Lsi04G012810 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G012810
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDUF4408 domain-containing protein
Locationchr04: 20232247 .. 20233358 (-)
RNA-Seq ExpressionLsi04G012810
SyntenyLsi04G012810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTCTGTAATGACTTCAAGCAGCCATTGGATTCTATCCCTCAAGCTCCTACTCCTCTCCTCCGCCCTTCTATCCATGGCGGTGATTCTGAAGTTCTACGTTCCGGTGGTTGCCGGTATCCTTCTCTCCGACGTTCCCGCCGTTTGGAGCTCCATCGTTTTCTGGTTAAAACCTCCTTACCTTTACTTGCTCGTTAACTTCATTATCATCAGTATCCTCGCCTCCTCCAAGCTTCATAAGGACCTGGTGGATCCTCCGCCTACGACAATGCCAAAGCCGTCCGCTGATTCACAAATGGATTTCACAGTAAGTAGAGCCGTCGTCAGCGATGTTCTCATGAAGCGCGGTCGCGGTGCGAACGAAAACTTCGCGGCGGATGTTTCGGAAGATGAAGGGAAATCGGAGTACTCTAGGGTTTCAGACAGAGAGATTGATGAAAACAGCGAGACAGATCGGAGGCGCCATTGCAAAAAGAGGATTCGATGGAGAATTTGTTGAAGACGACGTACGATAAGAAACCGCCGATTTCTTCAAAGGTTGGTAATCGGAAAGCTGCAAAAGCTGCTGCTACTCCTGCAGGTGCTACTCTCTAGTCCTTTCTCTCTCTCTCTCTTTCTCTGCAACGCCATGATTATTTTTCAGAAACTGTAGAATCTGATGGCAGAATCGTAATTAATTTGATTTTCGGAGGGTGAATTCCTGGTGCAGGGACGACAACGAGATGGTCCAGAATGAACGGACACGACACGTTGGACGACGCGTGGAAGGCAATAACGGAGGGCGAGTCGGCGCCGTTCTCGAGACAGTTAGATAAATCCGATACGTGGGAGTTACATACGAAACGGGACGAAGAAGAAGAAACCGCCATAGGCCGAAATAGTAGTAGCCCTCCGAAAGTGACGAAATCGGAAACTACTTTCAACGGCCAGGAGTTGAAGTTGAGGCAATCGGCGACGGAGGGGAGAGTGAGAAAAATGCCGTCGCTGACGGAGGAGGAATTGAACCGGCGGGTGGAGGCGTTTATAGAGAAGTTTAAGGAAGAAATGAGGCTGCAGAGGGAGGAATCACTGAAGAAATTTGAGGTGATGGTAAAAGGAATCGATTATTGA

mRNA sequence

ATGCTCTCTGTAATGACTTCAAGCAGCCATTGGATTCTATCCCTCAAGCTCCTACTCCTCTCCTCCGCCCTTCTATCCATGGCGGTGATTCTGAAGTTCTACGTTCCGGTGGTTGCCGGTATCCTTCTCTCCGACGTTCCCGCCGTTTGGAGCTCCATCGTTTTCTGGTTAAAACCTCCTTACCTTTACTTGCTCGTTAACTTCATTATCATCAGTATCCTCGCCTCCTCCAAGCTTCATAAGGACCTGGTGGATCCTCCGCCTACGACAATGCCAAAGCCGTCCGCTGATTCACAAATGGATTTCACAGTAAGTAGAGCCGTCGTCAGCGATGTTCTCATGAAGCGCGGTCGCGGTGCGAACGAAAACTTCGCGGCGGATGTTTCGGAAGATGAAGGGAAATCGGAGTACTCTAGGACGACGTACGATAAGAAACCGCCGATTTCTTCAAAGGTTGGTAATCGGAAAGCTGCAAAAGCTGCTGCTACTCCTGCAGGGACGACAACGAGATGGTCCAGAATGAACGGACACGACACGTTGGACGACGCGTGGAAGGCAATAACGGAGGGCGAGTCGGCGCCGTTCTCGAGACAGTTAGATAAATCCGATACGTGGGAGTTACATACGAAACGGGACGAAGAAGAAGAAACCGCCATAGGCCGAAATAGTAGTAGCCCTCCGAAAGTGACGAAATCGGAAACTACTTTCAACGGCCAGGAGTTGAAGTTGAGGCAATCGGCGACGGAGGGGAGAGTGAGAAAAATGCCGTCGCTGACGGAGGAGGAATTGAACCGGCGGGTGGAGGCGTTTATAGAGAAGTTTAAGGAAGAAATGAGGCTGCAGAGGGAGGAATCACTGAAGAAATTTGAGGTGATGGTAAAAGGAATCGATTATTGA

Coding sequence (CDS)

ATGCTCTCTGTAATGACTTCAAGCAGCCATTGGATTCTATCCCTCAAGCTCCTACTCCTCTCCTCCGCCCTTCTATCCATGGCGGTGATTCTGAAGTTCTACGTTCCGGTGGTTGCCGGTATCCTTCTCTCCGACGTTCCCGCCGTTTGGAGCTCCATCGTTTTCTGGTTAAAACCTCCTTACCTTTACTTGCTCGTTAACTTCATTATCATCAGTATCCTCGCCTCCTCCAAGCTTCATAAGGACCTGGTGGATCCTCCGCCTACGACAATGCCAAAGCCGTCCGCTGATTCACAAATGGATTTCACAGTAAGTAGAGCCGTCGTCAGCGATGTTCTCATGAAGCGCGGTCGCGGTGCGAACGAAAACTTCGCGGCGGATGTTTCGGAAGATGAAGGGAAATCGGAGTACTCTAGGACGACGTACGATAAGAAACCGCCGATTTCTTCAAAGGTTGGTAATCGGAAAGCTGCAAAAGCTGCTGCTACTCCTGCAGGGACGACAACGAGATGGTCCAGAATGAACGGACACGACACGTTGGACGACGCGTGGAAGGCAATAACGGAGGGCGAGTCGGCGCCGTTCTCGAGACAGTTAGATAAATCCGATACGTGGGAGTTACATACGAAACGGGACGAAGAAGAAGAAACCGCCATAGGCCGAAATAGTAGTAGCCCTCCGAAAGTGACGAAATCGGAAACTACTTTCAACGGCCAGGAGTTGAAGTTGAGGCAATCGGCGACGGAGGGGAGAGTGAGAAAAATGCCGTCGCTGACGGAGGAGGAATTGAACCGGCGGGTGGAGGCGTTTATAGAGAAGTTTAAGGAAGAAATGAGGCTGCAGAGGGAGGAATCACTGAAGAAATTTGAGGTGATGGTAAAAGGAATCGATTATTGA

Protein sequence

MLSVMTSSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFIIISILASSKLHKDLVDPPPTTMPKPSADSQMDFTVSRAVVSDVLMKRGRGANENFAADVSEDEGKSEYSRTTYDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWKAITEGESAPFSRQLDKSDTWELHTKRDEEEETAIGRNSSSPPKVTKSETTFNGQELKLRQSATEGRVRKMPSLTEEELNRRVEAFIEKFKEEMRLQREESLKKFEVMVKGIDY
Homology
BLAST of Lsi04G012810 vs. ExPASy TrEMBL
Match: A0A6J1IHJ9 (uncharacterized protein LOC111475963 OS=Cucurbita maxima OX=3661 GN=LOC111475963 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 8.9e-93
Identity = 227/416 (54.57%), Postives = 259/416 (62.26%), Query Frame = 0

Query: 1   MLSVMTSSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPP 60
           MLSVMTS+S WIL L+LL LSSA+LSM  ILKFYVP+V  IL+SDVPAVWSSIV WL+PP
Sbjct: 1   MLSVMTSNSLWILFLRLLFLSSAILSMEEILKFYVPLVVDILVSDVPAVWSSIVLWLRPP 60

Query: 61  YLYLLVNFIIISILASSKLHKDLVDPPPTTMPKPSADSQMDFTVSRAVVSDVLMKRGRGA 120
           YLYLL NFII+SILASSKLH+ LVDPPPTT+PK   DS+ DF V RA V DVL K GR A
Sbjct: 61  YLYLLFNFIIVSILASSKLHQYLVDPPPTTVPKLYDDSRADFRVIRA-VGDVLEKNGRDA 120

Query: 121 NENFAADVSEDEGKSE-------------------------------------------- 180
           +E+FAADVSEDEGKS+                                            
Sbjct: 121 DEDFAADVSEDEGKSDAELYSKISDGENDENEETDRSRLQNEDSVENLLQKYDKKPLKLY 180

Query: 181 -------------------------------------------YSRTT------------ 240
                                                      YS+ +            
Sbjct: 181 ADSRADFTVIRAVGDVLEKSDRDADEDFAADVSEDEEKSDAELYSKISDRENDGNGETGR 240

Query: 241 ----------------YDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWK 299
                           YDKKPPIS K+GNRKAAKAA TPAGTT++WS MNGHDTLDDAW 
Sbjct: 241 SRLQKEDYLVEKLLQKYDKKPPISLKIGNRKAAKAATTPAGTTSKWSTMNGHDTLDDAWN 300

BLAST of Lsi04G012810 vs. ExPASy TrEMBL
Match: A0A6J1F789 (uncharacterized protein LOC111442999 OS=Cucurbita moschata OX=3662 GN=LOC111442999 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.5e-92
Identity = 207/306 (67.65%), Postives = 234/306 (76.47%), Query Frame = 0

Query: 27  MAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFIIISILASSKLHKDLVDP 86
           MA ILKFYVP+V  IL+S VPAVWSSIV WL+PPYLYLL NFII+SILASSKLH+ LVDP
Sbjct: 1   MAEILKFYVPLVVDILVSYVPAVWSSIVLWLRPPYLYLLFNFIIVSILASSKLHQYLVDP 60

Query: 87  PPTTMPKPSADSQMDFTVSRAVVSDVLMKRGRGANENFAADVSEDEGKSE---YSRTT-- 146
           PPTT+PK   DS+ DFTV RA V DVL K GR A+E+FAADVSEDE KS+   YS+ +  
Sbjct: 61  PPTTVPKLYDDSRADFTVIRA-VGDVLEKSGRDADEDFAADVSEDEEKSDAELYSKISDR 120

Query: 147 -------------------------YDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNG 206
                                    YDKKPPISSK+ NRKAAKAA TPAGTT+RWS +N 
Sbjct: 121 ENDGNGETDRSRLQKEDSVENLLQKYDKKPPISSKIRNRKAAKAATTPAGTTSRWSTINR 180

Query: 207 HDTLDDAWKAITEGESAPFSRQLDKSDTWELHTKR--DEEEETAIGRNSSSPPKVTKSET 266
           HDTLDDAW AITEG SAPFSR+LDKS+T E HT R  +EEEETAIG+N +SPPKVTKSET
Sbjct: 181 HDTLDDAWNAITEGHSAPFSRRLDKSNTCEPHTTRNEEEEEETAIGQN-NSPPKVTKSET 240

Query: 267 TF--NGQELKLRQSATEGRVRKMPSLTEEELNRRVEAFIEKFKEEMRLQREESLKKFEVM 299
           +F  N +  +LRQSA   RVRKMPS+TEEELNRRVEAFIEKF+EEMRLQREESLKKFE+M
Sbjct: 241 SFKSNDKNQELRQSAAAARVRKMPSVTEEELNRRVEAFIEKFREEMRLQREESLKKFELM 300

BLAST of Lsi04G012810 vs. ExPASy TrEMBL
Match: A0A2P5B6Z8 (DUF4408 domain-containing protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_265650 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 9.0e-45
Identity = 132/343 (38.48%), Postives = 192/343 (55.98%), Query Frame = 0

Query: 7   SSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLV 66
           SS+ W LSLK++L+S+ +LSMAV LK  VPVV   + S+VP++WS ++ WLKPPYLY+++
Sbjct: 8   SSNSWALSLKVVLISTGVLSMAVALKLSVPVVTDFVASEVPSLWSFVLSWLKPPYLYVVI 67

Query: 67  NFIIISILASSKLHKDLVDPPP------TTMPKPSAD---------SQMDFTVSRAVVSD 126
           N IIISI+ASSKLH+ + +PPP        +P  +A+         ++ D+ +   V   
Sbjct: 68  NCIIISIVASSKLHQKVDEPPPPLISPAMMVPPVTAEAVKISGGEVARTDYAMYSGV--- 127

Query: 127 VLMKRGRGANENFAA--------------------------DVSEDEGKS---------- 186
           VL   G   N+  AA                          +V +DE  +          
Sbjct: 128 VLTGYGYDPNDVAAAAAKVSEAHAPPPRVAEKEELFVMNGGEVEDDEAVAASLAWNGLQR 187

Query: 187 ----EYSRTTYDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWKAITEGE 246
               E   +  ++KPP+S++ G+RK+ K A+   G + R S+    DTL+  WK ITEG 
Sbjct: 188 KDSLEICFSNENEKPPVSARFGHRKSVK-ASPEGGKSLRVSKPKRQDTLESTWKTITEGR 247

Query: 247 SAPFSRQLDKSDTWELHTKRDEEEETAIGRNSSSPPKVTKSETTFNGQELKLRQSATEGR 295
           S P +R L KSDTWE H +R+     A    SS PP   K   + +  EL     +  G+
Sbjct: 248 SMPLTRHLKKSDTWESHVRRNARTAAAAEMFSSPPPAAEKFHNSSSATELSPASRSGAGK 307

BLAST of Lsi04G012810 vs. ExPASy TrEMBL
Match: W9RGE1 (DUF4408 domain-containing protein OS=Morus notabilis OX=981085 GN=L484_018748 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 5.9e-44
Identity = 134/338 (39.64%), Postives = 184/338 (54.44%), Query Frame = 0

Query: 8   SSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVN 67
           S++WIL LK++L+S+ +LSMAV LK  VPVV   + S+VP+ WS ++ WLKPPYLY+L+N
Sbjct: 9   SNNWILPLKVVLISTGVLSMAVALKLSVPVVVDFVASEVPSFWSFVLSWLKPPYLYVLIN 68

Query: 68  FIIISILASSKLHKDLVDPPPTTMPKPSADSQMDFTVS-------RAVVSDVLMKRGRGA 127
            IIISI+ASSKLH+ L D  P+T P   A +  +  +S        AV S V++  G G 
Sbjct: 69  GIIISIVASSKLHQKLED--PSTSPSHVAITAGEVKISGGEVRTDYAVYSGVVL-TGYGY 128

Query: 128 NENFAADVSEDEGKS------------------------------------------EYS 187
           +    A VS    +S                                          EY 
Sbjct: 129 DPGEVAKVSAMTAESVVYAPAARVVEASVSAAEKEEREEAIVAASERNGLQRKDSSLEYF 188

Query: 188 RTTYDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWKAITEGESAPFSRQ 247
             + ++KPP+S++ G+RKA KA+    G   R S+    DTL+  WK ITEG   P +R 
Sbjct: 189 FPSENEKPPVSARFGHRKAVKASPEGGGKALRVSKPKRQDTLESTWKTITEGRPMPLTRH 248

Query: 248 LDKSDTWELHTKRDEEEETAIGRNSSSPPKVTKSETTF--NGQELKLRQSATEGRVRKMP 295
           L KSDTWE H +R      +    S+ PP   K   TF        L  S   G++RK P
Sbjct: 249 LKKSDTWESHVRRGGVGIQSQNDQSTPPPAKMKKSETFADRNPNSSLSPSPGSGKLRKEP 308

BLAST of Lsi04G012810 vs. ExPASy TrEMBL
Match: A0A498KDZ0 (DUF4408 domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_004335 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.0e-43
Identity = 135/350 (38.57%), Postives = 195/350 (55.71%), Query Frame = 0

Query: 6   TSSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLL 65
           +SS++W+L+LK+ L+S+ ++SMAV LK   P+VA  + S VP++WSS + WL+PPYLY+L
Sbjct: 4   SSSNNWLLTLKVALISTGVVSMAVGLKLSSPLVADAVTSQVPSLWSSAISWLRPPYLYIL 63

Query: 66  VNFIIISILASSKLH-----------KDLVDPPPTTMPKPSADSQMDFTVSRAV------ 125
           +N IIISI+ASSKLH            +LV  PP T  K S   Q D+     V      
Sbjct: 64  INCIIISIVASSKLHPKPEDSHPETEPELVMVPPLTTEKISTQIQSDYAAYDGVILSEYG 123

Query: 126 ---------------------------VSDVLMKRGR--------GANENFAADVS---- 185
                                      VS+ + K+          G +EN          
Sbjct: 124 YDANVLPKISDSYGGGTGGVVLEEKVWVSEAVKKKENREDRAAIDGGDENALVSTPVKTS 183

Query: 186 -EDEGKSEYSRTTYDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWKAIT 245
            + +G  E+     ++KPP+S+++G+RK+ K A+   G +   S+   HDTL+  W+ IT
Sbjct: 184 LQRKGSMEF-WLNENQKPPVSARIGHRKSVK-ASPEGGKSLGVSKPKRHDTLESTWRTIT 243

Query: 246 EGESAPFSRQLDKSDTWELHTKRDEEEETAIGRNSSSPP--KVTKSET---TFNGQELKL 294
           +G S P +R L KSDTWE H +R++E        ++ PP  KV KSET     +G    L
Sbjct: 244 DGRSMPLTRHLKKSDTWETHVRRNDE--------NTPPPKSKVNKSETFQERSSGNSPSL 303

BLAST of Lsi04G012810 vs. NCBI nr
Match: XP_023535113.1 (uncharacterized protein LOC111796630 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 384.4 bits (986), Expect = 8.8e-103
Identity = 231/331 (69.79%), Postives = 258/331 (77.95%), Query Frame = 0

Query: 1   MLSVMTSSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPP 60
           MLSVMTS+S WIL LKLLLLSS +LSMA ILKFYVP+V  IL+SDVPAVWSSIV WL+PP
Sbjct: 1   MLSVMTSNSLWILFLKLLLLSSGILSMAEILKFYVPLVVDILVSDVPAVWSSIVLWLRPP 60

Query: 61  YLYLLVNFIIISILASSKLHKDLVDPPPTTMPKPSADSQMDFTVSRAVVSDVLMKRGRGA 120
           YLYLL NFII+SILASSKLH+ LVDPPPTTM K  ADS+ DFTV RA V DVL K GR A
Sbjct: 61  YLYLLFNFIIVSILASSKLHQYLVDPPPTTMSKLYADSRADFTVIRA-VDDVLEKSGRDA 120

Query: 121 NENFAADVSEDEGKSE---YSRTT---------------------------YDKKPPISS 180
           +E+FAADVSEDE KS+   YS+ +                           YDKKPPISS
Sbjct: 121 DEDFAADVSEDEEKSDAELYSKISDRENDENGETDRSRLQKEDSVEKLLQKYDKKPPISS 180

Query: 181 KVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWKAITEGESAPFSRQLDKSDTWELHTK 240
           K+ NRKAAKAA TPAGTT+RWS MN HDTLDDAW AITEG SAPFSR+LDKS+T E HT 
Sbjct: 181 KIRNRKAAKAATTPAGTTSRWSTMNRHDTLDDAWNAITEGHSAPFSRRLDKSNTCESHTT 240

Query: 241 R-DEEEETAIGRNSSSPPKVTKSETTF--NGQELKLRQSATEGRVRKMPSLTEEELNRRV 299
           R +EEEETAIG+N +SPPKVTKSET+F  N +  +LR+SA   RVRKMPS+TEEELNRRV
Sbjct: 241 RNEEEEETAIGQN-NSPPKVTKSETSFKNNDKNQELRRSAAAARVRKMPSVTEEELNRRV 300

BLAST of Lsi04G012810 vs. NCBI nr
Match: KAG6591991.1 (hypothetical protein SDJN03_14337, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024866.1 hypothetical protein SDJN02_13685 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 378.6 bits (971), Expect = 4.8e-101
Identity = 228/332 (68.67%), Postives = 258/332 (77.71%), Query Frame = 0

Query: 1   MLSVMTSSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPP 60
           ML +MTS+S WILSLKLLLLSSA+LSMA ILKFYVP+V  IL+S VPAVWSSIV WL+PP
Sbjct: 1   MLYLMTSNSLWILSLKLLLLSSAILSMAEILKFYVPLVVDILVSYVPAVWSSIVLWLRPP 60

Query: 61  YLYLLVNFIIISILASSKLHKDLVDPPPTTMPKPSADSQMDFTVSRAVVSDVLMKRGRGA 120
           YLYLL NFII+SILASSKLH+ LVDPPPTT+PK   DS+ DFTV RA V DVL K GR A
Sbjct: 61  YLYLLFNFIIVSILASSKLHQYLVDPPPTTVPKLYDDSRADFTVIRA-VGDVLEKSGRDA 120

Query: 121 NENFAADVSEDEGKSE---YSRTT---------------------------YDKKPPISS 180
           +E+FAADVSEDE KS+   YS+ +                           YDKKPPISS
Sbjct: 121 DEDFAADVSEDEEKSDAELYSKISDRENDGNGETDRSRLQKEDSVENLLQKYDKKPPISS 180

Query: 181 KVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWKAITEGESAPFSRQLDKSDTWELHTK 240
           K+ NRKAAKAA TPAGTT+RWS +N HDTLDDAW AITEG SAPFSR+LDKS+T E HT 
Sbjct: 181 KIRNRKAAKAATTPAGTTSRWSTINRHDTLDDAWNAITEGHSAPFSRRLDKSNTCEPHTT 240

Query: 241 R--DEEEETAIGRNSSSPPKVTKSETTF--NGQELKLRQSATEGRVRKMPSLTEEELNRR 299
           R  +EEEETAIG+N +SPPKVTKSET+F  N +  +LRQSA   RVRKMPS+TEEELNRR
Sbjct: 241 RNEEEEEETAIGQN-NSPPKVTKSETSFKSNDKNQELRQSAAAARVRKMPSVTEEELNRR 300

BLAST of Lsi04G012810 vs. NCBI nr
Match: XP_022975735.1 (uncharacterized protein LOC111475963 [Cucurbita maxima])

HSP 1 Score: 350.1 bits (897), Expect = 1.8e-92
Identity = 227/416 (54.57%), Postives = 259/416 (62.26%), Query Frame = 0

Query: 1   MLSVMTSSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPP 60
           MLSVMTS+S WIL L+LL LSSA+LSM  ILKFYVP+V  IL+SDVPAVWSSIV WL+PP
Sbjct: 1   MLSVMTSNSLWILFLRLLFLSSAILSMEEILKFYVPLVVDILVSDVPAVWSSIVLWLRPP 60

Query: 61  YLYLLVNFIIISILASSKLHKDLVDPPPTTMPKPSADSQMDFTVSRAVVSDVLMKRGRGA 120
           YLYLL NFII+SILASSKLH+ LVDPPPTT+PK   DS+ DF V RA V DVL K GR A
Sbjct: 61  YLYLLFNFIIVSILASSKLHQYLVDPPPTTVPKLYDDSRADFRVIRA-VGDVLEKNGRDA 120

Query: 121 NENFAADVSEDEGKSE-------------------------------------------- 180
           +E+FAADVSEDEGKS+                                            
Sbjct: 121 DEDFAADVSEDEGKSDAELYSKISDGENDENEETDRSRLQNEDSVENLLQKYDKKPLKLY 180

Query: 181 -------------------------------------------YSRTT------------ 240
                                                      YS+ +            
Sbjct: 181 ADSRADFTVIRAVGDVLEKSDRDADEDFAADVSEDEEKSDAELYSKISDRENDGNGETGR 240

Query: 241 ----------------YDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWK 299
                           YDKKPPIS K+GNRKAAKAA TPAGTT++WS MNGHDTLDDAW 
Sbjct: 241 SRLQKEDYLVEKLLQKYDKKPPISLKIGNRKAAKAATTPAGTTSKWSTMNGHDTLDDAWN 300

BLAST of Lsi04G012810 vs. NCBI nr
Match: XP_022936351.1 (uncharacterized protein LOC111442999 [Cucurbita moschata])

HSP 1 Score: 349.4 bits (895), Expect = 3.1e-92
Identity = 207/306 (67.65%), Postives = 234/306 (76.47%), Query Frame = 0

Query: 27  MAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFIIISILASSKLHKDLVDP 86
           MA ILKFYVP+V  IL+S VPAVWSSIV WL+PPYLYLL NFII+SILASSKLH+ LVDP
Sbjct: 1   MAEILKFYVPLVVDILVSYVPAVWSSIVLWLRPPYLYLLFNFIIVSILASSKLHQYLVDP 60

Query: 87  PPTTMPKPSADSQMDFTVSRAVVSDVLMKRGRGANENFAADVSEDEGKSE---YSRTT-- 146
           PPTT+PK   DS+ DFTV RA V DVL K GR A+E+FAADVSEDE KS+   YS+ +  
Sbjct: 61  PPTTVPKLYDDSRADFTVIRA-VGDVLEKSGRDADEDFAADVSEDEEKSDAELYSKISDR 120

Query: 147 -------------------------YDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNG 206
                                    YDKKPPISSK+ NRKAAKAA TPAGTT+RWS +N 
Sbjct: 121 ENDGNGETDRSRLQKEDSVENLLQKYDKKPPISSKIRNRKAAKAATTPAGTTSRWSTINR 180

Query: 207 HDTLDDAWKAITEGESAPFSRQLDKSDTWELHTKR--DEEEETAIGRNSSSPPKVTKSET 266
           HDTLDDAW AITEG SAPFSR+LDKS+T E HT R  +EEEETAIG+N +SPPKVTKSET
Sbjct: 181 HDTLDDAWNAITEGHSAPFSRRLDKSNTCEPHTTRNEEEEEETAIGQN-NSPPKVTKSET 240

Query: 267 TF--NGQELKLRQSATEGRVRKMPSLTEEELNRRVEAFIEKFKEEMRLQREESLKKFEVM 299
           +F  N +  +LRQSA   RVRKMPS+TEEELNRRVEAFIEKF+EEMRLQREESLKKFE+M
Sbjct: 241 SFKSNDKNQELRQSAAAARVRKMPSVTEEELNRRVEAFIEKFREEMRLQREESLKKFELM 300

BLAST of Lsi04G012810 vs. NCBI nr
Match: KAF3453608.1 (hypothetical protein FNV43_RR04048 [Rhamnella rubrinervis])

HSP 1 Score: 190.7 bits (483), Expect = 1.9e-44
Identity = 139/355 (39.15%), Postives = 194/355 (54.65%), Query Frame = 0

Query: 6   TSSSHWILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLL 65
           TSS++WILSLKL+L+S+ ++SMAV+LK  VPVVA  +  +VP++W  ++ WL+PPYLY+L
Sbjct: 9   TSSNNWILSLKLILISTGVVSMAVVLKLSVPVVADFVAYEVPSIWGVLLSWLRPPYLYIL 68

Query: 66  VNFIIISILASSKLHK----------DLVDPPPTT-----MPKPSADSQMDFTVSRAVVS 125
           +N IIISI+ASSKL +          ++V P  TT     +     D + D+ V   VV 
Sbjct: 69  INGIIISIVASSKLQQKAEYSTTPAAEMVVPTMTTTEALKVSSSGGDVRKDYAVYNGVVF 128

Query: 126 DVLMKRGRGANE----------------------------------------NFAADVSE 185
           +     G GANE                                           ADVS 
Sbjct: 129 N---GYGYGANEVAKVADSHVEDLQRWAGVADDKVPEVEKKMNELPPMAMKAGDVADVSA 188

Query: 186 DEGKS-------EYSRTTYDKKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDA 245
             G         E+S +  ++KPP+S++ G+RKA K A+   G +   S+    DTL+  
Sbjct: 189 SFGNGLQRKDSMEFSFSNENEKPPVSARFGHRKAVK-ASPEGGKSLGVSKPKRQDTLEST 248

Query: 246 WKAITEGESAPFSRQLDKSDTWELHTKRDEEEETAIGRNSSSPPKVTKSETTFN----GQ 295
           WK IT+G S P +R L KSDTWE H +R+ + +       S+PPK  K   TFN    G 
Sbjct: 249 WKMITDGRSMPLTRHLKKSDTWETHGRRNTQVD------QSTPPKAMKKSETFNDRSGGA 308

BLAST of Lsi04G012810 vs. TAIR 10
Match: AT1G61260.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 114.0 bits (284), Expect = 2.1e-25
Identity = 110/352 (31.25%), Postives = 167/352 (47.44%), Query Frame = 0

Query: 11  WILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFII 70
           W+++ K +L+SS + ++A++LK  VPV     +S  P +WSS++ WLKPPYLY++ N II
Sbjct: 4   WMMTTKAVLISSGVATVALLLKLSVPVAVDFSVSRAPILWSSLLSWLKPPYLYVVTNGII 63

Query: 71  ISILASSKLHKDLVD----------------------------PPPTTMPKPSADSQMDF 130
           I+I+ASSK ++   D                              P  +     D+   F
Sbjct: 64  ITIVASSKYYRSHHDRDEEDEIVVYGGGGYKIQTEEPIVNQHQASPRILEVKDLDTGAHF 123

Query: 131 ---------------TVSRAVVSDVLMKRGRGANENFAADVSEDEGKSEYSRTTYD---- 190
                           V+  V  D   ++    +   A D  E+E KS       D    
Sbjct: 124 GFVVANLEAEELESEAVTAVVFDDEEEEKKIIDSAATAEDEIEEELKSVIMVENSDLVES 183

Query: 191 -----------------KKPPISSKVGNRKAAKAAATPAGTTTRWSRMNGHDTLDDAWKA 250
                            +KP ++S+ G+RK  K A+   G   R ++   ++TL++ WK 
Sbjct: 184 DVIPPPMMIESENLPPIEKPLVTSRFGHRKLMK-ASQEGGRALRVTKPKKNETLENTWKM 243

Query: 251 ITEGESAPFSRQL-DKSDTWELHTKRDEEEETAIGRNSSSPPKVTKSETTFNGQELKLRQ 297
           ITEG+S P +RQL  +SDT+            + G +    P   KS+T          Q
Sbjct: 244 ITEGKSTPLTRQLYRRSDTF--------GRGDSGGVDGEVKPVYKKSDTF--RDRTNYYQ 303

BLAST of Lsi04G012810 vs. TAIR 10
Match: AT5G54300.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-23
Identity = 109/323 (33.75%), Postives = 150/323 (46.44%), Query Frame = 0

Query: 26  SMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFIIISILASSKL-HK--- 85
           S+A  +   VP V+  ++S  P ++ + VF LKPPYLYL++N II+ I+A+SKL HK   
Sbjct: 23  SIATAMILTVPSVSHFVVSCFPIIYDNTVFLLKPPYLYLVINSIIVCIIATSKLTHKSSS 82

Query: 86  -------DLVDPPPTTMP---KPSADS--------QMDFTVSRAVVSDVLMKRGRGANEN 145
                  ++V P P  +P       DS          D+T     + DV +     A   
Sbjct: 83  VDDSEISEVVTPIPIPVPVHLPSDIDSGYLNVVHVVSDYTGFVEKIDDVSINPTVEAIRK 142

Query: 146 F----AADVSEDEGKSEYSRTTYDK----------------KPP---------ISSKVGN 205
           F     A+ S++   S    T   K                KPP          +S+ GN
Sbjct: 143 FPEVQEAEKSKESSDSPEPETEKPKLKNDSPEISILKHSTRKPPRFNQQKSLKSNSEGGN 202

Query: 206 RKAAKAAATPAGTTTRWSRMNGHDTLDDAWKAITEGESAPFSRQLDKSDTWELHTKRDEE 265
           +K A     P             DTL+  WK ITEG S P ++ L KSDTW        +
Sbjct: 203 KKTALGVTKPP---------RRQDTLETTWKKITEGRSTPLTKHLTKSDTW--------Q 262

Query: 266 EETAIGRNSSSPPKVTKSETTFNGQELKLRQSATEGR--VRKMPSLTEEELNRRVEAFIE 296
           E   +  +  +  K+TKSE       LK   + TE +  +++ PS  +EELNRRVEAFI+
Sbjct: 263 ERAHVQSSPENKEKMTKSE------NLKDINTPTEEKTVLKREPSPGQEELNRRVEAFIK 322

BLAST of Lsi04G012810 vs. TAIR 10
Match: AT1G11220.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 75.9 bits (185), Expect = 6.2e-14
Identity = 89/321 (27.73%), Postives = 145/321 (45.17%), Query Frame = 0

Query: 12  ILSLKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFIII 71
           ++S+K  L+++ ++++++ LK  VP+     +S  P  WSS + WLKPPYL++ +N II 
Sbjct: 5   MISIKAALITAGIVAVSLFLKSSVPIAVDFSVSRFPIFWSSFLSWLKPPYLFVAINVIIT 64

Query: 72  SILASSKLHKD----------------------LVDPPPTTMPKPSADSQMDFTVSRAVV 131
            I+ASSK ++                       +   PP  +    AD     TV   ++
Sbjct: 65  IIMASSKFYQSVGEQDGEDDEILLGGEYTIPNVITQAPPRRLVDLDADFDFVATVQSPIL 124

Query: 132 -------------SDVLMKRGRGANENFAADVSE--DEGKSEYSRTTYDKKPPISSKVGN 191
                         ++ +       + FA   SE       E       +KP +S++ G+
Sbjct: 125 VAEVEILEVVFEEKEMAISGQTNGGDEFAVMRSELNQPIMEESENLPPAEKPLVSARSGH 184

Query: 192 RKAAKAAATPAGTTTRWSRM---NGHDTLDDAWKAIT-EGESAPFSRQLDKSDTWELHTK 251
           RK  KA++       +  ++   N H+TL++ W  IT EG+S P +    K+    L+  
Sbjct: 185 RKPIKASSKGVNRKKKALKVVKPNRHETLENTWNMITEEGKSTPLTCHYRKTSMSGLNAG 244

Query: 252 RDEEEETAIGRNSSSPPKVTKSETTFNGQELKLRQSA----TEGRVRKMPSLTEEELNRR 288
            D +            P + K+ET         RQS+    +  +++K  S + EELNRR
Sbjct: 245 GDVK------------PVLRKAETF--RDVTNYRQSSPTVTSPVKMKKEMSPSREELNRR 304

BLAST of Lsi04G012810 vs. TAIR 10
Match: AT1G11230.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-08
Identity = 85/310 (27.42%), Postives = 134/310 (43.23%), Query Frame = 0

Query: 15  LKLLLLSSALL-SMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFIIISI 74
           +K +L+S+ ++ +M++ LK ++PV     LS    +WSS + WLKPPYL++ VN +I  I
Sbjct: 7   IKAVLISTGIITAMSMFLKVFLPVTLYFSLS-FSTLWSSFLPWLKPPYLFVFVNVMITII 66

Query: 75  LASSKLHKDLVD---------------------------PPPTTMPKPSADSQMDFTVSR 134
           +ASS+ ++ + D                           PP   +     D   DF    
Sbjct: 67  IASSRYYRSIGDHDGKDEKNLHGRGYYRIQTEPIVNQTSPPRLEVKDMDLDVDFDF---M 126

Query: 135 AVVSDVLMKRGRGANENFAADVSE-----DE--GKSEYSRTTYDKKPPISSKVGNRKAAK 194
           A +    ++R     E    ++SE     DE     E       +KP +S++  +RK  K
Sbjct: 127 ATIQPPEVERSEVVYEEKEEEISELINGGDEFVVLEESENLPPVEKPLVSARFEHRKMVK 186

Query: 195 AAATPAGTTTRWSRMN-GHDTLDDAWKAIT-EGESAPFSRQLDKSDTWELHTKRDEEEET 254
              TP G   R   +   +   D+ WK I+ EG S P S           H +R +    
Sbjct: 187 --VTPKGDDIRKKALKVVNPKRDNKWKTISEEGTSRPLSTS---------HYQRPDIFGL 246

Query: 255 AIGRNSSSPPKVTKSETTFNGQELKLRQSATEGRVRKMPSLTEEELNRRVEAFIEKFKEE 284
             G +S    +  +  T +  Q         +     +PSL  E+LNRR+EAFI+K KEE
Sbjct: 247 GAGGDSLRKSETFRDVTNYYHQSSLTVTPPVKMEKEMLPSL--EDLNRRIEAFIKKVKEE 299

BLAST of Lsi04G012810 vs. TAIR 10
Match: AT1G11210.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 53.1 bits (126), Expect = 4.3e-07
Identity = 81/309 (26.21%), Postives = 136/309 (44.01%), Query Frame = 0

Query: 15  LKLLLLSSALLSMAVILKFYVPVVAGILLSDVPAVWSSIVFWLKPPYLYLLVNFIIISIL 74
           +K +L+S+ +++ A+ LK  VPV      S  P + SS + WLKPPYLY++ N III + 
Sbjct: 5   MKAVLISTGVVATAMHLKVIVPVAMD--FSQNPIILSSFLTWLKPPYLYVITNVIIIVVG 64

Query: 75  ASSKL-------------------------HKDLVDPPPTTMPKPSADSQMDFTVSRAVV 134
            S ++                         H+ +V   P      + D+  DF     V+
Sbjct: 65  VSYRITTVSSHVDGKDYEASYSGDNKFQTDHQQIVQEAPLRRRTETKDA--DFGFIGKVL 124

Query: 135 SDVLMKRGRGANENFAADVSEDEGK-----SEYSRTTYDKKPPISSKVGNRKAAKAAATP 194
             V         +   A V E+E K     S+       +KP +++++G +K      TP
Sbjct: 125 QIVKEPEVVYEEKERPATVEEEEKKCIIVVSKSENQPPVEKPLVTARIGQKKPV-VKTTP 184

Query: 195 AGTTT----RWSRMNGHDTLDDAWKAITEGESA--PFSRQLDKSDTWELHTKRDEEEETA 254
           A   +    R ++   ++TL++ WK I EG  +  P +    + DT+ L    +E +++ 
Sbjct: 185 AERNSMRALRVAKPKRNETLENTWKMIMEGNKSTLPLTSYYKRPDTFGL---GEETKQSG 244

Query: 255 IGRNSSSPPKVTKSETTFNGQELKLRQSATEGRVRKMPSLTEEELNRRVEAFIEKFKEE- 284
           + + S      T S+ T   Q L           +   S + +ELNR+VEAFI+K  +E 
Sbjct: 245 VLKKSE-----TFSDRTNCYQSLPPPPPPLVKVKKVKVSRSRDELNRKVEAFIKKCNDER 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1IHJ98.9e-9354.57uncharacterized protein LOC111475963 OS=Cucurbita maxima OX=3661 GN=LOC111475963... [more]
A0A6J1F7891.5e-9267.65uncharacterized protein LOC111442999 OS=Cucurbita moschata OX=3662 GN=LOC1114429... [more]
A0A2P5B6Z89.0e-4538.48DUF4408 domain-containing protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14... [more]
W9RGE15.9e-4439.64DUF4408 domain-containing protein OS=Morus notabilis OX=981085 GN=L484_018748 PE... [more]
A0A498KDZ01.0e-4338.57DUF4408 domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_004335 PE=... [more]
Match NameE-valueIdentityDescription
XP_023535113.18.8e-10369.79uncharacterized protein LOC111796630 [Cucurbita pepo subsp. pepo][more]
KAG6591991.14.8e-10168.67hypothetical protein SDJN03_14337, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022975735.11.8e-9254.57uncharacterized protein LOC111475963 [Cucurbita maxima][more]
XP_022936351.13.1e-9267.65uncharacterized protein LOC111442999 [Cucurbita moschata][more]
KAF3453608.11.9e-4439.15hypothetical protein FNV43_RR04048 [Rhamnella rubrinervis][more]
Match NameE-valueIdentityDescription
AT1G61260.12.1e-2531.25Protein of unknown function (DUF761) [more]
AT5G54300.11.9e-2333.75Protein of unknown function (DUF761) [more]
AT1G11220.16.2e-1427.73Protein of unknown function (DUF761) [more]
AT1G11230.11.8e-0827.42Protein of unknown function (DUF761) [more]
AT1G11210.14.3e-0726.21Protein of unknown function (DUF761) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025520Domain of unknown function DUF4408PFAMPF14364DUF4408coord: 47..79
e-value: 2.1E-14
score: 53.0
IPR008480Protein of unknown function DUF761, plantPFAMPF05553DUF761coord: 260..294
e-value: 3.7E-13
score: 48.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..244
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..257
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..141
NoneNo IPR availablePANTHERPTHR33098:SF65COTTON FIBER EXPRESSED PROTEIN 1-LIKE PROTEINcoord: 136..294
NoneNo IPR availablePANTHERPTHR33098:SF65COTTON FIBER EXPRESSED PROTEIN 1-LIKE PROTEINcoord: 6..100
NoneNo IPR availablePANTHERPTHR33098COTTON FIBER (DUF761)coord: 6..100
NoneNo IPR availablePANTHERPTHR33098COTTON FIBER (DUF761)coord: 136..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G012810.1Lsi04G012810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane