Homology
BLAST of Lsi04G009190 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 1068.9 bits (2763), Expect = 2.5e-311
Identity = 543/732 (74.18%), Postives = 605/732 (82.65%), Query Frame = 0
Query: 7 MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSF---- 66
MSSSLIFKL F ++FFS LAS LDS DD K IY+VYMGRKL +D DSAH H +
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60
Query: 67 ---PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLG 126
FAPESV++TYKRSFNGFAVKLT+EEAEKIA MEGVVSVF N+MN+LHTTRSWDFLG
Sbjct: 61 VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
Query: 127 FPKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKII 186
FP VPR QVE+N++VGVLD+GIWPESPSFDD+ F P P KWKG+C+ + NF CNRKII
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
Query: 187 GARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARI 246
GAR+Y IGR + GDV+ PRDTNGHGTHTASTAAGGL+SQA+ YGLGLGT RGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
Query: 247 AVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGI 306
A YK+CWNDGCSD D+LAA+DDAIADGVDIISLSVG R YF+D+IAIGSFHA + GI
Sbjct: 241 AAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300
Query: 307 LTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQ 366
LTSNSAGN GP FTT SLSPWLLSVAASTMDRKF+T+VQIGN SF+GVSINTFD N
Sbjct: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQY 360
Query: 367 YPLVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFF-NFGGAAGVLT 426
YPLV+G D+ T F STSR C + SVNP L+KGKI++C+ +FF + GAAGVL
Sbjct: 361 YPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420
Query: 427 VSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSR 486
S+TRD A S PLPSS LDPND YI+S RSP ATIFKS T+ N SAP + SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480
Query: 487 GPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYV 546
GPN TKD++KPD+S PGVEILAAWP VAPVGG R TL+NIISGTSMSCPHITGIA YV
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYV 540
Query: 547 KTFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYV 606
KT+NPTWSPAAIKSALMTTASPMNA+F+P+AEFAYGSGHVNPLKAVRPGLVYDANESDYV
Sbjct: 541 KTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYV 600
Query: 607 KFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSV 666
KFLCGQGY T VR IT D SACTS N GRVWDLNYPSFGL VS S+ F+QYF RTLTSV
Sbjct: 601 KFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSV 660
Query: 667 ASQASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGV 726
A QASTYRA IS P GL+I VNPNVLSFNG+GDRKSF LTVRG+++ ++VSASLVWSDGV
Sbjct: 661 APQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGV 720
Query: 727 HTVRSPITITSL 730
H VRSPITITSL
Sbjct: 721 HYVRSPITITSL 730
BLAST of Lsi04G009190 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 632.9 bits (1631), Expect = 4.6e-180
Identity = 349/721 (48.40%), Postives = 466/721 (64.63%), Query Frame = 0
Query: 25 LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 84
LL+S D+D ++Y+VYMG + D H S ES +V +YKRSF
Sbjct: 18 LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77
Query: 85 NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 144
NGFA +LT+ E IA++EGVVSVFPNK+ QLHTT SWDF+G KN R +E++ I
Sbjct: 78 NGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137
Query: 145 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 204
+GV+D+GIWPES SF DK FGP P KWKG C NFTCN K+IGAR Y
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197
Query: 205 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 264
+ RDT+GHGTHTASTAAG + SF+G+G GTVRGGVP++RIA YK+C + GCS L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257
Query: 265 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 324
L++FDDAIADGVD+I++S+GF+ + D IAIG+FHA GILT +SAGNSGPK T
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317
Query: 325 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 384
+ ++PW+ +VAAST +R FITKV +GN + G S+N FDM G +YPLV G ++
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377
Query: 385 NSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSDTRDTAFSNPLPSS 444
T+ C +N VKGKIL+C Y GA ++ S D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGY-KIAKSVGAIAIIDKSPRPDVAFTHHLPAS 437
Query: 445 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 504
L D ++ YI S SP A + K+ T+ N ++P +ASFSSRGPN I D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497
Query: 505 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 564
PGVEILAA+ P P R+ Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557
Query: 565 LMTTASPMNAKFD--PEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 624
+MTTA P+ AK EFAYG+GHV+P+ A+ PGLVY+ +++D++ FLCG YT+ +
Sbjct: 558 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 617
Query: 625 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 684
+II+ D C+ KN +LNYPS +S + F F RTLT+V + STY++ +
Sbjct: 618 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 677
Query: 685 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 727
+G LSIKV P+VL F + +++SF +TV G+ V SA+L+WSDG H VRSPI
Sbjct: 678 AGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIV 728
BLAST of Lsi04G009190 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 627.1 bits (1616), Expect = 2.5e-178
Identity = 342/719 (47.57%), Postives = 475/719 (66.06%), Query Frame = 0
Query: 32 SADDDKK---IYVVYMGRKLKDDRDSAHSHQSSF-------PFAPESVVYTYKRSFNGFA 91
SA+D ++ +Y+VYMG L + + S SH S A +V +YKRSFNGFA
Sbjct: 22 SANDYRQASSVYIVYMG-TLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFA 81
Query: 92 VKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPRVKQVENNVIVGVLDS 151
L++ E++K+ +M+ VVSVFP+K ++L TTRSWDF+GF + R E++VIVGV+DS
Sbjct: 82 ANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDS 141
Query: 152 GIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDVDSPRDT 211
GIWPES SFDD+ FGP P KWKGSC+ + F CN K+IGAR Y + DS RD
Sbjct: 142 GIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY-------NKFADSARDE 201
Query: 212 NGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDLLAAFDD 271
GHGTHTASTAAG + ASFYGL GT RGGVPSARIA YK+C+N C+D+D+LAAFDD
Sbjct: 202 EGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDD 261
Query: 272 AIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTTTSLSPW 331
AIADGVD+IS+S+ + S+AIGSFHA GI+T+ SAGN+GP + ++SPW
Sbjct: 262 AIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPW 321
Query: 332 LLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFHNSTSRS 391
+++VAAS DR+FI +V +GN + G+S+NTF++NG ++P+V G +V + + + +
Sbjct: 322 MITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGY 381
Query: 392 CRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSD-TRDTAFSNPLPSSTLDPN 451
C + V+ +LVKGKI++C + L Y + + GA GV+ + D+AF P P+S+L
Sbjct: 382 CSSGCVDSELVKGKIVLCDDFLGYREAY-LAGAIGVIVQNTLLPDSAFVVPFPASSLGFE 441
Query: 452 DADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSAPGVEI 511
D +I YI SA P A I ++ + + AP++ SFSSRGP+ + ++L+KPD+SAPG+EI
Sbjct: 442 DYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEI 501
Query: 512 LAAWPPVAPVGG-----SHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSAL 571
LAA+ PVA R Y+++SGTSM+CPH+ G+A YVK+F+P WSP+AIKSA+
Sbjct: 502 LAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAI 561
Query: 572 MTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNIVRII 631
MTTA+PMN K +PE EFAYGSG +NP KA PGLVY+ DY+K LC +G+ + +
Sbjct: 562 MTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTT 621
Query: 632 TNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYRAT-ISTPN 691
+ N C+ + V DLNYP+ FVS F+ F RT+T+V STY+A+ +
Sbjct: 622 SGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQP 681
Query: 692 GLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRK--YIVSASLVWSDGVHTVRSPITITSL 730
L I + P +L F + ++KSF +T+ G K VS+S+VWSDG H+VRSPI S+
Sbjct: 682 ELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727
BLAST of Lsi04G009190 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 622.1 bits (1603), Expect = 8.1e-177
Identity = 343/748 (45.86%), Postives = 484/748 (64.71%), Query Frame = 0
Query: 7 MSSSLIFKLVFLNIFFSTLLASSLDSAD-DDKKIYVVYMGR-KLKDDRDSAHSHQSSF-- 66
M+ F +F ++ +L + S D D D+++Y+VY+G +++ H S
Sbjct: 1 MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60
Query: 67 ----PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFL 126
+V +YK+SFNGFA +LT+ E +++A ME VVSVFP++ +L TT SW+F+
Sbjct: 61 ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120
Query: 127 GFPKNV--PRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNR 186
G + + R + +E++ I+GV+DSGI+PES SF D+ FGP P KWKG+C NFTCN
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180
Query: 187 KIIGARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPS 246
K+IGAR Y +S + + RD +GHGTHTAS AAG ++ ++FYGLG GT RGGVP+
Sbjct: 181 KVIGARDY-TAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPA 240
Query: 247 ARIAVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQ 306
ARIAVYK+C N+GC +++AFDDAIADGVD+IS+S+ + P+ D IAIG+FHA
Sbjct: 241 ARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMA 300
Query: 307 NGILTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDM 366
G+LT N+AGN+GPK+ T TS +PW+ SVAAS +R F+ KV +G+ G S+NT+DM
Sbjct: 301 VGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM 360
Query: 367 NG-QYPLVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAG 426
NG YPLV G ++ +R C ++ KLVKGKI++C + GA G
Sbjct: 361 NGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL-GAVG 420
Query: 427 VLTVSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASF 486
+ + D AF P S L +D ++ Y++S ++P AT+ KS + N AP +ASF
Sbjct: 421 SIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASF 480
Query: 487 SSRGPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSH---RKTLYNIISGTSMSCPHIT 546
SSRGP++I D++KPD++APGVEILAA+ P + S R+ Y+++SGTSM+CPH+
Sbjct: 481 SSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVA 540
Query: 547 GIAVYVKTFNPTWSPAAIKSALMTTASPMNAKFD--PEAEFAYGSGHVNPLKAVRPGLVY 606
G+A YVKTF+P WSP+ I+SA+MTTA PMNA EFAYGSGHV+P+ A+ PGLVY
Sbjct: 541 GVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVY 600
Query: 607 DANESDYVKFLCGQGYTTNIVRIITNDNSACT---SKNIGRVWDLNYPSFGLFVSHSKIF 666
+ ++D++ FLCG YT++ +RII+ DNS CT SK + R +LNYP+ VS +K F
Sbjct: 601 ELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPF 660
Query: 667 DQYFTRTLTSVASQASTYRATISTPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTM-- 726
+ F RT+T+V Q STY A + G LSIKV+P VLS + +++SF +TV
Sbjct: 661 NITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIG 720
Query: 727 RKYIVSASLVWSDGVHTVRSPITITSLS 731
K VSA+L+WSDG H VRSPI + ++S
Sbjct: 721 TKQPVSANLIWSDGTHNVRSPIIVYAMS 741
BLAST of Lsi04G009190 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 620.5 bits (1599), Expect = 2.4e-176
Identity = 344/742 (46.36%), Postives = 473/742 (63.75%), Query Frame = 0
Query: 4 SITMSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF 63
++ SSSL+ L+ L SS+ + DDK++Y+VYMG + D H +
Sbjct: 3 TLAASSSLLSCLL-------VLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNIL 62
Query: 64 -PFAPES-----VVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWD 123
ES +V +YKRSFNGFA +LT+ E E++A M GVVSVFPNK QL TT SWD
Sbjct: 63 QEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWD 122
Query: 124 FLGFPKNV--PRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTC 183
F+G + + R VE++ I+GV+DSGI PES SF DK FGP P KWKG C NFTC
Sbjct: 123 FMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTC 182
Query: 184 NRKIIGARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGV 243
N K+IGAR Y + RD +GHGTHTASTAAG + ASF+G+G GTVRGGV
Sbjct: 183 NNKLIGARDY---------TSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGV 242
Query: 244 PSARIAVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHA 303
P++R+A YK+C GCS LL+AFDDAIADGVD+I++S+G +T+ + D IAIG+FHA
Sbjct: 243 PASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHA 302
Query: 304 TQNGILTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTF 363
G+LT NSAGNSGPK + + ++PW+L+VAAST +R F+TKV +GN + G S+N +
Sbjct: 303 MAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAY 362
Query: 364 DMNGQ-YPLVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGA 423
+M G+ YPLV G ++ ++ C + V+ VKGKIL+C GA
Sbjct: 363 EMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGL-KIVESVGA 422
Query: 424 AGVLTVSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLA 483
G++ + D AF +PLP++ L D +++ Y+ S SP A + K+ + N ++P +A
Sbjct: 423 VGLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIA 482
Query: 484 SFSSRGPNAITKDLVKPDLSAPGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHIT 543
SFSSRGPN I D++KPD++APGVEILAA+ P P R Y+++SGTSMSCPH+
Sbjct: 483 SFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVA 542
Query: 544 GIAVYVKTFNPTWSPAAIKSALMTTASPMNAKFD--PEAEFAYGSGHVNPLKAVRPGLVY 603
G+A YVKTFNP WSP+ I+SA+MTTA P+NA EFAYGSGHV+P+ A PGLVY
Sbjct: 543 GVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVY 602
Query: 604 DANESDYVKFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQ 663
+ ++SD++ FLCG YT+ ++++I+ + C+ +LNYPS +S S F
Sbjct: 603 ELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTV 662
Query: 664 YFTRTLTSVASQASTYRATISTPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYI 723
F RTLT+V + STY + + +G L +K+ P+VLSF + +++SF +TV G+
Sbjct: 663 TFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSE 722
Query: 724 V--SASLVWSDGVHTVRSPITI 727
V SA+L+WSDG H VRSPI +
Sbjct: 723 VPSSANLIWSDGTHNVRSPIVV 727
BLAST of Lsi04G009190 vs. ExPASy TrEMBL
Match:
A0A1S3CFD6 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=3 SV=1)
HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 557/730 (76.30%), Postives = 619/730 (84.79%), Query Frame = 0
Query: 1 MTRSITMSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQS 60
M RS TMS SLI KLVF N+FF TLLASSLDS DDK+IY+VYMG+K KDD D A+ H S
Sbjct: 1 MRRSNTMSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGF 120
SFPFAPESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN HTTRSWDF+GF
Sbjct: 61 SFPFAPESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGF 120
Query: 121 PKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGA 180
+NVPRVKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGA
Sbjct: 121 SQNVPRVKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGA 180
Query: 181 RAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAV 240
R+Y IGR LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAV
Sbjct: 181 RSYHIGRPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAV 240
Query: 241 YKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILT 300
YK+CW D CSD D+LAAFDDAIADGVDIISLSVG +R YF+DSIAIGSFHA + GILT
Sbjct: 241 YKVCWRDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILT 300
Query: 301 SNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYP 360
SNSAGN+GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD GQYP
Sbjct: 301 SNSAGNNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYP 360
Query: 361 LVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTV-S 420
LV G V T F ++ S C NNSV+ KLVKGKILIC+ F GG AGVL + +
Sbjct: 361 LVTGRQVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDT 420
Query: 421 DTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGP 480
+ D A S P+PS+ LD NDA + YI+S SPTATIFKS N AP + SFSSRGP
Sbjct: 421 ELIDNARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGP 480
Query: 481 NAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKT 540
N ITK+++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKT
Sbjct: 481 NNITKEIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKT 540
Query: 541 FNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600
FNPTWSPAAIKSALMTTA PMNA + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKF
Sbjct: 541 FNPTWSPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKF 600
Query: 601 LCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVAS 660
LCGQGYTTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VAS
Sbjct: 601 LCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVAS 660
Query: 661 QASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHT 720
QASTYRA IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+
Sbjct: 661 QASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHS 720
Query: 721 VRSPITITSL 730
VRSPIT+TSL
Sbjct: 721 VRSPITVTSL 728
BLAST of Lsi04G009190 vs. ExPASy TrEMBL
Match:
A0A5A7UD73 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001640 PE=3 SV=1)
HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 553/724 (76.38%), Postives = 615/724 (84.94%), Query Frame = 0
Query: 7 MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 66
MS SLI KLVF N+FF TLLASSLDS DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 1 MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60
Query: 67 ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 126
ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN HTTRSWDF+GF +NVPR
Sbjct: 61 ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120
Query: 127 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 186
VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180
Query: 187 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 246
R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 240
Query: 247 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 306
D CSD D+LAAFDDAIADGVDIISLSVG +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 241 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 300
Query: 307 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 366
+GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD GQYPLV G
Sbjct: 301 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGRQ 360
Query: 367 VLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTV-SDTRDTA 426
V T F ++ S C NNSV+ KLVKGKILIC+ F GG AGVL + ++ D A
Sbjct: 361 VPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 420
Query: 427 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 486
S P+PS+ LD NDA + YI+S SPTATIFKS N AP + SFSSRGPN ITK+
Sbjct: 421 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 480
Query: 487 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 546
++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 481 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 540
Query: 547 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 606
PAAIKSALMTTA PMNA + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 541 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 600
Query: 607 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 666
TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 601 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 660
Query: 667 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 726
A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 661 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 720
Query: 727 ITSL 730
+TSL
Sbjct: 721 VTSL 722
BLAST of Lsi04G009190 vs. ExPASy TrEMBL
Match:
A0A1S4E3D9 (LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=3 SV=1)
HSP 1 Score: 1071.6 bits (2770), Expect = 1.4e-309
Identity = 545/730 (74.66%), Postives = 605/730 (82.88%), Query Frame = 0
Query: 1 MTRSITMSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQS 60
MTRS TMS SL+F LVFLN+FFSTLLAS+LDS D+ +KIY+VYMG+KLKDD DSA+ H S
Sbjct: 1 MTRSNTMSFSLLFNLVFLNLFFSTLLASTLDSDDNGRKIYIVYMGKKLKDDPDSANLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGF 120
SFPFAPESV+YTY RSFNGFAVKLTKEEA+KIA M+GVVSVFPN++N+LHTTRSWDF+GF
Sbjct: 61 SFPFAPESVIYTYNRSFNGFAVKLTKEEADKIAGMKGVVSVFPNEINKLHTTRSWDFMGF 120
Query: 121 PKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGA 180
P+NV RVKQV +N++VGV DSGIWPESPSF+DK F P PSKWKG+C A NFTCNRKIIGA
Sbjct: 121 PQNVRRVKQVGSNIVVGVFDSGIWPESPSFNDKGFDPPPSKWKGTCSAFNFTCNRKIIGA 180
Query: 181 RAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAV 240
RAY IGR LPHGDV+ PRDT+GHGTH AS A GGL+++AS GLGLGT RGG+PSARIAV
Sbjct: 181 RAYHIGRPLPHGDVEGPRDTDGHGTHCASIAPGGLVNKASLNGLGLGTARGGIPSARIAV 240
Query: 241 YKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILT 300
YKICWND +DLLAAFDDAI+DGVDIISLSVG SR YF D IAIGSFHA QN ILT
Sbjct: 241 YKICWND--DXMDLLAAFDDAISDGVDIISLSVGGNISRKYFRDPIAIGSFHAIQNNILT 300
Query: 301 SNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYP 360
SNSAGN GP ++T TSLSPWLLSVAASTMDRKF+TKVQIGNK S +GVSINTF GQYP
Sbjct: 301 SNSAGNWGPNVYTVTSLSPWLLSVAASTMDRKFVTKVQIGNKRSIQGVSINTFGTTGQYP 360
Query: 361 LVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVS- 420
LVA DV F N TS C NNSVN KLVKGKIL C+ F +FGG AGVL V+
Sbjct: 361 LVAARDVPNNGFDNLTSTYCFNNSVNLKLVKGKILFCESSFHPVLFSSFGGVAGVLMVNV 420
Query: 421 DTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGP 480
+ D A S PLPSS L+ +DA TIF YI S RSP A+I +S + N AP + SFSSRGP
Sbjct: 421 NPLDDALSFPLPSSVLNFHDAITIFDYIRSTRSPNASILRSTAVRNEPAPVVVSFSSRGP 480
Query: 481 NAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKT 540
N +TK+++KPDLS PGVEILAAWPPVAPVG +R TLYNIISGTSMSCPHIT IA YVKT
Sbjct: 481 NNLTKEIIKPDLSGPGVEILAAWPPVAPVGEINRNTLYNIISGTSMSCPHITEIAAYVKT 540
Query: 541 FNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600
FNPTWSPAAIKSALMTTA PMNA + EAEFAYGSGHVNP +A+RPGLVYDANE DY+KF
Sbjct: 541 FNPTWSPAAIKSALMTTALPMNATLNLEAEFAYGSGHVNPKRAIRPGLVYDANEIDYIKF 600
Query: 601 LCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVAS 660
LCGQGYT +V IIT+ AC S NIGRVWDLNYPSFGL VSHSK F QYF RTLTSVAS
Sbjct: 601 LCGQGYTNGMVEIITSYEDACNSSNIGRVWDLNYPSFGLSVSHSKTFKQYFRRTLTSVAS 660
Query: 661 QASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHT 720
QAS Y+A IS P GL I VNPNVLSFNGIGD+KSFKL VRGT+++ IVSASLVWSDGVH+
Sbjct: 661 QASKYKAMISAPRGLVITVNPNVLSFNGIGDKKSFKLKVRGTIKESIVSASLVWSDGVHS 720
Query: 721 VRSPITITSL 730
VRSPITI SL
Sbjct: 721 VRSPITINSL 728
BLAST of Lsi04G009190 vs. ExPASy TrEMBL
Match:
A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)
HSP 1 Score: 1068.9 bits (2763), Expect = 9.3e-309
Identity = 543/732 (74.18%), Postives = 605/732 (82.65%), Query Frame = 0
Query: 7 MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSF---- 66
MSSSLIFKL F ++FFS LAS LDS DD K IY+VYMGRKL +D DSAH H +
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60
Query: 67 ---PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLG 126
FAPESV++TYKRSFNGFAVKLT+EEAEKIA MEGVVSVF N+MN+LHTTRSWDFLG
Sbjct: 61 VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
Query: 127 FPKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKII 186
FP VPR QVE+N++VGVLD+GIWPESPSFDD+ F P P KWKG+C+ + NF CNRKII
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
Query: 187 GARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARI 246
GAR+Y IGR + GDV+ PRDTNGHGTHTASTAAGGL+SQA+ YGLGLGT RGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
Query: 247 AVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGI 306
A YK+CWNDGCSD D+LAA+DDAIADGVDIISLSVG R YF+D+IAIGSFHA + GI
Sbjct: 241 AAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300
Query: 307 LTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQ 366
LTSNSAGN GP FTT SLSPWLLSVAASTMDRKF+T+VQIGN SF+GVSINTFD N
Sbjct: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQY 360
Query: 367 YPLVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFF-NFGGAAGVLT 426
YPLV+G D+ F STSR C +NSV PKL+KGKI++C+ +FF + GAAGVL
Sbjct: 361 YPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420
Query: 427 VSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSR 486
S+TRD A S PLPSS LDPND YI+S RSP ATIFKS T+ N SAP + SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480
Query: 487 GPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYV 546
GPN TKD++KPD+S PGVEILAAWP VAPVGG R TL+NIISGTSMSCPHITGIA YV
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYV 540
Query: 547 KTFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYV 606
KT+NPTWSPAAIKSALMTTASPMNA+F+P+AEFAYGSGHVNPLKAVRPGLVYDANESDYV
Sbjct: 541 KTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYV 600
Query: 607 KFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSV 666
KFLCGQGY T VR IT D SACT N GRVWDLNYPSFGL VS SK F+QYF RTLTSV
Sbjct: 601 KFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSV 660
Query: 667 ASQASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGV 726
A QASTYRA IS P GL+I VNPNVLSFNG+GDRKSF LTVRG+++ ++VSASLVWSDGV
Sbjct: 661 APQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGV 720
Query: 727 HTVRSPITITSL 730
H+VRSPITITSL
Sbjct: 721 HSVRSPITITSL 730
BLAST of Lsi04G009190 vs. ExPASy TrEMBL
Match:
A0A5D3E4N6 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00100 PE=3 SV=1)
HSP 1 Score: 1063.5 bits (2749), Expect = 3.9e-307
Identity = 538/724 (74.31%), Postives = 604/724 (83.43%), Query Frame = 0
Query: 7 MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 66
MS SLI KLVF N+FF TLLASSLDS DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 1 MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60
Query: 67 ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 126
ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN HTTRSWDF+GF +NVPR
Sbjct: 61 ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120
Query: 127 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 186
VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180
Query: 187 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 246
R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 240
Query: 247 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 306
D CSD D+LAAFDDAIADGVDIISLSVG +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 241 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 300
Query: 307 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 366
+GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+ + + F + +
Sbjct: 301 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQVILLIFF--------ILFFS 360
Query: 367 VLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTV-SDTRDTA 426
L F + + C NNSV+ KLVKGKILIC+ F GG AGVL + ++ D A
Sbjct: 361 KLYLIFVKTWNSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 420
Query: 427 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 486
S P+PS+ LD NDA + YI+S SPTATIFKS N AP + SFSSRGPN ITK+
Sbjct: 421 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 480
Query: 487 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 546
++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 481 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 540
Query: 547 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 606
PAAIKSALMTTA PMNA + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 541 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 600
Query: 607 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 666
TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 601 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 660
Query: 667 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 726
A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 661 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 714
Query: 727 ITSL 730
+TSL
Sbjct: 721 VTSL 714
BLAST of Lsi04G009190 vs. NCBI nr
Match:
XP_038892403.1 (cucumisin-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 581/731 (79.48%), Postives = 630/731 (86.18%), Query Frame = 0
Query: 1 MTRSITMSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQS 60
MTRS M LIFKL FL++FFSTLLASSLDS DDDKKIY+VYMGRK+KDD DSAH H S
Sbjct: 1 MTRSTAMYFCLIFKLFFLSLFFSTLLASSLDS-DDDKKIYIVYMGRKIKDDPDSAHLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGF 120
SFPFAPESVVY YKRSFNGFAVKLTKEEAEKIA MEGVVSVFPNK+N+LHTTRSWDF+ F
Sbjct: 61 SFPFAPESVVYMYKRSFNGFAVKLTKEEAEKIASMEGVVSVFPNKINKLHTTRSWDFMNF 120
Query: 121 PKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGA 180
PKNVPRVKQVE+N++VGV D+GIWPESPSF+DK FGP PSKWKG+C NFTCNRKIIGA
Sbjct: 121 PKNVPRVKQVESNIVVGVFDTGIWPESPSFNDKGFGPPPSKWKGTCLVFNFTCNRKIIGA 180
Query: 181 RAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAV 240
RAY IGR LP G+V+SPRDTNGHGTHTASTAAGGL+S+AS YGLGLGT RGGVPSARIA
Sbjct: 181 RAYHIGRPLPRGEVESPRDTNGHGTHTASTAAGGLVSKASLYGLGLGTARGGVPSARIAA 240
Query: 241 YKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILT 300
YKICW+D CSD+D+LAAFDDAIADGVDIISLSVG SR YF D IAIGSFHA Q GILT
Sbjct: 241 YKICWSDSCSDLDILAAFDDAIADGVDIISLSVGGNESRQYFRDPIAIGSFHAMQKGILT 300
Query: 301 SNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYP 360
SNSAGN GP+ FTTTSLSPWLLSVAAST DRKF+TKVQIGNKNSF+GVSINTFD GQYP
Sbjct: 301 SNSAGNDGPRYFTTTSLSPWLLSVAASTTDRKFVTKVQIGNKNSFQGVSINTFDTKGQYP 360
Query: 361 LVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSD 420
LVAG D+ FHNSTSR C NNSV+PKLVKGKI+ C+ + +F + GGA GVL D
Sbjct: 361 LVAGRDIPNKGFHNSTSRYCFNNSVDPKLVKGKIVFCETNVDSSEFISLGGAMGVLGKGD 420
Query: 421 --TRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRG 480
T D FS PLPSSTLD +DA I HYI + R PTATIFKS HN +P + SFSSRG
Sbjct: 421 KNTMDCEFSYPLPSSTLDVDDATIISHYIDNTRFPTATIFKSTATHNAPSPVVVSFSSRG 480
Query: 481 PNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVK 540
PNA TKDL+KPDLSAPGVEILAAWPPVAPVGG R TLYNIISGTSMSCPH+TGIA YVK
Sbjct: 481 PNAATKDLIKPDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVTGIAAYVK 540
Query: 541 TFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVK 600
TFNPTWSPAAIKSALMTTASPMN++F+P+AEFAYGSGHVNPLKA+ PGLVYDANE+DYVK
Sbjct: 541 TFNPTWSPAAIKSALMTTASPMNSQFNPQAEFAYGSGHVNPLKALTPGLVYDANETDYVK 600
Query: 601 FLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVA 660
FLCGQGYTT++VRIITNDNS C S N GRVWDLNYPSFGL VSHSK F+QYFTRTLTSVA
Sbjct: 601 FLCGQGYTTDLVRIITNDNSVCISTNTGRVWDLNYPSFGLSVSHSKTFNQYFTRTLTSVA 660
Query: 661 SQASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVH 720
S ASTY+A IS P GL+I V P VLSFNG GD KSFKLTVRGTMR+ IVSASL+WSD VH
Sbjct: 661 SGASTYKAMISAPKGLAITVKPKVLSFNGTGDMKSFKLTVRGTMRESIVSASLIWSDSVH 720
Query: 721 TVRSPITITSL 730
TVRSPITITSL
Sbjct: 721 TVRSPITITSL 730
BLAST of Lsi04G009190 vs. NCBI nr
Match:
XP_008461722.1 (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 557/730 (76.30%), Postives = 619/730 (84.79%), Query Frame = 0
Query: 1 MTRSITMSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQS 60
M RS TMS SLI KLVF N+FF TLLASSLDS DDK+IY+VYMG+K KDD D A+ H S
Sbjct: 1 MRRSNTMSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGF 120
SFPFAPESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN HTTRSWDF+GF
Sbjct: 61 SFPFAPESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGF 120
Query: 121 PKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGA 180
+NVPRVKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGA
Sbjct: 121 SQNVPRVKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGA 180
Query: 181 RAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAV 240
R+Y IGR LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAV
Sbjct: 181 RSYHIGRPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAV 240
Query: 241 YKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILT 300
YK+CW D CSD D+LAAFDDAIADGVDIISLSVG +R YF+DSIAIGSFHA + GILT
Sbjct: 241 YKVCWRDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILT 300
Query: 301 SNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYP 360
SNSAGN+GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD GQYP
Sbjct: 301 SNSAGNNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYP 360
Query: 361 LVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTV-S 420
LV G V T F ++ S C NNSV+ KLVKGKILIC+ F GG AGVL + +
Sbjct: 361 LVTGRQVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDT 420
Query: 421 DTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGP 480
+ D A S P+PS+ LD NDA + YI+S SPTATIFKS N AP + SFSSRGP
Sbjct: 421 ELIDNARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGP 480
Query: 481 NAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKT 540
N ITK+++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKT
Sbjct: 481 NNITKEIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKT 540
Query: 541 FNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600
FNPTWSPAAIKSALMTTA PMNA + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKF
Sbjct: 541 FNPTWSPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKF 600
Query: 601 LCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVAS 660
LCGQGYTTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VAS
Sbjct: 601 LCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVAS 660
Query: 661 QASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHT 720
QASTYRA IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+
Sbjct: 661 QASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHS 720
Query: 721 VRSPITITSL 730
VRSPIT+TSL
Sbjct: 721 VRSPITVTSL 728
BLAST of Lsi04G009190 vs. NCBI nr
Match:
KAA0051615.1 (cucumisin-like [Cucumis melo var. makuwa])
HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 553/724 (76.38%), Postives = 615/724 (84.94%), Query Frame = 0
Query: 7 MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 66
MS SLI KLVF N+FF TLLASSLDS DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 1 MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60
Query: 67 ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 126
ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN HTTRSWDF+GF +NVPR
Sbjct: 61 ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120
Query: 127 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 186
VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180
Query: 187 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 246
R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 240
Query: 247 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 306
D CSD D+LAAFDDAIADGVDIISLSVG +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 241 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 300
Query: 307 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 366
+GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD GQYPLV G
Sbjct: 301 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGRQ 360
Query: 367 VLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTV-SDTRDTA 426
V T F ++ S C NNSV+ KLVKGKILIC+ F GG AGVL + ++ D A
Sbjct: 361 VPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 420
Query: 427 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 486
S P+PS+ LD NDA + YI+S SPTATIFKS N AP + SFSSRGPN ITK+
Sbjct: 421 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 480
Query: 487 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 546
++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 481 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 540
Query: 547 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 606
PAAIKSALMTTA PMNA + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 541 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 600
Query: 607 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 666
TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 601 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 660
Query: 667 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 726
A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 661 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 720
Query: 727 ITSL 730
+TSL
Sbjct: 721 VTSL 722
BLAST of Lsi04G009190 vs. NCBI nr
Match:
XP_038892506.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 556/738 (75.34%), Postives = 618/738 (83.74%), Query Frame = 0
Query: 1 MTRSITMSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQS 60
M RS MSSSL+FKL F++ FFST+L SS +S DD KKIY+VYMGRKL +D DSAH H
Sbjct: 1 MIRSTAMSSSLVFKLFFVSFFFSTILTSSFESDDDGKKIYIVYMGRKL-EDPDSAHLHHR 60
Query: 61 SF-------PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTR 120
+ FAPESV+Y+YKRSFNGF VKLT+EEAEKIA MEGVVSVF N+MN LHTTR
Sbjct: 61 AMLEQVVGSNFAPESVLYSYKRSFNGFVVKLTEEEAEKIASMEGVVSVFLNEMNVLHTTR 120
Query: 121 SWDFLGFPKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTC 180
SWDFL FP+N+ RV QVE+N++VGVLDSGIWPESPSF+DK F PSKWKGSCQA NFTC
Sbjct: 121 SWDFLNFPQNIQRVNQVESNIVVGVLDSGIWPESPSFNDKGFDSPPSKWKGSCQAFNFTC 180
Query: 181 NRKIIGARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGV 240
NRKIIG RAY IG L GDV+SPRDT+GHGTHTASTAAGGL+SQA+ Y LGLGT RGGV
Sbjct: 181 NRKIIGGRAYHIGGPLRPGDVNSPRDTDGHGTHTASTAAGGLVSQANLYRLGLGTARGGV 240
Query: 241 PSARIAVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHA 300
P ARIAVYKICW DGCSD D+LAA+DDAIADGVDIISLSVG R YF D IAIGSFHA
Sbjct: 241 PLARIAVYKICWKDGCSDADILAAYDDAIADGVDIISLSVGANKPRQYFSDPIAIGSFHA 300
Query: 301 TQNGILTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTF 360
+ GILTSNSAGN GP FTTTSLSPWLLSVAAST+DRKF+T+VQIGN SF+GVSINTF
Sbjct: 301 IEKGILTSNSAGNEGPNFFTTTSLSPWLLSVAASTIDRKFVTQVQIGNGQSFQGVSINTF 360
Query: 361 DMNGQYPLVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFF-NFGGA 420
+MNGQYPLV G D+ F +STSR C N SVNP L++GKI++C+ +FF + GA
Sbjct: 361 EMNGQYPLVLGRDIPNIGFDSSTSRYCFNKSVNPYLLRGKIVLCEASFGPAEFFKSLDGA 420
Query: 421 AGVLTVSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLA 480
AGVL ++ TRD A S P PSSTLDPNDA IF YI+S PTATIFKS + N SAP +
Sbjct: 421 AGVLMLASTRDHASSYPFPSSTLDPNDATDIFRYIYSTSFPTATIFKSTAILNTSAPVVV 480
Query: 481 SFSSRGPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITG 540
SFSSRGPNA TKDL+KPDLSAPGVEILAAWPPVAPVGG R TLYNIISGTSMSCPH+TG
Sbjct: 481 SFSSRGPNAATKDLIKPDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVTG 540
Query: 541 IAVYVKTFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDAN 600
IA YVKTFNPTWSPAAIKSALMTTASPMNA+F P+ EFAYGSGHVNPLKAVRPGLVYDAN
Sbjct: 541 IAAYVKTFNPTWSPAAIKSALMTTASPMNARFHPQGEFAYGSGHVNPLKAVRPGLVYDAN 600
Query: 601 ESDYVKFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTR 660
ESDYVKFLCGQGY+T++VR IT D SACTS NIGRVWDLNYPSFGL VS S+ F QYFTR
Sbjct: 601 ESDYVKFLCGQGYSTSMVRRITGDYSACTSGNIGRVWDLNYPSFGLSVSGSQTFSQYFTR 660
Query: 661 TLTSVASQASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLV 720
TLTSVASQASTYRA IS P GL+I VNPNVLSFNGIGD+KSF LT+RG++ +Y+VSASLV
Sbjct: 661 TLTSVASQASTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFTLTIRGSVNQYVVSASLV 720
Query: 721 WSDGVHTVRSPITITSLS 731
W+DGVHTVRSPIT+T+LS
Sbjct: 721 WTDGVHTVRSPITVTTLS 737
BLAST of Lsi04G009190 vs. NCBI nr
Match:
XP_038892404.1 (cucumisin-like isoform X2 [Benincasa hispida])
HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 533/669 (79.67%), Postives = 578/669 (86.40%), Query Frame = 0
Query: 63 PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPK 122
PFAPESVVY YKRSFNGFAVKLTKEEAEKIA MEGVVSVFPNK+N+LHTTRSWDF+ FPK
Sbjct: 5 PFAPESVVYMYKRSFNGFAVKLTKEEAEKIASMEGVVSVFPNKINKLHTTRSWDFMNFPK 64
Query: 123 NVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARA 182
NVPRVKQVE+N++VGV D+GIWPESPSF+DK FGP PSKWKG+C NFTCNRKIIGARA
Sbjct: 65 NVPRVKQVESNIVVGVFDTGIWPESPSFNDKGFGPPPSKWKGTCLVFNFTCNRKIIGARA 124
Query: 183 YRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYK 242
Y IGR LP G+V+SPRDTNGHGTHTASTAAGGL+S+AS YGLGLGT RGGVPSARIA YK
Sbjct: 125 YHIGRPLPRGEVESPRDTNGHGTHTASTAAGGLVSKASLYGLGLGTARGGVPSARIAAYK 184
Query: 243 ICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSN 302
ICW+D CSD+D+LAAFDDAIADGVDIISLSVG SR YF D IAIGSFHA Q GILTSN
Sbjct: 185 ICWSDSCSDLDILAAFDDAIADGVDIISLSVGGNESRQYFRDPIAIGSFHAMQKGILTSN 244
Query: 303 SAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLV 362
SAGN GP+ FTTTSLSPWLLSVAAST DRKF+TKVQIGNKNSF+GVSINTFD GQYPLV
Sbjct: 245 SAGNDGPRYFTTTSLSPWLLSVAASTTDRKFVTKVQIGNKNSFQGVSINTFDTKGQYPLV 304
Query: 363 AGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSD-- 422
AG D+ FHNSTSR C NNSV+PKLVKGKI+ C+ + +F + GGA GVL D
Sbjct: 305 AGRDIPNKGFHNSTSRYCFNNSVDPKLVKGKIVFCETNVDSSEFISLGGAMGVLGKGDKN 364
Query: 423 TRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPN 482
T D FS PLPSSTLD +DA I HYI + R PTATIFKS HN +P + SFSSRGPN
Sbjct: 365 TMDCEFSYPLPSSTLDVDDATIISHYIDNTRFPTATIFKSTATHNAPSPVVVSFSSRGPN 424
Query: 483 AITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTF 542
A TKDL+KPDLSAPGVEILAAWPPVAPVGG R TLYNIISGTSMSCPH+TGIA YVKTF
Sbjct: 425 AATKDLIKPDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVTGIAAYVKTF 484
Query: 543 NPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFL 602
NPTWSPAAIKSALMTTASPMN++F+P+AEFAYGSGHVNPLKA+ PGLVYDANE+DYVKFL
Sbjct: 485 NPTWSPAAIKSALMTTASPMNSQFNPQAEFAYGSGHVNPLKALTPGLVYDANETDYVKFL 544
Query: 603 CGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQ 662
CGQGYTT++VRIITNDNS C S N GRVWDLNYPSFGL VSHSK F+QYFTRTLTSVAS
Sbjct: 545 CGQGYTTDLVRIITNDNSVCISTNTGRVWDLNYPSFGLSVSHSKTFNQYFTRTLTSVASG 604
Query: 663 ASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTV 722
ASTY+A IS P GL+I V P VLSFNG GD KSFKLTVRGTMR+ IVSASL+WSD VHTV
Sbjct: 605 ASTYKAMISAPKGLAITVKPKVLSFNGTGDMKSFKLTVRGTMRESIVSASLIWSDSVHTV 664
Query: 723 RSPITITSL 730
RSPITITSL
Sbjct: 665 RSPITITSL 673
BLAST of Lsi04G009190 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 632.9 bits (1631), Expect = 3.3e-181
Identity = 349/721 (48.40%), Postives = 466/721 (64.63%), Query Frame = 0
Query: 25 LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 84
LL+S D+D ++Y+VYMG + D H S ES +V +YKRSF
Sbjct: 18 LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77
Query: 85 NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 144
NGFA +LT+ E IA++EGVVSVFPNK+ QLHTT SWDF+G KN R +E++ I
Sbjct: 78 NGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137
Query: 145 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 204
+GV+D+GIWPES SF DK FGP P KWKG C NFTCN K+IGAR Y
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197
Query: 205 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 264
+ RDT+GHGTHTASTAAG + SF+G+G GTVRGGVP++RIA YK+C + GCS L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257
Query: 265 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 324
L++FDDAIADGVD+I++S+GF+ + D IAIG+FHA GILT +SAGNSGPK T
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317
Query: 325 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 384
+ ++PW+ +VAAST +R FITKV +GN + G S+N FDM G +YPLV G ++
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377
Query: 385 NSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSDTRDTAFSNPLPSS 444
T+ C +N VKGKIL+C Y GA ++ S D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGY-KIAKSVGAIAIIDKSPRPDVAFTHHLPAS 437
Query: 445 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 504
L D ++ YI S SP A + K+ T+ N ++P +ASFSSRGPN I D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497
Query: 505 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 564
PGVEILAA+ P P R+ Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557
Query: 565 LMTTASPMNAKFD--PEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 624
+MTTA P+ AK EFAYG+GHV+P+ A+ PGLVY+ +++D++ FLCG YT+ +
Sbjct: 558 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 617
Query: 625 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 684
+II+ D C+ KN +LNYPS +S + F F RTLT+V + STY++ +
Sbjct: 618 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 677
Query: 685 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 727
+G LSIKV P+VL F + +++SF +TV G+ V SA+L+WSDG H VRSPI
Sbjct: 678 AGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIV 728
BLAST of Lsi04G009190 vs. TAIR 10
Match:
AT5G59090.2 (subtilase 4.12 )
HSP 1 Score: 627.9 bits (1618), Expect = 1.0e-179
Identity = 346/719 (48.12%), Postives = 463/719 (64.39%), Query Frame = 0
Query: 25 LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 84
LL+S D+D ++Y+VYMG + D H S ES +V +YKRSF
Sbjct: 18 LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77
Query: 85 NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 144
NGFA +LT+ E IA++EGVVSVFPNK+ QLHTT SWDF+G KN R +E++ I
Sbjct: 78 NGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137
Query: 145 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 204
+GV+D+GIWPES SF DK FGP P KWKG C NFTCN K+IGAR Y
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197
Query: 205 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 264
+ RDT+GHGTHTASTAAG + SF+G+G GTVRGGVP++RIA YK+C + GCS L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257
Query: 265 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 324
L++FDDAIADGVD+I++S+GF+ + D IAIG+FHA GILT +SAGNSGPK T
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317
Query: 325 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 384
+ ++PW+ +VAAST +R FITKV +GN + G S+N FDM G +YPLV G ++
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377
Query: 385 NSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSDTRDTAFSNPLPSS 444
T+ C +N VKGKIL+C Y GA ++ S D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGY-KIAKSVGAIAIIDKSPRPDVAFTHHLPAS 437
Query: 445 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 504
L D ++ YI S SP A + K+ T+ N ++P +ASFSSRGPN I D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497
Query: 505 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 564
PGVEILAA+ P P R+ Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557
Query: 565 LMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNIVRI 624
+MTTA + EFAYG+GHV+P+ A+ PGLVY+ +++D++ FLCG YT+ ++I
Sbjct: 558 IMTTA---KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKI 617
Query: 625 ITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATISTP 684
I+ D C+ KN +LNYPS +S + F F RTLT+V + STY++ +
Sbjct: 618 ISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAG 677
Query: 685 NG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPITI 727
+G LSIKV P+VL F + +++SF +TV G+ V SA+L+WSDG H VRSPI +
Sbjct: 678 HGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVV 723
BLAST of Lsi04G009190 vs. TAIR 10
Match:
AT5G59090.3 (subtilase 4.12 )
HSP 1 Score: 626.7 bits (1615), Expect = 2.3e-179
Identity = 349/721 (48.40%), Postives = 464/721 (64.36%), Query Frame = 0
Query: 25 LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 84
LL+S D+D ++Y+VYMG + D H S ES +V +YKRSF
Sbjct: 18 LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77
Query: 85 NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 144
NGFA +LT+ E IA EGVVSVFPNK+ QLHTT SWDF+G KN R +E++ I
Sbjct: 78 NGFAARLTESERTLIA--EGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137
Query: 145 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 204
+GV+D+GIWPES SF DK FGP P KWKG C NFTCN K+IGAR Y
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197
Query: 205 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 264
+ RDT+GHGTHTASTAAG + SF+G+G GTVRGGVP++RIA YK+C + GCS L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257
Query: 265 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 324
L++FDDAIADGVD+I++S+GF+ + D IAIG+FHA GILT +SAGNSGPK T
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317
Query: 325 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 384
+ ++PW+ +VAAST +R FITKV +GN + G S+N FDM G +YPLV G ++
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377
Query: 385 NSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSDTRDTAFSNPLPSS 444
T+ C +N VKGKIL+C Y GA ++ S D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGY-KIAKSVGAIAIIDKSPRPDVAFTHHLPAS 437
Query: 445 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 504
L D ++ YI S SP A + K+ T+ N ++P +ASFSSRGPN I D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497
Query: 505 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 564
PGVEILAA+ P P R+ Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557
Query: 565 LMTTASPMNAKFD--PEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 624
+MTTA P+ AK EFAYG+GHV+P+ A+ PGLVY+ +++D++ FLCG YT+ +
Sbjct: 558 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 617
Query: 625 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 684
+II+ D C+ KN +LNYPS +S + F F RTLT+V + STY++ +
Sbjct: 618 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 677
Query: 685 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 727
+G LSIKV P+VL F + +++SF +TV G+ V SA+L+WSDG H VRSPI
Sbjct: 678 AGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIV 726
BLAST of Lsi04G009190 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 622.1 bits (1603), Expect = 5.8e-178
Identity = 343/748 (45.86%), Postives = 484/748 (64.71%), Query Frame = 0
Query: 7 MSSSLIFKLVFLNIFFSTLLASSLDSAD-DDKKIYVVYMGR-KLKDDRDSAHSHQSSF-- 66
M+ F +F ++ +L + S D D D+++Y+VY+G +++ H S
Sbjct: 1 MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60
Query: 67 ----PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFL 126
+V +YK+SFNGFA +LT+ E +++A ME VVSVFP++ +L TT SW+F+
Sbjct: 61 ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120
Query: 127 GFPKNV--PRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNR 186
G + + R + +E++ I+GV+DSGI+PES SF D+ FGP P KWKG+C NFTCN
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180
Query: 187 KIIGARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPS 246
K+IGAR Y +S + + RD +GHGTHTAS AAG ++ ++FYGLG GT RGGVP+
Sbjct: 181 KVIGARDY-TAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPA 240
Query: 247 ARIAVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQ 306
ARIAVYK+C N+GC +++AFDDAIADGVD+IS+S+ + P+ D IAIG+FHA
Sbjct: 241 ARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMA 300
Query: 307 NGILTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDM 366
G+LT N+AGN+GPK+ T TS +PW+ SVAAS +R F+ KV +G+ G S+NT+DM
Sbjct: 301 VGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM 360
Query: 367 NG-QYPLVAGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAG 426
NG YPLV G ++ +R C ++ KLVKGKI++C + GA G
Sbjct: 361 NGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL-GAVG 420
Query: 427 VLTVSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASF 486
+ + D AF P S L +D ++ Y++S ++P AT+ KS + N AP +ASF
Sbjct: 421 SIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASF 480
Query: 487 SSRGPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSH---RKTLYNIISGTSMSCPHIT 546
SSRGP++I D++KPD++APGVEILAA+ P + S R+ Y+++SGTSM+CPH+
Sbjct: 481 SSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVA 540
Query: 547 GIAVYVKTFNPTWSPAAIKSALMTTASPMNAKFD--PEAEFAYGSGHVNPLKAVRPGLVY 606
G+A YVKTF+P WSP+ I+SA+MTTA PMNA EFAYGSGHV+P+ A+ PGLVY
Sbjct: 541 GVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVY 600
Query: 607 DANESDYVKFLCGQGYTTNIVRIITNDNSACT---SKNIGRVWDLNYPSFGLFVSHSKIF 666
+ ++D++ FLCG YT++ +RII+ DNS CT SK + R +LNYP+ VS +K F
Sbjct: 601 ELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPF 660
Query: 667 DQYFTRTLTSVASQASTYRATISTPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTM-- 726
+ F RT+T+V Q STY A + G LSIKV+P VLS + +++SF +TV
Sbjct: 661 NITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIG 720
Query: 727 RKYIVSASLVWSDGVHTVRSPITITSLS 731
K VSA+L+WSDG H VRSPI + ++S
Sbjct: 721 TKQPVSANLIWSDGTHNVRSPIIVYAMS 741
BLAST of Lsi04G009190 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 620.9 bits (1600), Expect = 1.3e-177
Identity = 329/676 (48.67%), Postives = 455/676 (67.31%), Query Frame = 0
Query: 65 APESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNV 124
A +V +YKRSFNGFA L++ E++K+ +M+ VVSVFP+K ++L TTRSWDF+GF +
Sbjct: 28 ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKA 87
Query: 125 PRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAY 184
R E++VIVGV+DSGIWPES SFDD+ FGP P KWKGSC+ + F CN K+IGAR Y
Sbjct: 88 RRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY 147
Query: 185 RIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKI 244
+ DS RD GHGTHTASTAAG + ASFYGL GT RGGVPSARIA YK+
Sbjct: 148 -------NKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKV 207
Query: 245 CWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNS 304
C+N C+D+D+LAAFDDAIADGVD+IS+S+ + S+AIGSFHA GI+T+ S
Sbjct: 208 CFN-RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGS 267
Query: 305 AGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLV 364
AGN+GP + ++SPW+++VAAS DR+FI +V +GN + G+S+NTF++NG ++P+V
Sbjct: 268 AGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIV 327
Query: 365 AGYDVLKTSFHNSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGAAGVLTVSD-T 424
G +V + + + + C + V+ +LVKGKI++C + L Y + + GA GV+ +
Sbjct: 328 YGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAY-LAGAIGVIVQNTLL 387
Query: 425 RDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNA 484
D+AF P P+S+L D +I YI SA P A I ++ + + AP++ SFSSRGP+
Sbjct: 388 PDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSF 447
Query: 485 ITKDLVKPDLSAPGVEILAAWPPVAPVGG-----SHRKTLYNIISGTSMSCPHITGIAVY 544
+ ++L+KPD+SAPG+EILAA+ PVA R Y+++SGTSM+CPH+ G+A Y
Sbjct: 448 VIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAY 507
Query: 545 VKTFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNPLKAVRPGLVYDANESDY 604
VK+F+P WSP+AIKSA+MTTA+PMN K +PE EFAYGSG +NP KA PGLVY+ DY
Sbjct: 508 VKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDY 567
Query: 605 VKFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTS 664
+K LC +G+ + + + N C+ + V DLNYP+ FVS F+ F RT+T+
Sbjct: 568 LKMLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTN 627
Query: 665 VASQASTYRAT-ISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRK--YIVSASLVW 724
V STY+A+ + L I + P +L F + ++KSF +T+ G K VS+S+VW
Sbjct: 628 VGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVW 687
Query: 725 SDGVHTVRSPITITSL 730
SDG H+VRSPI S+
Sbjct: 688 SDGSHSVRSPIVAYSI 691
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 2.5e-311 | 74.18 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q8L7D2 | 4.6e-180 | 48.40 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Q9FIF8 | 2.5e-178 | 47.57 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FGU3 | 8.1e-177 | 45.86 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
| Q9FIG2 | 2.4e-176 | 46.36 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S3CFD6 | 0.0e+00 | 76.30 | cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=3 SV=1 | [more] |
| A0A5A7UD73 | 0.0e+00 | 76.38 | Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00164... | [more] |
| A0A1S4E3D9 | 1.4e-309 | 74.66 | LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=3... | [more] |
| A0A1S3CF86 | 9.3e-309 | 74.18 | LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1 | [more] |
| A0A5D3E4N6 | 3.9e-307 | 74.31 | Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00100... | [more] |
