Lsi04G016160 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G016160
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cytochrome P450
Location	chr04: 23651418 .. 23654689 (+)
RNA-Seq Expression	Lsi04G016160
Synteny	Lsi04G016160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTCCCTTTCCCAACATATCCACAACCAATCTTGCTCTACCTGGAATAATCTTCACCCTTCTCTTCTTTCTTTATGCTCTCTTCATTTCATTTGGCGTTCGTTGTAAGAGACGGCCGCCCCAACCCGGCGGTGCTTGGCCGATGATCGGCCACCTCCCTCTTCTCACCGGAAAAGAACTCATTCACAATACGTTGGGGAAATTGGCAGACGCTTATGGACCAATCTTTATGCTAAGATTAGGTACCAACAAAACCTTGATTGTTAGTGGCTGGGAAGTAGCAAAGGAGTGCTTTACTACCAACGACAAAAAATTTGCATCTCGCCCCAAATTTGCTGCTGCTAAGCTTCTTGGCTATAACTATGCCATGTTTGGGACTAGCCCATATAGTTCTCATTGGAGACATGTGCGTAAAATAGCCACGCTCGAACTCCTCGCTAACCACCGCCTCGAGAAGCTTCAACACATCCCGAGATTGGAGGTTCAAAGTTCGATTGACAAACTATATGAGTTATGCAGATCAAACAAGAAAGCGTTGGTGGAGATGGAGATGTGGTTTGGTGATATAACACTGAACACCATATTTAGGATGGTAATTGGAAAGCGATTCTCGACTGCTTTTGAAGGGAAGGGTTGGGAAGAGTATCAGAATGCATTGAAGGATTTTTTTTTAATTTGTTTATAGCGTTTCTTCCCTCAGATTTGTTTCCATTTCTAAGATGGTTTGACTTTGGAGGGTACAAGAAAGCCATGAAGAAGACGGCAAAAATCATGGACGAGATGCTTGATAAATGGCTTATAGAGCATCGAGAGAGGAGAAATTCCGATGATGTGGGGATGGAAGAGCAAGATTTCATGCACATCATGTTGTCTATATCCAAGGATGAGGATTTTTCCGACTACGATCTCGATACCGTCATCAAATCTACATGTTTGGTATGTATTAAACTTTAGGAGCATTTACACATATAGCAATCAGATCCAAAATATTAGCAAATATAACACAGTCTAAAAAAATATTGCAAATATAGTAAAATTTGAATTCAGTTATGTGAGTCTATCACAATACAGTTTATCACTGATAGACTTGGCTATATTTACAATTTTTTTTAAAATGCTGCTATAAACTTCATTATTATCCCTAATAGTGCTACTCATTGCCATTACTCTAAATTTAATTTAAAGAAAAATATTTAGTTGCATCTAGACACCAAACCAACTTTATAACTTTTCTTTTTTTAAAAAAAAAATTGAGTGTTTCTTTTTCGTGTAGGTGGTGACGACTAGGGTGGGTTGTGATGTAGTTGATGAGATTTCTCCATTTTTTTTAGACTTTTATATCTGTGCATGTTACAACTTTATATGATTTCTAATACTATATTAGATAAATATGACGTCATCCTATCTCCAAATCGATTAACGATAAGAGTAACTCATGTCTCTTATAAAATTGTGAGATTTTCTATTTTTTTTTTTCTTTCAATAGGAGATGCTTACTTTTAAATAAATTTCTAAAAAAAAAAAAGAAAGAAAAATATTAACATGTTAGGTATGTTCTTTTATTTAAGGAAAATTTTCACAGATAGAAAAAAATATCAAACTATTTAGAAAAACTAGTAAAAAAAAATATTAATAGACTTCTATCAGCGTCTATCACAACTATCTAAAAATTTTGCTATTTTATGTAAATAATTTTCCTTATATTTCTATTTTTAAAAATTCCCCTTTATTTAATGTGGCTAATTAGAATGAGTTGGAAATTATGTGTAGGCTTTGATATTAGGTGGATTTCACACCACCACAGCAGAAATGGTATGGGCTCTATCATTACTAGTGAGTAATGAAGAAGCACTGAAGAAAGTCCAATTAGAGTTGGATGAACAAGTTGGAAGAGACAGAGAAGTGAGAGAATCAGACATGAAAAATTTGATATATCTTCAAGCAGTTGTAAAGGAAACATTGCGTCTTTACCCTGCAGCACAACTGTCAGTCCCTCATGAATGTATAGAAGATTGTACAGTGGCTGGGTATCACATTCCAGCAGGAACAAGATTATGGGTGAATCTTTATAAACTCCAAAGAGATCCAAATGTTTGGGAGAGTCCTACAGAATTTCGTCCAGAAAGGTTTTTGACAAATGAAAAGAGTTTTGATGTGAAAGGACAAACTCCACAATTGATACCATTCGGAGCTGGTAGAAGAATTTGTCCTGGAATTTCATTTGCCATTCAAGTTATGAACTTGTCGCTTGCAAGATTACTTCATGAGTTTGAAATTAGACGACCATCTCAAGACCCACTTAACATGGAAGAAAGCGTTGCGTTGAATATTACCAAGAAAATTCCACTTGAGGTTGTCTTAACTCCACGTCTCTCACCTCAAATTTATAAATGATTTTGTGATAAAATTATTGAAAAAATATATCATTAGTCGGGTTTTTTTTTTTTTTTTAATTTTTTAAAACGCATAGAAAAGTTAGATTAGACCATCTGTACTGTAATATTATATAGGGTGGGTGAAGTGGAGTATTAAACATTTTATATTTTGGTATGTTTTAACCCATTAAAATATGCATGCTCTAATTTGCAATAAAAATACAATATGAATTTTCATCTCAAAATCAATTGGTAGTGAACATGGTTGTTTATGAATTTATAAACACTATGAGATCTTTGATTTACAAAATTTTTAAAGAAAAAAAAATGTTTAAAAATAGAAAAATAAGGAAAACTATTTACATAAAATAGCAAAACTTTAAGATAGTTGTGATAGACGCTTATAGAAGTCTATCAAAGTCTATAAGTGATAGAAATGATAGAATTCATTGATAGATGCTGATAGAAGTTTATCGATGTTTATCAGTGTTTTTTTTTTTTTGCTATTTTCTGTAAATAATTTGATATTTTTTCTAACTGTGAAAATTTTTCAAAAAAATAGGTAGAAGTAATATTTATTGGATTGTTTCAAATATAGAAAAATGAGTTAAAATTATTTACAAATATAGAAAAATTTTACTATCTATCAGCAGCGATAGATCGTGATAAAATTCTATCCGTGATAGATAGTGAAATTTTTTTATATTTATAAATAATTTGATATTTTTTGTTTATAGTAATTTTTCATATTTATTCCCTTATTTTTTTTCAAAATTTAAAACAAAAAATTTAGGTTTTTATGAGGTCTTATTTATTTGGTTGTGTTTGATAATAGACAGACGTGGTTGGGATTATTGGTTCCGGTCGTTGTCAATGCGATCTACTTGTCTGA

mRNA sequence

ATGGATCTTCCCTTTCCCAACATATCCACAACCAATCTTGCTCTACCTGGAATAATCTTCACCCTTCTCTTCTTTCTTTATGCTCTCTTCATTTCATTTGGCGTTCGTTGTAAGAGACGGCCGCCCCAACCCGGCGGTGCTTGGCCGATGATCGGCCACCTCCCTCTTCTCACCGGAAAAGAACTCATTCACAATACGTTGGGGAAATTGGCAGACGCTTATGGACCAATCTTTATGCTAAGATTAGGTACCAACAAAACCTTGATTGTTAGTGGCTGGGAAGTAGCAAAGGAGTGCTTTACTACCAACGACAAAAAATTTGCATCTCGCCCCAAATTTGCTGCTGCTAAGCTTCTTGGCTATAACTATGCCATGTTTGGGACTAGCCCATATAGTTCTCATTGGAGACATGTGCGTAAAATAGCCACGCTCGAACTCCTCGCTAACCACCGCCTCGAGAAGCTTCAACACATCCCGAGATTGGAGGTTCAAAGTTCGATTGACAAACTATATGAGTTATGCAGATCAAACAAGAAAGCGTTGGTGGAGATGGAGATGTGGTTTGGTGATATAACACTGAACACCATATTTAGGATGGTAATTGGAAAGCGATTCTCGACTGCTTTTGAAGGGAAGGCGTTTCTTCCCTCAGATTTGTTTCCATTTCTAAGATGGTTTGACTTTGGAGGGTACAAGAAAGCCATGAAGAAGACGGCAAAAATCATGGACGAGATGCTTGATAAATGGCTTATAGAGCATCGAGAGAGGAGAAATTCCGATGATGTGGTGACGACTAGGGTGGGTTGTGGATTTCACACCACCACAGCAGAAATGGTATGGGCTCTATCATTACTAGTGAGTAATGAAGAAGCACTGAAGAAAGTCCAATTAGAGTTGGATGAACAAGTTGGAAGAGACAGAGAAGTGAGAGAATCAGACATGAAAAATTTGATATATCTTCAAGCAGTTGTAAAGGAAACATTGCGTCTTTACCCTGCAGCACAACTGTCAGTCCCTCATGAATGTATAGAAGATTGTACAGTGGCTGGGTATCACATTCCAGCAGGAACAAGATTATGGGTGAATCTTTATAAACTCCAAAGAGATCCAAATGTTTGGGAGAGTCCTACAGAATTTCGTCCAGAAAGGTTTTTGACAAATGAAAAGAGTTTTGATGTGAAAGGACAAACTCCACAATTGATACCATTCGGAGCTGGTAGAAGAATTTGTCCTGGAATTTCATTTGCCATTCAAGTTATGAACTTGTCGCTTGCAAGATTACTTCATGAGTTTGAAATTAGACGACCATCTCAAGACCCACTTAACATGGAAGAAAGCGTTGCGTTGAATATTACCAAGAAAATTCCACTTGAGACAGACGTGGTTGGGATTATTGGTTCCGGTCGTTGTCAATGCGATCTACTTGTCTGA

Coding sequence (CDS)

ATGGATCTTCCCTTTCCCAACATATCCACAACCAATCTTGCTCTACCTGGAATAATCTTCACCCTTCTCTTCTTTCTTTATGCTCTCTTCATTTCATTTGGCGTTCGTTGTAAGAGACGGCCGCCCCAACCCGGCGGTGCTTGGCCGATGATCGGCCACCTCCCTCTTCTCACCGGAAAAGAACTCATTCACAATACGTTGGGGAAATTGGCAGACGCTTATGGACCAATCTTTATGCTAAGATTAGGTACCAACAAAACCTTGATTGTTAGTGGCTGGGAAGTAGCAAAGGAGTGCTTTACTACCAACGACAAAAAATTTGCATCTCGCCCCAAATTTGCTGCTGCTAAGCTTCTTGGCTATAACTATGCCATGTTTGGGACTAGCCCATATAGTTCTCATTGGAGACATGTGCGTAAAATAGCCACGCTCGAACTCCTCGCTAACCACCGCCTCGAGAAGCTTCAACACATCCCGAGATTGGAGGTTCAAAGTTCGATTGACAAACTATATGAGTTATGCAGATCAAACAAGAAAGCGTTGGTGGAGATGGAGATGTGGTTTGGTGATATAACACTGAACACCATATTTAGGATGGTAATTGGAAAGCGATTCTCGACTGCTTTTGAAGGGAAGGCGTTTCTTCCCTCAGATTTGTTTCCATTTCTAAGATGGTTTGACTTTGGAGGGTACAAGAAAGCCATGAAGAAGACGGCAAAAATCATGGACGAGATGCTTGATAAATGGCTTATAGAGCATCGAGAGAGGAGAAATTCCGATGATGTGGTGACGACTAGGGTGGGTTGTGGATTTCACACCACCACAGCAGAAATGGTATGGGCTCTATCATTACTAGTGAGTAATGAAGAAGCACTGAAGAAAGTCCAATTAGAGTTGGATGAACAAGTTGGAAGAGACAGAGAAGTGAGAGAATCAGACATGAAAAATTTGATATATCTTCAAGCAGTTGTAAAGGAAACATTGCGTCTTTACCCTGCAGCACAACTGTCAGTCCCTCATGAATGTATAGAAGATTGTACAGTGGCTGGGTATCACATTCCAGCAGGAACAAGATTATGGGTGAATCTTTATAAACTCCAAAGAGATCCAAATGTTTGGGAGAGTCCTACAGAATTTCGTCCAGAAAGGTTTTTGACAAATGAAAAGAGTTTTGATGTGAAAGGACAAACTCCACAATTGATACCATTCGGAGCTGGTAGAAGAATTTGTCCTGGAATTTCATTTGCCATTCAAGTTATGAACTTGTCGCTTGCAAGATTACTTCATGAGTTTGAAATTAGACGACCATCTCAAGACCCACTTAACATGGAAGAAAGCGTTGCGTTGAATATTACCAAGAAAATTCCACTTGAGACAGACGTGGTTGGGATTATTGGTTCCGGTCGTTGTCAATGCGATCTACTTGTCTGA

Protein sequence

MDLPFPNISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNKKALVEMEMWFGDITLNTIFRMVIGKRFSTAFEGKAFLPSDLFPFLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDVVTTRVGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEIRRPSQDPLNMEESVALNITKKIPLETDVVGIIGSGRCQCDLLV

Homology

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BLAST of Lsi04G016160 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.5e-118
Identity = 227/512 (44.34%), Postives = 308/512 (60.16%), Query Frame = 0


Query: 1   MDLPFPNISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGK 60
           M+L    ++TT + +  +IF L  FLY          K   P  GGAWP+ GHLPLL G 
Sbjct: 1   MELVLHFLNTTTIGVVSLIFLLCLFLYGPLKKVHGSSK-EAPTVGGAWPIFGHLPLLIGS 60

Query: 61  ELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLG 120
           +  H  LG LA+ +GP+F ++LG  K L+VS WE+A+ECFTTND   ++RPK   A+L+ 
Sbjct: 61  KSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMC 120

Query: 121 YNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNKK- 180
           YN AM   +PY  +WR +RKI   E+L++ R+E+LQ +   EVQ+SI +LY++ RS K  
Sbjct: 121 YNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNE 180

Query: 181 ---ALVEMEMWFGDITLNTIFRMVIGKRF--STAFEGKA----------------FLPSD 240
              A VE++ WF     N + RMV+GKRF  +TA + KA                F   D
Sbjct: 181 SDYASVELKQWFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGD 240

Query: 241 LFPFLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERR--------------------- 300
             P+LRW DFGGY+KAMK+TAK +D M+ +WL EHR++R                     
Sbjct: 241 AIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSLD 300

Query: 301 -------NSD----DVVTTRVGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRD 360
                  ++D      V T +  G   + + ++WA+ L++ N   L+  + ELD QVG+D
Sbjct: 301 GKTIDGIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKD 360

Query: 361 REVRESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKL 420
           R + ESD+ NL+YLQAVVKETLRLY    LS P E  EDCT+ GYH+  GTRL  N++K+
Sbjct: 361 RCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKI 420

Query: 421 QRDPNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLAR 459
             DPNVW  P EF+P+RFLT  K  DVKG   QL+PFG+GRR+CPGISF +Q ++L+LA 
Sbjct: 421 HTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALAS 480

[Close]

BLAST of Lsi04G016160 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.9e-118
Identity = 228/499 (45.69%), Postives = 308/499 (61.72%), Query Frame = 0


Query: 22  LLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKE-LIHNTLGKLADAYGPIFML 81
           L+F   ALF         + P P GAWP+IGHL LL GKE L++ TLGK+AD YGP   L
Sbjct: 13  LVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSL 72

Query: 82  RLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRK 141
           +LG+N+  +VS +EVAK+CFT NDK  ASRP  AAAK +GYN+A+FG +PYS+ WR +RK
Sbjct: 73  QLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRK 132

Query: 142 IATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSN---KKALVEMEMWFGDITLNTIF 201
           IAT+ELL+N RL+ L+H+   E+   +  LY L   N   K  +V+++ W  D+TLN I 
Sbjct: 133 IATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIV 192

Query: 202 RMVIGKRF------------STAFEGK-----------AFLPSDLFPFLRWFDFGGYKKA 261
           RMV GKR+              A + K            F  SD FP L +FD  G++K 
Sbjct: 193 RMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKE 252

Query: 262 MKKTAKIMDEMLDKWLIEHRERR-------NSDDVVTTRVGC------------------ 321
           MK+T   +D +L++W+  HR++R       N  D +   +                    
Sbjct: 253 MKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANTSIK 312

Query: 322 ---------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESDMKNLIYL 381
                    G  T+ + + WA+SLL++N+E LKK Q E+D  VGRDR V +SD++NL+YL
Sbjct: 313 STCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYL 372

Query: 382 QAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPNVWESPTEFR 441
           QA++KETLRLYPA  L  P E +EDCTVAGY++P GTRL VN++K+QRDP V+  P EFR
Sbjct: 373 QAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFR 432

Query: 442 PERFLTNE-KSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEIRRPSQD 459
           PERF+T E K FDV+GQ  +L+PFG+GRR CPG S A+QV++L LAR LH F+++     
Sbjct: 433 PERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDM 492

[Close]

BLAST of Lsi04G016160 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 416.4 bits (1069), Expect = 4.4e-115
Identity = 223/498 (44.78%), Postives = 309/498 (62.05%), Query Frame = 0


Query: 22  LLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKE-LIHNTLGKLADAYGPIFML 81
           L+F   ALF         + P P GAWP+IGHL LL+GKE L++ TLGK+AD YGP   L
Sbjct: 13  LVFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSL 72

Query: 82  RLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRK 141
           RLG+++T +VS +EVAK+CFT NDK  ASRP  AAAK +GY+ A+FG +PYS+ WR +RK
Sbjct: 73  RLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRK 132

Query: 142 IATLELLANHRLEKLQHIPRLEVQSSIDKLYELC---RSNKKALVEMEMWFGDITLNTIF 201
           IATLELL+N RL+ L+H+   E+   +  LY L      ++  +V+++ W  D++LN + 
Sbjct: 133 IATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLNMMV 192

Query: 202 RMVIGKRF--------STAFEGK--------------AFLPSDLFPFLRWFDFGGYKKAM 261
           RMV GKR+          A E +               F  SD FP L WFDF G++K M
Sbjct: 193 RMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEM 252

Query: 262 KKTAKIMDEMLDKWLIEHRERR----------------------------NSDDVVTTRV 321
           K+T + +D +L++W+  HR++R                              D + + + 
Sbjct: 253 KQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITSIKS 312

Query: 322 GC------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESDMKNLIYLQ 381
            C      G  T+ + + WA+SLL++N++ LKK Q E+D  VGRDR V +SD++NL+Y+Q
Sbjct: 313 TCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQ 372

Query: 382 AVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPNVWESPTEFRP 441
           A++KETLRLYPA  L    E IEDCTVAGY++  GTR+ VN++K+QRDP V+  P EFRP
Sbjct: 373 AIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRP 432

Query: 442 ERFLTNE-KSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEIRRPSQDP 459
           ERF+T E K FDV+GQ  +L+PFG+GRR CPG S A+QV++L LAR L  F+++     P
Sbjct: 433 ERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMP 492

[Close]

BLAST of Lsi04G016160 vs. ExPASy Swiss-Prot
Match: O81972 (Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.2e-114
Identity = 225/505 (44.55%), Postives = 295/505 (58.42%), Query Frame = 0


Query: 7   NISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKELIHNT 66
           N +T  + +  +I  L  FL       G   +  PP   GAWP+IGHLPLL G +  H T
Sbjct: 6   NSTTIGVGVVSLILLLYLFLRGGSWKSG---EEGPPTVAGAWPIIGHLPLLLGSKTPHKT 65

Query: 67  LGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMF 126
           LG LAD YGPIF +++G    ++VS WE+AKEC+TTND   +S P   +A LL YN +M 
Sbjct: 66  LGDLADKYGPIFSIKIGAKNAVVVSNWEMAKECYTTNDIAVSSLPDLISANLLCYNRSMI 125

Query: 127 GTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNKK-----AL 186
             +PY  +WR +RKI   E L+  R+E+L H+   EVQSSI +L+   RSNK      A 
Sbjct: 126 VVAPYGPYWRQLRKILMSEFLSPSRVEQLHHVRVSEVQSSITELFRDWRSNKNVQSGFAT 185

Query: 187 VEMEMWFGDITLNTIFRMVIGKRF---STAFEGKA----------------FLPSDLFPF 246
           VE++ WF  +  N I RMV GKR+   ST+ + KA                F   D  P+
Sbjct: 186 VELKQWFSLLVFNMILRMVCGKRYFSASTSDDEKANRCVKAVDEFVRLAATFTVGDAIPY 245

Query: 247 LRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERR------------------------- 306
           LRWFDFGGY+  M++T K +DE++ +WL EHR++R                         
Sbjct: 246 LRWFDFGGYENDMRETGKELDEIIGEWLDEHRQKRKMGENVQDLMSVLLSLLEGKTIEGM 305

Query: 307 NSDDV----VTTRVGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESD 366
           N D V    V T +  G   +   ++WA SL+++N   L+K++ ELD QVG++R + ESD
Sbjct: 306 NVDIVIKSFVLTVIQAGTEASITTLIWATSLILNNPSVLEKLKAELDIQVGKERYICESD 365

Query: 367 MKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPNVW 426
           +  L YLQAVVKETLRLYP A LS P E  EDCT+ GY +  GTRL  NL K+  D NVW
Sbjct: 366 LSKLTYLQAVVKETLRLYPPAPLSRPREFEEDCTIGGYTVKKGTRLITNLSKIHTDHNVW 425

Query: 427 ESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEI 459
            +P EF+PERFLT +K  D+KGQ  QL+PFG GRRICPGI+  +Q + L+LA  LH FEI
Sbjct: 426 SNPLEFKPERFLTTDKDIDMKGQHFQLLPFGGGRRICPGINLGLQTVRLTLASFLHSFEI 485

[Close]

BLAST of Lsi04G016160 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 7.0e-113
Identity = 215/475 (45.26%), Postives = 286/475 (60.21%), Query Frame = 0


Query: 39  RRPPQPGGAWPMIGHLPLLTGKELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKE 98
           + PPQP GAWP+IGHL LL   +L H  L   AD  GP+F ++LG ++ L+V+  E+AKE
Sbjct: 34  KEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKNGPVFKIQLGVHQALVVNNSEIAKE 93

Query: 99  CFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHI 158
           CFTTND+ F +RP   AAK++GYNY M G +PY  +WR +RKI  LE L+N RL+ L+H+
Sbjct: 94  CFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHV 153

Query: 159 PRLEVQSSIDKLYELCRSNK--KALVEMEMWFGDITLNTIFRMVIGKRFSTAFEGKA--- 218
              E+  S  +LY+L  +      LV+M+ W  D+TLN   +MV+GKRF  +    A   
Sbjct: 154 WHSEISISSKELYKLWETQNIDFCLVDMKQWLADLTLNMSVKMVVGKRFFGSASASACEE 213

Query: 219 -------------------FLPSDLFPFLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHR 278
                              F+ SD  P+LRW D GG++K MK+T K +D +   WL EH+
Sbjct: 214 TESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGGHEKEMKRTVKELDILFKGWLDEHK 273

Query: 279 ERRNS------------------------DDVVT-TRVGC------GFHTTTAEMVWALS 338
            +R S                        +DV T  +  C      G  TT A + WALS
Sbjct: 274 RKRLSGGKEDDDQDFMDVMLSILEESKLGNDVDTINKTACLAIILGGADTTWATLTWALS 333

Query: 339 LLVSNEEALKKVQLELDEQVGRDREVRESDMKNLIYLQAVVKETLRLYPAAQLSVPHECI 398
           LL++N  ALKK Q ELD  VGRDR V ESD+  L Y+ A++KETLRLYP   L  P    
Sbjct: 334 LLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVT 393

Query: 399 EDCTVAGYHIPAGTRLWVNLYKLQRDPNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPF 458
           EDCT+AGYH+ AGTRL VN +K+QRDP VW  P E++PERFL  E+  D+KGQ  +LIPF
Sbjct: 394 EDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHEYQPERFL--ERDVDMKGQHFELIPF 453

[Close]

BLAST of Lsi04G016160 vs. ExPASy TrEMBL
Match: A0A5D3BI41 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00520 PE=3 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.8e-211
Identity = 377/512 (73.63%), Postives = 417/512 (81.45%), Query Frame = 0


Query: 1   MDLPFPNISTTNLALPGIIFTLLFFLYALFISF--GVRCKRRPPQPGGAWPMIGHLPLLT 60
           MDLP P IS++NLAL  I+FTLL FLYALF SF  G R  R PPQP GAWP+IGHLPLLT
Sbjct: 1   MDLPLPLISSSNLALTAILFTLLLFLYALFTSFIHGHR-NRLPPQPDGAWPVIGHLPLLT 60

Query: 61  GKELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKL 120
           GKEL+HNTLGK AD YG IFMLRLGTNKTLIVSG EVAKECFTTNDK FASRPKFAAA+L
Sbjct: 61  GKELLHNTLGKFADTYGAIFMLRLGTNKTLIVSGSEVAKECFTTNDKNFASRPKFAAARL 120

Query: 121 LGYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNK 180
           LGYNYAMFG SPY SHWRH+RKI  LELLANHR+ +LQHIPRLEVQ+SI +L+ELCRSNK
Sbjct: 121 LGYNYAMFGVSPYGSHWRHIRKIVMLELLANHRMNRLQHIPRLEVQNSIQELHELCRSNK 180

Query: 181 KALVEMEMWFGDITLNTIFRMVIGKRFSTAFEG-----------------KAFLPSDLFP 240
           K +VEME WFGDITLNTIF+MVIGKRFS AF+G                  AF+PSDLFP
Sbjct: 181 KVVVEMEKWFGDITLNTIFKMVIGKRFSIAFKGIDGEEYQNALKDFFNLFIAFVPSDLFP 240

Query: 241 FLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNS---------------------- 300
           FLRWFDFGGYKK+MKKTAKIMDEM DKWL EHRE+RNS                      
Sbjct: 241 FLRWFDFGGYKKSMKKTAKIMDEMHDKWLREHREKRNSVGVGIEDQDLMHIMLSITMDED 300

Query: 301 ------DDVVTT----RVGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREV 360
                 D ++ +     +  GFHTTTAEMVWALSLL+ +EEALKKVQLELDE+VGR+R+V
Sbjct: 301 FSGYDVDTIIKSTCLAMILGGFHTTTAEMVWALSLLLKHEEALKKVQLELDERVGRERQV 360

Query: 361 RESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRD 420
            ESD+ +LIYLQAVVKETLRLYPAAQLSVPHE IEDCTVAGYH+PAGTRLWVNLYKLQRD
Sbjct: 361 NESDINDLIYLQAVVKETLRLYPAAQLSVPHESIEDCTVAGYHVPAGTRLWVNLYKLQRD 420

Query: 421 PNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLH 462
           PNVWESPTEFRPERFLT+EK++DV GQTP+ IPFG+GRRICPGISFA QVM+L+LARLLH
Sbjct: 421 PNVWESPTEFRPERFLTSEKNYDVNGQTPEFIPFGSGRRICPGISFATQVMHLTLARLLH 480

[Close]

BLAST of Lsi04G016160 vs. ExPASy TrEMBL
Match: A0A1S3CMD1 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502571 PE=3 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.8e-211
Identity = 377/512 (73.63%), Postives = 417/512 (81.45%), Query Frame = 0


Query: 1   MDLPFPNISTTNLALPGIIFTLLFFLYALFISF--GVRCKRRPPQPGGAWPMIGHLPLLT 60
           MDLP P IS++NLAL  I+FTLL FLYALF SF  G R  R PPQP GAWP+IGHLPLLT
Sbjct: 1   MDLPLPLISSSNLALTAILFTLLLFLYALFTSFIHGHR-NRLPPQPDGAWPVIGHLPLLT 60

Query: 61  GKELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKL 120
           GKEL+HNTLGK AD YG IFMLRLGTNKTLIVSG EVAKECFTTNDK FASRPKFAAA+L
Sbjct: 61  GKELLHNTLGKFADTYGAIFMLRLGTNKTLIVSGSEVAKECFTTNDKNFASRPKFAAARL 120

Query: 121 LGYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNK 180
           LGYNYAMFG SPY SHWRH+RKI  LELLANHR+ +LQHIPRLEVQ+SI +L+ELCRSNK
Sbjct: 121 LGYNYAMFGVSPYGSHWRHIRKIVMLELLANHRMNRLQHIPRLEVQNSIQELHELCRSNK 180

Query: 181 KALVEMEMWFGDITLNTIFRMVIGKRFSTAFEG-----------------KAFLPSDLFP 240
           K +VEME WFGDITLNTIF+MVIGKRFS AF+G                  AF+PSDLFP
Sbjct: 181 KVVVEMEKWFGDITLNTIFKMVIGKRFSIAFKGIDGEEYQNALKDFFNLFIAFVPSDLFP 240

Query: 241 FLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNS---------------------- 300
           FLRWFDFGGYKK+MKKTAKIMDEM DKWL EHRE+RNS                      
Sbjct: 241 FLRWFDFGGYKKSMKKTAKIMDEMHDKWLREHREKRNSVGVGIEDQDLMHIMLSITMDED 300

Query: 301 ------DDVVTT----RVGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREV 360
                 D ++ +     +  GFHTTTAEMVWALSLL+ +EEALKKVQLELDE+VGR+R+V
Sbjct: 301 FSGYDVDTIIKSTCLAMILGGFHTTTAEMVWALSLLLKHEEALKKVQLELDERVGRERQV 360

Query: 361 RESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRD 420
            ESD+ +LIYLQAVVKETLRLYPAAQLSVPHE IEDCTVAGYH+PAGTRLWVNLYKLQRD
Sbjct: 361 NESDINDLIYLQAVVKETLRLYPAAQLSVPHESIEDCTVAGYHVPAGTRLWVNLYKLQRD 420

Query: 421 PNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLH 462
           PNVWESPTEFRPERFLT+EK++DV GQTP+ IPFG+GRRICPGISFA QVM+L+LARLLH
Sbjct: 421 PNVWESPTEFRPERFLTSEKNYDVNGQTPEFIPFGSGRRICPGISFATQVMHLTLARLLH 480

[Close]

BLAST of Lsi04G016160 vs. ExPASy TrEMBL
Match: A0A6J1FQP2 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446066 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 4.0e-196
Identity = 349/507 (68.84%), Postives = 394/507 (77.71%), Query Frame = 0


Query: 3   LPFPNISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKEL 62
           +  P+IS+   ALP +IF LL FLYALF  F    KR PPQP GAWP+IGHLPLLT KE 
Sbjct: 1   MEIPHISSPATALP-LIFALLLFLYALFTIFSAHRKRLPPQPRGAWPVIGHLPLLTAKEP 60

Query: 63  IHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYN 122
            H TLGKLAD YGPIFMLRLG N+TLIVSGWEVA+ECFT NDK FASRPKFAAA+LLGY+
Sbjct: 61  NHTTLGKLADVYGPIFMLRLGANRTLIVSGWEVARECFTVNDKAFASRPKFAAAQLLGYD 120

Query: 123 YAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSN---KK 182
           YAMFG SPY SHW H+RKI TLELLANHRLEKL+HIPR EVQSSI KL++LC+S+   KK
Sbjct: 121 YAMFGLSPYGSHWSHLRKIVTLELLANHRLEKLRHIPRSEVQSSIQKLHQLCKSSDGGKK 180

Query: 183 ALVEMEMWFGDITLNTIFRMVIGKRFSTAFEGK-----------------AFLPSDLFPF 242
           ALVEME WFG+I LNTIFRMV+GKRF+TAFEG                   F+PSDLFPF
Sbjct: 181 ALVEMETWFGEIALNTIFRMVVGKRFTTAFEGSGGAEYRHALEDFFNLFTVFVPSDLFPF 240

Query: 243 LRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDVVTTR---------------- 302
           L+WFDFGG+KKAMKKTAK+MDEMLDKWL EHRERRNS +V                    
Sbjct: 241 LKWFDFGGHKKAMKKTAKVMDEMLDKWLTEHRERRNSGEVAPEEQDFMHVMLSFIDTEEF 300

Query: 303 ----------------VGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVR 362
                           +  GFHTTT EM WALSLL++ EEA +KVQ ELDE VGRDR V+
Sbjct: 301 SGYDADTIIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKVQEELDEHVGRDRVVK 360

Query: 363 ESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDP 422
           ESD+K LIYLQAVVKETLR+YPAAQLSVPHE  EDCT+AGYHIPA TRLWVNLYKLQRDP
Sbjct: 361 ESDLKILIYLQAVVKETLRMYPAAQLSVPHESTEDCTIAGYHIPAKTRLWVNLYKLQRDP 420

Query: 423 NVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHE 458
           +VWE+P +F PERFLT +K+FDVKGQ PQLIPFGAGRRICP + FA+QV++LS ARLLHE
Sbjct: 421 SVWENPDQFCPERFLTTQKNFDVKGQNPQLIPFGAGRRICPAMIFALQVLHLSFARLLHE 480

[Close]

BLAST of Lsi04G016160 vs. ExPASy TrEMBL
Match: A0A6J1FPF7 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446067 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.7e-194
Identity = 344/508 (67.72%), Postives = 393/508 (77.36%), Query Frame = 0


Query: 3   LPFPNISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKEL 62
           +  P IS+   +LP  IF LL FLYALF       KR PPQP GAWP+IGHLPLLT KE 
Sbjct: 1   MELPQISSPTTSLPA-IFALLLFLYALFTIISAHRKRLPPQPRGAWPVIGHLPLLTAKEP 60

Query: 63  IHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYN 122
            H TLGKLAD YGPIFMLRLG ++TLIVSGWEVA+ECFT NDK FASRPKFAAA+LLGY+
Sbjct: 61  NHTTLGKLADVYGPIFMLRLGVHRTLIVSGWEVARECFTVNDKAFASRPKFAAAQLLGYD 120

Query: 123 YAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSN---KK 182
           YAMFG SPY SHW H+RKIATLELLANHRLEKL+HIPR EVQSSI KL++LC+S+   KK
Sbjct: 121 YAMFGLSPYGSHWSHLRKIATLELLANHRLEKLRHIPRSEVQSSIQKLHQLCKSSDGGKK 180

Query: 183 ALVEMEMWFGDITLNTIFRMVIGKRFSTAFEGK-----------------AFLPSDLFPF 242
           ALVEME WFG+I LNTIFRMV+GKRF+TAFEG                   F+PSDLFPF
Sbjct: 181 ALVEMETWFGEIALNTIFRMVVGKRFTTAFEGSGGAEYRHALEDFFNLFTVFVPSDLFPF 240

Query: 243 LRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDV-------------------- 302
           L+WFDFGG+KKAMKKTAK+MDEMLDKWL EHRERRNS +V                    
Sbjct: 241 LKWFDFGGHKKAMKKTAKVMDEMLDKWLTEHRERRNSGEVAPEEQDFMHVMLSFIDNEEF 300

Query: 303 ------VTTRVGC------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVR 362
                    +  C      GFHTTT EM WALSLL++ EEA +K+Q ELD+ VGRDR VR
Sbjct: 301 SGYDADTVIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKLQKELDQHVGRDRVVR 360

Query: 363 ESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDP 422
           ESD+KNL+YLQAVVKETLR+YPA QLSVPHE IEDCTVAGYHIPA TRLWVNLY+LQRDP
Sbjct: 361 ESDLKNLVYLQAVVKETLRMYPAGQLSVPHESIEDCTVAGYHIPAKTRLWVNLYRLQRDP 420

Query: 423 NVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHE 459
           +VWE+P EF PERFLT +K+FDVKG+ PQLIPFG+GRRICP ++FA+Q+++LS ARLLHE
Sbjct: 421 SVWENPNEFFPERFLTTQKNFDVKGRNPQLIPFGSGRRICPAVTFALQILHLSFARLLHE 480

[Close]

BLAST of Lsi04G016160 vs. ExPASy TrEMBL
Match: A0A6J1FIL1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446066 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 8.4e-194
Identity = 344/507 (67.85%), Postives = 394/507 (77.71%), Query Frame = 0


Query: 3   LPFPNISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKEL 62
           +  P+IS+T +ALP  IF LLFFLYALF  F    KR PPQP GAWP+IGHLPLL+GKE 
Sbjct: 1   MELPHISSTTIALPA-IFALLFFLYALFTIFSAHRKRLPPQPRGAWPVIGHLPLLSGKEP 60

Query: 63  IHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYN 122
            H  LGKLAD YGPIFMLRLG ++TLIVSGWEVA+EC T NDK FASRPKFAAA+LLGY+
Sbjct: 61  HHTALGKLADVYGPIFMLRLGAHRTLIVSGWEVARECLTVNDKAFASRPKFAAAQLLGYD 120

Query: 123 YAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSN---KK 182
           YAMF  SPY SHW H+RKIATLELLANHRLEKLQHIPR EVQ SI+KL++ C+SN    K
Sbjct: 121 YAMFALSPYGSHWSHLRKIATLELLANHRLEKLQHIPRSEVQISIEKLHQFCKSNNGGNK 180

Query: 183 ALVEMEMWFGDITLNTIFRMVIGKRFSTAFEGK-----------------AFLPSDLFPF 242
           AL+EME WFG++ LNTIFRMV+GKRF+TAFEG                  AF+PSDLF F
Sbjct: 181 ALMEMETWFGEMALNTIFRMVVGKRFTTAFEGSGGAEYRHALEDFFNLFTAFVPSDLFAF 240

Query: 243 LRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDV-------------------- 302
           L+WFDFGG+KKAMKKTAK++D+MLDKWL EHRERRNS +V                    
Sbjct: 241 LKWFDFGGHKKAMKKTAKVLDKMLDKWLAEHRERRNSGEVTPEEQDFMHVMLSFIDNEEF 300

Query: 303 ------VTTRVGC------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVR 362
                    +  C      GFHTTT EM WALSLL++ EEA +KVQ ELDE VGRDR V+
Sbjct: 301 PGYDADTVIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKVQEELDEHVGRDRVVK 360

Query: 363 ESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDP 422
           ESD+K LIYLQAVVKETLR+YPAAQLSVPHE  EDCT+AGYHIPA TRLWVNLYKLQRDP
Sbjct: 361 ESDLKILIYLQAVVKETLRMYPAAQLSVPHESTEDCTIAGYHIPAKTRLWVNLYKLQRDP 420

Query: 423 NVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHE 458
           +VWE+P +F PERFLT +K+FDVKGQ PQLIPFGAGRRICP + FA+QV++LS ARLLHE
Sbjct: 421 SVWENPDQFCPERFLTTQKNFDVKGQNPQLIPFGAGRRICPAMIFALQVLHLSFARLLHE 480

[Close]

BLAST of Lsi04G016160 vs. NCBI nr
Match: XP_038898814.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 760.4 bits (1962), Expect = 9.4e-216
Identity = 385/507 (75.94%), Postives = 416/507 (82.05%), Query Frame = 0


Query: 1   MDLPFPNISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGK 60
           MDL  P+IS++NLA P II TLLFFLYAL I F  R  R PPQPGGAWP+IGHLPLLTGK
Sbjct: 1   MDLLLPHISSSNLAQPAIILTLLFFLYALLILFRGRHTRLPPQPGGAWPVIGHLPLLTGK 60

Query: 61  ELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLG 120
           ELIHNTLGKLADAYGPIFMLRLG+NKTLIVSGWEVAKECFTTNDKKFASRPKFAAA+LLG
Sbjct: 61  ELIHNTLGKLADAYGPIFMLRLGSNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAELLG 120

Query: 121 YNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNKKA 180
           YNYAMFGTSPY SHWRHVRKI  LELLANHRLEKLQHIPR EVQSSI+ L+ELCRSNKKA
Sbjct: 121 YNYAMFGTSPYGSHWRHVRKIVMLELLANHRLEKLQHIPRSEVQSSIETLHELCRSNKKA 180

Query: 181 LVEMEMWFGDITLNTIFRMVIGKRFSTAFEGK-----------------AFLPSDLFPFL 240
           LVEME WFGDITLN+IFRMV+GKRFSTAFE                     +PSDLFPFL
Sbjct: 181 LVEMETWFGDITLNSIFRMVVGKRFSTAFEESGGEKYHNAMKDFFNLFTVLVPSDLFPFL 240

Query: 241 RWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDV-------------VTTR---- 300
           RWFDFGGYKK MK+TAK+MDEML KWL EHRE+RNSD V             +T      
Sbjct: 241 RWFDFGGYKKTMKETAKVMDEMLHKWLQEHREKRNSDGVGMEEQDFMHVMLSITEEEDFS 300

Query: 301 ---------------VGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRE 360
                          +  G+HTTT EMVWALSLLV+NEE+LKKVQ ELDEQVGR+REVRE
Sbjct: 301 GYDIDNVIKSTCLAVILGGYHTTTTEMVWALSLLVNNEESLKKVQFELDEQVGREREVRE 360

Query: 361 SDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPN 420
           SDM NLIYLQAVVKETLRLYPAAQLSVPHE IEDCTVAGYHI AGTRLWVNLYKLQRDP 
Sbjct: 361 SDMNNLIYLQAVVKETLRLYPAAQLSVPHESIEDCTVAGYHIRAGTRLWVNLYKLQRDPC 420

Query: 421 VWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEF 459
           VWESP EFRPERFLT+EK+FDVKGQ+PQ IPFG GRRICPGISFA++VM+LSLARLLHEF
Sbjct: 421 VWESPREFRPERFLTSEKNFDVKGQSPQFIPFGVGRRICPGISFAMKVMHLSLARLLHEF 480

[Close]

BLAST of Lsi04G016160 vs. NCBI nr
Match: XP_038897312.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 388/509 (76.23%), Postives = 417/509 (81.93%), Query Frame = 0


Query: 1   MDLPFPNISTTNLALPGIIFT-LLFFLYALFISF-GVRCKRRPPQPGGAWPMIGHLPLLT 60
           M+LP  ++S++NLALP IIFT LL  LYALF SF G R KR PPQPGGAWP+IGHLPLLT
Sbjct: 1   MNLPLADMSSSNLALPAIIFTLLLIILYALFNSFIGGRHKRLPPQPGGAWPVIGHLPLLT 60

Query: 61  GKELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKL 120
           GKELIHNTLGKLAD YGPIFMLRLGTNKTL+VSGWEVAKECFTTNDK FASRPKFAAA+L
Sbjct: 61  GKELIHNTLGKLADTYGPIFMLRLGTNKTLVVSGWEVAKECFTTNDKIFASRPKFAAAEL 120

Query: 121 LGYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNK 180
           LGYNYAMFGTSPY SHWRHVRKI  LELLANHRLEKLQHI R EVQ+SI++L+ELC+SNK
Sbjct: 121 LGYNYAMFGTSPYGSHWRHVRKIVMLELLANHRLEKLQHISRSEVQNSIERLHELCKSNK 180

Query: 181 KALVEMEMWFGDITLNTIFRMVIGKRFSTAFEGK-----------------AFLPSDLFP 240
           KALVEME WFGDITLNTIFRMV+GKRFSTAFEG                  AF+PSDLFP
Sbjct: 181 KALVEMETWFGDITLNTIFRMVVGKRFSTAFEGSGGEEYQNALKGFFNLFTAFVPSDLFP 240

Query: 241 FLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDV------------------- 300
           FLRWFDFGGYKK MKKTAK+MDEM DKWL EHRE+RN D V                   
Sbjct: 241 FLRWFDFGGYKKTMKKTAKVMDEMHDKWLREHREKRNFDGVGSEEQDLMHIMLSITKDED 300

Query: 301 -------VTTRVGC------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREV 360
                     +  C      GFHTTT EMVWALSLL++NEEALKKVQ ELDE VGR+R+V
Sbjct: 301 FSGYDVDTVIKSTCLAMILGGFHTTTVEMVWALSLLLNNEEALKKVQSELDELVGRERQV 360

Query: 361 RESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRD 420
            ESD+ +LIYLQAVVKETLRLYPAAQLSVPHE IEDCTVAGYHIPAGTRLWVNLYKLQ D
Sbjct: 361 SESDIHDLIYLQAVVKETLRLYPAAQLSVPHESIEDCTVAGYHIPAGTRLWVNLYKLQSD 420

Query: 421 PNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLH 459
           PNVWESP EFRPERFL  EK+FDVKGQ+PQ IPFGAGRRICPGISFA QVM LSLARLLH
Sbjct: 421 PNVWESPREFRPERFLAIEKNFDVKGQSPQFIPFGAGRRICPGISFATQVMQLSLARLLH 480

[Close]

BLAST of Lsi04G016160 vs. NCBI nr
Match: XP_004147802.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KAE8651314.1 hypothetical protein Csa_001976 [Cucumis sativus])

HSP 1 Score: 752.3 bits (1941), Expect = 2.6e-213
Identity = 376/511 (73.58%), Postives = 417/511 (81.60%), Query Frame = 0


Query: 1   MDLPFPNISTTNLALPGIIFTLLFFLYALFISF-GVRCKRRPPQPGGAWPMIGHLPLLTG 60
           MDLP   IS++N ALP I+FTLLFFLYALF SF   R  R PPQP GAWP+IGHLPLLTG
Sbjct: 1   MDLPLLLISSSNFALPAILFTLLFFLYALFASFIHGRRNRLPPQPDGAWPVIGHLPLLTG 60

Query: 61  KELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLL 120
           KEL+HNTLGK AD YGPIFMLR GT KTLIVSGWEVAKECFTTNDK FASRPKFAAA+LL
Sbjct: 61  KELLHNTLGKFADNYGPIFMLRFGTKKTLIVSGWEVAKECFTTNDKIFASRPKFAAAQLL 120

Query: 121 GYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNKK 180
           GYNYAMFGTSPY SHWRH+RKI  LELLANHR+ +LQHIPRLEVQ+SI++L+ELCRSNKK
Sbjct: 121 GYNYAMFGTSPYGSHWRHIRKIVMLELLANHRMNRLQHIPRLEVQNSIEELHELCRSNKK 180

Query: 181 ALVEMEMWFGDITLNTIFRMVIGKRFSTAFEG-----------------KAFLPSDLFPF 240
           A+VEME WFGDITLNTIF+MVIGKRFST FEG                  AF+PSDLFPF
Sbjct: 181 AVVEMEKWFGDITLNTIFKMVIGKRFSTTFEGCHGEEYQNALKDFFNLFIAFVPSDLFPF 240

Query: 241 LRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDVV------------------- 300
           LRWFDFGGYKK+MKKTAKIMDEM DKWL EHRE+RNSD +V                   
Sbjct: 241 LRWFDFGGYKKSMKKTAKIMDEMHDKWLREHREKRNSDGLVIEDQDLMDIMLSITKDEDF 300

Query: 301 -------------TTRVGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVR 360
                           +  GFHTTTA+MVWALSLL+ +EEALKKVQLELDE+VGR+R+V 
Sbjct: 301 SGYDVDTIIKSTCLAMILGGFHTTTAQMVWALSLLLKHEEALKKVQLELDERVGRERQVN 360

Query: 361 ESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDP 420
           ESD+ +LIYLQAVVKE LRLYPAAQLSVPHE IEDCTVAGYH+PAGTRLWVNLYKLQRDP
Sbjct: 361 ESDINDLIYLQAVVKEALRLYPAAQLSVPHESIEDCTVAGYHVPAGTRLWVNLYKLQRDP 420

Query: 421 NVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHE 462
           NVWESPTEFRPERFLT+EK++DV GQTP+ IPFG+GRRICPGISFAIQVM+L+LARLLHE
Sbjct: 421 NVWESPTEFRPERFLTSEKNYDVNGQTPEFIPFGSGRRICPGISFAIQVMHLTLARLLHE 480

[Close]

BLAST of Lsi04G016160 vs. NCBI nr
Match: XP_008464764.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032715.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98746.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 743.4 bits (1918), Expect = 1.2e-210
Identity = 377/512 (73.63%), Postives = 417/512 (81.45%), Query Frame = 0


Query: 1   MDLPFPNISTTNLALPGIIFTLLFFLYALFISF--GVRCKRRPPQPGGAWPMIGHLPLLT 60
           MDLP P IS++NLAL  I+FTLL FLYALF SF  G R  R PPQP GAWP+IGHLPLLT
Sbjct: 1   MDLPLPLISSSNLALTAILFTLLLFLYALFTSFIHGHR-NRLPPQPDGAWPVIGHLPLLT 60

Query: 61  GKELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKL 120
           GKEL+HNTLGK AD YG IFMLRLGTNKTLIVSG EVAKECFTTNDK FASRPKFAAA+L
Sbjct: 61  GKELLHNTLGKFADTYGAIFMLRLGTNKTLIVSGSEVAKECFTTNDKNFASRPKFAAARL 120

Query: 121 LGYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNK 180
           LGYNYAMFG SPY SHWRH+RKI  LELLANHR+ +LQHIPRLEVQ+SI +L+ELCRSNK
Sbjct: 121 LGYNYAMFGVSPYGSHWRHIRKIVMLELLANHRMNRLQHIPRLEVQNSIQELHELCRSNK 180

Query: 181 KALVEMEMWFGDITLNTIFRMVIGKRFSTAFEG-----------------KAFLPSDLFP 240
           K +VEME WFGDITLNTIF+MVIGKRFS AF+G                  AF+PSDLFP
Sbjct: 181 KVVVEMEKWFGDITLNTIFKMVIGKRFSIAFKGIDGEEYQNALKDFFNLFIAFVPSDLFP 240

Query: 241 FLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNS---------------------- 300
           FLRWFDFGGYKK+MKKTAKIMDEM DKWL EHRE+RNS                      
Sbjct: 241 FLRWFDFGGYKKSMKKTAKIMDEMHDKWLREHREKRNSVGVGIEDQDLMHIMLSITMDED 300

Query: 301 ------DDVVTT----RVGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREV 360
                 D ++ +     +  GFHTTTAEMVWALSLL+ +EEALKKVQLELDE+VGR+R+V
Sbjct: 301 FSGYDVDTIIKSTCLAMILGGFHTTTAEMVWALSLLLKHEEALKKVQLELDERVGRERQV 360

Query: 361 RESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRD 420
            ESD+ +LIYLQAVVKETLRLYPAAQLSVPHE IEDCTVAGYH+PAGTRLWVNLYKLQRD
Sbjct: 361 NESDINDLIYLQAVVKETLRLYPAAQLSVPHESIEDCTVAGYHVPAGTRLWVNLYKLQRD 420

Query: 421 PNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLH 462
           PNVWESPTEFRPERFLT+EK++DV GQTP+ IPFG+GRRICPGISFA QVM+L+LARLLH
Sbjct: 421 PNVWESPTEFRPERFLTSEKNYDVNGQTPEFIPFGSGRRICPGISFATQVMHLTLARLLH 480

[Close]

BLAST of Lsi04G016160 vs. NCBI nr
Match: XP_022940480.1 (cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 694.1 bits (1790), Expect = 8.3e-196
Identity = 349/507 (68.84%), Postives = 394/507 (77.71%), Query Frame = 0


Query: 3   LPFPNISTTNLALPGIIFTLLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKEL 62
           +  P+IS+   ALP +IF LL FLYALF  F    KR PPQP GAWP+IGHLPLLT KE 
Sbjct: 1   MEIPHISSPATALP-LIFALLLFLYALFTIFSAHRKRLPPQPRGAWPVIGHLPLLTAKEP 60

Query: 63  IHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYN 122
            H TLGKLAD YGPIFMLRLG N+TLIVSGWEVA+ECFT NDK FASRPKFAAA+LLGY+
Sbjct: 61  NHTTLGKLADVYGPIFMLRLGANRTLIVSGWEVARECFTVNDKAFASRPKFAAAQLLGYD 120

Query: 123 YAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSN---KK 182
           YAMFG SPY SHW H+RKI TLELLANHRLEKL+HIPR EVQSSI KL++LC+S+   KK
Sbjct: 121 YAMFGLSPYGSHWSHLRKIVTLELLANHRLEKLRHIPRSEVQSSIQKLHQLCKSSDGGKK 180

Query: 183 ALVEMEMWFGDITLNTIFRMVIGKRFSTAFEGK-----------------AFLPSDLFPF 242
           ALVEME WFG+I LNTIFRMV+GKRF+TAFEG                   F+PSDLFPF
Sbjct: 181 ALVEMETWFGEIALNTIFRMVVGKRFTTAFEGSGGAEYRHALEDFFNLFTVFVPSDLFPF 240

Query: 243 LRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSDDVVTTR---------------- 302
           L+WFDFGG+KKAMKKTAK+MDEMLDKWL EHRERRNS +V                    
Sbjct: 241 LKWFDFGGHKKAMKKTAKVMDEMLDKWLTEHRERRNSGEVAPEEQDFMHVMLSFIDTEEF 300

Query: 303 ----------------VGCGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVR 362
                           +  GFHTTT EM WALSLL++ EEA +KVQ ELDE VGRDR V+
Sbjct: 301 SGYDADTIIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKVQEELDEHVGRDRVVK 360

Query: 363 ESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDP 422
           ESD+K LIYLQAVVKETLR+YPAAQLSVPHE  EDCT+AGYHIPA TRLWVNLYKLQRDP
Sbjct: 361 ESDLKILIYLQAVVKETLRMYPAAQLSVPHESTEDCTIAGYHIPAKTRLWVNLYKLQRDP 420

Query: 423 NVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHE 458
           +VWE+P +F PERFLT +K+FDVKGQ PQLIPFGAGRRICP + FA+QV++LS ARLLHE
Sbjct: 421 SVWENPDQFCPERFLTTQKNFDVKGQNPQLIPFGAGRRICPAMIFALQVLHLSFARLLHE 480

[Close]

BLAST of Lsi04G016160 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 427.6 bits (1098), Expect = 1.4e-119
Identity = 228/499 (45.69%), Postives = 308/499 (61.72%), Query Frame = 0


Query: 22  LLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKE-LIHNTLGKLADAYGPIFML 81
           L+F   ALF         + P P GAWP+IGHL LL GKE L++ TLGK+AD YGP   L
Sbjct: 13  LVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSL 72

Query: 82  RLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRK 141
           +LG+N+  +VS +EVAK+CFT NDK  ASRP  AAAK +GYN+A+FG +PYS+ WR +RK
Sbjct: 73  QLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRK 132

Query: 142 IATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSN---KKALVEMEMWFGDITLNTIF 201
           IAT+ELL+N RL+ L+H+   E+   +  LY L   N   K  +V+++ W  D+TLN I 
Sbjct: 133 IATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIV 192

Query: 202 RMVIGKRF------------STAFEGK-----------AFLPSDLFPFLRWFDFGGYKKA 261
           RMV GKR+              A + K            F  SD FP L +FD  G++K 
Sbjct: 193 RMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKE 252

Query: 262 MKKTAKIMDEMLDKWLIEHRERR-------NSDDVVTTRVGC------------------ 321
           MK+T   +D +L++W+  HR++R       N  D +   +                    
Sbjct: 253 MKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANTSIK 312

Query: 322 ---------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESDMKNLIYL 381
                    G  T+ + + WA+SLL++N+E LKK Q E+D  VGRDR V +SD++NL+YL
Sbjct: 313 STCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYL 372

Query: 382 QAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPNVWESPTEFR 441
           QA++KETLRLYPA  L  P E +EDCTVAGY++P GTRL VN++K+QRDP V+  P EFR
Sbjct: 373 QAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFR 432

Query: 442 PERFLTNE-KSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEIRRPSQD 459
           PERF+T E K FDV+GQ  +L+PFG+GRR CPG S A+QV++L LAR LH F+++     
Sbjct: 433 PERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDM 492

[Close]

BLAST of Lsi04G016160 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 416.4 bits (1069), Expect = 3.1e-116
Identity = 223/498 (44.78%), Postives = 309/498 (62.05%), Query Frame = 0


Query: 22  LLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKE-LIHNTLGKLADAYGPIFML 81
           L+F   ALF         + P P GAWP+IGHL LL+GKE L++ TLGK+AD YGP   L
Sbjct: 13  LVFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSL 72

Query: 82  RLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRK 141
           RLG+++T +VS +EVAK+CFT NDK  ASRP  AAAK +GY+ A+FG +PYS+ WR +RK
Sbjct: 73  RLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRK 132

Query: 142 IATLELLANHRLEKLQHIPRLEVQSSIDKLYELC---RSNKKALVEMEMWFGDITLNTIF 201
           IATLELL+N RL+ L+H+   E+   +  LY L      ++  +V+++ W  D++LN + 
Sbjct: 133 IATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLNMMV 192

Query: 202 RMVIGKRF--------STAFEGK--------------AFLPSDLFPFLRWFDFGGYKKAM 261
           RMV GKR+          A E +               F  SD FP L WFDF G++K M
Sbjct: 193 RMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEM 252

Query: 262 KKTAKIMDEMLDKWLIEHRERR----------------------------NSDDVVTTRV 321
           K+T + +D +L++W+  HR++R                              D + + + 
Sbjct: 253 KQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITSIKS 312

Query: 322 GC------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESDMKNLIYLQ 381
            C      G  T+ + + WA+SLL++N++ LKK Q E+D  VGRDR V +SD++NL+Y+Q
Sbjct: 313 TCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQ 372

Query: 382 AVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPNVWESPTEFRP 441
           A++KETLRLYPA  L    E IEDCTVAGY++  GTR+ VN++K+QRDP V+  P EFRP
Sbjct: 373 AIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRP 432

Query: 442 ERFLTNE-KSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEIRRPSQDP 459
           ERF+T E K FDV+GQ  +L+PFG+GRR CPG S A+QV++L LAR L  F+++     P
Sbjct: 433 ERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMP 492

[Close]

BLAST of Lsi04G016160 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 380.6 bits (976), Expect = 1.9e-105
Identity = 216/499 (43.29%), Postives = 294/499 (58.92%), Query Frame = 0


Query: 22  LLFFLYALFISFGVRCKRRPPQPGGAWPMIGHLPLLTGKE-LIHNTLGKLADAYGPIFML 81
           L+F   ALF         + P P GAWP+IGHL LL GKE L++ TLGK+AD YGP   L
Sbjct: 13  LVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSL 72

Query: 82  RLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRK 141
           RLG+++T + S +EVAK+CFT NDK  AS    AAAK +GY +           W  +RK
Sbjct: 73  RLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGYVF-----------WLEMRK 132

Query: 142 IATLELLANHRLEKLQHIPRLEVQSSIDKLYELC---RSNKKALVEMEMWFGDITLNTIF 201
           IA +ELL+N RL+ L ++   E+   +  LY L      ++  +V+++ W  D+  N I 
Sbjct: 133 IAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIANMIM 192

Query: 202 RMVIGKRF-------STAFEGKA----------------FLPSDLFPFLRWFDFGGYKKA 261
           RMV GKR+       S+    +A                F  SD FP L W D  G++K 
Sbjct: 193 RMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKE 252

Query: 262 MKKTAKIMDEMLDKWLIEHRERR-------NSDDVVTTRVGC------------------ 321
           MK+T + +D +L++W+  HR++R       N  D V   +                    
Sbjct: 253 MKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHLQYDANTCIK 312

Query: 322 ---------GFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESDMKNLIYL 381
                    G  T+ + + WA+SLL++N++ LKKVQ E+D  VGRDR V +SD+KNL+YL
Sbjct: 313 TTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYL 372

Query: 382 QAVVKETLRLYPAAQLSVPHECIEDCTVAGYHIPAGTRLWVNLYKLQRDPNVWESPTEFR 441
           QA++KETLRLYPAA L    E +EDCTVAGY++P GTRL VN++K+QRDP V+  P EFR
Sbjct: 373 QAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFR 432

Query: 442 PERFLTNE-KSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEIRRPSQD 459
           PERF+T E K FDV+GQ  +L+PFG+GRR CPG S A+Q+++L LAR LH FE++     
Sbjct: 433 PERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDR 492

[Close]

BLAST of Lsi04G016160 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 312.4 bits (799), Expect = 6.3e-85
Identity = 178/470 (37.87%), Postives = 258/470 (54.89%), Query Frame = 0


Query: 42  PQPGGAWPMIGHLPLLTGKELIHNTLGKLADAYGPIFMLRLGTNKTLIVSGWEVAKECFT 101
           P+P GA P+ GHL LL GK+L+   L  ++  +GPIF L+LG  + ++ S  +  K+CFT
Sbjct: 40  PEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFT 99

Query: 102 TNDKKFASRPKFAAAKLLGYNYAMFGTSPYSSHWRHVRKIATLELLANHRLEKLQHIPRL 161
           TND   A+RP  A  + +GYN A    +PY  +WR +RKI T+ L +NH +E L HI   
Sbjct: 100 TNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSS 159

Query: 162 EVQSSIDKLYELCRSNKKALVEMEMWFGDITLNTIFRMVIGKRFSTA------------- 221
           EV + I  LY+   +   ++V+++M F  +T N I R ++GKR                 
Sbjct: 160 EVNTLIKHLYK--GNGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEAL 219

Query: 222 --FEGKAFLP--SDLFPFLRWFDFGGYKKAMKKTAKIMDEMLDKWLIEHRERRN------ 281
              E  A +P   D+ P+L W DF      MK+  K +D +  KWL EH ++R+      
Sbjct: 220 KHCEYLAVIPMIGDVIPWLGWLDF-AKNSQMKRLFKELDSVNTKWLHEHLKKRSRNEKDQ 279

Query: 282 -------------SDDVVTTRVG-------------CGFHTTTAEMVWALSLLVSNEEAL 341
                         D V++  V               G  +T+  + WA+SLL++N  AL
Sbjct: 280 ERTIMDLLLDILPEDIVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAAL 339

Query: 342 KKVQLELDEQVGRDREVRESDMKNLIYLQAVVKETLRLYPAAQLSVPHECIEDCTVAGYH 401
           +  Q E+D  VG+ R + ESD++NL YLQA+VKET RLYP A L+   E  EDC V GY 
Sbjct: 340 EAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYR 399

Query: 402 IPAGTRLWVNLYKLQRDPNVWESPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPG 461
           +  GTRL VN++KL RDP +W  P  F+PERF+  E     +    + IPFG+GRR CPG
Sbjct: 400 VEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFM--EDKSQCEKSNFEYIPFGSGRRSCPG 459

Query: 462 ISFAIQVMNLSLARLLHEFEIRRPSQDPLNMEESVALNITKKIPLETDVV 463
           ++  ++V++  LARLL  FE+ + S +PL+M E   L + K  P+E  V+
Sbjct: 460 VNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVM 504

[Close]

BLAST of Lsi04G016160 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 310.8 bits (795), Expect = 1.8e-84
Identity = 187/508 (36.81%), Postives = 269/508 (52.95%), Query Frame = 0


Query: 22  LLFFLYALFI---SFGVRCKRRP-------PQPGGAWPMIGHLPLLTGKELIHNTLGKLA 81
           +LF L ALFI      ++ + RP       P   GAWP++GHL L       H T G +A
Sbjct: 5   MLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMA 64

Query: 82  DAYGPIFMLRLGTNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAKLLGYNYAMFGTSPY 141
           D YGP+FM +LG+ K +I++  EVAKE +T +D K   RP+  A+KLLGYN +    SPY
Sbjct: 65  DVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHD-KLLERPELTASKLLGYNDSFLTFSPY 124

Query: 142 SSHWRHVRKIATLELLANHRLEKLQHIPRLEVQSSIDKLYELCRSNKK----ALVEMEMW 201
             +WR +RKIA  EL +   ++        E   +   LY       K     LV+M+  
Sbjct: 125 GLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQE 184

Query: 202 FGDITLNTIFRMVIGKRF----------STAFEGK----------AFLPSDLFPFLRWFD 261
           F D+T N    MV GKR+               GK           FL SD+ P L + D
Sbjct: 185 FIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLD 244

Query: 262 FGGYKKAMKKTAKIMDEMLDKWLIEHRERRNSD--------DVVTTRVG----------- 321
           +   K+ MK+TAK +D++ + W+ EH+ +R+          D++   +G           
Sbjct: 245 W-KTKRGMKRTAKGLDKVAEGWIEEHKNKRSDHGRSENDYLDILIKILGQDKIPGLSDTH 304

Query: 322 ------------CGFHTTTAEMVWALSLLVSNEEALKKVQLELDEQVGRDREVRESDMKN 381
                        G  T    +VWA+SLL++N   L+K Q ELD ++G++R V E D+K+
Sbjct: 305 TKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKD 364

Query: 382 LIYLQAVVKETLRLYPAAQLSVPHECIEDCTVA--GYHIPAGTRLWVNLYKLQRDPNVWE 441
           L+YLQA+VKET RLYP   L      +ED  +A    H+PAGT+L V+ +K+ RDPNVW 
Sbjct: 365 LVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWS 424

Query: 442 SPTEFRPERFLTNEKSFDVKGQTPQLIPFGAGRRICPGISFAIQVMNLSLARLLHEFEIR 463
           +P +F PERFLT+ +  DV GQ+ +  PFG GRR CP I   +++++  L R LH F++ 
Sbjct: 425 NPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLA 484

The following BLAST results are available for this feature:

BLAST of Lsi04G016160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49859	1.5e-118	44.34	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
Q9SZ46	1.9e-118	45.69	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
O49394	4.4e-115	44.78	Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=...	[more]
O81972	2.2e-114	44.55	Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1	[more]
A0A0N7F297	7.0e-113	45.26	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]

BLAST of Lsi04G016160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BI41	5.8e-211	73.63	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CMD1	5.8e-211	73.63	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502571 PE=3 SV=1	[more]
A0A6J1FQP2	4.0e-196	68.84	cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1FPF7	1.7e-194	67.72	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446067 PE=3...	[more]
A0A6J1FIL1	8.4e-194	67.85	cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Lsi04G016160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898814.1	9.4e-216	75.94	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_038897312.1	1.2e-215	76.23	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_004147802.1	2.6e-213	73.58	cytochrome P450 CYP82D47 [Cucumis sativus] >KAE8651314.1 hypothetical protein Cs...	[more]
XP_008464764.1	1.2e-210	73.63	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032715.1 cytochrome...	[more]
XP_022940480.1	8.3e-196	68.84	cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Lsi04G016160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	1.4e-119	45.69	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	3.1e-116	44.78	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT4G31950.1	1.9e-105	43.29	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT3G25180.1	6.3e-85	37.87	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT2G25160.1	1.8e-84	36.81	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 410..421 score: 31.04 coord: 269..286 score: 30.52 coord: 322..333 score: 50.13 coord: 401..410 score: 60.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 265..452 e-value: 7.7E-59 score: 199.6 coord: 42..259 e-value: 3.8E-21 score: 75.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 191..209 score: 25.77 coord: 321..339 score: 35.47 coord: 71..90 score: 30.29 coord: 95..116 score: 25.15 coord: 362..386 score: 41.74 coord: 400..410 score: 57.78 coord: 410..433 score: 31.15
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 35..264 e-value: 9.8E-41 score: 141.7
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 265..463 e-value: 2.4E-66 score: 226.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 41..458
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 38..458
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 38..458
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 403..412

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G016160.1	Lsi04G016160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen