Lsi04G008770 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G008770
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr04: 8623955 .. 8628065 (+)
RNA-Seq Expression	Lsi04G008770
Synteny	Lsi04G008770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAGAAGGTGAGGCTGTCATCTTTCGATCTTATCCTCACCCGTGGATATCTTTTTTACTCACACGCCGTCGATTTTCCACCTCAGATTTGATGACCTGGCAATATCCCATTGGTCCGTCTCGTCAAACCTGACACCCACAGGTCACCACCACCACCTTCGCAACACTTTCTCTCTTTCCGTGTTCGCATCGCTATATAAATATTCCCCACTTTCCCCTGTTTCAGAATCCCGTCCCAGTGGTCCTCTCTTCCCTCGCCCCACCCATTTCTCCACCGCCCTCCGACCATGTCCGCCGCCGCATTTCGCCGTCTCTCCGCGGCGCTTTTCCTCCTTCTCCAAATCTCCACCGCCGTCGTCGCCATCGGCGTTAACTACGGCACTCTAGCCAACAACCTCCGCCCGCCGGCGGAGGTGGTGAACTTCCTCAAAACAAAAACAAAAATCGACCGCGTGAAGATCTTCGATACAAATCCGGATATCCTAAGAGCCTTCGCCGGTTCCGGAATCCTGGTGACGGTGACTGTCGGCAACGGCGAGATCCCCGCTCTGGGGGATCTGGCCGCCGCTCGGCAGTGGGTAGCCGCCCATATCGCGCCGTTTCACCCTCAGACGCGGATTAATTACATTGCTGTGGGGAATGAAATCATACACACTAACGATAAGCCTCTGATCGCTCGTCTTGTTCCCGCCATGAGATCGCTCCACCAAGCTCTGGTTCTCGCCGGAATTAAGGACATTAAGGTAATTATCTTGATTTGTTGCTTCTCATTTGCTTTTTTCGCCGTTCATGGAAAAGCCGTTTTAAAGCTGTTGGGTTGTAATGATTTATTCCTTTGAGTGATTTAATTTAAATTAATGATTGAAGTTTTTAAAAAATGACTACATCTATTTCAAAGATTTCTAATTCGTATTACAAAACAACGATAAATCATTTCCAAAATTTAAAATTATATAATCTTTTCTTTAATTTTTGTTGATTTCTATACAACTCACGCATCATGAATAGATTTTTTTTTTTAAATCTAATTTATAACAAGTATTATGTTAGGCTCATTTTAACCATATAAATAGAAATTTATTAATTTTAAAATTAGAGATTCGAATCCAATAGCCTATTTATTTTATTAGAAGTTTGAGTAATTTTACTTGAATTGTGTTGTTTGTTCTCATTTAAAATAAGAAGTGATTTAAGTTTATCATATGTATTTTTTAAAAAATAAAAATCACTACATTTACATGTATTTTTAAATATATATATACATTTTATTTTTTTTCAAATATTATAATTACGAACATAAGTATAGTTTTACTTTGTATTATGGATTTCAAAATTCTTGAATTCCATTTCTTATCATTTATATTGTTAAAAATATGCACTTTATTTTAATTTTAATTTGATTTGATGGTGTAAAATTTGTAAAACAATACAAAATGGTTAATTAATTTTCATAAAATTTACATAGCTTTAAAATAAACTTCGTAACTTCTTTTATTAATACATGAACACCAAAAGTATCCAATAGACTTTCTATTTATTTTGTTCATTATATTATCATATACGAACTTTTGCAAAATATTGATCTAATCATCGAGGTTTTAAAAGTATTAAACTTTAGTTATTAAAATTTAAAGATTGTACTATTTTAATTTAATTTAATTAATGATGTAGTTTCTAGTACGTGCATTCAAATTAAGTTAGGGAAGAATATTAGACAAAAAAAAAAAAAAACATTAACTTTGAAAAATTAGTTCACGTACGAAAAAAAAATTAAAACTTCATAGATTAAAGTTTCCAAATCTTCTAACAACCTGGTTTTCATTGTCCAGGTCACATCCCCACATTCTTTGGGCATCCTCTCAATCTCAGAGCCACCAAGCTTGGGACGGTTCAGGCGAGGGTACGACCGAGCCATCTTCGGTCCAATGCTCCAATTCCTACGAGAAACCAAAGCCCCATTCATGGTGAACCCATATCCCTACTTTGGCTACTCACCAAAAATGGCTAATTACGCCTTATTTCAACCAAATCGTGGCATTTACGATAAGTACACAAAAATCACCTACACCAACATGTTTGATGGCATGATGGACGCCCTCCACTCCGCCATGAAGAAACTCGGCTACGCCGATATTGACCTCGTCATCGGCGAGACCGGTTGGCCCACCAATTGTGACTACGTTGCTTGTAGCCTCGACAATGCTGCTACTTATAATAGGAACTTGATTTGGCATGTCAATTCTGGGAAGGGAACGCCATTGATGCCCAACCGAAAATTTGAGACCTATTTGTTTGGTTTGTTTAATGAGAATCTTAAACCCGGTCCCACAGCCGAGCGGAATTGGGGTTTGTTTCAGCCTGATTTCACTCCGGTTTATGATCTTCCTAGCATCATGCTTGGTGGACAGGTTAGTAATTTTGGTTTAGTCTTTAATCTTCTATTTAATTTTAATTATTTTGATGAGGAATTTTTAAAAATATAAAACTAAGGAAAACTATTTAACGTTGATAGAAGTTTGACATTTTTGTGAAAATTTCTCTATTTTAATTTCTTTGTTTTATAAACTAATTTGTTAGAATGTTTTCAAAATTAAATAAAAGGGTTTGAAAACTAATGTCTATTTGATACTTTAAGCCTTGTTTAGTAACTATTTTGTTTTTTGTTTTTAAAAATTAAGTCTATTTCCTCCTTAATAGTTTACATCTTTAGTATAAGAGTATCATTATTAGCCAAATTCTAAAAACAAAAACTACTTTTTTAAGTTCTCAAAATTTGACTTAGTTTTTTAAAACATTGGTAAATGTAGATAACAAATTAAAAAAATTTAGAAGTAGGAGAGGTAATTCTAAACTTGCTTTTAAAAACTAAAAATTAAAAATCAATTGGTTATTAAAAGGACTTAGTTTTTAACTTTTAAAAATTAAACTCGTAAGTGTTTTTAAAATTAATGTTATAAAAATTTGAGAGTAAAAAACAGTGTCCATAAACTTAATTTTTAAAGAGCACAAAAAAATTAAATGACTATTAACAGGCCCTTTAACTTGGTCATATTTTAACTTTAAAAAAAAACTGGGTCATATTTTGATTTAATACTAATAAATTTAGCTAAAAGCCAATATTAATGACCAATTCCAACAAATTTCCAAACTAAACAGAAAAAAAAGTTAATTACATTAGATTTAAAATAATTTCTTATAATTTATGAACAAAGTAAAATTTCCGTTTCATCATCTCCTACAACAACCCACTAAATCATGTGAGATTTTAAATTACAGCATGGCGGTCGGGGAGGTGGCCGGAGGACGCCGTCGCCGACGCCGGTGGCTCCATTGGGGAAGAAATGGTGCGTTCCGAGATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATTGACTACGTTTGCAGCTCAAACATCGACTGCCGTCCGATCCAAGCCGGCGGCGCGTGTTTTCTACCCAACGACGTCCGATCCCACGCCGCTTACGTCATGAATTCCTATTATCAGACGTCCGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTCTAACCACCGTCAATCCAAGTAATTGCTCCTCTTTTTTTTTTTCTAATTCCGATTAAATTGTTGAATTGATTCGAGATTTTGTGATGTGACCGCCGTGCACGGTGGCTGATTGGCCGTTATCTAATGCAGGTCACAGCGGATGTGCATACGTTTCTTAAGAGATCGAGCGGCGGAGGCCAAGGAAGGATTTTGGTTACGTACGGGCGGGTGCAGGAGTGGAAACCGTGAGGACCACATAAAGCTTACTAATCAATAGAGAGCTGCAAATACTTTTTTTTTTTTTTATTTTTTTATTTTTCTTTCACATAATGATTTATCTTACATTTGTTGTAATTAAATTGGATTCTTCTAAAAAATCTCTCTCTCATTTTACAAAAGTGGGATTTGGAGATTGCAAAGAGATTCCTTTAATTATTCAAACATCATGAGTCCATCCTTCTTTTTCTTTCTTCCCATTTATTGTCAGGGAAAGATTTGAACATTTCGACCGGCTCGATAATTATTTAGTTTTTTAATTTTAAAAATTAAGTCTATAAAGATTTATATTAAATCT

mRNA sequence

AAAAAAGAAGGTGAGGCTGTCATCTTTCGATCTTATCCTCACCCGTGGATATCTTTTTTACTCACACGCCGTCGATTTTCCACCTCAGATTTGATGACCTGGCAATATCCCATTGGTCCGTCTCGTCAAACCTGACACCCACAGGTCACCACCACCACCTTCGCAACACTTTCTCTCTTTCCGTGTTCGCATCGCTATATAAATATTCCCCACTTTCCCCTGTTTCAGAATCCCGTCCCAGTGGTCCTCTCTTCCCTCGCCCCACCCATTTCTCCACCGCCCTCCGACCATGTCCGCCGCCGCATTTCGCCGTCTCTCCGCGGCGCTTTTCCTCCTTCTCCAAATCTCCACCGCCGTCGTCGCCATCGGCGTTAACTACGGCACTCTAGCCAACAACCTCCGCCCGCCGGCGGAGGTGGTGAACTTCCTCAAAACAAAAACAAAAATCGACCGCGTGAAGATCTTCGATACAAATCCGGATATCCTAAGAGCCTTCGCCGGTTCCGGAATCCTGGTGACGGTGACTGTCGGCAACGGCGAGATCCCCGCTCTGGGGGATCTGGCCGCCGCTCGGCAGTGGGTAGCCGCCCATATCGCGCCGTTTCACCCTCAGACGCGGATTAATTACATTGCTGTGGGGAATGAAATCATACACACTAACGATAAGCCTCTGATCGCTCGTCTTGTTCCCGCCATGAGATCGCTCCACCAAGCTCTGGTTCTCGCCGGAATTAAGGACATTAAGGTCACATCCCCACATTCTTTGGGCATCCTCTCAATCTCAGAGCCACCAAGCTTGGGACGGTTCAGGCGAGGGTACGACCGAGCCATCTTCGGTCCAATGCTCCAATTCCTACGAGAAACCAAAGCCCCATTCATGGTGAACCCATATCCCTACTTTGGCTACTCACCAAAAATGGCTAATTACGCCTTATTTCAACCAAATCGTGGCATTTACGATAAGTACACAAAAATCACCTACACCAACATGTTTGATGGCATGATGGACGCCCTCCACTCCGCCATGAAGAAACTCGGCTACGCCGATATTGACCTCGTCATCGGCGAGACCGGTTGGCCCACCAATTGTGACTACGTTGCTTGTAGCCTCGACAATGCTGCTACTTATAATAGGAACTTGATTTGGCATGTCAATTCTGGGAAGGGAACGCCATTGATGCCCAACCGAAAATTTGAGACCTATTTGTTTGGTTTGTTTAATGAGAATCTTAAACCCGGTCCCACAGCCGAGCGGAATTGGGGTTTGTTTCAGCCTGATTTCACTCCGGTTTATGATCTTCCTAGCATCATGCTTGGTGGACAGCATGGCGGTCGGGGAGGTGGCCGGAGGACGCCGTCGCCGACGCCGGTGGCTCCATTGGGGAAGAAATGGTGCGTTCCGAGATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATTGACTACGTTTGCAGCTCAAACATCGACTGCCGTCCGATCCAAGCCGGCGGCGCGTGTTTTCTACCCAACGACGTCCGATCCCACGCCGCTTACGTCATGAATTCCTATTATCAGACGTCCGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTCTAACCACCGTCAATCCAAGTAATTGCTCCTCTTTTTTTTTTTCTAATTCCGATTAAATTGTTGAATTGATTCGAGATTTTGTGATGTGACCGCCGTGCACGGTGGCTGATTGGCCGTTATCTAATGCAGGTCACAGCGGATGTGCATACGTTTCTTAAGAGATCGAGCGGCGGAGGCCAAGGAAGGATTTTGGTTACGTACGGGCGGGTGCAGGAGTGGAAACCGTGAGGACCACATAAAGCTTACTAATCAATAGAGAGCTGCAAATACTTTTTTTTTTTTTTATTTTTTTATTTTTCTTTCACATAATGATTTATCTTACATTTGTTGTAATTAAATTGGATTCTTCTAAAAAATCTCTCTCTCATTTTACAAAAGTGGGATTTGGAGATTGCAAAGAGATTCCTTTAATTATTCAAACATCATGAGTCCATCCTTCTTTTTCTTTCTTCCCATTTATTGTCAGGGAAAGATTTGAACATTTCGACCGGCTCGATAATTATTTAGTTTTTTAATTTTAAAAATTAAGTCTATAAAGATTTATATTAAATCT

Coding sequence (CDS)

ATGTCCGCCGCCGCATTTCGCCGTCTCTCCGCGGCGCTTTTCCTCCTTCTCCAAATCTCCACCGCCGTCGTCGCCATCGGCGTTAACTACGGCACTCTAGCCAACAACCTCCGCCCGCCGGCGGAGGTGGTGAACTTCCTCAAAACAAAAACAAAAATCGACCGCGTGAAGATCTTCGATACAAATCCGGATATCCTAAGAGCCTTCGCCGGTTCCGGAATCCTGGTGACGGTGACTGTCGGCAACGGCGAGATCCCCGCTCTGGGGGATCTGGCCGCCGCTCGGCAGTGGGTAGCCGCCCATATCGCGCCGTTTCACCCTCAGACGCGGATTAATTACATTGCTGTGGGGAATGAAATCATACACACTAACGATAAGCCTCTGATCGCTCGTCTTGTTCCCGCCATGAGATCGCTCCACCAAGCTCTGGTTCTCGCCGGAATTAAGGACATTAAGGTCACATCCCCACATTCTTTGGGCATCCTCTCAATCTCAGAGCCACCAAGCTTGGGACGGTTCAGGCGAGGGTACGACCGAGCCATCTTCGGTCCAATGCTCCAATTCCTACGAGAAACCAAAGCCCCATTCATGGTGAACCCATATCCCTACTTTGGCTACTCACCAAAAATGGCTAATTACGCCTTATTTCAACCAAATCGTGGCATTTACGATAAGTACACAAAAATCACCTACACCAACATGTTTGATGGCATGATGGACGCCCTCCACTCCGCCATGAAGAAACTCGGCTACGCCGATATTGACCTCGTCATCGGCGAGACCGGTTGGCCCACCAATTGTGACTACGTTGCTTGTAGCCTCGACAATGCTGCTACTTATAATAGGAACTTGATTTGGCATGTCAATTCTGGGAAGGGAACGCCATTGATGCCCAACCGAAAATTTGAGACCTATTTGTTTGGTTTGTTTAATGAGAATCTTAAACCCGGTCCCACAGCCGAGCGGAATTGGGGTTTGTTTCAGCCTGATTTCACTCCGGTTTATGATCTTCCTAGCATCATGCTTGGTGGACAGCATGGCGGTCGGGGAGGTGGCCGGAGGACGCCGTCGCCGACGCCGGTGGCTCCATTGGGGAAGAAATGGTGCGTTCCGAGATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATTGACTACGTTTGCAGCTCAAACATCGACTGCCGTCCGATCCAAGCCGGCGGCGCGTGTTTTCTACCCAACGACGTCCGATCCCACGCCGCTTACGTCATGAATTCCTATTATCAGACGTCCGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTCTAACCACCGTCAATCCAAGTAATTGCTCCTCTTTTTTTTTTTCTAATTCCGATTAA

Protein sequence

MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRAIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMDALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPVAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFSNSD

Homology

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BLAST of Lsi04G008770 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 3.4e-125
Identity = 224/435 (51.49%), Postives = 292/435 (67.13%), Query Frame = 0


Query: 20  STAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAGSGILVTVT 79
           +T  + IGVNYG  A+NL  P  V  FL TKT IDRVK+FD NP  + AFAG+ I + V+
Sbjct: 20  ATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVS 79

Query: 80  VGNGEIPALGD----LAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIARLVPA 139
           + N  +PAL D    L AAR W+ A+++P+ P T +  +  GNEI+ + D  LI  L+PA
Sbjct: 80  LPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPA 139

Query: 140 MRSLHQALVLAGIKDIKVTSPHSLGILSISEP-PSLGRFRRGYDRAIFGPMLQFLRETKA 199
           MR L QAL   G+  ++VT+PH LGIL+ S+  PS   FR GY+  +F  MLQF R+T +
Sbjct: 140 MRRLAQALKAEGLTGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGS 199

Query: 200 PFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMDALHSAMKKLGYADI 259
           PFMVNPYPYF Y P+  NYALF+PN GIYD  TK+ YT+M D  MDA+++AMKKLGY D+
Sbjct: 200 PFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDV 259

Query: 260 DLVIGETGWPTNCD--YVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNE 319
           D+ +GE GWPT  +   +   +  A  +N  +I   +SGKGTPLMPNR FETYLF LF+E
Sbjct: 260 DIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDE 319

Query: 320 NLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTP-VAPL-GKKWCV 379
           N KPGP AER++GLF PDFTPVYDL  +  G           +P+P+P  AP  G KWCV
Sbjct: 320 NQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPSPNPSPKPAPSGGGKWCV 379

Query: 380 PRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFN 439
            +  A    LQ NI+Y C   +DC+PIQ+GGACF PN +++HA+YVMN+YYQ +G  D  
Sbjct: 380 AKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLA 439

Query: 440 CDFGHTGVLTTVNPS 446
           CDF  TG++T+ +PS
Sbjct: 440 CDFKGTGIVTSSDPS 453

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BLAST of Lsi04G008770 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 374.8 bits (961), Expect = 1.4e-102
Identity = 198/459 (43.14%), Postives = 286/459 (62.31%), Query Frame = 0


Query: 9   LSAALFLLL------QISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTN 68
           LS +++ LL        S A   IGVNYG +A+NL PP+E V  L++ T I +V+++  +
Sbjct: 3   LSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGAD 62

Query: 69  PDILRAFAGSGILVTVTVGNGEIPAL-GDLAAARQWVAAHIAPFHPQTRINYIAVGNEII 128
           P I++A AG+G+ + +   NG++P+L  D  AA QW+ +++ PF+P ++I  I VGNEI+
Sbjct: 63  PAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEIL 122

Query: 129 HTNDKPLIARLVPAMRSLHQALVLAGI-KDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 188
            +ND  L+ +L+PAM+++ +AL    +   IKV++ +S+ +L  S+PPS G F  GY   
Sbjct: 123 MSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG 182

Query: 189 IFGPMLQFLRETKAPFMVNPYPYFGYS----PKMANYALFQPNRGIYDKYTKITYTNMFD 248
           + G +LQFL +T +PF +NPYP+F Y     P+   + LF+PN G  D  T I YTNMFD
Sbjct: 183 LKG-ILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFD 242

Query: 249 GMMDALHSAMKKLGYADIDLVIGETGWPTNCD--YVACSLDNAATYNRNLIWHVNSGKGT 308
             +DA+HSA+K +G+  +++V+ ETGW +  D   V  S+DNA  YN NLI H+ S  GT
Sbjct: 243 AQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGT 302

Query: 309 PLMPNRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRR 368
           PLMP +  +TY+F L++ENLKPGP++ER +GLF+ D + VYD+              G  
Sbjct: 303 PLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDV--------------GLA 362

Query: 369 TPSPTPVAPLGK----KWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVR 428
             S +   P GK     WCVP+  A  E LQ ++D+ C   IDC  IQ GGACF PN+V 
Sbjct: 363 KSSSSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVV 422

Query: 429 SHAAYVMNSYYQTSGRHDFNCDFGHTGVLTTVNPS--NC 448
           SHAAY MN Y+Q S +   +CDF  T  +T+ NPS  NC
Sbjct: 423 SHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNC 445

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BLAST of Lsi04G008770 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 8.8e-97
Identity = 195/455 (42.86%), Postives = 280/455 (61.54%), Query Frame = 0


Query: 13  LFLLLQ---ISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAF 72
           +FLLLQ    + +   +GVNYG L++NL      VN LK+ T I +V++F   P +++AF
Sbjct: 14  VFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKS-TTIQKVRLFGAEPAVIKAF 73

Query: 73  AGSGILVTVTVGNGEIPAL-GDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPL 132
           A +G+ + +   NG+IP L  +   A Q+V +++  F+P + I  I VGNE++ + D+ L
Sbjct: 74  ANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKL 133

Query: 133 IARLVPAMRSLHQALVLAGI-KDIKVTSPHSLGILSISEPPSLGRFRRGYDRAIFGPMLQ 192
           I++L+PAM+++  AL  A +   +KV++ H++ +LS S PPS G F  G    +   +LQ
Sbjct: 134 ISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTM-KALLQ 193

Query: 193 FLRETKAPFMVNPYPYFGY----SPKMANYALFQPNRGIYDKYTKITYTNMFDGMMDALH 252
           F     APFM++PYPYF Y    +P    + LFQPN G  D      YTNMFD  +DA+H
Sbjct: 194 FQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVH 253

Query: 253 SAMKKLGYADIDLVIGETGWPTNCD--YVACSLDNAATYNRNLIWHVNSGKGTPLMPNRK 312
           SA+  +G+ DI++V+ ETGWP   D   V  SLDNA  Y  NLI H+ S  GTPLMP + 
Sbjct: 254 SALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKS 313

Query: 313 FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPV 372
            +TYLF L++E+ K G ++E+ +GLF+PD +  YD+  ++   Q+        TP+PTP 
Sbjct: 314 IDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDV-GLLKNTQNPTTPA---TPTPTPK 373

Query: 373 APLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYY 432
           A     WCVP+   + + L  NI+Y C   IDC PIQ GGACF PN V++HAAYVMN YY
Sbjct: 374 A--AGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYY 433

Query: 433 QTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFSNSD 457
           Q++GR+ +NCDF  T  LT  NPS  +  F S S+
Sbjct: 434 QSAGRNSWNCDFSQTATLTNTNPSYGACNFPSGSN 460

[Close]

BLAST of Lsi04G008770 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.8e-76
Identity = 165/450 (36.67%), Postives = 245/450 (54.44%), Query Frame = 0


Query: 26  IGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAGSGILVTVTVGNGEI 85
           IG+ YG  A+NL  P  V   ++    I  V+I+D N D+L+AFA +GI + + V N ++
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQ-HLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADL 86

Query: 86  PALGDLAA-ARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIARLVPAMRSLHQALV 145
            A     +    W++ +I P++P T+I  I+VG E+    D      ++PAMR++H AL 
Sbjct: 87  LAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-ATGLVLPAMRNIHTALK 146

Query: 146 LAGI-KDIKVTSPHSLGILSISEPPSLGRFRRGYDRAIFGPMLQFLRETKAPFMVNPYPY 205
            +G+ K IK++S HSL ILS S PPS   F + +  A   PML+FL E ++PFM++ YPY
Sbjct: 147 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLKPMLEFLVENESPFMIDLYPY 206

Query: 206 FGYSPKM----ANYALFQPNRGIYDKYTKITYTNMFDGMMDALHSAMKKLGYADIDLVIG 265
           + Y          YALF+ +  + D  T + Y+NMFD  +DA++ A+  + +  + +++ 
Sbjct: 207 YAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 266 ETGWPT--NCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNENLKPG 325
           E+GWP+  +    A + +NA  YN NLI HV    GTP  P  + + YLF LFNEN KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 326 PTAERNWGLFQPDFTPVYDL----PSIMLGGQHGGRGGGRRTPSPTPV------APLG-- 385
             +ERNWG+F  + T VY L     +           G   +PS +P+        +G  
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGG 386

Query: 386 -----KKWCVPRSDAAIEALQKNIDYVCS-SNIDCRPIQAGGACFLPNDVRSHAAYVMNS 445
                KKWC+  S A++  LQ  +D+ C   N+DC  +Q    CF P+ V SHA+Y  N+
Sbjct: 387 GGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNT 446

Query: 446 YYQTSGRHDFNCDFGHTGVLTTVNPS--NC 448
           YYQ SG    +C F    V    +PS  NC
Sbjct: 447 YYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

[Close]

BLAST of Lsi04G008770 vs. ExPASy Swiss-Prot
Match: Q06915 (Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=A6 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 8.6e-76
Identity = 165/432 (38.19%), Postives = 235/432 (54.40%), Query Frame = 0


Query: 26  IGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAGSGILVTVTVGNGEI 85
           IG+NYG   NNL  P + +NF+K+  K   VK++D +P+ L   + + + VT+TV N +I
Sbjct: 42  IGINYGRRGNNLPSPYQSINFIKS-IKAGHVKLYDADPESLTLLSQTNLYVTITVPNHQI 101

Query: 86  PAL-GDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIARLVPAMRSLHQALV 145
            AL  +   A +WV  +I P++PQT+I ++ VGNEI+  N   +   LVPAMR +  +L 
Sbjct: 102 TALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIVNSLR 161

Query: 146 LAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRAIFGPMLQFLRETKAPFMVNPYPYF 205
           L GI +IKV +P ++  L  S PPS G FR      +  P+L+FL  T + F +N +PYF
Sbjct: 162 LHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNVHPYF 221

Query: 206 GYSPKMAN----YALFQPNRGIYDKYTKITYTNMFDGMMDALHSAMKKLGYADIDLVIGE 265
            +S    N    +ALFQ +    D  T + Y N+ D M+D++  AM KLGY  + L I E
Sbjct: 222 RWSRNPMNTSLDFALFQGHSTYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMRLAISE 281

Query: 266 TGWPT--NCDYVACSLDNAATYNRNLIWHVNSGK--GTPLMPNRKFETYLFGLFNENLKP 325
           TGWP   + D    ++ NAATYNRNLI  +++    GTP  P     T++F LFNEN K 
Sbjct: 282 TGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFNENQKS 341

Query: 326 GPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPVAPL-GKKWCVPRSDA 385
           G   +R+WG+  PD +P+YD   +   GQ     G    P PT   P  G+ WCVP   A
Sbjct: 342 GSGTQRHWGILHPDGSPIYD---VDFTGQ-TPLTGFNPLPKPTNNVPYKGQVWCVPVEGA 401

Query: 386 AIEALQKNIDYVCS-SNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNCDFG 445
               L++ +   C+ SN  C  +  G  C+ P  +  HA+Y +NSY+         C F 
Sbjct: 402 NETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFFN 461

Query: 446 HTGVLTTVNPSN 447
                TT NP N
Sbjct: 462 GLAHETTTNPGN 468

[Close]

BLAST of Lsi04G008770 vs. ExPASy TrEMBL
Match: A0A0A0KS71 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.1e-238
Identity = 402/446 (90.13%), Postives = 424/446 (95.07%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQIS-TAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIF 60
           MSAAA RRLSAALFLLLQIS TAV AIGVNYGTL NNLRPPAEV NFLKTKTKIDRVKIF
Sbjct: 1   MSAAALRRLSAALFLLLQISTTAVFAIGVNYGTLGNNLRPPAEVANFLKTKTKIDRVKIF 60

Query: 61  DTNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNE 120
           DTNPDILRAFAGSGILVTVTVGNG IP L DLA AR+WVAAHIAPFHPQTRI+YIAVGNE
Sbjct: 61  DTNPDILRAFAGSGILVTVTVGNGLIPNLADLAFAREWVAAHIAPFHPQTRIHYIAVGNE 120

Query: 121 IIHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDR 180
           IIH+NDKPLIARLVPAMRSLH+ALVLAGI+D+KVTSPHSLGILSISEPPS+GRFRRGYDR
Sbjct: 121 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180

Query: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMM 240
           AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDKYTKITYTNMFD MM
Sbjct: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTKITYTNMFDSMM 240

Query: 241 DALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPN 300
           DA++SAMKKLGYAD+++V+GETGWPTNCDY+ACS DNAATYNRNL+WHVNSGKGTPLMPN
Sbjct: 241 DAIYSAMKKLGYADVNIVLGETGWPTNCDYIACSPDNAATYNRNLVWHVNSGKGTPLMPN 300

Query: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPT 360
           RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY LP+IMLGG+  GRGG R    PT
Sbjct: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYSLPNIMLGGR--GRGGRR----PT 360

Query: 361 PVAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNS 420
           PVAP GKKWCVPRSDAA++ALQKNIDYVCSSN+DCRPIQAGGACFLPNDVRSHAAYVMNS
Sbjct: 361 PVAPSGKKWCVPRSDAAVDALQKNIDYVCSSNVDCRPIQAGGACFLPNDVRSHAAYVMNS 420

Query: 421 YYQTSGRHDFNCDFGHTGVLTTVNPS 446
           YYQTSGRHD+NCDF HTGVLTTVNPS
Sbjct: 421 YYQTSGRHDYNCDFSHTGVLTTVNPS 440

[Close]

BLAST of Lsi04G008770 vs. ExPASy TrEMBL
Match: A0A5D3BZM0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00090 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.5e-236
Identity = 398/453 (87.86%), Postives = 421/453 (92.94%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFD 60
           MSAA   RLSAA+FLLLQIST V AIGVNYGTL NNLRPPAEVVNFLKTKTKIDRVKIFD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIPALG+LA AR WVA HIAPFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 180
           IH+NDKPLIARLVPAMRSLH+ALVLAGI DIKVTSPHSLGILSISEPPS+GRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDKYT+ITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 ALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNR 300
           A++SAMKKLGYADI +VIGETGWPTNCDY+ACS DNAAT+NRNL+WHVNSGKGTPLMPNR
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCDYIACSPDNAATFNRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTP 360
           KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY+LP+IML    GGRG   R PSPTP
Sbjct: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIML----GGRGRRGRRPSPTP 360

Query: 361 VAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSY 420
           +AP  KKWCVPRS AA+EALQKNIDYVCSS +DCRPIQAGGACFLPNDVRSHAAYVMN+Y
Sbjct: 361 IAPSAKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAY 420

Query: 421 YQTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFS 454
           YQTSGRHD+NCDFG TGVLTTVNPS+    + S
Sbjct: 421 YQTSGRHDYNCDFGQTGVLTTVNPSHGGCVYVS 449

[Close]

BLAST of Lsi04G008770 vs. ExPASy TrEMBL
Match: A0A1S3CF05 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.5e-236
Identity = 398/453 (87.86%), Postives = 421/453 (92.94%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFD 60
           MSAA   RLSAA+FLLLQIST V AIGVNYGTL NNLRPPAEVVNFLKTKTKIDRVKIFD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIPALG+LA AR WVA HIAPFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 180
           IH+NDKPLIARLVPAMRSLH+ALVLAGI DIKVTSPHSLGILSISEPPS+GRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDKYT+ITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 ALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNR 300
           A++SAMKKLGYADI +VIGETGWPTNCDY+ACS DNAAT+NRNL+WHVNSGKGTPLMPNR
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCDYIACSPDNAATFNRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTP 360
           KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY+LP+IML    GGRG   R PSPTP
Sbjct: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIML----GGRGRRGRRPSPTP 360

Query: 361 VAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSY 420
           +AP  KKWCVPRS AA+EALQKNIDYVCSS +DCRPIQAGGACFLPNDVRSHAAYVMN+Y
Sbjct: 361 IAPSAKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAY 420

Query: 421 YQTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFS 454
           YQTSGRHD+NCDFG TGVLTTVNPS+    + S
Sbjct: 421 YQTSGRHDYNCDFGQTGVLTTVNPSHGGCVYVS 449

[Close]

BLAST of Lsi04G008770 vs. ExPASy TrEMBL
Match: A0A6J1GZM3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 PE=3 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 6.7e-233
Identity = 390/454 (85.90%), Postives = 419/454 (92.29%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTL NNLRPPAEVVNF+KTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIP L DLA ARQWVAAHI PFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+ALVLAGI DIKVTSPHSL ILSISEPPS+GRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMD 240
           IF PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDKYTKITYTNMFD MMD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNR 300
           AL+SAMK +GY+D+DLVIGETGWPTNCDYVACS +NAAT++RNL+WHVNSGKGTPLMPNR
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCDYVACSPNNAATFHRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQH-GGRGGGRRTPSPT 360
           KFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GG+H GGRGGG     P 
Sbjct: 301 KFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGG-----PK 360

Query: 361 PVAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNS 420
           PVAP GKKWCVP+SDA+++ALQ NIDYVCSSNIDCRPIQAGGACFLPND+RSHA+Y MNS
Sbjct: 361 PVAPSGKKWCVPKSDASVQALQNNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNS 420

Query: 421 YYQTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFS 454
           YYQTSGR+DFNCDF HTGV+TTVNPS+    + S
Sbjct: 421 YYQTSGRNDFNCDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Lsi04G008770 vs. ExPASy TrEMBL
Match: A0A6J1JYX6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.7e-231
Identity = 389/454 (85.68%), Postives = 419/454 (92.29%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTLANNLRPPAEVVNF+KTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
           TNPDILRAFAG+GILVTVTVGNGEIPAL DLA ARQWVAAHI PFHPQTRINYIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPALTDLATARQWVAAHIRPFHPQTRINYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+ALVLAGI DIKVTSPHSL ILSISEPPS+GRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDKYTKITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKKLGYADIDLVIGETGWPTNCD-YVACSLDNAATYNRNLIWHVNSGKGTPLMPN 300
           AL+SAMK +GY+D+DLVIGETGWP+NCD YVACS  NAAT++RNL+WHVNSGKGTPLMPN
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPSNCDGYVACSPGNAATFHRNLVWHVNSGKGTPLMPN 300

Query: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPT 360
           RKFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GG+H G  GGR    P 
Sbjct: 301 RKFETYLFALFNENLKPGPTAERNWGLFHPDFTPVYDVPNIMIGGRHRGGRGGR----PK 360

Query: 361 PVAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNS 420
           PVAP GKKWCVP+SDA+++ALQKNIDYVCSSNIDCRPIQAGGACFLPND+R+HA+Y MNS
Sbjct: 361 PVAPSGKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRAHASYAMNS 420

Query: 421 YYQTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFS 454
           YYQTSGR+DFNCDF  TGV+TTVNPS+    + S
Sbjct: 421 YYQTSGRNDFNCDFSLTGVITTVNPSHGGCVYIS 450

[Close]

BLAST of Lsi04G008770 vs. NCBI nr
Match: XP_038891662.1 (glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida])

HSP 1 Score: 880.2 bits (2273), Expect = 7.8e-252
Identity = 420/445 (94.38%), Postives = 437/445 (98.20%), Query Frame = 0


Query: 2   SAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDT 61
           SAAAF R+SAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDT
Sbjct: 57  SAAAFHRISAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDT 116

Query: 62  NPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEII 121
           NPDILRAFAGSGILVTVTVGNGEIPALGDLA ARQWVAAHIAPFHP+TRI+YIAVGNEII
Sbjct: 117 NPDILRAFAGSGILVTVTVGNGEIPALGDLAVARQWVAAHIAPFHPRTRIHYIAVGNEII 176

Query: 122 HTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRAI 181
           H+NDKPLIARLVPAMRSLH+ALVLAGI+D+KVTSPHSLGILSISEPPS+GRFRRGYDR I
Sbjct: 177 HSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDRTI 236

Query: 182 FGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMDA 241
           FGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALF+PNRGIYDKYTKITYTNMFDGMMDA
Sbjct: 237 FGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFRPNRGIYDKYTKITYTNMFDGMMDA 296

Query: 242 LHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRK 301
           L+SAMKKLGYAD+DLVIGETGWPTNCDY+ACS DNAATYNRNLIWHVNSGKGTPLMPNRK
Sbjct: 297 LYSAMKKLGYADVDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRK 356

Query: 302 FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPV 361
           FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLP+IMLG + GGRGGGRRTPSPTPV
Sbjct: 357 FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGRRRGGRGGGRRTPSPTPV 416

Query: 362 APLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYY 421
           AP G+KWCVPRSDA IEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYY
Sbjct: 417 APSGQKWCVPRSDAPIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYY 476

Query: 422 QTSGRHDFNCDFGHTGVLTTVNPSN 447
           QT+GRHDFNCDFGHTGVLTTVNPS+
Sbjct: 477 QTAGRHDFNCDFGHTGVLTTVNPSH 501

[Close]

BLAST of Lsi04G008770 vs. NCBI nr
Match: XP_004147695.2 (glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN50054.2 hypothetical protein Csa_021427 [Cucumis sativus])

HSP 1 Score: 833.6 bits (2152), Expect = 8.4e-238
Identity = 402/447 (89.93%), Postives = 425/447 (95.08%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQIS-TAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIF 60
           MSAAA RRLSAALFLLLQIS TAV AIGVNYGTL NNLRPPAEV NFLKTKTKIDRVKIF
Sbjct: 1   MSAAALRRLSAALFLLLQISTTAVFAIGVNYGTLGNNLRPPAEVANFLKTKTKIDRVKIF 60

Query: 61  DTNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNE 120
           DTNPDILRAFAGSGILVTVTVGNG IP L DLA AR+WVAAHIAPFHPQTRI+YIAVGNE
Sbjct: 61  DTNPDILRAFAGSGILVTVTVGNGLIPNLADLAFAREWVAAHIAPFHPQTRIHYIAVGNE 120

Query: 121 IIHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDR 180
           IIH+NDKPLIARLVPAMRSLH+ALVLAGI+D+KVTSPHSLGILSISEPPS+GRFRRGYDR
Sbjct: 121 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180

Query: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMM 240
           AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDKYTKITYTNMFD MM
Sbjct: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTKITYTNMFDSMM 240

Query: 241 DALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPN 300
           DA++SAMKKLGYAD+++V+GETGWPTNCDY+ACS DNAATYNRNL+WHVNSGKGTPLMPN
Sbjct: 241 DAIYSAMKKLGYADVNIVLGETGWPTNCDYIACSPDNAATYNRNLVWHVNSGKGTPLMPN 300

Query: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPT 360
           RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY LP+IMLGG+  GRGG R    PT
Sbjct: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYSLPNIMLGGR--GRGGRR----PT 360

Query: 361 PVAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNS 420
           PVAP GKKWCVPRSDAA++ALQKNIDYVCSSN+DCRPIQAGGACFLPNDVRSHAAYVMNS
Sbjct: 361 PVAPSGKKWCVPRSDAAVDALQKNIDYVCSSNVDCRPIQAGGACFLPNDVRSHAAYVMNS 420

Query: 421 YYQTSGRHDFNCDFGHTGVLTTVNPSN 447
           YYQTSGRHD+NCDF HTGVLTTVNPS+
Sbjct: 421 YYQTSGRHDYNCDFSHTGVLTTVNPSH 441

[Close]

BLAST of Lsi04G008770 vs. NCBI nr
Match: XP_008461659.1 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo] >KAA0050300.1 glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK03519.1 glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 825.9 bits (2132), Expect = 1.8e-235
Identity = 398/453 (87.86%), Postives = 421/453 (92.94%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFD 60
           MSAA   RLSAA+FLLLQIST V AIGVNYGTL NNLRPPAEVVNFLKTKTKIDRVKIFD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIPALG+LA AR WVA HIAPFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 180
           IH+NDKPLIARLVPAMRSLH+ALVLAGI DIKVTSPHSLGILSISEPPS+GRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDKYT+ITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 ALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNR 300
           A++SAMKKLGYADI +VIGETGWPTNCDY+ACS DNAAT+NRNL+WHVNSGKGTPLMPNR
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCDYIACSPDNAATFNRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTP 360
           KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY+LP+IML    GGRG   R PSPTP
Sbjct: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIML----GGRGRRGRRPSPTP 360

Query: 361 VAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSY 420
           +AP  KKWCVPRS AA+EALQKNIDYVCSS +DCRPIQAGGACFLPNDVRSHAAYVMN+Y
Sbjct: 361 IAPSAKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAY 420

Query: 421 YQTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFS 454
           YQTSGRHD+NCDFG TGVLTTVNPS+    + S
Sbjct: 421 YQTSGRHDYNCDFGQTGVLTTVNPSHGGCVYVS 449

[Close]

BLAST of Lsi04G008770 vs. NCBI nr
Match: KAG7031616.1 (Glucan endo-1,3-beta-glucosidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 817.8 bits (2111), Expect = 4.8e-233
Identity = 390/446 (87.44%), Postives = 417/446 (93.50%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTL NNLRPPAEVVNF+KTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLLAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
           TNPDILRAFAG+GILVTVTVGNGEIP L DLA ARQWVAAHI PFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+ALVLAGI DIKVTSPHSL ILSISEPPSLGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSLGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMD 240
           IF PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDKYTKITYTNMFD MMD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNR 300
           AL+SAMK +GY+D+DLVIGETGWPTNCDYVACS +NAAT++RNL+WHVNSGKGTPLMPNR
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCDYVACSPNNAATFHRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQH-GGRGGGRRTPSPT 360
           KFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GG+H GGRGGG     P 
Sbjct: 301 KFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGG-----PK 360

Query: 361 PVAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNS 420
           PVAP GKKWCVP+SDA+++ALQKNIDYVCSSNIDCRPIQAGGACFLPND+RSHA+Y MNS
Sbjct: 361 PVAPSGKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNS 420

Query: 421 YYQTSGRHDFNCDFGHTGVLTTVNPS 446
           YYQTSGR+DFNCDF HTGV+TTVNPS
Sbjct: 421 YYQTSGRNDFNCDFSHTGVITTVNPS 441

[Close]

BLAST of Lsi04G008770 vs. NCBI nr
Match: KAG6601002.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 817.8 bits (2111), Expect = 4.8e-233
Identity = 391/454 (86.12%), Postives = 420/454 (92.51%), Query Frame = 0


Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTL NNLRPPAEVVNF+KTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
           TNPDILRAFAG+GILVTVTVGNGEIP L DLA ARQWVAAHI PFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+ALVLAGI DIKVTSPHSL ILSISEPPSLGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSLGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMD 240
           IF PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDKYTKITYTNMFD MMD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNR 300
           AL+SAMK +GY+D+DLVIGETGWPTNCDYVACS +NAAT++RNL+WHVNSGKGTPLMPNR
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCDYVACSPNNAATFHRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQH-GGRGGGRRTPSPT 360
           KFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GG+H GGRGGG     P 
Sbjct: 301 KFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGG-----PK 360

Query: 361 PVAPLGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNS 420
           PVAP GKKWCVP+SDA+++ALQKNIDYVCSSNIDCRPIQAGGACFLPND+RSHA+Y MNS
Sbjct: 361 PVAPSGKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNS 420

Query: 421 YYQTSGRHDFNCDFGHTGVLTTVNPSNCSSFFFS 454
           YYQTSGR+DFNCDF HTGV+TTVNPS+    + S
Sbjct: 421 YYQTSGRNDFNCDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Lsi04G008770 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 507.7 bits (1306), Expect = 9.8e-144
Identity = 250/441 (56.69%), Postives = 324/441 (73.47%), Query Frame = 0


Query: 9   LSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRA 68
           L AA+F  L I   + AIGVNYGTL  NL PP +V NF+KT+T ID VKIFD NPDILRA
Sbjct: 14  LCAAVF--LTIPAVISAIGVNYGTL-GNLPPPTQVANFIKTQTSIDSVKIFDVNPDILRA 73

Query: 69  FAGSGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPL 128
           FAG+GI V VTV NG+IPAL +   AR+WV+ +I PFHPQT+I YI+VGNEI+ T D  +
Sbjct: 74  FAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLTGDNNM 133

Query: 129 IARLVPAMRSLHQALVLAGIKDIKVTSPHSLGILS--ISEPPSLGRFRRGYDRAIFGPML 188
           I  L+PAMR+L+ ALV AG++D+KVT+ HSL I++  ++  PS GRFR G+D+ I  P+L
Sbjct: 134 INNLLPAMRNLNNALVRAGVRDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKGILAPIL 193

Query: 189 QFLRETKAPFMVNPYPYFGYSPKMANYALFQ-PNRGIYDKYTKITYTNMFDGMMDALHSA 248
            + R TK+PFMVNPYPYFG+ PK  N+A+F+ P + + D +T+  YTNMFD +MD+ +SA
Sbjct: 194 AYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALMDSTYSA 253

Query: 249 MKKLGYADIDLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETY 308
           MK LGY D+++V+GETGWP+ CD   CS  NAA +N N+I     G+GTPLMPNR+FETY
Sbjct: 254 MKALGYGDVNIVVGETGWPSACDAPWCSPANAAWFNLNIIKRA-QGQGTPLMPNRRFETY 313

Query: 309 LFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPVAPLG 368
           +FGLFNE  KPGPTAERNWGLF+ DF+PVYD     +G    G+GGG R   P P +  G
Sbjct: 314 IFGLFNEEGKPGPTAERNWGLFRADFSPVYD-----VGLLRNGQGGGGRPALPAP-STAG 373

Query: 369 KKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSG 428
            KWCV RS A    LQ +I++VC   +DC+PIQAGG+CF P+ +R+HA++VMN+Y+Q+ G
Sbjct: 374 GKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLRTHASFVMNAYFQSHG 433

Query: 429 RHDFNCDFGHTGVLTTVNPSN 447
           R D  C+F  TG++   NPSN
Sbjct: 434 RTDGACNFSGTGMIVGNNPSN 444

[Close]

BLAST of Lsi04G008770 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.3e-135
Identity = 229/438 (52.28%), Postives = 315/438 (71.92%), Query Frame = 0


Query: 15  LLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAGSGI 74
           LL+  + +  +IGVNYGTLANNL PP +V  FL   T I+R+++FDT+P IL+AFA +GI
Sbjct: 19  LLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFANTGI 78

Query: 75  LVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIARLVP 134
            VTVTV N +IP L +L++A+QW++ HI P  P T I  I VGNE+I T D  LI  L+P
Sbjct: 79  AVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRTLIP 138

Query: 135 AMRSLHQALVLAGI-KDIKVTSPHSLGILSISEPPSLGRFRRGYDRAIFGPMLQFLRETK 194
           AM+SLH ALV A + + I++++PHSLG L+ S PPS  +FRRGYD  +  P+L FLR T 
Sbjct: 139 AMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLRSTS 198

Query: 195 APFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMDALHSAMKKLGYAD 254
           +PF+VNPYP+FGYS +  ++ALF+PN G++D++TK+ YTNM D  +D+++SAM KLG++D
Sbjct: 199 SPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLGFSD 258

Query: 255 IDLVIGETGWPT--NCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFN 314
           +++VIGE GWP+  + D +   +D AA +N+NLI  V+SG GTPLMPNR FETY+F LFN
Sbjct: 259 VEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFALFN 318

Query: 315 ENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPVAPLGKKWCVP 374
           ENLK GPT+ERN+G+F+ D TP+YD+  +    +        R+P         K+WCV 
Sbjct: 319 ENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR---GSSSKRWCVT 378

Query: 375 RSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNC 434
           ++ A   ALQ+NIDYVC   +DCRPI  GG C+LPN V++H+ Y MN YYQT  +H+F+C
Sbjct: 379 KAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQTMEKHEFDC 438

Query: 435 DFGHTGVLTTVNPS--NC 448
           DF +TG +TT++PS  NC
Sbjct: 439 DFDNTGEITTIDPSYGNC 453

[Close]

BLAST of Lsi04G008770 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 480.3 bits (1235), Expect = 1.7e-135
Identity = 227/434 (52.30%), Postives = 313/434 (72.12%), Query Frame = 0


Query: 15  LLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAGSGI 74
           LL+  + +  +IGVNYGTLANNL PP +V  FL   T I+R+++FDT+P IL+AFA +GI
Sbjct: 19  LLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFANTGI 78

Query: 75  LVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIARLVP 134
            VTVTV N +IP L +L++A+QW++ HI P  P T I  I VGNE+I T D  LI  L+P
Sbjct: 79  AVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRTLIP 138

Query: 135 AMRSLHQALVLAGI-KDIKVTSPHSLGILSISEPPSLGRFRRGYDRAIFGPMLQFLRETK 194
           AM+SLH ALV A + + I++++PHSLG L+ S PPS  +FRRGYD  +  P+L FLR T 
Sbjct: 139 AMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLRSTS 198

Query: 195 APFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMDALHSAMKKLGYAD 254
           +PF+VNPYP+FGYS +  ++ALF+PN G++D++TK+ YTNM D  +D+++SAM KLG++D
Sbjct: 199 SPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLGFSD 258

Query: 255 IDLVIGETGWPT--NCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFN 314
           +++VIGE GWP+  + D +   +D AA +N+NLI  V+SG GTPLMPNR FETY+F LFN
Sbjct: 259 VEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFALFN 318

Query: 315 ENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPVAPLGKKWCVP 374
           ENLK GPT+ERN+G+F+ D TP+YD+  +    +        R+P         K+WCV 
Sbjct: 319 ENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR---GSSSKRWCVT 378

Query: 375 RSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNC 434
           ++ A   ALQ+NIDYVC   +DCRPI  GG C+LPN V++H+ Y MN YYQT  +H+F+C
Sbjct: 379 KAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQTMEKHEFDC 438

Query: 435 DFGHTGVLTTVNPS 446
           DF +TG +TT++PS
Sbjct: 439 DFDNTGEITTIDPS 449

[Close]

BLAST of Lsi04G008770 vs. TAIR 10
Match: AT2G39640.1 (glycosyl hydrolase family 17 protein )

HSP 1 Score: 439.9 bits (1130), Expect = 2.5e-123
Identity = 218/433 (50.35%), Postives = 290/433 (66.97%), Query Frame = 0


Query: 15  LLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAGSGI 74
           ++ Q+S    AIG+NYGTL  NL+PP +VV+F+KTKT  D VKI+D NPDILRAFAGS I
Sbjct: 19  VIFQLSAVTSAIGINYGTL-GNLQPPQQVVDFIKTKTTFDSVKIYDANPDILRAFAGSEI 78

Query: 75  LVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIARLVP 134
            +T+ V NG IPA+ ++A ARQWVAA++ PF  Q +  Y+ VGNEI+ +ND  LI+ LVP
Sbjct: 79  NITIMVPNGNIPAMVNVANARQWVAANVLPFQQQIKFKYVCVGNEILASNDNNLISNLVP 138

Query: 135 AMRSLHQALVLAGIKDIKVTSPHSLGILSISEPPSLGRFRRGYDRAIFGPMLQFLRETKA 194
           AM+SL++AL  + +  IKVT+PH+  I      PS  RF     + IF  +L+F R+ K+
Sbjct: 139 AMQSLNEALKASNLTYIKVTTPHAFTISYNRNTPSESRFTND-QKDIFTKILEFHRQAKS 198

Query: 195 PFMVNPYPYFGYSPKMANYALFQPNRGIYDKYTKITYTNMFDGMMDALHSAMKKLGYADI 254
           PFM+N Y +F       NYA+F P+  I D  T+ TYTNMFD +MDA +SAMK LGY D+
Sbjct: 199 PFMINAYTFFTMDTNNVNYAIFGPSNAITDTNTQQTYTNMFDAVMDATYSAMKALGYGDV 258

Query: 255 DLVIGETGWPTNCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNENL 314
           D+ +GETGWPT CD   CS  NA  YN N+I       GTPLMPNR  + ++F LFNE+ 
Sbjct: 259 DIAVGETGWPTACDASWCSPQNAENYNLNIIKRAQV-IGTPLMPNRHIDIFIFALFNEDG 318

Query: 315 KPGPTAERNWGLFQPDFTPVYDLPSIMLGGQHGGRGGGRRTPSPTPVAPLGK--KWCVPR 374
           KPGPT ERNWG+F+PDF+P+YD+  +        +GGG    SP P  P+    KWCV +
Sbjct: 319 KPGPTRERNWGIFKPDFSPMYDVGVL--------KGGG----SPLPFPPINNNGKWCVGK 378

Query: 375 SDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNCD 434
            +A +  LQ NID+VCS  IDC PI  GG CF  N++ + ++++MN+YYQ+ G  D  CD
Sbjct: 379 PEATLMQLQANIDWVCSHGIDCTPISPGGICFDNNNMTTRSSFIMNAYYQSKGCVDVVCD 436

Query: 435 FGHTGVLTTVNPS 446
           F  TG++T+ NPS
Sbjct: 439 FSGTGIVTSTNPS 436

[Close]

BLAST of Lsi04G008770 vs. TAIR 10
Match: AT3G46570.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 3.3e-107
Identity = 198/330 (60.00%), Postives = 246/330 (74.55%), Query Frame = 0


Query: 13  LFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAFAG- 72
           L L+L  S A   +GVNYGT+ANNL PP +VVNF+KTKT I+ VKIFDTN DIL AF+G 
Sbjct: 15  LTLILPFS-AAYQVGVNYGTVANNLPPPHQVVNFIKTKTIINHVKIFDTNHDILAAFSGI 74

Query: 73  SGILVTVTVGNGEIPALGDLAAARQWVAAHIAPFHPQTRINYIAVGNEIIHTNDKPLIAR 132
           +GI +TVTV N +I +L  L+ AR W++ ++ PF   T I YIAVGNE++ T+DK LI  
Sbjct: 75  TGISLTVTVPNSDIISLSKLSNARSWLSDNLLPFLLTTSIRYIAVGNEVVATSDKTLITH 134

Query: 133 LVPAMRSLHQALVLAGIKDIKVTSPHSLGILS-ISEPPSLGRFRRGYDRAIFGPMLQFLR 192
           L+PAM +L  AL LA +  I V++PHSLGILS  SEPPS G+FR+GYD+AIF P+L F  
Sbjct: 135 LLPAMETLTLALHLANVSRILVSTPHSLGILSGSSEPPSSGKFRKGYDKAIFSPILDFHN 194

Query: 193 ETKAPFMVNPYPYFGYSPKMANYALFQPNRGIY-DKYTKITYTNMFDGMMDALHSAMKKL 252
            TK+PFMVNPYPYFG+ P+  NYALF  N  +Y D  TK+ YTNMFD  +DA++SAMK+ 
Sbjct: 195 RTKSPFMVNPYPYFGFGPETLNYALFNTNDVVYVDPVTKLNYTNMFDAQLDAVYSAMKRF 254

Query: 253 GYADIDLVIGETGWPT--NCDYVACSLDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLF 312
           GY D+D+V+ ETGWP+    +     LD AA YN NLI HVNSGKGTPLMPNR FETY+F
Sbjct: 255 GYGDVDIVVAETGWPSAGEPNQTGVGLDYAAAYNGNLIKHVNSGKGTPLMPNRVFETYVF 314

Query: 313 GLFNENLKPGPTAERNWGLFQPDFTPVYDL 338
            LFNENLK    +E+N+GLF+PDFTPVYD+
Sbjct: 315 SLFNENLK-SSVSEQNFGLFKPDFTPVYDV 342

The following BLAST results are available for this feature:

BLAST of Lsi04G008770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52409	3.4e-125	51.49	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q9M069	1.4e-102	43.14	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q94G86	8.8e-97	42.86	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q8VYE5	3.8e-76	36.67	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q06915	8.6e-76	38.19	Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Lsi04G008770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS71	4.1e-238	90.13	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3...	[more]
A0A5D3BZM0	8.5e-236	87.86	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CF05	8.5e-236	87.86	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV...	[more]
A0A6J1GZM3	6.7e-233	85.90	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 P...	[more]
A0A6J1JYX6	3.7e-231	85.68	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=...	[more]

BLAST of Lsi04G008770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891662.1	7.8e-252	94.38	glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida]	[more]
XP_004147695.2	8.4e-238	89.93	glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN50054.2 hypothetical prot...	[more]
XP_008461659.1	1.8e-235	87.86	PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo] >KAA0050300.1 glucan ...	[more]
KAG7031616.1	4.8e-233	87.44	Glucan endo-1,3-beta-glucosidase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6601002.1	4.8e-233	86.12	Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of Lsi04G008770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55430.1	9.8e-144	56.69	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.1	1.3e-135	52.28	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.2	1.7e-135	52.30	O-Glycosyl hydrolases family 17 protein	[more]
AT2G39640.1	2.5e-123	50.35	glycosyl hydrolase family 17 protein	[more]
AT3G46570.1	3.3e-107	60.00	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 367..449 e-value: 1.6E-40 score: 150.6
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 367..437 e-value: 1.0E-19 score: 71.0
None	No IPR available	GENE3D	1.20.58.1040		coord: 365..455 e-value: 1.3E-10 score: 43.6
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 24..341 e-value: 1.3E-115 score: 387.8
None	No IPR available	PANTHER	PTHR32227:SF371	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE-LIKE	coord: 12..447
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 26..337 e-value: 1.2E-83 score: 281.3
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 12..447
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G008770.1	Lsi04G008770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds