Lsi04G015780 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G015780 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.3e-101
Identity = 176/324 (54.32%), Postives = 238/324 (73.46%), Query Frame = 0

Query: 4   DSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLY 63
           +S   ++ LELLP+L ++ +G VERL GT V PPGLD  TGV SKDIII P+TG+SAR+Y
Sbjct: 2   ESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIY 61

Query: 64  RPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHP 123
           RP ++ P +K+PL++YFHGGAFL++S++ P YH + L  +V +A  + +SVNYRLAPEHP
Sbjct: 62  RPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTS-LNKIVNQANVIAVSVNYRLAPEHP 121

Query: 124 LPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMALQA 183
           LP AY+DSW AL+ I A          +EPW+ +      +FLVGDSAG NISHH+A +A
Sbjct: 122 LPTAYEDSWTALKNIQAI---------NEPWINDYADLDSLFLVGDSAGANISHHLAFRA 181

Query: 184 KNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDPLIN 243
           K SD T KIK  GI +I PYFWG +PIG+EI +  +K  VD WW FVCPS++G+DDP IN
Sbjct: 182 KQSDQTLKIK--GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWIN 241

Query: 244 PFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHAFH 303
           PF+DGSP + GL  ++V++ VA KDIL +RGK+YYE L KSEWKGKVEI ET+ ++H FH
Sbjct: 242 PFADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFH 301

Query: 304 MLDPTSEKAKALTKRLAFFLNEIK 323
           + +P  ++A  + + LA F+N+++
Sbjct: 302 IFEPDCDEAMEMVRCLALFINQVE 313

BLAST of Lsi04G015780 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.2e-72
Identity = 139/316 (43.99%), Postives = 198/316 (62.66%), Query Frame = 0

Query: 9   ELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLYRPTAV 68
           E+ L+L P+L +YK+G +ER LGT V P   +    V +KD++I P TGVS RLY P  V
Sbjct: 82  EIVLDLKPFLIIYKSGRIERFLGTTVIPACPE----VATKDVVIDPATGVSVRLYLPNVV 141

Query: 69  D-PPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHPLPAA 128
           D P +KLP++VYFHGG F++ ++  P YH N L  L A+A  +++S+NYRLAPE+PLPA+
Sbjct: 142 DLPSKKLPVLVYFHGGGFVIENTGSPNYH-NYLTLLAAKAGVLIVSINYRLAPEYPLPAS 201

Query: 129 YDDSWAALQWITAQSQSSADEPGHEPWLKE-----LVFLVGDSAGGNISHHMALQAKNSD 188
           YDD  A   W+ + S      P  EPWL +      + L GDSAGGN++H++A++A    
Sbjct: 202 YDDCMAGFNWVVSHSAG----PALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRADAG- 261

Query: 189 LTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDPLINPFSD 248
                 IEG+A++ PYF G EP+G+EI +       D+ W    P   G DDPLINP + 
Sbjct: 262 -----VIEGVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPVAP 321

Query: 249 GSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHAFHMLDP 308
           G+P++ GL   + VV VAG D L +RG++YYE L KS W+G+ E+ + EG  H FH+ D 
Sbjct: 322 GAPSLAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHVFHLSDY 381

Query: 309 TSEKAKALTKRLAFFL 319
           + + + A+  +L  FL
Sbjct: 382 SGDISVAMMTKLIAFL 382

BLAST of Lsi04G015780 vs. ExPASy Swiss-Prot
Match: I4DST9 (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 139/316 (43.99%), Postives = 196/316 (62.03%), Query Frame = 0

Query: 9   ELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLYRPTAV 68
           E+ L+L P+L +YK+G +ER LGT V P   +    V +KD++I P TGVS RLY P  V
Sbjct: 79  EIVLDLKPFLIIYKSGRIERFLGTTVIPACPE----VATKDVVIDPATGVSVRLYLPNVV 138

Query: 69  D-PPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHPLPAA 128
           D P +KLP++VYFHGG F++ ++  P YH N L  L A+A  +++S+NYRLAPE+PLPA+
Sbjct: 139 DLPSKKLPVLVYFHGGGFVIENTGSPNYH-NYLTLLAAKAGVLIVSINYRLAPEYPLPAS 198

Query: 129 YDDSWAALQWITAQSQSSADEPGHEPWLKE-----LVFLVGDSAGGNISHHMALQAKNSD 188
           YDD  A   W+ + S      P  EPWL +      + L GDSAGGN++H++A++A    
Sbjct: 199 YDDCMAGFNWVVSHSAG----PALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRADAG- 258

Query: 189 LTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDPLINPFSD 248
                 IEG+A++ PYF G EP+G+EI +       D+ W    P   G DDPLINP + 
Sbjct: 259 -----VIEGVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPVAP 318

Query: 249 GSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHAFHMLDP 308
           G+P + GL   + VV VAG D L +RG++YYE L KS W G+ E+ + EG  H FH+ D 
Sbjct: 319 GAPILAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWGGEAELVQHEGVGHVFHLSDY 378

Query: 309 TSEKAKALTKRLAFFL 319
           + + + A+  +L  FL
Sbjct: 379 SGDISVAMMTKLIAFL 379

BLAST of Lsi04G015780 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.0e-71
Identity = 140/321 (43.61%), Postives = 198/321 (61.68%), Query Frame = 0

Query: 5   SNSTELDLELLPYLRLYKNGVVERLLGTRVTPPG-LDTRTGVESKDIIIVPETGVSARLY 64
           + S E+D EL P LR+YK+G VER LG+   PP   D  TGV +KDI+I     +SAR+Y
Sbjct: 8   TTSKEIDRELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTISARVY 67

Query: 65  RPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHP 124
            P   +   KLP++VY+HGGAF +  SA    H   L  + ++A  +++S+ YRLAPEHP
Sbjct: 68  LPKLNNTTEKLPILVYYHGGAFCL-ESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHP 127

Query: 125 LPAAYDDSWAALQWITAQSQSSADEPGHEPWLKE-----LVFLVGDSAGGNISHHMALQA 184
           LPAAY+D W AL+W+T+ S ++      +PWL +       ++ GD++G NI+H+ AL+ 
Sbjct: 128 LPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAALRV 187

Query: 185 KNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGN-DDPLI 244
               L   ++I G+    P FWG +P+ SE  E H+K+   + WNFV P   G  D+PLI
Sbjct: 188 GAEALPGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGIDNPLI 247

Query: 245 NPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHAF 304
           NP + G+P +  L   K++V VAGKD LRDRG  YYE + +S WKG VE+ + EGE H F
Sbjct: 248 NPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGEEHCF 307

Query: 305 HMLDPTSEKAKALTKRLAFFL 319
            +  P +E +K L  R+A FL
Sbjct: 308 QIYHPETENSKDLIGRIASFL 327

BLAST of Lsi04G015780 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 9.1e-67
Identity = 139/322 (43.17%), Postives = 191/322 (59.32%), Query Frame = 0

Query: 8   TELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLYRP-- 67
           +E+  E LP+ R+YK+G VERL+GT   P  LD    V SKD+I  PE  +S RL+ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 68  -TAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHPL 127
            T +    KLPL++Y HGGA+++ S   P+YH N L  +V  A  + +SV YR APE P+
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYH-NYLTEVVKSANCLAVSVQYRRAPEDPV 122

Query: 128 PAAYDDSWAALQWITAQSQSSADEPGHEPWLKE-----LVFLVGDSAGGNISHHMALQAK 187
           PAAY+D W+A+QWI A S  S    G   W+ +      VFL GDSAGGNISHHMA++A 
Sbjct: 123 PAAYEDVWSAIQWIFAHSNGS----GPVDWINKHADFGKVFLGGDSAGGNISHHMAMKA- 182

Query: 188 NSDLTTKIKIEGIALIQPYFWGQEPIGS-EITEHHKKAEVDRWWNFVCP--SDRGNDDPL 247
             +    +KI+GIA++ P FWG +P+   ++ +   ++ +   W  +    S  G DDPL
Sbjct: 183 GKEKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPL 242

Query: 248 INPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHA 307
            N    GS    GL  DKV+V VAGKD+   +G  Y   L K EW+G VE+ E EGE+H 
Sbjct: 243 FNVNGSGSD-FSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHV 302

Query: 308 FHMLDPTSEKAKALTKRLAFFL 319
           FH+ +P S+KA    K+   F+
Sbjct: 303 FHLQNPKSDKALKFLKKFVEFI 317

BLAST of Lsi04G015780 vs. ExPASy TrEMBL
Match: A0A0A0L3I9 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639160 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.0e-165
Identity = 285/325 (87.69%), Postives = 302/325 (92.92%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MA D N+ ELD+ELLPYLRLYKNGVVERLLGTRVTPPGLD+RTGV SKDI+IVP+TGVSA
Sbjct: 1   MATDLNNPELDVELLPYLRLYKNGVVERLLGTRVTPPGLDSRTGVHSKDIVIVPDTGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPTAVDP RKLPLVVYFHGGAFLVASSAEP+YHNNCLIPL AEAQTVLLSVNYRLAP
Sbjct: 61  RLYRPTAVDPGRKLPLVVYFHGGAFLVASSAEPVYHNNCLIPLAAEAQTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQS+SSADEPGHEPWLKEL     VFLVGDSAGGNI HHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSKSSADEPGHEPWLKELVDFEKVFLVGDSAGGNICHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AKNS+L  KIKI GIALIQPYFWGQEPIGSEITEHHKKAEVD WWNFVCPSDRGNDD 
Sbjct: 181 LRAKNSNLGAKIKIVGIALIQPYFWGQEPIGSEITEHHKKAEVDSWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G++V+VIVAGKDILR+RGKLYYE LA SEWKGKVE YETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLAGERVLVIVAGKDILRERGKLYYETLANSEWKGKVEFYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHML+P+SEKAKAL KRLAFFLN+
Sbjct: 301 AFHMLNPSSEKAKALLKRLAFFLNQ 325

BLAST of Lsi04G015780 vs. ExPASy TrEMBL
Match: A0A5D3D9G5 (Putative carboxylesterase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003540 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.3e-164
Identity = 282/325 (86.77%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MA DSN+ EL +ELLPYLRLYKNG+V+RLLGTRVTPPGLD+RTGV+SKDIIIVP+TGVSA
Sbjct: 1   MATDSNNPELAVELLPYLRLYKNGIVDRLLGTRVTPPGLDSRTGVDSKDIIIVPDTGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPT VDP RKLPLVVYFHGGAFLVASSAEPIYHNNCLIPL AEA TVLLSVNYRLAP
Sbjct: 61  RLYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQSQSSAD+PGHEPWL+EL     VFLVGDSAGGNISHHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AKNS+L  KIKI GIALIQPYFWGQEPIG EI EHHK+AEVD WWNFVCPSDRGNDD 
Sbjct: 181 LRAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G+KV+VIVAGKDILR+RGKLYYE LA+SEWKGKVE YETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHML+PTSEKAKAL KRLAFFLN+
Sbjct: 301 AFHMLNPTSEKAKALVKRLAFFLNQ 325

BLAST of Lsi04G015780 vs. ExPASy TrEMBL
Match: A0A5A7VAW2 (Putative carboxylesterase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00710 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 281/325 (86.46%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MA DSN+ EL +ELLPYLRLYKNG+V+RL+GTRVTPPGLD+RTGV+SKDIIIVP+TGVSA
Sbjct: 1   MATDSNNPELAVELLPYLRLYKNGIVDRLIGTRVTPPGLDSRTGVDSKDIIIVPDTGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPT VDP RKLPLVVYFHGGAFLVASSAEPIYHNNCLIPL AEA TVLLSVNYRLAP
Sbjct: 61  RLYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQSQSSAD+PGHEPWL+EL     VFLVGDSAGGNISHHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AKNS+L  KIKI GIALIQPYFWGQEPIG EI EHHK+AEVD WWNFVCPSDRGNDD 
Sbjct: 181 LRAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G+KV+VIVAGKDILR+RGKLYYE LA+SEWKGKVE YETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHML+PTSEKAKAL KRLAFFLN+
Sbjct: 301 AFHMLNPTSEKAKALVKRLAFFLNQ 325

BLAST of Lsi04G015780 vs. ExPASy TrEMBL
Match: A0A1S3BU25 (probable carboxylesterase 2 OS=Cucumis melo OX=3656 GN=LOC103493527 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 281/325 (86.46%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MA DSN+ EL +ELLPYLRLYKNG+V+RL+GTRVTPPGLD+RTGV+SKDIIIVP+TGVSA
Sbjct: 1   MATDSNNPELAVELLPYLRLYKNGIVDRLIGTRVTPPGLDSRTGVDSKDIIIVPDTGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPT VDP RKLPLVVYFHGGAFLVASSAEPIYHNNCLIPL AEA TVLLSVNYRLAP
Sbjct: 61  RLYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQSQSSAD+PGHEPWL+EL     VFLVGDSAGGNISHHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AKNS+L  KIKI GIALIQPYFWGQEPIG EI EHHK+AEVD WWNFVCPSDRGNDD 
Sbjct: 181 LRAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G+KV+VIVAGKDILR+RGKLYYE LA+SEWKGKVE YETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHML+PTSEKAKAL KRLAFFLN+
Sbjct: 301 AFHMLNPTSEKAKALVKRLAFFLNQ 325

BLAST of Lsi04G015780 vs. ExPASy TrEMBL
Match: A0A6J1J426 (probable carboxylesterase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481148 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 257/327 (78.59%), Postives = 289/327 (88.38%), Query Frame = 0

Query: 1   MAADSNS--TELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGV 60
           +A DSN+   EL+LELLPYLR+YKNGVVERL+GTRVTPPGLD+ TGV+SKDI IVPETG+
Sbjct: 2   VAPDSNNPELELELELLPYLRVYKNGVVERLVGTRVTPPGLDSLTGVDSKDITIVPETGI 61

Query: 61  SARLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRL 120
           SARLYRPTAV+PPRKLPL+VYFHGGAFL+ SSAEPIYHNNCLIPL A  ++V+LSVNYRL
Sbjct: 62  SARLYRPTAVEPPRKLPLIVYFHGGAFLITSSAEPIYHNNCLIPLAAATESVVLSVNYRL 121

Query: 121 APEHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHH 180
           APEHPLPAAYDDSWAALQW+  QS++S DE G EPWLK+L     VFL GDSAGGNI+HH
Sbjct: 122 APEHPLPAAYDDSWAALQWVAEQSKTSGDEAGQEPWLKQLVDFKKVFLFGDSAGGNIAHH 181

Query: 181 MALQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGND 240
           MAL+A+NS L  K KI GIALIQPYFWG+EPIGSEITE  KKA VD WWNFVCPSDRGND
Sbjct: 182 MALRAQNSHLAGKFKIRGIALIQPYFWGREPIGSEITEPEKKALVDGWWNFVCPSDRGND 241

Query: 241 DPLINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGE 300
           D LINPF+DGSP+++GLVG+KVVVIVA KDILRDRG LYYENLAKS WKGKV+I+ETEGE
Sbjct: 242 DLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLAKSHWKGKVDIFETEGE 301

Query: 301 NHAFHMLDPTSEKAKALTKRLAFFLNE 321
           +HAFH+LDPTSEKAK L  RLA F+NE
Sbjct: 302 DHAFHLLDPTSEKAKCLVNRLALFVNE 328

BLAST of Lsi04G015780 vs. NCBI nr
Match: XP_038898530.1 (probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 3.8e-171
Identity = 297/325 (91.38%), Postives = 309/325 (95.08%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MAADSN+ ELDLELLPYLRLY+NGVVERLLGTR+TPPGLDTRTGV+SKDIIIVPETGVSA
Sbjct: 1   MAADSNNPELDLELLPYLRLYRNGVVERLLGTRITPPGLDTRTGVDSKDIIIVPETGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPTAVDP RKLPLVVYFHGGAFLVASSAEPIYHNNCLIPL AEAQTVLLSVNYRLAP
Sbjct: 61  RLYRPTAVDPHRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAQTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQSQ+SADEPGHEPWLKEL     VFLVGDSAGGNISHHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSQTSADEPGHEPWLKELVDFEKVFLVGDSAGGNISHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AK+S+L  KIK+ GIALIQPYFWGQEPIGSEITEHHK+AEVDRWWNFVCPSDRGNDD 
Sbjct: 181 LRAKSSNLGAKIKVVGIALIQPYFWGQEPIGSEITEHHKRAEVDRWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G+KVVVIVA KDILRDRGKLYYENLAKSEWKGKVEIYETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLTGEKVVVIVAEKDILRDRGKLYYENLAKSEWKGKVEIYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHMLDPTSEKAKAL KRLAFFLNE
Sbjct: 301 AFHMLDPTSEKAKALIKRLAFFLNE 325

BLAST of Lsi04G015780 vs. NCBI nr
Match: XP_011654101.1 (probable carboxylesterase 2 [Cucumis sativus] >KGN55177.1 hypothetical protein Csa_012076 [Cucumis sativus])

HSP 1 Score: 591.7 bits (1524), Expect = 4.1e-165
Identity = 285/325 (87.69%), Postives = 302/325 (92.92%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MA D N+ ELD+ELLPYLRLYKNGVVERLLGTRVTPPGLD+RTGV SKDI+IVP+TGVSA
Sbjct: 1   MATDLNNPELDVELLPYLRLYKNGVVERLLGTRVTPPGLDSRTGVHSKDIVIVPDTGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPTAVDP RKLPLVVYFHGGAFLVASSAEP+YHNNCLIPL AEAQTVLLSVNYRLAP
Sbjct: 61  RLYRPTAVDPGRKLPLVVYFHGGAFLVASSAEPVYHNNCLIPLAAEAQTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQS+SSADEPGHEPWLKEL     VFLVGDSAGGNI HHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSKSSADEPGHEPWLKELVDFEKVFLVGDSAGGNICHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AKNS+L  KIKI GIALIQPYFWGQEPIGSEITEHHKKAEVD WWNFVCPSDRGNDD 
Sbjct: 181 LRAKNSNLGAKIKIVGIALIQPYFWGQEPIGSEITEHHKKAEVDSWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G++V+VIVAGKDILR+RGKLYYE LA SEWKGKVE YETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLAGERVLVIVAGKDILRERGKLYYETLANSEWKGKVEFYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHML+P+SEKAKAL KRLAFFLN+
Sbjct: 301 AFHMLNPSSEKAKALLKRLAFFLNQ 325

BLAST of Lsi04G015780 vs. NCBI nr
Match: TYK20217.1 (putative carboxylesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 586.3 bits (1510), Expect = 1.7e-163
Identity = 282/325 (86.77%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MA DSN+ EL +ELLPYLRLYKNG+V+RLLGTRVTPPGLD+RTGV+SKDIIIVP+TGVSA
Sbjct: 1   MATDSNNPELAVELLPYLRLYKNGIVDRLLGTRVTPPGLDSRTGVDSKDIIIVPDTGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPT VDP RKLPLVVYFHGGAFLVASSAEPIYHNNCLIPL AEA TVLLSVNYRLAP
Sbjct: 61  RLYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQSQSSAD+PGHEPWL+EL     VFLVGDSAGGNISHHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AKNS+L  KIKI GIALIQPYFWGQEPIG EI EHHK+AEVD WWNFVCPSDRGNDD 
Sbjct: 181 LRAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G+KV+VIVAGKDILR+RGKLYYE LA+SEWKGKVE YETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHML+PTSEKAKAL KRLAFFLN+
Sbjct: 301 AFHMLNPTSEKAKALVKRLAFFLNQ 325

BLAST of Lsi04G015780 vs. NCBI nr
Match: XP_008452527.1 (PREDICTED: probable carboxylesterase 2 [Cucumis melo] >KAA0064370.1 putative carboxylesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 585.5 bits (1508), Expect = 2.9e-163
Identity = 281/325 (86.46%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 1   MAADSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSA 60
           MA DSN+ EL +ELLPYLRLYKNG+V+RL+GTRVTPPGLD+RTGV+SKDIIIVP+TGVSA
Sbjct: 1   MATDSNNPELAVELLPYLRLYKNGIVDRLIGTRVTPPGLDSRTGVDSKDIIIVPDTGVSA 60

Query: 61  RLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAP 120
           RLYRPT VDP RKLPLVVYFHGGAFLVASSAEPIYHNNCLIPL AEA TVLLSVNYRLAP
Sbjct: 61  RLYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAP 120

Query: 121 EHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMA 180
           EHPLPAAYDDSWAALQWI AQSQSSAD+PGHEPWL+EL     VFLVGDSAGGNISHHMA
Sbjct: 121 EHPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMA 180

Query: 181 LQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDP 240
           L+AKNS+L  KIKI GIALIQPYFWGQEPIG EI EHHK+AEVD WWNFVCPSDRGNDD 
Sbjct: 181 LRAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDL 240

Query: 241 LINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENH 300
           LINPFSDGSPAIDGL G+KV+VIVAGKDILR+RGKLYYE LA+SEWKGKVE YETEGE+H
Sbjct: 241 LINPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDH 300

Query: 301 AFHMLDPTSEKAKALTKRLAFFLNE 321
           AFHML+PTSEKAKAL KRLAFFLN+
Sbjct: 301 AFHMLNPTSEKAKALVKRLAFFLNQ 325

BLAST of Lsi04G015780 vs. NCBI nr
Match: XP_022982269.1 (probable carboxylesterase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 539.3 bits (1388), Expect = 2.4e-149
Identity = 257/327 (78.59%), Postives = 289/327 (88.38%), Query Frame = 0

Query: 1   MAADSNS--TELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGV 60
           +A DSN+   EL+LELLPYLR+YKNGVVERL+GTRVTPPGLD+ TGV+SKDI IVPETG+
Sbjct: 2   VAPDSNNPELELELELLPYLRVYKNGVVERLVGTRVTPPGLDSLTGVDSKDITIVPETGI 61

Query: 61  SARLYRPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRL 120
           SARLYRPTAV+PPRKLPL+VYFHGGAFL+ SSAEPIYHNNCLIPL A  ++V+LSVNYRL
Sbjct: 62  SARLYRPTAVEPPRKLPLIVYFHGGAFLITSSAEPIYHNNCLIPLAAATESVVLSVNYRL 121

Query: 121 APEHPLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHH 180
           APEHPLPAAYDDSWAALQW+  QS++S DE G EPWLK+L     VFL GDSAGGNI+HH
Sbjct: 122 APEHPLPAAYDDSWAALQWVAEQSKTSGDEAGQEPWLKQLVDFKKVFLFGDSAGGNIAHH 181

Query: 181 MALQAKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGND 240
           MAL+A+NS L  K KI GIALIQPYFWG+EPIGSEITE  KKA VD WWNFVCPSDRGND
Sbjct: 182 MALRAQNSHLAGKFKIRGIALIQPYFWGREPIGSEITEPEKKALVDGWWNFVCPSDRGND 241

Query: 241 DPLINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGE 300
           D LINPF+DGSP+++GLVG+KVVVIVA KDILRDRG LYYENLAKS WKGKV+I+ETEGE
Sbjct: 242 DLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLAKSHWKGKVDIFETEGE 301

Query: 301 NHAFHMLDPTSEKAKALTKRLAFFLNE 321
           +HAFH+LDPTSEKAK L  RLA F+NE
Sbjct: 302 DHAFHLLDPTSEKAKCLVNRLALFVNE 328

BLAST of Lsi04G015780 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 2.4e-102
Identity = 176/324 (54.32%), Postives = 238/324 (73.46%), Query Frame = 0

Query: 4   DSNSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLY 63
           +S   ++ LELLP+L ++ +G VERL GT V PPGLD  TGV SKDIII P+TG+SAR+Y
Sbjct: 2   ESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIY 61

Query: 64  RPTAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHP 123
           RP ++ P +K+PL++YFHGGAFL++S++ P YH + L  +V +A  + +SVNYRLAPEHP
Sbjct: 62  RPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTS-LNKIVNQANVIAVSVNYRLAPEHP 121

Query: 124 LPAAYDDSWAALQWITAQSQSSADEPGHEPWLKEL-----VFLVGDSAGGNISHHMALQA 183
           LP AY+DSW AL+ I A          +EPW+ +      +FLVGDSAG NISHH+A +A
Sbjct: 122 LPTAYEDSWTALKNIQAI---------NEPWINDYADLDSLFLVGDSAGANISHHLAFRA 181

Query: 184 KNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHHKKAEVDRWWNFVCPSDRGNDDPLIN 243
           K SD T KIK  GI +I PYFWG +PIG+EI +  +K  VD WW FVCPS++G+DDP IN
Sbjct: 182 KQSDQTLKIK--GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWIN 241

Query: 244 PFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHAFH 303
           PF+DGSP + GL  ++V++ VA KDIL +RGK+YYE L KSEWKGKVEI ET+ ++H FH
Sbjct: 242 PFADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFH 301

Query: 304 MLDPTSEKAKALTKRLAFFLNEIK 323
           + +P  ++A  + + LA F+N+++
Sbjct: 302 IFEPDCDEAMEMVRCLALFINQVE 313

BLAST of Lsi04G015780 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 255.4 bits (651), Expect = 6.5e-68
Identity = 139/322 (43.17%), Postives = 191/322 (59.32%), Query Frame = 0

Query: 8   TELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLYRP-- 67
           +E+  E LP+ R+YK+G VERL+GT   P  LD    V SKD+I  PE  +S RL+ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 68  -TAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHPL 127
            T +    KLPL++Y HGGA+++ S   P+YH N L  +V  A  + +SV YR APE P+
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYH-NYLTEVVKSANCLAVSVQYRRAPEDPV 122

Query: 128 PAAYDDSWAALQWITAQSQSSADEPGHEPWLKE-----LVFLVGDSAGGNISHHMALQAK 187
           PAAY+D W+A+QWI A S  S    G   W+ +      VFL GDSAGGNISHHMA++A 
Sbjct: 123 PAAYEDVWSAIQWIFAHSNGS----GPVDWINKHADFGKVFLGGDSAGGNISHHMAMKA- 182

Query: 188 NSDLTTKIKIEGIALIQPYFWGQEPIGS-EITEHHKKAEVDRWWNFVCP--SDRGNDDPL 247
             +    +KI+GIA++ P FWG +P+   ++ +   ++ +   W  +    S  G DDPL
Sbjct: 183 GKEKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPL 242

Query: 248 INPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHA 307
            N    GS    GL  DKV+V VAGKD+   +G  Y   L K EW+G VE+ E EGE+H 
Sbjct: 243 FNVNGSGSD-FSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHV 302

Query: 308 FHMLDPTSEKAKALTKRLAFFL 319
           FH+ +P S+KA    K+   F+
Sbjct: 303 FHLQNPKSDKALKFLKKFVEFI 317

BLAST of Lsi04G015780 vs. TAIR 10
Match: AT1G49650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.6e-66
Identity = 139/324 (42.90%), Postives = 190/324 (58.64%), Query Frame = 0

Query: 6   NSTELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLYRP 65
           +S+E+  E  P++R+YK+G +ERL GT   P  L+ R  V SKD++  P   +S RL+ P
Sbjct: 57  SSSEIISEHPPFVRVYKDGRIERLSGTETVPASLNPRNDVVSKDVVYSPGHNLSVRLFLP 116

Query: 66  ---TAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEH 125
              T +    KLPL++YFHGGA++  S   PIYH N L  +V  A  + +SV YR APE 
Sbjct: 117 HKSTQLAAGNKLPLLIYFHGGAWINESPFSPIYH-NFLTEVVKSANCLAVSVQYRRAPED 176

Query: 126 PLPAAYDDSWAALQWITAQSQSSADEPGHEPWLK-----ELVFLVGDSAGGNISHHMALQ 185
           P+PAAY+D+W+A+QWI + S  S    G E W+      E VFL GDSAGGNISHHMA++
Sbjct: 177 PVPAAYEDTWSAIQWIFSHSCGS----GEEDWINKYADFERVFLAGDSAGGNISHHMAMR 236

Query: 186 AKNSDLTTKIKIEGIALIQPYFWGQEPIGSEITEHH--KKAEVDRWWNFVCP-SDRGNDD 245
           A    L  K +I+G  ++ P  WG++P+     +    +    + W   V P S  G DD
Sbjct: 237 AGKEKL--KPRIKGTVIVHPAIWGKDPVDEHDVQDREIRDGVAEVWEKIVSPNSVDGADD 296

Query: 246 PLINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGEN 305
           P  N    GS    G+  DKV+V VAGKD+   +G  Y   L KS WKG+VE+ E E E 
Sbjct: 297 PWFNVVGSGS-NFSGMGCDKVLVEVAGKDVFWRQGLAYAAKLKKSGWKGEVEVIEEEDEE 356

Query: 306 HAFHMLDPTSEKAKALTKRLAFFL 319
           H FH+L+P+SE A +  KR   F+
Sbjct: 357 HCFHLLNPSSENAPSFMKRFVEFI 372

BLAST of Lsi04G015780 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 248.4 bits (633), Expect = 7.9e-66
Identity = 135/328 (41.16%), Postives = 189/328 (57.62%), Query Frame = 0

Query: 8   TELDLELLPYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLYRP-- 67
           +E+  +  P L +YK+G +ERL+G    PP  + + GV SKD++  P+  +S R+Y P  
Sbjct: 3   SEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEK 62

Query: 68  ---TAVDPPRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEH 127
                 +   KLPL+VYFHGG FLV ++  P YH   L   V+ +  V +SV+YR APEH
Sbjct: 63  AATAETEASVKLPLLVYFHGGGFLVETAFSPTYH-TFLTAAVSASDCVAVSVDYRRAPEH 122

Query: 128 PLPAAYDDSWAALQWITAQSQSSADEPGHEPWLKE-----LVFLVGDSAGGNISHHMALQ 187
           P+P +YDDSW AL+W+ +    S    G E WL +      VFL GDSAG NI+HHM ++
Sbjct: 123 PIPTSYDDSWTALKWVFSHIAGS----GSEDWLNKHADFSKVFLAGDSAGANITHHMTMK 182

Query: 188 AKNSDLT----TKIKIEGIALIQPYFWGQEPI-GSEITEHHKKAEVDRWWNFVCPSDR-G 247
           A    L+     +  I GI L+ PYFW + P+   E T+   +  ++  W    P+ + G
Sbjct: 183 AAKDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDG 242

Query: 248 NDDPLINPFSDGSPAIDGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKV-EIYET 307
           +DDP IN     S  + GL   KV+V+VA KD L  +G  Y+E L KS W G+V ++ ET
Sbjct: 243 SDDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVET 302

Query: 308 EGENHAFHMLDPTSEKAKALTKRLAFFL 319
           +GE H FH+ DP SEKA  L  R A F+
Sbjct: 303 KGEGHVFHLRDPNSEKAHELVHRFAGFI 325

BLAST of Lsi04G015780 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.1e-64
Identity = 135/313 (43.13%), Postives = 186/313 (59.42%), Query Frame = 0

Query: 16  PYLRLYKNGVVERLLGTRVTPPGLDTRTGVESKDIIIVPETGVSARLYRPTAVDPPRKLP 75
           P  R+YK+G +ERLLG    PP L  + GV SKDII  PE  +S R+Y P  V   +KLP
Sbjct: 11  PMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKV-TVKKLP 70

Query: 76  LVVYFHGGAFLVASSAEPIYHNNCLIPLVAEAQTVLLSVNYRLAPEHPLPAAYDDSWAAL 135
           +++YFHGG F++ ++  P YH   L   VA A  + +SVNYR APE P+P  Y+DSW +L
Sbjct: 71  ILIYFHGGGFIIETAFSPPYH-TFLTSAVAAANCLAISVNYRRAPEFPVPIPYEDSWDSL 130

Query: 136 QWITAQSQSSADEPGHEPWLKE-----LVFLVGDSAGGNISHHMALQAKNSDLTTKIKIE 195
           +W+      +    G E W+ +      VFL GDSAGGNISHH+ ++AK   L   + I 
Sbjct: 131 KWVLTHITGT----GPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSL-IS 190

Query: 196 GIALIQPYFWGQEPIGS-EITEHHKKAEVDRWWNFVCP-SDRGNDDPLINPF-SDGSPAI 255
           GI LI PYFW + PI   E+ +  K   V+  W    P S +G DDP +N   SD S   
Sbjct: 191 GIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPS--- 250

Query: 256 DGLVGDKVVVIVAGKDILRDRGKLYYENLAKSEWKGKVEIYETEGENHAFHMLDPTSEKA 315
            GL   +V+V+VAG D+   +G  Y E L KS W+G+VE+ ET+ E H FH+ +P S+ A
Sbjct: 251 -GLGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNA 310

Query: 316 KALTKRLAFFLNE 321
           + + K+L  F+N+
Sbjct: 311 RQVVKKLEEFINK 312