Lsi04G012760 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G012760
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionvesicle-associated protein 4-1
Locationchr04: 20136103 .. 20139840 (+)
RNA-Seq ExpressionLsi04G012760
SyntenyLsi04G012760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACTGATTTTTAAGATAGCAAGAAAAGGCGCGTGCAACTCACGCGTCCTATAACCGCGTGTGATAACTATTCCAAATAATTCCATCTCATCGGAAAAAAAATATTACAACTCAGAGCAGCACGGATTCGTAATAATTGCACGAACGGCCCTTTGGGCTGTCAAGACGGATTCTCCGCGCGCCACGTGTCCAACCCTGCACGGACATCAAAACCAAATTCCGTCGTATTTACATTCGCCTCTCGAATCCGCTTTCTCTCCGCCACCGTCGTCTCCTCCTCCTCCGTCAGATTGAAATTGGCGAATTTCAGTTTTGGCTCGAAAAATTTAATGTGATAGTGTTAATGACCATGGCCGTTGCCGACCGTCACAAGCCACACGCCGACGTGAAGCTCTTCAGGCTGTGTCCGATTTGGCAATCAGGGAATACTAATTCGTCTTCTTCCTCGTCGACTCAGAACCTTCACCATAGTGGCCATGGCCCTAGCGACAGAAACTCAACGGCGTCGAATAACATGAAAGCTTCATCTTCTTCCAAGACGGTGTCCTCTGTGGCGAAGTCGCTGTTGCCTCCTAGGCGTAGGCTCCGTCTTGATCCTAACAATTCTTTGTTCTTTCCTTGTAAGTTGTTGTTCTATCTGTCTCTCTCTCTTCTCCGTTGTTGATGTTTAGATTTGTGAATGGAATGGAGAGCTTTTGCTGTTTTTGAATTATAGTGTTTAATTAGAATTCGAAATTGGATGTTTCCAACTTCTAGAGAGTGGAAGAAGCTAGCACGTTTTTAATCTTCAATCTGGATAATGCGAGCCGAAACTATACTCATTTGCTGTTGGATTATAATATTTAGCTTTTCTGAATTGAATTAAATAATGGTTCTAGTCTTCGGATTTTCCTGCTTCCATTTGCGAGTTGAAGTTCATATTTACTTGAAGAAGGGGAAGTGTACGGATTTATTGTTTAGAATTTGCATTCATCTTTAACTCTGTATTGTCTGATGATTTTTTTTCCCCTGTAGATGAACCCGGAAAGCAGGTAAAGAGCGCCATCAGGTTGAAGAATACGAGCAGATCCCATGTAGCTTTTAAGGTATTACTTTAATTATTTTCCATTGCAGGCCTACTTTCACCTTTTGTTCTTTCAATTATTCTTATATTGATTTCTTACTTCTTAAGTCCTTACAAGCCAAGCGTACGGCAAACGCTTTACTGAAACATGAATGCATGTGGTTCTGTTCTTCCATGTACTTTTCTGAAATCCCATGCATGTTAATTATTATTTTTAAGGGTAATTAATGTTTTTGGGAACTTATTAAAAGAATGAAAAATTTGCATTTAAATTGTTTTCAGCTTCTTGTAAAGTTGTTGAGCTGAGGCAATCCAGTTCGTCGAAGGTTAAATCCATTAATGACGGAGATATTATATCATCTTTTTCCTTATTTGGCGATTTAACACTTTCTTCGAAGAATTATTATAAGTTTCAATTAATTCAACAGCTAGATCATTGGATTTATTGTATTATTAATCTAGGTTTTGGTTTTCCTAACGTAAATTGACATCACCAGCAAGGCAGGTAAAGGCTGGTGAACTGCATACTTGTTTGAATGTCATTTCAAACCAATGTGAACTGTTCATTTCTATGATTAGTTAGTTAAAATGTGAGTATATATATCTTTCTAAATTGTCTCTGCATCTTTTTTCAGTTTCAAACTACAGCCCCGAAGAGCTGTTATATGCGGCCCCCTGGTGGCATTCTTGCTCCTGGAGAAAATTTTATTGCTACTGGTAATGTTTCCATTGTCATGATCTTTTCTCTTTTGTCCTGTCAAACATTTTGTTTTTTGTGGTAAATGATAGTCAATTCATTAGTGTATTTTCATTTCTCCTTTTTCATGGTTTCCTTGCTTACGTAGTCAATATCAACACAAGTATTAATTTGCTTCATCTCTTAAACAGTCTTCAGATTTGTGGAGCACCCCGAGAACAATGAAAAGCCAATGGACCAGAAGAGCAAAATTAAGTTTAAGATCATGAGCCTTAAAGTGAAAGGAGGAACAGAATACGCACCCGAGCTGGTGAGTGTCAGTTTAAATTGGGTTTCCAACTTTTCATGCCTCTTTCTATGCTATTTTGGTTTGTTAAACATAAACCTCTCTGGCTCTTCATTATGAAAGATAGCCAATGTCTTGCCATTTTTGTTATTTCTTGTGTATTATTGACAGCTTAAGCTTTCCATTGTGCTACCTCTGTGCACTTATTTTAGTTACCTCCATGTTGTCAATGTTGTTTAGTGTGATTTCTTGTCAAACAGCATCTCCCGTTTGTAGGTTCTCTTGCCATCTAATCAGCGTACTAATTTGTTGTGGACTTTTCTACGGTTAACTGAAATAAGACAATAAGTTTGTTTTACATTATTTGACGTTGTACAGAATTACCTTATGACCACACACACCTCAAGCAATCAACTCGGTTCACAGTTGTGTTCAACCACTAAATTAATGATACATTGTGAAAGCTTGAATTTATTTCCAAATTTGTTGATGGCATGTGTTTATGTTCTGCAAAATTTCAGTTTGAAGAACAGAAGGACCAAGTAACAGTTGAGCGGATTTTGCGGGTCGTATTTGTAGATCCAGAGCGACCTAGTGCAGTAAGTGAATCAATGAATACAAGGCTTATTCTTGTCTATATTGGAGAGAGAGAGAGGTCCAATTTAACTGCATTTTGATATTTTAGGCTCTCGAAAAGCTGAAACGTCAATTGGCCGAAGCCGAGGCTGCACTTGAAGCACGCAAGAAACCTCCTGTAGAAACAGGTCATCAGGTTGTAGGGGGTGAAGGTCTTGTGATAGATGAATGGGTACGCTGATCTTGATCTGATGGGTGCATTTTTGTATCCAATAATGTTCAGTATTAAGTTCGTCTTACGTAGATCTTTTAATCCTCTGATCTTCATTTTTGGATTGTTCAGAAAGAACGGAGGGAGAAATACCTTGCTCGTCAACAAGTCGGCGCAGTAGACTCTGTGTAAAGTTAGATTTACTGTTGCACTACACTCTTTTCGGTGGTAAAATCTGTACAGGTATTGTCATCTTCAAGCCCTGGATATTTGGAAGATGAAAGGAGTTGTTAGTTGGAAAGTTCATCTGACCGAAAAAAAAAAAAATCCGATGTCAATGTGACTCATGAAATGACCAGTACGAATGCTGATATCAACTTGAAGAACTTGATCGAAAGTCGAATGCTCATTGTAGATAATGAATTTTGCTTGTGAAAATAAATGTACTACACATCAATCATCTCAGCTTATGATCATAAGATAAATATAATATGGTTTCTTGATTGGAATGGAATCAATAGAACCTTTGGATAGATAAGTTGTGTACTATGGTGCTTAAGGGATGCTGTATTTCCAAAAATTCCATAATTTAGAGCTTAAAATTGGGTGCTGCTTCTATTATTCAATCAAGCAGGGCCAGGATATAAGATGTATGAATGGCTTATGCCATTTTCTAGCTGCAACCAAGCCATCCTTTTAGGTTTCAAATTTCGTTAATGGAAGAAAGTGAAACTTCTTTCCTTTGTTTATCTGTTTGCCTACATCTTTTTTTTTTTCTTGGTTCTGTTTTGGAGCTTTTATGGTGTGTTTAATTCATAAATCATACTAGTTTTGTAACTACTATGTATTTTTATCCTTTGTAATGTACAAAAGCTCTATTTGTATTTGTTAATGAACTCTTCT

mRNA sequence

CAAAACTGATTTTTAAGATAGCAAGAAAAGGCGCGTGCAACTCACGCGTCCTATAACCGCGTGTGATAACTATTCCAAATAATTCCATCTCATCGGAAAAAAAATATTACAACTCAGAGCAGCACGGATTCGTAATAATTGCACGAACGGCCCTTTGGGCTGTCAAGACGGATTCTCCGCGCGCCACGTGTCCAACCCTGCACGGACATCAAAACCAAATTCCGTCGTATTTACATTCGCCTCTCGAATCCGCTTTCTCTCCGCCACCGTCGTCTCCTCCTCCTCCGTCAGATTGAAATTGGCGAATTTCAGTTTTGGCTCGAAAAATTTAATGTGATAGTGTTAATGACCATGGCCGTTGCCGACCGTCACAAGCCACACGCCGACGTGAAGCTCTTCAGGCTGTGTCCGATTTGGCAATCAGGGAATACTAATTCGTCTTCTTCCTCGTCGACTCAGAACCTTCACCATAGTGGCCATGGCCCTAGCGACAGAAACTCAACGGCGTCGAATAACATGAAAGCTTCATCTTCTTCCAAGACGGTGTCCTCTGTGGCGAAGTCGCTGTTGCCTCCTAGGCGTAGGCTCCGTCTTGATCCTAACAATTCTTTGTTCTTTCCTTATGAACCCGGAAAGCAGGTAAAGAGCGCCATCAGGTTGAAGAATACGAGCAGATCCCATGTAGCTTTTAAGTTTCAAACTACAGCCCCGAAGAGCTGTTATATGCGGCCCCCTGGTGGCATTCTTGCTCCTGGAGAAAATTTTATTGCTACTGTCTTCAGATTTGTGGAGCACCCCGAGAACAATGAAAAGCCAATGGACCAGAAGAGCAAAATTAAGTTTAAGATCATGAGCCTTAAAGTGAAAGGAGGAACAGAATACGCACCCGAGCTGTTTGAAGAACAGAAGGACCAAGTAACAGTTGAGCGGATTTTGCGGGTCGTATTTGTAGATCCAGAGCGACCTAGTGCAGCTCTCGAAAAGCTGAAACGTCAATTGGCCGAAGCCGAGGCTGCACTTGAAGCACGCAAGAAACCTCCTGTAGAAACAGGTCATCAGGTTGTAGGGGGTGAAGGTCTTGTGATAGATGAATGGAAAGAACGGAGGGAGAAATACCTTGCTCGTCAACAAGTCGGCGCAGTAGACTCTGTGTAAAGTTAGATTTACTGTTGCACTACACTCTTTTCGGTGGTAAAATCTGTACAGGTATTGTCATCTTCAAGCCCTGGATATTTGGAAGATGAAAGGAGTTGTTAGTTGGAAAGTTCATCTGACCGAAAAAAAAAAAAATCCGATGTCAATGTGACTCATGAAATGACCAGTACGAATGCTGATATCAACTTGAAGAACTTGATCGAAAGTCGAATGCTCATTGTAGATAATGAATTTTGCTTGTGAAAATAAATGTACTACACATCAATCATCTCAGCTTATGATCATAAGATAAATATAATATGGTTTCTTGATTGGAATGGAATCAATAGAACCTTTGGATAGATAAGTTGTGTACTATGGTGCTTAAGGGATGCTGTATTTCCAAAAATTCCATAATTTAGAGCTTAAAATTGGGTGCTGCTTCTATTATTCAATCAAGCAGGGCCAGGATATAAGATGTATGAATGGCTTATGCCATTTTCTAGCTGCAACCAAGCCATCCTTTTAGGTTTCAAATTTCGTTAATGGAAGAAAGTGAAACTTCTTTCCTTTGTTTATCTGTTTGCCTACATCTTTTTTTTTTTCTTGGTTCTGTTTTGGAGCTTTTATGGTGTGTTTAATTCATAAATCATACTAGTTTTGTAACTACTATGTATTTTTATCCTTTGTAATGTACAAAAGCTCTATTTGTATTTGTTAATGAACTCTTCT

Coding sequence (CDS)

ATGACCATGGCCGTTGCCGACCGTCACAAGCCACACGCCGACGTGAAGCTCTTCAGGCTGTGTCCGATTTGGCAATCAGGGAATACTAATTCGTCTTCTTCCTCGTCGACTCAGAACCTTCACCATAGTGGCCATGGCCCTAGCGACAGAAACTCAACGGCGTCGAATAACATGAAAGCTTCATCTTCTTCCAAGACGGTGTCCTCTGTGGCGAAGTCGCTGTTGCCTCCTAGGCGTAGGCTCCGTCTTGATCCTAACAATTCTTTGTTCTTTCCTTATGAACCCGGAAAGCAGGTAAAGAGCGCCATCAGGTTGAAGAATACGAGCAGATCCCATGTAGCTTTTAAGTTTCAAACTACAGCCCCGAAGAGCTGTTATATGCGGCCCCCTGGTGGCATTCTTGCTCCTGGAGAAAATTTTATTGCTACTGTCTTCAGATTTGTGGAGCACCCCGAGAACAATGAAAAGCCAATGGACCAGAAGAGCAAAATTAAGTTTAAGATCATGAGCCTTAAAGTGAAAGGAGGAACAGAATACGCACCCGAGCTGTTTGAAGAACAGAAGGACCAAGTAACAGTTGAGCGGATTTTGCGGGTCGTATTTGTAGATCCAGAGCGACCTAGTGCAGCTCTCGAAAAGCTGAAACGTCAATTGGCCGAAGCCGAGGCTGCACTTGAAGCACGCAAGAAACCTCCTGTAGAAACAGGTCATCAGGTTGTAGGGGGTGAAGGTCTTGTGATAGATGAATGGAAAGAACGGAGGGAGAAATACCTTGCTCGTCAACAAGTCGGCGCAGTAGACTCTGTGTAA

Protein sequence

MTMAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASSSSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGGEGLVIDEWKERREKYLARQQVGAVDSV
Homology
BLAST of Lsi04G012760 vs. ExPASy Swiss-Prot
Match: Q1ECE0 (Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 5.2e-97
Identity = 191/271 (70.48%), Postives = 223/271 (82.29%), Query Frame = 0

Query: 3   MAVADRHKPHADVK--LFRLCPIWQSGNTNSSSSSSTQNLHH-SGHGPSDRNSTASNNMK 62
           M + DR  P  + K  LFRLCP WQ  +T SSSS+   N ++ S HG  +RN+  S    
Sbjct: 1   MPIGDRQNPSVEKKKNLFRLCPFWQRRSTTSSSSTQNPNQNYRSRHG--NRNTDIS---A 60

Query: 63  ASSSSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQT 122
            S    T+SSVA+SLLP RRRLRLDP++ L+FPYEPGKQV+SAI+LKNTS+SH AFKFQT
Sbjct: 61  VSKPPLTMSSVARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQT 120

Query: 123 TAPKSCYMRPPGGILAPGENFIATVFRFVEHPENNEK-PMDQKSKIKFKIMSLKVKGGTE 182
           TAPKSCYMRPPGG+LAPGE+  ATVF+FVEHPENNEK P++QKSK+KFKIMSLKVK G E
Sbjct: 121 TAPKSCYMRPPGGVLAPGESVFATVFKFVEHPENNEKQPLNQKSKVKFKIMSLKVKPGVE 180

Query: 183 YAPELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQ 242
           Y PELF+EQKDQV VE++LRV+F+D +RPSAALEKLKRQL EAEAA+EARKKPP ETG +
Sbjct: 181 YVPELFDEQKDQVAVEQVLRVIFIDADRPSAALEKLKRQLDEAEAAVEARKKPPPETGPR 240

Query: 243 VVGGEGLVIDEWKERREKYLARQQVGAVDSV 270
           VV GEGLVIDEWKERREKYLARQQV +VDS+
Sbjct: 241 VV-GEGLVIDEWKERREKYLARQQVESVDSL 265

BLAST of Lsi04G012760 vs. ExPASy Swiss-Prot
Match: Q8VYN2 (Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.0e-84
Identity = 166/254 (65.35%), Postives = 201/254 (79.13%), Query Frame = 0

Query: 26  SGNTNSSSSSSTQNLHHSGH----------GPSDRNSTASNNMKASSSSKTVSSVAKSLL 85
           SG ++SS+SS   N H   H          G +  +   S   +  + S +VSSVAKS L
Sbjct: 43  SGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFL 102

Query: 86  PPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILA 145
           P +RRL+LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTTAPKSC+MRPPG ILA
Sbjct: 103 PTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILA 162

Query: 146 PGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPELFEEQKDQVTVER 205
           PGE  IATVF+FVE PENNEKPMDQ+S++KFKIMSLKVKG  +Y PELF+EQKD V+ E+
Sbjct: 163 PGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQ 222

Query: 206 ILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGGEGLVIDEWKERRE 265
           ILRV+F+DPER + ALEKLKRQLAEA+AA+EARKKPP ETG +++ GEGLVIDEWKERRE
Sbjct: 223 ILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEETGPKMI-GEGLVIDEWKERRE 282

Query: 266 KYLARQQVGAVDSV 270
           +YLA+QQ    DSV
Sbjct: 283 RYLAQQQGEGADSV 295

BLAST of Lsi04G012760 vs. ExPASy Swiss-Prot
Match: Q8LPQ7 (Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.5e-75
Identity = 154/252 (61.11%), Postives = 195/252 (77.38%), Query Frame = 0

Query: 29  TNSSSSSST--------QNLHHSGHGPSDRNSTASNNMKASSSSKTVSSVAKSLLPPRRR 88
           T+SSS +S+        Q+ H   HGP        N+ +++++  ++SSVA+SLLP +RR
Sbjct: 40  TSSSSVTSSHLNQNYIHQSRHFQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRR 99

Query: 89  LRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPGENF 148
           L+LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTT PKSC+MRP G ILAPGE  
Sbjct: 100 LKLDPSAKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEI 159

Query: 149 IATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPELFEEQKDQVTVERILRVV 208
           IATVF+FVE PENNEKPM+QKS +KFKIMSLK+K  T+Y PELFEEQKD V+ E+++RVV
Sbjct: 160 IATVFKFVEPPENNEKPMEQKSGVKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVV 219

Query: 209 FVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVG----GEGLVIDEWKERREK 268
           F+DPE P++ +EKLK QLAEA+AA EARKK        +VG    GEGLVIDEWK+RRE+
Sbjct: 220 FLDPENPNSMMEKLKSQLAEADAADEARKK----ASEGIVGPKPIGEGLVIDEWKQRRER 279

BLAST of Lsi04G012760 vs. ExPASy Swiss-Prot
Match: Q9SYC9 (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.2e-07
Identity = 36/127 (28.35%), Postives = 65/127 (51.18%), Query Frame = 0

Query: 91  FPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPGENF-IATVFRFVE 150
           FP E  KQ   ++ L N + ++VAFK QTT PK   ++P  G++ P  +  +  V + ++
Sbjct: 16  FPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPRSSCEVLVVMQALK 75

Query: 151 HPENNEKPMDQKSKIKFKIMSLKVKGGT---EYAPELFEEQKDQVTVERILRVVFVDPER 210
                E P D++ K K       V+ GT   E   E+F ++      E I ++++V P +
Sbjct: 76  -----EAPADRQCKDKLLFQCKVVEPGTMDKEVTSEMFSKEAGHRVEETIFKIIYVAPPQ 135

Query: 211 PSAALEK 214
           P + +++
Sbjct: 136 PQSPVQE 137

BLAST of Lsi04G012760 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.5e-06
Identity = 39/120 (32.50%), Postives = 55/120 (45.83%), Query Frame = 0

Query: 88  SLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPGENFIATVFRF 147
           +L F  +  KQ    ++L NT+  +VAFK +TT+PK   +RP  G++AP      TV   
Sbjct: 12  TLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAPKSTCEFTVIM- 71

Query: 148 VEHPENNEKPMDQKSKIKFKIMSLKVKGGT---EYAPELFEEQKDQVTVERILRVVFVDP 205
                  E P D   K KF I S  V   T   +    +F + + +   E  LRV  V P
Sbjct: 72  ---QAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKLRVTLVPP 127

BLAST of Lsi04G012760 vs. ExPASy TrEMBL
Match: A0A5A7T1W8 (Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20G00390 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.0e-135
Identity = 252/267 (94.38%), Postives = 257/267 (96.25%), Query Frame = 0

Query: 3   MAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASS 62
           MAVADR K HADVKLFRLCPIWQSGNTNSSSSSST NLHH+ HGPSDR     NNMKASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 63  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAP 122
           SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 123 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 182
           KSCYMRPPGGILAPGENFIATVFRFVEHPENNE+PMDQKSKIKFKIMSLKVKGGTEYAPE
Sbjct: 121 KSCYMRPPGGILAPGENFIATVFRFVEHPENNERPMDQKSKIKFKIMSLKVKGGTEYAPE 180

Query: 183 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGG 242
           LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETG QV+GG
Sbjct: 181 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGPQVIGG 240

Query: 243 EGLVIDEWKERREKYLARQQVGAVDSV 270
           EGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 EGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Lsi04G012760 vs. ExPASy TrEMBL
Match: A0A1S3BZ62 (vesicle-associated protein 4-1 OS=Cucumis melo OX=3656 GN=LOC103494990 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.0e-135
Identity = 252/267 (94.38%), Postives = 257/267 (96.25%), Query Frame = 0

Query: 3   MAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASS 62
           MAVADR K HADVKLFRLCPIWQSGNTNSSSSSST NLHH+ HGPSDR     NNMKASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 63  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAP 122
           SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 123 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 182
           KSCYMRPPGGILAPGENFIATVFRFVEHPENNE+PMDQKSKIKFKIMSLKVKGGTEYAPE
Sbjct: 121 KSCYMRPPGGILAPGENFIATVFRFVEHPENNERPMDQKSKIKFKIMSLKVKGGTEYAPE 180

Query: 183 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGG 242
           LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETG QV+GG
Sbjct: 181 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGPQVIGG 240

Query: 243 EGLVIDEWKERREKYLARQQVGAVDSV 270
           EGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 EGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Lsi04G012760 vs. ExPASy TrEMBL
Match: A0A5D3BAA2 (Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001120 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.1e-134
Identity = 251/267 (94.01%), Postives = 257/267 (96.25%), Query Frame = 0

Query: 3   MAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASS 62
           MAVADR K HADVKLFRLCPIWQSGNTNSSSSSST NLHH+ HGPSDR     NNMKASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 63  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAP 122
           SSKTVSSVAKSLLPPRR+LRLDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRKLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 123 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 182
           KSCYMRPPGGILAPGENFIATVFRFVEHPENNE+PMDQKSKIKFKIMSLKVKGGTEYAPE
Sbjct: 121 KSCYMRPPGGILAPGENFIATVFRFVEHPENNERPMDQKSKIKFKIMSLKVKGGTEYAPE 180

Query: 183 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGG 242
           LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETG QV+GG
Sbjct: 181 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGPQVIGG 240

Query: 243 EGLVIDEWKERREKYLARQQVGAVDSV 270
           EGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 EGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Lsi04G012760 vs. ExPASy TrEMBL
Match: A0A0A0LFW4 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G851700 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.3e-131
Identity = 246/267 (92.13%), Postives = 251/267 (94.01%), Query Frame = 0

Query: 3   MAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASS 62
           MAVA RHK H DVKLFRLCPIWQSGNT+SSSSSST NLHH  H PSD       N+KASS
Sbjct: 1   MAVAHRHKSHPDVKLFRLCPIWQSGNTHSSSSSSTHNLHHPTHPPSD------TNLKASS 60

Query: 63  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAP 122
           SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 123 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 182
           KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE
Sbjct: 121 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 180

Query: 183 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGG 242
           LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEA LEARKKPPVETG QV+GG
Sbjct: 181 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAVLEARKKPPVETGPQVIGG 240

Query: 243 EGLVIDEWKERREKYLARQQVGAVDSV 270
           EGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 EGLVIDEWKERREKYLARQQVGALDSV 261

BLAST of Lsi04G012760 vs. ExPASy TrEMBL
Match: A0A6J1INV3 (vesicle-associated protein 4-1-like OS=Cucurbita maxima OX=3661 GN=LOC111476917 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.8e-130
Identity = 244/269 (90.71%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 1   MTMAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKA 60
           MTM VADRHKPHADVK FRLC IWQSGNT SSSS STQN+HHS +GP DRNSTASNN+KA
Sbjct: 1   MTMTVADRHKPHADVKFFRLCSIWQSGNT-SSSSLSTQNIHHSRNGPKDRNSTASNNVKA 60

Query: 61  SSSSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTT 120
           SSSSKTVS VAKSLLPPRRRL LDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTT
Sbjct: 61  SSSSKTVSFVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTT 120

Query: 121 APKSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYA 180
           APKSCYMRPPGGILAP E FIATVFRFVEHPENNEKP D KSKIKFKIMSLKVKGGTEYA
Sbjct: 121 APKSCYMRPPGGILAPEEIFIATVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYA 180

Query: 181 PELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVV 240
           PELF+EQKDQV VERILRV+FVDPERPSAALE+LKRQLAEAEAALEARKKPP E+G QVV
Sbjct: 181 PELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAESGPQVV 240

Query: 241 GGEGLVIDEWKERREKYLARQQVGAVDSV 270
           GGEGLVIDEWKERREKYLARQQVG VDSV
Sbjct: 241 GGEGLVIDEWKERREKYLARQQVGTVDSV 268

BLAST of Lsi04G012760 vs. NCBI nr
Match: XP_038900245.1 (vesicle-associated protein 4-1 [Benincasa hispida])

HSP 1 Score: 517.3 bits (1331), Expect = 7.9e-143
Identity = 263/269 (97.77%), Postives = 266/269 (98.88%), Query Frame = 0

Query: 1   MTMAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKA 60
           MTMAVADRHKPHAD+KLFRLCPIWQSGNTNSSSSSSTQNLHHS HGPSDRNST SNNMKA
Sbjct: 1   MTMAVADRHKPHADIKLFRLCPIWQSGNTNSSSSSSTQNLHHSSHGPSDRNSTVSNNMKA 60

Query: 61  SSSSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTT 120
           S+SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTT
Sbjct: 61  SASSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTT 120

Query: 121 APKSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYA 180
           APKSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYA
Sbjct: 121 APKSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYA 180

Query: 181 PELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVV 240
           PELFEEQKDQVTVERILRVVFVDPERPSAAL+KLKRQLAEAEAALEARKKPPVETG QVV
Sbjct: 181 PELFEEQKDQVTVERILRVVFVDPERPSAALDKLKRQLAEAEAALEARKKPPVETGPQVV 240

Query: 241 GGEGLVIDEWKERREKYLARQQVGAVDSV 270
           GGEGLVIDEWKERREKYLARQQVGAVDSV
Sbjct: 241 GGEGLVIDEWKERREKYLARQQVGAVDSV 269

BLAST of Lsi04G012760 vs. NCBI nr
Match: XP_008454625.1 (PREDICTED: vesicle-associated protein 4-1 [Cucumis melo] >KAA0036758.1 vesicle-associated protein 4-1 [Cucumis melo var. makuwa])

HSP 1 Score: 490.3 bits (1261), Expect = 1.0e-134
Identity = 252/267 (94.38%), Postives = 257/267 (96.25%), Query Frame = 0

Query: 3   MAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASS 62
           MAVADR K HADVKLFRLCPIWQSGNTNSSSSSST NLHH+ HGPSDR     NNMKASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 63  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAP 122
           SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 123 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 182
           KSCYMRPPGGILAPGENFIATVFRFVEHPENNE+PMDQKSKIKFKIMSLKVKGGTEYAPE
Sbjct: 121 KSCYMRPPGGILAPGENFIATVFRFVEHPENNERPMDQKSKIKFKIMSLKVKGGTEYAPE 180

Query: 183 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGG 242
           LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETG QV+GG
Sbjct: 181 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGPQVIGG 240

Query: 243 EGLVIDEWKERREKYLARQQVGAVDSV 270
           EGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 EGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Lsi04G012760 vs. NCBI nr
Match: TYJ95826.1 (vesicle-associated protein 4-1 [Cucumis melo var. makuwa])

HSP 1 Score: 489.2 bits (1258), Expect = 2.3e-134
Identity = 251/267 (94.01%), Postives = 257/267 (96.25%), Query Frame = 0

Query: 3   MAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASS 62
           MAVADR K HADVKLFRLCPIWQSGNTNSSSSSST NLHH+ HGPSDR     NNMKASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 63  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAP 122
           SSKTVSSVAKSLLPPRR+LRLDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRKLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 123 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 182
           KSCYMRPPGGILAPGENFIATVFRFVEHPENNE+PMDQKSKIKFKIMSLKVKGGTEYAPE
Sbjct: 121 KSCYMRPPGGILAPGENFIATVFRFVEHPENNERPMDQKSKIKFKIMSLKVKGGTEYAPE 180

Query: 183 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGG 242
           LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETG QV+GG
Sbjct: 181 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGPQVIGG 240

Query: 243 EGLVIDEWKERREKYLARQQVGAVDSV 270
           EGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 EGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Lsi04G012760 vs. NCBI nr
Match: XP_011652378.1 (vesicle-associated protein 4-1 [Cucumis sativus] >KGN59869.1 hypothetical protein Csa_001849 [Cucumis sativus])

HSP 1 Score: 477.6 bits (1228), Expect = 6.9e-131
Identity = 246/267 (92.13%), Postives = 251/267 (94.01%), Query Frame = 0

Query: 3   MAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKASS 62
           MAVA RHK H DVKLFRLCPIWQSGNT+SSSSSST NLHH  H PSD       N+KASS
Sbjct: 1   MAVAHRHKSHPDVKLFRLCPIWQSGNTHSSSSSSTHNLHHPTHPPSD------TNLKASS 60

Query: 63  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAP 122
           SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 123 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 182
           KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE
Sbjct: 121 KSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPE 180

Query: 183 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGG 242
           LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEA LEARKKPPVETG QV+GG
Sbjct: 181 LFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAVLEARKKPPVETGPQVIGG 240

Query: 243 EGLVIDEWKERREKYLARQQVGAVDSV 270
           EGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 EGLVIDEWKERREKYLARQQVGALDSV 261

BLAST of Lsi04G012760 vs. NCBI nr
Match: XP_022976559.1 (vesicle-associated protein 4-1-like [Cucurbita maxima])

HSP 1 Score: 473.8 bits (1218), Expect = 1.0e-129
Identity = 244/269 (90.71%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 1   MTMAVADRHKPHADVKLFRLCPIWQSGNTNSSSSSSTQNLHHSGHGPSDRNSTASNNMKA 60
           MTM VADRHKPHADVK FRLC IWQSGNT SSSS STQN+HHS +GP DRNSTASNN+KA
Sbjct: 1   MTMTVADRHKPHADVKFFRLCSIWQSGNT-SSSSLSTQNIHHSRNGPKDRNSTASNNVKA 60

Query: 61  SSSSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTT 120
           SSSSKTVS VAKSLLPPRRRL LDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTT
Sbjct: 61  SSSSKTVSFVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTT 120

Query: 121 APKSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYA 180
           APKSCYMRPPGGILAP E FIATVFRFVEHPENNEKP D KSKIKFKIMSLKVKGGTEYA
Sbjct: 121 APKSCYMRPPGGILAPEEIFIATVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYA 180

Query: 181 PELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVV 240
           PELF+EQKDQV VERILRV+FVDPERPSAALE+LKRQLAEAEAALEARKKPP E+G QVV
Sbjct: 181 PELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAESGPQVV 240

Query: 241 GGEGLVIDEWKERREKYLARQQVGAVDSV 270
           GGEGLVIDEWKERREKYLARQQVG VDSV
Sbjct: 241 GGEGLVIDEWKERREKYLARQQVGTVDSV 268

BLAST of Lsi04G012760 vs. TAIR 10
Match: AT5G54110.1 (membrane-associated mannitol-induced )

HSP 1 Score: 355.5 bits (911), Expect = 3.7e-98
Identity = 191/271 (70.48%), Postives = 223/271 (82.29%), Query Frame = 0

Query: 3   MAVADRHKPHADVK--LFRLCPIWQSGNTNSSSSSSTQNLHH-SGHGPSDRNSTASNNMK 62
           M + DR  P  + K  LFRLCP WQ  +T SSSS+   N ++ S HG  +RN+  S    
Sbjct: 1   MPIGDRQNPSVEKKKNLFRLCPFWQRRSTTSSSSTQNPNQNYRSRHG--NRNTDIS---A 60

Query: 63  ASSSSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQT 122
            S    T+SSVA+SLLP RRRLRLDP++ L+FPYEPGKQV+SAI+LKNTS+SH AFKFQT
Sbjct: 61  VSKPPLTMSSVARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQT 120

Query: 123 TAPKSCYMRPPGGILAPGENFIATVFRFVEHPENNEK-PMDQKSKIKFKIMSLKVKGGTE 182
           TAPKSCYMRPPGG+LAPGE+  ATVF+FVEHPENNEK P++QKSK+KFKIMSLKVK G E
Sbjct: 121 TAPKSCYMRPPGGVLAPGESVFATVFKFVEHPENNEKQPLNQKSKVKFKIMSLKVKPGVE 180

Query: 183 YAPELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQ 242
           Y PELF+EQKDQV VE++LRV+F+D +RPSAALEKLKRQL EAEAA+EARKKPP ETG +
Sbjct: 181 YVPELFDEQKDQVAVEQVLRVIFIDADRPSAALEKLKRQLDEAEAAVEARKKPPPETGPR 240

Query: 243 VVGGEGLVIDEWKERREKYLARQQVGAVDSV 270
           VV GEGLVIDEWKERREKYLARQQV +VDS+
Sbjct: 241 VV-GEGLVIDEWKERREKYLARQQVESVDSL 265

BLAST of Lsi04G012760 vs. TAIR 10
Match: AT4G21450.1 (PapD-like superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.1e-85
Identity = 166/254 (65.35%), Postives = 201/254 (79.13%), Query Frame = 0

Query: 26  SGNTNSSSSSSTQNLHHSGH----------GPSDRNSTASNNMKASSSSKTVSSVAKSLL 85
           SG ++SS+SS   N H   H          G +  +   S   +  + S +VSSVAKS L
Sbjct: 43  SGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFL 102

Query: 86  PPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILA 145
           P +RRL+LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTTAPKSC+MRPPG ILA
Sbjct: 103 PTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILA 162

Query: 146 PGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPELFEEQKDQVTVER 205
           PGE  IATVF+FVE PENNEKPMDQ+S++KFKIMSLKVKG  +Y PELF+EQKD V+ E+
Sbjct: 163 PGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQ 222

Query: 206 ILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVGGEGLVIDEWKERRE 265
           ILRV+F+DPER + ALEKLKRQLAEA+AA+EARKKPP ETG +++ GEGLVIDEWKERRE
Sbjct: 223 ILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEETGPKMI-GEGLVIDEWKERRE 282

Query: 266 KYLARQQVGAVDSV 270
           +YLA+QQ    DSV
Sbjct: 283 RYLAQQQGEGADSV 295

BLAST of Lsi04G012760 vs. TAIR 10
Match: AT4G21450.3 (PapD-like superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 2.4e-81
Identity = 165/273 (60.44%), Postives = 201/273 (73.63%), Query Frame = 0

Query: 26  SGNTNSSSSSSTQNLHHSGH----------GPSDRNSTASNNMKASSSSKTVSSVAKSLL 85
           SG ++SS+SS   N H   H          G +  +   S   +  + S +VSSVAKS L
Sbjct: 43  SGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFL 102

Query: 86  PPRRRLRLDPNNSLFFPY-------------------EPGKQVKSAIRLKNTSRSHVAFK 145
           P +RRL+LDP+  L+FP+                   EPGKQV+SAI++KNTS+SHVAFK
Sbjct: 103 PTKRRLKLDPSEKLYFPWFVSEDLRSIDTSLKFLSFNEPGKQVRSAIKIKNTSKSHVAFK 162

Query: 146 FQTTAPKSCYMRPPGGILAPGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGG 205
           FQTTAPKSC+MRPPG ILAPGE  IATVF+FVE PENNEKPMDQ+S++KFKIMSLKVKG 
Sbjct: 163 FQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGP 222

Query: 206 TEYAPELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARKKPPVETG 265
            +Y PELF+EQKD V+ E+ILRV+F+DPER + ALEKLKRQLAEA+AA+EARKKPP ETG
Sbjct: 223 MDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEETG 282

Query: 266 HQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 270
            +++ GEGLVIDEWKERRE+YLA+QQ    DSV
Sbjct: 283 PKMI-GEGLVIDEWKERRERYLAQQQGEGADSV 314

BLAST of Lsi04G012760 vs. TAIR 10
Match: AT4G05060.1 (PapD-like superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 1.8e-76
Identity = 154/252 (61.11%), Postives = 195/252 (77.38%), Query Frame = 0

Query: 29  TNSSSSSST--------QNLHHSGHGPSDRNSTASNNMKASSSSKTVSSVAKSLLPPRRR 88
           T+SSS +S+        Q+ H   HGP        N+ +++++  ++SSVA+SLLP +RR
Sbjct: 40  TSSSSVTSSHLNQNYIHQSRHFQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRR 99

Query: 89  LRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPGENF 148
           L+LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTT PKSC+MRP G ILAPGE  
Sbjct: 100 LKLDPSAKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEI 159

Query: 149 IATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPELFEEQKDQVTVERILRVV 208
           IATVF+FVE PENNEKPM+QKS +KFKIMSLK+K  T+Y PELFEEQKD V+ E+++RVV
Sbjct: 160 IATVFKFVEPPENNEKPMEQKSGVKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVV 219

Query: 209 FVDPERPSAALEKLKRQLAEAEAALEARKKPPVETGHQVVG----GEGLVIDEWKERREK 268
           F+DPE P++ +EKLK QLAEA+AA EARKK        +VG    GEGLVIDEWK+RRE+
Sbjct: 220 FLDPENPNSMMEKLKSQLAEADAADEARKK----ASEGIVGPKPIGEGLVIDEWKQRRER 279

BLAST of Lsi04G012760 vs. TAIR 10
Match: AT4G21450.2 (PapD-like superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-50
Identity = 104/168 (61.90%), Postives = 126/168 (75.00%), Query Frame = 0

Query: 26  SGNTNSSSSSSTQNLHHSGH----------GPSDRNSTASNNMKASSSSKTVSSVAKSLL 85
           SG ++SS+SS   N H   H          G +  +   S   +  + S +VSSVAKS L
Sbjct: 43  SGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFL 102

Query: 86  PPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTTAPKSCYMRPPGGILA 145
           P +RRL+LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTTAPKSC+MRPPG ILA
Sbjct: 103 PTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILA 162

Query: 146 PGENFIATVFRFVEHPENNEKPMDQKSKIKFKIMSLKVKGGTEYAPEL 184
           PGE  IATVF+FVE PENNEKPMDQ+S++KFKIMSLKVKG  +Y PEL
Sbjct: 163 PGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPEL 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1ECE05.2e-9770.48Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=... [more]
Q8VYN23.0e-8465.35Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=... [more]
Q8LPQ72.5e-7561.11Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=... [more]
Q9SYC92.2e-0728.35Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=... [more]
B9DHD71.5e-0632.50Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A5A7T1W85.0e-13594.38Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A1S3BZ625.0e-13594.38vesicle-associated protein 4-1 OS=Cucumis melo OX=3656 GN=LOC103494990 PE=4 SV=1[more]
A0A5D3BAA21.1e-13494.01Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0LFW43.3e-13192.13MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G851700 PE=4 SV... [more]
A0A6J1INV34.8e-13090.71vesicle-associated protein 4-1-like OS=Cucurbita maxima OX=3661 GN=LOC111476917 ... [more]
Match NameE-valueIdentityDescription
XP_038900245.17.9e-14397.77vesicle-associated protein 4-1 [Benincasa hispida][more]
XP_008454625.11.0e-13494.38PREDICTED: vesicle-associated protein 4-1 [Cucumis melo] >KAA0036758.1 vesicle-a... [more]
TYJ95826.12.3e-13494.01vesicle-associated protein 4-1 [Cucumis melo var. makuwa][more]
XP_011652378.16.9e-13192.13vesicle-associated protein 4-1 [Cucumis sativus] >KGN59869.1 hypothetical protei... [more]
XP_022976559.11.0e-12990.71vesicle-associated protein 4-1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G54110.13.7e-9870.48membrane-associated mannitol-induced [more]
AT4G21450.12.1e-8565.35PapD-like superfamily protein [more]
AT4G21450.32.4e-8160.44PapD-like superfamily protein [more]
AT4G05060.11.8e-7661.11PapD-like superfamily protein [more]
AT4G21450.21.7e-5061.90PapD-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 208..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..71
NoneNo IPR availablePANTHERPTHR10809:SF110VESICLE-ASSOCIATED PROTEIN 4-1coord: 47..268
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 77..206
e-value: 7.9E-29
score: 102.1
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 81..191
e-value: 8.2E-29
score: 99.6
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 80..202
score: 22.209167
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 47..268
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 69..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G012760.1Lsi04G012760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0005886 plasma membrane