Lsi04G016040 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G016040 vs. ExPASy Swiss-Prot
Match: Q8LGM7 (Molybdenum cofactor sulfurase OS=Solanum lycopersicum OX=4081 GN=FLACCA PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.1e-16
Identity = 77/325 (23.69%), Postives = 152/325 (46.77%), Query Frame = 0

Query: 145 KFLTMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTL- 204
           +F + Y    + + ID++R+ E++ L+++   V LD+ G  L+S  Q +  ++    TL 
Sbjct: 13  EFGSYYGYANSPKNIDEIRATEFKRLNDT---VYLDHAGATLYSESQMEAVFKDLNSTLY 72

Query: 205 ----SEITANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLS 264
               S+ T +L+   + G A         + +++ + N S  EY  +FT    +A KL+ 
Sbjct: 73  GNPHSQSTCSLATEDIVGKA---------RQQVLSFFNASPREYSCIFTSGATAALKLVG 132

Query: 265 ESYPFHTNKKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFK----------WPTLRLCS 324
           E++P+ +N   +   ++ + SV  + + A  +GA  ++   +             L+L  
Sbjct: 133 ETFPWSSNSSFMYSMENHN-SVLGIREYALSKGAAAFAVDIEDTHVGESESPQSNLKLTQ 192

Query: 325 RELRKQITNKRKRKKDSVAG----LFVFPVQSRVTGAKYSYQWMALAQQNN--------- 384
             ++++  N+    K+ + G    LF FP +   +G K+    + + ++ +         
Sbjct: 193 HHIQRR--NEGGVLKEGMTGNTYNLFAFPSECNFSGRKFDPNLIKIIKEGSERILESSQY 252

Query: 385 ----WHVLLDAGSLGPKDMDSLGLSLFKPDFIITSFYRVFGSDPTGFGCLLIKKSVIGSL 436
               W VL+DA      +  +  LS+FK DF++ SFY++FG  PTG G L+++K     +
Sbjct: 253 SRGCWLVLIDAAKGCATNPPN--LSMFKADFVVFSFYKLFGY-PTGLGALIVRKDAAKLM 312

BLAST of Lsi04G016040 vs. ExPASy Swiss-Prot
Match: Q9C5X8 (Molybdenum cofactor sulfurase OS=Arabidopsis thaliana OX=3702 GN=ABA3 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.6e-15
Identity = 81/320 (25.31%), Postives = 143/320 (44.69%), Query Frame = 0

Query: 141 DAFTKFLTMYPKFQTSEK-IDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESS 200
           +AF K    Y  +    K I ++R  E++ L +    V LD+ G  L+S +Q +  ++  
Sbjct: 2   EAFLKEFGDYYGYPDGPKNIQEIRDTEFKRLDKGV--VYLDHAGSTLYSELQMEYIFKD- 61

Query: 201 AFTLSEITANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLS 260
            FT S +  N  + +    A    I  D + ++++Y N S  +Y  +FT    +A KL+ 
Sbjct: 62  -FT-SNVFGNPHSQSDISSATSDLIA-DARHQVLEYFNASPEDYSCLFTSGATAALKLVG 121

Query: 261 ESYPFHTNKKLL-TMFDHES----------QSVSWMAQSAKERGAKVYSAWFKWPTLRLC 320
           E++P+  +   L TM +H S          Q  S  A   +E   +        P++++ 
Sbjct: 122 ETFPWTQDSNFLYTMENHNSVLGIREYALAQGASACAVDIEEAANQPGQLTNSGPSIKVK 181

Query: 321 SRELRKQITNK--RKRKKDSVAGLFVFPVQSRVTGAKYSYQWMALAQQN----------- 380
            R ++ + T+K  ++  + +   LF FP +   +G +++   + L ++N           
Sbjct: 182 HRAVQMRNTSKLQKEESRGNAYNLFAFPSECNFSGLRFNLDLVKLMKENTETVLQGSPFS 241

Query: 381 ---NWHVLLDAG---SLGPKDMDSLGLSLFKPDFIITSFYRVFGSDPTGFGCLLIKKSVI 430
               W VL+DA    +  P D     LS +  DF++ SFY++FG  PTG G LL++    
Sbjct: 242 KSKRWMVLIDAAKGCATLPPD-----LSEYPADFVVLSFYKLFGY-PTGLGALLVRNDAA 301

BLAST of Lsi04G016040 vs. ExPASy Swiss-Prot
Match: Q16P90 (Molybdenum cofactor sulfurase 3 OS=Aedes aegypti OX=7159 GN=mal3 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 5.8e-13
Identity = 72/271 (26.57%), Postives = 110/271 (40.59%), Query Frame = 0

Query: 165 EEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTL------SEITANLSNHALYGG 224
           +E+  L E   K  LD+ G  L++  Q +   E  A  L      S  T +L +   Y  
Sbjct: 21  KEFSRLKE---KCYLDHAGTTLYADSQIRSVCEGLAQNLYCNPHTSRTTEDLLDQVRY-- 80

Query: 225 AEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTNKKLLTMFDHES 284
                       R++ + N   +EY L+FT    ++ KLL+ESY F      + + D  +
Sbjct: 81  ------------RVLRHFNTRSSEYSLIFTSGTTASLKLLAESYEFAPEGAFVYLKDSHT 140

Query: 285 QSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSVAGLFVFPVQSR 344
             +        ER   +Y             RE   +  +  +R     + L VFP Q  
Sbjct: 141 SVLGMREIVGTER---IYPV----------EREQLLKELDSSERSDSEHSSLIVFPAQCN 200

Query: 345 VTGAKYSYQWMALAQQN--------NWHVLLDAGSLGPKDMDSLGLSLFKPDFIITSFYR 404
             G KY  + +   Q+N         + V LDA S        L LS ++PDF+  SFY+
Sbjct: 201 FNGVKYPLELVRKIQRNGISGYGKERFRVCLDAASF--VSTSFLDLSKYQPDFVCLSFYK 258

Query: 405 VFGSDPTGFGCLLIKKSVIGSLQNQSGRTGT 422
           +FG  PTG G LL+  +    L+ +    GT
Sbjct: 261 IFGY-PTGLGALLVHHTAADQLRKKYYGGGT 258

BLAST of Lsi04G016040 vs. ExPASy Swiss-Prot
Match: Q16GH0 (Molybdenum cofactor sulfurase 1 OS=Aedes aegypti OX=7159 GN=mal1 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 4.9e-12
Identity = 70/270 (25.93%), Postives = 110/270 (40.74%), Query Frame = 0

Query: 166 EYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTL------SEITANLSNHALYGGA 225
           E+  L E   K  LD+ G  L++  Q +   E  A  L      S  T +L +   Y   
Sbjct: 22  EFSRLKE---KCYLDHAGTTLYADSQIRSVCEGLAQNLYCNPHTSRTTEDLLDQVRY--- 81

Query: 226 EKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTNKKLLTMFDHESQ 285
                      R++ + N   +EY L+FT    ++ KLL+ES+ F      + + D  + 
Sbjct: 82  -----------RVLRHFNTRSSEYSLIFTSGTTASLKLLAESFEFAPEGAFVYLKDSHTS 141

Query: 286 SVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSVAGLFVFPVQSRV 345
            +        ER   +Y             RE   +  +  +R  +  + L VFP Q   
Sbjct: 142 VLGMREIVGTER---IYPV----------EREQLLKELDSSERSDNEHSSLIVFPAQCNF 201

Query: 346 TGAKYSYQWMALAQQN--------NWHVLLDAGSLGPKDMDSLGLSLFKPDFIITSFYRV 405
            G KY  + +   Q++         + V LDA S        L LS ++PDF+  SFY++
Sbjct: 202 NGVKYPLELVRKIQRDGISGYGKERFRVCLDAASF--VSTSFLDLSKYQPDFVCLSFYKI 258

Query: 406 FGSDPTGFGCLLIKKSVIGSLQNQSGRTGT 422
           FG  PTG G LL+  +    L+ +    GT
Sbjct: 262 FGY-PTGLGALLVHHTAADQLRKKYYGGGT 258

BLAST of Lsi04G016040 vs. ExPASy Swiss-Prot
Match: A2VD33 (Molybdenum cofactor sulfurase OS=Danio rerio OX=7955 GN=mocos PE=2 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 4.9e-12
Identity = 66/256 (25.78%), Postives = 111/256 (43.36%), Query Frame = 0

Query: 179 LDYCGFGLFSHIQTQQFWESSAFTLSEITANLSNHALYGGAEKGTIEHDIKTRIMDYLNI 238
           LD+ G  LF     + F +  +     +  N  +H         T+E  ++ +I+ + N 
Sbjct: 48  LDHAGTTLFPESLIKGFHDDIS---RNVYGNPHSHNSSSRLTHDTVE-SVRYKILAHFNT 107

Query: 239 SENEYGLVFTVSRGSAFKLLSESYPFHTNKKLLTMFDHESQSVSWMAQSAKERGAKVYSA 298
           S  +Y ++FT    +A KL+++++P+    K ++  +  SQ            G +  +A
Sbjct: 108 SPEDYSVIFTSGCTAALKLVADTFPW----KPMSNKEPGSQFCYLTDNHTSVVGIRGATA 167

Query: 299 WFKWPTLRLCSRELRKQITNKRK---RKKDSVAGLFVFPVQSRVTGAKYSYQWM------ 358
                T+ +  RE+  +  NK +    ++ S   LF +P QS  +G KYS  ++      
Sbjct: 168 LQGVGTISVSPREVETRARNKTQTNGEEECSTPHLFCYPAQSNFSGRKYSLSYVKGIQSQ 227

Query: 359 ----ALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITSFYRVFGSDPTGFGCLLIK 418
               A      W VLLDA          L LS +  DF+  SFY++FG  PTG G LL++
Sbjct: 228 QLYPACEHHGQWFVLLDAACF--VSCSPLDLSQYPADFVPISFYKMFGF-PTGLGALLVR 287

Query: 419 KSVIGSLQNQSGRTGT 422
                 L+      GT
Sbjct: 288 NEAAEVLRKTYFGGGT 292

BLAST of Lsi04G016040 vs. ExPASy TrEMBL
Match: A0A5A7UPY9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1920G00320 PE=4 SV=1)

HSP 1 Score: 1773.8 bits (4593), Expect = 0.0e+00
Identity = 903/951 (94.95%), Postives = 916/951 (96.32%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           K+RDIDCESPNQDRNVRRSRS ARLHAQKEFLRATALAADRTY  ED IPNLFDAFTKFL
Sbjct: 61  KTRDIDCESPNQDRNVRRSRSFARLHAQKEFLRATALAADRTYCREDSIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAF+LSEIT
Sbjct: 121 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFSLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN
Sbjct: 181 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+QSGRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQSGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD IN  EDSETEKH ESRMPAFSGVFT NQVRDVFETEME DNNSSDRDGASTIFEE
Sbjct: 421 IEDDVIN--EDSETEKHPESRMPAFSGVFTPNQVRDVFETEMEHDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR 567
           AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNS HLIKQKTWSPLPPSWFSGKR
Sbjct: 481 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSDHLIKQKTWSPLPPSWFSGKR 540

Query: 568 NTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEEQS 627
           N RKRSPKPASRLLKSPMC +DKR N+RH  DSVLSFDAA+LSMSQDF CV+GIPEEEQS
Sbjct: 541 NNRKRSPKPASRLLKSPMCSNDKRANARHRNDSVLSFDAALLSMSQDFSCVQGIPEEEQS 600

Query: 628 GEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQDLKRSNST 687
           GEQDSCCGNVGSLRDSH VSEIQEDSETGEES RLS ASNG  PAN TSEF DLKRSNST
Sbjct: 601 GEQDSCCGNVGSLRDSHVVSEIQEDSETGEESARLSFASNGIHPANHTSEFWDLKRSNST 660

Query: 688 TSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFSVEYN 747
           TSGAF DLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMG  VSF VEYN
Sbjct: 661 TSGAFNDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGRRVSFRVEYN 720

Query: 748 EKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGLNKTT 807
           EKESLNEMFELGE S  AFGNEESTSDGEYVDEQEWGRRE EMICRHLDHIDMLGLNKTT
Sbjct: 721 EKESLNEMFELGEASCTAFGNEESTSDGEYVDEQEWGRREPEMICRHLDHIDMLGLNKTT 780

Query: 808 LRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRGLIHP 867
           LR RYLINWLVTSLLQLRLPG+DDVGV LVQLYGPKIKYERGAAIAFN+KESNGRGLIHP
Sbjct: 781 LRQRYLINWLVTSLLQLRLPGQDDVGVQLVQLYGPKIKYERGAAIAFNVKESNGRGLIHP 840

Query: 868 EVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 927
           EVVQKLAENNGI+LGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE
Sbjct: 841 EVVQKLAENNGIALGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 900

Query: 928 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF 979
           VVTASLGFLTNFDDVYKMWAF+AKFLNPSFLENNTLSSVPESSESYHRS+F
Sbjct: 901 VVTASLGFLTNFDDVYKMWAFVAKFLNPSFLENNTLSSVPESSESYHRSMF 948

BLAST of Lsi04G016040 vs. ExPASy TrEMBL
Match: A0A1S3BZ97 (uncharacterized protein LOC103495022 OS=Cucumis melo OX=3656 GN=LOC103495022 PE=4 SV=1)

HSP 1 Score: 1773.8 bits (4593), Expect = 0.0e+00
Identity = 903/951 (94.95%), Postives = 916/951 (96.32%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           K+RDIDCESPNQDRNVRRSRS ARLHAQKEFLRATALAADRTY  ED IPNLFDAFTKFL
Sbjct: 61  KTRDIDCESPNQDRNVRRSRSFARLHAQKEFLRATALAADRTYCREDSIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAF+LSEIT
Sbjct: 121 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFSLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN
Sbjct: 181 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+QSGRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQSGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD IN  EDSETEKH ESRMPAFSGVFT NQVRDVFETEME DNNSSDRDGASTIFEE
Sbjct: 421 IEDDVIN--EDSETEKHPESRMPAFSGVFTPNQVRDVFETEMEHDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR 567
           AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNS HLIKQKTWSPLPPSWFSGKR
Sbjct: 481 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSDHLIKQKTWSPLPPSWFSGKR 540

Query: 568 NTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEEQS 627
           N RKRSPKPASRLLKSPMC +DKR N+RH  DSVLSFDAA+LSMSQDF CV+GIPEEEQS
Sbjct: 541 NNRKRSPKPASRLLKSPMCSNDKRANARHRNDSVLSFDAALLSMSQDFSCVQGIPEEEQS 600

Query: 628 GEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQDLKRSNST 687
           GEQDSCCGNVGSLRDSH VSEIQEDSETGEES RLS ASNG  PAN TSEF DLKRSNST
Sbjct: 601 GEQDSCCGNVGSLRDSHVVSEIQEDSETGEESARLSFASNGIHPANHTSEFWDLKRSNST 660

Query: 688 TSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFSVEYN 747
           TSGAF DLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMG  VSF VEYN
Sbjct: 661 TSGAFNDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGRRVSFRVEYN 720

Query: 748 EKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGLNKTT 807
           EKESLNEMFELGE S  AFGNEESTSDGEYVDEQEWGRRE EMICRHLDHIDMLGLNKTT
Sbjct: 721 EKESLNEMFELGEASCTAFGNEESTSDGEYVDEQEWGRREPEMICRHLDHIDMLGLNKTT 780

Query: 808 LRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRGLIHP 867
           LR RYLINWLVTSLLQLRLPG+DDVGV LVQLYGPKIKYERGAAIAFN+KESNGRGLIHP
Sbjct: 781 LRQRYLINWLVTSLLQLRLPGQDDVGVQLVQLYGPKIKYERGAAIAFNVKESNGRGLIHP 840

Query: 868 EVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 927
           EVVQKLAENNGI+LGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE
Sbjct: 841 EVVQKLAENNGIALGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 900

Query: 928 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF 979
           VVTASLGFLTNFDDVYKMWAF+AKFLNPSFLENNTLSSVPESSESYHRS+F
Sbjct: 901 VVTASLGFLTNFDDVYKMWAFVAKFLNPSFLENNTLSSVPESSESYHRSMF 948

BLAST of Lsi04G016040 vs. ExPASy TrEMBL
Match: A0A0A0LIQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852500 PE=4 SV=1)

HSP 1 Score: 1770.4 bits (4584), Expect = 0.0e+00
Identity = 900/951 (94.64%), Postives = 916/951 (96.32%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKKTR+RHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKTRKRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           K+RDIDC+SP+QDRNVRRSRS ARLHAQKEFLRATALAADRTY TED IPNLFDAFTKFL
Sbjct: 61  KTRDIDCDSPDQDRNVRRSRSFARLHAQKEFLRATALAADRTYCTEDLIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT
Sbjct: 121 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN
Sbjct: 181 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+QSGRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQSGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           I+DD IN  EDSETEKH ESRMPAFSGVFT NQVRDVFETEME DNNSSDRDGASTIFEE
Sbjct: 421 IDDDVIN--EDSETEKHLESRMPAFSGVFTPNQVRDVFETEMEHDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR 567
           AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNS HLIKQKTWSPLPPSWFSGKR
Sbjct: 481 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSDHLIKQKTWSPLPPSWFSGKR 540

Query: 568 NTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEEQS 627
           N R+RSPKPASRLLKSPMCGDDKR N+RH  DSVLSFDAAVLSMSQDF CV+GIPEE+QS
Sbjct: 541 NNRQRSPKPASRLLKSPMCGDDKRANARHRNDSVLSFDAAVLSMSQDFSCVEGIPEEDQS 600

Query: 628 GEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQDLKRSNST 687
           GEQDSCCGNVGSLRDSH VSEIQEDSETGEES RLS ASNG  P N TSEF+DLKRSNST
Sbjct: 601 GEQDSCCGNVGSLRDSHVVSEIQEDSETGEESARLSFASNGIHPVNHTSEFRDLKRSNST 660

Query: 688 TSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFSVEYN 747
           TSGAF DLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMG  VSF VEYN
Sbjct: 661 TSGAFNDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGRRVSFRVEYN 720

Query: 748 EKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGLNKTT 807
           EKESLNEMFELGETS  AFGNEESTSDGEYVDEQEWGRRE EMICRHLDHIDMLGLNKTT
Sbjct: 721 EKESLNEMFELGETSCTAFGNEESTSDGEYVDEQEWGRREPEMICRHLDHIDMLGLNKTT 780

Query: 808 LRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRGLIHP 867
           LR RYLINWLVTSLLQLRLPG+DDVGVHLVQLYGPKIKYERGAAIAFN+KESNGRGLIHP
Sbjct: 781 LRQRYLINWLVTSLLQLRLPGQDDVGVHLVQLYGPKIKYERGAAIAFNVKESNGRGLIHP 840

Query: 868 EVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 927
           EVVQKLAENNGI+LGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE
Sbjct: 841 EVVQKLAENNGIALGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 900

Query: 928 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF 979
           VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLS VPES ESY  S+F
Sbjct: 901 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSPVPESLESYRGSMF 948

BLAST of Lsi04G016040 vs. ExPASy TrEMBL
Match: A0A6J1FL08 (uncharacterized protein LOC111446707 OS=Cucurbita moschata OX=3662 GN=LOC111446707 PE=4 SV=1)

HSP 1 Score: 1706.8 bits (4419), Expect = 0.0e+00
Identity = 870/949 (91.68%), Postives = 910/949 (95.89%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKK+RRRHGSG+TERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKSRRRHGSGLTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           KSRDIDC+SPN D NVRRSRSLARLHAQKEFLRATALAADRTYSTED IPNLFDAFTKFL
Sbjct: 61  KSRDIDCDSPNHDGNVRRSRSLARLHAQKEFLRATALAADRTYSTEDLIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQ+SE+IDQLR+E+YEHLSESFSKVCLDYCGFGLFS+IQTQQFWESSAFTLSEIT
Sbjct: 121 TMYPKFQSSEQIDQLRTEDYEHLSESFSKVCLDYCGFGLFSYIQTQQFWESSAFTLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANL+NHALYGGAEKGTIEHDIKTRIM+YLNISENEYGLVFTVSRGSAFKLL+ESYPFHTN
Sbjct: 181 ANLNNHALYGGAEKGTIEHDIKTRIMNYLNISENEYGLVFTVSRGSAFKLLAESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQ+AKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQNAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           +GLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 SGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+Q GRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQCGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD INGQEDSETE HQESRMPAFSGVFT+NQVRDVFETE+EQDNNSSDRDGASTIFEE
Sbjct: 421 IEDDAINGQEDSETETHQESRMPAFSGVFTTNQVRDVFETEIEQDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTW-SPLPPSWFSGK 567
            ESIS+GEVMKSPIFSEDESSDNSYWIDLG SPFGSDNSGHLIKQKTW SPLPPSWFSGK
Sbjct: 481 VESISVGEVMKSPIFSEDESSDNSYWIDLGHSPFGSDNSGHLIKQKTWSSPLPPSWFSGK 540

Query: 568 RNTRKRSPKPASRLLKSPMC-GDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEE 627
           RN+R+ SPKPASRLL+SP+C GDDKR N RH +DSVLSFDAAVLS+SQD   V+GIPEEE
Sbjct: 541 RNSRQLSPKPASRLLRSPICGGDDKRANPRHRDDSVLSFDAAVLSVSQDLCRVEGIPEEE 600

Query: 628 QSGEQDSCCGNVGSLRDSHAVSEIQEDSETGEES--GRLSVASNGTRPANQTSEFQDLKR 687
           QSGEQDSCCGNVGSL+DSHAVSEIQEDSETGEES   RLS ASNG R ANQT E +DLK 
Sbjct: 601 QSGEQDSCCGNVGSLKDSHAVSEIQEDSETGEESIPNRLSFASNGNRSANQTFEIRDLKL 660

Query: 688 SNSTTSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFS 747
           SNST +GA KDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL +G+RA+SMG  VSFS
Sbjct: 661 SNSTAAGALKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL-DGERALSMGRRVSFS 720

Query: 748 VEYNEKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGL 807
           VEYNEKESLNEMFELGE SNAAF NEES SDGEYVDEQEWGRRE EMICRHLDHIDMLGL
Sbjct: 721 VEYNEKESLNEMFELGEASNAAFDNEESMSDGEYVDEQEWGRREPEMICRHLDHIDMLGL 780

Query: 808 NKTTLRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRG 867
           N+TTLRLRYLINWLVTSLLQLRLPGRDDVG HLVQLYGPKIKYERGAA+AFN+KESNGRG
Sbjct: 781 NRTTLRLRYLINWLVTSLLQLRLPGRDDVGTHLVQLYGPKIKYERGAAVAFNVKESNGRG 840

Query: 868 LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLF 927
           LIHPEVVQ+LAENNGISLGVGILSHVRAVDVPKQNSGQYDL+DMALCKPM NGHNRKKLF
Sbjct: 841 LIHPEVVQRLAENNGISLGVGILSHVRAVDVPKQNSGQYDLKDMALCKPMANGHNRKKLF 900

Query: 928 FRVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSES 973
           FRVEVVTASLGFLTNF+DVYKMWAF+AKFLNPSFLEN+TLSS PE+SES
Sbjct: 901 FRVEVVTASLGFLTNFEDVYKMWAFVAKFLNPSFLENSTLSSGPETSES 947

BLAST of Lsi04G016040 vs. ExPASy TrEMBL
Match: A0A6J1IYP0 (uncharacterized protein LOC111481100 OS=Cucurbita maxima OX=3661 GN=LOC111481100 PE=4 SV=1)

HSP 1 Score: 1706.0 bits (4417), Expect = 0.0e+00
Identity = 870/949 (91.68%), Postives = 909/949 (95.79%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKK+RRRHGSG+TERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKSRRRHGSGLTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           KSRDIDC+SPN D NVRRSRSLARLHAQKEFLRATALAADRTYSTED IPNLFDAFTKFL
Sbjct: 61  KSRDIDCDSPNHDGNVRRSRSLARLHAQKEFLRATALAADRTYSTEDLIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQ+SE+IDQLR+E+YEHLSESFSKVCLDYCGFGLFS+IQTQQFWESSAFTLSEIT
Sbjct: 121 TMYPKFQSSEQIDQLRTEDYEHLSESFSKVCLDYCGFGLFSYIQTQQFWESSAFTLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANL+NHALYGGAEKGTIEHDIKTRIM+YLNISENEYGLVFTVSRGSAFKLL+ESYPFHTN
Sbjct: 181 ANLNNHALYGGAEKGTIEHDIKTRIMNYLNISENEYGLVFTVSRGSAFKLLAESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQ+AKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQNAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           +GLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 SGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+Q GRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQCGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD INGQEDSETE HQESRMPAFSGVFT+NQVRDVFETE+EQDNNSSDRDGASTIFEE
Sbjct: 421 IEDDAINGQEDSETETHQESRMPAFSGVFTTNQVRDVFETEIEQDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTW-SPLPPSWFSGK 567
            ESIS+GEVMKSPIFSEDESSDNSYWIDLG SPFGSDNSGHLIKQKTW SPLPPSWFSGK
Sbjct: 481 VESISVGEVMKSPIFSEDESSDNSYWIDLGHSPFGSDNSGHLIKQKTWSSPLPPSWFSGK 540

Query: 568 RNTRKRSPKPASRLLKSPMC-GDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEE 627
           RN+R+ SPKPASRLL+SP+C GDDKR N RH +DSVLSFDAAVLS+SQD   V+GIPEEE
Sbjct: 541 RNSRQLSPKPASRLLRSPICGGDDKRANPRHRDDSVLSFDAAVLSVSQDLCRVEGIPEEE 600

Query: 628 QSGEQDSCCGNVGSLRDSHAVSEIQEDSETGEE--SGRLSVASNGTRPANQTSEFQDLKR 687
           QSGEQDSCCGNVGSL+DSHAVSEIQEDSETGEE  S RLS ASNG R ANQT E QDLK 
Sbjct: 601 QSGEQDSCCGNVGSLKDSHAVSEIQEDSETGEESISNRLSFASNGNRSANQTFEIQDLKL 660

Query: 688 SNSTTSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFS 747
           SNST +GA KDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL +G+RA+SMG  VSFS
Sbjct: 661 SNSTAAGALKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL-DGERALSMGRRVSFS 720

Query: 748 VEYNEKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGL 807
           VEYNEKESLNEMFELGE SNAAF NEES SDGEYVDEQEWGRRE EMICRHLDHIDMLGL
Sbjct: 721 VEYNEKESLNEMFELGEASNAAFDNEESMSDGEYVDEQEWGRREPEMICRHLDHIDMLGL 780

Query: 808 NKTTLRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRG 867
           N+TTLRLRYLINWLVTSLLQLRLPGRDDVG HLVQLYGPKIKYERGAA+AFN+KESNGRG
Sbjct: 781 NRTTLRLRYLINWLVTSLLQLRLPGRDDVGTHLVQLYGPKIKYERGAAVAFNVKESNGRG 840

Query: 868 LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLF 927
           LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDL+DMALCKPM NGHNRKKLF
Sbjct: 841 LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLKDMALCKPMANGHNRKKLF 900

Query: 928 FRVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSES 973
           FRVEVVT SLGFLTNF+DVYKMWAF+AKFLNPSFLE++TLSS PE+SES
Sbjct: 901 FRVEVVTVSLGFLTNFEDVYKMWAFVAKFLNPSFLESSTLSSGPETSES 947

BLAST of Lsi04G016040 vs. NCBI nr
Match: XP_038899790.1 (uncharacterized protein LOC120087021 [Benincasa hispida])

HSP 1 Score: 1812.7 bits (4694), Expect = 0.0e+00
Identity = 918/951 (96.53%), Postives = 930/951 (97.79%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           KSRDIDCESPN DRNVRRSRSLARLHAQKEFLRATALAADRTY TED IPNLFDAFTKFL
Sbjct: 61  KSRDIDCESPNYDRNVRRSRSLARLHAQKEFLRATALAADRTYCTEDLIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESS+FTLSEIT
Sbjct: 121 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSSFTLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN
Sbjct: 181 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQSAK+RGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQSAKQRGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD INGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETE+E DNNSSDRDGASTIFEE
Sbjct: 421 IEDDAINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEIEHDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR 567
           AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR
Sbjct: 481 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR 540

Query: 568 NTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEEQS 627
           N R+RSPKPASR LKSPMCGDDKR+NSR +EDSVLSFDAAVLSMSQDFGCVKGIPEEEQS
Sbjct: 541 NNRQRSPKPASRFLKSPMCGDDKRVNSRQHEDSVLSFDAAVLSMSQDFGCVKGIPEEEQS 600

Query: 628 GEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQDLKRSNST 687
           GEQDSCCGNVGSL+DSH VSEIQEDSETGEES RLSVASNG RPAN TSEFQ+LKRSNST
Sbjct: 601 GEQDSCCGNVGSLKDSHVVSEIQEDSETGEESARLSVASNGIRPANDTSEFQELKRSNST 660

Query: 688 TSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFSVEYN 747
           T GAFKDLKE+AIRRETEGEFRLLGRRERSRFSERGF GLEEGDRAISMG  VSFSVEYN
Sbjct: 661 TCGAFKDLKENAIRRETEGEFRLLGRRERSRFSERGFLGLEEGDRAISMGRRVSFSVEYN 720

Query: 748 EKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGLNKTT 807
           EKESLNEMFELGE SNAAFGNEESTSDGEYVDEQEWGRRE EMICRHLDHIDMLGLNKTT
Sbjct: 721 EKESLNEMFELGEASNAAFGNEESTSDGEYVDEQEWGRREPEMICRHLDHIDMLGLNKTT 780

Query: 808 LRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRGLIHP 867
           LR RYLINWLVTSLLQLRLPG+DDVGVHLVQLYGPKIKYERGAAIAFN+KESNGRGLIHP
Sbjct: 781 LRQRYLINWLVTSLLQLRLPGQDDVGVHLVQLYGPKIKYERGAAIAFNVKESNGRGLIHP 840

Query: 868 EVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 927
           EVVQKLAENNGISLGVGILSHVR VDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE
Sbjct: 841 EVVQKLAENNGISLGVGILSHVRVVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 900

Query: 928 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF 979
           VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF
Sbjct: 901 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF 950

BLAST of Lsi04G016040 vs. NCBI nr
Match: XP_008454669.1 (PREDICTED: uncharacterized protein LOC103495022 [Cucumis melo] >KAA0057170.1 uncharacterized protein E6C27_scaffold741G00400 [Cucumis melo var. makuwa] >TYK27153.1 uncharacterized protein E5676_scaffold1920G00320 [Cucumis melo var. makuwa])

HSP 1 Score: 1773.8 bits (4593), Expect = 0.0e+00
Identity = 903/951 (94.95%), Postives = 916/951 (96.32%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           K+RDIDCESPNQDRNVRRSRS ARLHAQKEFLRATALAADRTY  ED IPNLFDAFTKFL
Sbjct: 61  KTRDIDCESPNQDRNVRRSRSFARLHAQKEFLRATALAADRTYCREDSIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAF+LSEIT
Sbjct: 121 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFSLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN
Sbjct: 181 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+QSGRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQSGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD IN  EDSETEKH ESRMPAFSGVFT NQVRDVFETEME DNNSSDRDGASTIFEE
Sbjct: 421 IEDDVIN--EDSETEKHPESRMPAFSGVFTPNQVRDVFETEMEHDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR 567
           AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNS HLIKQKTWSPLPPSWFSGKR
Sbjct: 481 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSDHLIKQKTWSPLPPSWFSGKR 540

Query: 568 NTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEEQS 627
           N RKRSPKPASRLLKSPMC +DKR N+RH  DSVLSFDAA+LSMSQDF CV+GIPEEEQS
Sbjct: 541 NNRKRSPKPASRLLKSPMCSNDKRANARHRNDSVLSFDAALLSMSQDFSCVQGIPEEEQS 600

Query: 628 GEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQDLKRSNST 687
           GEQDSCCGNVGSLRDSH VSEIQEDSETGEES RLS ASNG  PAN TSEF DLKRSNST
Sbjct: 601 GEQDSCCGNVGSLRDSHVVSEIQEDSETGEESARLSFASNGIHPANHTSEFWDLKRSNST 660

Query: 688 TSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFSVEYN 747
           TSGAF DLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMG  VSF VEYN
Sbjct: 661 TSGAFNDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGRRVSFRVEYN 720

Query: 748 EKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGLNKTT 807
           EKESLNEMFELGE S  AFGNEESTSDGEYVDEQEWGRRE EMICRHLDHIDMLGLNKTT
Sbjct: 721 EKESLNEMFELGEASCTAFGNEESTSDGEYVDEQEWGRREPEMICRHLDHIDMLGLNKTT 780

Query: 808 LRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRGLIHP 867
           LR RYLINWLVTSLLQLRLPG+DDVGV LVQLYGPKIKYERGAAIAFN+KESNGRGLIHP
Sbjct: 781 LRQRYLINWLVTSLLQLRLPGQDDVGVQLVQLYGPKIKYERGAAIAFNVKESNGRGLIHP 840

Query: 868 EVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 927
           EVVQKLAENNGI+LGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE
Sbjct: 841 EVVQKLAENNGIALGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 900

Query: 928 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF 979
           VVTASLGFLTNFDDVYKMWAF+AKFLNPSFLENNTLSSVPESSESYHRS+F
Sbjct: 901 VVTASLGFLTNFDDVYKMWAFVAKFLNPSFLENNTLSSVPESSESYHRSMF 948

BLAST of Lsi04G016040 vs. NCBI nr
Match: XP_011652392.1 (uncharacterized protein LOC101215138 [Cucumis sativus] >KGN59901.1 hypothetical protein Csa_002100 [Cucumis sativus])

HSP 1 Score: 1770.4 bits (4584), Expect = 0.0e+00
Identity = 900/951 (94.64%), Postives = 916/951 (96.32%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKKTR+RHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKTRKRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           K+RDIDC+SP+QDRNVRRSRS ARLHAQKEFLRATALAADRTY TED IPNLFDAFTKFL
Sbjct: 61  KTRDIDCDSPDQDRNVRRSRSFARLHAQKEFLRATALAADRTYCTEDLIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT
Sbjct: 121 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN
Sbjct: 181 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+QSGRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQSGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           I+DD IN  EDSETEKH ESRMPAFSGVFT NQVRDVFETEME DNNSSDRDGASTIFEE
Sbjct: 421 IDDDVIN--EDSETEKHLESRMPAFSGVFTPNQVRDVFETEMEHDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGKR 567
           AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNS HLIKQKTWSPLPPSWFSGKR
Sbjct: 481 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSDHLIKQKTWSPLPPSWFSGKR 540

Query: 568 NTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEEQS 627
           N R+RSPKPASRLLKSPMCGDDKR N+RH  DSVLSFDAAVLSMSQDF CV+GIPEE+QS
Sbjct: 541 NNRQRSPKPASRLLKSPMCGDDKRANARHRNDSVLSFDAAVLSMSQDFSCVEGIPEEDQS 600

Query: 628 GEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQDLKRSNST 687
           GEQDSCCGNVGSLRDSH VSEIQEDSETGEES RLS ASNG  P N TSEF+DLKRSNST
Sbjct: 601 GEQDSCCGNVGSLRDSHVVSEIQEDSETGEESARLSFASNGIHPVNHTSEFRDLKRSNST 660

Query: 688 TSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFSVEYN 747
           TSGAF DLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMG  VSF VEYN
Sbjct: 661 TSGAFNDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGRRVSFRVEYN 720

Query: 748 EKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGLNKTT 807
           EKESLNEMFELGETS  AFGNEESTSDGEYVDEQEWGRRE EMICRHLDHIDMLGLNKTT
Sbjct: 721 EKESLNEMFELGETSCTAFGNEESTSDGEYVDEQEWGRREPEMICRHLDHIDMLGLNKTT 780

Query: 808 LRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRGLIHP 867
           LR RYLINWLVTSLLQLRLPG+DDVGVHLVQLYGPKIKYERGAAIAFN+KESNGRGLIHP
Sbjct: 781 LRQRYLINWLVTSLLQLRLPGQDDVGVHLVQLYGPKIKYERGAAIAFNVKESNGRGLIHP 840

Query: 868 EVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 927
           EVVQKLAENNGI+LGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE
Sbjct: 841 EVVQKLAENNGIALGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFFRVE 900

Query: 928 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSESYHRSLF 979
           VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLS VPES ESY  S+F
Sbjct: 901 VVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSPVPESLESYRGSMF 948

BLAST of Lsi04G016040 vs. NCBI nr
Match: KAG6608507.1 (Molybdenum cofactor sulfurase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1707.2 bits (4420), Expect = 0.0e+00
Identity = 870/949 (91.68%), Postives = 910/949 (95.89%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKK+RRRHGSG+TERR+SSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKSRRRHGSGLTERRQSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           KSRDIDC+SPN D NVRRSRSLARLHAQKEFLRATALAADRTYSTED IPNLFDAFTKFL
Sbjct: 61  KSRDIDCDSPNHDGNVRRSRSLARLHAQKEFLRATALAADRTYSTEDLIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQ+SE+IDQLR+E+YEHLSESFSKVCLDYCGFGLFS+IQTQQFWESSAFTLSEIT
Sbjct: 121 TMYPKFQSSEQIDQLRTEDYEHLSESFSKVCLDYCGFGLFSYIQTQQFWESSAFTLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANL+NHALYGGAEKGTIEHDIKTRIM+YLNISENEYGLVFTVSRGSAFKLL+ESYPFHTN
Sbjct: 181 ANLNNHALYGGAEKGTIEHDIKTRIMNYLNISENEYGLVFTVSRGSAFKLLAESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQ+AKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQNAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           +GLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 SGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+Q GRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQCGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD INGQEDSETE HQESRMPAFSGVFT+NQVRDVFETE+EQDNNSSDRDGASTIFEE
Sbjct: 421 IEDDAINGQEDSETETHQESRMPAFSGVFTTNQVRDVFETEIEQDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTW-SPLPPSWFSGK 567
            ESIS+GEVMKSPIFSEDESSDNSYWIDLG SPFGSDNSGHLIKQKTW SPLPPSWFSGK
Sbjct: 481 VESISVGEVMKSPIFSEDESSDNSYWIDLGHSPFGSDNSGHLIKQKTWSSPLPPSWFSGK 540

Query: 568 RNTRKRSPKPASRLLKSPMC-GDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEE 627
           RN+R+ SPKPASRLL+SP+C GDDKR N RH +DSVLSFDAAVLS+SQD   V+GIPEEE
Sbjct: 541 RNSRQLSPKPASRLLRSPICGGDDKRANPRHRDDSVLSFDAAVLSVSQDLCRVEGIPEEE 600

Query: 628 QSGEQDSCCGNVGSLRDSHAVSEIQEDSETGEES--GRLSVASNGTRPANQTSEFQDLKR 687
           QSGEQDSCCGNVGSL+DSHAV EIQEDSETGEES   RLS ASNG R ANQT E QDLK 
Sbjct: 601 QSGEQDSCCGNVGSLKDSHAVGEIQEDSETGEESIPNRLSFASNGNRSANQTFEIQDLKL 660

Query: 688 SNSTTSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFS 747
           SNST +GAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL +G+RA+SMG  VSFS
Sbjct: 661 SNSTAAGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL-DGERALSMGRRVSFS 720

Query: 748 VEYNEKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGL 807
           VEYNEKESLNEMFELGE SNAAF NEES SDGEYVDEQEWGRRE EMIC+HLDHIDMLGL
Sbjct: 721 VEYNEKESLNEMFELGEASNAAFDNEESMSDGEYVDEQEWGRREPEMICQHLDHIDMLGL 780

Query: 808 NKTTLRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRG 867
           N+TTLRLRYLINWLVTSLLQLRLPGRDDVG HLVQLYGPKIKYERGAA+AFN+KESNGRG
Sbjct: 781 NRTTLRLRYLINWLVTSLLQLRLPGRDDVGTHLVQLYGPKIKYERGAAVAFNVKESNGRG 840

Query: 868 LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLF 927
           LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDL+DMALCKPM NGHNRKKLF
Sbjct: 841 LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLKDMALCKPMANGHNRKKLF 900

Query: 928 FRVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSES 973
           FRVEVVTASLGFLTNF+DVYKMWAF+AKFLNPSFLEN+TLSS PE+SES
Sbjct: 901 FRVEVVTASLGFLTNFEDVYKMWAFVAKFLNPSFLENSTLSSGPETSES 947

BLAST of Lsi04G016040 vs. NCBI nr
Match: XP_022941406.1 (uncharacterized protein LOC111446707 [Cucurbita moschata])

HSP 1 Score: 1706.8 bits (4419), Expect = 0.0e+00
Identity = 870/949 (91.68%), Postives = 910/949 (95.89%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSLA 87
           MHLSLWKPLSHCAALIMDKK+RRRHGSG+TERRKSSILRQLQENKLREALEEASEDGSLA
Sbjct: 1   MHLSLWKPLSHCAALIMDKKSRRRHGSGLTERRKSSILRQLQENKLREALEEASEDGSLA 60

Query: 88  KSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFL 147
           KSRDIDC+SPN D NVRRSRSLARLHAQKEFLRATALAADRTYSTED IPNLFDAFTKFL
Sbjct: 61  KSRDIDCDSPNHDGNVRRSRSLARLHAQKEFLRATALAADRTYSTEDLIPNLFDAFTKFL 120

Query: 148 TMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEIT 207
           TMYPKFQ+SE+IDQLR+E+YEHLSESFSKVCLDYCGFGLFS+IQTQQFWESSAFTLSEIT
Sbjct: 121 TMYPKFQSSEQIDQLRTEDYEHLSESFSKVCLDYCGFGLFSYIQTQQFWESSAFTLSEIT 180

Query: 208 ANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTN 267
           ANL+NHALYGGAEKGTIEHDIKTRIM+YLNISENEYGLVFTVSRGSAFKLL+ESYPFHTN
Sbjct: 181 ANLNNHALYGGAEKGTIEHDIKTRIMNYLNISENEYGLVFTVSRGSAFKLLAESYPFHTN 240

Query: 268 KKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 327
           KKLLTMFDHESQSVSWMAQ+AKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV
Sbjct: 241 KKLLTMFDHESQSVSWMAQNAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSV 300

Query: 328 AGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITS 387
           +GLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+PDFIITS
Sbjct: 301 SGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITS 360

Query: 388 FYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLAG 447
           FYRVFGSDPTGFGCLLIKKSVIGSLQ+Q GRTGTGMVRILPIFPQYIGDSIDGL DVLAG
Sbjct: 361 FYRVFGSDPTGFGCLLIKKSVIGSLQSQCGRTGTGMVRILPIFPQYIGDSIDGL-DVLAG 420

Query: 448 IEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFEE 507
           IEDD INGQEDSETE HQESRMPAFSGVFT+NQVRDVFETE+EQDNNSSDRDGASTIFEE
Sbjct: 421 IEDDAINGQEDSETETHQESRMPAFSGVFTTNQVRDVFETEIEQDNNSSDRDGASTIFEE 480

Query: 508 AESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTW-SPLPPSWFSGK 567
            ESIS+GEVMKSPIFSEDESSDNSYWIDLG SPFGSDNSGHLIKQKTW SPLPPSWFSGK
Sbjct: 481 VESISVGEVMKSPIFSEDESSDNSYWIDLGHSPFGSDNSGHLIKQKTWSSPLPPSWFSGK 540

Query: 568 RNTRKRSPKPASRLLKSPMC-GDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEE 627
           RN+R+ SPKPASRLL+SP+C GDDKR N RH +DSVLSFDAAVLS+SQD   V+GIPEEE
Sbjct: 541 RNSRQLSPKPASRLLRSPICGGDDKRANPRHRDDSVLSFDAAVLSVSQDLCRVEGIPEEE 600

Query: 628 QSGEQDSCCGNVGSLRDSHAVSEIQEDSETGEES--GRLSVASNGTRPANQTSEFQDLKR 687
           QSGEQDSCCGNVGSL+DSHAVSEIQEDSETGEES   RLS ASNG R ANQT E +DLK 
Sbjct: 601 QSGEQDSCCGNVGSLKDSHAVSEIQEDSETGEESIPNRLSFASNGNRSANQTFEIRDLKL 660

Query: 688 SNSTTSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFS 747
           SNST +GA KDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL +G+RA+SMG  VSFS
Sbjct: 661 SNSTAAGALKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGL-DGERALSMGRRVSFS 720

Query: 748 VEYNEKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGL 807
           VEYNEKESLNEMFELGE SNAAF NEES SDGEYVDEQEWGRRE EMICRHLDHIDMLGL
Sbjct: 721 VEYNEKESLNEMFELGEASNAAFDNEESMSDGEYVDEQEWGRREPEMICRHLDHIDMLGL 780

Query: 808 NKTTLRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRG 867
           N+TTLRLRYLINWLVTSLLQLRLPGRDDVG HLVQLYGPKIKYERGAA+AFN+KESNGRG
Sbjct: 781 NRTTLRLRYLINWLVTSLLQLRLPGRDDVGTHLVQLYGPKIKYERGAAVAFNVKESNGRG 840

Query: 868 LIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLF 927
           LIHPEVVQ+LAENNGISLGVGILSHVRAVDVPKQNSGQYDL+DMALCKPM NGHNRKKLF
Sbjct: 841 LIHPEVVQRLAENNGISLGVGILSHVRAVDVPKQNSGQYDLKDMALCKPMANGHNRKKLF 900

Query: 928 FRVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSES 973
           FRVEVVTASLGFLTNF+DVYKMWAF+AKFLNPSFLEN+TLSS PE+SES
Sbjct: 901 FRVEVVTASLGFLTNFEDVYKMWAFVAKFLNPSFLENSTLSSGPETSES 947

BLAST of Lsi04G016040 vs. TAIR 10
Match: AT2G23520.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1021.9 bits (2641), Expect = 3.3e-298
Identity = 567/954 (59.43%), Postives = 715/954 (74.95%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDK-KTRRRHGSG--VTERRKSSILRQLQENKLREALEEASEDG 87
           MH  LWK + HCA LI+DK K+RRR GS   +  RRK+S+LR+L E+KLR+ALEEASE+G
Sbjct: 1   MHFPLWKQIHHCATLILDKSKSRRRDGSDSPIDVRRKASMLRKLYEDKLRDALEEASENG 60

Query: 88  SLAKSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFT 147
           SL KS+D+  E+ NQD ++ RSRSLARLHAQ+EFLRATALAA+R + +ED IP L +AF 
Sbjct: 61  SLFKSQDV--ENENQDESLGRSRSLARLHAQREFLRATALAAERAFESEDDIPELLEAFN 120

Query: 148 KFLTMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLS 207
           KFLTMYPKF+TSEK+DQLRS+EY HL +  SKVCLDYCGFGLFS++QT  +W+S  F+LS
Sbjct: 121 KFLTMYPKFETSEKVDQLRSDEYGHLLD--SKVCLDYCGFGLFSYVQTLHYWDSCTFSLS 180

Query: 208 EITANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPF 267
           EITANLSNHALYGGAE GT+EHD+KTRIMDYLNI E+EYGLVFT SRGSAF+LL+ESYPF
Sbjct: 181 EITANLSNHALYGGAEIGTVEHDLKTRIMDYLNIPESEYGLVFTGSRGSAFRLLAESYPF 240

Query: 268 HTNKKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKK 327
           HTNK+LLTMFDHESQSV+WMAQ+A+E+GAK Y+AWFKWPTL+LCS +L+K++++K+++KK
Sbjct: 241 HTNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTDLKKRLSHKKRKKK 300

Query: 328 DSVAGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFI 387
           DS  GLFVFP QSRVTG+KYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLF+P+FI
Sbjct: 301 DSAVGLFVFPAQSRVTGSKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPEFI 360

Query: 388 ITSFYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDV 447
           ITSFY+VFG DPTGFGCLLIKKSV+G+LQ+QSG+TG+G+V+I P +P Y+ DSIDGL D 
Sbjct: 361 ITSFYKVFGHDPTGFGCLLIKKSVMGNLQSQSGKTGSGIVKITPQYPLYLSDSIDGL-DG 420

Query: 448 LAGIEDDEI--NGQEDSETE-KHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDG- 507
           L G+ED +I  NG + + T+   + ++MP FSG +TS QVRDVFET++ +D N+SDRDG 
Sbjct: 421 LVGLEDHDIGTNGDKPATTDAARRGAQMPVFSGAYTSAQVRDVFETDLLED-NASDRDGT 480

Query: 508 ASTIFEEAESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPP 567
           +STIFEE ES+S+GE+MKSP FSEDESSDNS+WIDLGQSP GSD++GHL   K  SPLPP
Sbjct: 481 SSTIFEENESVSVGELMKSPAFSEDESSDNSFWIDLGQSPLGSDSAGHLNHHKIASPLPP 540

Query: 568 SWFSGKRNTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKG 627
            WF+ KR    +SPKP ++   SPM  D K          VLSFDAAV+S++Q+   +  
Sbjct: 541 FWFTSKR----QSPKPVAKSYSSPMY-DGK---------DVLSFDAAVMSVTQE---INS 600

Query: 628 IPEEEQSGEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQD 687
            P                +LR+S+ + +IQE  E  E  G +   +         S F  
Sbjct: 601 TPSR--------------NLRNSNNL-QIQEIQE--ENCGNIVYRAG--------SGF-- 660

Query: 688 LKRSNSTTSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHV 747
              SN ++S    D+K++AIRRETEGEFRLLGRR     +     GLE  D   S G  V
Sbjct: 661 --GSNGSSSKISSDMKDNAIRRETEGEFRLLGRRG----TGGRLLGLE--DEQPSRGTRV 720

Query: 748 SFSVEYNEKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDM 807
           SF++     + ++   + GE S A+  +E   SDGE  +E +W RRE E++C H+DH++M
Sbjct: 721 SFNM-----DRVSHSLDQGEASLASVYDE---SDGENPNEDDWDRREPEIVCSHIDHVNM 780

Query: 808 LGLNKTTLRLRYLINWLVTSLLQLRLPGRDDVG----VHLVQLYGPKIKYERGAAIAFNI 867
           LGLNKTT RLR+LINWLV SLLQL++P     G    ++LVQ+YGPKIKYERGAA+AFN+
Sbjct: 781 LGLNKTTSRLRFLINWLVISLLQLKVPEPGSDGSSRYMNLVQIYGPKIKYERGAAVAFNV 840

Query: 868 KESNGRGLIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDL-EDMALCKPMGN 927
           K+ + +G + PE+V KLAE  G+SLG+GILSH+R +D+P+ + G   + ED +L      
Sbjct: 841 KDKS-KGFVSPEIVLKLAEREGVSLGIGILSHIRIMDLPRNHRGGARIKEDSSLHLQREA 887

Query: 928 G-HNRKKLFFRVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPE 969
           G    K  F R EVVTASL FL+NF+DVYK+WAF+AKFLNP F    +L +V E
Sbjct: 901 GKRGGKNGFVRFEVVTASLSFLSNFEDVYKLWAFVAKFLNPGFSREGSLPTVIE 887

BLAST of Lsi04G016040 vs. TAIR 10
Match: AT4G37100.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1001.5 bits (2588), Expect = 4.7e-292
Identity = 562/966 (58.18%), Postives = 697/966 (72.15%), Query Frame = 0

Query: 28  MHLSLWKPLSHCAALIMDK---KTRRRHG--SGVTERRKSSILRQLQENKLREALEEASE 87
           MH SLWK + HCA+LI+DK     RRR G  S +  ++K++++R+L E+KLREALEEASE
Sbjct: 1   MHFSLWKQIHHCASLILDKSKSSRRRRDGSDSSLNVKKKAALIRKLYEDKLREALEEASE 60

Query: 88  DGSLAKSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDA 147
           +GSL KS+DID    N D ++ RSRSLARLHAQ+EFLRATALAA+R   +ED IP L +A
Sbjct: 61  NGSLFKSQDID--QDNGDGSLGRSRSLARLHAQREFLRATALAAERIIESEDSIPELREA 120

Query: 148 FTKFLTMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFT 207
            TKFL+MYPK+Q SEKIDQLRS+EY HLS S SKVCLDYCGFGLFS++QT  +W++  F+
Sbjct: 121 LTKFLSMYPKYQASEKIDQLRSDEYSHLSSSASKVCLDYCGFGLFSYVQTLHYWDTCTFS 180

Query: 208 LSEITANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESY 267
           LSEITANLSNHALYGGAE GT+EHDIKTRIMDYLNI ENEYGLVFTVSRGSAF+LL+ESY
Sbjct: 181 LSEITANLSNHALYGGAESGTVEHDIKTRIMDYLNIPENEYGLVFTVSRGSAFRLLAESY 240

Query: 268 PFHTNKKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKR 327
           PF +NK+LLTMFDHESQSV+WMAQ+A+E+GAK Y+AWFKWPTL+LCS +L+K+++ K+++
Sbjct: 241 PFQSNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTDLKKRLSYKKRK 300

Query: 328 KKDSVAGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPD 387
           KKDS  GLFVFP QSRVTG KYSYQWMALAQQN+WHVLLDAGSLGPKDMDSLGLSLF+P+
Sbjct: 301 KKDSAVGLFVFPAQSRVTGTKYSYQWMALAQQNHWHVLLDAGSLGPKDMDSLGLSLFRPE 360

Query: 388 FIITSFYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLV 447
           FIITSFYRVFG DPTGFGCLLIKKSV+GSLQ+QSG+TG+G+V+I P +P Y+ DS+DGL 
Sbjct: 361 FIITSFYRVFGHDPTGFGCLLIKKSVMGSLQSQSGKTGSGIVKITPEYPLYLSDSVDGL- 420

Query: 448 DVLAGIEDDEINGQEDSETEKHQE-SRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDG- 507
           D L G ED      +D   E H+  ++MPAFSG +TS QVRDVFETE+ +DN SSDRDG 
Sbjct: 421 DGLVGFEDH----NDDKTKEAHRPGTQMPAFSGAYTSAQVRDVFETELLEDNISSDRDGT 480

Query: 508 -ASTIFEEAESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLP 567
            ++TIFEE ES+S+GE+MKSP+FSEDESSDNS+WIDLGQSP GSD        K  SPLP
Sbjct: 481 TSTTIFEETESVSVGELMKSPVFSEDESSDNSFWIDLGQSPLGSDQ-----HNKIASPLP 540

Query: 568 PSWFSGKRNTRKR-SPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCV 627
           P W + KR  ++R SPKP  +   SP+          +  + VLSFDAAV+S++      
Sbjct: 541 PIWLTNKRKQKQRQSPKPIPKSYSSPL----------YDGNDVLSFDAAVMSVT------ 600

Query: 628 KGIPEEEQSGEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEF 687
                  + G   +   N  S  +   V EIQE     E  G     +NG + +N +SE 
Sbjct: 601 -------EHGTNSTPSRNRRSSSNHLHVQEIQE-----ENCGH--SFANGLKSSNISSE- 660

Query: 688 QDLKRSNSTTSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGC 747
                           +KESAIRRETEGEFRLLG R+  R       G+E  D   S G 
Sbjct: 661 ----------------IKESAIRRETEGEFRLLGGRDGGR---SRLLGVE--DEHPSKGR 720

Query: 748 HVSFSVEYNEKESLNEMFELGETSNAAFGNEE--STSDGEYVDEQ----EWGRR--ESEM 807
            VSF++E       + + E GE S A+  +E+  +TSD E  D++    EW RR  E+E+
Sbjct: 721 RVSFNME----RVSHSIVEPGEASLASVYDEDYINTSDVENGDDEGADDEWDRRDTETEI 780

Query: 808 ICRHLDHIDMLGLNKTTLRLRYLINWLVTSLLQLRLPGRDDVGVHLVQLYGPKIKYERGA 867
           +CRH+DH++MLGLNKTT RLR+LINWLV SLLQL++P      ++LVQ+YGPKIKYERGA
Sbjct: 781 VCRHIDHVNMLGLNKTTTRLRFLINWLVISLLQLQVPESGGRHMNLVQIYGPKIKYERGA 840

Query: 868 AIAFNIKESNGRGLIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALC 927
           A+AFN+++ + +G + PE+VQ+L +  G+SLG+GILSH+R VD   +N      ED AL 
Sbjct: 841 AVAFNVRDKS-KGFVSPEIVQRLGDREGVSLGIGILSHIRIVDEKPRNHRARTKEDSALH 894

Query: 928 KPMGNGHNRKKLFFRVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESS 977
                G N    F R EVVTASL FLTNF+DVYK+W F+AKFLNP F    +L +V E  
Sbjct: 901 LQNEAGKNG---FIRFEVVTASLSFLTNFEDVYKLWVFVAKFLNPGFSREGSLPTVEEEE 894

BLAST of Lsi04G016040 vs. TAIR 10
Match: AT5G66950.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 984.6 bits (2544), Expect = 5.9e-287
Identity = 551/948 (58.12%), Postives = 693/948 (73.10%), Query Frame = 0

Query: 28  MHLSLWKPLSHC-AALIMDKKTRRRHGSGVTERRKSSILRQLQENKLREALEEASEDGSL 87
           MH+SLWKP+ HC AAL++DKK+     S  + R +    R+L E+KLREALE+ASEDG L
Sbjct: 1   MHISLWKPIYHCAAALVLDKKS--SGSSSSSSRNRDVTQRKLHESKLREALEQASEDGLL 60

Query: 88  AKSRDIDCESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKF 147
            KS+D++ E  +QD+ + RSRSLARL+AQ+EFLRAT+LAA R + +E+ +P L +A T F
Sbjct: 61  VKSQDMEEEDESQDQILGRSRSLARLNAQREFLRATSLAAQRAFESEETLPELEEALTIF 120

Query: 148 LTMYPKFQTSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQTQQFWESSAFTLSEI 207
           LTMYPK+Q+SEK+D+LR++EY HL  S  KVCLDYCGFGLFS++QT  +W++  F+LSEI
Sbjct: 121 LTMYPKYQSSEKVDELRNDEYFHL--SLPKVCLDYCGFGLFSYLQTVHYWDTCTFSLSEI 180

Query: 208 TANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHT 267
           +ANLSNHA+YGGAEKG+IEHDIK RIMDYLNI ENEYGLVFTVSRGSAFKLL+ESYPFHT
Sbjct: 181 SANLSNHAIYGGAEKGSIEHDIKIRIMDYLNIPENEYGLVFTVSRGSAFKLLAESYPFHT 240

Query: 268 NKKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDS 327
           NKKLLTMFDHESQSVSWM Q AKE+GAKV SAWFKWPTLRLCS +L+K+I +K+KRKKDS
Sbjct: 241 NKKLLTMFDHESQSVSWMGQCAKEKGAKVGSAWFKWPTLRLCSMDLKKEILSKKKRKKDS 300

Query: 328 VAGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIIT 387
             GLFVFPVQSRVTG+KYSYQWMALAQQNNWHVLLDAG+LGPKDMDSLGLSLF+PDFIIT
Sbjct: 301 ATGLFVFPVQSRVTGSKYSYQWMALAQQNNWHVLLDAGALGPKDMDSLGLSLFRPDFIIT 360

Query: 388 SFYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILPIFPQYIGDSIDGLVDVLA 447
           SFYRVFG DPTGFGCLLIKKSVI  LQ+QSG+T +G+V+I P +P Y+ DS+DGL + L 
Sbjct: 361 SFYRVFGYDPTGFGCLLIKKSVISCLQSQSGKTSSGIVKITPEYPLYLSDSMDGL-EGLT 420

Query: 448 GIEDDEINGQEDSETEKHQESRMPAFSGVFTSNQVRDVFETEMEQDNNSSDRDGASTIFE 507
           GI+D   NG   +   K   +++PAFSG +TS QV+DVFET+M+ +   SDRD  S +FE
Sbjct: 421 GIQD---NGIAINGDNKALGTQLPAFSGAYTSAQVQDVFETDMDHE-IGSDRDNTSAVFE 480

Query: 508 EAESISIGEVMKSPIFSEDESSDNSYWIDLGQSPFGSDNSGHLIKQKTWSPLPPSWFSGK 567
           EAESIS+GE++KSP+FSEDESSD+S WIDLGQSP  SDN+GHL KQK  SPL       K
Sbjct: 481 EAESISVGELIKSPVFSEDESSDSSLWIDLGQSPADSDNAGHLNKQK--SPL----LVRK 540

Query: 568 RNTRKRSPKPASRLLKSPMCGDDKRMNSRHYEDSVLSFDAAVLSMSQDFGCVKGIPEEEQ 627
            + R+ SPKPAS+        ++     RH    VLSFDAAVLS+S + G       EE 
Sbjct: 541 NHKRRSSPKPASK-------ANNGSNGGRH----VLSFDAAVLSVSHEVG-------EEV 600

Query: 628 SGEQDSCCGNVGSLRDSHAVSEIQEDSETGEESGRLSVASNGTRPANQTSEFQDLKRSNS 687
             E++S    + + R    V+EI+E+ E G  S +L+  +NG                  
Sbjct: 601 IEEENSEMNQIDTSRRLR-VTEIEEEEEEG-GSSKLTAHANG------------------ 660

Query: 688 TTSGAFKDLKESAIRRETEGEFRLLGRRERSRFSERGFFGLEEGDRAISMGCHVSFSVEY 747
           ++SG    +K+SAIRRETEGEFRLLGRRE+S+++         G R +     V+     
Sbjct: 661 SSSG----IKDSAIRRETEGEFRLLGRREKSQYN---------GGRLL-----VNEDEHP 720

Query: 748 NEKESLNEMFELGETSNAAFGNEESTSDGEYVDEQEWGRRESEMICRHLDHIDMLGLNKT 807
           +++       + GE S  + G+E+   DG    E +  +RE E++CRH+DH++MLGLNKT
Sbjct: 721 SKRRVSFRSVDHGEASVISLGDEDEEEDGSNGVEWDDDQREPEIVCRHIDHVNMLGLNKT 780

Query: 808 TLRLRYLINWLVTSLLQLRLPGRDDVGVH--LVQLYGPKIKYERGAAIAFNIKESNGRGL 867
           T RLRYLINWLVTSLLQLRLP  D  G H  LVQ+YGPKIKYERG+++AFNI++    G+
Sbjct: 781 TSRLRYLINWLVTSLLQLRLPRSDSDGEHKNLVQIYGPKIKYERGSSVAFNIRDLKS-GM 840

Query: 868 IHPEVVQKLAENNGISLGVGILSHVRAVDVPKQNSGQYDLEDMALCKPMGNGHNRKKLFF 927
           +HPE+VQKLAE  GISLG+G LSH++ +D   ++S  +        KP+ +   R   F 
Sbjct: 841 VHPEIVQKLAEREGISLGIGYLSHIKIIDNRSEDSSSW--------KPV-DREGRNNGFI 867

Query: 928 RVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFLENNTLSSVPESSES 973
           RVEVVTASLGFLTNF+DVY++W F+AKFL+P F +  TL +V E  +S
Sbjct: 901 RVEVVTASLGFLTNFEDVYRLWNFVAKFLSPGFAKQGTLPTVIEEDDS 867

BLAST of Lsi04G016040 vs. TAIR 10
Match: AT5G51920.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 5.9e-77
Identity = 147/345 (42.61%), Postives = 220/345 (63.77%), Query Frame = 0

Query: 95  ESPNQDRNVRRSRSLARLHAQKEFLRATALAADRTYSTEDFIPNLFDAFTKFLTMYPKFQ 154
           E P        S +L R  AQ      + +  D  ++  + +P+  ++F+ F+  YP + 
Sbjct: 31  EHPPHSTPTVTSATLRRNFAQ---TTVSTIFPDTEFTDPNSLPSHQESFSDFIQAYPNYS 90

Query: 155 TSEKIDQLRSEEYEHLSESFSKVCLDYCGFGLFSHIQ-----------TQQFWESSAFTL 214
            + KID+LRS+ Y HL  S    CLDY G GL+S+ Q           +    ES  F++
Sbjct: 91  DTYKIDRLRSDHYFHLGLS-HYTCLDYIGIGLYSYSQLLNYDPSTYQISSSLSESPFFSV 150

Query: 215 SEITANLSNHALYGGAEKGTIEHDIKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYP 274
           S    NL    L  G ++   E+ +K RIM +L ISE +Y +VFT +R SAF+L++ESYP
Sbjct: 151 SPKIGNLKEKLLNDGGQETEFEYSMKRRIMGFLKISEEDYSMVFTANRTSAFRLVAESYP 210

Query: 275 FHTNKKLLTMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRK 334
           F++ +KLLT++D+ES++VS + + +++RGAKV +A F WP L+LCS +LRK +T  +   
Sbjct: 211 FNSKRKLLTVYDYESEAVSEINRVSEKRGAKVAAAEFSWPRLKLCSSKLRKLVTAGKNGS 270

Query: 335 KDSVAGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDF 394
           K    G++VFP+ SRVTG++Y Y WM++AQ+N WHV++DA  LGPKDMDS GLS++ PDF
Sbjct: 271 KTKKKGIYVFPLHSRVTGSRYPYLWMSVAQENGWHVMIDACGLGPKDMDSFGLSIYNPDF 330

Query: 395 IITSFYRVFGSDPTGFGCLLIKKSVIGSLQNQSGRTGTGMVRILP 429
           ++ SFY+VFG +P+GFGCL +KKS I  L++    TG GM+ ++P
Sbjct: 331 MVCSFYKVFGENPSGFGCLFVKKSTISILESS---TGPGMINLVP 368

HSP 2 Score: 116.7 bits (291), Expect = 1.0e-25
Identity = 72/185 (38.92%), Postives = 104/185 (56.22%), Query Frame = 0

Query: 779 DEQEWGRRESEMICRHLDHIDMLGLNKTTLRLRYLINWLVTSLLQLRLPGRDDVGVHLVQ 838
           D +E     S +  + LDH+D LGL  T  R R LINWLV++L +L    +      LV+
Sbjct: 386 DSEETYSFSSSVEYKGLDHVDSLGLVATGNRSRCLINWLVSALYKL----KHSTTSRLVK 445

Query: 839 LYGPKIKYERGAAIAFNIKESNGRGLIHPEVVQKLAENNGISLGVGILSHVRAVDVPKQN 898
           +YGPK+ + RG A+AFN+    G   I P +VQKLAE + ISLG   L ++         
Sbjct: 446 IYGPKVNFNRGPAVAFNLFNHKGE-KIEPFIVQKLAECSNISLGKSFLKNILF------- 505

Query: 899 SGQYDLEDMALCKPMGNGHNRKKLFFRVEVVTASLGFLTNFDDVYKMWAFIAKFLNPSFL 958
             Q D E +   +      NR     R+ V+TA+LGFL NF+DVYK+W F+A+FL+  F+
Sbjct: 506 --QEDYEGVK-DRVFEKKRNRDVDEPRISVLTAALGFLANFEDVYKLWIFVARFLDSEFV 555

Query: 959 ENNTL 964
           +  ++
Sbjct: 566 DKESV 555

BLAST of Lsi04G016040 vs. TAIR 10
Match: AT4G22980.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 9 growth stages; BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT5G51920.1); Has 520 Blast hits to 468 proteins in 130 species: Archae - 5; Bacteria - 23; Metazoa - 99; Fungi - 131; Plants - 231; Viruses - 0; Other Eukaryotes - 31 (source: NCBI BLink). )

HSP 1 Score: 220.7 bits (561), Expect = 5.2e-57
Identity = 128/325 (39.38%), Postives = 197/325 (60.62%), Query Frame = 0

Query: 106 SRSLARLHAQKEFLRATA----LAADRTYSTEDFIPNLFDAFTKFLTMYPKFQTSEKIDQ 165
           S S++    + EF   T     L  +  +++++ +P L  +F   +T +P +  + + D 
Sbjct: 24  SHSMSEKPEELEFSVTTTGTSFLTRNTKFTSQESLPRLRTSFYDLITAFPDYLQTNQADH 83

Query: 166 LRSEEYEHLSESFSKVCLDYCGFG----LFSHIQTQQFWESSAFTLSEITANLSNHALYG 225
           LRS EY++LS S S V      FG    LFS+ Q ++  ES +  L+     LS   +  
Sbjct: 84  LRSTEYQNLSSS-SHV------FGQQQPLFSYSQFREISESES-DLNHSLLTLSCKQVSS 143

Query: 226 GAEKGTIEHD------IKTRIMDYLNISENEYGLVFTVSRGSAFKLLSESYPFHTNKKLL 285
           G E  + E +      I+ RI  ++N+ E+EY ++ T  R SAFK+++E Y F TN  LL
Sbjct: 144 GKELLSFEEESRFQSRIRKRITSFMNLEESEYHMILTQDRSSAFKIVAELYSFKTNPNLL 203

Query: 286 TMFDHESQSVSWMAQSAKERGAKVYSAWFKWPTLRLCSRELRKQITNKRKRKKDSVAGLF 345
           T++++E ++V  M + ++++G K  SA F WP+  + S +L+++IT  ++R K    GLF
Sbjct: 204 TVYNYEDEAVEEMIRISEKKGIKPQSAEFSWPSTEILSEKLKRRITRSKRRGK---RGLF 263

Query: 346 VFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFKPDFIITSFYRV 405
           VFP+QS VTGA YSY WM+LA+++ WHVLLD  +LG KDM++LGLSLF+PDF+I SF  V
Sbjct: 264 VFPLQSLVTGASYSYSWMSLARESEWHVLLDTSALGSKDMETLGLSLFQPDFLICSFTEV 323

Query: 406 FG-SDPTGFGCLLIKKSVIGSLQNQ 416
            G  DP+GFGCL +KKS   +L  +
Sbjct: 324 LGQDDPSGFGCLFVKKSSSTALSEE 337

HSP 2 Score: 95.9 bits (237), Expect = 1.9e-19
Identity = 67/192 (34.90%), Postives = 101/192 (52.60%), Query Frame = 0

Query: 771 STSDGEYVDEQEWGRRESEMI-CRHLDHIDMLGLNKTTLRLRYLINWLVTSLLQLRLPGR 830
           STS  E V+ +   +++  MI  + LDH D LGL   + R + L  WL+ +L  L+ PG 
Sbjct: 378 STSSSEIVEIESSVKQDKAMIEFQGLDHADSLGLILISRRSKSLTLWLLRALRTLQHPGY 437

Query: 831 DDVGVHLVQLYGPKIKYERGAAIAFNIKESNGRGLIHPEVVQKLAENNGISLGVGILSHV 890
               + LV+LYGPK K  RG +I+FNI +  G   + P +V++LAE   I L    L   
Sbjct: 438 HQTEMPLVKLYGPKTKPSRGPSISFNIFDWQGE-KVDPLMVERLAEREKIGLRCAYLHKF 497

Query: 891 RAVDVPKQNSGQYDLEDMALCKPMGN-GHNRKKLFFRVEVVTASL-GFLTNFDDVYKMWA 950
           R                      +GN   + + +  R+ VVT  L GF+TNF+DV+K+W 
Sbjct: 498 R----------------------IGNKRRSDEAVSLRLSVVTVRLGGFMTNFEDVFKVWE 546

Query: 951 FIAKFLNPSFLE 960
           F+++FL+  F+E
Sbjct: 558 FVSRFLDADFVE 546