAYECRRCKF 676

Query: 675 NVHAIGQCYHQQILKGKLSFKIK 698
           NVHAIG+CYH QI+K KLSFK+K
Sbjct: 677 NVHAIGRCYHDQIIKRKLSFKLK 681

BLAST of Lsi04G016790 vs. TAIR 10
Match: AT3G45530.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 248.8 bits (634), Expect = 1.3e-65
Identity = 200/678 (29.50%), Postives = 281/678 (41.45%), Query Frame = 0

Query: 26  HPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKFYIHQSCIHLPHKIRSRF-HPH 85
           HPH ++ ++    + + + C  C +S Y  A+ C  CKF +H+ C  L   +   F HP 
Sbjct: 8   HPHRVSLVK----NHDGLECDACDQS-YGDAYSCGECKFTVHKKCTFLFEVVPEIFEHPS 67

Query: 86  H----LLSLDTSNADH----CHCCWQMPCDCFYKCTHRGCEFIVDIKCTL------ADTK 145
           H    L  L T   DH    CH C +      Y C  R C+  VDI C +      AD  
Sbjct: 68  HVGHCLKLLTTGAPDHTVPKCHLCGKNTKRLLYHC--RDCKLNVDIDCIIDPMCAQADLN 127

Query: 146 RPGLNSIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAEL 205
            P          H HPL + N   T   + C +C    V G  Y C  C +     CA L
Sbjct: 128 MPW---------HHHPLFMGN-FATN--MTCDVCYDNHVDG--YICLCCRLFVGWVCAAL 187

Query: 206 ---PQEILQPNQHHHPLFLFPHAK---LHSFCNSCKNECSQFVYSCVHCNFNLHVMCRAS 265
                EI  P    HPL L            C+ C  +  +F+Y C  C FNL ++C  +
Sbjct: 188 VIDSPEITHPCHTKHPLKLLTEGAPTYTDPKCHLCGEDTGKFIYHCDICKFNLDLVCAKT 247

Query: 266 SK----------HKHNFTRLRTRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSL 325
                       H+H    +   I F C  CG  G   P+ C  C  + H+KCA LP  +
Sbjct: 248 KTPPVALSNLKVHEHTLNLMPRLISFVCDACGTKGDRAPYVCHQCDFMVHKKCAHLPRVI 307

Query: 326 LVVGHDCPLNFTYVHPFGVQTKLACDICRKKVEPQFAAYNCSKC-SYVVHLNCAGKKYLR 385
            V  HD  ++F Y  P G+  +  C +C ++++    AY+CS C  Y +H  CA +K + 
Sbjct: 308 NVNHHDHRVSFKY--PLGL-GEWRCGVCFEEIDWSCGAYSCSLCPQYAIHSLCATRKDVW 367

Query: 386 GLEQHDGRLYTGGRGRLKSSSQICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQE 445
             ++ DG                 I P    D  +                I H   E  
Sbjct: 368 NGKELDG----------VPEEVEDIEPFKRNDDNT----------------ITHFAHEHN 427

Query: 446 LILCKEEVDNDKQCHGCMQRFSVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLT 505
           L+   ++ +    C  C+     +   Y  S   C F LH+ C ++   KRH L   PL 
Sbjct: 428 LMSLSKDGEESSLCGACVLPIG-SYTFYKCSKSNCSFILHEKCANISKKKRHFLSSEPLI 487

Query: 506 LI--ATQDVAFQCHACLQ-FCHGFAYHCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQ 565
           L   +  D  F C AC Q FC GF Y  +      D+ C  I T    H SH H L   +
Sbjct: 488 LCFESIIDDDFLCRACYQMFCEGFFYSSKG--VNFDLICSSI-TMPFIHGSHDHHLLYVK 547

Query: 566 NHE--DQTCRGC-GQRNKTVFECDDGCNNFCLDYRCATLPQKVRC-KFDGSLLFLTFSVE 625
             E   +TC  C  +R K +  C   CN F LD+RCAT+P  +   ++D   L L +  E
Sbjct: 548 QEEGNKKTCHNCDNERAKVLLGCIK-CNYF-LDFRCATMPLTIMLPRYDDHPLTLCYGEE 607

Query: 626 DETGEYYCDVCEEERNPAGCFYYCKTCRLAAHTECILGEYPWLKYGSYETHKHLLALVTE 665
             +G+Y+CD+CE E NP   FY C  C +  HT C+LG+    K G    H   L L+  
Sbjct: 608 KASGKYWCDICERETNPETWFYTCNDCGVTLHTFCVLGDIRNAKAGGSFRHHGELELLPN 629

BLAST of Lsi04G016790 vs. TAIR 10
Match: AT1G65180.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 241.9 bits (616), Expect = 1.5e-63
Identity = 182/614 (29.64%), Postives = 262/614 (42.67%), Query Frame = 0

Query: 45  CCVCKKSLYPPAFICSLCKFYIHQSCIHLPHKIRSRFHPHHL----LSLDTSNAD---HC 104
           CC  +       + C  C+++ H+SC + P +I    HP H     LSL T+ A     C
Sbjct: 26  CCGLQFKTISDGYNCRKCRYFFHKSCSNCPKEIN---HPSHKCDKNLSLITNFASWSVRC 85

Query: 105 HCCWQ--MPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLNSIAKHFSHSHPLILENEIGT 164
             C +   P    Y+C  + CEF + + C     K P    I    +H H L LE     
Sbjct: 86  RTCKETIQPYIQNYEC--QRCEFNIHLDC----FKYPPPKVIDFPQNHDHKLKLEM---V 145

Query: 165 RRLVVCHLCGMLLVSGPAYFCSKCNIRFHKDCAELPQEILQPNQHHHPLFLF---PHAKL 224
           R    C  CG    SG  Y C +C++ FH DC +   E+  P+   H L L    P A  
Sbjct: 146 RSSFTCFTCGK-GGSGYPYKCHECDLAFHVDCEKYGAEVNHPSHSLHTLKLITGKPPAYT 205

Query: 225 HSFCNSC-KNECSQFVYSCVHCNFNLHVMC---------RASSKHKHNFTRLRTRIRFTC 284
              C+ C K    +  Y C  CNF L + C            + H H  T +   I FTC
Sbjct: 206 DGNCSLCGKKIFDEMFYHCSACNFTLDLRCVSLPPPLNLHDQNTHNHELTLMPKLISFTC 265

Query: 285 LLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQTKLACDIC 344
             CG  G   P+ C  C+  +H  C+  P  + +  HD  ++ T +   G+   + C +C
Sbjct: 266 TTCGLHGDRSPYVCLQCNFTSHNNCSGFPWVININRHDHRVSRTSL--LGIIDSV-CGVC 325

Query: 345 RKKVEPQFAAYNCSKCS-YVVHLNCAGKKYL-RGLEQHDGRLYTGGRGRLKSSSQICINP 404
           RKK++     Y+C +CS    H  CA ++ +  G+E  D                    P
Sbjct: 326 RKKMDWSCGGYSCKRCSESTFHTKCATREDVWDGIEMKD-------------------EP 385

Query: 405 LGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKE-EVDNDKQCHGCMQRFSVTKP 464
             DE         + FE +   I    S+ E  L L K      ++ C  C        P
Sbjct: 386 EEDE-------PMEPFEVISEGIIRHFSHIEHNLRLDKSGTFVEERSCKAC------AYP 445

Query: 465 SYS---YSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHACLQFCHGFAY 524
            Y    YSC+ C F LH++C   P  KRH L   P  L A+Q    +C AC  F + F Y
Sbjct: 446 IYHDPFYSCMSCDFLLHENCAKHPTRKRHVLSNKPYILHASQRNYSKCKACGVFFNCFIY 505

Query: 525 HCEECLYTLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGCNN 584
                 + LD+RC    +  L HPSH H   L        C  C +    V  C +    
Sbjct: 506 GTFP--FGLDVRCASF-SGSLVHPSHQH--PLFYTSPKGVCSACNKETSHVLRCVEDKCG 565

Query: 585 FCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCRLA 631
           + LD++CA LP +V+ + D   L L +   D +G+Y+CD+CE+E +P   FY CK C + 
Sbjct: 566 YALDFKCALLPHEVKHRVDDHFLSLCYGERDASGKYWCDICEKETDPTKWFYTCKDCGVT 586

BLAST of Lsi04G016790 vs. TAIR 10
Match: AT3G27500.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 241.9 bits (616), Expect = 1.5e-63
Identity = 212/714 (29.69%), Postives = 295/714 (41.32%), Query Frame = 0

Query: 5   ELEELEAEGSEMEKLKLLEQPHPHPLTYIEERTDSREDMFCCVCKKSLYPPAFICSLCKF 64
           ELEELE               H H    +  R    + + C  C + L    + CS CKF
Sbjct: 4   ELEELEG--------------HEH----LVSRVKHHDGLECDACDR-LNVDGYSCSECKF 63

Query: 65  YIHQSCIHLPHKIRSRFHP----HHLLSLDTSNADH----CHCCWQMPCDCFYKCTHRGC 124
            IH +C  +  K     HP    H L  L T   DH    CH C        + C+   C
Sbjct: 64  NIHVACTFVFDKQEIVDHPSHDGHSLKLLTTGAPDHTDPKCHLCGNNTKRLLFHCS--VC 123

Query: 125 EFIVDIKCTLADTKRPGLNSIAKHFSHSHPLILENEIGTRRLVVCHLCGMLLVSGPAYFC 184
           +  +DI C       P   ++     H HPL+   + G+R    C  C      G  Y C
Sbjct: 124 QLNLDIFCIADHLLGPEYLNMP---WHPHPLLC-FDFGSRNW--CDFCNE-NYEGQGYLC 183

Query: 185 SKCNIRFHKDCAELPQEILQ-PNQHHHPLFLFPHAK---LHSFCNSCKNECSQFVYSCVH 244
            +C +R H+ C    QEI Q P+   HPL L         +  C+ C  +    +Y C  
Sbjct: 184 YRCGLRIHEKCVVELQEITQHPSHVRHPLKLLTSGAPNYTNPRCHICGEDTGNLLYHCDI 243

Query: 245 CNFNLHVMCRASSK----------HKHNFTRLRTRIRFTCLLCGWPGLDFPWFCSICHLL 304
           C FNL + C   S           H+H  T +   I F C  CG  G   P+ C  C  +
Sbjct: 244 CKFNLDLQCAIKSSPPVALSNMKVHEHTLTLMPKLISFVCDACGTKGDRSPYICVQCDFM 303

Query: 305 A-HEKCAELPPSLLVVGHDCPLNFTYVHPFGVQTKLACDICRKKVEPQFAAYNCSKCS-Y 364
             H+KCA LP  + V  HD  ++F Y  P G      C +C  +++  + AY+CS CS Y
Sbjct: 304 TFHQKCARLPRVIHVNHHDHRVSFKY--PLG-PGDWRCGVCWDEIDWSYGAYSCSLCSRY 363

Query: 365 VVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQICINPL-GDEDATSNNFSKKTFEEV 424
            +H  CA +      +  DG    G    ++      I P   ++D T ++FS       
Sbjct: 364 AIHSLCATRD-----DVWDGEELDGVPEEIED-----IEPFKRNDDNTIDHFS------- 423

Query: 425 GNLIEILHSNCEQELILCKEEVDNDKQCHGCMQRFSVTKPSYSYSCVGCGFFLHKDCVDL 484
                  H +    L    EE    K C        +   S  Y C  C F LH+ C +L
Sbjct: 424 -------HRHNHMSLDKGGEESSFCKAC-----AHPIGSSSSLYKCSECSFILHETCANL 483

Query: 485 PITKRHPLHKHPLTL----IATQDVAFQCHACLQ-FCHGFAY--HCEECLYTLDIRCVLI 544
           P+ KRH L   PLTL    +  +     C AC Q FC GF Y  + EE     D+ C  I
Sbjct: 484 PMKKRHFLSPQPLTLSYHRLQRRWGVEICEACQQIFCKGFIYSSYLEE---NFDLPCSSI 543

Query: 545 KTKKLKHPSHGH--LLSLAQNHEDQTCRGCGQRNKTVFECDDGCNNFCLDYRCATLPQKV 604
            T    H SH H  L    +N   +TC+GCG   K V      CN F LD+RCATLP  V
Sbjct: 544 -TVPFIHGSHDHHLLYLKLENGNVKTCQGCGIDEKRVAIGCTKCNYF-LDFRCATLPLTV 603

Query: 605 R-CKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCRLAAHTECILGEYPWL 664
              ++D   L L +  E  +G+Y+CD+CE E NP   FY C  C +  H  C++G+  + 
Sbjct: 604 TLSRYDDHPLTLCYGDEKASGKYWCDICERETNPKTWFYTCNDCGVTLHVLCVVGDVRYA 649

Query: 665 KYGSYETHKHLLALVTEGKMNYSDCDHCGKPCAGNLAYECCRCKFNVHAIGQCY 684
           K       ++ + L++    +   C +C   C G       R + + H  G+ Y
Sbjct: 664 K--PKAMIEYYIELLSNNSSSRPFCHNCYCRCPGPFILR-IRKQHDAHLGGEYY 649

BLAST of Lsi04G016790 vs. TAIR 10
Match: AT5G22355.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 241.5 bits (615), Expect = 2.0e-63
Identity = 189/648 (29.17%), Postives = 277/648 (42.75%), Query Frame = 0

Query: 60  SLCKFYIHQSCIHLPHKIRSR-FHPHHLLSLDTSNADHCHC--CWQMPCDCFYKCTHRGC 119
           S    + H+ C   P +I +   HP H L L  S  + C C  C +      Y+C+ + C
Sbjct: 45  SSTSLFFHKECAEAPPEIINHPSHPQHTLKLHNSLRNPCKCNLCGKTFFAFGYRCSSK-C 104

Query: 120 EFIVDIKCTLADTKRPGLNSIAKHFSHSHPLILENE---IGTRRLVVCHLCGMLLVSGPA 179
           +FIVD+ C +     P   SI    SH HP+I   E    G RR  +C         G +
Sbjct: 105 DFIVDLTCGI----NPLPVSIEHPKSHHHPVIFLKEPAKPGRRRCGICKGYN----GGCS 164

Query: 180 YFCSKCNIRFHKDCAELPQEILQPNQHHHPLFLFPHAKLHS----FCNSCKNECSQFVYS 239
           Y C +C + FH +C  L QE+  P+   H L L  +  L S     C  C     + +Y 
Sbjct: 165 YACLECEVHFHVECVNLSQEVNHPSHPQHSLKLLEYESLTSDAEETCLLCGERPDKVLYR 224

Query: 240 CVHCNFNLHVMCRAS---------SKHKHNFTRLRTRIRFTCLLCGWPGLDFPWFCSICH 299
           C  CNF++   C              H+H    L   I F C  CG  G   P+ C  C 
Sbjct: 225 CSICNFSVCRFCTKDPPPLAIEHHKTHEHRLVLLSRLISFECNACGMQGDRSPYMCVQCG 284

Query: 300 LLAHEKCAELPPSLLVVGHDCPLNFTYVHPFGVQTKLACDICRKKVEPQFAAYNCSKC-S 359
            + H  C +LP  + +  HD  ++FT  H  GV     C ICRK +     AY C +C +
Sbjct: 285 FVVHRTCIDLPRVININRHDHRISFT--HHLGVGYS-RCGICRKDISQYHGAYLCPRCPN 344

Query: 360 YVVHLNCAGKKYLRGLEQHDGRLYTGGRGRLKSSSQICINPLGDEDATSNNFSKKTFEEV 419
           Y  H  CA +K     +  DG    G                 DED          F+ V
Sbjct: 345 YAAHSLCATRK-----DVWDGVELEGTPDD------------DDEDIV-------PFKVV 404

Query: 420 GNLIEILHSNCEQELILCKEEVDNDK--QCHGCMQRFSVTKPSYS---YSCVGCGFFLHK 479
           G+ +    S+ E  L L K+ ++ D+  +C  C+       P YS   Y+C  C F LH+
Sbjct: 405 GDNLIKHFSHEEHNLRLNKDNINRDEGSRCEACV------LPIYSDPIYNCEECRFILHE 464

Query: 480 DCVDLPITKRHPLHKHPLTL-------IATQDV----AFQCHACLQFCHGFAYHCEECLY 539
            C + P  KRH  H  P TL         ++D      F+C+AC     GF Y  +   +
Sbjct: 465 KCANHPKKKRHVFHTKPFTLWSRPPRTFHSKDFRFYDVFRCYACRTKSTGFRYVSD--WW 524

Query: 540 TLDIRCVLIKTKKLKHPSHGHLLSLAQNHEDQTCRGCGQRNKTVFECDDGCNNFCLDYRC 599
            LD+RC   +++ + H  H H L   ++ +D  C  C +         D C +F LD  C
Sbjct: 525 VLDVRCG-SRSEPVIHDGHRHPL-YYEHKKDHCCDACYKEIDGYLLSCDTC-DFDLDLHC 584

Query: 600 ATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPAGCFYYCKTCRLAAHTECIL 659
             LP+ V+   D   L L +  E+ TG+Y+CD+CE E +P+  FY C  C + AH EC+L
Sbjct: 585 TDLPKTVKHSCDNHPLSLCYG-ENATGKYWCDICEAETDPSKWFYTCSKCVVTAHIECVL 644

Query: 660 GEYPWLKYGSYETHKHL-LALVTEGKMNYSDCDHCGKPCAGNLAYECC 671
           G++  L  G    + ++ + +V     +   C  C   C   +  + C
Sbjct: 645 GDFSRLMPGRIINYNNVRVEVVLNSYSSRPFCAKCHSRCRAPIILKLC 644

BLAST of Lsi04G016790 vs. TAIR 10
Match: AT4G01760.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 240.0 bits (611), Expect = 5.9e-63
Identity = 190/658 (28.88%), Postives = 281/658 (42.71%), Query Frame = 0

Query: 45  CCVCKKSLYPPAFICSLCKFYIHQSC-----------IHLPHKIR----SRFHPHHLLSL 104
           CC   +++    + C+ C F++H+ C            H  H +R    SR    H L +
Sbjct: 27  CCERLEAI-SDGYYCTTCDFFVHKKCGDEASECIENPFHSNHPLRLGFLSRLQRQHWLKV 86

Query: 105 DTSNADHCHCCWQMPCDCFYKCTHRGCEFIVDIKCTLADTKRPGLNSIAKHFSHSHPLIL 164
             S    C  C +   D FY+C    C+F +D+ C     K P L  I     HSH L  
Sbjct: 87  VRS----CDLCGKNIGDLFYRC--EICDFDMDLHC----AKYPPLEVIDIPEMHSHKL-- 146

Query: 165 ENEIGTRRLVVCHL-CGMLLVSGPAYFCSKCNIRFHKDCAEL--------PQEILQPNQH 224
            N +  R    C   CG +    P Y C +C+ +FH DC           P E+      
Sbjct: 147 -NLLKDRVEFDCDAKCGKIGYGFP-YECHECDSKFHVDCVRYSSSEEVKHPLEVNHSYHS 206

Query: 225 HHPLFLF---PHAKLHSFCNSCKNEC-SQFVYSCVHCNFNLHVMCRAS---------SKH 284
            HPL L    P       C  C  +   +  Y C  CNF L + C  +           H
Sbjct: 207 LHPLKLLMGQPPDYSDGKCRLCGRKIDDKLFYHCSSCNFTLDMRCVLNPPPKSVLDLKTH 266

Query: 285 KHNFTRLRTRIRFTCLLCGWPGLDFPWFCSICHLLAHEKCAELPPSLLVVGHDCPLNFTY 344
            H    L   I FTC  CG  G   P+ C  C  L H+ C  LP  + +  HD  ++ T 
Sbjct: 267 DHQLNLLPRLIFFTCNACGLNGDRSPYACFQCDFLIHKDCFGLPRLININRHDHRISRTS 326

Query: 345 VHPFGVQTKLACDICRKKVEPQFAAYNCSKCS-YVVHLNCAGKKYLRGLEQHDGRLYTGG 404
           +   G+   + C +CR+KV+     Y+C +CS Y+ H  CA ++     +  +G+   G 
Sbjct: 327 L--IGIVNSV-CGVCRQKVDWTCGGYSCQRCSTYIAHSKCATRE-----DVWNGKELEGV 386

Query: 405 RGRLKSSSQICINPLGDEDATSNNFSKKTFEEVGNLIEILHSNCEQELILCKEEVDNDKQ 464
              ++      +     +D T  +FS K      N+  ++   CE            +K+
Sbjct: 387 PEEIEDIEPYVV----IDDNTIQHFSHKEHYMRLNVNGLM---CEV-----------NKR 446

Query: 465 CHGCMQRFSVTKPSYSYSCVGCGFFLHKDCVDLPITKRHPLHKHPLTLIATQDVAFQCHA 524
           C+ C    S   P   Y C+ C F LH++C   P  K H LH   L L+ ++   F C A
Sbjct: 447 CNACNHPIS---PQSFYGCMDCDFILHQNCAGFPRRKWHVLHNERLALVTSEVNIFGCSA 506

Query: 525 CLQFCHGFAYHCEECLYTLDIRCVLIKTKKLKHPSH-GHLLSLAQNHEDQTCRGCGQRNK 584
           C +  +GF Y  E+    LD+ C    ++   HPSH  H L      +D+ C GC +R+ 
Sbjct: 507 CHKIFNGFRYEHEDT--KLDVLCGSF-SEPFIHPSHPHHPLYCISPEDDEVCSGCNERSY 566

Query: 585 TVFEC-DDGCNNFCLDYRCATLPQKVRCKFDGSLLFLTFSVEDETGEYYCDVCEEERNPA 644
            V  C +D C  F LD+ CAT PQ V+ + +   L L +  E  +G+Y+CD+CE+E NP 
Sbjct: 567 HVLRCIEDNC-GFILDFGCATFPQVVKHRIEDQPLSLCYG-EKASGKYWCDICEKETNPN 626

Query: 645 GCFYYCKTCRLAAHTECILGEYPWL-KYGSYETHKHLLALVTEGKMNYSDCDHCGKPC 662
             FY CK  R + HT C+LG++  L    + E       +V    ++   C HC   C
Sbjct: 627 TWFYTCKDHRASLHTWCVLGDFMGLIPKSTIELWNISYEVVLNNSISRPICRHCKSHC 635

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LDB00.0e+0079.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G857600 PE=4 SV=1[more]
A0A6J1F8C30.0e+0078.92uncharacterized protein LOC111443068 OS=Cucurbita moschata OX=3662 GN=LOC1114430... [more]
A0A5D3D8660.0e+0079.65DC1 domain-containing family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CRK20.0e+0079.65uncharacterized protein LOC103503938 OS=Cucumis melo OX=3656 GN=LOC103503938 PE=... [more]
A0A6J1IGZ88.9e-27778.48uncharacterized protein LOC111475038 OS=Cucurbita maxima OX=3661 GN=LOC111475038... [more]
Match NameE-valueIdentityDescription
XP_038898645.10.0e+0082.99uncharacterized protein LOC120086189 [Benincasa hispida][more]
XP_023536436.10.0e+0078.16uncharacterized protein LOC111797614 [Cucurbita pepo subsp. pepo] >XP_023536438.... [more]
XP_004147882.10.0e+0079.36uncharacterized protein LOC101207273 [Cucumis sativus] >KGN59970.1 hypothetical ... [more]
XP_022936454.10.0e+0078.92uncharacterized protein LOC111443068 [Cucurbita moschata][more]
KAG7024193.10.0e+0078.92hypothetical protein SDJN02_13007, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G45530.11.3e-6529.50Cysteine/Histidine-rich C1 domain family protein [more]
AT1G65180.11.5e-6329.64Cysteine/Histidine-rich C1 domain family protein [more]
AT3G27500.11.5e-6329.69Cysteine/Histidine-rich C1 domain family protein [more]
AT5G22355.12.0e-6329.17Cysteine/Histidine-rich C1 domain family protein [more]
AT4G01760.15.9e-6328.88Cysteine/Histidine-rich C1 domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 426..488
e-value: 9.1
score: 6.5
coord: 160..220
e-value: 0.97
score: 17.8
coord: 318..381
e-value: 4.9
score: 9.7
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainSMARTSM00109c1_12coord: 26..76
e-value: 0.12
score: 14.5
coord: 143..193
e-value: 0.02
score: 21.8
coord: 309..352
e-value: 0.51
score: 8.6
coord: 247..293
e-value: 0.1
score: 15.1
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 144..195
score: 7.9711
IPR004146DC1PFAMPF03107C1_2coord: 299..347
e-value: 2.2E-7
score: 31.1
coord: 26..71
e-value: 4.9E-7
score: 30.0
coord: 466..510
e-value: 3.1E-8
score: 33.8
coord: 141..188
e-value: 4.0E-8
score: 33.5
NoneNo IPR availablePANTHERPTHR32410:SF178CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEINcoord: 24..149
NoneNo IPR availablePANTHERPTHR32410CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEINcoord: 24..149
NoneNo IPR availablePANTHERPTHR32410CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEINcoord: 141..682
NoneNo IPR availablePANTHERPTHR32410:SF178CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEINcoord: 141..682
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 170..242
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 547..630
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 274..349
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 48..127
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 440..513

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G016790.1Lsi04G016790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
molecular_function GO:0046872 metal ion binding