Lsi04G009910 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G009910
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr04: 10800605 .. 10802296 (+)
RNA-Seq Expression	Lsi04G009910
Synteny	Lsi04G009910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTCTTGAGCTCTTACTTTATATATGATGATTTCTTCTTGGAGACGGCGGAGAGCCACCCGCCGAATGGCAAGGGGTGGTGTCAGCGATGCGGTGGAGTTGACAATCCCGAGTCATTTTCGGTGCCCGATATCGCTCGACTTGATGAAAGATCCGGTGACTTTGTCGACGGGGATAACGTACGATCGAGCTAGCATTGAGACTTGGATTGAAGGAGGGAATTTCAGCTGCCCCTTCACCAACCAGCCATTGCAAAGTATTGATTCTATTCCTAATCATAACATAAGGAAGATGATCCAAGATTGGTGTGTTGAGAATAGAGCTTATGGGATTGAGAGGATTCCGACACCGAGGGTTCCGGCCAGTCCGGCGCAGGTTCGGGAAATTCTTGAGAAGATTTCCAGCGCAGCACGACGGGGGGATTGTGATGGATGCAAGTCCATGGTGGAGAAGATCAAGAAATTGGGGAAAGAAAGTGAAAGGAACAAGAAATGTATTATTGCCAATGGAACAGGTTTGTAAACTATTTTTGCTAAATGCTTTTTAATTTTTTTTTTCATTTGAAACTAGCTCTGATATCCTGTTAAAACAAACCAAAACTTGAAACTTGAGGATTGATATACTTTTATTTATATATATATATTTTTTGAAATGCAGGTAATATATTATCATCAACATTCGAAGCGTTTTCGAAACCCGAAAAATTTGATAGAAGCATCGAGATCTTAGAAGAAATCTTATCAGCCGTGACAATGATGTTCCCTCTACAAGAAGAAGCCACTAAACACTTGAAATCAGAAACTTCATTACAATCATTGGTATGGTTTCTAAAAGGAGGAGATATATCAGGAAGAAGAAATTCAGTTTTGGTATTGAAAGAGATAATTTCATCATACCCAGAAAAAGTTAATGAATTAGGAGAAATCCAAGGAGCATTAGAGGGTCTTATTAAGCTCATAAAAGACCCAATTTGCTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGTCATAGCCTCAACTTCTTCTTCCAATCAAGAGAGATTCACAAAAGCACTTCTTCAAATGGGGTTGGTCTCTTTGCTACTCGAAACCCTCGTCGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGCGTTCGACGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCACGCTCTGACGATGCCTGTGATTGTGAAAAAGATACTTAGAGTATCGGATTTGGCGACCGAGTTATCGGTCTCCATTGTTTGGAAGCTTGTTAAGCATGAGAGTAGAGAAGATGAAGAAGAAGATGGAGGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGCAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGATAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACATTTTGATTGAAAAGTTAATTACTTAAATTCATGAGGATATAATGCAAATAATGTAAGTTGGTTGTGTGGGTGAGGATTTTGTGGCCTTTTCCTCTTTTTTTTTCTTCCCATTTTTATACGATTTCATTGTTGTACAAAGAAATTAAAGTAAAAAACAAATATAGGGATGTCCTTGTTAAACAACTATGT

mRNA sequence

TTTCTCTTGAGCTCTTACTTTATATATGATGATTTCTTCTTGGAGACGGCGGAGAGCCACCCGCCGAATGGCAAGGGGTGGTGTCAGCGATGCGGTGGAGTTGACAATCCCGAGTCATTTTCGGTGCCCGATATCGCTCGACTTGATGAAAGATCCGGTGACTTTGTCGACGGGGATAACGTACGATCGAGCTAGCATTGAGACTTGGATTGAAGGAGGGAATTTCAGCTGCCCCTTCACCAACCAGCCATTGCAAAGTATTGATTCTATTCCTAATCATAACATAAGGAAGATGATCCAAGATTGGTGTGTTGAGAATAGAGCTTATGGGATTGAGAGGATTCCGACACCGAGGGTTCCGGCCAGTCCGGCGCAGGTTCGGGAAATTCTTGAGAAGATTTCCAGCGCAGCACGACGGGGGGATTGTGATGGATGCAAGTCCATGGTGGAGAAGATCAAGAAATTGGGGAAAGAAAGTGAAAGGAACAAGAAATGTATTATTGCCAATGGAACAGGTAATATATTATCATCAACATTCGAAGCGTTTTCGAAACCCGAAAAATTTGATAGAAGCATCGAGATCTTAGAAGAAATCTTATCAGCCGTGACAATGATGTTCCCTCTACAAGAAGAAGCCACTAAACACTTGAAATCAGAAACTTCATTACAATCATTGGTATGGTTTCTAAAAGGAGGAGATATATCAGGAAGAAGAAATTCAGTTTTGGTATTGAAAGAGATAATTTCATCATACCCAGAAAAAGTTAATGAATTAGGAGAAATCCAAGGAGCATTAGAGGGTCTTATTAAGCTCATAAAAGACCCAATTTGCTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGTCATAGCCTCAACTTCTTCTTCCAATCAAGAGAGATTCACAAAAGCACTTCTTCAAATGGGGTTGGTCTCTTTGCTACTCGAAACCCTCGTCGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGCGTTCGACGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCACGCTCTGACGATGCCTGTGATTGTGAAAAAGATACTTAGAGTATCGGATTTGGCGACCGAGTTATCGGTCTCCATTGTTTGGAAGCTTGTTAAGCATGAGAGTAGAGAAGATGAAGAAGAAGATGGAGGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGCAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGATAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACATTTTGATTGAAAAGTTAATTACTTAAATTCATGAGGATATAATGCAAATAATGTAAGTTGGTTGTGTGGGTGAGGATTTTGTGGCCTTTTCCTCTTTTTTTTTCTTCCCATTTTTATACGATTTCATTGTTGTACAAAGAAATTAAAGTAAAAAACAAATATAGGGATGTCCTTGTTAAACAACTATGT

Coding sequence (CDS)

ATGATGATTTCTTCTTGGAGACGGCGGAGAGCCACCCGCCGAATGGCAAGGGGTGGTGTCAGCGATGCGGTGGAGTTGACAATCCCGAGTCATTTTCGGTGCCCGATATCGCTCGACTTGATGAAAGATCCGGTGACTTTGTCGACGGGGATAACGTACGATCGAGCTAGCATTGAGACTTGGATTGAAGGAGGGAATTTCAGCTGCCCCTTCACCAACCAGCCATTGCAAAGTATTGATTCTATTCCTAATCATAACATAAGGAAGATGATCCAAGATTGGTGTGTTGAGAATAGAGCTTATGGGATTGAGAGGATTCCGACACCGAGGGTTCCGGCCAGTCCGGCGCAGGTTCGGGAAATTCTTGAGAAGATTTCCAGCGCAGCACGACGGGGGGATTGTGATGGATGCAAGTCCATGGTGGAGAAGATCAAGAAATTGGGGAAAGAAAGTGAAAGGAACAAGAAATGTATTATTGCCAATGGAACAGGTAATATATTATCATCAACATTCGAAGCGTTTTCGAAACCCGAAAAATTTGATAGAAGCATCGAGATCTTAGAAGAAATCTTATCAGCCGTGACAATGATGTTCCCTCTACAAGAAGAAGCCACTAAACACTTGAAATCAGAAACTTCATTACAATCATTGGTATGGTTTCTAAAAGGAGGAGATATATCAGGAAGAAGAAATTCAGTTTTGGTATTGAAAGAGATAATTTCATCATACCCAGAAAAAGTTAATGAATTAGGAGAAATCCAAGGAGCATTAGAGGGTCTTATTAAGCTCATAAAAGACCCAATTTGCTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGTCATAGCCTCAACTTCTTCTTCCAATCAAGAGAGATTCACAAAAGCACTTCTTCAAATGGGGTTGGTCTCTTTGCTACTCGAAACCCTCGTCGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGCGTTCGACGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCACGCTCTGACGATGCCTGTGATTGTGAAAAAGATACTTAGAGTATCGGATTTGGCGACCGAGTTATCGGTCTCCATTGTTTGGAAGCTTGTTAAGCATGAGAGTAGAGAAGATGAAGAAGAAGATGGAGGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGCAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGATAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACATTTTGA

Protein sequence

MMISSWRRRRATRRMARGGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDSLDFKDLKRTF

Homology

[Close]

BLAST of Lsi04G009910 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.7e-105
Identity = 210/423 (49.65%), Postives = 277/423 (65.48%), Query Frame = 0


Query: 25  ELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQPLQSIDSIPN 84
           E+TIP  F+CPIS+DLMKDPV +STGITYDR SIETWI  GN +CP TN  L + D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 85  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSMVEKI 144
           H IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+SSA RRGD + C  ++EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 145 KKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPLQEEA 204
           KKLG ESE+N+KC+  N  G +L   F+ FS  EK      +L EILS +T MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKL---TFMLNEILSLLTWMFPIGLEG 207

Query: 205 TKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLIKLI 264
              L S TS + +   LK  D S R+N+  ++KEI+S    +V+      G  E L+KLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 265 KDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETLVDAERSVCERAL 324
           +D + SSS K+SL   Y ++       +       L++GLVS+ +E +VDAE SVCE+AL
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVL-----QKPEIASEFLEIGLVSITVEMIVDAENSVCEKAL 327

Query: 325 GAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREDEEED 384
              D ICET+ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    E  
Sbjct: 328 AVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVE-- 387

Query: 385 GGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDSL-DFKDLK 444
                +A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK +K
Sbjct: 388 -----DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIK 435

Query: 445 RTF 447
           + F
Sbjct: 448 KPF 435

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BLAST of Lsi04G009910 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.9e-91
Identity = 194/432 (44.91%), Postives = 277/432 (64.12%), Query Frame = 0


Query: 21  SDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQPLQSID 80
           S+ VE+TIPS F+CPIS +LMKDPV +++GITYDR +IE W E G  +CP TN  L S++
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 81  SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSM 140
            IPNH IR+MIQ WC  +   GIERIPTPRVP +  QV EI E++S+A RRGD   C  M
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 141 VEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPL 200
           V K+ +LGKESERN+KC+  NG G +L   F+AFS  E  + S+ +LEE +S +T M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPI 205

Query: 201 QEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGL 260
             E    L + +S   LV  L+ GD    +N+  ++KE++      V+ L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 261 IKLI-KDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETLVDAERSV 320
           +K I +D  C +    SL   +H+I     +NQE  ++  L++ LV++ +E LVD+E SV
Sbjct: 266 MKSINRDSTCVN----SLISIHHMIL----TNQETVSR-FLELDLVNITVEMLVDSENSV 325

Query: 321 CERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESRE 380
           CE+AL   + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K     
Sbjct: 326 CEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK----- 385

Query: 381 DEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR-----DRLECI 440
               DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +     DR    
Sbjct: 386 --SGDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDR-SYS 431

Query: 441 DSLDFKDLKRTF 447
            S++FK +K+ F
Sbjct: 446 SSIEFKHVKKPF 431

[Close]

BLAST of Lsi04G009910 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 8.2e-55
Identity = 151/423 (35.70%), Postives = 223/423 (52.72%), Query Frame = 0


Query: 26  LTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIE-GGNFSCPFTNQPLQSIDSIPN 85
           + IP HFRCPISL+LM+DPVT+ TG TYDRASIE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 86  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSMVEKI 145
           H +R++IQ+WCV NR+ G+ERIPTP+ PA P  VR +L + S  A  G     +S    +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQAS--AITGTHVSVRSRAAAL 131

Query: 146 KKL---GKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPLQ 205
           ++L    ++S++N+  I A+    IL      FS+      S E++ E L+ + M+   +
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESLALLVMLPITE 191

Query: 206 EEATKHLKSETS-LQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEI---QGAL 265
                 + S+   ++ L   L    I  R N+  ++ EI+S+  +  +  G I   +   
Sbjct: 192 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALI-EIVSTGTKSADLKGSISNSESVF 251

Query: 266 EGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETL-VDAE 325
           EG++ L+++PI S         T   + S  S+     T      G   +L++ L  D +
Sbjct: 252 EGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAIT-----AGAPEILIDRLAADFD 311

Query: 326 RSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHE 385
           R   ERAL   + +C T +G      HALT+P++VK ILRVSD ATE +   +  L   E
Sbjct: 312 RCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAE 371

Query: 386 SREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDS 440
            R  EE  G         G   +LLL++Q  C+E  K+KA +LLKLL +   D     +S
Sbjct: 372 ERWREEAAG--------AGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANS 412

[Close]

BLAST of Lsi04G009910 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.5e-53
Identity = 138/404 (34.16%), Postives = 207/404 (51.24%), Query Frame = 0


Query: 26  LTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQPLQSIDSIPNH 85
           + IP HFRCPISLDLM DPVT+STG TYDR SI++WI  GN +CP T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 86  NIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISS-AARRGDCDGCKSMVEKI 145
            +R++IQ+WCV NR+ G+ERIPTP+ PA P  VR +L + S+            + + ++
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 146 KKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPLQEEA 205
           + L ++SE+N+  I  +    IL      F+  E    S E++ E L+ + ++   + E 
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTETEC 191

Query: 206 TKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNEL--GEIQGALEGLIK 265
                  + +  +   L    I  R N+  +++ +++       +L         EG++ 
Sbjct: 192 EAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVLD 251

Query: 266 LIKDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETL-VDAERSVCE 325
           L+K+PI   S + +L I    I +     Q R     +  G   +L++ L  D +R   E
Sbjct: 252 LLKNPI---SSRRALKIGIKAIFALCLVKQTR--HLAISAGAPGILIDRLAADFDRCDTE 311

Query: 326 RALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREDE 385
           R L   + +C   +G      HALT+P++VK ILRVSD ATE +   +  L   E R   
Sbjct: 312 RGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEER--- 371

Query: 386 EEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 426
                 + EA   G   +LLLL+Q  C+E  K KA  LLKLL +
Sbjct: 372 -----CRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRD 400

[Close]

BLAST of Lsi04G009910 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-50
Identity = 131/419 (31.26%), Postives = 224/419 (53.46%), Query Frame = 0


Query: 18  GGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWI-EGGNFSCPFTNQPL 77
           GG+ D  E+ IP  F CPISL++MKDPV +STGITYDR SIE W+  G   SCP T Q +
Sbjct: 3   GGIMDE-EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI 62

Query: 78  QSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREIL-EKISSAARRGDCD 137
              D  PNH +R++IQ WC  N +YG+ERIPTPR P   +++ +++ +  SS   +  C 
Sbjct: 63  TDADLTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKC- 122

Query: 138 GCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKF-DRSIEILEEILSAV 197
                +++++++  E+  NK+C+ A G    L++     S+     D ++ +L  + ++ 
Sbjct: 123 -----LKRLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSE 182

Query: 198 TMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQ 257
           T++    +    + K    ++SL   ++ G    R  + L+LK I+    + +  +    
Sbjct: 183 TVL----KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL-EVADPMQSMTLKP 242

Query: 258 GALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETLVD 317
                +++++ D I   + KA++    H++ +     + R     ++ G++S+++E L+D
Sbjct: 243 EVFTEVVQILDDRISQKATKAAM----HILVNICPWGRNR--HKAVEAGVISVIIELLMD 302

Query: 318 ----AERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVW 377
               +ER   E A+   D +C+  +GR E   H   + V+ KKILRVS  A++ +V ++ 
Sbjct: 303 ESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLL 362

Query: 378 KLVKHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRD 430
            + +  +         +  E LQ+G   KL L+LQV C   TKEKA ELLKL   + +D
Sbjct: 363 SVGRFCATP------ALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKD 397

[Close]

BLAST of Lsi04G009910 vs. ExPASy TrEMBL
Match: A0A0A0KMI8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE=4 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 5.6e-232
Identity = 414/446 (92.83%), Postives = 432/446 (96.86%), Query Frame = 0


Query: 2   MISSWRRRRATRRMARGGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETW 61
           MISSWRRRRATRRMARGG+S  +ELTIP+HFRCPISLDLMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRMARGGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 62  IEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 121
           IEGGNF+CPFTNQPLQ+IDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 120

Query: 122 LEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFD 181
           LEK+++AARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGN LSS FEAFS PE F+
Sbjct: 121 LEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETFE 180

Query: 182 RSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 241
           + IEILEEILSA+T+ FPLQEE+ KHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS
Sbjct: 181 KRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 240

Query: 242 SYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIAST-SSSNQERFTKALL 301
           SYPEKV+ELGEIQGALEGLIKLIKDPICSSS+KASLFITYHVIAST SSSN+ERFTK LL
Sbjct: 241 SYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTKELL 300

Query: 302 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 361
           QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 362 ATELSVSIVWKLVKHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 421
           ATELSV IVWKLVKHES+  EEEDGG+KVEALQVGAFQKLLLLLQVGCSEWTKEKATELL
Sbjct: 361 ATELSVCIVWKLVKHESK--EEEDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 420

Query: 422 KLLNNLHRDRLECIDSLDFKDLKRTF 447
           KLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 KLLNNLHRDRLECIDSLDFKDLKRTF 444

[Close]

BLAST of Lsi04G009910 vs. ExPASy TrEMBL
Match: A0A1S3CPH4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.6e-226
Identity = 407/446 (91.26%), Postives = 427/446 (95.74%), Query Frame = 0


Query: 2   MISSWRRRRATRRMARGGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETW 61
           MISSWRRRRAT RM RG + D VELTIP+HFRCPISLDLMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATPRMPRGSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 62  IEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 121
           IEGGNF+CPFTNQPLQ+IDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+I
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDI 120

Query: 122 LEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFD 181
           LEK+++AARRGD +GCKSMVEKIK LGKESERNKKCIIANGTGN  SS FEAFS PE F+
Sbjct: 121 LEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFE 180

Query: 182 RSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 241
           + IEILEEILSA+TM+FPLQEE+ KHLKSETSLQSL WFLKGGDISGRRNSVLVLKEIIS
Sbjct: 181 KRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIIS 240

Query: 242 SYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIAST-SSSNQERFTKALL 301
           SYPEKV+ELG IQGALEGLIKLIKDPICSSSKKASLFITYHVIAST SSSNQ RFTKALL
Sbjct: 241 SYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALL 300

Query: 302 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 361
           QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 362 ATELSVSIVWKLVKHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 421
           ATELSVSIVWKLVK+ES+E E+EDGG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELL
Sbjct: 361 ATELSVSIVWKLVKNESKE-EKEDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 420

Query: 422 KLLNNLHRDRLECIDSLDFKDLKRTF 447
           KLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 KLLNNLHRDRLECIDSLDFKDLKRTF 445

[Close]

BLAST of Lsi04G009910 vs. ExPASy TrEMBL
Match: A0A5D3BPA4 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold701G00820 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.9e-220
Identity = 395/433 (91.22%), Postives = 415/433 (95.84%), Query Frame = 0


Query: 15  MARGGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQ 74
           M RG + D VELTIP+HFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNF+CPFTNQ
Sbjct: 1   MPRGSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFTCPFTNQ 60

Query: 75  PLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDC 134
           PLQ+IDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+ILEK+++AARRGD 
Sbjct: 61  PLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDILEKMTAAARRGDF 120

Query: 135 DGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAV 194
           +GCKSMVEKIK LGKESERNKKCIIANGTGN  SS FEAFS PE F++ IEILEEILSA+
Sbjct: 121 EGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFEKRIEILEEILSAM 180

Query: 195 TMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQ 254
           TM+FPLQEE+ KHLKSETSLQSL WFLKGGDISGRRNSVLVLKEIISSYPEKV+ELG IQ
Sbjct: 181 TMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIISSYPEKVDELGVIQ 240

Query: 255 GALEGLIKLIKDPICSSSKKASLFITYHVIAST-SSSNQERFTKALLQMGLVSLLLETLV 314
           GALEGLIKLIKDPICSSSKKASLFITYHVIAST SSSNQ RFTKALLQMGLVSLLLETLV
Sbjct: 241 GALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALLQMGLVSLLLETLV 300

Query: 315 DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV 374
           DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV
Sbjct: 301 DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV 360

Query: 375 KHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 434
           K+ES+E E+EDGG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC
Sbjct: 361 KNESKE-EKEDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 420

Query: 435 IDSLDFKDLKRTF 447
           IDSLDFKDLKRTF
Sbjct: 421 IDSLDFKDLKRTF 432

[Close]

BLAST of Lsi04G009910 vs. ExPASy TrEMBL
Match: A0A6J1K7Y6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 PE=4 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 1.1e-214
Identity = 386/448 (86.16%), Postives = 417/448 (93.08%), Query Frame = 0


Query: 1   MMISSWRRRRATRRMARGGVSDAV-ELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIE 60
           MM+ SWRRRRATRRMARGG   ++ ELTIPSHF CPISLDLMKDPVT+STGITYDRA+IE
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIE 60

Query: 61  TWIEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 120
           TWIEGGNFSCPFTNQPL +IDSIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP QVR
Sbjct: 61  TWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVR 120

Query: 121 EILEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEK 180
           EIL+KI++AA R DC+ CKSMVEKIK L +ESERNKKCIIANGTGN+LSS FEAFSKPE+
Sbjct: 121 EILDKIAAAAGREDCEECKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPER 180

Query: 181 FDRSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEI 240
           F++ +EILEEILS + M+FPLQEEA KHLKSE S+Q+LVWF++G D S RR+S+LVLKEI
Sbjct: 181 FEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEI 240

Query: 241 ISSYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTKAL 300
           ISSYPEKV+ELGEIQG LEG++KLIK+PICSSSKKASLFITYH IA  SSSNQE FTKA 
Sbjct: 241 ISSYPEKVSELGEIQGVLEGVMKLIKEPICSSSKKASLFITYHAIA--SSSNQEGFTKAF 300

Query: 301 LQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360
           LQMGLVSLLLETL+DAERSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRVSD
Sbjct: 301 LQMGLVSLLLETLIDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 361 LATELSVSIVWKLVKHESREDE-EEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420
           LATELSVSIVWKLVKHESREDE EEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE
Sbjct: 361 LATELSVSIVWKLVKHESREDEDEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420

Query: 421 LLKLLNNLHRDRLECIDSLDFKDLKRTF 447
           LLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 446

[Close]

BLAST of Lsi04G009910 vs. ExPASy TrEMBL
Match: A0A6J1FPJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642 PE=4 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.0e-214
Identity = 385/450 (85.56%), Postives = 414/450 (92.00%), Query Frame = 0


Query: 1   MMISSWRRRRATRRMAR---GGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRAS 60
           MM+ SWRRRRATRRMAR   GG     ELTIPSHF CPISLDLMKDPVT+STGITYDRA+
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60

Query: 61  IETWIEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQ 120
           IETWIEGGNFSCPFTNQPL +IDSIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP Q
Sbjct: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120

Query: 121 VREILEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKP 180
           VREIL+KI++A RR DC+GCKS+VEKIK L +ESERNKKCIIANGTGN+LSS FEAFSKP
Sbjct: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180

Query: 181 EKFDRSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLK 240
           E+F++ +EILEEILS + M+FPLQEEA KHLKSE S+Q+LVWF++G D S RR+S+LVLK
Sbjct: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240

Query: 241 EIISSYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTK 300
           EIISSYPEKV+ELGEIQG LEGL+KLIK+PICSSSKKASLFITYH IA  SSSNQE FTK
Sbjct: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIA--SSSNQEGFTK 300

Query: 301 ALLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRV 360
           A LQMGLV LLLETLVDAERSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRV
Sbjct: 301 AFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRV 360

Query: 361 SDLATELSVSIVWKLVKHESREDEE-EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 420
           SDL TELSVSIVWKLVKHESRE+EE EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 SDLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 421 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 447
           TELLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRERLECIDSLDFKDLKRTF 448

[Close]

BLAST of Lsi04G009910 vs. NCBI nr
Match: XP_038892848.1 (U-box domain-containing protein 21-like [Benincasa hispida])

HSP 1 Score: 828.6 bits (2139), Expect = 2.6e-236
Identity = 424/448 (94.64%), Postives = 436/448 (97.32%), Query Frame = 0


Query: 1   MMISSWRRRRATRRMARGGVS-DAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIE 60
           MMISSWRRRRA RRMAR GVS D VELTIPSHFRCPISL+LMKDPVTLSTGITYDRASIE
Sbjct: 1   MMISSWRRRRAARRMARVGVSGDTVELTIPSHFRCPISLELMKDPVTLSTGITYDRASIE 60

Query: 61  TWIEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 120
           TW+EGGNF+CPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR
Sbjct: 61  TWMEGGNFTCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 120

Query: 121 EILEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEK 180
           EILEK+++A RRGDCDGCKS+VEKIKKLGKESERNKKCII NGTGN LSSTFEAFS PEK
Sbjct: 121 EILEKMATATRRGDCDGCKSLVEKIKKLGKESERNKKCIINNGTGNTLSSTFEAFSNPEK 180

Query: 181 FDRSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEI 240
           F++SIEILEEILSA+TMMFPLQEEA KHLKSETSLQSLVWF+KGGDISGRRNSVLVLKEI
Sbjct: 181 FEKSIEILEEILSAMTMMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEI 240

Query: 241 ISSYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTKAL 300
           ISSYPEKVNELGEIQGALEGL KLIKDPICSSSKKASLF+TYHVIASTSS NQERFTKAL
Sbjct: 241 ISSYPEKVNELGEIQGALEGLFKLIKDPICSSSKKASLFMTYHVIASTSSPNQERFTKAL 300

Query: 301 LQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360
           LQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSD
Sbjct: 301 LQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 361 LATELSVSIVWKLVKHESRED-EEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420
           LATELSVSIVWKLVKHESRED EEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE
Sbjct: 361 LATELSVSIVWKLVKHESREDEEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420

Query: 421 LLKLLNNLHRDRLECIDSLDFKDLKRTF 447
           LLKLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 LLKLLNNLHRDRLECIDSLDFKDLKRTF 448

[Close]

BLAST of Lsi04G009910 vs. NCBI nr
Match: XP_004142305.1 (U-box domain-containing protein 21 [Cucumis sativus] >KGN49607.1 hypothetical protein Csa_018466 [Cucumis sativus])

HSP 1 Score: 813.1 bits (2099), Expect = 1.2e-231
Identity = 414/446 (92.83%), Postives = 432/446 (96.86%), Query Frame = 0


Query: 2   MISSWRRRRATRRMARGGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETW 61
           MISSWRRRRATRRMARGG+S  +ELTIP+HFRCPISLDLMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRMARGGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 62  IEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 121
           IEGGNF+CPFTNQPLQ+IDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 120

Query: 122 LEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFD 181
           LEK+++AARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGN LSS FEAFS PE F+
Sbjct: 121 LEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETFE 180

Query: 182 RSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 241
           + IEILEEILSA+T+ FPLQEE+ KHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS
Sbjct: 181 KRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 240

Query: 242 SYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIAST-SSSNQERFTKALL 301
           SYPEKV+ELGEIQGALEGLIKLIKDPICSSS+KASLFITYHVIAST SSSN+ERFTK LL
Sbjct: 241 SYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTKELL 300

Query: 302 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 361
           QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 362 ATELSVSIVWKLVKHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 421
           ATELSV IVWKLVKHES+  EEEDGG+KVEALQVGAFQKLLLLLQVGCSEWTKEKATELL
Sbjct: 361 ATELSVCIVWKLVKHESK--EEEDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 420

Query: 422 KLLNNLHRDRLECIDSLDFKDLKRTF 447
           KLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 KLLNNLHRDRLECIDSLDFKDLKRTF 444

[Close]

BLAST of Lsi04G009910 vs. NCBI nr
Match: XP_008465536.1 (PREDICTED: U-box domain-containing protein 21-like [Cucumis melo])

HSP 1 Score: 795.0 bits (2052), Expect = 3.2e-226
Identity = 407/446 (91.26%), Postives = 427/446 (95.74%), Query Frame = 0


Query: 2   MISSWRRRRATRRMARGGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETW 61
           MISSWRRRRAT RM RG + D VELTIP+HFRCPISLDLMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATPRMPRGSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 62  IEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 121
           IEGGNF+CPFTNQPLQ+IDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+I
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDI 120

Query: 122 LEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFD 181
           LEK+++AARRGD +GCKSMVEKIK LGKESERNKKCIIANGTGN  SS FEAFS PE F+
Sbjct: 121 LEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFE 180

Query: 182 RSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 241
           + IEILEEILSA+TM+FPLQEE+ KHLKSETSLQSL WFLKGGDISGRRNSVLVLKEIIS
Sbjct: 181 KRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIIS 240

Query: 242 SYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIAST-SSSNQERFTKALL 301
           SYPEKV+ELG IQGALEGLIKLIKDPICSSSKKASLFITYHVIAST SSSNQ RFTKALL
Sbjct: 241 SYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALL 300

Query: 302 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 361
           QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 362 ATELSVSIVWKLVKHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 421
           ATELSVSIVWKLVK+ES+E E+EDGG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELL
Sbjct: 361 ATELSVSIVWKLVKNESKE-EKEDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELL 420

Query: 422 KLLNNLHRDRLECIDSLDFKDLKRTF 447
           KLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 KLLNNLHRDRLECIDSLDFKDLKRTF 445

[Close]

BLAST of Lsi04G009910 vs. NCBI nr
Match: KAA0056826.1 (U-box domain-containing protein 21-like [Cucumis melo var. makuwa] >TYJ99948.1 U-box domain-containing protein 21-like [Cucumis melo var. makuwa])

HSP 1 Score: 773.5 bits (1996), Expect = 1.0e-219
Identity = 395/433 (91.22%), Postives = 415/433 (95.84%), Query Frame = 0


Query: 15  MARGGVSDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQ 74
           M RG + D VELTIP+HFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNF+CPFTNQ
Sbjct: 1   MPRGSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFTCPFTNQ 60

Query: 75  PLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDC 134
           PLQ+IDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+ILEK+++AARRGD 
Sbjct: 61  PLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDILEKMTAAARRGDF 120

Query: 135 DGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAV 194
           +GCKSMVEKIK LGKESERNKKCIIANGTGN  SS FEAFS PE F++ IEILEEILSA+
Sbjct: 121 EGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFEKRIEILEEILSAM 180

Query: 195 TMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQ 254
           TM+FPLQEE+ KHLKSETSLQSL WFLKGGDISGRRNSVLVLKEIISSYPEKV+ELG IQ
Sbjct: 181 TMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIISSYPEKVDELGVIQ 240

Query: 255 GALEGLIKLIKDPICSSSKKASLFITYHVIAST-SSSNQERFTKALLQMGLVSLLLETLV 314
           GALEGLIKLIKDPICSSSKKASLFITYHVIAST SSSNQ RFTKALLQMGLVSLLLETLV
Sbjct: 241 GALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALLQMGLVSLLLETLV 300

Query: 315 DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV 374
           DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV
Sbjct: 301 DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV 360

Query: 375 KHESREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 434
           K+ES+E E+EDGG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC
Sbjct: 361 KNESKE-EKEDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 420

Query: 435 IDSLDFKDLKRTF 447
           IDSLDFKDLKRTF
Sbjct: 421 IDSLDFKDLKRTF 432

[Close]

BLAST of Lsi04G009910 vs. NCBI nr
Match: XP_022996865.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 755.7 bits (1950), Expect = 2.2e-214
Identity = 386/448 (86.16%), Postives = 417/448 (93.08%), Query Frame = 0


Query: 1   MMISSWRRRRATRRMARGGVSDAV-ELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIE 60
           MM+ SWRRRRATRRMARGG   ++ ELTIPSHF CPISLDLMKDPVT+STGITYDRA+IE
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIE 60

Query: 61  TWIEGGNFSCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 120
           TWIEGGNFSCPFTNQPL +IDSIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP QVR
Sbjct: 61  TWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVR 120

Query: 121 EILEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEK 180
           EIL+KI++AA R DC+ CKSMVEKIK L +ESERNKKCIIANGTGN+LSS FEAFSKPE+
Sbjct: 121 EILDKIAAAAGREDCEECKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPER 180

Query: 181 FDRSIEILEEILSAVTMMFPLQEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEI 240
           F++ +EILEEILS + M+FPLQEEA KHLKSE S+Q+LVWF++G D S RR+S+LVLKEI
Sbjct: 181 FEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEI 240

Query: 241 ISSYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTKAL 300
           ISSYPEKV+ELGEIQG LEG++KLIK+PICSSSKKASLFITYH IA  SSSNQE FTKA 
Sbjct: 241 ISSYPEKVSELGEIQGVLEGVMKLIKEPICSSSKKASLFITYHAIA--SSSNQEGFTKAF 300

Query: 301 LQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360
           LQMGLVSLLLETL+DAERSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRVSD
Sbjct: 301 LQMGLVSLLLETLIDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 361 LATELSVSIVWKLVKHESREDE-EEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420
           LATELSVSIVWKLVKHESREDE EEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE
Sbjct: 361 LATELSVSIVWKLVKHESREDEDEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420

Query: 421 LLKLLNNLHRDRLECIDSLDFKDLKRTF 447
           LLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 446

[Close]

BLAST of Lsi04G009910 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.2e-106
Identity = 210/423 (49.65%), Postives = 277/423 (65.48%), Query Frame = 0


Query: 25  ELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQPLQSIDSIPN 84
           E+TIP  F+CPIS+DLMKDPV +STGITYDR SIETWI  GN +CP TN  L + D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 85  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSMVEKI 144
           H IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+SSA RRGD + C  ++EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 145 KKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPLQEEA 204
           KKLG ESE+N+KC+  N  G +L   F+ FS  EK      +L EILS +T MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKL---TFMLNEILSLLTWMFPIGLEG 207

Query: 205 TKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLIKLI 264
              L S TS + +   LK  D S R+N+  ++KEI+S    +V+      G  E L+KLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 265 KDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETLVDAERSVCERAL 324
           +D + SSS K+SL   Y ++       +       L++GLVS+ +E +VDAE SVCE+AL
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVL-----QKPEIASEFLEIGLVSITVEMIVDAENSVCEKAL 327

Query: 325 GAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREDEEED 384
              D ICET+ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    E  
Sbjct: 328 AVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVE-- 387

Query: 385 GGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDSL-DFKDLK 444
                +A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK +K
Sbjct: 388 -----DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIK 435

Query: 445 RTF 447
           + F
Sbjct: 448 KPF 435

[Close]

BLAST of Lsi04G009910 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 337.8 bits (865), Expect = 1.3e-92
Identity = 194/432 (44.91%), Postives = 277/432 (64.12%), Query Frame = 0


Query: 21  SDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQPLQSID 80
           S+ VE+TIPS F+CPIS +LMKDPV +++GITYDR +IE W E G  +CP TN  L S++
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 81  SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSM 140
            IPNH IR+MIQ WC  +   GIERIPTPRVP +  QV EI E++S+A RRGD   C  M
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 141 VEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPL 200
           V K+ +LGKESERN+KC+  NG G +L   F+AFS  E  + S+ +LEE +S +T M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPI 205

Query: 201 QEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGL 260
             E    L + +S   LV  L+ GD    +N+  ++KE++      V+ L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 261 IKLI-KDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETLVDAERSV 320
           +K I +D  C +    SL   +H+I     +NQE  ++  L++ LV++ +E LVD+E SV
Sbjct: 266 MKSINRDSTCVN----SLISIHHMIL----TNQETVSR-FLELDLVNITVEMLVDSENSV 325

Query: 321 CERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESRE 380
           CE+AL   + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K     
Sbjct: 326 CEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK----- 385

Query: 381 DEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR-----DRLECI 440
               DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +     DR    
Sbjct: 386 --SGDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDR-SYS 431

Query: 441 DSLDFKDLKRTF 447
            S++FK +K+ F
Sbjct: 446 SSIEFKHVKKPF 431

[Close]

BLAST of Lsi04G009910 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-83
Identity = 185/432 (42.82%), Postives = 264/432 (61.11%), Query Frame = 0


Query: 21  SDAVELTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFSCPFTNQPLQSID 80
           S+ VE+TIPS F+CPIS +LMKDP                W E G  +CP TN  L S++
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDP----------------WFESGYQTCPVTNTVLTSLE 85

Query: 81  SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSM 140
            IPNH IR+MIQ WC  +   GIERIPTPRVP +  QV EI E++S+A RRGD   C  M
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 141 VEKIKKLGKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPL 200
           V K+ +LGKESERN+KC+  NG G +L   F+AFS  E  + S+ +LEE +S +T M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPI 205

Query: 201 QEEATKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGL 260
             E    L + +S   LV  L+ GD    +N+  ++KE++      V+ L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 261 IKLI-KDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETLVDAERSV 320
           +K I +D  C +    SL   +H+I     +NQE  ++  L++ LV++ +E LVD+E SV
Sbjct: 266 MKSINRDSTCVN----SLISIHHMIL----TNQETVSR-FLELDLVNITVEMLVDSENSV 325

Query: 321 CERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESRE 380
           CE+AL   + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K     
Sbjct: 326 CEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK----- 385

Query: 381 DEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR-----DRLECI 440
               DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +     DR    
Sbjct: 386 --SGDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDR-SYS 415

Query: 441 DSLDFKDLKRTF 447
            S++FK +K+ F
Sbjct: 446 SSIEFKHVKKPF 415

[Close]

BLAST of Lsi04G009910 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.3e-68
Identity = 152/349 (43.55%), Postives = 226/349 (64.76%), Query Frame = 0


Query: 103 IERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANG 162
           IERI +PRV  +P    EI  ++ +AA R +   C  +V KIK LG+  + NKKC++ NG
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 163 TGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPL-QEEATKHLKSETSLQSLVWFL 222
           +   LSS FE F+     D  + +LEEIL  ++   PL + E    + S  SL  LV FL
Sbjct: 100 SVLALSSCFERFAAAR--DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFL 159

Query: 223 KGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLIKLIKDPICSSSKKASLFITY 282
            G D   R+N+   ++E+I+     V  L +++GA EGL+K+I+D + +SS KASL + Y
Sbjct: 160 NGKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIY 219

Query: 283 HVIASTSSSNQERFTKALLQMGLVSLLLETLV-DAERSVCERALGAFDGICETKQGREEA 342
             I     S+ ++ T+  +++GLV L+ E +V +AE+SVCER+L   +  C+ +QG+E+ 
Sbjct: 220 RAI-----SSNDKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDV 279

Query: 343 YAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREDEEEDGGVKVEALQVGAFQKL 402
             +AL +P++VKKILRVSDLAT+ SVSI+WKL K  +++D E D  + VEALQVGAF+KL
Sbjct: 280 LRNALIVPLLVKKILRVSDLATQCSVSILWKLWK--NKKDGECDDRLLVEALQVGAFEKL 339

Query: 403 LLLLQVGCSEWTKEKATELLKLLNNLHR--DRLECID-SLDFKDLKRTF 447
           L+LLQVGC + TKEKA+ELL+ LN      ++  C+D S+  K++K++F
Sbjct: 340 LVLLQVGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

[Close]

BLAST of Lsi04G009910 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 216.1 bits (549), Expect = 5.8e-56
Identity = 151/423 (35.70%), Postives = 223/423 (52.72%), Query Frame = 0


Query: 26  LTIPSHFRCPISLDLMKDPVTLSTGITYDRASIETWIE-GGNFSCPFTNQPLQSIDSIPN 85
           + IP HFRCPISL+LM+DPVT+ TG TYDRASIE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 86  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKISSAARRGDCDGCKSMVEKI 145
           H +R++IQ+WCV NR+ G+ERIPTP+ PA P  VR +L + S  A  G     +S    +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQAS--AITGTHVSVRSRAAAL 131

Query: 146 KKL---GKESERNKKCIIANGTGNILSSTFEAFSKPEKFDRSIEILEEILSAVTMMFPLQ 205
           ++L    ++S++N+  I A+    IL      FS+      S E++ E L+ + M+   +
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESLALLVMLPITE 191

Query: 206 EEATKHLKSETS-LQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVNELGEI---QGAL 265
                 + S+   ++ L   L    I  R N+  ++ EI+S+  +  +  G I   +   
Sbjct: 192 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALI-EIVSTGTKSADLKGSISNSESVF 251

Query: 266 EGLIKLIKDPICSSSKKASLFITYHVIASTSSSNQERFTKALLQMGLVSLLLETL-VDAE 325
           EG++ L+++PI S         T   + S  S+     T      G   +L++ L  D +
Sbjct: 252 EGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAIT-----AGAPEILIDRLAADFD 311

Query: 326 RSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHE 385
           R   ERAL   + +C T +G      HALT+P++VK ILRVSD ATE +   +  L   E
Sbjct: 312 RCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAE 371

Query: 386 SREDEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDS 440
            R  EE  G         G   +LLL++Q  C+E  K+KA +LLKLL +   D     +S
Sbjct: 372 ERWREEAAG--------AGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANS 412

The following BLAST results are available for this feature:

BLAST of Lsi04G009910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5PNY6	1.7e-105	49.65	U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2...	[more]
Q9C8D1	1.9e-91	44.91	U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1...	[more]
Q9LT79	8.2e-55	35.70	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	4.5e-53	34.16	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]
Q84TG3	2.1e-50	31.26	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]

BLAST of Lsi04G009910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMI8	5.6e-232	92.83	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE...	[more]
A0A1S3CPH4	1.6e-226	91.26	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 ...	[more]
A0A5D3BPA4	4.9e-220	91.22	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1K7Y6	1.1e-214	86.16	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 P...	[more]
A0A6J1FPJ6	4.0e-214	85.56	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642...	[more]

BLAST of Lsi04G009910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892848.1	2.6e-236	94.64	U-box domain-containing protein 21-like [Benincasa hispida]	[more]
XP_004142305.1	1.2e-231	92.83	U-box domain-containing protein 21 [Cucumis sativus] >KGN49607.1 hypothetical pr...	[more]
XP_008465536.1	3.2e-226	91.26	PREDICTED: U-box domain-containing protein 21-like [Cucumis melo]	[more]
KAA0056826.1	1.0e-219	91.22	U-box domain-containing protein 21-like [Cucumis melo var. makuwa] >TYJ99948.1 U...	[more]
XP_022996865.1	2.2e-214	86.16	U-box domain-containing protein 21-like [Cucurbita maxima]	[more]

BLAST of Lsi04G009910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37490.1	1.2e-106	49.65	ARM repeat superfamily protein	[more]
AT1G66160.1	1.3e-92	44.91	CYS, MET, PRO, and GLY protein 1	[more]
AT1G66160.2	1.1e-83	42.82	CYS, MET, PRO, and GLY protein 1	[more]
AT3G02840.1	1.3e-68	43.55	ARM repeat superfamily protein	[more]
AT3G19380.1	5.8e-56	35.70	plant U-box 25	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 31..94 e-value: 9.0E-29 score: 111.6
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 28..99 e-value: 2.6E-20 score: 72.4
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 27..101 score: 40.41777
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 3..104 e-value: 2.2E-28 score: 100.1
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 115..426 e-value: 5.4E-16 score: 60.5
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 10..446
None	No IPR available	PANTHER	PTHR22849:SF139	ARM REPEAT SUPERFAMILY PROTEIN	coord: 10..446
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 32..74 e-value: 7.26041E-24 score: 91.5262
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 22..104
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 121..425

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G009910.1	Lsi04G009910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity