Lsi04G015590 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G015590
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Trimethylguanosine synthase
Location	chr04: 23142648 .. 23148082 (-)
RNA-Seq Expression	Lsi04G015590
Synteny	Lsi04G015590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAAACACAAATCCGTTGCACCGCCGAATCAACGTCGCGTTATTTTAGCAAGCTTCGAAAAATGGCGCATAGTTAAATGGCGGTAGAAAGAGTGCCGTCGTAAACCGCTGATCATAGTTCTTGACCGGAGTTTCCTTTTCTACCGACACAATGGGATCTGGCAACGAAGATTCCGAAGACGAAGCCGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGTAACCACCCTTCCTCACCTCCATTCTCGCCGGAAGATTAGGGAAATCGAAACTAGAAGAAAACAGCTGATTTTATTTTAACCGCAGTGTTCTGTTCGACTTCTCTTTGATTTCATTTCACTTGTTAGGGCATTGACGACAATTTCTAATGGTTGCGTTACAGGGACGATGAGACAGAATTAACTAGACGAGTGGAAAGTACTGTAAGTCTCTAATTTCACTGTTTTGCGACATTGATACTTATTGAGTTCTCTTTGCTCCCTGGTTACTTTACAATCTACTAATTTCTTTGTTTGTTTTAACAAGTTAGCTCTTGATGCTGATGATGCCAATAACGAAAAATTCGGGGAGAAAATCTGTACTACTATCAGTGGCAAGTGTTTCATTCGTCAATCTCTTTGTTTGCCTAGCTTTATTTTACTTTTTTCAAGTTATGAGATATTTGTGGCAAAATTTGATGTTCACCCACTATTTCTGTGGCATAAACATGCATGTTGATTGGTGCTAAAGGGTCAGTTGGATTTTTTTTGGCCAGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGGTACTACAATATACTACTTCTGTATATTATTTAGGTTAAATCATGATATTTTTCCTCTAATCAACATGTTGATACTCCTCTACCTCTAGAAGTATGTTTTTAATTAATTAATTTCAACGGTCTTTATCTTCAGTGAATGTTAAAAAACGCTGAACCACATCGCTGAACTATACATCAATGTTGTATCAATTCTACCTTGGTACCACGTTAAGGTATAATTTAACCCATTACATAAATAAGCTATTCCACATTTTTTTCTCCATTCTTTTGGCCATATCCTCATATAGTTATTGAAAATTAACATAGAGTAACTGTCGTGGCTCTATCATATCTTTTGTTTCATTTCTTCTAGAACAATTGCCAGTACTTAGATGAGAGCCGTATATTGGATAGCTAAATGGATTGAAATGTTTTTATGCATTACCATAGCCATTTGGTGGAAATACTTTTTTCTGCATTTATCCATATTTGTGTGATGTTTTGCCATGTAGCAGAAGAGAATTGGAATCACTATGGGTAAAAGAAAAGCAACTGTCAAGCATTTTAGAACCCAGGAAGGACTTCTAGACAAAGAATTGGAGCTTCCCAATGCCAGCAGCAGGGGGGAGATTGAAGCTAACATTAATTTCAATGATGATGCTATAGGTTCTCTATCATACTCGTCTATGGTGAACCAAAGTGAGACATCTGATCATGATGTTGTGTTGGATGCCAATGAATCCCATGTCATCTTTGATCAAAATATTTCACCTAATTCAAGTGGACCTATAAGTGGTGCTGTTGAAGAACAATCTTGCGATGTTATGTGCAATTTTGTGCTAAATAATGGGGGAGACCATGAATTAAGCTTAGGTGATGCTGTGCTGGGGGATCATAGTCATACAAAAGTTAGATTGAGTTCAATTGGTTTAGATAAAGTTCATTCTTCAAGAATGTGTATGACAGGTCTTGACGTCAGCGATAGCAAGCAGGAGGAAGCTGAACCACCTATGGAGTCAGAAGGTTCGTCCACAACTTTACAAGATACTGAAGTGCAGATGACCAACATTGATAGTGCCATTGGACTGCCAGTAGTAGCTGAATCATCTTTCCTTCATACAGAAGCGGACTATAATGAAGATGACCATGTTGTTGGATGTCCTCATGGATCTGGAGAATGGATGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATATGAAGACACATGAGTCTACTTGGAATCCTCCTCCAGGGTTGGAACATTTTGCACTTTCTGATGCCAATTTCACAGAAAATAAACCAATTTCTGAAGTTGTTGAAATGGATGTATTAGAAGACGTAAAATCAGAAGATATTTGTAGTGTGCTTGGTGAAACCAGGTCATGTATGAATTTACTTGGTGATAATGTGCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTGAGAGTAGTGCATCTGTCAATACTTCAGTTAACAGTTATAAGCAATCTGACGAACCTCAAGAGTGGCAGATGAGTTGTAAAAATATTGGGGAAAATATCAGATGCAGGTTCTTGCCTCTTGTCTAGGCTTTCTATTTCTGATTTTCTCTTTTACCTATATGAGTAATTAGTTTGGAGAGGTATATTGTTTACTATTGCAATTTCAGTTGTGAAGGTCATGTCAAACAACTATGTCATGATAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGGAGCTTCTGAACAAAAGTCCTTTGTCCATCGCAAACCTAGTAACATGGACTCACCTGAGATAGGTTGTGTCACCACAGATGATGATGAAGATGCAGTGGGTTTAGCTACTAACAGGTAGCATTTAGAAACAGAATTTTTATTGGCGCCTATTAGCTTTCCTTATCTCACCACAAATTACTCTTTTTGTTTGGGCTCCCTTGCTGTGTTTCCAGTGCTTCTCATATGCTTCAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGGACCTACCATATGTACACTGGGTACTGAGCAAAATCTTTCTGGAAGAAATAGGAAAAAGAAAATGAAAAGAACACGCAGACGTGGACAACTATCTGATAGAAATGAAGGTTTATTTTTTCTGTTTTGCTATGCCTGTCAAATACCCTTTTTTTTAAAAAAAAATTATTTTGAATTTATATTTTTTTATCAATAGACATTTTATTCACGCTCCACTCCATCAAGTTTCATCAATTTGTTGGGTCTGTTATTTATTAAAATATGACACATTTCCTTTGATTGCGACCTTCTTTTCTATGGTCCAATACATTGATTCTTATTCACAATTTATTTTTGTTCTGAAGATTGAAGATCATAAGAACTTGACACTCAAGTTAAAAATTTAGATTATTCCTTAACATATGGGATATAAAATATGTAACTGTGCCATATATAGTCAATTTCAGTTCTATGCAATGCAGATAGGAACAGGAGATCAGAGTTTAGATTACCCTATACATCAAGAATCTGAAATAGAAGTATGAGCTTTTGTCTTAGTAACTTAATGTTGATCTATCTGAGAGGAAACAAATAAATTCATGGGTAAATATTGTGTTTTCCCTTCCTCTTGGAACAAGTTTTCATTTGATGTACTTCTGCGAAAATAGTGGTTTGGTGCTTGCTTCGGGATTATGAACCTTATTTAGAGCTTTAGTAATATTCAACAACATGAGCCTGGCAAATATTGTAGAAGAACAATTTTGGCTGGTGGTGCTTTCTGGTTGTAACTCATAACATAACTGCTACTGCCAAATTACACTCTTTCGGTAGCAGATTAGATTTCTTAGTAAGCCTCATGATAGAAATAGATAATGACATGCCATAAGATTCTTTTCTCCCTGACAGACAAATTTTCTTCTGCAGAATTTCATTCTCCCACAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCGACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAATTGACTCTTTCACTGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGTGTTGTTCGATTTATTTTTTATTCATAAGTTTGGGGGGATAATTGACACTTTAATGCATCTTATAAGCTCAGATAATTGGTTAGGTTGTTGTGTAGTTAATTTACACGTGTATCAGTGGTTGATGAATAGGTGGATCTGATCAGATTCATGTGTCATTCTCTTTTACAACTTCTAAATGATGTTTAATAGAATATACCTGACAACTTCTTTGTTTAATTAACTTGTTAATGATATTACAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCAGCCATATATGGTGTTGAAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCACATCTGAAGGTATTGAGCCTTTTCTTCTAGAACATTTGTGATTTTTCATGTACTGGAATGAGTGTTTGATTTAGAAAACCAATTTCCTGAGATGTGTAATGAAAGCTACCAAGTGTGAGATTTGAAGTGTCTGTTAATTCCAAAAATTCAAAATTCTCCCTTCGAGGACTAAGAACCAGTATCGGCAATGTTTGTACTTTAGATGGTGAAAGTGTCACCTGTATGATCATTGTTAGGGGAAGGTATTACCATAAGAATGCTCATTATGTCTGGGTGAAAGTCACAAGTATTATGATTATCAATTTTTTTTTACTAGTAAGATAATGGATGCAATTATTTGTTGGCATCATTTATTCCTATTTTTCTGATGACAAGTCTGAATATTGTTGCAGCTGTCTTTTGAACTGTGGCTTTGATCTTGCATATTCAATAAAAAAATTTATTTATTTCTTGCAGTTTGGTTTTTATTAAACGTTCAATGCTATTATCTCACTTTTAATTATATTGAAATGTTGTTGGTCTCTACCTGCTACAGGCCGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCGGATTATGCCAGAGTAGATATTTATGACCTACAGACCATGCTCAAACCACATGATGGGTAAGCTTTCTGACTTTGATAGTTCATGCTCAGTTCAGGTTGCAAATAATATTATTCTTACCATCATCTGAGCTTTGCTTTTGCCTGTACCTATGTAGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTAAGAATATTTCATTTTGTGCTCCATATCTGTTGCAAATTATTGGCGGGCTCAAGAATTGACATTTAGATATTATTGAGGCTGGTATGGTGCTTTTTGTGGAAATAAAGCACGGGAGGGGCAGTTGAATTGCCGTCATAATGAATATCTAGGTTTTGCTTTCCATTGTGCAATGTGCAATGTGCAATTTGAAGTCTGCAGGTAGAGCAGTAGTTTCTACTGCAAGTTGA

mRNA sequence

CCAAAACACAAATCCGTTGCACCGCCGAATCAACGTCGCGTTATTTTAGCAAGCTTCGAAAAATGGCGCATAGTTAAATGGCGGTAGAAAGAGTGCCGTCGTAAACCGCTGATCATAGTTCTTGACCGGAGTTTCCTTTTCTACCGACACAATGGGATCTGGCAACGAAGATTCCGAAGACGAAGCCGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGGACGATGAGACAGAATTAACTAGACGAGTGGAAAGTACTGGTCAGTTGGATTTTTTTTGGCCAGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGCAGAAGAGAATTGGAATCACTATGGGTAAAAGAAAAGCAACTGTCAAGCATTTTAGAACCCAGGAAGGACTTCTAGACAAAGAATTGGAGCTTCCCAATGCCAGCAGCAGGGGGGAGATTGAAGCTAACATTAATTTCAATGATGATGCTATAGGTTCTCTATCATACTCGTCTATGGTGAACCAAAGTGAGACATCTGATCATGATGTTGTGTTGGATGCCAATGAATCCCATGTCATCTTTGATCAAAATATTTCACCTAATTCAAGTGGACCTATAAGTGGTGCTGTTGAAGAACAATCTTGCGATGTTATGTGCAATTTTGTGCTAAATAATGGGGGAGACCATGAATTAAGCTTAGGTGATGCTGTGCTGGGGGATCATAGTCATACAAAAGTTAGATTGAGTTCAATTGGTTTAGATAAAGTTCATTCTTCAAGAATGTGTATGACAGGTCTTGACGTCAGCGATAGCAAGCAGGAGGAAGCTGAACCACCTATGGAGTCAGAAGGTTCGTCCACAACTTTACAAGATACTGAAGTGCAGATGACCAACATTGATAGTGCCATTGGACTGCCAGTAGTAGCTGAATCATCTTTCCTTCATACAGAAGCGGACTATAATGAAGATGACCATGTTGTTGGATGTCCTCATGGATCTGGAGAATGGATGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATATGAAGACACATGAGTCTACTTGGAATCCTCCTCCAGGGTTGGAACATTTTGCACTTTCTGATGCCAATTTCACAGAAAATAAACCAATTTCTGAAGTTGTTGAAATGGATGTATTAGAAGACGTAAAATCAGAAGATATTTGTAGTGTGCTTGGTGAAACCAGGTCATGTATGAATTTACTTGGTGATAATGTGCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTGAGAGTAGTGCATCTGTCAATACTTCAGTTAACAGTTATAAGCAATCTGACGAACCTCAAGAGTGGCAGATGAGTTGTAAAAATATTGGGGAAAATATCAGATGCAGTTGTGAAGGTCATGTCAAACAACTATGTCATGATAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGGAGCTTCTGAACAAAAGTCCTTTGTCCATCGCAAACCTAGTAACATGGACTCACCTGAGATAGGTTGTGTCACCACAGATGATGATGAAGATGCAGTGGGTTTAGCTACTAACAGTGCTTCTCATATGCTTCAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGGACCTACCATATGTACACTGGACAAATTTTCTTCTGCAGAATTTCATTCTCCCACAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCGACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAATTGACTCTTTCACTGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCAGCCATATATGGTGTTGAAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCACATCTGAAGGCCGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCGGATTATGCCAGAGTAGATATTTATGACCTACAGACCATGCTCAAACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTTTGCTTTCCATTGTGCAATGTGCAATGTGCAATTTGAAGTCTGCAGGTAGAGCAGTAGTTTCTACTGCAAGTTGA

Coding sequence (CDS)

ATGGGATCTGGCAACGAAGATTCCGAAGACGAAGCCGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGGACGATGAGACAGAATTAACTAGACGAGTGGAAAGTACTGGTCAGTTGGATTTTTTTTGGCCAGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGCAGAAGAGAATTGGAATCACTATGGGTAAAAGAAAAGCAACTGTCAAGCATTTTAGAACCCAGGAAGGACTTCTAGACAAAGAATTGGAGCTTCCCAATGCCAGCAGCAGGGGGGAGATTGAAGCTAACATTAATTTCAATGATGATGCTATAGGTTCTCTATCATACTCGTCTATGGTGAACCAAAGTGAGACATCTGATCATGATGTTGTGTTGGATGCCAATGAATCCCATGTCATCTTTGATCAAAATATTTCACCTAATTCAAGTGGACCTATAAGTGGTGCTGTTGAAGAACAATCTTGCGATGTTATGTGCAATTTTGTGCTAAATAATGGGGGAGACCATGAATTAAGCTTAGGTGATGCTGTGCTGGGGGATCATAGTCATACAAAAGTTAGATTGAGTTCAATTGGTTTAGATAAAGTTCATTCTTCAAGAATGTGTATGACAGGTCTTGACGTCAGCGATAGCAAGCAGGAGGAAGCTGAACCACCTATGGAGTCAGAAGGTTCGTCCACAACTTTACAAGATACTGAAGTGCAGATGACCAACATTGATAGTGCCATTGGACTGCCAGTAGTAGCTGAATCATCTTTCCTTCATACAGAAGCGGACTATAATGAAGATGACCATGTTGTTGGATGTCCTCATGGATCTGGAGAATGGATGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATATGAAGACACATGAGTCTACTTGGAATCCTCCTCCAGGGTTGGAACATTTTGCACTTTCTGATGCCAATTTCACAGAAAATAAACCAATTTCTGAAGTTGTTGAAATGGATGTATTAGAAGACGTAAAATCAGAAGATATTTGTAGTGTGCTTGGTGAAACCAGGTCATGTATGAATTTACTTGGTGATAATGTGCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTGAGAGTAGTGCATCTGTCAATACTTCAGTTAACAGTTATAAGCAATCTGACGAACCTCAAGAGTGGCAGATGAGTTGTAAAAATATTGGGGAAAATATCAGATGCAGTTGTGAAGGTCATGTCAAACAACTATGTCATGATAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGGAGCTTCTGAACAAAAGTCCTTTGTCCATCGCAAACCTAGTAACATGGACTCACCTGAGATAGGTTGTGTCACCACAGATGATGATGAAGATGCAGTGGGTTTAGCTACTAACAGTGCTTCTCATATGCTTCAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGGACCTACCATATGTACACTGGACAAATTTTCTTCTGCAGAATTTCATTCTCCCACAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCGACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAATTGACTCTTTCACTGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCAGCCATATATGGTGTTGAAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCACATCTGAAGGCCGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCGGATTATGCCAGAGTAGATATTTATGACCTACAGACCATGCTCAAACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTTTGCTTTCCATTGTGCAATGTGCAATGTGCAATTTGAAGTCTGCAGGTAGAGCAGTAGTTTCTACTGCAAGTTGA

Protein sequence

MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLDFFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGLLDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHSSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTENKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALLEGSESSASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASEQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICTLDKFSSAEFHSPTITEEYPTSITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVLLSIVQCAMCNLKSAGRAVVSTAS

Homology

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BLAST of Lsi04G015590 vs. ExPASy Swiss-Prot
Match: P85107 (Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 2.0e-58
Identity = 105/176 (59.66%), Postives = 135/176 (76.70%), Query Frame = 0


Query: 542 ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGS----NMIIDSFTGVGG 601
           + KYW QRYRLFSRFDDG+K+DKEGWFSVTPE IA H A R       ++I+D+F GVGG
Sbjct: 638 LAKYWAQRYRLFSRFDDGIKLDKEGWFSVTPEKIAEHIAGRVSQSFNCDIIVDAFCGVGG 697

Query: 602 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLS 661
           N IQF+   K VIAIDIDP KI  A++NA +YGV D+I+FI GDF  LAP LKADV+FLS
Sbjct: 698 NTIQFALTGKRVIAIDIDPVKIDLARNNAEVYGVADKIEFICGDFLLLAPCLKADVVFLS 757

Query: 662 PPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELS 714
           PPWGGPDYA  + +D++TM+ P DG+ +F +++KI   +V FLP+N ++DQ+A L+
Sbjct: 758 PPWGGPDYATAETFDIRTMMSP-DGFEIFRLSQKITNNIVYFLPRNADVDQVASLA 812

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BLAST of Lsi04G015590 vs. ExPASy Swiss-Prot
Match: Q923W1 (Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 2.7e-58
Identity = 103/176 (58.52%), Postives = 135/176 (76.70%), Query Frame = 0


Query: 542 ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGS----NMIIDSFTGVGG 601
           + KYW QRYRLFSRFDDG+K+DKEGWFSVTPE IA H A R       ++++D+F GVGG
Sbjct: 636 LAKYWAQRYRLFSRFDDGIKLDKEGWFSVTPEKIAEHIAGRVSQAFRCDVVVDAFCGVGG 695

Query: 602 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLS 661
           N IQF+   K VIAIDIDP KI  A++NA +YG+ D+I+FI GDF  LAP LKADV+FLS
Sbjct: 696 NTIQFALTGKRVIAIDIDPVKIDLARNNAEVYGIADKIEFICGDFLLLAPCLKADVVFLS 755

Query: 662 PPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELS 714
           PPWGGPDYA  + +D++TM+ P DG+ +F +++KI   +V FLP+N ++DQ+A L+
Sbjct: 756 PPWGGPDYATAETFDIRTMMSP-DGFEIFRLSQKITNNIVYFLPRNADIDQVASLA 810

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BLAST of Lsi04G015590 vs. ExPASy Swiss-Prot
Match: Q96RS0 (Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3)

HSP 1 Score: 224.6 bits (571), Expect = 3.8e-57
Identity = 101/176 (57.39%), Postives = 133/176 (75.57%), Query Frame = 0


Query: 542 ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGS----NMIIDSFTGVGG 601
           + KYW QRYRLFSRFDDG+K+D+EGWFSVTPE IA H A R       ++++D+F GVGG
Sbjct: 644 LAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGG 703

Query: 602 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLS 661
           N IQF+     VIAIDIDP KI  A++NA +YG+ D+I+FI GDF  LA  LKADV+FLS
Sbjct: 704 NTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLS 763

Query: 662 PPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELS 714
           PPWGGPDYA  + +D++TM+ P DG+ +F ++KKI   +V FLP+N ++DQ+A L+
Sbjct: 764 PPWGGPDYATAETFDIRTMMSP-DGFEIFRLSKKITNNIVYFLPRNADIDQVASLA 818

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BLAST of Lsi04G015590 vs. ExPASy Swiss-Prot
Match: Q09814 (Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tgs1 PE=1 SV=3)

HSP 1 Score: 151.8 bits (382), Expect = 3.2e-35
Identity = 84/214 (39.25%), Postives = 121/214 (56.54%), Query Frame = 0


Query: 539 PTSITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPE----PIARHHASRCGSNMIIDSFTG 598
           P ++ KYW  RY LFSRFD+G+ +D + W+SVTPE     IA+         +IID+F+G
Sbjct: 23  PKALKKYWNNRYNLFSRFDEGIWLDYQSWYSVTPEKVAVAIAKSVVDFIQPELIIDAFSG 82

Query: 599 VGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGV-EDQIDFIKGDFFRLAPHLK--- 658
            GGN IQF++    VI+I+IDP KI  A+HN  IYG+   ++ FI+GD       L+   
Sbjct: 83  CGGNTIQFAKYCP-VISIEIDPIKIAMAKHNLEIYGIPSSRVTFIQGDVLDTFKSLQFAK 142

Query: 659 --ADVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQ 718
               ++F+SPPWGGP Y+   +Y L   L P+    LF  A +I+P V  FLP+N ++ +
Sbjct: 143 DYRSLVFMSPPWGGPSYSGKTVYSLND-LNPYAFDVLFKEATRISPYVAAFLPRNTDVKE 202

Query: 719 LAELSLSSDPPWSLEVLLSIVQCAMC---NLKSA 740
           LA      + P+    L      A+C   N+K A
Sbjct: 203 LAAYGSIHNKPYITNFLFEGYAKAICCYFNMKGA 234

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BLAST of Lsi04G015590 vs. ExPASy Swiss-Prot
Match: Q12052 (Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGS1 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 3.4e-29
Identity = 74/178 (41.57%), Postives = 100/178 (56.18%), Query Frame = 0


Query: 544 KYWCQRYRLFSRFDD-GVKMDKEGWFSVTPEPIA---RHHASRCGSN--MIIDSFTGVGG 603
           KYW  R RLFS+ D   + M  E WFSVTPE IA    +    C  N   I+D F G GG
Sbjct: 51  KYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGG 110

Query: 604 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLA-----PHLKAD 663
           N IQF+ +  +V  +D     I     NA  YGV+D+I   +G + +L        +K D
Sbjct: 111 NTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYD 170

Query: 664 VIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLA 711
            +F SPPWGGP+Y R D+YDL+  LKP     +     K++P V+MFLP+N +L+QL+
Sbjct: 171 CVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLS 228

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BLAST of Lsi04G015590 vs. ExPASy TrEMBL
Match: A0A6J1II65 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 583/768 (75.91%), Postives = 624/768 (81.25%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRA+GSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDI+LTEQMNALGLPLSFHTNKEQ+R GITMGKRK TVKH R Q G 
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQSC+V C+ VLNN GDHE   GDA+LGDH+  KVRLS IGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHA--KVRLSPIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE S+LH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASV+TS+NSY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN  S
Sbjct: 421 RASVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H K SNMDSPE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKSSNMDSPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLD-----------------------KFSSAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                           + EFHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEV
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEV 763

[Close]

BLAST of Lsi04G015590 vs. ExPASy TrEMBL
Match: A0A6J1IEG7 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 583/772 (75.52%), Postives = 626/772 (81.09%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRA+GSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDI+LTEQMNALGLPLSFHTNKEQ+R GITMGKRK TVKH R Q G 
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQSC+V C+ VLNN GDHE   GDA+LGDH+  KVRLS IGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHA--KVRLSPIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE S+LH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASV+TS+NSY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN  S
Sbjct: 421 RASVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H K SNMDSPE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKSSNMDSPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLD-----------------------KFSSAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                           + EFHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVLLSI 729
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLE +L +
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLECVLRL 767

[Close]

BLAST of Lsi04G015590 vs. ExPASy TrEMBL
Match: A0A6J1IFL4 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 582/768 (75.78%), Postives = 623/768 (81.12%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRA+GSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDI+LTEQMNALGLPLSFHTNKE +R GITMGKRK TVKH R Q G 
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKE-RRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQSC+V C+ VLNN GDHE   GDA+LGDH+  KVRLS IGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHA--KVRLSPIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE S+LH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASV+TS+NSY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN  S
Sbjct: 421 RASVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H K SNMDSPE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKSSNMDSPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLD-----------------------KFSSAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                           + EFHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEV
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEV 762

[Close]

BLAST of Lsi04G015590 vs. ExPASy TrEMBL
Match: A0A6J1FD09 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 584/768 (76.04%), Postives = 627/768 (81.64%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGSGNE+S  EAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKEQ+R GITMGKR  TVKH R Q+G 
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P +SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GA EEQSCDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK H
Sbjct: 181 ISPNSSGLIHGAFEEQSCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPP GLEHFA  DANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED+K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS++
Sbjct: 361 NESIAEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKN 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNTS+NSY Q DEP EW  + +N  E I CSCEGHVKQ CH+NCSNGFQLIVAN AS
Sbjct: 421 RASVNTSINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM SPE   VT  DDE AV L T+S SH+LQQADHM+GDMHFGN PTIC
Sbjct: 481 EQKTFSHCKPSNMYSPEKAFVTI-DDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTIC 540

Query: 541 TL---------------------DKFS--SAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                      +FS  + EFHSP ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
           TKIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEV
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEV 761

[Close]

BLAST of Lsi04G015590 vs. ExPASy TrEMBL
Match: A0A6J1F792 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 584/772 (75.65%), Postives = 629/772 (81.48%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGSGNE+S  EAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKEQ+R GITMGKR  TVKH R Q+G 
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P +SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GA EEQSCDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK H
Sbjct: 181 ISPNSSGLIHGAFEEQSCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPP GLEHFA  DANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED+K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS++
Sbjct: 361 NESIAEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKN 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNTS+NSY Q DEP EW  + +N  E I CSCEGHVKQ CH+NCSNGFQLIVAN AS
Sbjct: 421 RASVNTSINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM SPE   VT  DDE AV L T+S SH+LQQADHM+GDMHFGN PTIC
Sbjct: 481 EQKTFSHCKPSNMYSPEKAFVTI-DDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTIC 540

Query: 541 TL---------------------DKFS--SAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                      +FS  + EFHSP ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
           TKIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVLLSI 729
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLE +L +
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLECVLRL 765

[Close]

BLAST of Lsi04G015590 vs. NCBI nr
Match: XP_038897816.1 (uncharacterized protein LOC120085727 isoform X1 [Benincasa hispida])

HSP 1 Score: 1217.6 bits (3149), Expect = 0.0e+00
Identity = 624/768 (81.25%), Postives = 651/768 (84.77%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MG  +E+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGCSDEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNENFGE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                  GISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKH RTQ+G 
Sbjct: 61  KICTTISGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHSRTQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E PNASSR EI ANINFNDDAIGSL Y SMVNQSE SD DVVLDANESHVI   N
Sbjct: 121 LDKEVEFPNASSREEIVANINFNDDAIGSLCYLSMVNQSEKSDRDVVLDANESHVISGGN 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPN S  ISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD  HT+VR SSIGL KVH
Sbjct: 181 ISPNLSVLISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD--HTEVRSSSIGLVKVH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S RMCMTGLDV   KQEE EPPME EGSS TLQDTEVQ ++IDS IGLP+V ESS LHTE
Sbjct: 241 SPRMCMTGLDVDHGKQEEVEPPMELEGSSMTLQDTEVQKSDIDSGIGLPIVPESSLLHTE 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNEDDHVVGC   SGEWMVYWDSFY RNYFYNMKTHESTWNPP GLEHFA SDANFTE
Sbjct: 301 ADYNEDDHVVGCIDESGEWMVYWDSFYKRNYFYNMKTHESTWNPPLGLEHFAFSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+P + VV+MDVLED+KSEDIC VL +TRSCMNL+GDNVHCQPPDALL       EGSE 
Sbjct: 361 NEPSAGVVQMDVLEDIKSEDIC-VLDDTRSCMNLIGDNVHCQPPDALLEGSSVVAEGSER 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNT VNSYKQSDEPQE QMSCKNIGENI CSCEGHVKQLCH+NCSNGFQLIVAN AS
Sbjct: 421 VASVNTPVNSYKQSDEPQERQMSCKNIGENIGCSCEGHVKQLCHENCSNGFQLIVANVAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM+SPE+ CVT DDDE AVGLAT+SASHM QQADHMDGDM+FGNGPTIC
Sbjct: 481 EQKTFGHCKPSNMNSPEMDCVTIDDDEGAVGLATSSASHMPQQADHMDGDMYFGNGPTIC 540

Query: 541 TLDKFSSAE-----------------------FHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL    +                         FHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRNRKKKMKRTRRRGQLSNRNEAFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPEPIARHHASRCGSN IIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNTIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAAIYGV DQIDFIKGDFF LAPHLKADVIFLSPPWGGPDYARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAAIYGVGDQIDFIKGDFFCLAPHLKADVIFLSPPWGGPDYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           LKPHDGYFLFNIAKKIAPV+VMFLPKNV+LDQLAELSLSSDPPWSLEV
Sbjct: 721 LKPHDGYFLFNIAKKIAPVIVMFLPKNVSLDQLAELSLSSDPPWSLEV 765

[Close]

BLAST of Lsi04G015590 vs. NCBI nr
Match: XP_038897817.1 (uncharacterized protein LOC120085727 isoform X2 [Benincasa hispida])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 623/768 (81.12%), Postives = 650/768 (84.64%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MG  +E+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGCSDEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNENFGE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                  GISLLPEDIELTEQMNALGLPLSFHTNKE KRIGITMGKRKATVKH RTQ+G 
Sbjct: 61  KICTTISGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMGKRKATVKHSRTQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E PNASSR EI ANINFNDDAIGSL Y SMVNQSE SD DVVLDANESHVI   N
Sbjct: 121 LDKEVEFPNASSREEIVANINFNDDAIGSLCYLSMVNQSEKSDRDVVLDANESHVISGGN 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPN S  ISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD  HT+VR SSIGL KVH
Sbjct: 181 ISPNLSVLISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD--HTEVRSSSIGLVKVH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S RMCMTGLDV   KQEE EPPME EGSS TLQDTEVQ ++IDS IGLP+V ESS LHTE
Sbjct: 241 SPRMCMTGLDVDHGKQEEVEPPMELEGSSMTLQDTEVQKSDIDSGIGLPIVPESSLLHTE 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNEDDHVVGC   SGEWMVYWDSFY RNYFYNMKTHESTWNPP GLEHFA SDANFTE
Sbjct: 301 ADYNEDDHVVGCIDESGEWMVYWDSFYKRNYFYNMKTHESTWNPPLGLEHFAFSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+P + VV+MDVLED+KSEDIC VL +TRSCMNL+GDNVHCQPPDALL       EGSE 
Sbjct: 361 NEPSAGVVQMDVLEDIKSEDIC-VLDDTRSCMNLIGDNVHCQPPDALLEGSSVVAEGSER 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNT VNSYKQSDEPQE QMSCKNIGENI CSCEGHVKQLCH+NCSNGFQLIVAN AS
Sbjct: 421 VASVNTPVNSYKQSDEPQERQMSCKNIGENIGCSCEGHVKQLCHENCSNGFQLIVANVAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM+SPE+ CVT DDDE AVGLAT+SASHM QQADHMDGDM+FGNGPTIC
Sbjct: 481 EQKTFGHCKPSNMNSPEMDCVTIDDDEGAVGLATSSASHMPQQADHMDGDMYFGNGPTIC 540

Query: 541 TLDKFSSAE-----------------------FHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL    +                         FHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRNRKKKMKRTRRRGQLSNRNEAFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPEPIARHHASRCGSN IIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNTIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAAIYGV DQIDFIKGDFF LAPHLKADVIFLSPPWGGPDYARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAAIYGVGDQIDFIKGDFFCLAPHLKADVIFLSPPWGGPDYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           LKPHDGYFLFNIAKKIAPV+VMFLPKNV+LDQLAELSLSSDPPWSLEV
Sbjct: 721 LKPHDGYFLFNIAKKIAPVIVMFLPKNVSLDQLAELSLSSDPPWSLEV 764

[Close]

BLAST of Lsi04G015590 vs. NCBI nr
Match: XP_023535334.1 (uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535335.1 uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535336.1 uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 589/768 (76.69%), Postives = 627/768 (81.64%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKEQ+R GITMGKRK TVKH R Q+G 
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQ CDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA  DANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNTS++SY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN AS
Sbjct: 421 RASVNTSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM +PE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKPSNMYAPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLD-----------------------KFSSAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                           + EFHSP ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
           TKIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEV
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEV 763

[Close]

BLAST of Lsi04G015590 vs. NCBI nr
Match: XP_023535337.1 (uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 588/768 (76.56%), Postives = 626/768 (81.51%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKE +R GITMGKRK TVKH R Q+G 
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRKTTVKHSRIQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQ CDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA  DANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNTS++SY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN AS
Sbjct: 421 RASVNTSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM +PE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKPSNMYAPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLD-----------------------KFSSAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                           + EFHSP ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
           TKIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEV
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEV 762

[Close]

BLAST of Lsi04G015590 vs. NCBI nr
Match: XP_022975951.1 (uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975952.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975953.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975954.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975955.1 uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 583/768 (75.91%), Postives = 624/768 (81.25%), Query Frame = 0


Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRA+GSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDI+LTEQMNALGLPLSFHTNKEQ+R GITMGKRK TVKH R Q G 
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQSC+V C+ VLNN GDHE   GDA+LGDH+  KVRLS IGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHA--KVRLSPIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE S+LH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASV+TS+NSY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN  S
Sbjct: 421 RASVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H K SNMDSPE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKSSNMDSPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLD-----------------------KFSSAEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TL                           + EFHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEV 725
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEV
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEV 763

[Close]

BLAST of Lsi04G015590 vs. TAIR 10
Match: AT1G45231.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 1.8e-86
Identity = 193/501 (38.52%), Postives = 267/501 (53.29%), Query Frame = 0


Query: 235 LDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEADYNEDDH 294
           L++  +  EE E P   E     +Q  EV+  N +  +G      S FL       + D 
Sbjct: 108 LNLVSALNEEVESPCFEE---DCVQVIEVEEENHEVVVG------SCFLGN----GDGDS 167

Query: 295 VVGC----PHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTENKPI 354
           V+       H S  W VYWDSFY R+YFYN KT ES W PP G+EH A SD    E+  +
Sbjct: 168 VLASETIDSHDSSVWKVYWDSFYGRSYFYNFKTQESKWEPPLGMEHLAYSD----ESHNL 227

Query: 355 SEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDAL-------LEGSESSASV 414
           SE+V     +D+                 +LGD+V     D L       LE +E++  V
Sbjct: 228 SELVIEKHHDDLSG--------------TVLGDDVPFDKADDLGGVCQSQLE-AEATEEV 287

Query: 415 NTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASEQKS 474
           N+ +++Y+++    +  +   ++GE                    G  ++ +   ++++S
Sbjct: 288 NSLIDTYQETSIGNQ-SLDITSLGEE-----------------GTGAYVVSSVRKAKKES 347

Query: 475 FVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICTLDK 534
              R    +                                                L+ 
Sbjct: 348 RRSRAKKKL------------------------------------------------LNS 407

Query: 535 FSSAEFHSPTITEEYPTSITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRC 594
           ++  E     + EEY   + KYWCQRY LFSRFD+G+KMD+EGWFSVTPE IA+HHA+RC
Sbjct: 408 YTGTEMKG--VPEEYSPILGKYWCQRYLLFSRFDEGIKMDEEGWFSVTPELIAKHHATRC 467

Query: 595 GSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFF 654
              ++ID FTGVGGNAIQF+ R+ +VIAID+DP K+  A+HNAAIYGV D+IDF+KGDFF
Sbjct: 468 NEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKKLDLAKHNAAIYGVADKIDFVKGDFF 508

Query: 655 RLAPHLKADVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKN 714
            LA +LKA  +FLSPPWGGPDY +   YD++TML+P DG  LF  A  IA  ++MFLP+N
Sbjct: 528 DLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLRPRDGDALFKAAMNIASTIIMFLPRN 508

Query: 715 VNLDQLAELSLSSDPPWSLEV 725
           V+++QLAEL+LS+ PPWSLEV
Sbjct: 588 VDINQLAELALSTSPPWSLEV 508

[Close]

BLAST of Lsi04G015590 vs. TAIR 10
Match: AT1G30550.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 3.4e-61
Identity = 109/193 (56.48%), Postives = 142/193 (73.58%), Query Frame = 0


Query: 534 ITEEYPTS--ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDS 593
           I +E+ T+  I++YW QRY LFS++D G++MD+EGW+SVTPE IA   A RC   ++ID 
Sbjct: 9   IEKEHGTNPKISRYWIQRYDLFSKYDQGIEMDEEGWYSVTPEEIAIKQAERCRGKVVIDC 68

Query: 594 FTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKA 653
           F+GVGGN IQF++    VIAIDIDP KI  A +NA +YGV ++IDF+ GDF +LAP LK 
Sbjct: 69  FSGVGGNTIQFAKVCSSVIAIDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQLAPSLKG 128

Query: 654 DVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE 713
           DV+FLSPPWGGP Y++V+ Y L  ML P DGY LF  A  I P ++MFLPKN++L QL E
Sbjct: 129 DVLFLSPPWGGPTYSKVESYKLD-MLLPRDGYSLFQTALSITPNIIMFLPKNIDLAQLEE 188

Query: 714 LSLSSDPPWSLEV 725
           L+  S PP +LE+
Sbjct: 189 LACLSSPPLTLEI 200

[Close]

BLAST of Lsi04G015590 vs. TAIR 10
Match: AT1G30550.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 229.9 bits (585), Expect = 6.5e-60
Identity = 104/183 (56.83%), Postives = 136/183 (74.32%), Query Frame = 0


Query: 542 ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQ 601
           ITKYW QRY LFSR+D G++MD+EGW+SVTPE IA   A R    ++ID F+GVGGN IQ
Sbjct: 253 ITKYWIQRYDLFSRYDQGIEMDEEGWYSVTPEEIAIKQAQRYRGKVVIDCFSGVGGNTIQ 312

Query: 602 FSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWG 661
           F++    V+AIDIDP K+  A +NA +YGV +++DF+ GDF +LAP LK DV+FLSPPWG
Sbjct: 313 FAKVCSSVVAIDIDPVKVELAMNNAMVYGVANRVDFVIGDFIQLAPSLKGDVVFLSPPWG 372

Query: 662 GPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWS 721
           GP Y   + Y+L  ML+P DGY LF IA+ I P ++MFLP+NV+L Q+ EL+  S PP +
Sbjct: 373 GPMYRDFESYNLD-MLQPRDGYSLFQIAQSITPNIIMFLPRNVDLAQVEELAWLSSPPLN 432

Query: 722 LEV 725
           LE+
Sbjct: 433 LEI 434

[Close]

BLAST of Lsi04G015590 vs. TAIR 10
Match: AT3G21300.1 (RNA methyltransferase family protein )

HSP 1 Score: 48.9 bits (115), Expect = 2.1e-05
Identity = 26/80 (32.50%), Postives = 41/80 (51.25%), Query Frame = 0


Query: 584 GSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFF 643
           GS +++D F G G   +  ++RAKHV   ++ P  I  A  NA I G+E+   FI+GD  
Sbjct: 399 GSEVVLDLFCGTGTIGLTLARRAKHVYGYEVVPQAITDAHKNAQINGIEN-ATFIQGDLN 458

Query: 644 RLAPHL-----KADVIFLSP 659
           ++         K D++   P
Sbjct: 459 KIGEDFGNNFPKPDIVISDP 477

The following BLAST results are available for this feature:

BLAST of Lsi04G015590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P85107	2.0e-58	59.66	Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1	[more]
Q923W1	2.7e-58	58.52	Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2	[more]
Q96RS0	3.8e-57	57.39	Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3	[more]
Q09814	3.2e-35	39.25	Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484...	[more]
Q12052	3.4e-29	41.57	Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2...	[more]

BLAST of Lsi04G015590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1II65	0.0e+00	75.91	Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=...	[more]
A0A6J1IEG7	0.0e+00	75.52	Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=...	[more]
A0A6J1IFL4	0.0e+00	75.78	Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=...	[more]
A0A6J1FD09	0.0e+00	76.04	Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S...	[more]
A0A6J1F792	0.0e+00	75.65	Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S...	[more]

BLAST of Lsi04G015590 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897816.1	0.0e+00	81.25	uncharacterized protein LOC120085727 isoform X1 [Benincasa hispida]	[more]
XP_038897817.1	0.0e+00	81.12	uncharacterized protein LOC120085727 isoform X2 [Benincasa hispida]	[more]
XP_023535334.1	0.0e+00	76.69	uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_023535337.1	0.0e+00	76.56	uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022975951.1	0.0e+00	75.91	uncharacterized protein LOC111476503 isoform X1 [Cucurbita maxima] >XP_022975952...	[more]

BLAST of Lsi04G015590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45231.2	1.8e-86	38.52	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G30550.2	3.4e-61	56.48	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G30550.1	6.5e-60	56.83	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G21300.1	2.1e-05	32.50	RNA methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019012	RNA cap guanine-N2 methyltransferase	PFAM	PF09445	Methyltransf_15	coord: 586..713 e-value: 3.8E-39 score: 133.9
None	No IPR available	GENE3D	2.20.70.10		coord: 291..349 e-value: 1.2E-6 score: 29.9
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 535..739 e-value: 2.7E-73 score: 248.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 237..257
None	No IPR available	PANTHER	PTHR14741	S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED	coord: 523..724
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 588..686 e-value: 6.78246E-10 score: 55.1286
IPR001202	WW domain	PROSITE	PS01159	WW_DOMAIN_1	coord: 305..331
IPR001202	WW domain	PROSITE	PS50020	WW_DOMAIN_2	coord: 305..333 score: 10.521601
IPR001202	WW domain	CDD	cd00201	WW	coord: 305..333 e-value: 0.00253499 score: 34.0406
IPR036020	WW domain superfamily	SUPERFAMILY	51045	WW domain	coord: 297..332
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 548..746

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G015590.1	Lsi04G015590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009452	7-methylguanosine RNA capping
biological_process	GO:0001510	RNA methylation
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0005515	protein binding