Lsi04G011530 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi04G011530 vs. ExPASy Swiss-Prot
Match: F4INA9 (ATP-dependent DNA helicase homolog RECG, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RECG PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 1.7e-210
Identity = 415/844 (49.17%), Postives = 525/844 (62.20%), Query Frame = 0

Query: 20  SHYVLSMLPKLCLRTTHKFAGHLFE-VGKYGTASISNRPKLLHKISVVMAHDDCIENAQY 79
           S++  S +  +  R+ HK++ +L E V KY +A + N+ KL+ K++ +M  D        
Sbjct: 37  SNFFFSKVWNISYRSKHKYSDNLLEQVEKYASARLENQSKLITKVAALMECD-------- 96

Query: 80  NNQSNSIPADPDDDC--NVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGEN 139
            N  + I    D+    ++  ACK+FPSIILG S P+EL+  S  Y + +S L   +  +
Sbjct: 97  -NVDDFIDKKSDEQVKKDLVLACKRFPSIILGDSRPVELYSNSKSYGESSSILKTPTDNS 156

Query: 140 FLLNSTCEEWVQ-DSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSN 199
           FL       W   D+L  TLSS    L     SS   E  L+  SS      +T T    
Sbjct: 157 FLPTPMHGGWFDPDNLSRTLSSFCPELLQNDDSSDPREDILDDGSS---FTSKTAT---- 216

Query: 200 PAVEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPR 259
                  S+ E  S ++  + + FL   +  + GLSKRH  QLD  GFHT+ KLLHHFPR
Sbjct: 217 -------SEVEATS-DDVFAAQRFLATSIDSMPGLSKRHSNQLDSCGFHTMKKLLHHFPR 276

Query: 260 TYADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDN 319
           TYADLQN QV+I+DGQYLIF+GKVLSS+G+RAS SFSFLEV+V CE++ R+        N
Sbjct: 277 TYADLQNAQVDIEDGQYLIFVGKVLSSKGVRASSSFSFLEVIVSCEVSGRDRTPEDLSHN 336

Query: 320 --NTGGKKIYLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMRE 379
             +  GK I+LHLKKFFRGTRFT+QPFL S+ EKHK G++VC+SGKV+++++EDH+EMRE
Sbjct: 337 AEDKAGKSIFLHLKKFFRGTRFTWQPFLNSIQEKHKVGDLVCISGKVKSLRAEDHFEMRE 396

Query: 380 YNIDVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTAS 439
           YNIDVL+DE++ S  A+ RPYPIYPSK GLNP FL D+I+R+                  
Sbjct: 397 YNIDVLKDEEESSHRAQGRPYPIYPSKGGLNPKFLSDVISRA------------------ 456

Query: 440 DGWQTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQ 499
                                                      +  LP N+DPIP++IT+
Sbjct: 457 -------------------------------------------LRVLPANMDPIPKEITK 516

Query: 500 GFGLLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTI 559
            FGL  L+DAY GIH+PK++ EADLARKR IFDEFFYLQ                     
Sbjct: 517 VFGLPSLNDAYVGIHEPKTLDEADLARKRLIFDEFFYLQ--------------------- 576

Query: 560 IEVVKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGL 619
                                                            LARL+QML+ L
Sbjct: 577 -------------------------------------------------LARLYQMLQSL 636

Query: 620 GTRIEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPI 679
           GT+IEKD LL+K+R+P LN+ Y++EW+ LT+ FLKALPYSLT SQ+ AV+EIIWDLKRP+
Sbjct: 637 GTKIEKDVLLEKFRKPVLNSVYIEEWSTLTKSFLKALPYSLTPSQLSAVSEIIWDLKRPV 696

Query: 680 PMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIE 739
           PMNRLLQGDVGCGKTVVAFLACMEVIG+GYQAAFM PTELLAIQHYE    LLENMEG+ 
Sbjct: 697 PMNRLLQGDVGCGKTVVAFLACMEVIGSGYQAAFMAPTELLAIQHYEQCRDLLENMEGVS 725

Query: 740 NKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFG 799
           +KP++ LLTGSTP+KQSRMIR+ LQ+G  S +IGTHSLI+EK+E+SALRIAVVDEQ RFG
Sbjct: 757 SKPTIGLLTGSTPAKQSRMIRQDLQSGAISFIIGTHSLIAEKIEYSALRIAVVDEQQRFG 725

Query: 800 VVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMS 858
           V+QRGKF+SKL+  S+ SK  +  SD TS  D+SMAPHVLAMSATPIPR+LALALYGD+S
Sbjct: 817 VIQRGKFNSKLYGTSMISKSGSSDSDDTSKADLSMAPHVLAMSATPIPRSLALALYGDIS 725

BLAST of Lsi04G011530 vs. ExPASy Swiss-Prot
Match: Q55681 (ATP-dependent DNA helicase RecG OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=recG PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 9.4e-47
Identity = 127/373 (34.05%), Postives = 176/373 (47.18%), Query Frame = 0

Query: 485 DPIPEDITQGFGLLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELN 544
           DP+P++I + + L+ L  A   IH P++ ++  LAR+R +FDEFFYLQ  F+        
Sbjct: 322 DPLPQEIREKYELIDLQTAIAQIHFPENTEKLSLARRRLVFDEFFYLQLGFL-------- 381

Query: 545 EKDDRALTIIEVVKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLA 604
                                            Q +  +K++++   F            
Sbjct: 382 ---------------------------------QRRYEQKQQQQSAIFT----------- 441

Query: 605 RLFQMLEGLGTRIEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAE 664
                                   PH           L +KF   LP+ LT++Q + V E
Sbjct: 442 ------------------------PH---------GELLEKFSDLLPFRLTQAQQRVVNE 501

Query: 665 IIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLG 724
           I+ DL +P PMNRL+QGDVG GKTVV   A +  +  GYQAA M PTE+LA QHY+ L+ 
Sbjct: 502 ILQDLNKPSPMNRLVQGDVGSGKTVVGVFAILAALQGGYQAALMAPTEVLAEQHYQKLVS 561

Query: 725 LLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIA 784
               +        V LLTGST + + R I   L TG+  L++GTH+LI E V F  L + 
Sbjct: 562 WFNLL-----YLPVELLTGSTKTAKRREIHAQLSTGQLPLLVGTHALIQETVNFQRLGLV 581

Query: 785 VVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTL 844
           V+DEQHRFGV QR K  +K                         APHVL+M+ATPIPRTL
Sbjct: 622 VIDEQHRFGVQQRAKLLAK-----------------------GNAPHVLSMTATPIPRTL 581

Query: 845 ALALYGDMSLTHV 858
           AL L+GD+ ++ +
Sbjct: 682 ALTLHGDLEVSQI 581

BLAST of Lsi04G011530 vs. ExPASy Swiss-Prot
Match: O67837 (ATP-dependent DNA helicase RecG OS=Aquifex aeolicus (strain VF5) OX=224324 GN=recG PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 4.1e-42
Identity = 96/212 (45.28%), Postives = 136/212 (64.15%), Query Frame = 0

Query: 644 QKFLKALPYSLTESQMKAVAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGY 703
           +K ++ LP+ LT +Q +A+ EI+ DL R +PMNRLLQGDVG GKT+VA L  + V+ +GY
Sbjct: 351 RKAIEKLPFKLTRAQERAIKEILEDLSRDVPMNRLLQGDVGSGKTIVAILTSLAVVKSGY 410

Query: 704 QAAFMVPTELLAIQHYEHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETS 763
           Q A MVPTE+LA QHY+    +L++  G+    +VALLTGS    Q + + + ++ G   
Sbjct: 411 QVAVMVPTEILAHQHYKKFSEMLKDY-GV----NVALLTGSLTPSQKKSVYKHVKEGNIH 470

Query: 764 LVIGTHSLISEKVEFSALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSS 823
           +++GTH+LI +KVEF  L   ++DEQHRFGV+QR              K+  E   G   
Sbjct: 471 VLVGTHALIQDKVEFKNLGYVIIDEQHRFGVMQR--------------KLLLEKGKG--- 530

Query: 824 GDISMAPHVLAMSATPIPRTLALALYGDMSLT 856
               + PH L MSATPIPRTLAL++YGD+ ++
Sbjct: 531 ----LYPHCLVMSATPIPRTLALSIYGDLDIS 536

BLAST of Lsi04G011530 vs. ExPASy Swiss-Prot
Match: Q54900 (ATP-dependent DNA helicase RecG OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=recG PE=3 SV=2)

HSP 1 Score: 166.4 bits (420), Expect = 1.4e-39
Identity = 95/207 (45.89%), Postives = 129/207 (62.32%), Query Frame = 0

Query: 649 ALPYSLTESQMKAVAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFM 708
           +LP++LT++Q K++ EI+ D+K    MNRLLQGDVG GKTVVA LA    +  GYQAA M
Sbjct: 248 SLPFALTQAQEKSLQEILTDMKSDHHMNRLLQGDVGSGKTVVAGLAMFAAVTAGYQAALM 307

Query: 709 VPTELLAIQHYEHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGT 768
           VPTE+LA QH+E L  L  N++       +ALLTGS  + + R + E++  GE  L+IGT
Sbjct: 308 VPTEILAEQHFESLQNLFPNLK-------LALLTGSLKAAEKREVLETIAKGEADLIIGT 367

Query: 769 HSLISEKVEFSALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISM 828
           H+LI + VE++ L + ++DEQHRFGV QR              +I  E  D         
Sbjct: 368 HALIQDGVEYARLGLIIIDEQHRFGVGQR--------------RILREKGDN-------- 424

Query: 829 APHVLAMSATPIPRTLALALYGDMSLT 856
            P VL M+ATPIPRTLA+  +GDM ++
Sbjct: 428 -PDVLMMTATPIPRTLAITAFGDMDVS 424

BLAST of Lsi04G011530 vs. ExPASy Swiss-Prot
Match: O34942 (ATP-dependent DNA helicase RecG OS=Bacillus subtilis (strain 168) OX=224308 GN=recG PE=3 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 3.2e-39
Identity = 93/211 (44.08%), Postives = 126/211 (59.72%), Query Frame = 0

Query: 645 KFLKALPYSLTESQMKAVAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQ 704
           +F+K+LP+ LT +Q + + EI  D+  P  MNRLLQGDVG GKT VA +A    I +GYQ
Sbjct: 248 RFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQ 307

Query: 705 AAFMVPTELLAIQHYEHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSL 764
            A MVPTE+LA QH + L+ L E  +      SVALLT S   K+ + + E L  GE  +
Sbjct: 308 GALMVPTEILAEQHADSLVSLFEKWD-----VSVALLTSSVKGKRRKELLERLAAGEIDI 367

Query: 765 VIGTHSLISEKVEFSALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSG 824
           ++GTH+LI ++VEF AL + + DEQHRFGV QR K  +K                     
Sbjct: 368 LVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRNK--------------------- 427

Query: 825 DISMAPHVLAMSATPIPRTLALALYGDMSLT 856
                P VL M+ATPIPRTLA+ ++G+M ++
Sbjct: 428 --GQDPDVLFMTATPIPRTLAITVFGEMDVS 430

HSP 2 Score: 44.7 bits (104), Expect = 6.4e-03
Identity = 17/48 (35.42%), Postives = 30/48 (62.50%), Query Frame = 0

Query: 485 DPIPEDITQGFGLLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQ 533
           DP+PE + + + L   + A   +HQP++ +   LAR+RF+++EF   Q
Sbjct: 173 DPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQ 220

BLAST of Lsi04G011530 vs. ExPASy TrEMBL
Match: A0A1S3C041 (DNA helicase OS=Cucumis melo OX=3656 GN=LOC103495086 PE=3 SV=1)

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 649/858 (75.64%), Postives = 680/858 (79.25%), Query Frame = 0

Query: 1   MAKYQIQTGRLAHHLFCLRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLL 60
           MAKYQIQT RLAHHLFCLRSHYVLSMLPKLCLRT H+FAG+LFE+GKY T SI  RPKLL
Sbjct: 1   MAKYQIQTSRLAHHLFCLRSHYVLSMLPKLCLRTKHEFAGNLFELGKYATTSIPIRPKLL 60

Query: 61  HKISVVMAHDDCIENAQYNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSA 120
            KIS VMAHDDCIEN QYNNQSNSIP+DPD+DC+VS+ACKKFPSI LGSSPP+ELFDGSA
Sbjct: 61  IKISDVMAHDDCIENGQYNNQSNSIPSDPDEDCDVSKACKKFPSIKLGSSPPVELFDGSA 120

Query: 121 CYSDIASFLAAKSGENFLLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADS 180
           C+SDIASFLAAKS +NF LNSTCEE VQD  D TL SLY  LPD+G SSVSEEYTL   S
Sbjct: 121 CHSDIASFLAAKSSKNFYLNSTCEECVQDE-DRTLPSLYPALPDVGNSSVSEEYTLTTGS 180

Query: 181 SLLPMNIETGTILSNPAVEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDD 240
           SLLPMNIETGTI SNPAVEGDSSKK+  S+N AVSGRSFL Q VGCISGLSKRHQRQLDD
Sbjct: 181 SLLPMNIETGTISSNPAVEGDSSKKKSTSENTAVSGRSFLDQSVGCISGLSKRHQRQLDD 240

Query: 241 SGFHTLGKLLHHFPRTYADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC 300
           SGFHTLGKLLHHFPRTYADL+NPQV+IDDGQY+IF+GKVLSSRGIRASYSFSFLEVVV C
Sbjct: 241 SGFHTLGKLLHHFPRTYADLRNPQVHIDDGQYIIFVGKVLSSRGIRASYSFSFLEVVVCC 300

Query: 301 EIAERESNSSCTVDNNTGGKK-IYLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGK 360
           EIAERESNS CTVD+NTGGKK IYLHLKKFFRG RFTF PFLR LGEKHKEGE+VCVSGK
Sbjct: 301 EIAERESNSGCTVDDNTGGKKIIYLHLKKFFRGNRFTFTPFLRRLGEKHKEGEVVCVSGK 360

Query: 361 VRTMQSEDHYEMREYNIDVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDS 420
           VRTMQSEDHYEMREYNIDVLQDEKDV  YAKERPYPIYPSKRG +PTFLRDIIAR     
Sbjct: 361 VRTMQSEDHYEMREYNIDVLQDEKDVPLYAKERPYPIYPSKRGSSPTFLRDIIAR----- 420

Query: 421 SFKHSYRATTQTASDGWQTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEA 480
                                                                   GI+A
Sbjct: 421 --------------------------------------------------------GIDA 480

Query: 481 LPINVDPIPEDITQGFGLLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVL 540
           LPINVDPIPEDITQGFGLLRL DAYNGIH+PKSIKEADLARKRF+FDEFFYLQ       
Sbjct: 481 LPINVDPIPEDITQGFGLLRLRDAYNGIHRPKSIKEADLARKRFMFDEFFYLQ------- 540

Query: 541 VEELNEKDDRALTIIEVVKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNC 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 NGYLARLFQMLEGLGTRIEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQM 660
              LARLFQMLE LGTRIEKDCLLDKYRQPHLNAAYMK+WACLTQKFLKALPYSLT SQM
Sbjct: 601 ---LARLFQMLERLGTRIEKDCLLDKYRQPHLNAAYMKDWACLTQKFLKALPYSLTVSQM 660

Query: 661 KAVAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHY 720
           KA+AEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHY
Sbjct: 661 KAIAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHY 720

Query: 721 EHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFS 780
           EHLLGLLENMEG+ENKPSVALLTGST SKQSRMIRE LQ+GE SLVIGT SLISEKVEFS
Sbjct: 721 EHLLGLLENMEGVENKPSVALLTGSTSSKQSRMIREGLQSGEISLVIGTQSLISEKVEFS 726

Query: 781 ALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATP 840
           ALRIAVVDEQHRFGVVQRGKF SKLFNNS+SSKIAAE+SDGTSSGD SMAPHVLAMSATP
Sbjct: 781 ALRIAVVDEQHRFGVVQRGKFGSKLFNNSISSKIAAEHSDGTSSGDFSMAPHVLAMSATP 726

Query: 841 IPRTLALALYGDMSLTHV 858
           IPRTLALALYG+MSLTH+
Sbjct: 841 IPRTLALALYGEMSLTHI 726

BLAST of Lsi04G011530 vs. ExPASy TrEMBL
Match: A0A6J1FSV3 (DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111446985 PE=3 SV=1)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 652/846 (77.07%), Postives = 672/846 (79.43%), Query Frame = 0

Query: 18  LRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLLHKISVVMAHDDCIENAQ 77
           +R HYVLSMLPKLC RT H FAG+LFEVGKYGT SISNR KLL KISVVMAHDDCIEN Q
Sbjct: 39  MRFHYVLSMLPKLCSRTKHTFAGNLFEVGKYGTTSISNRGKLLDKISVVMAHDDCIENGQ 98

Query: 78  YNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGENF 137
           YNN+SNSIP+DPDDDCNVS ACKKFPSIILGSSPP+ELFDGSACYSDIASFL+AK+GENF
Sbjct: 99  YNNRSNSIPSDPDDDCNVSVACKKFPSIILGSSPPVELFDGSACYSDIASFLSAKTGENF 158

Query: 138 LLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSNPA 197
           LLNSTCEEWVQDSLDGTLSSLYSVLPD+G SSVSEEYT +A SSLLP NIETGTI SNPA
Sbjct: 159 LLNSTCEEWVQDSLDGTLSSLYSVLPDVGNSSVSEEYTYSAGSSLLPKNIETGTISSNPA 218

Query: 198 VEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPRTY 257
           VE D+S+KELK QNNAVSGRSFL Q VG I GLSKRHQRQLD+SGFHTLGKLLHHFPRTY
Sbjct: 219 VEEDTSRKELKLQNNAVSGRSFLDQSVGSIPGLSKRHQRQLDESGFHTLGKLLHHFPRTY 278

Query: 258 ADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDNNT 317
           ADL+NPQVNI DGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNS C  DNNT
Sbjct: 279 ADLRNPQVNIYDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSGCLADNNT 338

Query: 318 GGKKI-YLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 377
           GGKKI YLHLKKFFRGTRFTFQPFLRSLG  HKEGEIVCVSGKVRTMQSEDHYEMREYNI
Sbjct: 339 GGKKIVYLHLKKFFRGTRFTFQPFLRSLGANHKEGEIVCVSGKVRTMQSEDHYEMREYNI 398

Query: 378 DVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTASDGW 437
           DVLQDEKDV FYAKERPYPIYPSK+GL PTFLRDIIAR                      
Sbjct: 399 DVLQDEKDVPFYAKERPYPIYPSKKGLTPTFLRDIIAR---------------------- 458

Query: 438 QTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQGFG 497
                                                  GIEALP+N+DPIPEDITQ FG
Sbjct: 459 ---------------------------------------GIEALPVNIDPIPEDITQSFG 518

Query: 498 LLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTIIEV 557
           LLRLHDAYNGIHQPKSI EADLARKRFIFDEFFYLQ                        
Sbjct: 519 LLRLHDAYNGIHQPKSINEADLARKRFIFDEFFYLQ------------------------ 578

Query: 558 VKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGLGTR 617
                                                         LARLFQMLEGLGT 
Sbjct: 579 ----------------------------------------------LARLFQMLEGLGTT 638

Query: 618 IEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 677
           IEKD LLDKYRQP LNAAYMKEW CLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN
Sbjct: 639 IEKDGLLDKYRQPRLNAAYMKEWTCLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 698

Query: 678 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIENKP 737
           RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENME IENKP
Sbjct: 699 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEEIENKP 752

Query: 738 SVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFGVVQ 797
           SVALLTGSTPSKQSRMIR+SLQTGETSLVIGTHSLISEKVEFSALR+AVVDEQHRFGVVQ
Sbjct: 759 SVALLTGSTPSKQSRMIRQSLQTGETSLVIGTHSLISEKVEFSALRLAVVDEQHRFGVVQ 752

Query: 798 RGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMSLTH 857
           RGKFSSKLF NSLSSKIAAE +D TSSGD SMAPHVLAMSATPIPRTLALALYGDMSLTH
Sbjct: 819 RGKFSSKLF-NSLSSKIAAETADATSSGDFSMAPHVLAMSATPIPRTLALALYGDMSLTH 752

Query: 858 V--CSP 861
           +  C P
Sbjct: 879 ITDCPP 752

BLAST of Lsi04G011530 vs. ExPASy TrEMBL
Match: A0A6J1FP75 (DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111446985 PE=3 SV=1)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 652/846 (77.07%), Postives = 672/846 (79.43%), Query Frame = 0

Query: 18  LRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLLHKISVVMAHDDCIENAQ 77
           +R HYVLSMLPKLC RT H FAG+LFEVGKYGT SISNR KLL KISVVMAHDDCIEN Q
Sbjct: 20  MRFHYVLSMLPKLCSRTKHTFAGNLFEVGKYGTTSISNRGKLLDKISVVMAHDDCIENGQ 79

Query: 78  YNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGENF 137
           YNN+SNSIP+DPDDDCNVS ACKKFPSIILGSSPP+ELFDGSACYSDIASFL+AK+GENF
Sbjct: 80  YNNRSNSIPSDPDDDCNVSVACKKFPSIILGSSPPVELFDGSACYSDIASFLSAKTGENF 139

Query: 138 LLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSNPA 197
           LLNSTCEEWVQDSLDGTLSSLYSVLPD+G SSVSEEYT +A SSLLP NIETGTI SNPA
Sbjct: 140 LLNSTCEEWVQDSLDGTLSSLYSVLPDVGNSSVSEEYTYSAGSSLLPKNIETGTISSNPA 199

Query: 198 VEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPRTY 257
           VE D+S+KELK QNNAVSGRSFL Q VG I GLSKRHQRQLD+SGFHTLGKLLHHFPRTY
Sbjct: 200 VEEDTSRKELKLQNNAVSGRSFLDQSVGSIPGLSKRHQRQLDESGFHTLGKLLHHFPRTY 259

Query: 258 ADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDNNT 317
           ADL+NPQVNI DGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNS C  DNNT
Sbjct: 260 ADLRNPQVNIYDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSGCLADNNT 319

Query: 318 GGKKI-YLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 377
           GGKKI YLHLKKFFRGTRFTFQPFLRSLG  HKEGEIVCVSGKVRTMQSEDHYEMREYNI
Sbjct: 320 GGKKIVYLHLKKFFRGTRFTFQPFLRSLGANHKEGEIVCVSGKVRTMQSEDHYEMREYNI 379

Query: 378 DVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTASDGW 437
           DVLQDEKDV FYAKERPYPIYPSK+GL PTFLRDIIAR                      
Sbjct: 380 DVLQDEKDVPFYAKERPYPIYPSKKGLTPTFLRDIIAR---------------------- 439

Query: 438 QTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQGFG 497
                                                  GIEALP+N+DPIPEDITQ FG
Sbjct: 440 ---------------------------------------GIEALPVNIDPIPEDITQSFG 499

Query: 498 LLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTIIEV 557
           LLRLHDAYNGIHQPKSI EADLARKRFIFDEFFYLQ                        
Sbjct: 500 LLRLHDAYNGIHQPKSINEADLARKRFIFDEFFYLQ------------------------ 559

Query: 558 VKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGLGTR 617
                                                         LARLFQMLEGLGT 
Sbjct: 560 ----------------------------------------------LARLFQMLEGLGTT 619

Query: 618 IEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 677
           IEKD LLDKYRQP LNAAYMKEW CLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN
Sbjct: 620 IEKDGLLDKYRQPRLNAAYMKEWTCLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 679

Query: 678 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIENKP 737
           RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENME IENKP
Sbjct: 680 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEEIENKP 733

Query: 738 SVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFGVVQ 797
           SVALLTGSTPSKQSRMIR+SLQTGETSLVIGTHSLISEKVEFSALR+AVVDEQHRFGVVQ
Sbjct: 740 SVALLTGSTPSKQSRMIRQSLQTGETSLVIGTHSLISEKVEFSALRLAVVDEQHRFGVVQ 733

Query: 798 RGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMSLTH 857
           RGKFSSKLF NSLSSKIAAE +D TSSGD SMAPHVLAMSATPIPRTLALALYGDMSLTH
Sbjct: 800 RGKFSSKLF-NSLSSKIAAETADATSSGDFSMAPHVLAMSATPIPRTLALALYGDMSLTH 733

Query: 858 V--CSP 861
           +  C P
Sbjct: 860 ITDCPP 733

BLAST of Lsi04G011530 vs. ExPASy TrEMBL
Match: A0A6J1IFJ2 (DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111472714 PE=3 SV=1)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 651/846 (76.95%), Postives = 670/846 (79.20%), Query Frame = 0

Query: 18  LRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLLHKISVVMAHDDCIENAQ 77
           +R H+VLSMLPKLC RT H FAG LFEVGKYGT SISNR KLL KISVVMAHDD IEN Q
Sbjct: 39  MRFHHVLSMLPKLCSRTKHTFAGDLFEVGKYGTTSISNRGKLLDKISVVMAHDDYIENGQ 98

Query: 78  YNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGENF 137
           YNNQSNSIP+D DDDCNVS ACKKFPSIILGSSPP+ELFDGSACYSDIASFL+AK+GENF
Sbjct: 99  YNNQSNSIPSDTDDDCNVSVACKKFPSIILGSSPPVELFDGSACYSDIASFLSAKTGENF 158

Query: 138 LLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSNPA 197
           LLNSTCEEWVQDSLDGTLSSLYSVLPD+G SSVSEEYT +A SSLLP NIETGTI SNPA
Sbjct: 159 LLNSTCEEWVQDSLDGTLSSLYSVLPDVGNSSVSEEYTYSAGSSLLPKNIETGTISSNPA 218

Query: 198 VEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPRTY 257
           VE DSS+K LKSQNNAVSGRSFL Q VGCI GLSKRHQRQLD+SGFHTLGKLLHHFPRTY
Sbjct: 219 VEEDSSRKALKSQNNAVSGRSFLDQSVGCIPGLSKRHQRQLDESGFHTLGKLLHHFPRTY 278

Query: 258 ADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDNNT 317
           ADL+NPQVNI DGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNS C  DNNT
Sbjct: 279 ADLRNPQVNIYDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSGCLADNNT 338

Query: 318 GGKKI-YLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 377
           GGKKI YLHLKKFFRGTRFTFQPFLRSLG KHKEGEIVCVSGKVRTMQSEDHYEMREYNI
Sbjct: 339 GGKKIVYLHLKKFFRGTRFTFQPFLRSLGAKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 398

Query: 378 DVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTASDGW 437
           DVLQDEKDV FYAKERPYPIYPSK+GL PTFLRDIIAR                      
Sbjct: 399 DVLQDEKDVPFYAKERPYPIYPSKKGLQPTFLRDIIAR---------------------- 458

Query: 438 QTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQGFG 497
                                                  GIEALP+N+DPIPEDITQ FG
Sbjct: 459 ---------------------------------------GIEALPVNIDPIPEDITQSFG 518

Query: 498 LLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTIIEV 557
           LLRLHDAYNGIHQPKSI EADLARKRFIFDEFFYLQ                        
Sbjct: 519 LLRLHDAYNGIHQPKSINEADLARKRFIFDEFFYLQ------------------------ 578

Query: 558 VKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGLGTR 617
                                                         LARLFQMLEGLGT 
Sbjct: 579 ----------------------------------------------LARLFQMLEGLGTT 638

Query: 618 IEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 677
           IEKD LLDKYRQP LNAAYMKEW CLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN
Sbjct: 639 IEKDSLLDKYRQPRLNAAYMKEWTCLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 698

Query: 678 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIENKP 737
           RLLQGDVGCGKTVVAFLACMEVIG GYQAAFMVPTELLAIQHYEHLLGLLENME IENKP
Sbjct: 699 RLLQGDVGCGKTVVAFLACMEVIGIGYQAAFMVPTELLAIQHYEHLLGLLENMEEIENKP 752

Query: 738 SVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFGVVQ 797
           SVALLTGSTPSKQSRMIR+SLQTGETSLVIGTHSLISEKVEFSALR+AVVDEQHRFGVVQ
Sbjct: 759 SVALLTGSTPSKQSRMIRQSLQTGETSLVIGTHSLISEKVEFSALRLAVVDEQHRFGVVQ 752

Query: 798 RGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMSLTH 857
           RGKFSSKLF NSLSSKIAAE +D TSSGD SMAPHVLAMSATPIPR+LALALYGDMSLTH
Sbjct: 819 RGKFSSKLF-NSLSSKIAAETADATSSGDFSMAPHVLAMSATPIPRSLALALYGDMSLTH 752

Query: 858 V--CSP 861
           +  C P
Sbjct: 879 ITDCPP 752

BLAST of Lsi04G011530 vs. ExPASy TrEMBL
Match: A0A6J1IDF6 (DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111472714 PE=3 SV=1)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 651/846 (76.95%), Postives = 670/846 (79.20%), Query Frame = 0

Query: 18  LRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLLHKISVVMAHDDCIENAQ 77
           +R H+VLSMLPKLC RT H FAG LFEVGKYGT SISNR KLL KISVVMAHDD IEN Q
Sbjct: 20  MRFHHVLSMLPKLCSRTKHTFAGDLFEVGKYGTTSISNRGKLLDKISVVMAHDDYIENGQ 79

Query: 78  YNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGENF 137
           YNNQSNSIP+D DDDCNVS ACKKFPSIILGSSPP+ELFDGSACYSDIASFL+AK+GENF
Sbjct: 80  YNNQSNSIPSDTDDDCNVSVACKKFPSIILGSSPPVELFDGSACYSDIASFLSAKTGENF 139

Query: 138 LLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSNPA 197
           LLNSTCEEWVQDSLDGTLSSLYSVLPD+G SSVSEEYT +A SSLLP NIETGTI SNPA
Sbjct: 140 LLNSTCEEWVQDSLDGTLSSLYSVLPDVGNSSVSEEYTYSAGSSLLPKNIETGTISSNPA 199

Query: 198 VEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPRTY 257
           VE DSS+K LKSQNNAVSGRSFL Q VGCI GLSKRHQRQLD+SGFHTLGKLLHHFPRTY
Sbjct: 200 VEEDSSRKALKSQNNAVSGRSFLDQSVGCIPGLSKRHQRQLDESGFHTLGKLLHHFPRTY 259

Query: 258 ADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDNNT 317
           ADL+NPQVNI DGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNS C  DNNT
Sbjct: 260 ADLRNPQVNIYDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSGCLADNNT 319

Query: 318 GGKKI-YLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 377
           GGKKI YLHLKKFFRGTRFTFQPFLRSLG KHKEGEIVCVSGKVRTMQSEDHYEMREYNI
Sbjct: 320 GGKKIVYLHLKKFFRGTRFTFQPFLRSLGAKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 379

Query: 378 DVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTASDGW 437
           DVLQDEKDV FYAKERPYPIYPSK+GL PTFLRDIIAR                      
Sbjct: 380 DVLQDEKDVPFYAKERPYPIYPSKKGLQPTFLRDIIAR---------------------- 439

Query: 438 QTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQGFG 497
                                                  GIEALP+N+DPIPEDITQ FG
Sbjct: 440 ---------------------------------------GIEALPVNIDPIPEDITQSFG 499

Query: 498 LLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTIIEV 557
           LLRLHDAYNGIHQPKSI EADLARKRFIFDEFFYLQ                        
Sbjct: 500 LLRLHDAYNGIHQPKSINEADLARKRFIFDEFFYLQ------------------------ 559

Query: 558 VKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGLGTR 617
                                                         LARLFQMLEGLGT 
Sbjct: 560 ----------------------------------------------LARLFQMLEGLGTT 619

Query: 618 IEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 677
           IEKD LLDKYRQP LNAAYMKEW CLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN
Sbjct: 620 IEKDSLLDKYRQPRLNAAYMKEWTCLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 679

Query: 678 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIENKP 737
           RLLQGDVGCGKTVVAFLACMEVIG GYQAAFMVPTELLAIQHYEHLLGLLENME IENKP
Sbjct: 680 RLLQGDVGCGKTVVAFLACMEVIGIGYQAAFMVPTELLAIQHYEHLLGLLENMEEIENKP 733

Query: 738 SVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFGVVQ 797
           SVALLTGSTPSKQSRMIR+SLQTGETSLVIGTHSLISEKVEFSALR+AVVDEQHRFGVVQ
Sbjct: 740 SVALLTGSTPSKQSRMIRQSLQTGETSLVIGTHSLISEKVEFSALRLAVVDEQHRFGVVQ 733

Query: 798 RGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMSLTH 857
           RGKFSSKLF NSLSSKIAAE +D TSSGD SMAPHVLAMSATPIPR+LALALYGDMSLTH
Sbjct: 800 RGKFSSKLF-NSLSSKIAAETADATSSGDFSMAPHVLAMSATPIPRSLALALYGDMSLTH 733

Query: 858 V--CSP 861
           +  C P
Sbjct: 860 ITDCPP 733

BLAST of Lsi04G011530 vs. NCBI nr
Match: XP_038892968.1 (ATP-dependent DNA helicase homolog RECG, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1248.8 bits (3230), Expect = 0.0e+00
Identity = 664/858 (77.39%), Postives = 686/858 (79.95%), Query Frame = 0

Query: 1   MAKYQIQTGRLAHHLFCLRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLL 60
           MAKYQIQTGRLAHHLFCLRSHY+LSMLPK CLRTTHKFAG+LFEVGKY TASISNRP+LL
Sbjct: 20  MAKYQIQTGRLAHHLFCLRSHYLLSMLPKSCLRTTHKFAGNLFEVGKYVTASISNRPRLL 79

Query: 61  HKISVVMAHDDCIENAQYNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSA 120
           HKIS VMAHD CIEN +YNNQSNSIP+D DDDC VS ACKKFPSIILGSSPP+ELFDGS+
Sbjct: 80  HKIS-VMAHDGCIENGEYNNQSNSIPSDQDDDCIVSIACKKFPSIILGSSPPVELFDGSS 139

Query: 121 CYSDIASFLAAKSGENFLLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADS 180
           CYSDIAS LAAKSGENFLLNSTCEE VQDSLDGTLSSLYSVLPD+G SSVSEEY LNA S
Sbjct: 140 CYSDIASLLAAKSGENFLLNSTCEELVQDSLDGTLSSLYSVLPDVGNSSVSEEYNLNAGS 199

Query: 181 SLLPMNIETGTILSNPAVEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDD 240
           SLLPMNIETGTILSN  VEGDSSK ELKSQNNAVS RSFL QPVGCISGLSKRHQRQLDD
Sbjct: 200 SLLPMNIETGTILSNSVVEGDSSKTELKSQNNAVSSRSFLDQPVGCISGLSKRHQRQLDD 259

Query: 241 SGFHTLGKLLHHFPRTYADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC 300
           SGFHTLGKLLHHFPRTYADL+NPQV IDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC
Sbjct: 260 SGFHTLGKLLHHFPRTYADLRNPQVKIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC 319

Query: 301 EIAERESNSSCTVDNNTGGKK-IYLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGK 360
           EIAERESNS C  ++NTGGKK IYLHLKKFFRG+RFTFQPFLRSLGEKHKEGEIVCVSGK
Sbjct: 320 EIAERESNSGCMTNDNTGGKKIIYLHLKKFFRGSRFTFQPFLRSLGEKHKEGEIVCVSGK 379

Query: 361 VRTMQSEDHYEMREYNIDVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDS 420
           VRTMQSEDHYEMREY IDVLQD+KDVSFYAKERPYPIYPSK+GL PTFLRDII R     
Sbjct: 380 VRTMQSEDHYEMREYIIDVLQDKKDVSFYAKERPYPIYPSKKGLKPTFLRDIITR----- 439

Query: 421 SFKHSYRATTQTASDGWQTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEA 480
                                                                   GI+A
Sbjct: 440 --------------------------------------------------------GIDA 499

Query: 481 LPINVDPIPEDITQGFGLLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVL 540
           LP NVDPIPEDITQ FGLLRL+DAY GIHQPKSIKEADLARKRF+FDEFFYLQ       
Sbjct: 500 LPNNVDPIPEDITQDFGLLRLYDAYIGIHQPKSIKEADLARKRFMFDEFFYLQ------- 559

Query: 541 VEELNEKDDRALTIIEVVKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNC 600
                                                                       
Sbjct: 560 ------------------------------------------------------------ 619

Query: 601 NGYLARLFQMLEGLGTRIEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQM 660
              LARLFQMLEGLGTRIEKD LLDKYR+PHLNAAYMKEWA LTQ+FLK LPYSLTESQM
Sbjct: 620 ---LARLFQMLEGLGTRIEKDRLLDKYRKPHLNAAYMKEWAPLTQEFLKVLPYSLTESQM 679

Query: 661 KAVAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHY 720
           KAV EIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTG QAAFMVPTELLAIQHY
Sbjct: 680 KAVTEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGCQAAFMVPTELLAIQHY 739

Query: 721 EHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFS 780
           EHLLGLLE+MEGIENKPSVALLTGST SKQSRMIRESLQTGETSLVIGT SLISEKVEFS
Sbjct: 740 EHLLGLLESMEGIENKPSVALLTGSTSSKQSRMIRESLQTGETSLVIGTQSLISEKVEFS 745

Query: 781 ALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATP 840
           ALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKI  E SDGTSSGD  MAPHVLAMSATP
Sbjct: 800 ALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKITVECSDGTSSGDFYMAPHVLAMSATP 745

Query: 841 IPRTLALALYGDMSLTHV 858
           IPRTLALALYGD+SLTH+
Sbjct: 860 IPRTLALALYGDVSLTHI 745

BLAST of Lsi04G011530 vs. NCBI nr
Match: XP_038892967.1 (ATP-dependent DNA helicase homolog RECG, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1244.2 bits (3218), Expect = 0.0e+00
Identity = 664/859 (77.30%), Postives = 686/859 (79.86%), Query Frame = 0

Query: 1   MAKYQIQTGRLAHHLFCLRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLL 60
           MAKYQIQTGRLAHHLFCLRSHY+LSMLPK CLRTTHKFAG+LFEVGKY TASISNRP+LL
Sbjct: 20  MAKYQIQTGRLAHHLFCLRSHYLLSMLPKSCLRTTHKFAGNLFEVGKYVTASISNRPRLL 79

Query: 61  HKISVVMAHDDCIENAQYNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSA 120
           HKIS VMAHD CIEN +YNNQSNSIP+D DDDC VS ACKKFPSIILGSSPP+ELFDGS+
Sbjct: 80  HKIS-VMAHDGCIENGEYNNQSNSIPSDQDDDCIVSIACKKFPSIILGSSPPVELFDGSS 139

Query: 121 CYSDIASFLAAKSGENFLLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADS 180
           CYSDIAS LAAKSGENFLLNSTCEE VQDSLDGTLSSLYSVLPD+G SSVSEEY LNA S
Sbjct: 140 CYSDIASLLAAKSGENFLLNSTCEELVQDSLDGTLSSLYSVLPDVGNSSVSEEYNLNAGS 199

Query: 181 SLLPMNIETGTILSNPAVEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDD 240
           SLLPMNIETGTILSN  VEGDSSK ELKSQNNAVS RSFL QPVGCISGLSKRHQRQLDD
Sbjct: 200 SLLPMNIETGTILSNSVVEGDSSKTELKSQNNAVSSRSFLDQPVGCISGLSKRHQRQLDD 259

Query: 241 SGFHTLGKLLHHFPRTYADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC 300
           SGFHTLGKLLHHFPRTYADL+NPQV IDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC
Sbjct: 260 SGFHTLGKLLHHFPRTYADLRNPQVKIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC 319

Query: 301 EIAERESNSSCTVDNNTGGKK-IYLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGK 360
           EIAERESNS C  ++NTGGKK IYLHLKKFFRG+RFTFQPFLRSLGEKHKEGEIVCVSGK
Sbjct: 320 EIAERESNSGCMTNDNTGGKKIIYLHLKKFFRGSRFTFQPFLRSLGEKHKEGEIVCVSGK 379

Query: 361 VRTMQSEDHYEMREYNIDVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDS 420
           VRTMQSEDHYEMREY IDVLQD+KDVSFYAKERPYPIYPSK+GL PTFLRDII R     
Sbjct: 380 VRTMQSEDHYEMREYIIDVLQDKKDVSFYAKERPYPIYPSKKGLKPTFLRDIITR----- 439

Query: 421 SFKHSYRATTQTASDGWQTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEA 480
                                                                   GI+A
Sbjct: 440 --------------------------------------------------------GIDA 499

Query: 481 LPINVDPIPEDITQGFGLLRLHD-AYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICV 540
           LP NVDPIPEDITQ FGLLRL+D AY GIHQPKSIKEADLARKRF+FDEFFYLQ      
Sbjct: 500 LPNNVDPIPEDITQDFGLLRLYDQAYIGIHQPKSIKEADLARKRFMFDEFFYLQ------ 559

Query: 541 LVEELNEKDDRALTIIEVVKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDN 600
                                                                       
Sbjct: 560 ------------------------------------------------------------ 619

Query: 601 CNGYLARLFQMLEGLGTRIEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQ 660
               LARLFQMLEGLGTRIEKD LLDKYR+PHLNAAYMKEWA LTQ+FLK LPYSLTESQ
Sbjct: 620 ----LARLFQMLEGLGTRIEKDRLLDKYRKPHLNAAYMKEWAPLTQEFLKVLPYSLTESQ 679

Query: 661 MKAVAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQH 720
           MKAV EIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTG QAAFMVPTELLAIQH
Sbjct: 680 MKAVTEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGCQAAFMVPTELLAIQH 739

Query: 721 YEHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEF 780
           YEHLLGLLE+MEGIENKPSVALLTGST SKQSRMIRESLQTGETSLVIGT SLISEKVEF
Sbjct: 740 YEHLLGLLESMEGIENKPSVALLTGSTSSKQSRMIRESLQTGETSLVIGTQSLISEKVEF 746

Query: 781 SALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSAT 840
           SALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKI  E SDGTSSGD  MAPHVLAMSAT
Sbjct: 800 SALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKITVECSDGTSSGDFYMAPHVLAMSAT 746

Query: 841 PIPRTLALALYGDMSLTHV 858
           PIPRTLALALYGD+SLTH+
Sbjct: 860 PIPRTLALALYGDVSLTHI 746

BLAST of Lsi04G011530 vs. NCBI nr
Match: KAG7031455.1 (ATP-dependent DNA helicase-like RECG, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 654/846 (77.30%), Postives = 673/846 (79.55%), Query Frame = 0

Query: 18  LRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLLHKISVVMAHDDCIENAQ 77
           +R HYVLSMLPKLC RT H FAG+LFEVGKYGT SISNR KLL KISVVMAHDDCIEN Q
Sbjct: 39  MRFHYVLSMLPKLCSRTKHTFAGNLFEVGKYGTTSISNRGKLLDKISVVMAHDDCIENGQ 98

Query: 78  YNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGENF 137
           YNNQSNSIP+DPDDDCNVS ACKKFPSIILGSSPP+ELFDGSACYSDIASFL+AK+GENF
Sbjct: 99  YNNQSNSIPSDPDDDCNVSVACKKFPSIILGSSPPVELFDGSACYSDIASFLSAKTGENF 158

Query: 138 LLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSNPA 197
           LLNSTCEEWVQDSLDGTLSSLYSVLPD+G SSVSEEY  +A SSLLP NIETGTI SNPA
Sbjct: 159 LLNSTCEEWVQDSLDGTLSSLYSVLPDVGNSSVSEEYAYSAGSSLLPKNIETGTISSNPA 218

Query: 198 VEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPRTY 257
           VE D+S+KELKSQNNAVSGRSFL Q VGCI GLSKRHQRQLD+SGFHTLGKLLHHFPRTY
Sbjct: 219 VEEDTSRKELKSQNNAVSGRSFLDQSVGCIPGLSKRHQRQLDESGFHTLGKLLHHFPRTY 278

Query: 258 ADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDNNT 317
           ADL+NPQVNI DGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNS C  DNNT
Sbjct: 279 ADLRNPQVNIYDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSGCLADNNT 338

Query: 318 GGKKI-YLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 377
           GGKKI YLHLKKFFRGTRFTFQPFLRSLG  HKEGEIVCVSGKVRTMQSEDHYEMREYNI
Sbjct: 339 GGKKIVYLHLKKFFRGTRFTFQPFLRSLGANHKEGEIVCVSGKVRTMQSEDHYEMREYNI 398

Query: 378 DVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTASDGW 437
           DVLQDEKDV FYAKERPYPIYPSK+GL PTFLRDIIAR                      
Sbjct: 399 DVLQDEKDVPFYAKERPYPIYPSKKGLTPTFLRDIIAR---------------------- 458

Query: 438 QTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQGFG 497
                                                  GIEALP+N+DPIPEDITQ FG
Sbjct: 459 ---------------------------------------GIEALPVNIDPIPEDITQSFG 518

Query: 498 LLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTIIEV 557
           LLRLHDAYNGIHQPKSI EADLARKRFIFDEFFYLQ                        
Sbjct: 519 LLRLHDAYNGIHQPKSINEADLARKRFIFDEFFYLQ------------------------ 578

Query: 558 VKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGLGTR 617
                                                         LARLFQMLEGLGT 
Sbjct: 579 ----------------------------------------------LARLFQMLEGLGTT 638

Query: 618 IEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 677
           IEKD LLDKYRQP LNAAYMKEW CLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN
Sbjct: 639 IEKDGLLDKYRQPRLNAAYMKEWTCLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 698

Query: 678 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIENKP 737
           RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENME IENKP
Sbjct: 699 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEEIENKP 752

Query: 738 SVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFGVVQ 797
           SVALLTGSTPSKQSRMIR+SLQTGETSLVIGTHSLISEKVEFSALR+AVVDEQHRFGVVQ
Sbjct: 759 SVALLTGSTPSKQSRMIRQSLQTGETSLVIGTHSLISEKVEFSALRLAVVDEQHRFGVVQ 752

Query: 798 RGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMSLTH 857
           RGKFSSKLF NSLSSKIAAE +D TSSGD SMAPHVLAMSATPIPRTLALALYGDMSLTH
Sbjct: 819 RGKFSSKLF-NSLSSKIAAETADATSSGDFSMAPHVLAMSATPIPRTLALALYGDMSLTH 752

Query: 858 V--CSP 861
           +  C P
Sbjct: 879 ITDCPP 752

BLAST of Lsi04G011530 vs. NCBI nr
Match: XP_008454766.1 (PREDICTED: ATP-dependent DNA helicase homolog RECG, chloroplastic [Cucumis melo])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 649/858 (75.64%), Postives = 680/858 (79.25%), Query Frame = 0

Query: 1   MAKYQIQTGRLAHHLFCLRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLL 60
           MAKYQIQT RLAHHLFCLRSHYVLSMLPKLCLRT H+FAG+LFE+GKY T SI  RPKLL
Sbjct: 1   MAKYQIQTSRLAHHLFCLRSHYVLSMLPKLCLRTKHEFAGNLFELGKYATTSIPIRPKLL 60

Query: 61  HKISVVMAHDDCIENAQYNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSA 120
            KIS VMAHDDCIEN QYNNQSNSIP+DPD+DC+VS+ACKKFPSI LGSSPP+ELFDGSA
Sbjct: 61  IKISDVMAHDDCIENGQYNNQSNSIPSDPDEDCDVSKACKKFPSIKLGSSPPVELFDGSA 120

Query: 121 CYSDIASFLAAKSGENFLLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADS 180
           C+SDIASFLAAKS +NF LNSTCEE VQD  D TL SLY  LPD+G SSVSEEYTL   S
Sbjct: 121 CHSDIASFLAAKSSKNFYLNSTCEECVQDE-DRTLPSLYPALPDVGNSSVSEEYTLTTGS 180

Query: 181 SLLPMNIETGTILSNPAVEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDD 240
           SLLPMNIETGTI SNPAVEGDSSKK+  S+N AVSGRSFL Q VGCISGLSKRHQRQLDD
Sbjct: 181 SLLPMNIETGTISSNPAVEGDSSKKKSTSENTAVSGRSFLDQSVGCISGLSKRHQRQLDD 240

Query: 241 SGFHTLGKLLHHFPRTYADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGC 300
           SGFHTLGKLLHHFPRTYADL+NPQV+IDDGQY+IF+GKVLSSRGIRASYSFSFLEVVV C
Sbjct: 241 SGFHTLGKLLHHFPRTYADLRNPQVHIDDGQYIIFVGKVLSSRGIRASYSFSFLEVVVCC 300

Query: 301 EIAERESNSSCTVDNNTGGKK-IYLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGK 360
           EIAERESNS CTVD+NTGGKK IYLHLKKFFRG RFTF PFLR LGEKHKEGE+VCVSGK
Sbjct: 301 EIAERESNSGCTVDDNTGGKKIIYLHLKKFFRGNRFTFTPFLRRLGEKHKEGEVVCVSGK 360

Query: 361 VRTMQSEDHYEMREYNIDVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDS 420
           VRTMQSEDHYEMREYNIDVLQDEKDV  YAKERPYPIYPSKRG +PTFLRDIIAR     
Sbjct: 361 VRTMQSEDHYEMREYNIDVLQDEKDVPLYAKERPYPIYPSKRGSSPTFLRDIIAR----- 420

Query: 421 SFKHSYRATTQTASDGWQTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEA 480
                                                                   GI+A
Sbjct: 421 --------------------------------------------------------GIDA 480

Query: 481 LPINVDPIPEDITQGFGLLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVL 540
           LPINVDPIPEDITQGFGLLRL DAYNGIH+PKSIKEADLARKRF+FDEFFYLQ       
Sbjct: 481 LPINVDPIPEDITQGFGLLRLRDAYNGIHRPKSIKEADLARKRFMFDEFFYLQ------- 540

Query: 541 VEELNEKDDRALTIIEVVKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNC 600
                                                                       
Sbjct: 541 ------------------------------------------------------------ 600

Query: 601 NGYLARLFQMLEGLGTRIEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQM 660
              LARLFQMLE LGTRIEKDCLLDKYRQPHLNAAYMK+WACLTQKFLKALPYSLT SQM
Sbjct: 601 ---LARLFQMLERLGTRIEKDCLLDKYRQPHLNAAYMKDWACLTQKFLKALPYSLTVSQM 660

Query: 661 KAVAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHY 720
           KA+AEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHY
Sbjct: 661 KAIAEIIWDLKRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHY 720

Query: 721 EHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFS 780
           EHLLGLLENMEG+ENKPSVALLTGST SKQSRMIRE LQ+GE SLVIGT SLISEKVEFS
Sbjct: 721 EHLLGLLENMEGVENKPSVALLTGSTSSKQSRMIREGLQSGEISLVIGTQSLISEKVEFS 726

Query: 781 ALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATP 840
           ALRIAVVDEQHRFGVVQRGKF SKLFNNS+SSKIAAE+SDGTSSGD SMAPHVLAMSATP
Sbjct: 781 ALRIAVVDEQHRFGVVQRGKFGSKLFNNSISSKIAAEHSDGTSSGDFSMAPHVLAMSATP 726

Query: 841 IPRTLALALYGDMSLTHV 858
           IPRTLALALYG+MSLTH+
Sbjct: 841 IPRTLALALYGEMSLTHI 726

BLAST of Lsi04G011530 vs. NCBI nr
Match: XP_023538011.1 (ATP-dependent DNA helicase homolog RECG, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >XP_023538018.1 ATP-dependent DNA helicase homolog RECG, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 654/846 (77.30%), Postives = 673/846 (79.55%), Query Frame = 0

Query: 18  LRSHYVLSMLPKLCLRTTHKFAGHLFEVGKYGTASISNRPKLLHKISVVMAHDDCIENAQ 77
           +R HYVLSMLPKLC RT H FAG+LFEVGKYGT SISNR KLL KISVVMAHDDCIEN Q
Sbjct: 39  MRFHYVLSMLPKLCSRTKHTFAGNLFEVGKYGTTSISNRGKLLDKISVVMAHDDCIENGQ 98

Query: 78  YNNQSNSIPADPDDDCNVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGENF 137
           YNNQSNSIP+DPDDDCNVS ACKKFPSIILGSSPP+ELFDGSACYSDIASFL+AK+GENF
Sbjct: 99  YNNQSNSIPSDPDDDCNVSVACKKFPSIILGSSPPVELFDGSACYSDIASFLSAKTGENF 158

Query: 138 LLNSTCEEWVQDSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSNPA 197
           LLNSTCEEWVQDSLDGTLSSLYSVLPD+G SSVSEEYT +A SSLLP NIETGTI SNPA
Sbjct: 159 LLNSTCEEWVQDSLDGTLSSLYSVLPDVGNSSVSEEYTYSAGSSLLPKNIETGTISSNPA 218

Query: 198 VEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPRTY 257
           VE D+S+KELKSQNNAVSGRSFL Q VGCI GLSKRHQRQLD+SGFHTLGKLLHHFPRTY
Sbjct: 219 VEEDTSRKELKSQNNAVSGRSFLDQSVGCIPGLSKRHQRQLDESGFHTLGKLLHHFPRTY 278

Query: 258 ADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDNNT 317
           ADL+NPQVNI DGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNS C  DNNT
Sbjct: 279 ADLRNPQVNIYDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSGCLADNNT 338

Query: 318 GGKKI-YLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMREYNI 377
           GGKKI YLHLKKFFRGTRFTFQPFLRSLG  HKEGEIVCVSGKVRTMQSEDHYEMREYNI
Sbjct: 339 GGKKIVYLHLKKFFRGTRFTFQPFLRSLGANHKEGEIVCVSGKVRTMQSEDHYEMREYNI 398

Query: 378 DVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTASDGW 437
           DVLQDEKDV FYAKERPYPIYPSK+GL PTFL DIIAR                      
Sbjct: 399 DVLQDEKDVPFYAKERPYPIYPSKKGLTPTFLGDIIAR---------------------- 458

Query: 438 QTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQGFG 497
                                                  GIEALP+N+DPIPEDITQ FG
Sbjct: 459 ---------------------------------------GIEALPVNIDPIPEDITQSFG 518

Query: 498 LLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTIIEV 557
           LLRLHDAYNGIHQPKSI EADLARKRFIFDEFFYLQ                        
Sbjct: 519 LLRLHDAYNGIHQPKSINEADLARKRFIFDEFFYLQ------------------------ 578

Query: 558 VKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGLGTR 617
                                                         LARLFQMLEGLGT 
Sbjct: 579 ----------------------------------------------LARLFQMLEGLGTT 638

Query: 618 IEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 677
           IEKD LLDKYRQP LNAAYMKEW CLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN
Sbjct: 639 IEKDGLLDKYRQPRLNAAYMKEWTCLTQKFLKALPYSLTESQMKAVAEIIWDLKRPIPMN 698

Query: 678 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIENKP 737
           RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENME IENKP
Sbjct: 699 RLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEEIENKP 752

Query: 738 SVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFGVVQ 797
           SVALLTGSTPSKQSRMIR+SLQTGETSLVIGTHSLISEKVEFSALR+AVVDEQHRFGVVQ
Sbjct: 759 SVALLTGSTPSKQSRMIRQSLQTGETSLVIGTHSLISEKVEFSALRLAVVDEQHRFGVVQ 752

Query: 798 RGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMSLTH 857
           RGKFSSKLF NSLSSKIAAE +D TSSGD SMAPHVLAMSATPIPRTLALALYGDMSLTH
Sbjct: 819 RGKFSSKLF-NSLSSKIAAETADATSSGDFSMAPHVLAMSATPIPRTLALALYGDMSLTH 752

Query: 858 V--CSP 861
           +  C P
Sbjct: 879 ITDCPP 752

BLAST of Lsi04G011530 vs. TAIR 10
Match: AT2G01440.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 734.2 bits (1894), Expect = 1.2e-211
Identity = 415/844 (49.17%), Postives = 525/844 (62.20%), Query Frame = 0

Query: 20  SHYVLSMLPKLCLRTTHKFAGHLFE-VGKYGTASISNRPKLLHKISVVMAHDDCIENAQY 79
           S++  S +  +  R+ HK++ +L E V KY +A + N+ KL+ K++ +M  D        
Sbjct: 37  SNFFFSKVWNISYRSKHKYSDNLLEQVEKYASARLENQSKLITKVAALMECD-------- 96

Query: 80  NNQSNSIPADPDDDC--NVSRACKKFPSIILGSSPPIELFDGSACYSDIASFLAAKSGEN 139
            N  + I    D+    ++  ACK+FPSIILG S P+EL+  S  Y + +S L   +  +
Sbjct: 97  -NVDDFIDKKSDEQVKKDLVLACKRFPSIILGDSRPVELYSNSKSYGESSSILKTPTDNS 156

Query: 140 FLLNSTCEEWVQ-DSLDGTLSSLYSVLPDLGKSSVSEEYTLNADSSLLPMNIETGTILSN 199
           FL       W   D+L  TLSS    L     SS   E  L+  SS      +T T    
Sbjct: 157 FLPTPMHGGWFDPDNLSRTLSSFCPELLQNDDSSDPREDILDDGSS---FTSKTAT---- 216

Query: 200 PAVEGDSSKKELKSQNNAVSGRSFLYQPVGCISGLSKRHQRQLDDSGFHTLGKLLHHFPR 259
                  S+ E  S ++  + + FL   +  + GLSKRH  QLD  GFHT+ KLLHHFPR
Sbjct: 217 -------SEVEATS-DDVFAAQRFLATSIDSMPGLSKRHSNQLDSCGFHTMKKLLHHFPR 276

Query: 260 TYADLQNPQVNIDDGQYLIFIGKVLSSRGIRASYSFSFLEVVVGCEIAERESNSSCTVDN 319
           TYADLQN QV+I+DGQYLIF+GKVLSS+G+RAS SFSFLEV+V CE++ R+        N
Sbjct: 277 TYADLQNAQVDIEDGQYLIFVGKVLSSKGVRASSSFSFLEVIVSCEVSGRDRTPEDLSHN 336

Query: 320 --NTGGKKIYLHLKKFFRGTRFTFQPFLRSLGEKHKEGEIVCVSGKVRTMQSEDHYEMRE 379
             +  GK I+LHLKKFFRGTRFT+QPFL S+ EKHK G++VC+SGKV+++++EDH+EMRE
Sbjct: 337 AEDKAGKSIFLHLKKFFRGTRFTWQPFLNSIQEKHKVGDLVCISGKVKSLRAEDHFEMRE 396

Query: 380 YNIDVLQDEKDVSFYAKERPYPIYPSKRGLNPTFLRDIIARSSPDSSFKHSYRATTQTAS 439
           YNIDVL+DE++ S  A+ RPYPIYPSK GLNP FL D+I+R+                  
Sbjct: 397 YNIDVLKDEEESSHRAQGRPYPIYPSKGGLNPKFLSDVISRA------------------ 456

Query: 440 DGWQTETEARRVAGGDQCGSTGQWLKNTKLAWGMKDAINPLKGIEALPINVDPIPEDITQ 499
                                                      +  LP N+DPIP++IT+
Sbjct: 457 -------------------------------------------LRVLPANMDPIPKEITK 516

Query: 500 GFGLLRLHDAYNGIHQPKSIKEADLARKRFIFDEFFYLQFSFICVLVEELNEKDDRALTI 559
            FGL  L+DAY GIH+PK++ EADLARKR IFDEFFYLQ                     
Sbjct: 517 VFGLPSLNDAYVGIHEPKTLDEADLARKRLIFDEFFYLQ--------------------- 576

Query: 560 IEVVKIVKNSTIAYPWRDPCYAFNQEKKRKKRKKRGKHFVNSLDNCNGYLARLFQMLEGL 619
                                                            LARL+QML+ L
Sbjct: 577 -------------------------------------------------LARLYQMLQSL 636

Query: 620 GTRIEKDCLLDKYRQPHLNAAYMKEWACLTQKFLKALPYSLTESQMKAVAEIIWDLKRPI 679
           GT+IEKD LL+K+R+P LN+ Y++EW+ LT+ FLKALPYSLT SQ+ AV+EIIWDLKRP+
Sbjct: 637 GTKIEKDVLLEKFRKPVLNSVYIEEWSTLTKSFLKALPYSLTPSQLSAVSEIIWDLKRPV 696

Query: 680 PMNRLLQGDVGCGKTVVAFLACMEVIGTGYQAAFMVPTELLAIQHYEHLLGLLENMEGIE 739
           PMNRLLQGDVGCGKTVVAFLACMEVIG+GYQAAFM PTELLAIQHYE    LLENMEG+ 
Sbjct: 697 PMNRLLQGDVGCGKTVVAFLACMEVIGSGYQAAFMAPTELLAIQHYEQCRDLLENMEGVS 725

Query: 740 NKPSVALLTGSTPSKQSRMIRESLQTGETSLVIGTHSLISEKVEFSALRIAVVDEQHRFG 799
           +KP++ LLTGSTP+KQSRMIR+ LQ+G  S +IGTHSLI+EK+E+SALRIAVVDEQ RFG
Sbjct: 757 SKPTIGLLTGSTPAKQSRMIRQDLQSGAISFIIGTHSLIAEKIEYSALRIAVVDEQQRFG 725

Query: 800 VVQRGKFSSKLFNNSLSSKIAAEYSDGTSSGDISMAPHVLAMSATPIPRTLALALYGDMS 858
           V+QRGKF+SKL+  S+ SK  +  SD TS  D+SMAPHVLAMSATPIPR+LALALYGD+S
Sbjct: 817 VIQRGKFNSKLYGTSMISKSGSSDSDDTSKADLSMAPHVLAMSATPIPRSLALALYGDIS 725

BLAST of Lsi04G011530 vs. TAIR 10
Match: AT3G02060.1 (DEAD/DEAH box helicase, putative )

HSP 1 Score: 114.8 bits (286), Expect = 3.6e-25
Identity = 78/216 (36.11%), Postives = 112/216 (51.85%), Query Frame = 0

Query: 646 FLKALPYSLTESQMKAVAEIIWDL-KRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQ 705
           F    PY+ T  Q +A  ++  DL +R  PM+RL+ GDVG GKT VA  A   V+ TG Q
Sbjct: 262 FAAQFPYNATPDQKQAFLDVEKDLTERETPMDRLICGDVGFGKTEVALRAIFCVVSTGKQ 321

Query: 706 AAFMVPTELLAIQHYEHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSL 765
           A  + PT +LA QHY+    ++     +     V LL+      +     E ++TG  ++
Sbjct: 322 AMVLAPTIVLAKQHYD----VISERFSLYPHIKVGLLSRFQTKAEKEEYLEMIKTGHLNI 381

Query: 766 VIGTHSLISEKVEFSALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSG 825
           ++GTHSL+  +V +S L + VVDE+ RFGV Q+ K +S  F  S+               
Sbjct: 382 IVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIAS--FKTSVD-------------- 441

Query: 826 DISMAPHVLAMSATPIPRTLALALYGDMSLTHVCSP 861
                  VL +SATPIPRTL LAL G    + + +P
Sbjct: 442 -------VLTLSATPIPRTLYLALTGFRDASLISTP 450

BLAST of Lsi04G011530 vs. TAIR 10
Match: AT3G02060.2 (DEAD/DEAH box helicase, putative )

HSP 1 Score: 114.8 bits (286), Expect = 3.6e-25
Identity = 78/216 (36.11%), Postives = 112/216 (51.85%), Query Frame = 0

Query: 646 FLKALPYSLTESQMKAVAEIIWDL-KRPIPMNRLLQGDVGCGKTVVAFLACMEVIGTGYQ 705
           F    PY+ T  Q +A  ++  DL +R  PM+RL+ GDVG GKT VA  A   V+ TG Q
Sbjct: 261 FAAQFPYNATPDQKQAFLDVEKDLTERETPMDRLICGDVGFGKTEVALRAIFCVVSTGKQ 320

Query: 706 AAFMVPTELLAIQHYEHLLGLLENMEGIENKPSVALLTGSTPSKQSRMIRESLQTGETSL 765
           A  + PT +LA QHY+    ++     +     V LL+      +     E ++TG  ++
Sbjct: 321 AMVLAPTIVLAKQHYD----VISERFSLYPHIKVGLLSRFQTKAEKEEYLEMIKTGHLNI 380

Query: 766 VIGTHSLISEKVEFSALRIAVVDEQHRFGVVQRGKFSSKLFNNSLSSKIAAEYSDGTSSG 825
           ++GTHSL+  +V +S L + VVDE+ RFGV Q+ K +S  F  S+               
Sbjct: 381 IVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIAS--FKTSVD-------------- 440

Query: 826 DISMAPHVLAMSATPIPRTLALALYGDMSLTHVCSP 861
                  VL +SATPIPRTL LAL G    + + +P
Sbjct: 441 -------VLTLSATPIPRTLYLALTGFRDASLISTP 449