Lsi04G015650 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G015650 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.1e-162
Identity = 264/332 (79.52%), Postives = 294/332 (88.55%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           +ELKSLIAA H+KGIK +ADIVINHRTAE++D RGI+CIFEGGTPDSR DWGPSFIC DD
Sbjct: 90  NELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSFICRDD 149

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           T YSDG GN DSGE Y  APDIDHLNPQVQ+E+SEWM WLKTEIGFDGWRFDFVKGYAPS
Sbjct: 150 TAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVKGYAPS 209

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           I+K+YM+QT PDFAVGEKWDS+S GQD KPN NQD+HR AL NWV ++G  +TAFDFTTK
Sbjct: 210 ISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAFDFTTK 269

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAVQGELWRL D NGKPPG IG+KPE+AVTFIDNHDTGSTQR+WPFPSDKV+QGYA
Sbjct: 270 GILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDKVMQGYA 329

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTPSIFYDHF DWGLKE+I KLS+IR RNGI + STV IMASEGDLYVA +D K
Sbjct: 330 YILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYVAKIDNK 389

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWE 415
           I++KIG   +LGNLIPS+  +ATSG +YAVWE
Sbjct: 390 IMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

BLAST of Lsi04G015650 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 470.7 bits (1210), Expect = 1.7e-131
Identity = 213/334 (63.77%), Postives = 259/334 (77.54%), Query Frame = 0

Query: 84  ELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDT 143
           +LKSLI ALH KG+KAIADIVINHRTAE +D RGI+CIFEGGTPD+RLDWGP  IC DD 
Sbjct: 93  QLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGGTPDARLDWGPHMICRDDR 152

Query: 144 EYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSI 203
            Y+DG GNPD+G  +  APDIDHLN +VQKE+ EW+ WLK +IGFDGWRFDF KGY+  +
Sbjct: 153 PYADGTGNPDTGADFGAAPDIDHLNLRVQKELVEWLNWLKADIGFDGWRFDFAKGYSADV 212

Query: 204 TKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASG--DTVTAFDFTT 263
            K+Y+D++ P FAV E W SL+ G D KPN NQD HR  L NWV   G     T FDFTT
Sbjct: 213 AKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELVNWVDKVGGKGPATTFDFTT 272

Query: 264 KGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGY 323
           KGIL  AV+GELWRL+ ++GK PG IG  P  AVTF+DNHDTGSTQ MWPFPSD+V+QGY
Sbjct: 273 KGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGY 332

Query: 324 AYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDG 383
           AYILTHPGTP IFYDHF DWGLKEEI +L ++R R+GI   S + I+ ++ DLY+A +DG
Sbjct: 333 AYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDG 392

Query: 384 KIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           K+I+K+G   ++GNLIP  +++A  G +YAVWE+
Sbjct: 393 KVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVWEK 426

BLAST of Lsi04G015650 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.2e-129
Identity = 203/332 (61.14%), Postives = 254/332 (76.51%), Query Frame = 0

Query: 84  ELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDT 143
           ELKSLI A H K ++ +ADIVINHR A+ +D RG++C+FEGGTPD RLDWGP  ICSDDT
Sbjct: 94  ELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPDMICSDDT 153

Query: 144 EYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSI 203
           +YS+G+G+ D+G  +  APDIDHLNP+VQ+E+++W+ WL+T++GFDGWR DF KGY+  +
Sbjct: 154 QYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDFAKGYSAPL 213

Query: 204 TKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTKG 263
            ++Y+D T P F VGE W SL    D KP+ NQDA R  L NWV   G   TAFDFTTKG
Sbjct: 214 ARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPATAFDFTTKG 273

Query: 264 ILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAY 323
           ILQAAVQGELWRL D NGK PG +G  P+ AVTF+DNHDTGSTQ +WPFPSDKV+QGYAY
Sbjct: 274 ILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSDKVMQGYAY 333

Query: 324 ILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGKI 383
           ILTHPG P IFYDH  DW L+ EI  L+ IR+RNGI   ST++I+ +EGD+YVA +DGK+
Sbjct: 334 ILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDIYVAMIDGKV 393

Query: 384 IMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           I K+G   + G +IPSD+ +   G +Y VWE+
Sbjct: 394 ITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEK 425

BLAST of Lsi04G015650 vs. ExPASy Swiss-Prot
Match: P27939 (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 463.8 bits (1192), Expect = 2.1e-129
Identity = 207/333 (62.16%), Postives = 255/333 (76.58%), Query Frame = 0

Query: 84  ELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDT 143
           EL+SLIAA H K IK +ADIVINHR A+ +D RGI+CIFEGGTPDSRLDWGP  ICSDDT
Sbjct: 95  ELRSLIAAFHSKSIKCVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDWGPDMICSDDT 154

Query: 144 EYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSI 203
           +YS+G+G+ D+G  +  APDIDHLN +VQ E+S+W+ WLK+++GFDGWR DF KGY+ ++
Sbjct: 155 QYSNGRGHRDTGADFGAAPDIDHLNTRVQTELSDWLNWLKSDVGFDGWRLDFAKGYSATV 214

Query: 204 TKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTKG 263
            K Y+D T P F V E W ++    + +P+ NQD  R  L NW +A G   +AFDFTTKG
Sbjct: 215 AKTYVDNTDPSFVVAEIWSNMRYDGNGEPSWNQDGDRQELVNWAQAVGGPASAFDFTTKG 274

Query: 264 ILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAY 323
            LQAAVQGELWR+KD NGK PG IG  PE AVTFIDNHDTGSTQ  WPFPSDKV+QGYAY
Sbjct: 275 ELQAAVQGELWRMKDGNGKAPGMIGWLPEKAVTFIDNHDTGSTQNSWPFPSDKVMQGYAY 334

Query: 324 ILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGKI 383
           ILTHPG P IFYDH  DW LK+EI  L+A+R+RNGI   S +NI+A++GD+YVA +D K+
Sbjct: 335 ILTHPGVPCIFYDHVFDWNLKQEISTLAAVRSRNGIHPGSKLNILAADGDVYVAMIDDKV 394

Query: 384 IMKIGANTNLGNLIPSDYQLATSGMEYAVWERN 417
           I KIG   ++GNLIPSD+ +   G  Y VWE++
Sbjct: 395 ITKIGTRYDVGNLIPSDFHVVAHGNNYCVWEKS 427

BLAST of Lsi04G015650 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 463.4 bits (1191), Expect = 2.7e-129
Identity = 204/332 (61.45%), Postives = 252/332 (75.90%), Query Frame = 0

Query: 84  ELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDT 143
           ELKSLIAA H KG++ +AD+VINHR AEK+D RG++C+FEGGTPD RLDWGP  ICSDDT
Sbjct: 94  ELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEGGTPDDRLDWGPGMICSDDT 153

Query: 144 EYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSI 203
           +YSDG G+ D+GE +  APDIDHLNP+VQ+E+++W+ WLK+++GFDGWR DF KGY+  I
Sbjct: 154 QYSDGTGHRDTGEGFGAAPDIDHLNPRVQRELTDWLNWLKSDVGFDGWRLDFAKGYSTDI 213

Query: 204 TKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTKG 263
            K+Y++   P F V E W+SLS   D KP  NQD  R  L NWV A G     FDFTTKG
Sbjct: 214 AKMYVESCKPGFVVAEIWNSLSYNGDGKPAANQDQGRQELVNWVNAVGGPAMTFDFTTKG 273

Query: 264 ILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAY 323
           +LQA VQGELWRL+D NGK  G IG  PE AVTF+DNHDTGSTQ++WPFPSDKV+QGYAY
Sbjct: 274 LLQAGVQGELWRLRDGNGKAAGMIGWLPEKAVTFVDNHDTGSTQKLWPFPSDKVMQGYAY 333

Query: 324 ILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGKI 383
           ILTHPG P IFYDH  DW LK+EI  L+AIR RNGI   S + I+ ++ D YVA VD K+
Sbjct: 334 ILTHPGVPCIFYDHMFDWNLKQEITALAAIRERNGINAGSKLRIVVADADAYVAVVDEKV 393

Query: 384 IMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           ++KIG   ++GN +PSD+     G +Y+VWE+
Sbjct: 394 MVKIGTRYDVGNAVPSDFHQTVHGKDYSVWEK 425

BLAST of Lsi04G015650 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 4.0e-184
Identity = 302/333 (90.69%), Postives = 323/333 (97.00%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           +ELKSLIAALH+KGIKAIADIVINHRTAEKQD+RGIWCIFEGGTPD+RLDWGPSFICSDD
Sbjct: 105 NELKSLIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICSDD 164

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           T+YSDGKGNPDSGEPYPPAPDIDHL PQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS
Sbjct: 165 TDYSDGKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 224

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           +TKLYMDQT PDFAVGEKWDSLS+G D K NQNQDAHRD+LANWV+ASGD VTAFDFTTK
Sbjct: 225 VTKLYMDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFTTK 284

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTF+DNHDTGSTQ++WPFPSDKVIQGYA
Sbjct: 285 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQGYA 344

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTP+IFYDHFV+WGLKEEI++LS IRARN IGKTS VNI+AS+ DLYVAGVDGK
Sbjct: 345 YILTHPGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVDGK 404

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIGANTNLGNLIPS++QLATSGMEYAVWE+
Sbjct: 405 IIMKIGANTNLGNLIPSEFQLATSGMEYAVWEK 437

BLAST of Lsi04G015650 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.2e-182
Identity = 300/333 (90.09%), Postives = 316/333 (94.89%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           DELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEGGTPDSRLDWGPSFICSDD
Sbjct: 92  DELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 151

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           TEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP 
Sbjct: 152 TEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPK 211

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALANWVR SGDTVTAFDFTTK
Sbjct: 212 ITKLYMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTK 271

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAVQGELWRLKD NGKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYA
Sbjct: 272 GILQAAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYA 331

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTPSIFYDHFV+WGLKEE++KLSAIR RNGIG+ STVNIMAS+GDLYVAG+DGK
Sbjct: 332 YILTHPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGK 391

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIG NTNLGNLIPSDY+LATSGM+YAVWE+
Sbjct: 392 IIMKIGPNTNLGNLIPSDYELATSGMQYAVWEK 424

BLAST of Lsi04G015650 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.9e-182
Identity = 299/333 (89.79%), Postives = 317/333 (95.20%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           +ELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEGGTPDSRLDWGPSFICSDD
Sbjct: 116 NELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 175

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           TEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP 
Sbjct: 176 TEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPK 235

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALANWVR SGDTVTAFDFTTK
Sbjct: 236 ITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTK 295

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYA
Sbjct: 296 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYA 355

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTPSIFYDHFV+WGLKEE++KLSAIRARNGIG+ STVNIMAS+GDLYVAG+DGK
Sbjct: 356 YILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGK 415

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIG NTNLGNL+PSDY+LA SGM+YAVWE+
Sbjct: 416 IIMKIGPNTNLGNLVPSDYELAASGMQYAVWEK 448

BLAST of Lsi04G015650 vs. ExPASy TrEMBL
Match: A0A6A1W532 (Alpha-amylase OS=Morella rubra OX=262757 GN=CJ030_MR3G003095 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.9e-164
Identity = 270/333 (81.08%), Postives = 298/333 (89.49%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           DELKSLI+ALH+KGIKAIADIVINHRTAEKQD RGIWCIFEGGTPD+RLDWGPSFIC DD
Sbjct: 120 DELKSLISALHEKGIKAIADIVINHRTAEKQDGRGIWCIFEGGTPDARLDWGPSFICKDD 179

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           T+YSDG GN DSGE + PAPDIDHLNPQVQKE+S+WM WLKTEIGF GWRFDFVKGYAPS
Sbjct: 180 TDYSDGSGNLDSGEDFRPAPDIDHLNPQVQKELSDWMNWLKTEIGFSGWRFDFVKGYAPS 239

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           ITK+YM+QT PDFAVGE W+SL+     KPN NQDAHR ALANWV+ +G  VTAFDFTTK
Sbjct: 240 ITKIYMEQTTPDFAVGENWNSLAYDDGGKPNPNQDAHRGALANWVQGAGGAVTAFDFTTK 299

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAV+GELWRLKDSNGKPPG IG KPESAVTFIDNHDTGSTQ++WPFPSDKV+QGYA
Sbjct: 300 GILQAAVEGELWRLKDSNGKPPGLIGTKPESAVTFIDNHDTGSTQKLWPFPSDKVMQGYA 359

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPG P+IFYDHF DWGLKEEI KLSAIR+RNGI  TS VNI+AS+ DLY+A VD K
Sbjct: 360 YILTHPGIPTIFYDHFFDWGLKEEISKLSAIRSRNGISSTSRVNILASDADLYLAEVDNK 419

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIG   +LGNL+PS+YQ+ATSG +YAVWE+
Sbjct: 420 IIMKIGPKMDLGNLVPSNYQVATSGNDYAVWEK 452

BLAST of Lsi04G015650 vs. ExPASy TrEMBL
Match: A0A2I4EXA7 (Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 8.7e-163
Identity = 269/342 (78.65%), Postives = 300/342 (87.72%), Query Frame = 0

Query: 74  LGIKQQRRMDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDW 133
           L + +    DELK+LIAALH+KGIK +ADIVINHRTAEKQD+RGIW IFEGGTPD+RLDW
Sbjct: 81  LDVSKYGSKDELKALIAALHEKGIKVVADIVINHRTAEKQDDRGIWSIFEGGTPDARLDW 140

Query: 134 GPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRF 193
           GPSFIC DD  YSDG GNPDSGE Y PAPDIDH NPQVQKE+SEWM WLKTEIG+DGWRF
Sbjct: 141 GPSFICKDDIAYSDGTGNPDSGEGYDPAPDIDHRNPQVQKELSEWMNWLKTEIGYDGWRF 200

Query: 194 DFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDT 253
           DFVKGYAPSI K YM+QT PDFAVGE W+SL+ G D KPN NQDAHR ALA WV ++G  
Sbjct: 201 DFVKGYAPSIMKNYMEQTKPDFAVGENWNSLAYGDDGKPNPNQDAHRGALAEWVGSAGGA 260

Query: 254 VTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFP 313
           VTAFDFTTKGILQAAV+GELWRLKDSNGKPPG IGIKPESAVTFIDNHDTGSTQ +WPFP
Sbjct: 261 VTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPESAVTFIDNHDTGSTQNIWPFP 320

Query: 314 SDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGD 373
           SDKV+QGYAYILTHPGTP+IFYDHF DWGLKEEI KL+AIR+RNGI   S V+I+AS+GD
Sbjct: 321 SDKVMQGYAYILTHPGTPTIFYDHFFDWGLKEEISKLTAIRSRNGINTRSIVSILASDGD 380

Query: 374 LYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           LYVAG+D KIIMKIG   +LGNLIPS++Q++TSG +YAVWE+
Sbjct: 381 LYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSTSGKDYAVWEK 422

BLAST of Lsi04G015650 vs. NCBI nr
Match: XP_038896126.1 (alpha-amylase-like [Benincasa hispida])

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-188
Identity = 313/333 (93.99%), Postives = 324/333 (97.30%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           +ELKSLIA LH+KGIKAIADIVINHRTAEKQD+RGIWCIFEGGTPDSRLDWGPSFICSDD
Sbjct: 91  NELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDSRLDWGPSFICSDD 150

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKE+SEWMRWLKTEIGFDGWRFDFVKGYAPS
Sbjct: 151 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKTEIGFDGWRFDFVKGYAPS 210

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           ITKLYMDQT P+FAVGEKWD LS GQD KPNQNQD+HRDALANWVRASGD VTAFDFTTK
Sbjct: 211 ITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALANWVRASGDIVTAFDFTTK 270

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAV+GELWRLKDSNG PPGFIGIKPESAVTFI+NHDTGSTQRMWPFPSDKVIQGYA
Sbjct: 271 GILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFIENHDTGSTQRMWPFPSDKVIQGYA 330

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTPSIFYDHFVDWGLKEEIMKLS IRARNGIGKTSTVNIMASEGDLYVAGVDGK
Sbjct: 331 YILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRARNGIGKTSTVNIMASEGDLYVAGVDGK 390

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIGANT+LGNLIPSDYQLAT GM+YAVWE+
Sbjct: 391 IIMKIGANTSLGNLIPSDYQLATYGMDYAVWEK 423

BLAST of Lsi04G015650 vs. NCBI nr
Match: XP_022142476.1 (alpha-amylase [Momordica charantia])

HSP 1 Score: 654.1 bits (1686), Expect = 8.3e-184
Identity = 302/333 (90.69%), Postives = 323/333 (97.00%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           +ELKSLIAALH+KGIKAIADIVINHRTAEKQD+RGIWCIFEGGTPD+RLDWGPSFICSDD
Sbjct: 105 NELKSLIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICSDD 164

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           T+YSDGKGNPDSGEPYPPAPDIDHL PQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS
Sbjct: 165 TDYSDGKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 224

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           +TKLYMDQT PDFAVGEKWDSLS+G D K NQNQDAHRD+LANWV+ASGD VTAFDFTTK
Sbjct: 225 VTKLYMDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFTTK 284

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTF+DNHDTGSTQ++WPFPSDKVIQGYA
Sbjct: 285 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQGYA 344

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTP+IFYDHFV+WGLKEEI++LS IRARN IGKTS VNI+AS+ DLYVAGVDGK
Sbjct: 345 YILTHPGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVDGK 404

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIGANTNLGNLIPS++QLATSGMEYAVWE+
Sbjct: 405 IIMKIGANTNLGNLIPSEFQLATSGMEYAVWEK 437

BLAST of Lsi04G015650 vs. NCBI nr
Match: KAG7024254.1 (Alpha-amylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649.4 bits (1674), Expect = 2.1e-182
Identity = 304/366 (83.06%), Postives = 329/366 (89.89%), Query Frame = 0

Query: 50  TTLLLHGNSLFKKIEVDCNETGLQLGIKQQRRMDELKSLIAALHQKGIKAIADIVINHRT 109
           +++  H  S F   +++ ++ G Q         DELKSLIAALH  GIKA+ADIVINHRT
Sbjct: 78  SSVAFHLKSKFVLYDLNASKYGTQ---------DELKSLIAALHNNGIKAVADIVINHRT 137

Query: 110 AEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNP 169
           A+KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNP
Sbjct: 138 ADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYAAAPDIDHLNP 197

Query: 170 QVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQD 229
           QVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMDQT PDFAVGEKWDSLS G D
Sbjct: 198 QVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPD 257

Query: 230 RKPNQNQDAHRDALANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGI 289
            KP+ NQD HR+ALANWVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGI
Sbjct: 258 GKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGI 317

Query: 290 KPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMK 349
           KPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++K
Sbjct: 318 KPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIK 377

Query: 350 LSAIRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGME 409
           LSAIR RNGIG+ STVNIMAS+GDLYVAG+DGKIIMKIG NTNLGNL+PSDY+LA SGM+
Sbjct: 378 LSAIRGRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQ 434

Query: 410 YAVWER 416
           YAVWE+
Sbjct: 438 YAVWEK 434

BLAST of Lsi04G015650 vs. NCBI nr
Match: XP_023536126.1 (alpha-amylase, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649.0 bits (1673), Expect = 2.7e-182
Identity = 301/333 (90.39%), Postives = 316/333 (94.89%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           DELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEGGTPDSRLDWGPSFICSDD
Sbjct: 134 DELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 193

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           TEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP 
Sbjct: 194 TEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPK 253

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALANWVR SGDTVTAFDFTTK
Sbjct: 254 ITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTK 313

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYA
Sbjct: 314 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYA 373

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTPSIFYDHFV+WGLKEE++KLSAIR RNGIG+ STVNIMAS+GDLYVAG+DGK
Sbjct: 374 YILTHPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGK 433

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIG NTNLGNLIPSDY LATSGM+YAVWE+
Sbjct: 434 IIMKIGPNTNLGNLIPSDYVLATSGMQYAVWEK 466

BLAST of Lsi04G015650 vs. NCBI nr
Match: XP_022976296.1 (alpha-amylase [Cucurbita maxima])

HSP 1 Score: 648.3 bits (1671), Expect = 4.6e-182
Identity = 300/333 (90.09%), Postives = 316/333 (94.89%), Query Frame = 0

Query: 83  DELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 142
           DELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEGGTPDSRLDWGPSFICSDD
Sbjct: 92  DELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDD 151

Query: 143 TEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS 202
           TEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP 
Sbjct: 152 TEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPK 211

Query: 203 ITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTK 262
           ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALANWVR SGDTVTAFDFTTK
Sbjct: 212 ITKLYMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTK 271

Query: 263 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 322
           GILQAAVQGELWRLKD NGKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYA
Sbjct: 272 GILQAAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYA 331

Query: 323 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 382
           YILTHPGTPSIFYDHFV+WGLKEE++KLSAIR RNGIG+ STVNIMAS+GDLYVAG+DGK
Sbjct: 332 YILTHPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGK 391

Query: 383 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           IIMKIG NTNLGNLIPSDY+LATSGM+YAVWE+
Sbjct: 392 IIMKIGPNTNLGNLIPSDYELATSGMQYAVWEK 424

BLAST of Lsi04G015650 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 462.2 bits (1188), Expect = 4.3e-130
Identity = 217/333 (65.17%), Postives = 264/333 (79.28%), Query Frame = 0

Query: 84  ELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDT 143
           ELKSLI AL+QKGIKA+ADIVINHRTAE++D++  +C FEGGT D RLDW PSF+C +D 
Sbjct: 94  ELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPSFVCRNDP 153

Query: 144 EYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSI 203
           ++  G GN D+G  +  APDIDHLNP+VQKE+SEWM WLKTEIGF GWRFD+V+GYA SI
Sbjct: 154 KF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVRGYASSI 213

Query: 204 TKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWV-RASGDTVTAFDFTTK 263
           TKLY+  T PDFAVGEKWD +  G D K + +Q+ HR  L  W+  A G  +TAFDFTTK
Sbjct: 214 TKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTK 273

Query: 264 GILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYA 323
           GILQ+AV+GELWRLKDS GKPPG IGI P +AVTFIDNHD   T R W FPSDKV+ GY 
Sbjct: 274 GILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD---TFRTWVFPSDKVLLGYV 333

Query: 324 YILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 383
           YILTHPGTP IFY+H+++WGLKE I KL AIR +NGIG TS+V I A+E DLY+A +D K
Sbjct: 334 YILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLYLAMIDDK 393

Query: 384 IIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 416
           +IMKIG   ++G L+PS++ LA SG+++AVWE+
Sbjct: 394 VIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEK 422

BLAST of Lsi04G015650 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 307.4 bits (786), Expect = 1.8e-83
Identity = 156/335 (46.57%), Postives = 207/335 (61.79%), Query Frame = 0

Query: 82  MDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSD 141
           +DELK  +   H+ GIK + D V+NHR A  +++ G+W +F G     RL+W    + +D
Sbjct: 560 IDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-----RLNWDDRAVVAD 619

Query: 142 DTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAP 201
           D  +  G+GN  SG+ +  AP+IDH    V+K+I EW+ W+  E+G+DGWR DFV+G+  
Sbjct: 620 DPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWG 679

Query: 202 SITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTT 261
              K YMD + P FAVGE WDSLS     + + NQDAHR  + +W+ A+     AFD TT
Sbjct: 680 GYVKDYMDASKPYFAVGEYWDSLSYTYG-EMDYNQDAHRQRIVDWINATSGAAGAFDVTT 739

Query: 262 KGILQAAVQG-ELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQG 321
           KGIL  A+Q  E WRL D  GKPPG +G  P  AVTFI+NHDTGSTQ  W FP  K +QG
Sbjct: 740 KGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQG 799

Query: 322 YAYILTHPGTPSIFYDH-FVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGV 381
           YAYILTHPGTP++F+DH F D+    EI  L ++R R  +   S VNI  SE D+Y A +
Sbjct: 800 YAYILTHPGTPAVFFDHIFSDY--HSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAII 859

Query: 382 DGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWE 415
           D K+ MKIG           ++ +A  G +Y VWE
Sbjct: 860 DEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of Lsi04G015650 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 304.3 bits (778), Expect = 1.5e-82
Identity = 155/335 (46.27%), Postives = 210/335 (62.69%), Query Frame = 0

Query: 85  LKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTE 144
           LKSL+  + Q  ++A+ADIVINHR    +   G++  ++G      L W    +    T 
Sbjct: 92  LKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG----ISLPWDEHAV----TS 151

Query: 145 YSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSIT 204
            + G GN  +G+ +   P++DH    V+K+I  W+RWL+  +GF  +RFDF +GY+ +  
Sbjct: 152 CTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANYV 211

Query: 205 KLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTKGI 264
           K Y+    P F+VGE WDS +       + NQD+HR  + +W+ A+G    AFDFTTKGI
Sbjct: 212 KEYIGAAKPLFSVGECWDSCNY-NGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGI 271

Query: 265 LQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYI 324
           LQ AV+G+ WRL D+ GKPPG +G  P  AVTF+DNHDTGSTQ  WPFPS  V++GYAYI
Sbjct: 272 LQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYI 331

Query: 325 LTHPGTPSIFYDHFVDWG--LKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGK 384
           LTHPG P +FYDHF DWG  + ++I+KL  IR R  I   STV ++ +E +LY A V  K
Sbjct: 332 LTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEK 391

Query: 385 IIMKIGANTNLGNLIPS--DYQLATSGMEYAVWER 416
           I MK+G     G+  PS  D+ LATSG  YAVW +
Sbjct: 392 ICMKLGD----GSWCPSGRDWTLATSGHRYAVWHK 413