Lsi04G012400 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G012400 vs. ExPASy Swiss-Prot
Match: Q6NQ81 (Pentatricopeptide repeat-containing protein At4g04790, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g04790 PE=2 SV=2)

HSP 1 Score: 586.3 bits (1510), Expect = 6.7e-166
Identity = 342/901 (37.96%), Postives = 491/901 (54.50%), Query Frame = 0

Query: 114 NEKSLEK--LLDIPWFSIKSNHSMAVHRREISRERKQKWIFK-NSQNKRFSRLVKICAQK 173
           ++ SLEK   L IP F+ K  + +++  +E+SRERK++ ++K N  ++RF+++ +  AQK
Sbjct: 73  DKSSLEKNLFLKIPSFTTKIPYDISLRTKELSRERKERRVYKQNGLSRRFAKIFRDSAQK 132

Query: 174 LGTDLTLEVFGKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQG 233
           LGT+     F ++ +E  V EYNA+I + LE A+ S D++  L  + K ++L K M+++G
Sbjct: 133 LGTEAMFGAFDRVAKEMSVTEYNAMIGVYLEHAEKSNDLDYALGHIEKAFELLKSMRDRG 192

Query: 234 FQLEDETYGPVLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQ 293
           F +E+  YGP+L Y I MDMV+EF+ F + +++ +PGS  RLGYYEML ++ + D EK++
Sbjct: 193 FLIEERVYGPLLGYLIGMDMVDEFHSFKDVIREASPGSVERLGYYEMLLWIHLGDGEKIE 252

Query: 294 ELCYHATVDDGVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLG 353
           ELC     D+G     LQENYLLALC+ ++   L ++LE++DITK+ S+ +  NIF+ LG
Sbjct: 253 ELCSTIDGDNGESLSVLQENYLLALCKKDQKYHLERLLEIVDITKVRSSDLLANIFEYLG 312

Query: 354 RVSLHSFAKKLLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPS 413
           R SL S A + L   + S  G +N+  LI  Y+   PN  +ED + KF  MH ELD+ PS
Sbjct: 313 RFSLDSVASRFLWELRESDEGVKNVSDLISIYSTCTPNPTVEDTILKFNKMHEELDVMPS 372

Query: 414 SASYEKLICYCCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL------ 473
           S SYEKL+ Y C S +V  ALD+  ++ +A   +S D+LHS+LHA+DE  E++L      
Sbjct: 373 STSYEKLVKYSCDSNEVVTALDVVEKMGEAGLMISADILHSLLHAIDEVLEFDLVRRIHS 432

Query: 474 --------------------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRER 533
                                     F+ AY+ML   +  N+ P ++M+N I+AGYFRE 
Sbjct: 433 IMCTKSVKPNTENFRSIIRLCTRIKDFEGAYNMLGNLKNFNLEPNSSMFNCILAGYFRE- 492

Query: 534 VLGAGVGGLLLWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSK 593
                                                                       
Sbjct: 493 ------------------------------------------------------------ 552

Query: 594 MEYEPEMNMEVTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFE 653
                                                                       
Sbjct: 553 ------------------------------------------------------------ 612

Query: 654 KDDGEVLWMKSSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNC 713
                                       KN S  LMV+KQM+ AGVKPDS+TF YLINNC
Sbjct: 613 ----------------------------KNVSSALMVVKQMKEAGVKPDSITFGYLINNC 672

Query: 714 ECEEDIIKSPFFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKN 773
             E+ I K      YYEE+K +GVQ TK+I+M+LI+AYAA G+FEKAKQV+ D  +P  N
Sbjct: 673 TQEDAITK------YYEEMKQAGVQATKRIYMSLIDAYAASGKFEKAKQVLVDPDVPAIN 732

Query: 774 LNEVRSVLVSALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLL 833
            NE++SVL+SALAS G+ ADAL IY+EM++A C ++PK++ISL+E+    G L+ ++QL 
Sbjct: 733 QNELKSVLISALASRGKWADALHIYEEMRKAECHVDPKSIISLIEYSDSKGELSTLVQLA 792

Query: 834 GDLHHDLDDWIDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIA 893
            DL  D   WID   R+ILF++++   S  VDLLK+   R     + +   FDE+F  IA
Sbjct: 793 DDLQDD-TSWIDGFFRMILFAVRNKKSSDIVDLLKRNKVRLLKKGIPVEAHFDEVFWAIA 816

Query: 894 ESEPTYLETGLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPY 953
           E+EP+ +  G+ LL+F+K++LG  P R+CLDFLL AC NAKD E   L+WKEY++A  P 
Sbjct: 853 ETEPSKVHLGMDLLRFMKDELGFVPSRKCLDFLLHACVNAKDLEHGLLVWKEYQSAAFPC 816

Query: 954 NTISYLRMYQALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKRRKKM 980
           N +S+LRMYQ LLA+G  E AK L+ KIPKDD  V  II+E +  +  +P  KKK +KKM
Sbjct: 913 NVLSFLRMYQVLLAAGDSEGAKALVSKIPKDDKDVQHIIEESQSAFSQAPN-KKKPKKKM 816

BLAST of Lsi04G012400 vs. ExPASy Swiss-Prot
Match: O49711 (Pentatricopeptide repeat-containing protein At4g21880, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g21880 PE=3 SV=2)

HSP 1 Score: 532.7 bits (1371), Expect = 8.8e-150
Identity = 316/874 (36.16%), Postives = 459/874 (52.52%), Query Frame = 0

Query: 140 REISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEVFGKLGRETGVKEYNALIDMC 199
           ++ SR R     ++N Q++   +L K C +KLGT+   EV  K+G+E G KEYNA+  +C
Sbjct: 122 KQASRGRMLTENYQNKQSEIMEKLAKGCVRKLGTETMFEVLTKMGKEAGEKEYNAMTKLC 181

Query: 200 LEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYGPVLTYFIDMDMVEEFNFFCE 259
           ++ A+ S D E  L+Q+GK  +  K M++ GF + +  YGP   Y +DM+MV EF    +
Sbjct: 182 IQRARRSNDAEYALDQIGKAIEHLKEMRQLGFSIGEGAYGPFFKYLVDMEMVAEFQILKD 241

Query: 260 AVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVDDGVDKYSLQENYLLALCECE 319
            +K+  P S  RL YYEML ++++NDEEK+ +LC +   D G+    LQE YL+ALCE +
Sbjct: 242 FIKEACPESCGRLVYYEMLLWIQVNDEEKIHKLC-NKVDDSGLSLSILQEYYLVALCEKD 301

Query: 320 KTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAKKLLLAFK-TSGNGAENIPYL 379
             E   ++LE++DIT +SS    ++IF  LG+  L S A KLL   +    +G E +  L
Sbjct: 302 SKENFQKLLEIVDITTVSSPDALKSIFGYLGKSLLESVAMKLLWELRDCRKDGVETVSNL 361

Query: 380 IYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLICYCCGSLKVHMALDIANEIC 439
           I++YA  IPN  +ED + KF  +H ELDI PSS SYE L+ Y CGS +V  ALDI   +C
Sbjct: 362 IFSYATCIPNSTVEDAIFKFNKLHEELDIVPSSTSYENLVSYLCGSNEVVTALDIVENMC 421

Query: 440 DADFTLSTDVLHSILHALDEGCEYNL--------------------------------FK 499
           +A   +S ++LHS+L A+++  E+NL                                F+
Sbjct: 422 EAGLVISANILHSLLQAIEQILEFNLVQRIYSIMSNKSVKPNSETFRKSINLCIRIKDFE 481

Query: 500 AAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGLLLWLSQCLPKVPVLSSTSK 559
            AY+ML   +  N+ P ++MYN+IMAGYFRE                             
Sbjct: 482 GAYNMLGNLKNFNLAPNSSMYNSIMAGYFRE----------------------------- 541

Query: 560 QVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNMEVTLPLMVLQRHGLEIGLL 619
                                                                       
Sbjct: 542 ------------------------------------------------------------ 601

Query: 620 WRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWMKSSLLDRNPFSGTWIVGDV 679
                                                                       
Sbjct: 602 ------------------------------------------------------------ 661

Query: 680 KNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQYYEELKSSGVQVTK 739
           K  +  L VLK+M+ A VKPDS+TFSYLIN C  E  I K      YY+E+K +GV+V K
Sbjct: 662 KKVNSALKVLKEMKEADVKPDSVTFSYLINYCGEEATIAK------YYKEMKQAGVEVNK 721

Query: 740 QIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVSALASHGQTADALKIYDEM 799
            ++M+L+ AYA+ GQFEKAKQV+ D  +P K+ NE++SVL+SALAS+G   +AL IY+EM
Sbjct: 722 HVYMSLVKAYASCGQFEKAKQVLMDLEVPAKDHNELKSVLISALASNGNITEALSIYEEM 781

Query: 800 KQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDWIDCCHRIILFSIKHNDLS 859
           K+  C +EPKA++SL+E+   +  L  +++L  +L  D   WID   +II+F++++N  S
Sbjct: 782 KKLRCPVEPKAILSLIENSDSNAELGTLVELTHEL-RDSKFWIDGFFKIIVFAVRNNRSS 836

Query: 860 STVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLETGLQLLQFIKNDLGLSPPRR 919
           S +DLL+Q     S D+V +   F+E+F  IAE+E + ++ GL L+ F+K +L L P R+
Sbjct: 842 SILDLLEQTKNHLSKDDVGVEYWFEEVFKSIAETESSDVKVGLDLVSFMKEELELCPSRK 836

Query: 920 CLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQALLASGALESAKVLLDKI 979
           CLDFLL AC NAKD +S+ L+W+EY+ A LPYN I+YLRMYQ L+A+G  +SA+ ++ KI
Sbjct: 902 CLDFLLHACVNAKDKQSALLVWEEYQCAELPYNVINYLRMYQVLVAAGDSKSAEAIVSKI 836

Query: 980 PKDDAHVCWIIKECEMVYIASPTAKKKRRKKMLK 981
           P DD  V  IIKE  +V+  +P  KKK++K   K
Sbjct: 962 PNDDKDVKCIIKESRIVF--TPKLKKKKKKSKQK 836

BLAST of Lsi04G012400 vs. ExPASy Swiss-Prot
Match: O04659 (Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E14 PE=2 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 9.1e-06
Identity = 41/136 (30.15%), Postives = 71/136 (52.21%), Query Frame = 0

Query: 646 VKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQYYEELKSSGVQVT 705
           V N    + V  QM   GVKPD +TF+ ++  C     + K     Q +  +  S ++  
Sbjct: 388 VGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAALEKG---KQIHLSISESRLETD 447

Query: 706 KQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVSALASHGQTADALKIYDE 765
           + +  AL++ Y+  G  ++A ++ +   IP K++    +V++SA  SHGQ  +AL  +DE
Sbjct: 448 ELLLSALLDMYSKCGNEKEAFRIFN--SIPKKDVVS-WTVMISAYGSHGQPREALYQFDE 507

Query: 766 MKQAGCDLEPKAVISL 782
           M++ G  L+P  V  L
Sbjct: 508 MQKFG--LKPDGVTLL 515

BLAST of Lsi04G012400 vs. ExPASy Swiss-Prot
Match: Q8L844 (Pentatricopeptide repeat-containing protein At5g42310, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRP1 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.6e-05
Identity = 39/172 (22.67%), Postives = 83/172 (48.26%), Query Frame = 0

Query: 636 PFSGTWIVGDVKNTSD---GLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQ 695
           P +   ++G     +D    L ++ +M   G + D + +S +I +     + I S    +
Sbjct: 197 PLTYNALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSL-TRSNKIDSVMLLR 256

Query: 696 YYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVI---SDEGIPVKNLNEVRSVLVSA 755
            Y+E++   +++  Q+   +I  +A  G   KA Q++      G+  K    V   ++SA
Sbjct: 257 LYKEIERDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTATLVS--IISA 316

Query: 756 LASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDL 802
           LA  G+T +A  +++E++Q+G     +A  +L++ Y   GPL     ++ ++
Sbjct: 317 LADSGRTLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEM 365

BLAST of Lsi04G012400 vs. ExPASy Swiss-Prot
Match: Q9SXD8 (Pentatricopeptide repeat-containing protein At1g62590 OS=Arabidopsis thaliana OX=3702 GN=At1g62590 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.7e-05
Identity = 56/298 (18.79%), Postives = 127/298 (42.62%), Query Frame = 0

Query: 648 NTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQYYEELKSSGVQVTKQ 707
           +T   L +L +ME A ++ D + F+ +I++  C+   +        ++E+++ G++    
Sbjct: 240 DTDLALNLLNKMEAAKIEADVVIFNTIIDSL-CKYRHVDDAL--NLFKEMETKGIRPNVV 299

Query: 708 IFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVR-SVLVSALASHGQTADALKIYDEM 767
            + +LI+   ++G++  A Q++SD      N N V  + L+ A    G+  +A K+YD+M
Sbjct: 300 TYSSLISCLCSYGRWSDASQLLSDMIEKKINPNLVTFNALIDAFVKEGKFVEAEKLYDDM 359

Query: 768 KQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDWIDCCHRIILFS------I 827
            +   D +     SLV  +     L++  Q+   +        DC   ++ ++       
Sbjct: 360 IKRSIDPDIFTYNSLVNGFCMHDRLDKAKQMFEFMVSK-----DCFPDVVTYNTLIKGFC 419

Query: 828 KHNDLSSTVDLLKQLSYR-CSNDEVTMGVAFDEIFSLIAESEPTYLETGLQLLQFIKNDL 887
           K   +    +L +++S+R    D VT       +F           +   ++ + + +D 
Sbjct: 420 KSKRVEDGTELFREMSHRGLVGDTVTYTTLIQGLF------HDGDCDNAQKVFKQMVSD- 479

Query: 888 GLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQALLASGALE 938
           G+ P       LL    N    E +  ++   + + +  +   Y  M + +  +G ++
Sbjct: 480 GVPPDIMTYSILLDGLCNNGKLEKALEVFDYMQKSEIKLDIYIYTTMIEGMCKAGKVD 522

BLAST of Lsi04G012400 vs. ExPASy TrEMBL
Match: A0A6J1J1Y5 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480523 PE=4 SV=1)

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 693/1006 (68.89%), Postives = 749/1006 (74.45%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS AI KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAIDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILCGSFVRSPDAQGNGNEKSLEK 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILCGS VRSPDAQ NG+E SL++
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCGSSVRSPDAQENGDENSLKQ 120

Query: 121 LLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEVF 180
           LLDIPWFS  SNHS+++ RREISRERKQKWIFKNSQN RFS+LV+ CAQKLGTD+TLEVF
Sbjct: 121 LLDIPWFSNISNHSISLRRREISRERKQKWIFKNSQNYRFSQLVRNCAQKLGTDVTLEVF 180

Query: 181 GKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYGP 240
           GKLGRETGVKEYNALID+CLE+AK SKD+EVVLE + KVYQLFK+MKE GFQLEDETYGP
Sbjct: 181 GKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEHIAKVYQLFKLMKEHGFQLEDETYGP 240

Query: 241 VLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVDD 300
           VL Y IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HAT  D
Sbjct: 241 VLAYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHATAHD 300

Query: 301 GVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAKK 360
           GVDKYSLQENYLLALCECE+ EELLQMLE++DITKL+STV+A NIFKCLGR+ LHS A+K
Sbjct: 301 GVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLTSTVLAANIFKCLGRLLLHSIAEK 360

Query: 361 LLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLICY 420
           LL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMHFELDI PSS SYEKLICY
Sbjct: 361 LLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHFELDIKPSSVSYEKLICY 420

Query: 421 CCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL---------------- 480
           CCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL                
Sbjct: 421 CCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKPD 480

Query: 481 ----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGLL 540
                           F  AYDMLK SE+MN   TA+MYNAIMAGYFRE           
Sbjct: 481 SEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTSTASMYNAIMAGYFRE----------- 540

Query: 541 LWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNME 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 VTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWMK 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 SSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSP 720
                             KNTSDGLMVLKQMELA VKPDS TFSYLI+NCECEEDIIK  
Sbjct: 661 ------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISNCECEEDIIK-- 720

Query: 721 FFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVS 780
               YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLVS
Sbjct: 721 ----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLVS 780

Query: 781 ALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDW 840
           ALAS+ Q ADALKIYDEMKQAGC+LEPKAVISL+EHYPFDGP+NR+ QLLG+ HHDLD W
Sbjct: 781 ALASNAQIADALKIYDEMKQAGCNLEPKAVISLIEHYPFDGPMNRIFQLLGEFHHDLDHW 840

Query: 841 IDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLETG 900
           IDCC RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLETG
Sbjct: 841 IDCCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLETG 851

Query: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQ 960
           LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIWKEYE AGLP+NTISYLRMYQ
Sbjct: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWKEYEKAGLPHNTISYLRMYQ 851

Query: 961 ALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR 975
           ALLASG  +SAKVLL+KIPKDDAHVC++IKECE VY+AS + KKK+
Sbjct: 961 ALLASGDQKSAKVLLEKIPKDDAHVCYVIKECESVYVASSSVKKKK 851

BLAST of Lsi04G012400 vs. ExPASy TrEMBL
Match: A0A6J1FNL3 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445890 PE=4 SV=1)

HSP 1 Score: 1301.6 bits (3367), Expect = 0.0e+00
Identity = 698/1020 (68.43%), Postives = 756/1020 (74.12%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS A  KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAFDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILCGSFVRSPDAQGNGNEKSLEK 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILCGS VRSPDAQ NG+E SL++
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCGSSVRSPDAQENGDENSLKQ 120

Query: 121 LLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEVF 180
           LLDIPWFS  SNHS+++ RREISRERKQKWIFKNSQN RF++LV+ CAQKLGTD+TLEVF
Sbjct: 121 LLDIPWFSNMSNHSISLRRREISRERKQKWIFKNSQNYRFNQLVRNCAQKLGTDVTLEVF 180

Query: 181 GKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYGP 240
           GKLGRETGVKEYNALID+CLE+AK SKD+EVVLEQ+ KVYQLFK+MKE GFQLEDETYGP
Sbjct: 181 GKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEQIAKVYQLFKLMKEHGFQLEDETYGP 240

Query: 241 VLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVDD 300
           VLTY IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HA   D
Sbjct: 241 VLTYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHAIAHD 300

Query: 301 GVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAKK 360
           GVDKYSLQENYLLALCECE+ EELLQMLE++DITKLSSTV+A N+FKCLGR+ LHS A+K
Sbjct: 301 GVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLSSTVLAANVFKCLGRLLLHSIAEK 360

Query: 361 LLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLICY 420
           LL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMH ELDI PSS SYEKLICY
Sbjct: 361 LLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHLELDIKPSSVSYEKLICY 420

Query: 421 CCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL---------------- 480
           CCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL                
Sbjct: 421 CCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKPD 480

Query: 481 ----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGLL 540
                           F  AYDMLK SE+MN  PTA+MYNAIMAGYFRE           
Sbjct: 481 SEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTPTASMYNAIMAGYFRE----------- 540

Query: 541 LWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNME 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 VTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWMK 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 SSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSP 720
                             KNTSDGLMVLKQMELA VKPDS TFSYLI+ CECEEDIIK  
Sbjct: 661 ------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISYCECEEDIIK-- 720

Query: 721 FFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVS 780
               YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLVS
Sbjct: 721 ----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLVS 780

Query: 781 ALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDW 840
           ALAS+ Q ADALKIYDEMKQAGC+LEPKAVISLVEHYPFD P+NRM QLLG+LHHDLD W
Sbjct: 781 ALASNAQIADALKIYDEMKQAGCNLEPKAVISLVEHYPFDWPMNRMFQLLGELHHDLDVW 840

Query: 841 IDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLETG 900
           ID C RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLETG
Sbjct: 841 IDSCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLETG 865

Query: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQ 960
           LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIW+EYE AGLP+NTISYLRMYQ
Sbjct: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWEEYEKAGLPHNTISYLRMYQ 865

Query: 961 ALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR---RKKMLKMIRNK 986
           ALLASG  +SAKVLL+KIPKDDAHVC++IKECE VY+AS + KKK+   +KKMLKM  N+
Sbjct: 961 ALLASGDQKSAKVLLEKIPKDDAHVCYVIKECESVYVASFSVKKKKGRHKKKMLKMSINE 865

BLAST of Lsi04G012400 vs. ExPASy TrEMBL
Match: A0A6J1ITU1 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480523 PE=4 SV=1)

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 693/1007 (68.82%), Postives = 749/1007 (74.38%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS AI KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAIDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILC-GSFVRSPDAQGNGNEKSLE 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILC GS VRSPDAQ NG+E SL+
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCEGSSVRSPDAQENGDENSLK 120

Query: 121 KLLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEV 180
           +LLDIPWFS  SNHS+++ RREISRERKQKWIFKNSQN RFS+LV+ CAQKLGTD+TLEV
Sbjct: 121 QLLDIPWFSNISNHSISLRRREISRERKQKWIFKNSQNYRFSQLVRNCAQKLGTDVTLEV 180

Query: 181 FGKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYG 240
           FGKLGRETGVKEYNALID+CLE+AK SKD+EVVLE + KVYQLFK+MKE GFQLEDETYG
Sbjct: 181 FGKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEHIAKVYQLFKLMKEHGFQLEDETYG 240

Query: 241 PVLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVD 300
           PVL Y IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HAT  
Sbjct: 241 PVLAYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHATAH 300

Query: 301 DGVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAK 360
           DGVDKYSLQENYLLALCECE+ EELLQMLE++DITKL+STV+A NIFKCLGR+ LHS A+
Sbjct: 301 DGVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLTSTVLAANIFKCLGRLLLHSIAE 360

Query: 361 KLLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLIC 420
           KLL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMHFELDI PSS SYEKLIC
Sbjct: 361 KLLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHFELDIKPSSVSYEKLIC 420

Query: 421 YCCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL--------------- 480
           YCCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL               
Sbjct: 421 YCCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKP 480

Query: 481 -----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGL 540
                            F  AYDMLK SE+MN   TA+MYNAIMAGYFRE          
Sbjct: 481 DSEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTSTASMYNAIMAGYFRE---------- 540

Query: 541 LLWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNM 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 EVTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWM 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 KSSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKS 720
                              KNTSDGLMVLKQMELA VKPDS TFSYLI+NCECEEDIIK 
Sbjct: 661 -------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISNCECEEDIIK- 720

Query: 721 PFFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLV 780
                YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLV
Sbjct: 721 -----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLV 780

Query: 781 SALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDD 840
           SALAS+ Q ADALKIYDEMKQAGC+LEPKAVISL+EHYPFDGP+NR+ QLLG+ HHDLD 
Sbjct: 781 SALASNAQIADALKIYDEMKQAGCNLEPKAVISLIEHYPFDGPMNRIFQLLGEFHHDLDH 840

Query: 841 WIDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLET 900
           WIDCC RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLET
Sbjct: 841 WIDCCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLET 852

Query: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMY 960
           GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIWKEYE AGLP+NTISYLRMY
Sbjct: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWKEYEKAGLPHNTISYLRMY 852

Query: 961 QALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR 975
           QALLASG  +SAKVLL+KIPKDDAHVC++IKECE VY+AS + KKK+
Sbjct: 961 QALLASGDQKSAKVLLEKIPKDDAHVCYVIKECESVYVASSSVKKKK 852

BLAST of Lsi04G012400 vs. ExPASy TrEMBL
Match: A0A6J1FIW6 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445890 PE=4 SV=1)

HSP 1 Score: 1297.0 bits (3355), Expect = 0.0e+00
Identity = 698/1021 (68.36%), Postives = 756/1021 (74.05%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS A  KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAFDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILC-GSFVRSPDAQGNGNEKSLE 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILC GS VRSPDAQ NG+E SL+
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCEGSSVRSPDAQENGDENSLK 120

Query: 121 KLLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEV 180
           +LLDIPWFS  SNHS+++ RREISRERKQKWIFKNSQN RF++LV+ CAQKLGTD+TLEV
Sbjct: 121 QLLDIPWFSNMSNHSISLRRREISRERKQKWIFKNSQNYRFNQLVRNCAQKLGTDVTLEV 180

Query: 181 FGKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYG 240
           FGKLGRETGVKEYNALID+CLE+AK SKD+EVVLEQ+ KVYQLFK+MKE GFQLEDETYG
Sbjct: 181 FGKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEQIAKVYQLFKLMKEHGFQLEDETYG 240

Query: 241 PVLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVD 300
           PVLTY IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HA   
Sbjct: 241 PVLTYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHAIAH 300

Query: 301 DGVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAK 360
           DGVDKYSLQENYLLALCECE+ EELLQMLE++DITKLSSTV+A N+FKCLGR+ LHS A+
Sbjct: 301 DGVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLSSTVLAANVFKCLGRLLLHSIAE 360

Query: 361 KLLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLIC 420
           KLL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMH ELDI PSS SYEKLIC
Sbjct: 361 KLLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHLELDIKPSSVSYEKLIC 420

Query: 421 YCCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL--------------- 480
           YCCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL               
Sbjct: 421 YCCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKP 480

Query: 481 -----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGL 540
                            F  AYDMLK SE+MN  PTA+MYNAIMAGYFRE          
Sbjct: 481 DSEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTPTASMYNAIMAGYFRE---------- 540

Query: 541 LLWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNM 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 EVTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWM 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 KSSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKS 720
                              KNTSDGLMVLKQMELA VKPDS TFSYLI+ CECEEDIIK 
Sbjct: 661 -------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISYCECEEDIIK- 720

Query: 721 PFFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLV 780
                YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLV
Sbjct: 721 -----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLV 780

Query: 781 SALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDD 840
           SALAS+ Q ADALKIYDEMKQAGC+LEPKAVISLVEHYPFD P+NRM QLLG+LHHDLD 
Sbjct: 781 SALASNAQIADALKIYDEMKQAGCNLEPKAVISLVEHYPFDWPMNRMFQLLGELHHDLDV 840

Query: 841 WIDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLET 900
           WID C RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLET
Sbjct: 841 WIDSCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLET 866

Query: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMY 960
           GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIW+EYE AGLP+NTISYLRMY
Sbjct: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWEEYEKAGLPHNTISYLRMY 866

Query: 961 QALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR---RKKMLKMIRN 986
           QALLASG  +SAKVLL+KIPKDDAHVC++IKECE VY+AS + KKK+   +KKMLKM  N
Sbjct: 961 QALLASGDQKSAKVLLEKIPKDDAHVCYVIKECESVYVASFSVKKKKGRHKKKMLKMSIN 866

BLAST of Lsi04G012400 vs. ExPASy TrEMBL
Match: A0A6J1CCQ2 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111010449 PE=4 SV=1)

HSP 1 Score: 1247.3 bits (3226), Expect = 0.0e+00
Identity = 672/1021 (65.82%), Postives = 747/1021 (73.16%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           MRSKSK LSSLFR AI ASKPS+N +DA L++ VSTID FSPST LS AI  R IKS NS
Sbjct: 1   MRSKSKNLSSLFRSAIAASKPSQNLQDAALKDYVSTIDPFSPSTSLSKAIDYRYIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILC-GSFVRSPDAQGNGNEKSLE 120
           KK PQK NSDV+ P+L++EEPS SG+ MKHLT AISSILC GS V S DAQGNG++KS+E
Sbjct: 61  KKPPQKLNSDVQFPALVLEEPSGSGDSMKHLTKAISSILCEGSSVSSRDAQGNGDDKSVE 120

Query: 121 KLLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEV 180
           KLLDIPWFS  SNHS++  RREISRERKQKWIFK+SQ+ RF+RLV+ CAQKLGTD+TLEV
Sbjct: 121 KLLDIPWFSKISNHSISFRRREISRERKQKWIFKSSQSNRFNRLVRNCAQKLGTDVTLEV 180

Query: 181 FGKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYG 240
           FGKLGRETGVKEYNAL+D+CLE+AK SKDVE+VLEQ+ KVYQLFK+MKEQGFQ+EDETYG
Sbjct: 181 FGKLGRETGVKEYNALMDICLEKAKTSKDVEIVLEQIAKVYQLFKLMKEQGFQIEDETYG 240

Query: 241 PVLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVD 300
           PVLTY I+MDM+EEF+FFCEA+KDG+PGS SRLGYYEMLFYVKIND EKVQELC HA  +
Sbjct: 241 PVLTYLIEMDMMEEFDFFCEAIKDGHPGSYSRLGYYEMLFYVKINDGEKVQELCKHAATN 300

Query: 301 DGVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAK 360
           DGVDKY+LQENYLLALCECE+  E+LQMLE+IDITKLSSTV+A NIF+CL R+SLHSFA+
Sbjct: 301 DGVDKYNLQENYLLALCECEQKNEILQMLEIIDITKLSSTVLAVNIFECLARLSLHSFAE 360

Query: 361 KLLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLIC 420
           KLL+AFKTSG GA NIPYLIY+YAVSIPNLAIEDMVSKFKS+H +LDI PSS SYEKLI 
Sbjct: 361 KLLVAFKTSGTGAGNIPYLIYSYAVSIPNLAIEDMVSKFKSLHSKLDIKPSSTSYEKLIH 420

Query: 421 YCCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL--------------- 480
           YCCGS KVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL               
Sbjct: 421 YCCGSFKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKP 480

Query: 481 -----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGL 540
                            F  A+DMLK SE+MN+ PT++MYNAIMAGYFRE          
Sbjct: 481 DCETFRSIISLRVKMKDFNGAFDMLKESEKMNLTPTSSMYNAIMAGYFRE---------- 540

Query: 541 LLWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNM 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 EVTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWM 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 KSSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKS 720
                              KNTS  LMVLKQMELA VKPDS TFSYLI+NCECEEDIIK 
Sbjct: 661 -------------------KNTSGALMVLKQMELADVKPDSKTFSYLISNCECEEDIIK- 720

Query: 721 PFFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLV 780
                YYEELKSSG+Q TKQIFMALINAYAAHGQFEKAKQVI DEGIP+KNLNE+RSVLV
Sbjct: 721 -----YYEELKSSGIQATKQIFMALINAYAAHGQFEKAKQVILDEGIPMKNLNEIRSVLV 780

Query: 781 SALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDD 840
           SALAS+GQT+DALK+YDEMK+AGCDLEPKA ISL+EHYP DGPL+RMLQLL +L + LDD
Sbjct: 781 SALASNGQTSDALKVYDEMKEAGCDLEPKAAISLIEHYPLDGPLSRMLQLLAEL-NGLDD 840

Query: 841 WIDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLET 900
           WIDCC RIILFS+KHNDLSST+DLLKQLSYRC NDEV M VAFDE+FS I ES+PTYLET
Sbjct: 841 WIDCCRRIILFSLKHNDLSSTLDLLKQLSYRCCNDEVIMRVAFDEVFSFITESDPTYLET 865

Query: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMY 960
           GL LLQFIK DLGLSPPRRCLDFLLGACANAKDAESSRL+WKEY+ AGLPYNTIS+LRMY
Sbjct: 901 GLLLLQFIKKDLGLSPPRRCLDFLLGACANAKDAESSRLVWKEYKKAGLPYNTISFLRMY 865

Query: 961 QALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKK---KRRKKMLKMIRN 986
           QALLASG   SAKVLLDKIPKDD HVC IIKECEMVY AS + KK   + +KKMLKM R 
Sbjct: 961 QALLASGDQTSAKVLLDKIPKDDTHVCSIIKECEMVYGASSSVKKTKGRNKKKMLKMSRT 865

BLAST of Lsi04G012400 vs. NCBI nr
Match: XP_023524929.1 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1312.7 bits (3396), Expect = 0.0e+00
Identity = 701/1019 (68.79%), Postives = 759/1019 (74.48%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS AI KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAIDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILCGSFVRSPDAQGNGNEKSLEK 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILCGS VRSP+AQ NG+E SL++
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCGSSVRSPNAQENGDENSLKQ 120

Query: 121 LLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEVF 180
           LLDIPWFS  SN+S+++ RREISRERKQKWIFKNSQN RFS+LV+ CAQKLGTD+TLEVF
Sbjct: 121 LLDIPWFSNMSNYSISLRRREISRERKQKWIFKNSQNYRFSQLVRNCAQKLGTDVTLEVF 180

Query: 181 GKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYGP 240
           GKLGRETGVKEYNALID+CLE+AK SKD+EVVLEQ+ KVYQLFK+MKE GFQLEDETYGP
Sbjct: 181 GKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEQIAKVYQLFKLMKEHGFQLEDETYGP 240

Query: 241 VLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVDD 300
           VLTY IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HA   D
Sbjct: 241 VLTYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHAIAHD 300

Query: 301 GVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAKK 360
           GVDKYSLQENYLLALCECE+ EELLQMLE++DITKLSSTV+A NIFKCLGR+ LHS A+K
Sbjct: 301 GVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLSSTVLAANIFKCLGRLLLHSIAEK 360

Query: 361 LLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLICY 420
           LL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMHF+LDI PSS SYEKLICY
Sbjct: 361 LLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHFDLDIKPSSVSYEKLICY 420

Query: 421 CCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL---------------- 480
           CCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL                
Sbjct: 421 CCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKPD 480

Query: 481 ----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGLL 540
                           F  AYDMLK SE+MN  PTA+MYNAIMAGYFRE           
Sbjct: 481 SEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTPTASMYNAIMAGYFRE----------- 540

Query: 541 LWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNME 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 VTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWMK 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 SSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSP 720
                             KNTSDGLMVLKQMELA VKPDS TFSYLI+ CECEEDIIK  
Sbjct: 661 ------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISYCECEEDIIK-- 720

Query: 721 FFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVS 780
               YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLVS
Sbjct: 721 ----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLVS 780

Query: 781 ALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDW 840
           ALAS+ Q ADALKIYDEMKQAGC+LEPKAVISL+EHYPFDGP+NR+ QLLG+LHHDLDDW
Sbjct: 781 ALASNAQLADALKIYDEMKQAGCNLEPKAVISLIEHYPFDGPVNRIFQLLGELHHDLDDW 840

Query: 841 IDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLETG 900
           IDCC RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLETG
Sbjct: 841 IDCCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLETG 864

Query: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQ 960
           LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIWKEYE AGLP+NTISYLRMYQ
Sbjct: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWKEYEKAGLPHNTISYLRMYQ 864

Query: 961 ALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR---RKKMLKMIRN 985
           ALLASG   SAKVLL+KIPKDDAHVC++IKECE VY+AS +  KK+   +KKMLKM RN
Sbjct: 961 ALLASGDQTSAKVLLEKIPKDDAHVCYVIKECESVYVASSSVNKKKGRHKKKMLKMSRN 864

BLAST of Lsi04G012400 vs. NCBI nr
Match: XP_023524928.1 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1308.1 bits (3384), Expect = 0.0e+00
Identity = 701/1020 (68.73%), Postives = 759/1020 (74.41%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS AI KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAIDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILC-GSFVRSPDAQGNGNEKSLE 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILC GS VRSP+AQ NG+E SL+
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCEGSSVRSPNAQENGDENSLK 120

Query: 121 KLLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEV 180
           +LLDIPWFS  SN+S+++ RREISRERKQKWIFKNSQN RFS+LV+ CAQKLGTD+TLEV
Sbjct: 121 QLLDIPWFSNMSNYSISLRRREISRERKQKWIFKNSQNYRFSQLVRNCAQKLGTDVTLEV 180

Query: 181 FGKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYG 240
           FGKLGRETGVKEYNALID+CLE+AK SKD+EVVLEQ+ KVYQLFK+MKE GFQLEDETYG
Sbjct: 181 FGKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEQIAKVYQLFKLMKEHGFQLEDETYG 240

Query: 241 PVLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVD 300
           PVLTY IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HA   
Sbjct: 241 PVLTYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHAIAH 300

Query: 301 DGVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAK 360
           DGVDKYSLQENYLLALCECE+ EELLQMLE++DITKLSSTV+A NIFKCLGR+ LHS A+
Sbjct: 301 DGVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLSSTVLAANIFKCLGRLLLHSIAE 360

Query: 361 KLLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLIC 420
           KLL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMHF+LDI PSS SYEKLIC
Sbjct: 361 KLLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHFDLDIKPSSVSYEKLIC 420

Query: 421 YCCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL--------------- 480
           YCCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL               
Sbjct: 421 YCCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKP 480

Query: 481 -----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGL 540
                            F  AYDMLK SE+MN  PTA+MYNAIMAGYFRE          
Sbjct: 481 DSEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTPTASMYNAIMAGYFRE---------- 540

Query: 541 LLWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNM 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 EVTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWM 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 KSSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKS 720
                              KNTSDGLMVLKQMELA VKPDS TFSYLI+ CECEEDIIK 
Sbjct: 661 -------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISYCECEEDIIK- 720

Query: 721 PFFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLV 780
                YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLV
Sbjct: 721 -----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLV 780

Query: 781 SALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDD 840
           SALAS+ Q ADALKIYDEMKQAGC+LEPKAVISL+EHYPFDGP+NR+ QLLG+LHHDLDD
Sbjct: 781 SALASNAQLADALKIYDEMKQAGCNLEPKAVISLIEHYPFDGPVNRIFQLLGELHHDLDD 840

Query: 841 WIDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLET 900
           WIDCC RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLET
Sbjct: 841 WIDCCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLET 865

Query: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMY 960
           GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIWKEYE AGLP+NTISYLRMY
Sbjct: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWKEYEKAGLPHNTISYLRMY 865

Query: 961 QALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR---RKKMLKMIRN 985
           QALLASG   SAKVLL+KIPKDDAHVC++IKECE VY+AS +  KK+   +KKMLKM RN
Sbjct: 961 QALLASGDQTSAKVLLEKIPKDDAHVCYVIKECESVYVASSSVNKKKGRHKKKMLKMSRN 865

BLAST of Lsi04G012400 vs. NCBI nr
Match: XP_022981388.1 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 693/1006 (68.89%), Postives = 749/1006 (74.45%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS AI KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAIDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILCGSFVRSPDAQGNGNEKSLEK 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILCGS VRSPDAQ NG+E SL++
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCGSSVRSPDAQENGDENSLKQ 120

Query: 121 LLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEVF 180
           LLDIPWFS  SNHS+++ RREISRERKQKWIFKNSQN RFS+LV+ CAQKLGTD+TLEVF
Sbjct: 121 LLDIPWFSNISNHSISLRRREISRERKQKWIFKNSQNYRFSQLVRNCAQKLGTDVTLEVF 180

Query: 181 GKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYGP 240
           GKLGRETGVKEYNALID+CLE+AK SKD+EVVLE + KVYQLFK+MKE GFQLEDETYGP
Sbjct: 181 GKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEHIAKVYQLFKLMKEHGFQLEDETYGP 240

Query: 241 VLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVDD 300
           VL Y IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HAT  D
Sbjct: 241 VLAYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHATAHD 300

Query: 301 GVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAKK 360
           GVDKYSLQENYLLALCECE+ EELLQMLE++DITKL+STV+A NIFKCLGR+ LHS A+K
Sbjct: 301 GVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLTSTVLAANIFKCLGRLLLHSIAEK 360

Query: 361 LLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLICY 420
           LL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMHFELDI PSS SYEKLICY
Sbjct: 361 LLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHFELDIKPSSVSYEKLICY 420

Query: 421 CCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL---------------- 480
           CCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL                
Sbjct: 421 CCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKPD 480

Query: 481 ----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGLL 540
                           F  AYDMLK SE+MN   TA+MYNAIMAGYFRE           
Sbjct: 481 SEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTSTASMYNAIMAGYFRE----------- 540

Query: 541 LWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNME 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 VTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWMK 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 SSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSP 720
                             KNTSDGLMVLKQMELA VKPDS TFSYLI+NCECEEDIIK  
Sbjct: 661 ------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISNCECEEDIIK-- 720

Query: 721 FFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVS 780
               YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLVS
Sbjct: 721 ----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLVS 780

Query: 781 ALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDW 840
           ALAS+ Q ADALKIYDEMKQAGC+LEPKAVISL+EHYPFDGP+NR+ QLLG+ HHDLD W
Sbjct: 781 ALASNAQIADALKIYDEMKQAGCNLEPKAVISLIEHYPFDGPMNRIFQLLGEFHHDLDHW 840

Query: 841 IDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLETG 900
           IDCC RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLETG
Sbjct: 841 IDCCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLETG 851

Query: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQ 960
           LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIWKEYE AGLP+NTISYLRMYQ
Sbjct: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWKEYEKAGLPHNTISYLRMYQ 851

Query: 961 ALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR 975
           ALLASG  +SAKVLL+KIPKDDAHVC++IKECE VY+AS + KKK+
Sbjct: 961 ALLASGDQKSAKVLLEKIPKDDAHVCYVIKECESVYVASSSVKKKK 851

BLAST of Lsi04G012400 vs. NCBI nr
Match: XP_022940188.1 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1301.6 bits (3367), Expect = 0.0e+00
Identity = 698/1020 (68.43%), Postives = 756/1020 (74.12%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS A  KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAFDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILCGSFVRSPDAQGNGNEKSLEK 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILCGS VRSPDAQ NG+E SL++
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCGSSVRSPDAQENGDENSLKQ 120

Query: 121 LLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEVF 180
           LLDIPWFS  SNHS+++ RREISRERKQKWIFKNSQN RF++LV+ CAQKLGTD+TLEVF
Sbjct: 121 LLDIPWFSNMSNHSISLRRREISRERKQKWIFKNSQNYRFNQLVRNCAQKLGTDVTLEVF 180

Query: 181 GKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYGP 240
           GKLGRETGVKEYNALID+CLE+AK SKD+EVVLEQ+ KVYQLFK+MKE GFQLEDETYGP
Sbjct: 181 GKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEQIAKVYQLFKLMKEHGFQLEDETYGP 240

Query: 241 VLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVDD 300
           VLTY IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HA   D
Sbjct: 241 VLTYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHAIAHD 300

Query: 301 GVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAKK 360
           GVDKYSLQENYLLALCECE+ EELLQMLE++DITKLSSTV+A N+FKCLGR+ LHS A+K
Sbjct: 301 GVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLSSTVLAANVFKCLGRLLLHSIAEK 360

Query: 361 LLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLICY 420
           LL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMH ELDI PSS SYEKLICY
Sbjct: 361 LLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHLELDIKPSSVSYEKLICY 420

Query: 421 CCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL---------------- 480
           CCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL                
Sbjct: 421 CCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKPD 480

Query: 481 ----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGLL 540
                           F  AYDMLK SE+MN  PTA+MYNAIMAGYFRE           
Sbjct: 481 SEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTPTASMYNAIMAGYFRE----------- 540

Query: 541 LWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNME 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 VTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWMK 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 SSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSP 720
                             KNTSDGLMVLKQMELA VKPDS TFSYLI+ CECEEDIIK  
Sbjct: 661 ------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISYCECEEDIIK-- 720

Query: 721 FFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVS 780
               YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLVS
Sbjct: 721 ----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLVS 780

Query: 781 ALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDW 840
           ALAS+ Q ADALKIYDEMKQAGC+LEPKAVISLVEHYPFD P+NRM QLLG+LHHDLD W
Sbjct: 781 ALASNAQIADALKIYDEMKQAGCNLEPKAVISLVEHYPFDWPMNRMFQLLGELHHDLDVW 840

Query: 841 IDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLETG 900
           ID C RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLETG
Sbjct: 841 IDSCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLETG 865

Query: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQ 960
           LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIW+EYE AGLP+NTISYLRMYQ
Sbjct: 901 LQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWEEYEKAGLPHNTISYLRMYQ 865

Query: 961 ALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR---RKKMLKMIRNK 986
           ALLASG  +SAKVLL+KIPKDDAHVC++IKECE VY+AS + KKK+   +KKMLKM  N+
Sbjct: 961 ALLASGDQKSAKVLLEKIPKDDAHVCYVIKECESVYVASFSVKKKKGRHKKKMLKMSINE 865

BLAST of Lsi04G012400 vs. NCBI nr
Match: XP_022981387.1 (pentatricopeptide repeat-containing protein At4g04790, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 693/1007 (68.82%), Postives = 749/1007 (74.38%), Query Frame = 0

Query: 1   MRSKSKQLSSLFRFAIIASKPSKNPRDADLQNDVSTIDHFSPSTFLSNAIHKRSIKSPNS 60
           M SKSK LSSLFR AIIASKPS+NP+DA L+N VS ID FSPST LS AI KRSIKS NS
Sbjct: 1   MPSKSKNLSSLFRSAIIASKPSQNPQDAALKNYVSAIDPFSPSTSLSKAIDKRSIKSANS 60

Query: 61  KKLPQKPNSDVKLPSLIIEEPSESGEYMKHLTNAISSILC-GSFVRSPDAQGNGNEKSLE 120
           KKLPQK NSDV+ P+LI+EEPS SG+ MKHLT AISSILC GS VRSPDAQ NG+E SL+
Sbjct: 61  KKLPQKLNSDVQFPALILEEPSGSGDSMKHLTKAISSILCEGSSVRSPDAQENGDENSLK 120

Query: 121 KLLDIPWFSIKSNHSMAVHRREISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEV 180
           +LLDIPWFS  SNHS+++ RREISRERKQKWIFKNSQN RFS+LV+ CAQKLGTD+TLEV
Sbjct: 121 QLLDIPWFSNISNHSISLRRREISRERKQKWIFKNSQNYRFSQLVRNCAQKLGTDVTLEV 180

Query: 181 FGKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYG 240
           FGKLGRETGVKEYNALID+CLE+AK SKD+EVVLE + KVYQLFK+MKE GFQLEDETYG
Sbjct: 181 FGKLGRETGVKEYNALIDICLEKAKTSKDLEVVLEHIAKVYQLFKLMKEHGFQLEDETYG 240

Query: 241 PVLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVD 300
           PVL Y IDMDM+EEFNFFCEAVKDGNPGS SRLGYYEMLFYVKIND EK+QELC HAT  
Sbjct: 241 PVLAYLIDMDMMEEFNFFCEAVKDGNPGSNSRLGYYEMLFYVKINDVEKIQELCEHATAH 300

Query: 301 DGVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAK 360
           DGVDKYSLQENYLLALCECE+ EELLQMLE++DITKL+STV+A NIFKCLGR+ LHS A+
Sbjct: 301 DGVDKYSLQENYLLALCECEQKEELLQMLEIVDITKLTSTVLAANIFKCLGRLLLHSIAE 360

Query: 361 KLLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLIC 420
           KLL+A KTSGNGAENIPYLIYN+ VSIPNLAIEDMVSKFKSMHFELDI PSS SYEKLIC
Sbjct: 361 KLLVALKTSGNGAENIPYLIYNHVVSIPNLAIEDMVSKFKSMHFELDIKPSSVSYEKLIC 420

Query: 421 YCCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL--------------- 480
           YCCGSLKVHMALDIANE+CDADFT STDVLHSILHA+DEGCE+NL               
Sbjct: 421 YCCGSLKVHMALDIANEMCDADFTPSTDVLHSILHAMDEGCEFNLVHQVYSLICRHNLKP 480

Query: 481 -----------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGL 540
                            F  AYDMLK SE+MN   TA+MYNAIMAGYFRE          
Sbjct: 481 DSEVFRRMISLCIKMKDFNGAYDMLKESEKMNFTSTASMYNAIMAGYFRE---------- 540

Query: 541 LLWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNM 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 EVTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWM 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 KSSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKS 720
                              KNTSDGLMVLKQMELA VKPDS TFSYLI+NCECEEDIIK 
Sbjct: 661 -------------------KNTSDGLMVLKQMELADVKPDSKTFSYLISNCECEEDIIK- 720

Query: 721 PFFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLV 780
                YYEELKSSGVQ TK IFMALINAYAAHGQFEKAKQVISDE IPVKNLNE+RSVLV
Sbjct: 721 -----YYEELKSSGVQATKHIFMALINAYAAHGQFEKAKQVISDEEIPVKNLNEIRSVLV 780

Query: 781 SALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDD 840
           SALAS+ Q ADALKIYDEMKQAGC+LEPKAVISL+EHYPFDGP+NR+ QLLG+ HHDLD 
Sbjct: 781 SALASNAQIADALKIYDEMKQAGCNLEPKAVISLIEHYPFDGPMNRIFQLLGEFHHDLDH 840

Query: 841 WIDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLET 900
           WIDCC RI+LFS+KHNDLSSTVDLLKQLSYRC NDEV MGVAFDEIFSLIAESEP+YLET
Sbjct: 841 WIDCCRRILLFSVKHNDLSSTVDLLKQLSYRCCNDEVMMGVAFDEIFSLIAESEPSYLET 852

Query: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMY 960
           GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESS LIWKEYE AGLP+NTISYLRMY
Sbjct: 901 GLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSLLIWKEYEKAGLPHNTISYLRMY 852

Query: 961 QALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKR 975
           QALLASG  +SAKVLL+KIPKDDAHVC++IKECE VY+AS + KKK+
Sbjct: 961 QALLASGDQKSAKVLLEKIPKDDAHVCYVIKECESVYVASSSVKKKK 852

BLAST of Lsi04G012400 vs. TAIR 10
Match: AT4G04790.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 586.3 bits (1510), Expect = 4.7e-167
Identity = 342/901 (37.96%), Postives = 491/901 (54.50%), Query Frame = 0

Query: 114 NEKSLEK--LLDIPWFSIKSNHSMAVHRREISRERKQKWIFK-NSQNKRFSRLVKICAQK 173
           ++ SLEK   L IP F+ K  + +++  +E+SRERK++ ++K N  ++RF+++ +  AQK
Sbjct: 73  DKSSLEKNLFLKIPSFTTKIPYDISLRTKELSRERKERRVYKQNGLSRRFAKIFRDSAQK 132

Query: 174 LGTDLTLEVFGKLGRETGVKEYNALIDMCLEEAKASKDVEVVLEQVGKVYQLFKVMKEQG 233
           LGT+     F ++ +E  V EYNA+I + LE A+ S D++  L  + K ++L K M+++G
Sbjct: 133 LGTEAMFGAFDRVAKEMSVTEYNAMIGVYLEHAEKSNDLDYALGHIEKAFELLKSMRDRG 192

Query: 234 FQLEDETYGPVLTYFIDMDMVEEFNFFCEAVKDGNPGSKSRLGYYEMLFYVKINDEEKVQ 293
           F +E+  YGP+L Y I MDMV+EF+ F + +++ +PGS  RLGYYEML ++ + D EK++
Sbjct: 193 FLIEERVYGPLLGYLIGMDMVDEFHSFKDVIREASPGSVERLGYYEMLLWIHLGDGEKIE 252

Query: 294 ELCYHATVDDGVDKYSLQENYLLALCECEKTEELLQMLEVIDITKLSSTVVAENIFKCLG 353
           ELC     D+G     LQENYLLALC+ ++   L ++LE++DITK+ S+ +  NIF+ LG
Sbjct: 253 ELCSTIDGDNGESLSVLQENYLLALCKKDQKYHLERLLEIVDITKVRSSDLLANIFEYLG 312

Query: 354 RVSLHSFAKKLLLAFKTSGNGAENIPYLIYNYAVSIPNLAIEDMVSKFKSMHFELDINPS 413
           R SL S A + L   + S  G +N+  LI  Y+   PN  +ED + KF  MH ELD+ PS
Sbjct: 313 RFSLDSVASRFLWELRESDEGVKNVSDLISIYSTCTPNPTVEDTILKFNKMHEELDVMPS 372

Query: 414 SASYEKLICYCCGSLKVHMALDIANEICDADFTLSTDVLHSILHALDEGCEYNL------ 473
           S SYEKL+ Y C S +V  ALD+  ++ +A   +S D+LHS+LHA+DE  E++L      
Sbjct: 373 STSYEKLVKYSCDSNEVVTALDVVEKMGEAGLMISADILHSLLHAIDEVLEFDLVRRIHS 432

Query: 474 --------------------------FKAAYDMLKVSEEMNIIPTANMYNAIMAGYFRER 533
                                     F+ AY+ML   +  N+ P ++M+N I+AGYFRE 
Sbjct: 433 IMCTKSVKPNTENFRSIIRLCTRIKDFEGAYNMLGNLKNFNLEPNSSMFNCILAGYFRE- 492

Query: 534 VLGAGVGGLLLWLSQCLPKVPVLSSTSKQVNQKVEDEKQKTFVDVVVGSRESELHGSVSK 593
                                                                       
Sbjct: 493 ------------------------------------------------------------ 552

Query: 594 MEYEPEMNMEVTLPLMVLQRHGLEIGLLWRMDHGLVHPLSLENQRYCLFGGWGRGLVEFE 653
                                                                       
Sbjct: 553 ------------------------------------------------------------ 612

Query: 654 KDDGEVLWMKSSLLDRNPFSGTWIVGDVKNTSDGLMVLKQMELAGVKPDSMTFSYLINNC 713
                                       KN S  LMV+KQM+ AGVKPDS+TF YLINNC
Sbjct: 613 ----------------------------KNVSSALMVVKQMKEAGVKPDSITFGYLINNC 672

Query: 714 ECEEDIIKSPFFPQYYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVISDEGIPVKN 773
             E+ I K      YYEE+K +GVQ TK+I+M+LI+AYAA G+FEKAKQV+ D  +P  N
Sbjct: 673 TQEDAITK------YYEEMKQAGVQATKRIYMSLIDAYAASGKFEKAKQVLVDPDVPAIN 732

Query: 774 LNEVRSVLVSALASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLL 833
            NE++SVL+SALAS G+ ADAL IY+EM++A C ++PK++ISL+E+    G L+ ++QL 
Sbjct: 733 QNELKSVLISALASRGKWADALHIYEEMRKAECHVDPKSIISLIEYSDSKGELSTLVQLA 792

Query: 834 GDLHHDLDDWIDCCHRIILFSIKHNDLSSTVDLLKQLSYRCSNDEVTMGVAFDEIFSLIA 893
            DL  D   WID   R+ILF++++   S  VDLLK+   R     + +   FDE+F  IA
Sbjct: 793 DDLQDD-TSWIDGFFRMILFAVRNKKSSDIVDLLKRNKVRLLKKGIPVEAHFDEVFWAIA 816

Query: 894 ESEPTYLETGLQLLQFIKNDLGLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPY 953
           E+EP+ +  G+ LL+F+K++LG  P R+CLDFLL AC NAKD E   L+WKEY++A  P 
Sbjct: 853 ETEPSKVHLGMDLLRFMKDELGFVPSRKCLDFLLHACVNAKDLEHGLLVWKEYQSAAFPC 816

Query: 954 NTISYLRMYQALLASGALESAKVLLDKIPKDDAHVCWIIKECEMVYIASPTAKKKRRKKM 980
           N +S+LRMYQ LLA+G  E AK L+ KIPKDD  V  II+E +  +  +P  KKK +KKM
Sbjct: 913 NVLSFLRMYQVLLAAGDSEGAKALVSKIPKDDKDVQHIIEESQSAFSQAPN-KKKPKKKM 816

BLAST of Lsi04G012400 vs. TAIR 10
Match: AT4G21880.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 532.7 bits (1371), Expect = 6.2e-151
Identity = 316/874 (36.16%), Postives = 459/874 (52.52%), Query Frame = 0

Query: 140 REISRERKQKWIFKNSQNKRFSRLVKICAQKLGTDLTLEVFGKLGRETGVKEYNALIDMC 199
           ++ SR R     ++N Q++   +L K C +KLGT+   EV  K+G+E G KEYNA+  +C
Sbjct: 122 KQASRGRMLTENYQNKQSEIMEKLAKGCVRKLGTETMFEVLTKMGKEAGEKEYNAMTKLC 181

Query: 200 LEEAKASKDVEVVLEQVGKVYQLFKVMKEQGFQLEDETYGPVLTYFIDMDMVEEFNFFCE 259
           ++ A+ S D E  L+Q+GK  +  K M++ GF + +  YGP   Y +DM+MV EF    +
Sbjct: 182 IQRARRSNDAEYALDQIGKAIEHLKEMRQLGFSIGEGAYGPFFKYLVDMEMVAEFQILKD 241

Query: 260 AVKDGNPGSKSRLGYYEMLFYVKINDEEKVQELCYHATVDDGVDKYSLQENYLLALCECE 319
            +K+  P S  RL YYEML ++++NDEEK+ +LC +   D G+    LQE YL+ALCE +
Sbjct: 242 FIKEACPESCGRLVYYEMLLWIQVNDEEKIHKLC-NKVDDSGLSLSILQEYYLVALCEKD 301

Query: 320 KTEELLQMLEVIDITKLSSTVVAENIFKCLGRVSLHSFAKKLLLAFK-TSGNGAENIPYL 379
             E   ++LE++DIT +SS    ++IF  LG+  L S A KLL   +    +G E +  L
Sbjct: 302 SKENFQKLLEIVDITTVSSPDALKSIFGYLGKSLLESVAMKLLWELRDCRKDGVETVSNL 361

Query: 380 IYNYAVSIPNLAIEDMVSKFKSMHFELDINPSSASYEKLICYCCGSLKVHMALDIANEIC 439
           I++YA  IPN  +ED + KF  +H ELDI PSS SYE L+ Y CGS +V  ALDI   +C
Sbjct: 362 IFSYATCIPNSTVEDAIFKFNKLHEELDIVPSSTSYENLVSYLCGSNEVVTALDIVENMC 421

Query: 440 DADFTLSTDVLHSILHALDEGCEYNL--------------------------------FK 499
           +A   +S ++LHS+L A+++  E+NL                                F+
Sbjct: 422 EAGLVISANILHSLLQAIEQILEFNLVQRIYSIMSNKSVKPNSETFRKSINLCIRIKDFE 481

Query: 500 AAYDMLKVSEEMNIIPTANMYNAIMAGYFRERVLGAGVGGLLLWLSQCLPKVPVLSSTSK 559
            AY+ML   +  N+ P ++MYN+IMAGYFRE                             
Sbjct: 482 GAYNMLGNLKNFNLAPNSSMYNSIMAGYFRE----------------------------- 541

Query: 560 QVNQKVEDEKQKTFVDVVVGSRESELHGSVSKMEYEPEMNMEVTLPLMVLQRHGLEIGLL 619
                                                                       
Sbjct: 542 ------------------------------------------------------------ 601

Query: 620 WRMDHGLVHPLSLENQRYCLFGGWGRGLVEFEKDDGEVLWMKSSLLDRNPFSGTWIVGDV 679
                                                                       
Sbjct: 602 ------------------------------------------------------------ 661

Query: 680 KNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQYYEELKSSGVQVTK 739
           K  +  L VLK+M+ A VKPDS+TFSYLIN C  E  I K      YY+E+K +GV+V K
Sbjct: 662 KKVNSALKVLKEMKEADVKPDSVTFSYLINYCGEEATIAK------YYKEMKQAGVEVNK 721

Query: 740 QIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVSALASHGQTADALKIYDEM 799
            ++M+L+ AYA+ GQFEKAKQV+ D  +P K+ NE++SVL+SALAS+G   +AL IY+EM
Sbjct: 722 HVYMSLVKAYASCGQFEKAKQVLMDLEVPAKDHNELKSVLISALASNGNITEALSIYEEM 781

Query: 800 KQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDWIDCCHRIILFSIKHNDLS 859
           K+  C +EPKA++SL+E+   +  L  +++L  +L  D   WID   +II+F++++N  S
Sbjct: 782 KKLRCPVEPKAILSLIENSDSNAELGTLVELTHEL-RDSKFWIDGFFKIIVFAVRNNRSS 836

Query: 860 STVDLLKQLSYRCSNDEVTMGVAFDEIFSLIAESEPTYLETGLQLLQFIKNDLGLSPPRR 919
           S +DLL+Q     S D+V +   F+E+F  IAE+E + ++ GL L+ F+K +L L P R+
Sbjct: 842 SILDLLEQTKNHLSKDDVGVEYWFEEVFKSIAETESSDVKVGLDLVSFMKEELELCPSRK 836

Query: 920 CLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQALLASGALESAKVLLDKI 979
           CLDFLL AC NAKD +S+ L+W+EY+ A LPYN I+YLRMYQ L+A+G  +SA+ ++ KI
Sbjct: 902 CLDFLLHACVNAKDKQSALLVWEEYQCAELPYNVINYLRMYQVLVAAGDSKSAEAIVSKI 836

Query: 980 PKDDAHVCWIIKECEMVYIASPTAKKKRRKKMLK 981
           P DD  V  IIKE  +V+  +P  KKK++K   K
Sbjct: 962 PNDDKDVKCIIKESRIVF--TPKLKKKKKKSKQK 836

BLAST of Lsi04G012400 vs. TAIR 10
Match: AT5G27110.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 6.5e-07
Identity = 41/136 (30.15%), Postives = 71/136 (52.21%), Query Frame = 0

Query: 646 VKNTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQYYEELKSSGVQVT 705
           V N    + V  QM   GVKPD +TF+ ++  C     + K     Q +  +  S ++  
Sbjct: 388 VGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAALEKG---KQIHLSISESRLETD 447

Query: 706 KQIFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVRSVLVSALASHGQTADALKIYDE 765
           + +  AL++ Y+  G  ++A ++ +   IP K++    +V++SA  SHGQ  +AL  +DE
Sbjct: 448 ELLLSALLDMYSKCGNEKEAFRIFN--SIPKKDVVS-WTVMISAYGSHGQPREALYQFDE 507

Query: 766 MKQAGCDLEPKAVISL 782
           M++ G  L+P  V  L
Sbjct: 508 MQKFG--LKPDGVTLL 515

BLAST of Lsi04G012400 vs. TAIR 10
Match: AT5G42310.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.1e-06
Identity = 39/172 (22.67%), Postives = 83/172 (48.26%), Query Frame = 0

Query: 636 PFSGTWIVGDVKNTSD---GLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQ 695
           P +   ++G     +D    L ++ +M   G + D + +S +I +     + I S    +
Sbjct: 197 PLTYNALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSL-TRSNKIDSVMLLR 256

Query: 696 YYEELKSSGVQVTKQIFMALINAYAAHGQFEKAKQVI---SDEGIPVKNLNEVRSVLVSA 755
            Y+E++   +++  Q+   +I  +A  G   KA Q++      G+  K    V   ++SA
Sbjct: 257 LYKEIERDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTATLVS--IISA 316

Query: 756 LASHGQTADALKIYDEMKQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDL 802
           LA  G+T +A  +++E++Q+G     +A  +L++ Y   GPL     ++ ++
Sbjct: 317 LADSGRTLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEM 365

BLAST of Lsi04G012400 vs. TAIR 10
Match: AT1G62590.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.9e-06
Identity = 56/298 (18.79%), Postives = 127/298 (42.62%), Query Frame = 0

Query: 648 NTSDGLMVLKQMELAGVKPDSMTFSYLINNCECEEDIIKSPFFPQYYEELKSSGVQVTKQ 707
           +T   L +L +ME A ++ D + F+ +I++  C+   +        ++E+++ G++    
Sbjct: 240 DTDLALNLLNKMEAAKIEADVVIFNTIIDSL-CKYRHVDDAL--NLFKEMETKGIRPNVV 299

Query: 708 IFMALINAYAAHGQFEKAKQVISDEGIPVKNLNEVR-SVLVSALASHGQTADALKIYDEM 767
            + +LI+   ++G++  A Q++SD      N N V  + L+ A    G+  +A K+YD+M
Sbjct: 300 TYSSLISCLCSYGRWSDASQLLSDMIEKKINPNLVTFNALIDAFVKEGKFVEAEKLYDDM 359

Query: 768 KQAGCDLEPKAVISLVEHYPFDGPLNRMLQLLGDLHHDLDDWIDCCHRIILFS------I 827
            +   D +     SLV  +     L++  Q+   +        DC   ++ ++       
Sbjct: 360 IKRSIDPDIFTYNSLVNGFCMHDRLDKAKQMFEFMVSK-----DCFPDVVTYNTLIKGFC 419

Query: 828 KHNDLSSTVDLLKQLSYR-CSNDEVTMGVAFDEIFSLIAESEPTYLETGLQLLQFIKNDL 887
           K   +    +L +++S+R    D VT       +F           +   ++ + + +D 
Sbjct: 420 KSKRVEDGTELFREMSHRGLVGDTVTYTTLIQGLF------HDGDCDNAQKVFKQMVSD- 479

Query: 888 GLSPPRRCLDFLLGACANAKDAESSRLIWKEYENAGLPYNTISYLRMYQALLASGALE 938
           G+ P       LL    N    E +  ++   + + +  +   Y  M + +  +G ++
Sbjct: 480 GVPPDIMTYSILLDGLCNNGKLEKALEVFDYMQKSEIKLDIYIYTTMIEGMCKAGKVD 522