Lsi04G016420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G016420
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionJ domain-containing protein required for chloroplast accumulation response 1
Locationchr04: 23897160 .. 23901273 (-)
RNA-Seq ExpressionLsi04G016420
SyntenyLsi04G016420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATATGTCGCAGAGAGAGAGCATTCTTTTAGGTTATTCGCTTCAGAGATCATTCACTAACCCTTCTTCATCGCCGAGAGCTTCCAATCGGAACTCCGATGATGTTGATTTTCACGACGTGTTTGGTGGTCCTCCGAGGAGGCGGTCATCGGTTCATGAAACGCGGTATAGCTTCTCTGAGACGGGGGATTCCTTCTCATTGAAAGGCGGCGATGATGAAGCGTTACCCGGCCGGAGTGGCCCCTGGTCCGGTTTGAATGAGAAACCGGTGTTTGGAGAAGAAGGTGTACACGGGCGGCGATTTCCGAGCGATGATTTTTACGATGATATCTTTAAAGGCGATGAATCGGTGAATTCTTCTCCTCGCCGGCATGAGCGTGACATTTTCTCTTCGATTCCTGGTTCGAAGGTTCTAAGTCCTGCTAGACCTCTTCCGCCGCCGGCGGAACCCTTCGGAAGTTCTTCCCTCCCTGCACAATTAAGGTTCTTCTTCGCCCGTCTTCCATTTCTTGATTTGATAATGGGTTTCATTCCAAATTCTTATTGGTGGCTAATTTTCTAACCGTTGATGTATGGCTCTTTGTTTCTGGTTCTGATGTTCTTCTTGTGGTTATGGCTGCGTTGTGTTTGTTTTTTTGTTTCTCTGCATTGGGTATTTGTTAATATCTTGCTTCTGCTTCTTTTGGTTTGTGGCAGCCTACCATCAAGATTAGCCAAAGGGACTGATTTACCGGCGTTTGGATCGAGCTCGTTAAGAAACAAGGACAGTGTTTCGAATGGAAGTCATACAAATTCTTCTAGGTTAACTCTTTCTAGATTTTCTTCTAGCACATCAAGCCACCGTTTCGAGGATCTTAAAACTGATTACAATTTGCCTGATCGTACCGGTCTCTTGTCATCTGAATTCCAAGAACATGGCAATGAGGAAGCATCGTCCTTCAGAAAGTCTGATAATGCATTGAGTGTGAGCAGCTTAACGAAGGGAGTAGAAGATAGTTTAGAAGAATCAAATGGTGGTGGTCAATTTCAGTTCCATTTCTCAATTTACAAATGGGCAAGCAAAGGGGTGCCTCTGATGATGCCATTGAGAGGAGGGAACGGATCGAGATTAAGAGAAAAGACTTTGCTTAGAAGAAGCTCGAGCTCGACCGATAGGGTAATGAAGGCGAAAAATGAAATCCATTCCCCAACATCGACTACACAGAATATTGATTTTCCTCCTATTTTTCATGAGAGGATGAACGTCGATGATGAAAAAGGAACTGATTTACTGCCTGACATAGAGACTGTTGATCAAAGACAAAGTTCATCTGCACTTTCAGAAAACTTGAGCAGACAAAGTTCTCGTAAGGATGTTGACAGTGATAACATCGGTCGCCCAAGGGAAAAAGAGAAACCTCATTCTTTTCCCAAGAAAGTTTCAAGTGAAAAATCAGAGAAAAACGTGGCTTCGACAACGAACGAAGATCAAAAGCATGAAGCTAAATCTCTAAGTTCCTTTCTTCTCTACAGTGATAGTGAACAAAGTATGTTCTGCAAAGACTAGGAGACAATTTTTCTTTTCTTGATCATATTATGTGGTTCTCACAGCTAGTTTCCTTTCATGCAGGTGAAGAAGGGATAGGTAAAGAATATCGTAAAGGGGAAATCATGGCAAAAAGTGACAAGAAATCATCAATTCTTTCTGATCTAAGTAGTAGCCCAAAGAAACAGGACAAACAAACTCTAAAAGTGAAAAAACCTAGTCCCCTAAGTTCAGACATAGAATCTGGGCATAACATTGGCAGAAAGAAAGTTGGTGGAAAAATTTCAGAGTTTGTTAAGCTTTTCAACCAAGAACCTACATCGAAACCCCGAGATGTAGTCGATTCAGAAAATGATAGCTCTACAATGAAACAGGAAAGTACTTCAAAAGCTCAAACAGAAGCAACCGTCAATAAAATAAGGAAGGATGAGAAACCCAAGTTGAATAGGAATACAGATTCTTCCATCAATGTGAGTAACTATGAGAAGTTAACCAGCCACTCTTTTTTACACCATTGAAGATAAGAATTTGCAAGTCAAAAATAATTTTGATGGACCTTAATTTTCAATCTTTATAGGGAGATGATGTTTCCAAGCATTCAATGGATGATCACTCTGCTAAAAGTGCTGCTAGCTACAGAAGTAGCTTTGCTTCTTCCAAAGAGGGTAGTCCAGCTCCAAACACTGGTTAGTCATGCTTCTGTGCTGCGCACTTGATATTTTTTTTCTGTCCAAAAGGAAGTAGACGTACTCGTGATTTCTTTCAATCATCCATTGCATAACATATTACTTCTCGATGTAGTTCACGTTCCCGATGTCGCAAAGTCTACAGTTCCAGATGTGGAGGATCCCTTCCAGGAGAATTTTTCGGTATGTTTATCCTTGTGGAAATGAAATTTCGGTTCAATCTCTTTCAATCGATTCTATCTACTGTTCCGCCCACAAATTGCTTAGATCCCAAAGTGTTCATTTACCTATCTCTTAGGGTGTGTTTGGTTTAACTTTTCAAGTGTTTGATTTTGAAAATAAGTCATTTTGGAAAAAGTTGAAGTGTTTGGCAACCTCTGAAAACAACTTTTGAAACACTTTCAAAGTGTAGTTTAAACCGTTTTTATCAAAAGAGTTTAAATAAAAATGAATATTTTGAAAAACACTTTTTTCTTTAGTCAATCCAAACAGACCCTTAATCCTTGGAGAGTACAAAATTATGCATCGAATATATTCGCGTATGAAACGTGAAACGTAGTCAAATGCTTTGTATCAATCAATTTCCAACAATCTAACGAGCTGATTTGGTTGGTTTCTGAACAGGTAAAAGAGTTACCAGAAGACTATGACAAATCCACAGAAACAAACAATGGCCGTGAAGAACTACAAGTAATGCATCTCACATTAAATAAAAGTATTGTGTTTATTGCAGCACGTGACGAAGCAAGCTTATAAGAACTTCTTGCGATGCAGGCTATCGATACTAAAATACGACAATGGTCAAACGGGAAGGAAGGGAATATACGTTCGCTGCTATCAACTTTGCAATATGTGAGTCTGTTATCTTATTAACTCCGTTGGTTGCCAAGCAATTTGCATACTCTGGGATTGCCTTGCTTTACCAATAAGAACCTGTCCTGTCAATCTGATTGTTACATTTGTAGTTCGTTCTTGAGGTCTACGCTCGACGTTCTTCAATTTCTATAAACTGTTCTGGTTGTATGCAAGGTTCTTTGGCCCAAGAGCGGATGGAAACCCGTTCCTCTCGTTGATATAATCGAAGGAAATGCAGTCAAAAGATCTTATCAGAAAGCTTTGTTATACCTACATCCTGATAAGCTACAACAGAAGGGTGCTTCATCAGATCAAAAATATATTGCAGCAAAAGTTTTTGAAATATTACAGGTATTCAAGTTTACTTCCATATTCAGAATTTACAACCAAACATGATTTCTGTCTTTCAAGGGTGTTTTAGTCCAATGTCATGAATCATGATAAAACAGTTATACTTCTACAAACTTTGAGACCAGTCTATCTTGTAAATGCTTCTATAAGTAGCTTGTGATTTGTTATGAGAAGCTGAAAATTTGAACGTTTTAAAGAGTCAGGATTTCTGATGTTTCTTTTTTTAGTACTATAACAAATGGAGGTGGAAATTCGAACATTTAACCTTAACCATTTGAGTTATAGACAGAGCAAAGTTTCCAATTCTTAACGATTGGAGTAGTCTGTGTTATTTTCATTTGTGTTAATGAAAGTTTAACAGTTGGATGTTAAATCTTTGCATTGGTGTAGGAGGCTTGGACTCATTTCAACACGCTTGGTGGATTATGACATAAAAACATAGGAGAAACTATGATTTCGGAAAAATGGAAGGAAATGAGGTGCACACAGAAGAGGCGTCTGTATAAAGCTTTTTAAAGTGTCATTCGAAATGTCCTTTAATAAAGAAAAATAATTGAAATATTTTTCTTCTTTTATCATTTATAGAATAATACTTTTCATTATTTGATTATCACAATCATAAAAAATTACTATGATTAAATCTTTGTCATTCATGAGCATTAAAATTTTTCTAGTCATTCACTTTATTTTAA

mRNA sequence

ATGGAGAATATGTCGCAGAGAGAGAGCATTCTTTTAGGTTATTCGCTTCAGAGATCATTCACTAACCCTTCTTCATCGCCGAGAGCTTCCAATCGGAACTCCGATGATGTTGATTTTCACGACGTGTTTGGTGGTCCTCCGAGGAGGCGGTCATCGGTTCATGAAACGCGGTATAGCTTCTCTGAGACGGGGGATTCCTTCTCATTGAAAGGCGGCGATGATGAAGCGTTACCCGGCCGGAGTGGCCCCTGGTCCGGTTTGAATGAGAAACCGGTGTTTGGAGAAGAAGGTGTACACGGGCGGCGATTTCCGAGCGATGATTTTTACGATGATATCTTTAAAGGCGATGAATCGGTGAATTCTTCTCCTCGCCGGCATGAGCGTGACATTTTCTCTTCGATTCCTGGTTCGAAGGTTCTAAGTCCTGCTAGACCTCTTCCGCCGCCGGCGGAACCCTTCGGAAGTTCTTCCCTCCCTGCACAATTAAGCCTACCATCAAGATTAGCCAAAGGGACTGATTTACCGGCGTTTGGATCGAGCTCGTTAAGAAACAAGGACAGTGTTTCGAATGGAAGTCATACAAATTCTTCTAGGTTAACTCTTTCTAGATTTTCTTCTAGCACATCAAGCCACCGTTTCGAGGATCTTAAAACTGATTACAATTTGCCTGATCGTACCGGTCTCTTGTCATCTGAATTCCAAGAACATGGCAATGAGGAAGCATCGTCCTTCAGAAAGTCTGATAATGCATTGAGTGTGAGCAGCTTAACGAAGGGAGTAGAAGATAGTTTAGAAGAATCAAATGGTGGTGGTCAATTTCAGTTCCATTTCTCAATTTACAAATGGGCAAGCAAAGGGGTGCCTCTGATGATGCCATTGAGAGGAGGGAACGGATCGAGATTAAGAGAAAAGACTTTGCTTAGAAGAAGCTCGAGCTCGACCGATAGGGTAATGAAGGCGAAAAATGAAATCCATTCCCCAACATCGACTACACAGAATATTGATTTTCCTCCTATTTTTCATGAGAGGATGAACGTCGATGATGAAAAAGGAACTGATTTACTGCCTGACATAGAGACTGTTGATCAAAGACAAAGTTCATCTGCACTTTCAGAAAACTTGAGCAGACAAAGTTCTCGTAAGGATGTTGACAGTGATAACATCGGTCGCCCAAGGGAAAAAGAGAAACCTCATTCTTTTCCCAAGAAAGTTTCAAGTGAAAAATCAGAGAAAAACGTGGCTTCGACAACGAACGAAGATCAAAAGCATGAAGCTAAATCTCTAAGTTCCTTTCTTCTCTACAGTGATAGTGAACAAAGTGAAGAAGGGATAGGTAAAGAATATCGTAAAGGGGAAATCATGGCAAAAAGTGACAAGAAATCATCAATTCTTTCTGATCTAAGTAGTAGCCCAAAGAAACAGGACAAACAAACTCTAAAAGTGAAAAAACCTAGTCCCCTAAGTTCAGACATAGAATCTGGGCATAACATTGGCAGAAAGAAAGTTGGTGGAAAAATTTCAGAGTTTGTTAAGCTTTTCAACCAAGAACCTACATCGAAACCCCGAGATGTAGTCGATTCAGAAAATGATAGCTCTACAATGAAACAGGAAAGTACTTCAAAAGCTCAAACAGAAGCAACCGTCAATAAAATAAGGAAGGATGAGAAACCCAAGTTGAATAGGAATACAGATTCTTCCATCAATGGAGATGATGTTTCCAAGCATTCAATGGATGATCACTCTGCTAAAAGTGCTGCTAGCTACAGAAGTAGCTTTGCTTCTTCCAAAGAGGGTAGTCCAGCTCCAAACACTGTTCACGTTCCCGATGTCGCAAAGTCTACAGTTCCAGATGTGGAGGATCCCTTCCAGGAGAATTTTTCGGTAAAAGAGTTACCAGAAGACTATGACAAATCCACAGAAACAAACAATGGCCGTGAAGAACTACAAGCTATCGATACTAAAATACGACAATGGTCAAACGGGAAGGAAGGGAATATACGTTCGCTGCTATCAACTTTGCAATATGTTCTTTGGCCCAAGAGCGGATGGAAACCCGTTCCTCTCGTTGATATAATCGAAGGAAATGCAGTCAAAAGATCTTATCAGAAAGCTTTGTTATACCTACATCCTGATAAGCTACAACAGAAGGGTGCTTCATCAGATCAAAAATATATTGCAGCAAAAGTTTTTGAAATATTACAGGAGGCTTGGACTCATTTCAACACGCTTGGTGGATTATGACATAAAAACATAGGAGAAACTATGATTTCGGAAAAATGGAAGGAAATGAGGTGCACACAGAAGAGGCGTCTGTATAAAGCTTTTTAAAGTGTCATTCGAAATGTCCTTTAATAAAGAAAAATAATTGAAATATTTTTCTTCTTTTATCATTTATAGAATAATACTTTTCATTATTTGATTATCACAATCATAAAAAATTACTATGATTAAATCTTTGTCATTCATGAGCATTAAAATTTTTCTAGTCATTCACTTTATTTTAA

Coding sequence (CDS)

ATGGAGAATATGTCGCAGAGAGAGAGCATTCTTTTAGGTTATTCGCTTCAGAGATCATTCACTAACCCTTCTTCATCGCCGAGAGCTTCCAATCGGAACTCCGATGATGTTGATTTTCACGACGTGTTTGGTGGTCCTCCGAGGAGGCGGTCATCGGTTCATGAAACGCGGTATAGCTTCTCTGAGACGGGGGATTCCTTCTCATTGAAAGGCGGCGATGATGAAGCGTTACCCGGCCGGAGTGGCCCCTGGTCCGGTTTGAATGAGAAACCGGTGTTTGGAGAAGAAGGTGTACACGGGCGGCGATTTCCGAGCGATGATTTTTACGATGATATCTTTAAAGGCGATGAATCGGTGAATTCTTCTCCTCGCCGGCATGAGCGTGACATTTTCTCTTCGATTCCTGGTTCGAAGGTTCTAAGTCCTGCTAGACCTCTTCCGCCGCCGGCGGAACCCTTCGGAAGTTCTTCCCTCCCTGCACAATTAAGCCTACCATCAAGATTAGCCAAAGGGACTGATTTACCGGCGTTTGGATCGAGCTCGTTAAGAAACAAGGACAGTGTTTCGAATGGAAGTCATACAAATTCTTCTAGGTTAACTCTTTCTAGATTTTCTTCTAGCACATCAAGCCACCGTTTCGAGGATCTTAAAACTGATTACAATTTGCCTGATCGTACCGGTCTCTTGTCATCTGAATTCCAAGAACATGGCAATGAGGAAGCATCGTCCTTCAGAAAGTCTGATAATGCATTGAGTGTGAGCAGCTTAACGAAGGGAGTAGAAGATAGTTTAGAAGAATCAAATGGTGGTGGTCAATTTCAGTTCCATTTCTCAATTTACAAATGGGCAAGCAAAGGGGTGCCTCTGATGATGCCATTGAGAGGAGGGAACGGATCGAGATTAAGAGAAAAGACTTTGCTTAGAAGAAGCTCGAGCTCGACCGATAGGGTAATGAAGGCGAAAAATGAAATCCATTCCCCAACATCGACTACACAGAATATTGATTTTCCTCCTATTTTTCATGAGAGGATGAACGTCGATGATGAAAAAGGAACTGATTTACTGCCTGACATAGAGACTGTTGATCAAAGACAAAGTTCATCTGCACTTTCAGAAAACTTGAGCAGACAAAGTTCTCGTAAGGATGTTGACAGTGATAACATCGGTCGCCCAAGGGAAAAAGAGAAACCTCATTCTTTTCCCAAGAAAGTTTCAAGTGAAAAATCAGAGAAAAACGTGGCTTCGACAACGAACGAAGATCAAAAGCATGAAGCTAAATCTCTAAGTTCCTTTCTTCTCTACAGTGATAGTGAACAAAGTGAAGAAGGGATAGGTAAAGAATATCGTAAAGGGGAAATCATGGCAAAAAGTGACAAGAAATCATCAATTCTTTCTGATCTAAGTAGTAGCCCAAAGAAACAGGACAAACAAACTCTAAAAGTGAAAAAACCTAGTCCCCTAAGTTCAGACATAGAATCTGGGCATAACATTGGCAGAAAGAAAGTTGGTGGAAAAATTTCAGAGTTTGTTAAGCTTTTCAACCAAGAACCTACATCGAAACCCCGAGATGTAGTCGATTCAGAAAATGATAGCTCTACAATGAAACAGGAAAGTACTTCAAAAGCTCAAACAGAAGCAACCGTCAATAAAATAAGGAAGGATGAGAAACCCAAGTTGAATAGGAATACAGATTCTTCCATCAATGGAGATGATGTTTCCAAGCATTCAATGGATGATCACTCTGCTAAAAGTGCTGCTAGCTACAGAAGTAGCTTTGCTTCTTCCAAAGAGGGTAGTCCAGCTCCAAACACTGTTCACGTTCCCGATGTCGCAAAGTCTACAGTTCCAGATGTGGAGGATCCCTTCCAGGAGAATTTTTCGGTAAAAGAGTTACCAGAAGACTATGACAAATCCACAGAAACAAACAATGGCCGTGAAGAACTACAAGCTATCGATACTAAAATACGACAATGGTCAAACGGGAAGGAAGGGAATATACGTTCGCTGCTATCAACTTTGCAATATGTTCTTTGGCCCAAGAGCGGATGGAAACCCGTTCCTCTCGTTGATATAATCGAAGGAAATGCAGTCAAAAGATCTTATCAGAAAGCTTTGTTATACCTACATCCTGATAAGCTACAACAGAAGGGTGCTTCATCAGATCAAAAATATATTGCAGCAAAAGTTTTTGAAATATTACAGGAGGCTTGGACTCATTTCAACACGCTTGGTGGATTATGA

Protein sequence

MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSFSETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVNSSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSSSLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEEASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSRLREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIETVDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVASTTNEDQKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQTLKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMKQESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASSKEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLGGL
Homology
BLAST of Lsi04G016420 vs. ExPASy Swiss-Prot
Match: Q9C9Q4 (J domain-containing protein required for chloroplast accumulation response 1 OS=Arabidopsis thaliana OX=3702 GN=JAC1 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.8e-73
Identity = 253/761 (33.25%), Postives = 372/761 (48.88%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSP--RASNRNSDDVDFHDVFGGPPRRRSSV---HE 60
           M+ +   E++LLG        + S+ P  R+   +  D+DF DVFGGPP+RRS V     
Sbjct: 1   MQTLPSSETVLLG--------SNSAPPVLRSPGGDDVDIDFGDVFGGPPKRRSKVTSNEV 60

Query: 61  TRYSFSETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEE-GVHGRRFPSDDFYDDIFK 120
           TR+SFSE+    +L+  D     G   P    +EKPVFGE+     RRF +DDF+DDIF+
Sbjct: 61  TRHSFSES----ALRRRDVIVDVGDLLP---QDEKPVFGEDTSSVRRRFTTDDFFDDIFR 120

Query: 121 GDESVNSSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDL 180
            +ES             SS+PGS++LSPA     P    G+SS P+Q SLP   AK T++
Sbjct: 121 VNES-------------SSLPGSRILSPAH---KPESSSGTSS-PSQFSLP---AKATEI 180

Query: 181 PAFGSSSLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQ 240
           P F  ++ R   S++    T SS   LSR SS                            
Sbjct: 181 PTFNLAATR---SLNKNKETVSSS-PLSRTSS---------------------------- 240

Query: 241 EHGNEEASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLR 300
                      K+D   +  S +   +D  +    G   QFHFSIYKW +KGVP+++   
Sbjct: 241 -----------KADVVSTAKSYSDDCDDPPQVFVTGKGRQFHFSIYKWPNKGVPVVI--- 300

Query: 301 GGNGSRLREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDL 360
                           SS    + KA+     P S  +         +    D + G   
Sbjct: 301 --------------WGSSRLSSMSKAEETTPVPLSDYRKTSVVEKLGKNEEGDGKSGLSG 360

Query: 361 LPDIETVDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVA 420
           L D++    ++      E  +    + +     + + RE        K + S +SE+  +
Sbjct: 361 LKDVKKTSLKRPGVQTKEEKTETDLKSEQAFFGVSKAREAN-----VKPLDSVESEQAFS 420

Query: 421 STTNEDQKHEAKSLSSFLLYSDSEQSEEGIG-KEYRKGEIMAKSDKKSSILSDLSSSPKK 480
             +   +    K L S     D  Q E+ +  +E RKG+  AK+ +  S   D  +  K 
Sbjct: 421 GVSKAHEATTVKPLHSIFHEEDERQDEKIVSEREVRKGKSKAKNTR--SFTEDSRTKKKS 480

Query: 481 QDKQTLKVKKPSPLSSDIESGH---NIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSEND 540
           Q  ++     P P  S   S      +G+  V GK+S+FVK+F++  +       +S   
Sbjct: 481 QGTKSSLDSSPIPDKSSFASSSAAPEVGKDGVKGKVSDFVKIFSKGASVGAGG--ESLGQ 540

Query: 541 SSTMKQESTSK-------AQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAK 600
           SS  + + T K       +  + TVN    D++ K   +  +       S+ +    S +
Sbjct: 541 SSRWRAKETPKTDIIHDGSNAKETVN--IPDQQKKSTPDIPAMNRDQKPSQSTQKKDSDR 600

Query: 601 SAASYRSSFASSKEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNN 660
            + +Y++   + +E    P+T H      +T  D+++PF  NF V+++ +D +K  E N 
Sbjct: 601 ESMNYKAPGDTVQEERQEPSTTH------TTSEDIDEPFHVNFDVEDITQDENKMEEANK 649

Query: 661 GREELQAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQK 720
             EE++ ID KIR+WS+GK GNIRSLLSTLQY+LW  SGWKPVPL+D+IEGNAV++SYQ+
Sbjct: 661 DAEEIKNIDAKIRKWSSGKSGNIRSLLSTLQYILWSGSGWKPVPLMDMIEGNAVRKSYQR 649

Query: 721 ALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLG 745
           ALL LHPDKLQQKGAS++QKY+A KVFE+LQEAW HFNTLG
Sbjct: 721 ALLILHPDKLQQKGASANQKYMAEKVFELLQEAWDHFNTLG 649

BLAST of Lsi04G016420 vs. ExPASy Swiss-Prot
Match: Q0WQ57 (Auxilin-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g12770 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 2.6e-29
Identity = 63/120 (52.50%), Postives = 85/120 (70.83%), Query Frame = 0

Query: 627 SVKELPEDYDKSTETNNGREELQAI----DTKIRQWSNGKEGNIRSLLSTLQYVLWPKSG 686
           + K L E  ++  +    + E   I    D +IR+W  GKEGN+R+LLSTLQYVLWP+ G
Sbjct: 768 AAKALAEKNERDLQVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWPECG 827

Query: 687 WKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNT 743
           W+PV L D+I G +VK+ Y+KA L +HPDK+QQKGA+  QKYIA KVF++L+EAW  FN+
Sbjct: 828 WQPVSLTDLITGASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNS 887

BLAST of Lsi04G016420 vs. ExPASy Swiss-Prot
Match: Q9SU08 (Auxilin-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=AUXI1 PE=1 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 3.7e-28
Identity = 61/120 (50.83%), Postives = 84/120 (70.00%), Query Frame = 0

Query: 627 SVKELPEDYDKSTETNNGREELQAI----DTKIRQWSNGKEGNIRSLLSTLQYVLWPKSG 686
           + K L E  ++  +    + E   I    D +I++W  GKEGN+R+LLSTLQYVLWP+ G
Sbjct: 781 AAKALAEKNERDLQVQREQVEKDRIGVTLDVEIKRWGAGKEGNLRALLSTLQYVLWPECG 840

Query: 687 WKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNT 743
           W+PV L D+I   +VK+ Y+KA L +HPDK+QQKGA+  QKYIA KVF++L+EAW  FN+
Sbjct: 841 WQPVSLTDLITAASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNS 900

BLAST of Lsi04G016420 vs. ExPASy Swiss-Prot
Match: Q9FWS1 (Auxilin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=AUL1 PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.7e-17
Identity = 130/581 (22.38%), Postives = 240/581 (41.31%), Query Frame = 0

Query: 192  SHTNSSRLTLSRFSSSTSSHRF-------EDLKTDYNLPDRTGLLSSEFQE---HGNEEA 251
            SH        S   S+ S  RF       E+   D ++   T   S E ++   +  E  
Sbjct: 900  SHAEMLEYDRSETDSNNSRERFDQTQEQAEETMIDGSIDTDTSRSSFEMRQGDSYIEEVG 959

Query: 252  SSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSRL 311
                +SD     +S     E+ +EE +     Q  +S+ +   +       L+ G  S+ 
Sbjct: 960  IEQHRSDQFPEKASAVSNTEEHIEEID-SDSIQSGWSVVEDDDRS------LQDGGASQA 1019

Query: 312  REKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHE---------RMNVDDEKGT 371
              K          +   +  +E+ +     +N D   + H+         R NV+  + +
Sbjct: 1020 ESK------HDELEETKEESDEMKTSLGVERNGDKKELEHQFECQENETYRSNVEAAESS 1079

Query: 372  DLLPDIETVDQRQSSSALSENLSRQ-------------SSRKDVDSDNIGRPREKEKPHS 431
               P+ E +    ++  + EN   +              +  D+ +   G  +E E   S
Sbjct: 1080 CRFPNGEEIIGAATNGNMKENEGEEGEESCRSSMEEEGDATSDIGAATDGNMKENEGEES 1139

Query: 432  FPKKVSSEKSEKNVASTTNEDQKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDK 491
                +  E         T++  +++A+++   L   D  + +E   +E ++  +M +   
Sbjct: 1140 CRSSMEEE------GDATSDISQNKAETVEEHLKKIDETREKE---RERKQERVMVERAI 1199

Query: 492  KSSILSDLSSSPKKQDKQTLKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTS 551
            + +     + + ++  K  ++  K      ++      G  +V  K+S   K   Q    
Sbjct: 1200 REARERAFADAMERAGKTAMEKAKAVAHRREVPRKSEKGSVEVNDKLSSAEKASMQAKLR 1259

Query: 552  KPRDVVDSENDSSTMKQESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDD 611
              R  V+          E   +A  +A   K    +      +   S +G+     S   
Sbjct: 1260 AERAAVE------RAITEVRERAMEKALSGKSAASQAKSYGGSKSFSSSGERRGSSSSGT 1319

Query: 612  HSAKSAASYRSSFASSKEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKST 671
             +  S  S  S+   + +G P        +  + T     D   E  + K+L +   +  
Sbjct: 1320 ENKSSGPSNSSN--QTAKGEPIQRCKARSERHQRT----SDRAAEALAEKKLRDLKTQKE 1379

Query: 672  ETNNGREELQAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKR 731
            +T   R   +A+D  +++WS+GKE N+R+L+STLQY+L  +SGWKP+PL D++   +V++
Sbjct: 1380 QTERNR-LAEALDADVKRWSSGKENNLRALISTLQYILGAESGWKPIPLTDLVSSASVRK 1439

Query: 732  SYQKALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHF 741
            +Y+KA LY+HPDKLQQ+GAS+ QKYI  KVF++L+EAW  F
Sbjct: 1440 AYRKATLYVHPDKLQQRGASTQQKYICEKVFDLLKEAWNKF 1445

BLAST of Lsi04G016420 vs. ExPASy Swiss-Prot
Match: O13773 (UBA domain-containing protein 7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ucp7 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.6e-08
Identity = 98/396 (24.75%), Postives = 165/396 (41.67%), Query Frame = 0

Query: 391 PREKEKPHSFP--KKVSSEKSEKNVASTTNEDQKHEAKSLSSFLLYSDSEQSEEGIGKEY 450
           P  K KPH  P  +KVS ++   N + + N++      SL  FL       S       Y
Sbjct: 313 PIPKRKPHKVPMNEKVSEDRITTNQSRSGNDE-----SSLVDFLTSKSDNSSFNFPSDTY 372

Query: 451 RKGEIMAKSDKKSSILSDLS------SSPKKQDKQTLKVKKPSPLSSDIESGHNIGRK-K 510
              E + ++  +S+    ++      S+ KK++++TL     + +     +G+ + RK  
Sbjct: 373 EGTENILETAYESTTARQVNNNKPGKSTVKKREEETLNNMVSALVEEQQSTGNELFRKGD 432

Query: 511 VGGKISEFVKLFNQEPTSKPR--------------------------DVVDSENDSSTMK 570
               I EF    +Q P    +                          ++VD   +     
Sbjct: 433 FSQAIEEFTNSLSQLPAKHTKRVPLLSNRSLCYQKVGDLKTCLQDVDELVDIIGEEKGHG 492

Query: 571 QESTSKAQTEATV-NKIRKDEKPKLNRNTDSSIN-------GDDVSKHSMD-DHSAKSAA 630
           +    K+  +  V N +RK +  +       S+N          +SK  +D  H  ++A 
Sbjct: 493 ESIRDKSMNDYYVKNMVRKAQVLEQLEKYQESLNIWKDLIADGQISKLYIDGKHRCEAAI 552

Query: 631 SYRSSFASSKEGSPAPNTV--HVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNG 690
           S  SS + SK  +  P +   H     KS      +  Q     ++  E  D+  E +  
Sbjct: 553 SSHSSESHSKRTTQQPKSTPNHTNIKVKS------ERLQHVRMAQQKAEQLDE--ERSRL 612

Query: 691 REELQAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKA 741
           RE +Q I   + +W  GKE N+R+LL++L  +LWP+  W+ V L +++    VK +Y KA
Sbjct: 613 REPVQQI---VNKWKEGKESNLRALLASLDTILWPECRWQKVSLSELVLPKKVKIAYMKA 672

BLAST of Lsi04G016420 vs. ExPASy TrEMBL
Match: A0A5A7U7E5 (J domain-containing protein required for chloroplast accumulation response 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold234G00360 PE=4 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 631/750 (84.13%), Postives = 677/750 (90.27%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           MEN+SQR+SILLGYSLQRSF N SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MENLSQRDSILLGYSLQRSFAN-SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGGDDEALPGR GPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN
Sbjct: 61  SETGDSFALKGGDDEALPGRGGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPRR   DIFS IPGS+VLSPARPLPPPAEPFGSSSLPAQLSLPSRL KGTDLPAFGSS
Sbjct: 121 SSPRR--GDIFSPIPGSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRLTKGTDLPAFGSS 180

Query: 181 SLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEE 240
           SLRNKD VSNGSHTNS R TLSRFS STSSHRFED KTDY+L DRTG LSS+FQEHG +E
Sbjct: 181 SLRNKDGVSNGSHTNSPRFTLSRFSFSTSSHRFEDPKTDYDLLDRTGALSSKFQEHGGDE 240

Query: 241 ASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSR 300
           A SF KS N LS + LTKG EDSLEESNGGGQFQFHFSIYKWASKGVPL MP R GNG R
Sbjct: 241 ALSFVKSGNGLSGNRLTKGEEDSLEESNGGGQFQFHFSIYKWASKGVPLKMPSR-GNGPR 300

Query: 301 LREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIET 360
           LREKTLLRRSSSSTD +MKAKNE+HSPTSTTQNIDFPP+FHE   VDDEKGTD+LPD++ 
Sbjct: 301 LREKTLLRRSSSSTDMLMKAKNEMHSPTSTTQNIDFPPVFHETTKVDDEKGTDILPDMDN 360

Query: 361 VDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVASTTNED 420
           +++RQSS   SENLSRQSSR  V SDNI  P EK KPHS PKK+SSEKSE+ + S T ED
Sbjct: 361 LEERQSSFTPSENLSRQSSRTAVGSDNISHPIEKAKPHSLPKKISSEKSERKMTSRTIED 420

Query: 421 QKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQT-- 480
           QKHEAKSLSSFLLYSDSEQSEEGI KEYRKGEIMAK D KSS LSDLSSSPKK +KQT  
Sbjct: 421 QKHEAKSLSSFLLYSDSEQSEEGIAKEYRKGEIMAKGDMKSSTLSDLSSSPKKLEKQTSL 480

Query: 481 --LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMKQ 540
              KVKKP+  SSD+ESGHNIGRKKVGGKISEFVKLFNQEPT +P+D VD ENDSSTMKQ
Sbjct: 481 RNSKVKKPTVPSSDMESGHNIGRKKVGGKISEFVKLFNQEPTPRPQDAVDLENDSSTMKQ 540

Query: 541 ESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASSK 600
           ES SKAQ EAT+NKIRKDEK KLN+NTD+S+ GDDVSK S+DD+SAK AAS++S+FASSK
Sbjct: 541 ESESKAQAEATLNKIRKDEKTKLNKNTDASVKGDDVSKKSVDDNSAKKAASFKSNFASSK 600

Query: 601 EGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKIR 660
           + SPAPNTVHVPDV KST+P+VE+PFQ+NFSV+ELP+DY+ +TET NGREE+QA+DTKIR
Sbjct: 601 KSSPAPNTVHVPDVTKSTIPEVEEPFQDNFSVQELPQDYEDATETKNGREEIQALDTKIR 660

Query: 661 QWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720
           QWS+GKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK
Sbjct: 661 QWSSGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720

Query: 721 GASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           GASSDQKYIAAKVFEILQEAW HFNTLGGL
Sbjct: 721 GASSDQKYIAAKVFEILQEAWIHFNTLGGL 746

BLAST of Lsi04G016420 vs. ExPASy TrEMBL
Match: A0A1S3CSZ0 (J domain-containing protein required for chloroplast accumulation response 1 OS=Cucumis melo OX=3656 GN=LOC103503973 PE=4 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 631/750 (84.13%), Postives = 677/750 (90.27%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           MEN+SQR+SILLGYSLQRSF N SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MENLSQRDSILLGYSLQRSFAN-SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGGDDEALPGR GPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN
Sbjct: 61  SETGDSFALKGGDDEALPGRGGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPRR   DIFS IPGS+VLSPARPLPPPAEPFGSSSLPAQLSLPSRL KGTDLPAFGSS
Sbjct: 121 SSPRR--GDIFSPIPGSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRLTKGTDLPAFGSS 180

Query: 181 SLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEE 240
           SLRNKD VSNGSHTNS R TLSRFS STSSHRFED KTDY+L DRTG LSS+FQEHG +E
Sbjct: 181 SLRNKDGVSNGSHTNSPRFTLSRFSFSTSSHRFEDPKTDYDLLDRTGALSSKFQEHGGDE 240

Query: 241 ASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSR 300
           A SF KS N LS + LTKG EDSLEESNGGGQFQFHFSIYKWASKGVPL MP R GNG R
Sbjct: 241 ALSFVKSGNGLSGNRLTKGEEDSLEESNGGGQFQFHFSIYKWASKGVPLKMPSR-GNGPR 300

Query: 301 LREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIET 360
           LREKTLLRRSSSSTD +MKAKNE+HSPTSTTQNIDFPP+FHE   VDDEKGTD+LPD++ 
Sbjct: 301 LREKTLLRRSSSSTDMLMKAKNEMHSPTSTTQNIDFPPVFHETTKVDDEKGTDILPDMDN 360

Query: 361 VDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVASTTNED 420
           +++RQSS   SENLSRQSSR  V SDNI  P EK KPHS PKK+SSEKSE+ + S T ED
Sbjct: 361 LEERQSSFTPSENLSRQSSRTAVGSDNISHPIEKAKPHSLPKKISSEKSERKMTSRTIED 420

Query: 421 QKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQT-- 480
           QKHEAKSLSSFLLYSDSEQSEEGI KEYRKGEIMAK D KSS LSDLSSSPKK +KQT  
Sbjct: 421 QKHEAKSLSSFLLYSDSEQSEEGIAKEYRKGEIMAKGDMKSSTLSDLSSSPKKLEKQTSL 480

Query: 481 --LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMKQ 540
              KVKKP+  SSD+ESGHNIGRKKVGGKISEFVKLFNQEPT +P+D VD ENDSSTMKQ
Sbjct: 481 RNSKVKKPTVPSSDMESGHNIGRKKVGGKISEFVKLFNQEPTPRPQDAVDLENDSSTMKQ 540

Query: 541 ESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASSK 600
           ES SKAQ EAT+NKIRKDEK KLN+NTD+S+ GDDVSK S+DD+SAK AAS++S+FASSK
Sbjct: 541 ESESKAQAEATLNKIRKDEKTKLNKNTDASVKGDDVSKKSVDDNSAKKAASFKSNFASSK 600

Query: 601 EGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKIR 660
           + SPAPNTVHVPDV KST+P+VE+PFQ+NFSV+ELP+DY+ +TET NGREE+QA+DTKIR
Sbjct: 601 KSSPAPNTVHVPDVTKSTIPEVEEPFQDNFSVQELPQDYEDATETKNGREEIQALDTKIR 660

Query: 661 QWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720
           QWS+GKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK
Sbjct: 661 QWSSGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720

Query: 721 GASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           GASSDQKYIAAKVFEILQEAW HFNTLGGL
Sbjct: 721 GASSDQKYIAAKVFEILQEAWIHFNTLGGL 746

BLAST of Lsi04G016420 vs. ExPASy TrEMBL
Match: A0A0A0LIU6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G855300 PE=4 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 622/750 (82.93%), Postives = 667/750 (88.93%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           M+N+SQR+SILLGYSLQRS  N SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MDNLSQRDSILLGYSLQRSSAN-SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGG+DEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN
Sbjct: 61  SETGDSFALKGGEDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPRR   DIFS  PGS+VLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS
Sbjct: 121 SSPRR--GDIFSPNPGSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180

Query: 181 SLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEE 240
           SLRNKDSVSNGSHTNS R TLSRFS STSSHRFED KTDY+L DRTG+L SEFQE+  +E
Sbjct: 181 SLRNKDSVSNGSHTNSPRFTLSRFSFSTSSHRFEDPKTDYDLSDRTGVLPSEFQENDGDE 240

Query: 241 ASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSR 300
           A SF  S N LS +SLTKG EDSLEESNGGGQFQFHFSIYKWASKGVPLMMP R GNG R
Sbjct: 241 ALSFINSGNGLSGNSLTKGEEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPSR-GNGPR 300

Query: 301 LREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIET 360
           LREKTLLR+SSSSTDR++KAKNE+HSPTST QNID  P+FHE   VDDEKG D+LPD   
Sbjct: 301 LREKTLLRKSSSSTDRLVKAKNEMHSPTSTIQNIDISPVFHETTKVDDEKGIDILPDTGN 360

Query: 361 VDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVASTTNED 420
           +DQRQSS   S+NLSRQSSR  V SDNI RP EKEKPHS PKKVSSEK  K + S T ED
Sbjct: 361 LDQRQSSFTPSKNLSRQSSRTAVGSDNISRPTEKEKPHSLPKKVSSEKPAKKMTSRTIED 420

Query: 421 QKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQT-- 480
           QKHEAKSLSSFLLYSDSEQSEE I KEYRKGEIMAK D KSS LSDL SSPKK +KQT  
Sbjct: 421 QKHEAKSLSSFLLYSDSEQSEERITKEYRKGEIMAKGDMKSSNLSDL-SSPKKLEKQTSL 480

Query: 481 --LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMKQ 540
              KVKKP+  SSD+ESGHNIGRKKVGGKISEFVKLFNQEPTSKP+DVVD ENDSSTMKQ
Sbjct: 481 RNSKVKKPTVPSSDVESGHNIGRKKVGGKISEFVKLFNQEPTSKPQDVVDLENDSSTMKQ 540

Query: 541 ESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASSK 600
           ES  K     TVNKIRKDEKPKLN+NTD+SI GD++S+ S+DD+S K AAS++++FASSK
Sbjct: 541 ESEPKG---PTVNKIRKDEKPKLNKNTDASIKGDNISEKSVDDNSTKKAASFKNNFASSK 600

Query: 601 EGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKIR 660
           E SPAPNTVHVP+V KSTV +VE+PFQ+NFSV+ELP+DY+ STETNNGREE+QA+DTKIR
Sbjct: 601 ESSPAPNTVHVPNVTKSTVSEVEEPFQDNFSVQELPQDYEDSTETNNGREEVQALDTKIR 660

Query: 661 QWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720
           QWS+GKEGNIRSLLSTLQYVLWPKSGWK VPLVDIIEGNAVKRSYQKALLYLHPDKLQQK
Sbjct: 661 QWSSGKEGNIRSLLSTLQYVLWPKSGWKAVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720

Query: 721 GASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           GASSDQKYIAAKVFEILQEAW HFNTLGGL
Sbjct: 721 GASSDQKYIAAKVFEILQEAWIHFNTLGGL 742

BLAST of Lsi04G016420 vs. ExPASy TrEMBL
Match: A0A6J1CH28 (J domain-containing protein required for chloroplast accumulation response 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011584 PE=4 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 1.3e-289
Identity = 574/764 (75.13%), Postives = 624/764 (81.68%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           ME +SQRES LLGYSLQRSFTNPSSSPRA  RNSDDVDFHDVFGGPPRRRSSV+E RYSF
Sbjct: 1   MEKLSQRESTLLGYSLQRSFTNPSSSPRAPYRNSDDVDFHDVFGGPPRRRSSVNEARYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           +ET DSF+L+GGD EA      PWSGLNEKPVFGEEG H RRFPSDDFYDDIFKGDES  
Sbjct: 61  TETVDSFALRGGDGEA-----PPWSGLNEKPVFGEEGGHARRFPSDDFYDDIFKGDESAA 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPR   RD+FSSI  S+VLSPARPLPPPAEPFGSSSLPAQLSLPSR AKGTDLPAFGS+
Sbjct: 121 SSPR---RDVFSSISDSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRSAKGTDLPAFGST 180

Query: 181 S---LRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHG 240
           S    RNKDSVSNGSHTNSSR  LSRFSSSTSSHR EDLK+DYN  DRTGLLSSEFQ   
Sbjct: 181 SPNPFRNKDSVSNGSHTNSSRFALSRFSSSTSSHRHEDLKSDYNSSDRTGLLSSEFQHLS 240

Query: 241 NEEASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGN 300
           +EEASSFRKSDN LS +S TK V DSLE+S  GGQFQFHFSIYKWASKGVPLMMPLRGGN
Sbjct: 241 SEEASSFRKSDNTLSGNSSTKEV-DSLEDSTAGGQFQFHFSIYKWASKGVPLMMPLRGGN 300

Query: 301 GSRLREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPD 360
            SRLREK +LRRSSSSTDRV K KNE+ SPTST  NIDFPPIFHE  N++DEKGT LLPD
Sbjct: 301 ASRLREKAMLRRSSSSTDRVAKEKNEMQSPTSTIHNIDFPPIFHETTNLNDEKGTGLLPD 360

Query: 361 IETVDQRQ--------SSSALSENLSRQSSRKDVDSDNI-GRPREKEKPHSFPKKVSSEK 420
           I+  DQR+        +S+A S NLSRQSSRK V SDNI  +  EK +P S  + + SEK
Sbjct: 361 IDAEDQRRPGRSFEQTNSAAPSGNLSRQSSRKGVGSDNIMNQTGEKVRPRSLSETIPSEK 420

Query: 421 SEKNVASTTNEDQKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLS 480
           +EK  A  TNEDQK E KSLSSFLLY+D EQ EEGI KEYRK    AKSDKKSS+ SDLS
Sbjct: 421 AEKKTALITNEDQKQERKSLSSFLLYNDDEQGEEGISKEYRKEVTAAKSDKKSSMPSDLS 480

Query: 481 SSPKKQDKQTL----KVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDV 540
           SSPKKQDK+T     +VKK S  SSD ESGHNIGRKKVGGKISEFVK+FNQEP  K RDV
Sbjct: 481 SSPKKQDKKTSPRNPEVKKSSFQSSDTESGHNIGRKKVGGKISEFVKIFNQEPAPKSRDV 540

Query: 541 VDSENDSSTMKQESTSKAQTEATVNKIRKD--EKPKLNRNTDSSINGDDVSKHSMDDHSA 600
           VDSEND+ST KQE  SKAQ EATV KI KD  EKPKLN NTD+SI  +DV K S+DDHSA
Sbjct: 541 VDSENDTSTRKQEGISKAQIEATVKKISKDEKEKPKLNINTDASIKVNDVLKQSVDDHSA 600

Query: 601 KSAASYRSSFASSKEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETN 660
           K   +Y S+  SSKE S  P  VH+P V+K  VPD+E+PFQENF VKELP++Y++  E  
Sbjct: 601 KK--TYSSN--SSKEDSSVP--VHIPVVSKPKVPDMEEPFQENFMVKELPQEYER--EKV 660

Query: 661 NGREELQAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQ 720
           N  EE +AID KI+QWSNGKEGNIRSLLSTLQ VLWPKSGWKPVPLVDIIEG+AVKRSYQ
Sbjct: 661 NNLEEFRAIDAKIQQWSNGKEGNIRSLLSTLQSVLWPKSGWKPVPLVDIIEGSAVKRSYQ 720

Query: 721 KALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           KALLYLHPDKLQQKGASSDQKYIAAKVF+ILQEAWTHF+T+ GL
Sbjct: 721 KALLYLHPDKLQQKGASSDQKYIAAKVFDILQEAWTHFSTMSGL 747

BLAST of Lsi04G016420 vs. ExPASy TrEMBL
Match: A0A6J1CJL4 (J domain-containing protein required for chloroplast accumulation response 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011584 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.7e-287
Identity = 570/764 (74.61%), Postives = 620/764 (81.15%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           ME +SQRES LLGYSLQRSFTNPSSSPRA  RNSDDVDFHDVFGGPPRRRSSV+E RYSF
Sbjct: 1   MEKLSQRESTLLGYSLQRSFTNPSSSPRAPYRNSDDVDFHDVFGGPPRRRSSVNEARYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           +ET DSF+L+GGD EA      PWSGLNEKPVFGEEG H RRFPSDDFYDDIFKGDES  
Sbjct: 61  TETVDSFALRGGDGEA-----PPWSGLNEKPVFGEEGGHARRFPSDDFYDDIFKGDESAA 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPR   RD+FSSI  S+VLSPARPLPPPAEPFGSSSLPAQLSLPSR AKGTDLPAFGS+
Sbjct: 121 SSPR---RDVFSSISDSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRSAKGTDLPAFGST 180

Query: 181 S---LRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHG 240
           S    RNKDSVSNGSHTNSSR  LSRFSSSTSSHR EDLK+DYN  DRTGLLSSEFQ   
Sbjct: 181 SPNPFRNKDSVSNGSHTNSSRFALSRFSSSTSSHRHEDLKSDYNSSDRTGLLSSEFQHLS 240

Query: 241 NEEASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGN 300
           +EEASSFRKSDN LS +S TK V DSLE+S  GGQFQFHFSIYKWASKGVPLMMPLRGGN
Sbjct: 241 SEEASSFRKSDNTLSGNSSTKEV-DSLEDSTAGGQFQFHFSIYKWASKGVPLMMPLRGGN 300

Query: 301 GSRLREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPD 360
            SRLREK +LRRSSSSTDRV K KNE+ SPTST  NIDFPPIFHE  N++DEKGT LLPD
Sbjct: 301 ASRLREKAMLRRSSSSTDRVAKEKNEMQSPTSTIHNIDFPPIFHETTNLNDEKGTGLLPD 360

Query: 361 IETVDQRQ--------SSSALSENLSRQSSRKDVDSDNI-GRPREKEKPHSFPKKVSSEK 420
           I+  DQR+        +S+A S NLSRQSSRK V SDNI  +  EK +P S  + + SEK
Sbjct: 361 IDAEDQRRPGRSFEQTNSAAPSGNLSRQSSRKGVGSDNIMNQTGEKVRPRSLSETIPSEK 420

Query: 421 SEKNVASTTNEDQKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLS 480
           +EK  A  TNEDQK E KSLSSFLLY+D EQ EEGI KEYRK    AKSDKKSS+ SDLS
Sbjct: 421 AEKKTALITNEDQKQERKSLSSFLLYNDDEQGEEGISKEYRKEVTAAKSDKKSSMPSDLS 480

Query: 481 SSPKKQDKQTL----KVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDV 540
           SSPKKQDK+T     +VKK S  SSD ESGHNIGRKKVGGKISEFVK+FNQEP  K RDV
Sbjct: 481 SSPKKQDKKTSPRNPEVKKSSFQSSDTESGHNIGRKKVGGKISEFVKIFNQEPAPKSRDV 540

Query: 541 VDSENDSSTMKQESTSKAQTEATVNKIRKD--EKPKLNRNTDSSINGDDVSKHSMDDHSA 600
           VDSEND+ST KQE  SKAQ EATV KI KD  EKPKLN NTD+SI  +DV K S+DDHSA
Sbjct: 541 VDSENDTSTRKQEGISKAQIEATVKKISKDEKEKPKLNINTDASIKVNDVLKQSVDDHSA 600

Query: 601 KSAASYRSSFASSKEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETN 660
           K   +Y S+  SSKE S  P  VH+P V+K  VPD+E+PFQENF VKELP++Y++     
Sbjct: 601 KK--TYSSN--SSKEDSSVP--VHIPVVSKPKVPDMEEPFQENFMVKELPQEYERE---- 660

Query: 661 NGREELQAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQ 720
                 +AID KI+QWSNGKEGNIRSLLSTLQ VLWPKSGWKPVPLVDIIEG+AVKRSYQ
Sbjct: 661 ------KAIDAKIQQWSNGKEGNIRSLLSTLQSVLWPKSGWKPVPLVDIIEGSAVKRSYQ 720

Query: 721 KALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           KALLYLHPDKLQQKGASSDQKYIAAKVF+ILQEAWTHF+T+ GL
Sbjct: 721 KALLYLHPDKLQQKGASSDQKYIAAKVFDILQEAWTHFSTMSGL 739

BLAST of Lsi04G016420 vs. NCBI nr
Match: XP_038905516.1 (J domain-containing protein required for chloroplast accumulation response 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 654/751 (87.08%), Postives = 688/751 (91.61%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           MEN+SQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MENLSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGGDDEALPG+ GPWSGLNEKPVFGEEGVHGRRFPS+DFYDDIFKGD+SVN
Sbjct: 61  SETGDSFALKGGDDEALPGQGGPWSGLNEKPVFGEEGVHGRRFPSNDFYDDIFKGDDSVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPRRHERDIFSSIPGS+VLSPARPLPPPAEPFGSSSLPAQLSLPSR+AKGTDLPAFGSS
Sbjct: 121 SSPRRHERDIFSSIPGSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRMAKGTDLPAFGSS 180

Query: 181 SLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEE 240
           SLRNKD V NGSHTNS R TLSRFSSSTSS+RFEDLKTDYNL DRT +LSSE QEHG+E 
Sbjct: 181 SLRNKDVVLNGSHTNSPRFTLSRFSSSTSSNRFEDLKTDYNLSDRTCVLSSESQEHGSEG 240

Query: 241 ASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSR 300
           ASSF+K DNALS +SLTKGVED LEESNGGG FQFHFSIYKWASKGVPLMMPLR GNGSR
Sbjct: 241 ASSFKKPDNALSGNSLTKGVEDGLEESNGGGPFQFHFSIYKWASKGVPLMMPLR-GNGSR 300

Query: 301 LREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIET 360
           LREKTLLR+SSSSTDRV+K+KNE+HSPTST Q IDFPP+FHE   VDDEKGT LLP I+ 
Sbjct: 301 LREKTLLRKSSSSTDRVVKSKNEMHSPTSTIQTIDFPPVFHETTKVDDEKGTGLLPYID- 360

Query: 361 VDQRQSSSALSENLSRQSSRKDVDSD-NIGRPREKEKPHSFPKKVSSEKSEKNVASTTNE 420
           VDQR SSSA SENLSRQSSRKDV  D NI    EKEKPHS P+KV SE SEK + STT E
Sbjct: 361 VDQRLSSSAPSENLSRQSSRKDVGRDNNIDHQTEKEKPHSLPEKVPSESSEKKMISTTIE 420

Query: 421 DQKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQT- 480
           DQK+EAKSLSSFLLYSDSEQSEEGI KEYRKGE MAKSD KSSILSDLSSSPKKQDKQT 
Sbjct: 421 DQKNEAKSLSSFLLYSDSEQSEEGIAKEYRKGETMAKSDMKSSILSDLSSSPKKQDKQTS 480

Query: 481 ---LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMK 540
               KVKKP+P SSD+ESGHNI RKKVGGKISEFVKLFNQEP S+PR+VV+ END+ TMK
Sbjct: 481 LRNSKVKKPTPPSSDMESGHNISRKKVGGKISEFVKLFNQEPASRPREVVNLENDNFTMK 540

Query: 541 QESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASS 600
           QES SKAQTEA VNKIRKDEKPKLN NTD+SI GDDVSK S+DDHS+K  AS RSSFASS
Sbjct: 541 QESASKAQTEAIVNKIRKDEKPKLNSNTDASIKGDDVSKQSVDDHSSKKTASNRSSFASS 600

Query: 601 KEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKI 660
           KE SPAPNTVHVPDVAKSTVPDVE+PFQ+NFSVKELP+DY  S ETNNGREELQAI+TKI
Sbjct: 601 KESSPAPNTVHVPDVAKSTVPDVEEPFQDNFSVKELPQDYSNSAETNNGREELQAIETKI 660

Query: 661 RQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQ 720
           RQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQ
Sbjct: 661 RQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQ 720

Query: 721 KGASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           KGASSDQKYIAAKVFEILQEAWTHFNTLG L
Sbjct: 721 KGASSDQKYIAAKVFEILQEAWTHFNTLGEL 749

BLAST of Lsi04G016420 vs. NCBI nr
Match: XP_038905512.1 (J domain-containing protein required for chloroplast accumulation response 1 isoform X1 [Benincasa hispida] >XP_038905513.1 J domain-containing protein required for chloroplast accumulation response 1 isoform X1 [Benincasa hispida] >XP_038905514.1 J domain-containing protein required for chloroplast accumulation response 1 isoform X1 [Benincasa hispida] >XP_038905515.1 J domain-containing protein required for chloroplast accumulation response 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 653/756 (86.38%), Postives = 687/756 (90.87%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           MEN+SQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MENLSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGGDDEALPG+ GPWSGLNEKPVFGEEGVHGRRFPS+DFYDDIFKGD+SVN
Sbjct: 61  SETGDSFALKGGDDEALPGQGGPWSGLNEKPVFGEEGVHGRRFPSNDFYDDIFKGDDSVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPRRHERDIFSSIPGS+VLSPARPLPPPAEPFGSSSLPAQLSLPSR+AKGTDLPAFGSS
Sbjct: 121 SSPRRHERDIFSSIPGSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRMAKGTDLPAFGSS 180

Query: 181 SLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEE 240
           SLRNKD V NGSHTNS R TLSRFSSSTSS+RFEDLKTDYNL DRT +LSSE QEHG+E 
Sbjct: 181 SLRNKDVVLNGSHTNSPRFTLSRFSSSTSSNRFEDLKTDYNLSDRTCVLSSESQEHGSEG 240

Query: 241 ASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSR 300
           ASSF+K DNALS +SLTKGVED LEESNGGG FQFHFSIYKWASKGVPLMMPLR GNGSR
Sbjct: 241 ASSFKKPDNALSGNSLTKGVEDGLEESNGGGPFQFHFSIYKWASKGVPLMMPLR-GNGSR 300

Query: 301 LREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIET 360
           LREKTLLR+SSSSTDRV+K+KNE+HSPTST Q IDFPP+FHE   VDDEKGT LLP I+ 
Sbjct: 301 LREKTLLRKSSSSTDRVVKSKNEMHSPTSTIQTIDFPPVFHETTKVDDEKGTGLLPYID- 360

Query: 361 VDQRQSSSALSENLSRQSSRKDVDSD-NIGRPREKEKPHSFPKKVSSEKSEKNVASTTNE 420
           VDQR SSSA SENLSRQSSRKDV  D NI    EKEKPHS P+KV SE SEK + STT E
Sbjct: 361 VDQRLSSSAPSENLSRQSSRKDVGRDNNIDHQTEKEKPHSLPEKVPSESSEKKMISTTIE 420

Query: 421 DQKHEAKSLSSFLLYSDSEQ-----SEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQ 480
           DQK+EAKSLSSFLLYSDSEQ      EEGI KEYRKGE MAKSD KSSILSDLSSSPKKQ
Sbjct: 421 DQKNEAKSLSSFLLYSDSEQISFHAGEEGIAKEYRKGETMAKSDMKSSILSDLSSSPKKQ 480

Query: 481 DKQT----LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSEND 540
           DKQT     KVKKP+P SSD+ESGHNI RKKVGGKISEFVKLFNQEP S+PR+VV+ END
Sbjct: 481 DKQTSLRNSKVKKPTPPSSDMESGHNISRKKVGGKISEFVKLFNQEPASRPREVVNLEND 540

Query: 541 SSTMKQESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRS 600
           + TMKQES SKAQTEA VNKIRKDEKPKLN NTD+SI GDDVSK S+DDHS+K  AS RS
Sbjct: 541 NFTMKQESASKAQTEAIVNKIRKDEKPKLNSNTDASIKGDDVSKQSVDDHSSKKTASNRS 600

Query: 601 SFASSKEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQA 660
           SFASSKE SPAPNTVHVPDVAKSTVPDVE+PFQ+NFSVKELP+DY  S ETNNGREELQA
Sbjct: 601 SFASSKESSPAPNTVHVPDVAKSTVPDVEEPFQDNFSVKELPQDYSNSAETNNGREELQA 660

Query: 661 IDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHP 720
           I+TKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHP
Sbjct: 661 IETKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHP 720

Query: 721 DKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           DKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLG L
Sbjct: 721 DKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLGEL 754

BLAST of Lsi04G016420 vs. NCBI nr
Match: XP_008466604.1 (PREDICTED: J domain-containing protein required for chloroplast accumulation response 1 [Cucumis melo] >KAA0051672.1 J domain-containing protein required for chloroplast accumulation response 1 [Cucumis melo var. makuwa] >TYJ97939.1 J domain-containing protein required for chloroplast accumulation response 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 631/750 (84.13%), Postives = 677/750 (90.27%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           MEN+SQR+SILLGYSLQRSF N SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MENLSQRDSILLGYSLQRSFAN-SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGGDDEALPGR GPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN
Sbjct: 61  SETGDSFALKGGDDEALPGRGGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPRR   DIFS IPGS+VLSPARPLPPPAEPFGSSSLPAQLSLPSRL KGTDLPAFGSS
Sbjct: 121 SSPRR--GDIFSPIPGSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRLTKGTDLPAFGSS 180

Query: 181 SLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEE 240
           SLRNKD VSNGSHTNS R TLSRFS STSSHRFED KTDY+L DRTG LSS+FQEHG +E
Sbjct: 181 SLRNKDGVSNGSHTNSPRFTLSRFSFSTSSHRFEDPKTDYDLLDRTGALSSKFQEHGGDE 240

Query: 241 ASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSR 300
           A SF KS N LS + LTKG EDSLEESNGGGQFQFHFSIYKWASKGVPL MP R GNG R
Sbjct: 241 ALSFVKSGNGLSGNRLTKGEEDSLEESNGGGQFQFHFSIYKWASKGVPLKMPSR-GNGPR 300

Query: 301 LREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIET 360
           LREKTLLRRSSSSTD +MKAKNE+HSPTSTTQNIDFPP+FHE   VDDEKGTD+LPD++ 
Sbjct: 301 LREKTLLRRSSSSTDMLMKAKNEMHSPTSTTQNIDFPPVFHETTKVDDEKGTDILPDMDN 360

Query: 361 VDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVASTTNED 420
           +++RQSS   SENLSRQSSR  V SDNI  P EK KPHS PKK+SSEKSE+ + S T ED
Sbjct: 361 LEERQSSFTPSENLSRQSSRTAVGSDNISHPIEKAKPHSLPKKISSEKSERKMTSRTIED 420

Query: 421 QKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQT-- 480
           QKHEAKSLSSFLLYSDSEQSEEGI KEYRKGEIMAK D KSS LSDLSSSPKK +KQT  
Sbjct: 421 QKHEAKSLSSFLLYSDSEQSEEGIAKEYRKGEIMAKGDMKSSTLSDLSSSPKKLEKQTSL 480

Query: 481 --LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMKQ 540
              KVKKP+  SSD+ESGHNIGRKKVGGKISEFVKLFNQEPT +P+D VD ENDSSTMKQ
Sbjct: 481 RNSKVKKPTVPSSDMESGHNIGRKKVGGKISEFVKLFNQEPTPRPQDAVDLENDSSTMKQ 540

Query: 541 ESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASSK 600
           ES SKAQ EAT+NKIRKDEK KLN+NTD+S+ GDDVSK S+DD+SAK AAS++S+FASSK
Sbjct: 541 ESESKAQAEATLNKIRKDEKTKLNKNTDASVKGDDVSKKSVDDNSAKKAASFKSNFASSK 600

Query: 601 EGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKIR 660
           + SPAPNTVHVPDV KST+P+VE+PFQ+NFSV+ELP+DY+ +TET NGREE+QA+DTKIR
Sbjct: 601 KSSPAPNTVHVPDVTKSTIPEVEEPFQDNFSVQELPQDYEDATETKNGREEIQALDTKIR 660

Query: 661 QWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720
           QWS+GKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK
Sbjct: 661 QWSSGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720

Query: 721 GASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           GASSDQKYIAAKVFEILQEAW HFNTLGGL
Sbjct: 721 GASSDQKYIAAKVFEILQEAWIHFNTLGGL 746

BLAST of Lsi04G016420 vs. NCBI nr
Match: XP_004147814.1 (J domain-containing protein required for chloroplast accumulation response 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 622/750 (82.93%), Postives = 667/750 (88.93%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           M+N+SQR+SILLGYSLQRS  N SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MDNLSQRDSILLGYSLQRSSAN-SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGG+DEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN
Sbjct: 61  SETGDSFALKGGEDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180
           SSPRR   DIFS  PGS+VLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS
Sbjct: 121 SSPRR--GDIFSPNPGSRVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDLPAFGSS 180

Query: 181 SLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNEE 240
           SLRNKDSVSNGSHTNS R TLSRFS STSSHRFED KTDY+L DRTG+L SEFQE+  +E
Sbjct: 181 SLRNKDSVSNGSHTNSPRFTLSRFSFSTSSHRFEDPKTDYDLSDRTGVLPSEFQENDGDE 240

Query: 241 ASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGSR 300
           A SF  S N LS +SLTKG EDSLEESNGGGQFQFHFSIYKWASKGVPLMMP R GNG R
Sbjct: 241 ALSFINSGNGLSGNSLTKGEEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPSR-GNGPR 300

Query: 301 LREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIET 360
           LREKTLLR+SSSSTDR++KAKNE+HSPTST QNID  P+FHE   VDDEKG D+LPD   
Sbjct: 301 LREKTLLRKSSSSTDRLVKAKNEMHSPTSTIQNIDISPVFHETTKVDDEKGIDILPDTGN 360

Query: 361 VDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVASTTNED 420
           +DQRQSS   S+NLSRQSSR  V SDNI RP EKEKPHS PKKVSSEK  K + S T ED
Sbjct: 361 LDQRQSSFTPSKNLSRQSSRTAVGSDNISRPTEKEKPHSLPKKVSSEKPAKKMTSRTIED 420

Query: 421 QKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQT-- 480
           QKHEAKSLSSFLLYSDSEQSEE I KEYRKGEIMAK D KSS LSDL SSPKK +KQT  
Sbjct: 421 QKHEAKSLSSFLLYSDSEQSEERITKEYRKGEIMAKGDMKSSNLSDL-SSPKKLEKQTSL 480

Query: 481 --LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMKQ 540
              KVKKP+  SSD+ESGHNIGRKKVGGKISEFVKLFNQEPTSKP+DVVD ENDSSTMKQ
Sbjct: 481 RNSKVKKPTVPSSDVESGHNIGRKKVGGKISEFVKLFNQEPTSKPQDVVDLENDSSTMKQ 540

Query: 541 ESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASSK 600
           ES  K     TVNKIRKDEKPKLN+NTD+SI GD++S+ S+DD+S K AAS++++FASSK
Sbjct: 541 ESEPKG---PTVNKIRKDEKPKLNKNTDASIKGDNISEKSVDDNSTKKAASFKNNFASSK 600

Query: 601 EGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKIR 660
           E SPAPNTVHVP+V KSTV +VE+PFQ+NFSV+ELP+DY+ STETNNGREE+QA+DTKIR
Sbjct: 601 ESSPAPNTVHVPNVTKSTVSEVEEPFQDNFSVQELPQDYEDSTETNNGREEVQALDTKIR 660

Query: 661 QWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720
           QWS+GKEGNIRSLLSTLQYVLWPKSGWK VPLVDIIEGNAVKRSYQKALLYLHPDKLQQK
Sbjct: 661 QWSSGKEGNIRSLLSTLQYVLWPKSGWKAVPLVDIIEGNAVKRSYQKALLYLHPDKLQQK 720

Query: 721 GASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           GASSDQKYIAAKVFEILQEAW HFNTLGGL
Sbjct: 721 GASSDQKYIAAKVFEILQEAWIHFNTLGGL 742

BLAST of Lsi04G016420 vs. NCBI nr
Match: XP_011652403.1 (J domain-containing protein required for chloroplast accumulation response 1 isoform X1 [Cucumis sativus] >KAE8651321.1 hypothetical protein Csa_001755 [Cucumis sativus])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 622/751 (82.82%), Postives = 667/751 (88.81%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60
           M+N+SQR+SILLGYSLQRS  N SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF
Sbjct: 1   MDNLSQRDSILLGYSLQRSSAN-SSSPRASNRNSDDVDFHDVFGGPPRRRSSVHETRYSF 60

Query: 61  SETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120
           SETGDSF+LKGG+DEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN
Sbjct: 61  SETGDSFALKGGEDEALPGRSGPWSGLNEKPVFGEEGVHGRRFPSDDFYDDIFKGDESVN 120

Query: 121 SSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQL-SLPSRLAKGTDLPAFGS 180
           SSPRR   DIFS  PGS+VLSPARPLPPPAEPFGSSSLPAQL SLPSRLAKGTDLPAFGS
Sbjct: 121 SSPRR--GDIFSPNPGSRVLSPARPLPPPAEPFGSSSLPAQLSSLPSRLAKGTDLPAFGS 180

Query: 181 SSLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQEHGNE 240
           SSLRNKDSVSNGSHTNS R TLSRFS STSSHRFED KTDY+L DRTG+L SEFQE+  +
Sbjct: 181 SSLRNKDSVSNGSHTNSPRFTLSRFSFSTSSHRFEDPKTDYDLSDRTGVLPSEFQENDGD 240

Query: 241 EASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLRGGNGS 300
           EA SF  S N LS +SLTKG EDSLEESNGGGQFQFHFSIYKWASKGVPLMMP R GNG 
Sbjct: 241 EALSFINSGNGLSGNSLTKGEEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPSR-GNGP 300

Query: 301 RLREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDLLPDIE 360
           RLREKTLLR+SSSSTDR++KAKNE+HSPTST QNID  P+FHE   VDDEKG D+LPD  
Sbjct: 301 RLREKTLLRKSSSSTDRLVKAKNEMHSPTSTIQNIDISPVFHETTKVDDEKGIDILPDTG 360

Query: 361 TVDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVASTTNE 420
            +DQRQSS   S+NLSRQSSR  V SDNI RP EKEKPHS PKKVSSEK  K + S T E
Sbjct: 361 NLDQRQSSFTPSKNLSRQSSRTAVGSDNISRPTEKEKPHSLPKKVSSEKPAKKMTSRTIE 420

Query: 421 DQKHEAKSLSSFLLYSDSEQSEEGIGKEYRKGEIMAKSDKKSSILSDLSSSPKKQDKQT- 480
           DQKHEAKSLSSFLLYSDSEQSEE I KEYRKGEIMAK D KSS LSDL SSPKK +KQT 
Sbjct: 421 DQKHEAKSLSSFLLYSDSEQSEERITKEYRKGEIMAKGDMKSSNLSDL-SSPKKLEKQTS 480

Query: 481 ---LKVKKPSPLSSDIESGHNIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSENDSSTMK 540
               KVKKP+  SSD+ESGHNIGRKKVGGKISEFVKLFNQEPTSKP+DVVD ENDSSTMK
Sbjct: 481 LRNSKVKKPTVPSSDVESGHNIGRKKVGGKISEFVKLFNQEPTSKPQDVVDLENDSSTMK 540

Query: 541 QESTSKAQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAKSAASYRSSFASS 600
           QES  K     TVNKIRKDEKPKLN+NTD+SI GD++S+ S+DD+S K AAS++++FASS
Sbjct: 541 QESEPKG---PTVNKIRKDEKPKLNKNTDASIKGDNISEKSVDDNSTKKAASFKNNFASS 600

Query: 601 KEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNNGREELQAIDTKI 660
           KE SPAPNTVHVP+V KSTV +VE+PFQ+NFSV+ELP+DY+ STETNNGREE+QA+DTKI
Sbjct: 601 KESSPAPNTVHVPNVTKSTVSEVEEPFQDNFSVQELPQDYEDSTETNNGREEVQALDTKI 660

Query: 661 RQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQ 720
           RQWS+GKEGNIRSLLSTLQYVLWPKSGWK VPLVDIIEGNAVKRSYQKALLYLHPDKLQQ
Sbjct: 661 RQWSSGKEGNIRSLLSTLQYVLWPKSGWKAVPLVDIIEGNAVKRSYQKALLYLHPDKLQQ 720

Query: 721 KGASSDQKYIAAKVFEILQEAWTHFNTLGGL 747
           KGASSDQKYIAAKVFEILQEAW HFNTLGGL
Sbjct: 721 KGASSDQKYIAAKVFEILQEAWIHFNTLGGL 743

BLAST of Lsi04G016420 vs. TAIR 10
Match: AT1G75100.1 (J-domain protein required for chloroplast accumulation response 1 )

HSP 1 Score: 277.7 bits (709), Expect = 2.7e-74
Identity = 253/761 (33.25%), Postives = 372/761 (48.88%), Query Frame = 0

Query: 1   MENMSQRESILLGYSLQRSFTNPSSSP--RASNRNSDDVDFHDVFGGPPRRRSSV---HE 60
           M+ +   E++LLG        + S+ P  R+   +  D+DF DVFGGPP+RRS V     
Sbjct: 1   MQTLPSSETVLLG--------SNSAPPVLRSPGGDDVDIDFGDVFGGPPKRRSKVTSNEV 60

Query: 61  TRYSFSETGDSFSLKGGDDEALPGRSGPWSGLNEKPVFGEE-GVHGRRFPSDDFYDDIFK 120
           TR+SFSE+    +L+  D     G   P    +EKPVFGE+     RRF +DDF+DDIF+
Sbjct: 61  TRHSFSES----ALRRRDVIVDVGDLLP---QDEKPVFGEDTSSVRRRFTTDDFFDDIFR 120

Query: 121 GDESVNSSPRRHERDIFSSIPGSKVLSPARPLPPPAEPFGSSSLPAQLSLPSRLAKGTDL 180
            +ES             SS+PGS++LSPA     P    G+SS P+Q SLP   AK T++
Sbjct: 121 VNES-------------SSLPGSRILSPAH---KPESSSGTSS-PSQFSLP---AKATEI 180

Query: 181 PAFGSSSLRNKDSVSNGSHTNSSRLTLSRFSSSTSSHRFEDLKTDYNLPDRTGLLSSEFQ 240
           P F  ++ R   S++    T SS   LSR SS                            
Sbjct: 181 PTFNLAATR---SLNKNKETVSSS-PLSRTSS---------------------------- 240

Query: 241 EHGNEEASSFRKSDNALSVSSLTKGVEDSLEESNGGGQFQFHFSIYKWASKGVPLMMPLR 300
                      K+D   +  S +   +D  +    G   QFHFSIYKW +KGVP+++   
Sbjct: 241 -----------KADVVSTAKSYSDDCDDPPQVFVTGKGRQFHFSIYKWPNKGVPVVI--- 300

Query: 301 GGNGSRLREKTLLRRSSSSTDRVMKAKNEIHSPTSTTQNIDFPPIFHERMNVDDEKGTDL 360
                           SS    + KA+     P S  +         +    D + G   
Sbjct: 301 --------------WGSSRLSSMSKAEETTPVPLSDYRKTSVVEKLGKNEEGDGKSGLSG 360

Query: 361 LPDIETVDQRQSSSALSENLSRQSSRKDVDSDNIGRPREKEKPHSFPKKVSSEKSEKNVA 420
           L D++    ++      E  +    + +     + + RE        K + S +SE+  +
Sbjct: 361 LKDVKKTSLKRPGVQTKEEKTETDLKSEQAFFGVSKAREAN-----VKPLDSVESEQAFS 420

Query: 421 STTNEDQKHEAKSLSSFLLYSDSEQSEEGIG-KEYRKGEIMAKSDKKSSILSDLSSSPKK 480
             +   +    K L S     D  Q E+ +  +E RKG+  AK+ +  S   D  +  K 
Sbjct: 421 GVSKAHEATTVKPLHSIFHEEDERQDEKIVSEREVRKGKSKAKNTR--SFTEDSRTKKKS 480

Query: 481 QDKQTLKVKKPSPLSSDIESGH---NIGRKKVGGKISEFVKLFNQEPTSKPRDVVDSEND 540
           Q  ++     P P  S   S      +G+  V GK+S+FVK+F++  +       +S   
Sbjct: 481 QGTKSSLDSSPIPDKSSFASSSAAPEVGKDGVKGKVSDFVKIFSKGASVGAGG--ESLGQ 540

Query: 541 SSTMKQESTSK-------AQTEATVNKIRKDEKPKLNRNTDSSINGDDVSKHSMDDHSAK 600
           SS  + + T K       +  + TVN    D++ K   +  +       S+ +    S +
Sbjct: 541 SSRWRAKETPKTDIIHDGSNAKETVN--IPDQQKKSTPDIPAMNRDQKPSQSTQKKDSDR 600

Query: 601 SAASYRSSFASSKEGSPAPNTVHVPDVAKSTVPDVEDPFQENFSVKELPEDYDKSTETNN 660
            + +Y++   + +E    P+T H      +T  D+++PF  NF V+++ +D +K  E N 
Sbjct: 601 ESMNYKAPGDTVQEERQEPSTTH------TTSEDIDEPFHVNFDVEDITQDENKMEEANK 649

Query: 661 GREELQAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQK 720
             EE++ ID KIR+WS+GK GNIRSLLSTLQY+LW  SGWKPVPL+D+IEGNAV++SYQ+
Sbjct: 661 DAEEIKNIDAKIRKWSSGKSGNIRSLLSTLQYILWSGSGWKPVPLMDMIEGNAVRKSYQR 649

Query: 721 ALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLG 745
           ALL LHPDKLQQKGAS++QKY+A KVFE+LQEAW HFNTLG
Sbjct: 721 ALLILHPDKLQQKGASANQKYMAEKVFELLQEAWDHFNTLG 649

BLAST of Lsi04G016420 vs. TAIR 10
Match: AT4G36520.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 9.8e-32
Identity = 58/96 (60.42%), Postives = 81/96 (84.38%), Query Frame = 0

Query: 649  QAIDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYL 708
            + +DT++++WS+GKEGNIR+LLSTLQY+L P+SGW+P+PL ++I   AVKR+Y+KA L +
Sbjct: 1326 ETLDTEVKRWSSGKEGNIRALLSTLQYILGPESGWQPLPLTEVITSAAVKRAYRKATLCV 1385

Query: 709  HPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNTLG 745
            HPDKLQQ+GA+  QKYI  KVF++L+EAW  FN+ G
Sbjct: 1386 HPDKLQQRGANIHQKYICEKVFDLLKEAWNRFNSEG 1421

BLAST of Lsi04G016420 vs. TAIR 10
Match: AT4G12770.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 1.8e-30
Identity = 63/120 (52.50%), Postives = 85/120 (70.83%), Query Frame = 0

Query: 627 SVKELPEDYDKSTETNNGREELQAI----DTKIRQWSNGKEGNIRSLLSTLQYVLWPKSG 686
           + K L E  ++  +    + E   I    D +IR+W  GKEGN+R+LLSTLQYVLWP+ G
Sbjct: 768 AAKALAEKNERDLQVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWPECG 827

Query: 687 WKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNT 743
           W+PV L D+I G +VK+ Y+KA L +HPDK+QQKGA+  QKYIA KVF++L+EAW  FN+
Sbjct: 828 WQPVSLTDLITGASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNS 887

BLAST of Lsi04G016420 vs. TAIR 10
Match: AT4G12770.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 1.8e-30
Identity = 63/120 (52.50%), Postives = 85/120 (70.83%), Query Frame = 0

Query: 627 SVKELPEDYDKSTETNNGREELQAI----DTKIRQWSNGKEGNIRSLLSTLQYVLWPKSG 686
           + K L E  ++  +    + E   I    D +IR+W  GKEGN+R+LLSTLQYVLWP+ G
Sbjct: 766 AAKALAEKNERDLQVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWPECG 825

Query: 687 WKPVPLVDIIEGNAVKRSYQKALLYLHPDKLQQKGASSDQKYIAAKVFEILQEAWTHFNT 743
           W+PV L D+I G +VK+ Y+KA L +HPDK+QQKGA+  QKYIA KVF++L+EAW  FN+
Sbjct: 826 WQPVSLTDLITGASVKKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNS 885

BLAST of Lsi04G016420 vs. TAIR 10
Match: AT4G12780.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 7.0e-30
Identity = 56/92 (60.87%), Postives = 75/92 (81.52%), Query Frame = 0

Query: 651 IDTKIRQWSNGKEGNIRSLLSTLQYVLWPKSGWKPVPLVDIIEGNAVKRSYQKALLYLHP 710
           +D +I++W  GKEGN+R+LLSTLQYVLWP+ GW+PV L D+I   +VK+ Y+KA L +HP
Sbjct: 799 LDVEIKRWGAGKEGNLRALLSTLQYVLWPECGWQPVSLTDLITAASVKKVYRKATLCIHP 858

Query: 711 DKLQQKGASSDQKYIAAKVFEILQEAWTHFNT 743
           DK+QQKGA+  QKYIA KVF++L+EAW  FN+
Sbjct: 859 DKVQQKGANLQQKYIAEKVFDMLKEAWNKFNS 890

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9Q43.8e-7333.25J domain-containing protein required for chloroplast accumulation response 1 OS=... [more]
Q0WQ572.6e-2952.50Auxilin-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g12770 PE=1 SV=1[more]
Q9SU083.7e-2850.83Auxilin-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=AUXI1 PE=1 SV=2[more]
Q9FWS11.7e-1722.38Auxilin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=AUL1 PE=2 SV=2[more]
O137735.6e-0824.75UBA domain-containing protein 7 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
Match NameE-valueIdentityDescription
A0A5A7U7E50.0e+0084.13J domain-containing protein required for chloroplast accumulation response 1 OS=... [more]
A0A1S3CSZ00.0e+0084.13J domain-containing protein required for chloroplast accumulation response 1 OS=... [more]
A0A0A0LIU60.0e+0082.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G855300 PE=4 SV=1[more]
A0A6J1CH281.3e-28975.13J domain-containing protein required for chloroplast accumulation response 1 iso... [more]
A0A6J1CJL42.7e-28774.61J domain-containing protein required for chloroplast accumulation response 1 iso... [more]
Match NameE-valueIdentityDescription
XP_038905516.10.0e+0087.08J domain-containing protein required for chloroplast accumulation response 1 iso... [more]
XP_038905512.10.0e+0086.38J domain-containing protein required for chloroplast accumulation response 1 iso... [more]
XP_008466604.10.0e+0084.13PREDICTED: J domain-containing protein required for chloroplast accumulation res... [more]
XP_004147814.10.0e+0082.93J domain-containing protein required for chloroplast accumulation response 1 iso... [more]
XP_011652403.10.0e+0082.82J domain-containing protein required for chloroplast accumulation response 1 iso... [more]
Match NameE-valueIdentityDescription
AT1G75100.12.7e-7433.25J-domain protein required for chloroplast accumulation response 1 [more]
AT4G36520.19.8e-3260.42Chaperone DnaJ-domain superfamily protein [more]
AT4G12770.11.8e-3052.50Chaperone DnaJ-domain superfamily protein [more]
AT4G12770.21.8e-3052.50Chaperone DnaJ-domain superfamily protein [more]
AT4G12780.27.0e-3060.87Chaperone DnaJ-domain superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 642..745
e-value: 1.6E-38
score: 132.7
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 637..740
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 94..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..204
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 377..426
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 440..459
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 531..546
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 583..601
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 547..561
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 361..645
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 177..204
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 623..645
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 361..376
NoneNo IPR availablePANTHERPTHR23172AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATEDcoord: 1..740
NoneNo IPR availablePANTHERPTHR23172:SF64J DOMAIN-CONTAINING PROTEIN REQUIRED FOR CHLOROPLAST ACCUMULATION RESPONSE 1coord: 1..740

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G016420.1Lsi04G016420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072318 clathrin coat disassembly
biological_process GO:0072583 clathrin-dependent endocytosis
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
cellular_component GO:0031982 vesicle
molecular_function GO:0030276 clathrin binding