Lsi04G014570 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G014570
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRNA-binding protein 1-like
Locationchr04: 22334896 .. 22335627 (-)
RNA-Seq ExpressionLsi04G014570
SyntenyLsi04G014570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATGAGTAATATTAGGATGGAAAACAACAACGATCAGTGTAGGGGCAGAAAGATTTTTGTTGGTGGCTTATCGGCTGAATTGACTGAGGATGAGTTTAAAAATTACTTTGAAAAGTTTGGTAAGATAACCGATGTAGTTGTGATGCATGATAGTGTAACCAACCGCCCTAGAGGATTTGGATTCATCACATTTGATTCTGTAGAATCTGTTGACTCTGTTCTGCAGAATAACTTTCATGAGCTGAATGGTAGGCGTGTGGAGGTGAAGAGAGCTATCCCAAAAAAGGGAAACTACAATGTTGGTCATATTCCCAATGCTAGAACACAGGGAGGAAAAAGCCCTCTTCCTAATAGGAATGTTCCTTTCCCTTACCCGTATTATGGTTCTGGTTATACAATTTATCCTGGTTTTACACCTTTTCCTCATTATGGAGGACCTGGAGTTGATTTGTATGGTTCAAACTTTTATGGGAATTGGTTTCCCCCTAGCTTTGGTAACATGGCTTCGGACGGCGCATGGTATGCCCCAAATACTTTTGGTGTTGGGATGTTTCCTCCACCTTATAGAAATTCTCTGATCCCTCCATCATACTCAAGCAAGGGGATCTCAGATACAGGGACTGGAAATGGAATGGCAATAAGAAACGGGGAGATAAAGCAGAACACTTCTGGAAAACGAAGTATGTCAGCTGATGCACTGCCATTTCAGATCAAAGACAGAACTTGA

mRNA sequence

ATGAATATGAGTAATATTAGGATGGAAAACAACAACGATCAGTGTAGGGGCAGAAAGATTTTTGTTGGTGGCTTATCGGCTGAATTGACTGAGGATGAGTTTAAAAATTACTTTGAAAAGTTTGGTAAGATAACCGATGTAGTTGTGATGCATGATAGTGTAACCAACCGCCCTAGAGGATTTGGATTCATCACATTTGATTCTGTAGAATCTGTTGACTCTGTTCTGCAGAATAACTTTCATGAGCTGAATGGTAGGCGTGTGGAGGTGAAGAGAGCTATCCCAAAAAAGGGAAACTACAATGTTGGTCATATTCCCAATGCTAGAACACAGGGAGGAAAAAGCCCTCTTCCTAATAGGAATGTTCCTTTCCCTTACCCGTATTATGGTTCTGGTTATACAATTTATCCTGGTTTTACACCTTTTCCTCATTATGGAGGACCTGGAGTTGATTTGTATGGTTCAAACTTTTATGGGAATTGGTTTCCCCCTAGCTTTGGTAACATGGCTTCGGACGGCGCATGGTATGCCCCAAATACTTTTGGTGTTGGGATGTTTCCTCCACCTTATAGAAATTCTCTGATCCCTCCATCATACTCAAGCAAGGGGATCTCAGATACAGGGACTGGAAATGGAATGGCAATAAGAAACGGGGAGATAAAGCAGAACACTTCTGGAAAACGAAGTATGTCAGCTGATGCACTGCCATTTCAGATCAAAGACAGAACTTGA

Coding sequence (CDS)

ATGAATATGAGTAATATTAGGATGGAAAACAACAACGATCAGTGTAGGGGCAGAAAGATTTTTGTTGGTGGCTTATCGGCTGAATTGACTGAGGATGAGTTTAAAAATTACTTTGAAAAGTTTGGTAAGATAACCGATGTAGTTGTGATGCATGATAGTGTAACCAACCGCCCTAGAGGATTTGGATTCATCACATTTGATTCTGTAGAATCTGTTGACTCTGTTCTGCAGAATAACTTTCATGAGCTGAATGGTAGGCGTGTGGAGGTGAAGAGAGCTATCCCAAAAAAGGGAAACTACAATGTTGGTCATATTCCCAATGCTAGAACACAGGGAGGAAAAAGCCCTCTTCCTAATAGGAATGTTCCTTTCCCTTACCCGTATTATGGTTCTGGTTATACAATTTATCCTGGTTTTACACCTTTTCCTCATTATGGAGGACCTGGAGTTGATTTGTATGGTTCAAACTTTTATGGGAATTGGTTTCCCCCTAGCTTTGGTAACATGGCTTCGGACGGCGCATGGTATGCCCCAAATACTTTTGGTGTTGGGATGTTTCCTCCACCTTATAGAAATTCTCTGATCCCTCCATCATACTCAAGCAAGGGGATCTCAGATACAGGGACTGGAAATGGAATGGCAATAAGAAACGGGGAGATAAAGCAGAACACTTCTGGAAAACGAAGTATGTCAGCTGATGCACTGCCATTTCAGATCAAAGACAGAACTTGA

Protein sequence

MNMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNTFGVGMFPPPYRNSLIPPSYSSKGISDTGTGNGMAIRNGEIKQNTSGKRSMSADALPFQIKDRT
Homology
BLAST of Lsi04G014570 vs. ExPASy Swiss-Prot
Match: Q9C652 (RNA-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBP1 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.0e-32
Identity = 75/145 (51.72%), Postives = 101/145 (69.66%), Query Frame = 0

Query: 1   MNMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRG 60
           MN     M +N    R +KIFVGGLS+  TE+EFK+YFE+FG+ TDVVVMHD VTNRPRG
Sbjct: 103 MNGGLREMSSNGVTSRTKKIFVGGLSSNTTEEEFKSYFERFGRTTDVVVMHDGVTNRPRG 162

Query: 61  FGFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKG----NYNVGHIPNARTQGGKSP 120
           FGF+T+DS +SV+ V+Q+NFHEL+ +RVEVKRAIPK+G    N N  +IP + +    +P
Sbjct: 163 FGFVTYDSEDSVEVVMQSNFHELSDKRVEVKRAIPKEGIQSNNGNAVNIPPSYSSFQATP 222

Query: 121 -LPNRN-----VPFPYPYYGSGYTI 136
            +P +N     + FP P +G  + +
Sbjct: 223 YVPEQNGYGMVLQFPPPVFGYHHNV 247

BLAST of Lsi04G014570 vs. ExPASy Swiss-Prot
Match: Q8W034 (Heterogeneous nuclear ribonucleoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=RNP1 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 7.4e-26
Identity = 90/259 (34.75%), Postives = 124/259 (47.88%), Query Frame = 0

Query: 2   NMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGF 61
           N++  R    +   + +KIFVGGL   LT++EF+ YFE +G +TDV +M+D  TNRPRGF
Sbjct: 94  NLNTSRSSGGDAYNKTKKIFVGGLPPTLTDEEFRQYFEVYGPVTDVAIMYDQATNRPRGF 153

Query: 62  GFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSP----- 121
           GF++FDS ++VDSVL   FH+L+G++VEVKRA+PK  N   G     R+ GG        
Sbjct: 154 GFVSFDSEDAVDSVLHKTFHDLSGKQVEVKRALPKDANPGGG----GRSMGGGGSGGYQG 213

Query: 122 -----------------LPNRNVPFPYPYYGS--------------GYTIYPGFT-PFPH 181
                            L +++V    P YGS              GY  Y G+T     
Sbjct: 214 YGGNESSYDGRMDSNRFLQHQSVGNGLPSYGSSGYGAGYGNGSNGAGYGAYGGYTGSAGG 273

Query: 182 YGGPGVDLYG-SNFYGNWFPPSFGNMASDGAWYAPNTFGVGMFPPPYRN----------S 213
           YG      YG +N  G  +  S G +A   +W  P + G G   P Y +           
Sbjct: 274 YGAGATAGYGATNIPGAGYGSSTG-VAPRNSWDTPASSGYG--NPGYGSGAAHSGYGVPG 333

BLAST of Lsi04G014570 vs. ExPASy Swiss-Prot
Match: Q96EP5 (DAZ-associated protein 1 OS=Homo sapiens OX=9606 GN=DAZAP1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-19
Identity = 66/185 (35.68%), Postives = 93/185 (50.27%), Query Frame = 0

Query: 12  NDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDSVES 71
           +D  +  KIFVGG+     E E + YF+KFG +T+VV+++D+   RPRGFGFITF+  +S
Sbjct: 107 SDNSKSNKIFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQS 166

Query: 72  VDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPYYGS 131
           VD  +  +FH++ G++VEVKRA P+         P A +Q G   +PN           +
Sbjct: 167 VDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGA-SQWGSRVVPNA---------AN 226

Query: 132 GYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNTF-------GVG 190
           G+   P  T    YG  G+ +      G + PP  G     GA   P  F         G
Sbjct: 227 GWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGR----GAPPPPPPFTSYIVSTPPG 277

BLAST of Lsi04G014570 vs. ExPASy Swiss-Prot
Match: Q98SJ2 (DAZ-associated protein 1 OS=Xenopus laevis OX=8355 GN=dazap1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-19
Identity = 66/205 (32.20%), Postives = 93/205 (45.37%), Query Frame = 0

Query: 9   ENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDS 68
           E   +  R  KIFVGG+     E E K YF +FG +T+VV+++D+   RPRGFGFITF+ 
Sbjct: 105 EPRTENSRSNKIFVGGIPHNCGETELKEYFNRFGVVTEVVMIYDAEKQRPRGFGFITFED 164

Query: 69  VESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPY 128
            +SVD  +  +FH++ G++VEVKRA P+         P +   G ++     N       
Sbjct: 165 EQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQTPGPPGSNQWGSRAMQSTAN------- 224

Query: 129 YGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPP-----SFGNMASDGAWYAPNTFG- 188
              G+T           G P     G +  G W P       +G  A  G    P +F  
Sbjct: 225 ---GWT-----------GQPPQTWQGYSPQGMWMPTGQTIGGYGQPAGRGGPPPPPSFAP 284

Query: 189 ------VGMFPPPYRNSLIPPSYSS 202
                  G FPPP      PP Y++
Sbjct: 285 FLVSTTPGPFPPPQG---FPPGYAT 285

BLAST of Lsi04G014570 vs. ExPASy Swiss-Prot
Match: Q920Q6 (RNA-binding protein Musashi homolog 2 OS=Mus musculus OX=10090 GN=Msi2 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-19
Identity = 59/142 (41.55%), Postives = 81/142 (57.04%), Query Frame = 0

Query: 16  RGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDSVESVDSV 75
           R +KIFVGGLSA    ++ K YFE+FGK+ D ++M D  TNR RGFGF+TF++ + V+ V
Sbjct: 108 RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKV 167

Query: 76  LQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPF-------PYPY 135
            + +FHE+N + VE K+A PK+  +  G      T+G    LP     F        YP 
Sbjct: 168 CEIHFHEINNKMVECKKAQPKEVMFPPG------TRGRARGLPYTMDAFMLGMGMLGYPN 227

Query: 136 YGSGY-TIYPGFTPFPHYGGPG 150
           + + Y   YPGF P   Y  PG
Sbjct: 228 FVATYGRGYPGFAPSYGYQFPG 243

BLAST of Lsi04G014570 vs. ExPASy TrEMBL
Match: A0A0A0L563 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G644730 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 8.3e-97
Identity = 190/244 (77.87%), Postives = 203/244 (83.20%), Query Frame = 0

Query: 3   MSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFG 62
           MS  RM+NNNDQCRGRKIFVGGLSAELTE+EF++YFE FG+ITDVVVMHDSVTNRPRGFG
Sbjct: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60

Query: 63  FITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPL--PNR 122
           F+TF+S+ESVDSVLQ NFHELNGRRVEVKRAIPKKGNYN  HIPNAR    KSPL   NR
Sbjct: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNAR--AAKSPLLNGNR 120

Query: 123 NVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNT 182
           NVPFPYPYYGSGY IYPGFT FPHYG   VD YGSNFYGNWFPP   N+ASDG W+ PN 
Sbjct: 121 NVPFPYPYYGSGYPIYPGFTHFPHYGA-AVDSYGSNFYGNWFPPGSANIASDGWWHGPNF 180

Query: 183 FGVGMFPPPYRNSLIPPSYSSKGISDTGTGNGMA-IRNGEIKQNTSGKRSMSADALPFQI 242
           F VGM PP  RNSL+ P   SKGI   G+ NGMA  RNGEIKQNT+GKR MSADA PFQI
Sbjct: 181 FSVGMLPPLCRNSLMHP---SKGIY-IGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQI 237

Query: 243 KDRT 244
           KDRT
Sbjct: 241 KDRT 237

BLAST of Lsi04G014570 vs. ExPASy TrEMBL
Match: A0A6J1J2K5 (RNA-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481242 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 9.9e-82
Identity = 166/241 (68.88%), Postives = 184/241 (76.35%), Query Frame = 0

Query: 3   MSN-IRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGF 62
           MSN  RME   DQ RGRKIFVGGLSAELTED+FKNYFEKFG++TDVVVM DSVT RPRGF
Sbjct: 1   MSNGTRME---DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGF 60

Query: 63  GFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRN 122
           GFITFDSV+SVDSVLQNNFHELNGRRVEVKRAIPKKGNYN GHI        KSPL   N
Sbjct: 61  GFITFDSVQSVDSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-------SKSPLSESN 120

Query: 123 VPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNTF 182
           V   YPYY  GYT+YPGFTPFP Y GPG  LYGS+ YGNWF PSFG MAS  AWY PN F
Sbjct: 121 VLPSYPYYSCGYTVYPGFTPFPPY-GPGFGLYGSDPYGNWFSPSFGPMASPAAWYGPNFF 180

Query: 183 GVGMFPPPYRNSLIPPSYSSKGISDTGTGNGMAIRNGEIKQNTSGKRSMSADALPFQIKD 242
             GMFPPP++NSL+ P+YSS G+S    G   A    +++  T  +++MSADA+PF  KD
Sbjct: 181 --GMFPPPHKNSLVYPTYSSNGLSTPVAGYETADARRKVEPPTRAQKNMSADAVPFHAKD 228

BLAST of Lsi04G014570 vs. ExPASy TrEMBL
Match: A0A6J1FS87 (RNA-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446397 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.2e-81
Identity = 160/230 (69.57%), Postives = 178/230 (77.39%), Query Frame = 0

Query: 13  DQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDSVESV 72
           DQ RGRKIFVGGLSAELTED+FKNYFEKFG++TDVVVM DSVT RPRGFGFITFDSV+SV
Sbjct: 9   DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGFGFITFDSVQSV 68

Query: 73  DSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPYYGSG 132
           DSVLQNNFHELNGRRVEVKRAIPKKGNYN GHI        KSPL   NV   YPYY  G
Sbjct: 69  DSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-------SKSPLSESNVLPSYPYYSCG 128

Query: 133 YTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNTFGVGMFPPPYRN 192
           YT+YPGFTPFP Y GPG  LYGS+ YGNWF PSFG MAS  AWYAPN F  GMFPPP++N
Sbjct: 129 YTVYPGFTPFPPY-GPGFGLYGSDPYGNWFSPSFGPMASPAAWYAPNFF--GMFPPPHKN 188

Query: 193 SLIPPSYSSKGISDTGTGNGMAIRNGEIKQNTSGKRSMSADALPFQIKDR 243
           SL+ P+YSS G+S    G   A    ++   T  +++MSADA+PF  KD+
Sbjct: 189 SLVYPTYSSNGLSTPVAGYETADARRKVDPPTRAQKNMSADAVPFHAKDK 228

BLAST of Lsi04G014570 vs. ExPASy TrEMBL
Match: A0A6J1C724 (RNA-binding protein 1-like OS=Momordica charantia OX=3673 GN=LOC111008498 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 2.0e-66
Identity = 148/251 (58.96%), Postives = 177/251 (70.52%), Query Frame = 0

Query: 1   MNMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRG 60
           MN S+ RME+NNDQ RG+KIFVGGL ++LTE+EFK+YFEKFG+ITDVVVM DSVTNRPRG
Sbjct: 1   MNKSSNRMESNNDQSRGKKIFVGGLPSDLTENEFKSYFEKFGQITDVVVMQDSVTNRPRG 60

Query: 61  FGFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNR 120
           FGFITFDSVESV+ VLQNNFHELNGRRVEVKRA+PK  N N     ++  Q GKSPL + 
Sbjct: 61  FGFITFDSVESVNYVLQNNFHELNGRRVEVKRAVPKNRNCN-----SSNPQRGKSPL-SP 120

Query: 121 NVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNW-FPPSFGN------MASDG 180
           +VP  YP++ SG  +YPG+ PFPHY       YGS+ YG+W +   +G       +    
Sbjct: 121 HVPLYYPHFSSGPVVYPGYAPFPHY-------YGSSLYGSWHYNGLYGTGHEAPPLPLGS 180

Query: 181 AWYAPNTFGVGMFPPPYRNSLIPPSYSSKG--ISDTGTGNGMAIRNGEIKQNTSGKRSMS 240
           AWYAPN FG GMFP PY NS+I P YSSKG  + D   GNG   RNG          +MS
Sbjct: 181 AWYAPNVFGDGMFPSPYINSVIHPVYSSKGGRLLDPMAGNGR--RNG----------NMS 226

Query: 241 ADALPFQIKDR 243
           ADA+PFQ+ +R
Sbjct: 241 ADAVPFQLNNR 226

BLAST of Lsi04G014570 vs. ExPASy TrEMBL
Match: A0A5N6R9D7 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_013735 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 5.5e-48
Identity = 105/212 (49.53%), Postives = 134/212 (63.21%), Query Frame = 0

Query: 9   ENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDS 68
           +N ++Q R +KIFVGGLSA LTE+EFKNYF++FG++TDVVVMHDS T+RPRGFGFITFDS
Sbjct: 108 DNGDNQFRTKKIFVGGLSASLTEEEFKNYFKRFGRVTDVVVMHDSTTHRPRGFGFITFDS 167

Query: 69  VESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPY 128
            ESV++V+QN+FHELNGR VEVKRA+PK+ N       N R +GG      R  P  +P 
Sbjct: 168 EESVENVMQNSFHELNGRLVEVKRAVPKEVNNGSDSGFNTRIEGG------RGSPGSFPP 227

Query: 129 YGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPS--------FGNMASDGAWYAPNT 188
           Y   Y I PG+ PFP + G G  LYG++ Y  W+P             +A    WY P  
Sbjct: 228 YSLRYWILPGYVPFPGFSGGGGSLYGASMYSGWYPSGGSGGVGSVVAPLAPRNPWYGPRV 287

Query: 189 FGVGMFPPPYRNSLIPPSYSSKGISDTGTGNG 213
            G G FP P  ++ I P+Y + G+   G   G
Sbjct: 288 IGAGAFPLPPGSASIYPAYFTGGVGGIGMSAG 313

BLAST of Lsi04G014570 vs. NCBI nr
Match: XP_038898856.1 (RNA-binding protein 1-like [Benincasa hispida])

HSP 1 Score: 453.8 bits (1166), Expect = 9.7e-124
Identity = 220/247 (89.07%), Postives = 229/247 (92.71%), Query Frame = 0

Query: 1   MNMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRG 60
           M MS  RMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRG
Sbjct: 1   MIMSTTRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRG 60

Query: 61  FGFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLP-- 120
           FGFITF+SVESVDSVLQ+NFHELNGRRVEVKRAIPKKGNYN GHIPNARTQGGKSPLP  
Sbjct: 61  FGFITFESVESVDSVLQSNFHELNGRRVEVKRAIPKKGNYNGGHIPNARTQGGKSPLPNS 120

Query: 121 NRNVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAP 180
           NRNVPFPYPYYGS Y +YPGFTPFPHYGGPGVDLYGS+ YGNWFPPSFGNMAS+G WY P
Sbjct: 121 NRNVPFPYPYYGSSYPVYPGFTPFPHYGGPGVDLYGSHLYGNWFPPSFGNMASEGTWYGP 180

Query: 181 NTFGVGMFPPPYRNSLIPPSYSSKGISDTGTGNGM--AIRNGEIKQNTSGKRSMSADALP 240
           N FGVG+FPPPYRNSLIPP YSSKG+SDTGTGNGM    RNGEIKQNT+GKR MSADA+P
Sbjct: 181 NFFGVGIFPPPYRNSLIPPIYSSKGLSDTGTGNGMVDTRRNGEIKQNTTGKRIMSADAVP 240

Query: 241 FQIKDRT 244
           FQIKDRT
Sbjct: 241 FQIKDRT 247

BLAST of Lsi04G014570 vs. NCBI nr
Match: KAE8649895.1 (hypothetical protein Csa_012205 [Cucumis sativus])

HSP 1 Score: 365.9 bits (938), Expect = 2.6e-97
Identity = 191/246 (77.64%), Postives = 205/246 (83.33%), Query Frame = 0

Query: 1   MNMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRG 60
           +NMS  RM+NNNDQCRGRKIFVGGLSAELTE+EF++YFE FG+ITDVVVMHDSVTNRPRG
Sbjct: 51  LNMSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRG 110

Query: 61  FGFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPL--P 120
           FGF+TF+S+ESVDSVLQ NFHELNGRRVEVKRAIPKKGNYN  HIPNAR    KSPL   
Sbjct: 111 FGFVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNAR--AAKSPLLNG 170

Query: 121 NRNVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAP 180
           NRNVPFPYPYYGSGY IYPGFT FPHYG   VD YGSNFYGNWFPP   N+ASDG W+ P
Sbjct: 171 NRNVPFPYPYYGSGYPIYPGFTHFPHYGA-AVDSYGSNFYGNWFPPGSANIASDGWWHGP 230

Query: 181 NTFGVGMFPPPYRNSLIPPSYSSKGISDTGTGNGMA-IRNGEIKQNTSGKRSMSADALPF 240
           N F VGM PP  RNSL+ P   SKGI   G+ NGMA  RNGEIKQNT+GKR MSADA PF
Sbjct: 231 NFFSVGMLPPLCRNSLMHP---SKGIY-IGSRNGMANTRNGEIKQNTAGKRIMSADAAPF 289

Query: 241 QIKDRT 244
           QIKDRT
Sbjct: 291 QIKDRT 289

BLAST of Lsi04G014570 vs. NCBI nr
Match: XP_004141412.1 (RNA-binding protein 1 [Cucumis sativus])

HSP 1 Score: 363.2 bits (931), Expect = 1.7e-96
Identity = 190/244 (77.87%), Postives = 203/244 (83.20%), Query Frame = 0

Query: 3   MSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFG 62
           MS  RM+NNNDQCRGRKIFVGGLSAELTE+EF++YFE FG+ITDVVVMHDSVTNRPRGFG
Sbjct: 1   MSTTRMDNNNDQCRGRKIFVGGLSAELTEEEFRSYFENFGQITDVVVMHDSVTNRPRGFG 60

Query: 63  FITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPL--PNR 122
           F+TF+S+ESVDSVLQ NFHELNGRRVEVKRAIPKKGNYN  HIPNAR    KSPL   NR
Sbjct: 61  FVTFESLESVDSVLQRNFHELNGRRVEVKRAIPKKGNYNGVHIPNAR--AAKSPLLNGNR 120

Query: 123 NVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNT 182
           NVPFPYPYYGSGY IYPGFT FPHYG   VD YGSNFYGNWFPP   N+ASDG W+ PN 
Sbjct: 121 NVPFPYPYYGSGYPIYPGFTHFPHYGA-AVDSYGSNFYGNWFPPGSANIASDGWWHGPNF 180

Query: 183 FGVGMFPPPYRNSLIPPSYSSKGISDTGTGNGMA-IRNGEIKQNTSGKRSMSADALPFQI 242
           F VGM PP  RNSL+ P   SKGI   G+ NGMA  RNGEIKQNT+GKR MSADA PFQI
Sbjct: 181 FSVGMLPPLCRNSLMHP---SKGIY-IGSRNGMANTRNGEIKQNTAGKRIMSADAAPFQI 237

Query: 243 KDRT 244
           KDRT
Sbjct: 241 KDRT 237

BLAST of Lsi04G014570 vs. NCBI nr
Match: XP_022982410.1 (RNA-binding protein 1-like [Cucurbita maxima])

HSP 1 Score: 313.2 bits (801), Expect = 2.0e-81
Identity = 166/241 (68.88%), Postives = 184/241 (76.35%), Query Frame = 0

Query: 3   MSN-IRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGF 62
           MSN  RME   DQ RGRKIFVGGLSAELTED+FKNYFEKFG++TDVVVM DSVT RPRGF
Sbjct: 1   MSNGTRME---DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGF 60

Query: 63  GFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRN 122
           GFITFDSV+SVDSVLQNNFHELNGRRVEVKRAIPKKGNYN GHI        KSPL   N
Sbjct: 61  GFITFDSVQSVDSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-------SKSPLSESN 120

Query: 123 VPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNTF 182
           V   YPYY  GYT+YPGFTPFP Y GPG  LYGS+ YGNWF PSFG MAS  AWY PN F
Sbjct: 121 VLPSYPYYSCGYTVYPGFTPFPPY-GPGFGLYGSDPYGNWFSPSFGPMASPAAWYGPNFF 180

Query: 183 GVGMFPPPYRNSLIPPSYSSKGISDTGTGNGMAIRNGEIKQNTSGKRSMSADALPFQIKD 242
             GMFPPP++NSL+ P+YSS G+S    G   A    +++  T  +++MSADA+PF  KD
Sbjct: 181 --GMFPPPHKNSLVYPTYSSNGLSTPVAGYETADARRKVEPPTRAQKNMSADAVPFHAKD 228

BLAST of Lsi04G014570 vs. NCBI nr
Match: XP_022940975.1 (RNA-binding protein 1-like [Cucurbita moschata])

HSP 1 Score: 312.0 bits (798), Expect = 4.5e-81
Identity = 160/230 (69.57%), Postives = 178/230 (77.39%), Query Frame = 0

Query: 13  DQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDSVESV 72
           DQ RGRKIFVGGLSAELTED+FKNYFEKFG++TDVVVM DSVT RPRGFGFITFDSV+SV
Sbjct: 9   DQFRGRKIFVGGLSAELTEDDFKNYFEKFGRVTDVVVMRDSVTKRPRGFGFITFDSVQSV 68

Query: 73  DSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPYYGSG 132
           DSVLQNNFHELNGRRVEVKRAIPKKGNYN GHI        KSPL   NV   YPYY  G
Sbjct: 69  DSVLQNNFHELNGRRVEVKRAIPKKGNYNGGHI-------SKSPLSESNVLPSYPYYSCG 128

Query: 133 YTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGNMASDGAWYAPNTFGVGMFPPPYRN 192
           YT+YPGFTPFP Y GPG  LYGS+ YGNWF PSFG MAS  AWYAPN F  GMFPPP++N
Sbjct: 129 YTVYPGFTPFPPY-GPGFGLYGSDPYGNWFSPSFGPMASPAAWYAPNFF--GMFPPPHKN 188

Query: 193 SLIPPSYSSKGISDTGTGNGMAIRNGEIKQNTSGKRSMSADALPFQIKDR 243
           SL+ P+YSS G+S    G   A    ++   T  +++MSADA+PF  KD+
Sbjct: 189 SLVYPTYSSNGLSTPVAGYETADARRKVDPPTRAQKNMSADAVPFHAKDK 228

BLAST of Lsi04G014570 vs. TAIR 10
Match: AT1G58470.1 (RNA-binding protein 1 )

HSP 1 Score: 139.8 bits (351), Expect = 2.9e-33
Identity = 75/145 (51.72%), Postives = 101/145 (69.66%), Query Frame = 0

Query: 1   MNMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRG 60
           MN     M +N    R +KIFVGGLS+  TE+EFK+YFE+FG+ TDVVVMHD VTNRPRG
Sbjct: 103 MNGGLREMSSNGVTSRTKKIFVGGLSSNTTEEEFKSYFERFGRTTDVVVMHDGVTNRPRG 162

Query: 61  FGFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKKG----NYNVGHIPNARTQGGKSP 120
           FGF+T+DS +SV+ V+Q+NFHEL+ +RVEVKRAIPK+G    N N  +IP + +    +P
Sbjct: 163 FGFVTYDSEDSVEVVMQSNFHELSDKRVEVKRAIPKEGIQSNNGNAVNIPPSYSSFQATP 222

Query: 121 -LPNRN-----VPFPYPYYGSGYTI 136
            +P +N     + FP P +G  + +
Sbjct: 223 YVPEQNGYGMVLQFPPPVFGYHHNV 247

BLAST of Lsi04G014570 vs. TAIR 10
Match: AT4G26650.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-29
Identity = 75/158 (47.47%), Postives = 91/158 (57.59%), Query Frame = 0

Query: 11  NNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDSVE 70
           N    R +KIFVGGL + +TE EFKNYF++FG I DVVVM+D  T RPRGFGFITFDS E
Sbjct: 115 NGGGARTKKIFVGGLPSSITEAEFKNYFDQFGTIADVVVMYDHNTQRPRGFGFITFDSEE 174

Query: 71  SVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPYYG 130
           SVD VL   FHELNG+ VEVKRA+PK+ +               S  PNR+   P   YG
Sbjct: 175 SVDMVLHKTFHELNGKMVEVKRAVPKELS---------------STTPNRS---PLIGYG 234

Query: 131 SGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGN 169
           + Y + P  +              +N Y N FPP + N
Sbjct: 235 NNYGVVPNRS-------------SANSYFNSFPPGYNN 241

BLAST of Lsi04G014570 vs. TAIR 10
Match: AT4G26650.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-29
Identity = 75/158 (47.47%), Postives = 91/158 (57.59%), Query Frame = 0

Query: 11  NNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGFGFITFDSVE 70
           N    R +KIFVGGL + +TE EFKNYF++FG I DVVVM+D  T RPRGFGFITFDS E
Sbjct: 112 NGGGARTKKIFVGGLPSSITEAEFKNYFDQFGTIADVVVMYDHNTQRPRGFGFITFDSEE 171

Query: 71  SVDSVLQNNFHELNGRRVEVKRAIPKKGNYNVGHIPNARTQGGKSPLPNRNVPFPYPYYG 130
           SVD VL   FHELNG+ VEVKRA+PK+ +               S  PNR+   P   YG
Sbjct: 172 SVDMVLHKTFHELNGKMVEVKRAVPKELS---------------STTPNRS---PLIGYG 231

Query: 131 SGYTIYPGFTPFPHYGGPGVDLYGSNFYGNWFPPSFGN 169
           + Y + P  +              +N Y N FPP + N
Sbjct: 232 NNYGVVPNRS-------------SANSYFNSFPPGYNN 238

BLAST of Lsi04G014570 vs. TAIR 10
Match: AT5G47620.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.2e-28
Identity = 86/207 (41.55%), Postives = 108/207 (52.17%), Query Frame = 0

Query: 2   NMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGF 61
           N SN  ++ +      +KIFVGGL++ +TE EFK YF +FG ITDVVVM+D  T RPRGF
Sbjct: 90  NKSNSSLQGSPGPSNSKKIFVGGLASSVTEAEFKKYFAQFGMITDVVVMYDHRTQRPRGF 149

Query: 62  GFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKK---------------GNYNVGHIP 121
           GFI++DS E+VD VLQ  FHELNG+ VEVK A+PK                G   +  + 
Sbjct: 150 GFISYDSEEAVDKVLQKTFHELNGKMVEVKLAVPKDMALNTMRNQMNVNSFGTSRISSLL 209

Query: 122 NARTQG-GKSPLPNRNVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNF-------Y 181
           N  TQG   SP+    V  P   Y        GF+PF H  G G++L   NF       Y
Sbjct: 210 NEYTQGFSPSPISGYGVK-PEVRYSPAVGNRGGFSPFGH--GYGIEL---NFEPNQTQNY 269

Query: 182 GNWFPPSFGNMASDGAWYAPNTFGVGM 186
           G+     FG   S G   +   FG  M
Sbjct: 270 GSGSSGGFGRPFSPGYAASLGRFGSQM 290

BLAST of Lsi04G014570 vs. TAIR 10
Match: AT5G47620.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.2e-28
Identity = 86/207 (41.55%), Postives = 108/207 (52.17%), Query Frame = 0

Query: 2   NMSNIRMENNNDQCRGRKIFVGGLSAELTEDEFKNYFEKFGKITDVVVMHDSVTNRPRGF 61
           N SN  ++ +      +KIFVGGL++ +TE EFK YF +FG ITDVVVM+D  T RPRGF
Sbjct: 90  NKSNSSLQGSPGPSNSKKIFVGGLASSVTEAEFKKYFAQFGMITDVVVMYDHRTQRPRGF 149

Query: 62  GFITFDSVESVDSVLQNNFHELNGRRVEVKRAIPKK---------------GNYNVGHIP 121
           GFI++DS E+VD VLQ  FHELNG+ VEVK A+PK                G   +  + 
Sbjct: 150 GFISYDSEEAVDKVLQKTFHELNGKMVEVKLAVPKDMALNTMRNQMNVNSFGTSRISSLL 209

Query: 122 NARTQG-GKSPLPNRNVPFPYPYYGSGYTIYPGFTPFPHYGGPGVDLYGSNF-------Y 181
           N  TQG   SP+    V  P   Y        GF+PF H  G G++L   NF       Y
Sbjct: 210 NEYTQGFSPSPISGYGVK-PEVRYSPAVGNRGGFSPFGH--GYGIEL---NFEPNQTQNY 269

Query: 182 GNWFPPSFGNMASDGAWYAPNTFGVGM 186
           G+     FG   S G   +   FG  M
Sbjct: 270 GSGSSGGFGRPFSPGYAASLGRFGSQM 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6524.0e-3251.72RNA-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBP1 PE=2 SV=1[more]
Q8W0347.4e-2634.75Heterogeneous nuclear ribonucleoprotein 1 OS=Arabidopsis thaliana OX=3702 GN=RNP... [more]
Q96EP51.3e-1935.68DAZ-associated protein 1 OS=Homo sapiens OX=9606 GN=DAZAP1 PE=1 SV=1[more]
Q98SJ21.8e-1932.20DAZ-associated protein 1 OS=Xenopus laevis OX=8355 GN=dazap1 PE=1 SV=1[more]
Q920Q61.8e-1941.55RNA-binding protein Musashi homolog 2 OS=Mus musculus OX=10090 GN=Msi2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L5638.3e-9777.87RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G644730 PE=4 SV... [more]
A0A6J1J2K59.9e-8268.88RNA-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481242 PE=4 SV=1[more]
A0A6J1FS872.2e-8169.57RNA-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446397 PE=4 SV... [more]
A0A6J1C7242.0e-6658.96RNA-binding protein 1-like OS=Momordica charantia OX=3673 GN=LOC111008498 PE=4 S... [more]
A0A5N6R9D75.5e-4849.53Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_013735 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038898856.19.7e-12489.07RNA-binding protein 1-like [Benincasa hispida][more]
KAE8649895.12.6e-9777.64hypothetical protein Csa_012205 [Cucumis sativus][more]
XP_004141412.11.7e-9677.87RNA-binding protein 1 [Cucumis sativus][more]
XP_022982410.12.0e-8168.88RNA-binding protein 1-like [Cucurbita maxima][more]
XP_022940975.14.5e-8169.57RNA-binding protein 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G58470.12.9e-3351.72RNA-binding protein 1 [more]
AT4G26650.11.5e-2947.47RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G26650.21.5e-2947.47RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G47620.11.2e-2841.55RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G47620.21.2e-2841.55RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 19..91
e-value: 5.1E-26
score: 102.4
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 20..88
e-value: 1.8E-17
score: 63.0
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 18..95
score: 18.95611
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 15..103
e-value: 1.5E-27
score: 97.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 206..235
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 206..231
NoneNo IPR availablePANTHERPTHR48032RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6coord: 7..232
NoneNo IPR availablePANTHERPTHR48032:SF12NUCLEOTIDE-BINDING ALPHA-BETA PLAIT DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 7..232
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 16..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G014570.1Lsi04G014570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding