Lsi04G013550 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G013550
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Protein FAM33A
Location	chr04: 21292386 .. 21297143 (-)
RNA-Seq Expression	Lsi04G013550
Synteny	Lsi04G013550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAGAAAAAACAACGTATTTTAATTTTTTTGGCCATTGGATGTAGGAGAGAGGAGCCACAATTTTTTTTATTGAGTAGTTATTAAAAATATGCAAATGATTGAATTCACATCTAACGATTTGTCGGCTCACAATTATTAAGAGACAGTTACATTATGGTGATCTTATTAACCAAAGCCTTCGAATCATTGGATCTGGACTGAGTTTGGGCTATCAATTTTTGAATCCGCCCAATGATGAGAGCGACCAACACTAGTTAATGACATGAATTTCAACAATTACAAGTACATTAATTAGGTTTTCAATTCAAAATGTTTTTGCAATTTTGCAATAGTGTCATTTATTTGATTTTACAACTTAAGAAGGCTATTTTCCTTGAAAAAACTATTTCGAGGCATATAACACGTGGCGCAAAGTAAATGCATCCATCACATCACCCCAATCTTTCTTTTGAAAGAAGGCAAATACATAGAATCCATTGGCGCGGAAGGAGGTTCAAATTAAGAGAGCGCGAAAATCCTAGCAGAGAGAGAAGATGGGGTTCGATCATCTTCATCAGCATCATCAAGCGGCCGATGGATTGACGAACTTGTTCACCAAGGCCAATCACGATCTCTCGGTGGTTCAGTACAGGCTCGAGAAGGAGTTTCAGCAAATATATCCTGAGAGCGTAAGATCAACACGATCTTTTTCTTTTTCTTCTTCTTCTACTCGTTGATTGAATGAAGGCACTAATTAGATTAAGGAGGAAGTTTTAGGGCTTGAAATGAATGTAGAATTGTTTACGTCTCTCTTTCGTGTTAGTATTTCACAGTTCTAGTACCAGATCTTTGAGCTTAATGTATAATTACTTCATTCTACAGCGTGAGAGCATCATAGGCTTTCTCTATCTTCTTAGCATCATAGGCTTTCTCTATCTTCTTAATTGAGGCACCGTTTGGTTTGAGGATGGAGTTGAAGATGTGGAGCTCTATGTTTGAACTATTTTGTTCTTTCTTTGCTGTTATTTCACTGTACTAACACAAGATCATGAGCTTAATGTAGAATTATTCAATTCGACAGCGTAGGAACAAGTAATCTATCATGACAGAGTAAGAGCAACATATGTTTTTCTTCTTCTTCTAATTTTTCTTCTGAGTGGAGGCACTATTTGGATTGAGGAGGACATTCGAGTTATTGAGCTTAATGAAGATTTATTTCATTATCTTTTGGCTATTGGCATTTCACAAAGATCTTCAGCTGCTCATGAGCTAAACGTAGAATTATTTCGTTCTACAGTGTAAGAGCAAGGAATCTATCACGACAGGGTTGGAGCAACAATTTTTTTTTTAATTTTTTGTCGAGGCACTATTTGGTTGGAGGAGGAAGTAGTAGCTAGATTTTGCGCTTAATGAAGAGTGATTTCATTCTTCTATTAGAACCGCAGTACTATTGCAAGATTTTGAGGTTATCGTAGAATTGTGAATTGTTCCCTTCTCTCTTGCCAGTCAGTTTATTTCATACTACTAACCAACAGTATGTTGTGGTTATTGATTTTAAAAGGTTCGTGTCACAGAATAAATATTGTTTGTTCTGAGTTTGGATTTCGTAATTTTCAGGTCGAAGTTTTTGGAAAATTTGTTCACCCACACTTTCTTCATTTTCGAAATTCTGATTCTAGTAAGCTTTTTTATGCTTGGTAACACGTCGGATTATTGCTGTTTGTTTCTCTATAATGTATGCAACTATGTGGCAGTGAAAATGGATTCAAGCACACTTTATGATAAAGATTGTTCGGTCTTTGTTTGACGGTTGAGACTAGGAATTCTCTTAGAACATGTAATGGGATGGGGCTTAAATATGTCGTACAAAATATACGTCGTTGCTGCACAAAGTTAGCCACAGATTAATGTGGAATTCGGTCTCATATTTTCCTCCATATTCTTTCTAGAAAATACAAACACGAATCTGTTGTTGCTCAAGTGGTGGTTTTCATTCAAGATTTTGTAAATTGTTTACAGGCGAATCCAATGAAGTTAGTTTCAAGGATAAAGAAGATACGAGATGATATATCAACCTTGAAAGAGCAGTGCCGTGAAGTTTTGATGGCCAAACAGGTACTTTTCTTCATTTACTTTCTCCAGAAAAGTATTGACTAAAGGAAACATACGCGTTTAAATTGTTAGATCATGCTCTATATATAAAGTTTTTTTCCCCTCAAGTATTAGGTCGGCAACTCTATTTCACTTAGATTCAATGTGATGAAATGTTGATATATTATATCTAGAAAAATGATAATATGAAGTTTGGGAATTTGATGTCTGATGAAAATAGGATGAATTGTATTTGGTGGCCGGTGGGTGGAAATGTGATTTAGATTTTTATTATATATAAGTTAAAATCTAATGGTATGCTTAATAATATTTACATCGAGAATAATTTGTTTAACGTGCACACTGAATTCAAAATTATTTCTAACAAATAATTATAAATGAGACTACGGTTTCTTTTAATAACCACATTGTTTATAGTTTCTGGTTTTGAAATTTATGATTGTTCCTTCTCAATCTTTTTACCATAGTTTCACTTTCTTGGAGAAATATTTGATTTTTTTTTTTTTTTGAAACGGAAACTAGGAGAAATATTTGAATTCTGAGTCAAATTTCAAAAACAAAAACAAGTTTTCAAAATTGTATCTTTTAGTTTTCAAAATTTGGCTTGGTTATTGATAACATGGATAGATTGTGGATAACAAAACAAACAAACTTATAGGTGAAATAGTAGTGTTTATAAGTTTAATTTCTAAAAATTAAAACTAGGACTTCGTTTGATAACCAGTTGGTTTTAAATTTTTTGCTTTTGAAAATTAAGTTTATAAACATTACTCTTTCACATATAAGTTTCTTTGTTTTATTATTTACTTTCTAATATTGTTTTCAAAAAGCAACCCAAGTTTTGAAAAAATAGAACAGAATTTTAGTTAATGTATTGATAGTTGGTGCCTCTTTTACTCTTTTATCGGAGGACGAGGATTCAAAAAGACATTGGTAATTGGAGGAACGGCTTTGACAATTACACTTTTTTTTCTTGTTCTGGAACTGAAGTCGGTTGGACTTGATACCTTTTACTAATTTTTTTTTAGAAGGAATGACCCTGACTTGTACTGTATTATTCTTGATCTGGAACTGGGATTGAGTGATTGCAATACCTATTCTGCTAATTTATGCAACGATGATGTCCTGTTCAAATGCAGGATGTAATTGATAAGGCACGGACGGTTCTGGTTGGGAACAGAAACATGCTGCAAAAAATGCAAGCATCCATGGGCGTCCCCTTTGTGAGCGATCTCGATGATTCTGCATTTGTGCAATTCAAGCAGGTATTCATTGTTAATGTCCCTTGGTTTGCATTTTGTGGTCAGTACTCGTGCAACGCATTGATTATTCATTTGGCAATAGCCTGAATTCCTTTACTATGCTTGTGCAAAAGATTGCAGCTTTTAGATTTCATGTCACCCTGTACTCATAGTCTTATCTTAAGTTTTTGGTGTACCTCTTCTTTGTGCCATTTCATTTTTCACTTAACATCTGTACAAAGCTAATACAAGGTTCTTATTTATTCAAAATTTTATTCTTCTTTAGCTATTAGAAATGTTAACGCAGTGAAGTGGACATTTTTGTCTCAAGTGCTCTATTATCTGCTTTATCGTGATTTAAAAGGTTGCAATTTCCAATTGGTGCGTATAGTATTTGTACCATTTGCCAGCAAGCAACCAATATTAAATATGCAAAAGGCTTTTCTTAGGATTTGATATCCACATTAGATGGTATTGTGGGTATTTCGATCTTTTGGATAAAGCTTACACAACCAAATGTCTCACCCTCTCCATGTAGAAGCGTCGTGTTTTTAAGTATTTGCAGAGTCATTGTTTAATGCTAAACCATTTCCTGAATTCCCTCCTGCAGATCACTGAAGAGTGGACAACACAAGTTCAATCAAAATTTGGTAAGGATTCTTTTCAAGCTTTCTACAGACATTCCGATGCAGATAAATTGTTTGTGCTTATTAGCAAATGGGCATGCATAGTTCGTTACTTGCTGCTTAATTAGTATTTACGATCTAAATTTAGAACCAAATCGAATTCCAACTTTCAACCTTCCCAGTAAAAAATCAAGTCACTACCACTCGAATCATAGATTATAGTTTTTTTCCCCTCAATATGGAGTGAAAGAATCAAACTTCTGATCTTGTAGGATGAAGGAATCAAATTTCTGATCTCGAAGTCGATAGTAAGCTTTATGTAGATTGAGCTATGCTCATGTTGGCATTGATTACCGTTATCCCCTATCCGTTCTGGCACTTAGTTAATGTTTAGACCTATCCCATCTTTTAAACTCTAGCCAATTTACGAAAATGTTAAAAAATATATATATATGTGATGACAACCCTAAAAAGTTCAATAATGGTTGCATTTGCTGCAATGTCGAAACTAAAGTTTGTTTCTCCTTTCTTACTTAACTTTTCGTTGTGTTTTTCTTAAAAATATGGGGATTATGAGTTTTTTTCTTTTCACCGATTATGAATGAAGATCAAACTATTGACTTTTAAGATGGTAATTAGAATCATCTGAAATTTTATTTTGGATATGTTCAAATGTGTTAATATTATATGTGTGTGCAAATTGTGTGTTGGCGCTAATGCAGGGGAAGATGGCCAAGAGGAAGCCGACTGTAATGACGTCAACAAGTTGCTATTCTCAGCCGTTGTGCAGGCATGA

mRNA sequence

AAAAAAGAAAAAACAACGTATTTTAATTTTTTTGGCCATTGGATGTAGGAGAGAGGAGCCACAATTTTTTTTATTGAGTAGTTATTAAAAATATGCAAATGATTGAATTCACATCTAACGATTTGTCGGCTCACAATTATTAAGAGACAGTTACATTATGGTGATCTTATTAACCAAAGCCTTCGAATCATTGGATCTGGACTGAGTTTGGGCTATCAATTTTTGAATCCGCCCAATGATGAGAGCGACCAACACTAGTTAATGACATGAATTTCAACAATTACAAGTACATTAATTAGGTTTTCAATTCAAAATGTTTTTGCAATTTTGCAATAGTGTCATTTATTTGATTTTACAACTTAAGAAGGCTATTTTCCTTGAAAAAACTATTTCGAGGCATATAACACGTGGCGCAAAGTAAATGCATCCATCACATCACCCCAATCTTTCTTTTGAAAGAAGGCAAATACATAGAATCCATTGGCGCGGAAGGAGGTTCAAATTAAGAGAGCGCGAAAATCCTAGCAGAGAGAGAAGATGGGGTTCGATCATCTTCATCAGCATCATCAAGCGGCCGATGGATTGACGAACTTGTTCACCAAGGCCAATCACGATCTCTCGGTGGTTCAGTACAGGCTCGAGAAGGAGTTTCAGCAAATATATCCTGAGAGCGCGAATCCAATGAAGTTAGTTTCAAGGATAAAGAAGATACGAGATGATATATCAACCTTGAAAGAGCAGTGCCGTGAAGTTTTGATGGCCAAACAGGATGTAATTGATAAGGCACGGACGGTTCTGGTTGGGAACAGAAACATGCTGCAAAAAATGCAAGCATCCATGGGCGTCCCCTTTGTGAGCGATCTCGATGATTCTGCATTTGTGCAATTCAAGCAGGTATTCATTGTTAATGTCCCTTGGTTTGCATTTTGTGGGGAAGATGGCCAAGAGGAAGCCGACTGTAATGACGTCAACAAGTTGCTATTCTCAGCCGTTGTGCAGGCATGA

Coding sequence (CDS)

ATGGGGTTCGATCATCTTCATCAGCATCATCAAGCGGCCGATGGATTGACGAACTTGTTCACCAAGGCCAATCACGATCTCTCGGTGGTTCAGTACAGGCTCGAGAAGGAGTTTCAGCAAATATATCCTGAGAGCGCGAATCCAATGAAGTTAGTTTCAAGGATAAAGAAGATACGAGATGATATATCAACCTTGAAAGAGCAGTGCCGTGAAGTTTTGATGGCCAAACAGGATGTAATTGATAAGGCACGGACGGTTCTGGTTGGGAACAGAAACATGCTGCAAAAAATGCAAGCATCCATGGGCGTCCCCTTTGTGAGCGATCTCGATGATTCTGCATTTGTGCAATTCAAGCAGGTATTCATTGTTAATGTCCCTTGGTTTGCATTTTGTGGGGAAGATGGCCAAGAGGAAGCCGACTGTAATGACGTCAACAAGTTGCTATTCTCAGCCGTTGTGCAGGCATGA

Protein sequence

MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRDDISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQVFIVNVPWFAFCGEDGQEEADCNDVNKLLFSAVVQA

Homology

[Close]

BLAST of Lsi04G013550 vs. ExPASy Swiss-Prot
Match: Q8WVK7 (Spindle and kinetochore-associated protein 2 OS=Homo sapiens OX=9606 GN=SKA2 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.3e-04
Identity = 28/88 (31.82%), Postives = 47/88 (53.41%), Query Frame = 0


Query: 14 DGLTNLFTKANHDLSVVQYRLEKEFQQIYPESA---NPMKLVSRIKKIRDDISTLKEQCR 73
          D L  +F KA  DL  +QYRLE E +  +P+SA   NP+ L+  +  I+    TL  + +
Sbjct: 6  DKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTLYARFK 65

Query: 74 EVLMAKQDVIDKARTVLVGNRNMLQKMQ 99
           V + +++   +    +    NM+QK+Q
Sbjct: 66 PVAVEQKESKSRICATVKKTMNMIQKLQ 93

[Close]

BLAST of Lsi04G013550 vs. ExPASy Swiss-Prot
Match: Q4R8E8 (Spindle and kinetochore-associated protein 2 OS=Macaca fascicularis OX=9541 GN=SKA2 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.9e-04
Identity = 26/88 (29.55%), Postives = 47/88 (53.41%), Query Frame = 0


Query: 14 DGLTNLFTKANHDLSVVQYRLEKEFQQIYPESA---NPMKLVSRIKKIRDDISTLKEQCR 73
          D L  +F KA  DL  +QYRLE E +  +P+SA   NP+ L+  +  ++    TL  + +
Sbjct: 6  DKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSALKSRYQTLYARFK 65

Query: 74 EVLMAKQDVIDKARTVLVGNRNMLQKMQ 99
           V + +++   +    +    N++QK+Q
Sbjct: 66 PVAVEQKETKSRICATVNKTMNVIQKLQ 93

[Close]

BLAST of Lsi04G013550 vs. ExPASy Swiss-Prot
Match: Q2TBY0 (Spindle and kinetochore-associated protein 2 OS=Bos taurus OX=9913 GN=SKA2 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 6.6e-04
Identity = 29/90 (32.22%), Postives = 50/90 (55.56%), Query Frame = 0


Query: 14 DGLTNLFTKANHDLSVVQYRLEKEFQQIYPESA---NPMKLVSRIKKIRDDISTLKEQCR 73
          D L  +F KA+ DL  +QYRLE E +  YP+SA   NP+ L+  +  I+    TL  + +
Sbjct: 6  DKLELMFQKADSDLDYIQYRLEYEIKTNYPDSAGKKNPVTLLKELSAIKSRYQTLHVRFK 65

Query: 74 EVLMAKQDVIDKARTVLVGNRNM--LQKMQ 99
           + + +++   K+R     N+ M  +Q++Q
Sbjct: 66 PIAVEQKET--KSRICATFNKTMTLIQELQ 93

[Close]

BLAST of Lsi04G013550 vs. ExPASy TrEMBL
Match: A0A0A0L5I9 (Protein FAM33A OS=Cucumis sativus OX=3659 GN=Csa_4G651780 PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.8e-63
Identity = 132/161 (81.99%), Postives = 140/161 (86.96%), Query Frame = 0


Query: 1   MGFD-HLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIR 60
           MG D HL QHHQAADGL NLFTKAN DLSVVQYRLEKEFQQIYPESANPMKLVSRIKKI+
Sbjct: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60

Query: 61  DDISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQ 120
           DDISTLKEQCREVL+AKQDVID+ARTVL+GNRNMLQKMQASMG+PFVSDLDD AF +FKQ
Sbjct: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120

Query: 121 VFIVNVPWFA-----FCGEDGQEEADCNDVNKLLFSAVVQA 156
              +   W       F GEDGQE+ADCNDVNKLLFSAVVQA
Sbjct: 121 ---ITEEWTTQVQSKFSGEDGQEDADCNDVNKLLFSAVVQA 158

[Close]

BLAST of Lsi04G013550 vs. ExPASy TrEMBL
Match: A0A6J1CJF1 (Protein FAM33A OS=Momordica charantia OX=3673 GN=LOC111011710 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 5.0e-63
Identity = 128/157 (81.53%), Postives = 137/157 (87.26%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           MG  HLHQHHQAADGL NLFTKANHDLSVVQYRLEKEFQQIYP+SANPMKLVSRIKKIRD
Sbjct: 1   MGLHHLHQHHQAADGLANLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLVSRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTL+EQCRE+LMAKQDVIDKARTVLVGNRNMLQKMQASMG+P VSDLDD AF QFK+ 
Sbjct: 61  DISTLQEQCREILMAKQDVIDKARTVLVGNRNMLQKMQASMGIPLVSDLDDHAFAQFKK- 120

Query: 121 FIVNVPWF----AFCGEDGQEEADCNDVNKLLFSAVV 154
             +   W     +  G+DGQE+AD NDVNKLLFSAVV
Sbjct: 121 --ITEEWTTQVQSKIGDDGQEDADSNDVNKLLFSAVV 154

[Close]

BLAST of Lsi04G013550 vs. ExPASy TrEMBL
Match: A0A6J1F6V9 (Protein FAM33A OS=Cucurbita moschata OX=3662 GN=LOC111442663 PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 6.5e-63
Identity = 130/158 (82.28%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           MG DHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYP+SANPMKL  RIKKIRD
Sbjct: 1   MGLDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLALRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTLKEQCREVLMAKQDVIDKARTVLVGNRNM+ KMQASMG PFVSD DD AFVQFKQ 
Sbjct: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMVHKMQASMGKPFVSDDDDPAFVQFKQ- 120

Query: 121 FIVNVPWF----AFCGEDGQEEADCNDVNKLLFSAVVQ 155
             +   W     +  GEDGQE+A+ +DVNKLLFSAVVQ
Sbjct: 121 --ITEEWTTQVQSKIGEDGQEDAESSDVNKLLFSAVVQ 155

[Close]

BLAST of Lsi04G013550 vs. ExPASy TrEMBL
Match: A0A6J1FBS9 (Protein FAM33A OS=Cucurbita moschata OX=3662 GN=LOC111442663 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.6e-61
Identity = 128/156 (82.05%), Postives = 134/156 (85.90%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           MG DHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYP+SANPMKL  RIKKIRD
Sbjct: 1   MGLDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLALRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTLKEQCREVLMAKQDVIDKARTVLVGNRNM+ KMQASMG PFVSD DD AFVQFKQ 
Sbjct: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMVHKMQASMGKPFVSDDDDPAFVQFKQ- 120

Query: 121 FIVNVPWFAFCGE--DGQEEADCNDVNKLLFSAVVQ 155
             +   W        DGQE+A+ +DVNKLLFSAVVQ
Sbjct: 121 --ITEEWTTQVQSKIDGQEDAESSDVNKLLFSAVVQ 153

[Close]

BLAST of Lsi04G013550 vs. ExPASy TrEMBL
Match: A0A6J1IP41 (Protein FAM33A OS=Cucurbita maxima OX=3661 GN=LOC111477290 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.7e-61
Identity = 128/158 (81.01%), Postives = 136/158 (86.08%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           M  D LHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYP+SANPMKL  RIKKIRD
Sbjct: 1   MALDRLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLALRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTLKEQCR+VLMAKQDVIDKARTVLVGNRNML+KMQASMG PFVSD DD AFVQFKQ 
Sbjct: 61  DISTLKEQCRKVLMAKQDVIDKARTVLVGNRNMLRKMQASMGKPFVSDDDDPAFVQFKQ- 120

Query: 121 FIVNVPWF----AFCGEDGQEEADCNDVNKLLFSAVVQ 155
             +   W     +  GEDGQE+A+ +DVNKLLFSAVVQ
Sbjct: 121 --ITEEWTTQVQSKIGEDGQEDAESSDVNKLLFSAVVQ 155

[Close]

BLAST of Lsi04G013550 vs. NCBI nr
Match: XP_038898183.1 (spindle and kinetochore-associated protein 2 [Benincasa hispida] >XP_038898184.1 spindle and kinetochore-associated protein 2 [Benincasa hispida])

HSP 1 Score: 260.4 bits (664), Expect = 1.0e-65
Identity = 136/159 (85.53%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           M  DHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD
Sbjct: 1   MVLDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQK+QASMGVPFVSDLDD AF +FKQ 
Sbjct: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKLQASMGVPFVSDLDDPAFAEFKQ- 120

Query: 121 FIVNVPWF----AFCGEDGQEEADCNDVNKLLFSAVVQA 156
             +   W     +  GEDGQE+AD NDVNKLLFSAVVQA
Sbjct: 121 --ITEEWTRQVQSKFGEDGQEDADYNDVNKLLFSAVVQA 156

[Close]

BLAST of Lsi04G013550 vs. NCBI nr
Match: KAG6591515.1 (hypothetical protein SDJN03_13861, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 253.8 bits (647), Expect = 9.4e-64
Identity = 131/158 (82.91%), Postives = 138/158 (87.34%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           MG DHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYP+SANPMKL  RIKKIRD
Sbjct: 1   MGLDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLALRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTLKEQCREVLMAKQDVIDKARTVLVGNRNM+ KMQASMG PFVSD DD AFVQFKQ 
Sbjct: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMVHKMQASMGKPFVSDDDDPAFVQFKQ- 120

Query: 121 FIVNVPWF----AFCGEDGQEEADCNDVNKLLFSAVVQ 155
             +   W     +  GEDGQE+A+C+DVNKLLFSAVVQ
Sbjct: 121 --ITEEWTTQVQSKIGEDGQEDAECSDVNKLLFSAVVQ 155

[Close]

BLAST of Lsi04G013550 vs. NCBI nr
Match: XP_004145489.1 (spindle and kinetochore-associated protein 2 [Cucumis sativus] >KGN55411.1 hypothetical protein Csa_012758 [Cucumis sativus])

HSP 1 Score: 250.8 bits (639), Expect = 7.9e-63
Identity = 132/161 (81.99%), Postives = 140/161 (86.96%), Query Frame = 0


Query: 1   MGFD-HLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIR 60
           MG D HL QHHQAADGL NLFTKAN DLSVVQYRLEKEFQQIYPESANPMKLVSRIKKI+
Sbjct: 1   MGIDHHLDQHHQAADGLMNLFTKANLDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIQ 60

Query: 61  DDISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQ 120
           DDISTLKEQCREVL+AKQDVID+ARTVL+GNRNMLQKMQASMG+PFVSDLDD AF +FKQ
Sbjct: 61  DDISTLKEQCREVLIAKQDVIDQARTVLLGNRNMLQKMQASMGIPFVSDLDDPAFSEFKQ 120

Query: 121 VFIVNVPWFA-----FCGEDGQEEADCNDVNKLLFSAVVQA 156
              +   W       F GEDGQE+ADCNDVNKLLFSAVVQA
Sbjct: 121 ---ITEEWTTQVQSKFSGEDGQEDADCNDVNKLLFSAVVQA 158

[Close]

BLAST of Lsi04G013550 vs. NCBI nr
Match: XP_022141272.1 (spindle and kinetochore-associated protein 2 [Momordica charantia])

HSP 1 Score: 250.4 bits (638), Expect = 1.0e-62
Identity = 128/157 (81.53%), Postives = 137/157 (87.26%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           MG  HLHQHHQAADGL NLFTKANHDLSVVQYRLEKEFQQIYP+SANPMKLVSRIKKIRD
Sbjct: 1   MGLHHLHQHHQAADGLANLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLVSRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTL+EQCRE+LMAKQDVIDKARTVLVGNRNMLQKMQASMG+P VSDLDD AF QFK+ 
Sbjct: 61  DISTLQEQCREILMAKQDVIDKARTVLVGNRNMLQKMQASMGIPLVSDLDDHAFAQFKK- 120

Query: 121 FIVNVPWF----AFCGEDGQEEADCNDVNKLLFSAVV 154
             +   W     +  G+DGQE+AD NDVNKLLFSAVV
Sbjct: 121 --ITEEWTTQVQSKIGDDGQEDADSNDVNKLLFSAVV 154

[Close]

BLAST of Lsi04G013550 vs. NCBI nr
Match: XP_022935897.1 (spindle and kinetochore-associated protein 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 250.0 bits (637), Expect = 1.4e-62
Identity = 130/158 (82.28%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MGFDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRD 60
           MG DHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYP+SANPMKL  RIKKIRD
Sbjct: 1   MGLDHLHQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPDSANPMKLALRIKKIRD 60

Query: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQV 120
           DISTLKEQCREVLMAKQDVIDKARTVLVGNRNM+ KMQASMG PFVSD DD AFVQFKQ 
Sbjct: 61  DISTLKEQCREVLMAKQDVIDKARTVLVGNRNMVHKMQASMGKPFVSDDDDPAFVQFKQ- 120

Query: 121 FIVNVPWF----AFCGEDGQEEADCNDVNKLLFSAVVQ 155
             +   W     +  GEDGQE+A+ +DVNKLLFSAVVQ
Sbjct: 121 --ITEEWTTQVQSKIGEDGQEDAESSDVNKLLFSAVVQ 155

[Close]

BLAST of Lsi04G013550 vs. TAIR 10
Match: AT2G24970.1 (unknown protein; Has 33 Blast hits to 33 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 28; Viruses - 0; Other Eukaryotes - 4 (source: NCBI BLink). )

HSP 1 Score: 147.9 bits (372), Expect = 6.7e-36
Identity = 74/146 (50.68%), Postives = 102/146 (69.86%), Query Frame = 0


Query: 7   HQHHQAADGLTNLFTKANHDLSVVQYRLEKEFQQIYPESANPMKLVSRIKKIRDDISTLK 66
           + HHQA D L N+F++A+HDL+VV  +L+KEFQQ+YP +ANPMKL+ RIKK+++D++ LK
Sbjct: 4   NHHHQAVDNLLNVFSRASHDLTVVHSKLDKEFQQMYPANANPMKLIQRIKKLQEDVTLLK 63

Query: 67  EQCREVLMAKQDVIDKARTVLVGNRNMLQKMQASMGVPFVSDLDDSAFVQFKQVFIVNVP 126
            QC ++L AKQD+IDKA+T LVGN N++QKM AS+G     D DD A   F Q+      
Sbjct: 64  HQCLDLLSAKQDLIDKAQTTLVGNCNLIQKMNASLGESTNGDTDD-ALADFNQIIDEWTM 123

Query: 127 WFAFCGEDGQEEADCNDVNKLLFSAV 153
                     E+AD  D+NK+LFSA+
Sbjct: 124 QVRSRTVGETEDADKEDINKMLFSAI 148

The following BLAST results are available for this feature:

BLAST of Lsi04G013550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8WVK7	1.3e-04	31.82	Spindle and kinetochore-associated protein 2 OS=Homo sapiens OX=9606 GN=SKA2 PE=...	[more]
Q4R8E8	3.9e-04	29.55	Spindle and kinetochore-associated protein 2 OS=Macaca fascicularis OX=9541 GN=S...	[more]
Q2TBY0	6.6e-04	32.22	Spindle and kinetochore-associated protein 2 OS=Bos taurus OX=9913 GN=SKA2 PE=2 ...	[more]

BLAST of Lsi04G013550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5I9	3.8e-63	81.99	Protein FAM33A OS=Cucumis sativus OX=3659 GN=Csa_4G651780 PE=3 SV=1	[more]
A0A6J1CJF1	5.0e-63	81.53	Protein FAM33A OS=Momordica charantia OX=3673 GN=LOC111011710 PE=3 SV=1	[more]
A0A6J1F6V9	6.5e-63	82.28	Protein FAM33A OS=Cucurbita moschata OX=3662 GN=LOC111442663 PE=3 SV=1	[more]
A0A6J1FBS9	1.6e-61	82.05	Protein FAM33A OS=Cucurbita moschata OX=3662 GN=LOC111442663 PE=3 SV=1	[more]
A0A6J1IP41	2.7e-61	81.01	Protein FAM33A OS=Cucurbita maxima OX=3661 GN=LOC111477290 PE=3 SV=1	[more]

BLAST of Lsi04G013550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898183.1	1.0e-65	85.53	spindle and kinetochore-associated protein 2 [Benincasa hispida] >XP_038898184.1...	[more]
KAG6591515.1	9.4e-64	82.91	hypothetical protein SDJN03_13861, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_004145489.1	7.9e-63	81.99	spindle and kinetochore-associated protein 2 [Cucumis sativus] >KGN55411.1 hypot...	[more]
XP_022141272.1	1.0e-62	81.53	spindle and kinetochore-associated protein 2 [Momordica charantia]	[more]
XP_022935897.1	1.4e-62	82.28	spindle and kinetochore-associated protein 2 isoform X1 [Cucurbita moschata]	[more]

BLAST of Lsi04G013550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G24970.1	6.7e-36	50.68	unknown protein; Has 33 Blast hits to 33 proteins in 17 species: Archae - 0; Bac...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042091	Ska2, N-terminal	PFAM	PF16740	SKA2	coord: 10..117 e-value: 3.5E-41 score: 139.5
None	No IPR available	GENE3D	6.10.250.1380		coord: 10..120 e-value: 9.4E-27 score: 95.3
IPR026762	Spindle and kinetochore-associated protein 2	PANTHER	PTHR32017	SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 2	coord: 12..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G013550.1	Lsi04G013550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0007059	chromosome segregation
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000940	outer kinetochore
cellular_component	GO:0005876	spindle microtubule
molecular_function	GO:0008017	microtubule binding