Lsi04G008640 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G008640
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein of unknown function (DUF674)
Locationchr04: 8437258 .. 8439792 (-)
RNA-Seq ExpressionLsi04G008640
SyntenyLsi04G008640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGTAGCTATTTAACAGGGGAATTCTCTTCATTTTGGATGACTGTGTGATCGTTTTCTAATTATGTTTAGGTTCAAGTTACACATTAGTTTTTAACTTTCAATCTTGTATTTAAAATGTCACTATTCTTTGCATCCTTTCAATTCAATGAATTCATTCTTTCGGGAATGAAAATGTCTTTTTTCTTTTATTATCCATCTTAGGAAAGAGACGTAACTCTTTGAATGATCACGAATTAATTTATAAATATGTTATATGTCTCTCCCAAAATCTCAAAATTTCTATAAAAACTTTTCTCACATCTAAAAGTTTGTTGAAGACTGATTTAATTTACAAAGTAGAGTTCATTGGATCTTGTAGACGAGGTGTGTAATCTATTTTATCCTTGTAAATTTGTTGTACTCCGATTTTTTCATGCATACAGGTTTTGAAAAAAAAAATACAGGTTTTATATTTTCTGATGTAATGTGATGATATTCATATAACGTTTAACAAGTTATTGAACTTGTTTTAGGTTCTAAACTTTCAATTTAGTATCCAATAGGTCGGTAAATAAATAAGACAACTAAGACACGTTTGATACATAATTGAATGTTTAAAGATCTATTAAATACTTTGTAAAATTGAGAGACCATATAAATACAAATTTCAAAGTTTAGAGACTTAGTATACATTTTGTAAGGTTAACAAATCAAATAGACACAAATCCAATACTTCAAGACCTAAGCTTGTAATTTAACATTTTATCTTGCTTACGAATTCGAGCTAGTTAGAGATGTATTGCATTGCATGTTAGTATGTTTACTGCCATGGTTGATGTTCATTAAAGAAAATTAAAATTTGGGTTGCCTCATTTTGAAGGTTGACAACAATGGAACAAACCGATGTGAGATTGAAGCTTCTGATAGACACAAAAACAGAACGAGTTCTTTACGGTGAAGCAAACAAGAAGTTCATAGACTTTCTTTTCAATCTACTTTCCCTCCCAGTTGGGGCTGTAATTAGGCTGTTGAAAAAGGATGGCATGGTGGGGTGTTTGGGAAATCTGTACGAGAGTGTAGAAACCTTGAACGAGTCCTATTTGCAGCCAAATCAAAGCAAAGATATAGTCCTAAAACCCAAAGTCTTATTCAATTCTTTCACCAAACTTTTGGCTAATATTGTTGTCCCTGCTGCAGCTAAACCACCTGCTACATTTTATTGCCATAATGGATCATATAGCAGCTGCCGTGGTTACGTTTCTAATAGTTCTGATGCAATTTGTCCGAATTGTAACGGTCGCTTGAGTCAAAGTTGTAGATATGGACGCCTCCAAATGCAGGAATTCAACCATCTCCTGGTAGGGGATTTGTGAAGGATTTGGCCACTTACATAGTGATGGATGACCTTACTGTCAAGCACATTTCTGACTTCTCCATTACAACTCTTTTGAAAAAGTTCAATATCAAGGATGTGGATTCTTTGGAGGAGAAAGTCATCACTTTGGATGTCGATGAGGTAATTTATATATATCCACACGTTTTCATCTGTGTTCGAGGTGTAAATTATTTTAGTTTTAGTATGAAATAGTAAACACTATAACAAAAAAGGGAAAAGAGAGCATGAGTAGAAATAGTAAACACGATAACAAATAGGGATTTGAAATAGTCCCCTAGTTAATTAGGGGGCGTTTGGGGTGCTGAGTTGGTTATTATAGTGTGTTATAATAGTTAGTAGTGTTTTGAGGTGCAAACTACTTTAGTCTGGGTTATTAATAGTTTGTGTTTGGAGCGTAAATTATTTTTGTTCGGGTAGGAAATAGTAAACACCATAACAAAGAAGAAAAGAGAGGATAGGTGAAAAATAGTAAATACTATAGGTAAATGCGAGCAATGTTGTTTACTATAGTTAATTGTAGGTTATGAATAGTTATATAAACCACTATTATATTTGGTGCCCCAAACATGGAGTGAGTTATAAATTATAATAAACCCTAAACCCTTGTCCCAAAGAACCCCTTATAGGTTATAATAGTTGTAAGCACCAACTATTATAATTGTAACTCTAAATATGGAATGAGCTATATATAATAGCCCACTCCACCAACTTCAAGTTGCTGGCCCAAACATCTCCCTAGAGTTTAGAGTTAAGGGGAAAAAAATTAAATGAATTAGGTTACCCAATATCTAGGAATGATATAATCAAACCATCGAGACCAAATTAGAACTTTTAAACCCACGAGGACGAGAGACTACTAAAGTTGAAGTCCAAATCATGGTGACTAATTAAATTGAAAACTTTAAATCTAGAGATGAAATTGAAACTTTTTTCAAATGATGGACCAAATCTATAGTTCAACTATTATTTTATGTCTTTATGAACCCATGTTGGAAAATTTGATGAATAAGCTTGGTTTTTGTTGCAGGGTGTGGAATTACTAGAGGCTTCTTTGCAGTCAAAGACAGTTCTAACTAACGCGTTTCTGAAAAGACGAAGATCACACATTGACAGTAGTGATGTTAAGCTTTCTGAAGTTGTTAAGCCTTCTGTTTAA

mRNA sequence

ATGATGTTGACAACAATGGAACAAACCGATGTGAGATTGAAGCTTCTGATAGACACAAAAACAGAACGAGTTCTTTACGGTGAAGCAAACAAGAAGTTCATAGACTTTCTTTTCAATCTACTTTCCCTCCCAGTTGGGGCTGTAATTAGGCTGTTGAAAAAGGATGGCATGGTGGGGTGTTTGGGAAATCTGTACGAGAGTGTAGAAACCTTGAACGAGTCCTATTTGCAGCCAAATCAAAGCAAAGATATAGTCCTAAAACCCAAAGTCTTATTCAATTCTTTCACCAAACTTTTGGCTAATATTGTTGTCCCTGCTGCAGCTAAACCACCTGCTACATTTTATTGCCATAATGGATCATATAGCAGCTGCCGTGGTTACATATGGACGCCTCCAAATGCAGGAATTCAACCATCTCCTGGTAGGGGATTTGTGAAGGATTTGGCCACTTACATAGTGATGGATGACCTTACTGTCAAGCACATTTCTGACTTCTCCATTACAACTCTTTTGAAAAAGTTCAATATCAAGGATGTGGATTCTTTGGAGGAGAAAGGTGTGGAATTACTAGAGGCTTCTTTGCAGTCAAAGACAGTTCTAACTAACGCGTTTCTGAAAAGACGAAGATCACACATTGACAGTAGTGATGTTAAGCTTTCTGAAGTTGTTAAGCCTTCTGTTTAA

Coding sequence (CDS)

ATGATGTTGACAACAATGGAACAAACCGATGTGAGATTGAAGCTTCTGATAGACACAAAAACAGAACGAGTTCTTTACGGTGAAGCAAACAAGAAGTTCATAGACTTTCTTTTCAATCTACTTTCCCTCCCAGTTGGGGCTGTAATTAGGCTGTTGAAAAAGGATGGCATGGTGGGGTGTTTGGGAAATCTGTACGAGAGTGTAGAAACCTTGAACGAGTCCTATTTGCAGCCAAATCAAAGCAAAGATATAGTCCTAAAACCCAAAGTCTTATTCAATTCTTTCACCAAACTTTTGGCTAATATTGTTGTCCCTGCTGCAGCTAAACCACCTGCTACATTTTATTGCCATAATGGATCATATAGCAGCTGCCGTGGTTACATATGGACGCCTCCAAATGCAGGAATTCAACCATCTCCTGGTAGGGGATTTGTGAAGGATTTGGCCACTTACATAGTGATGGATGACCTTACTGTCAAGCACATTTCTGACTTCTCCATTACAACTCTTTTGAAAAAGTTCAATATCAAGGATGTGGATTCTTTGGAGGAGAAAGGTGTGGAATTACTAGAGGCTTCTTTGCAGTCAAAGACAGTTCTAACTAACGCGTTTCTGAAAAGACGAAGATCACACATTGACAGTAGTGATGTTAAGCTTTCTGAAGTTGTTAAGCCTTCTGTTTAA

Protein sequence

MMLTTMEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLYESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAAKPPATFYCHNGSYSSCRGYIWTPPNAGIQPSPGRGFVKDLATYIVMDDLTVKHISDFSITTLLKKFNIKDVDSLEEKGVELLEASLQSKTVLTNAFLKRRRSHIDSSDVKLSEVVKPSV
Homology
BLAST of Lsi04G008640 vs. ExPASy TrEMBL
Match: A0A5A7V731 (Putative DNA polymerase zeta catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00410 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 6.0e-89
Identity = 185/251 (73.71%), Postives = 198/251 (78.88%), Query Frame = 0

Query: 6   MEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLY 65
           MEQTDV LKLLID KTERVLYGEA+KKFIDFL N+LSLP+G VI LLKK+GMVGCLGNLY
Sbjct: 1   MEQTDVTLKLLIDKKTERVLYGEADKKFIDFLVNVLSLPLGGVIMLLKKNGMVGCLGNLY 60

Query: 66  ESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAAKPPATFYCHNGSYSSCR 125
           ESVETLN+SYLQPNQS+D VLKPK+LFNSFTKLL N+ VPAAA PPA FYC+  +YSSCR
Sbjct: 61  ESVETLNKSYLQPNQSRDTVLKPKILFNSFTKLLPNVHVPAAATPPAIFYCNGSTYSSCR 120

Query: 126 GYI---------------------WTPPNAGIQPSPGRGFVKDLATYIVMDDLTVKHISD 185
            Y+                      TPP A  QP+ G G VKDLATYIVMDDLTVKHISD
Sbjct: 121 AYVSYSSSAVCPKCDKKLSQSCTYVTPPKAENQPASGGGSVKDLATYIVMDDLTVKHISD 180

Query: 186 FSITTLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRRRSHIDSSD 228
           FSITTLLKKFNIKDVDSLEEK        GVELLEASLQSKTVLTNAFLKRRR HID SD
Sbjct: 181 FSITTLLKKFNIKDVDSLEEKVITLDVDEGVELLEASLQSKTVLTNAFLKRRRPHID-SD 240

BLAST of Lsi04G008640 vs. ExPASy TrEMBL
Match: A0A1S3CPD6 (uncharacterized protein LOC103503094 OS=Cucumis melo OX=3656 GN=LOC103503094 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 6.0e-89
Identity = 185/251 (73.71%), Postives = 198/251 (78.88%), Query Frame = 0

Query: 6   MEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLY 65
           MEQTDV LKLLID KTERVLYGEA+KKFIDFL N+LSLP+G VI LLKK+GMVGCLGNLY
Sbjct: 1   MEQTDVTLKLLIDKKTERVLYGEADKKFIDFLVNVLSLPLGGVIMLLKKNGMVGCLGNLY 60

Query: 66  ESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAAKPPATFYCHNGSYSSCR 125
           ESVETLN+SYLQPNQS+D VLKPK+LFNSFTKLL N+ VPAAA PPA FYC+  +YSSCR
Sbjct: 61  ESVETLNKSYLQPNQSRDTVLKPKILFNSFTKLLPNVHVPAAATPPAIFYCNGSTYSSCR 120

Query: 126 GYI---------------------WTPPNAGIQPSPGRGFVKDLATYIVMDDLTVKHISD 185
            Y+                      TPP A  QP+ G G VKDLATYIVMDDLTVKHISD
Sbjct: 121 AYVSYSSSAVCPKCDKKLSQSCTYVTPPKAENQPASGGGSVKDLATYIVMDDLTVKHISD 180

Query: 186 FSITTLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRRRSHIDSSD 228
           FSITTLLKKFNIKDVDSLEEK        GVELLEASLQSKTVLTNAFLKRRR HID SD
Sbjct: 181 FSITTLLKKFNIKDVDSLEEKVITLDVDEGVELLEASLQSKTVLTNAFLKRRRPHID-SD 240

BLAST of Lsi04G008640 vs. ExPASy TrEMBL
Match: A0A6J1CBJ8 (uncharacterized protein LOC111010013 OS=Momordica charantia OX=3673 GN=LOC111010013 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 5.8e-52
Identity = 129/240 (53.75%), Postives = 162/240 (67.50%), Query Frame = 0

Query: 6   MEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLY 65
           M   +VRLKLLID+K +RVL+GEA+K  IDFLFNLLSLP+G VIRLLKK GMVGCLGNLY
Sbjct: 1   MASANVRLKLLIDSKEQRVLFGEADKNVIDFLFNLLSLPLGTVIRLLKKQGMVGCLGNLY 60

Query: 66  ESVETLNESYLQPNQSKDIVLKPKVLF--NSFTKLLANIVVPAAAKPPATFY-CHNGSYS 125
           ESVETLN++YLQPNQSKDI+LKPKV F  +S T LL NI + AAA    TFY C++ +++
Sbjct: 61  ESVETLNDTYLQPNQSKDILLKPKVSFCGSSSTMLLPNIDLSAAA---TTFYLCNSTAHA 120

Query: 126 SCRGYIWTPPNA--------------GIQPSPGR------------GFVKDLATYIVMDD 185
           +CR  +   PNA               ++P                GFVK + TY+VMDD
Sbjct: 121 NCRRSVSDGPNAICPKCNVPMTQVGTFVKPPSASATTVAAAKEDEGGFVKGVVTYMVMDD 180

Query: 186 LTVKHISDFSITTLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRR 209
           L+VK +S  S   LL KFN+K+V +LEEK        GV+LL+ASL SKTVLT+ F++R+
Sbjct: 181 LSVKPMSTISSIALLNKFNVKEVGALEEKVVTLDVNEGVKLLKASLHSKTVLTDVFIRRK 237

BLAST of Lsi04G008640 vs. ExPASy TrEMBL
Match: A0A5A7U8V2 (DUF674 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3341G00030 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 1.3e-48
Identity = 125/228 (54.82%), Postives = 149/228 (65.35%), Query Frame = 0

Query: 10  DVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLYESVE 69
           +VRLKLLID++ +RVL+GEA+K  IDFLFNLLSLP+G VIRLLKK GMVG L NLYESVE
Sbjct: 7   EVRLKLLIDSEQKRVLFGEADKNLIDFLFNLLSLPLGTVIRLLKKQGMVGSLANLYESVE 66

Query: 70  TLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPA----------------AAKPPAT 129
            LN++YLQPNQSKD +LKPKV F++ T LL NI   A                A+ P A 
Sbjct: 67  ALNDTYLQPNQSKDNLLKPKVSFSASTLLLPNIESYADKKKFYLCGNRCGYNVASNPTAV 126

Query: 130 F-YCHNGSYSSCRGYIWTPPNAGIQPSPG----RGFVKDLATYIVMDDLTVKHISDFSIT 189
              C N     C   +  PPNA  Q +       GFVK + TY+VMDDL+VK +S  S  
Sbjct: 127 CPSCRNAMSRECA--LVNPPNANTQATQDVGEFGGFVKGVVTYMVMDDLSVKPMSTISSI 186

Query: 190 TLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRR 209
           TLL KFNIK+V +LEEK        GV+LL ASLQSKTVLT+ FL R+
Sbjct: 187 TLLNKFNIKEVGALEEKVITLDVNQGVKLLRASLQSKTVLTDVFLGRK 232

BLAST of Lsi04G008640 vs. ExPASy TrEMBL
Match: A0A1S3CGQ2 (uncharacterized protein LOC103500268 OS=Cucumis melo OX=3656 GN=LOC103500268 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 1.3e-48
Identity = 125/228 (54.82%), Postives = 149/228 (65.35%), Query Frame = 0

Query: 10  DVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLYESVE 69
           +VRLKLLID++ +RVL+GEA+K  IDFLFNLLSLP+G VIRLLKK GMVG L NLYESVE
Sbjct: 7   EVRLKLLIDSEQKRVLFGEADKNLIDFLFNLLSLPLGTVIRLLKKQGMVGSLANLYESVE 66

Query: 70  TLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPA----------------AAKPPAT 129
            LN++YLQPNQSKD +LKPKV F++ T LL NI   A                A+ P A 
Sbjct: 67  ALNDTYLQPNQSKDNLLKPKVSFSASTLLLPNIESYADKKKFYLCGNRCGYNVASNPTAV 126

Query: 130 F-YCHNGSYSSCRGYIWTPPNAGIQPSPG----RGFVKDLATYIVMDDLTVKHISDFSIT 189
              C N     C   +  PPNA  Q +       GFVK + TY+VMDDL+VK +S  S  
Sbjct: 127 CPSCRNAMSRECA--LVNPPNANTQATQDVGEFGGFVKGVVTYMVMDDLSVKPMSTISSI 186

Query: 190 TLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRR 209
           TLL KFNIK+V +LEEK        GV+LL ASLQSKTVLT+ FL R+
Sbjct: 187 TLLNKFNIKEVGALEEKVITLDVNQGVKLLRASLQSKTVLTDVFLGRK 232

BLAST of Lsi04G008640 vs. NCBI nr
Match: XP_031741245.1 (uncharacterized protein LOC105435653 [Cucumis sativus] >KAE8647978.1 hypothetical protein Csa_000490 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-89
Identity = 189/252 (75.00%), Postives = 201/252 (79.76%), Query Frame = 0

Query: 6   MEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLY 65
           MEQTDV LKLLID KTERVLYGEA+KKFIDFL N+LSLP+G VIRLLKKDGMVGCLGNLY
Sbjct: 1   MEQTDVTLKLLIDKKTERVLYGEADKKFIDFLVNVLSLPLGGVIRLLKKDGMVGCLGNLY 60

Query: 66  ESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANI-VVPAAAKPPATFYCHNGSYSSC 125
           ESVETLN SYLQPNQS+DIVLKPK++FNSFTKL+ N+ VVPAAA PPA FYC  GSY  C
Sbjct: 61  ESVETLNNSYLQPNQSRDIVLKPKIVFNSFTKLMPNVDVVPAAATPPAIFYCVTGSY--C 120

Query: 126 RGYI---------------------WTPPNAGIQPSPGRGFVKDLATYIVMDDLTVKHIS 185
           RGY+                      TPP AG QP+P RGFVKDLATYIV DDLTVKHIS
Sbjct: 121 RGYVSYSCSAFCPSCSKNLSQACRYVTPPKAGNQPAPDRGFVKDLATYIVTDDLTVKHIS 180

Query: 186 DFSITTLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRRRSHIDSS 228
           DFSITTLLKKFNIKDVDSLEEK        GVELLEASLQSKTVLTNAFLKRRRSHID +
Sbjct: 181 DFSITTLLKKFNIKDVDSLEEKVITLDVNEGVELLEASLQSKTVLTNAFLKRRRSHID-N 240

BLAST of Lsi04G008640 vs. NCBI nr
Match: XP_008465479.1 (PREDICTED: uncharacterized protein LOC103503094 [Cucumis melo] >KAA0062236.1 putative DNA polymerase zeta catalytic subunit [Cucumis melo var. makuwa])

HSP 1 Score: 337.0 bits (863), Expect = 1.2e-88
Identity = 185/251 (73.71%), Postives = 198/251 (78.88%), Query Frame = 0

Query: 6   MEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLY 65
           MEQTDV LKLLID KTERVLYGEA+KKFIDFL N+LSLP+G VI LLKK+GMVGCLGNLY
Sbjct: 1   MEQTDVTLKLLIDKKTERVLYGEADKKFIDFLVNVLSLPLGGVIMLLKKNGMVGCLGNLY 60

Query: 66  ESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAAKPPATFYCHNGSYSSCR 125
           ESVETLN+SYLQPNQS+D VLKPK+LFNSFTKLL N+ VPAAA PPA FYC+  +YSSCR
Sbjct: 61  ESVETLNKSYLQPNQSRDTVLKPKILFNSFTKLLPNVHVPAAATPPAIFYCNGSTYSSCR 120

Query: 126 GYI---------------------WTPPNAGIQPSPGRGFVKDLATYIVMDDLTVKHISD 185
            Y+                      TPP A  QP+ G G VKDLATYIVMDDLTVKHISD
Sbjct: 121 AYVSYSSSAVCPKCDKKLSQSCTYVTPPKAENQPASGGGSVKDLATYIVMDDLTVKHISD 180

Query: 186 FSITTLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRRRSHIDSSD 228
           FSITTLLKKFNIKDVDSLEEK        GVELLEASLQSKTVLTNAFLKRRR HID SD
Sbjct: 181 FSITTLLKKFNIKDVDSLEEKVITLDVDEGVELLEASLQSKTVLTNAFLKRRRPHID-SD 240

BLAST of Lsi04G008640 vs. NCBI nr
Match: XP_022138964.1 (uncharacterized protein LOC111010013 [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-51
Identity = 129/240 (53.75%), Postives = 162/240 (67.50%), Query Frame = 0

Query: 6   MEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLY 65
           M   +VRLKLLID+K +RVL+GEA+K  IDFLFNLLSLP+G VIRLLKK GMVGCLGNLY
Sbjct: 1   MASANVRLKLLIDSKEQRVLFGEADKNVIDFLFNLLSLPLGTVIRLLKKQGMVGCLGNLY 60

Query: 66  ESVETLNESYLQPNQSKDIVLKPKVLF--NSFTKLLANIVVPAAAKPPATFY-CHNGSYS 125
           ESVETLN++YLQPNQSKDI+LKPKV F  +S T LL NI + AAA    TFY C++ +++
Sbjct: 61  ESVETLNDTYLQPNQSKDILLKPKVSFCGSSSTMLLPNIDLSAAA---TTFYLCNSTAHA 120

Query: 126 SCRGYIWTPPNA--------------GIQPSPGR------------GFVKDLATYIVMDD 185
           +CR  +   PNA               ++P                GFVK + TY+VMDD
Sbjct: 121 NCRRSVSDGPNAICPKCNVPMTQVGTFVKPPSASATTVAAAKEDEGGFVKGVVTYMVMDD 180

Query: 186 LTVKHISDFSITTLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRR 209
           L+VK +S  S   LL KFN+K+V +LEEK        GV+LL+ASL SKTVLT+ F++R+
Sbjct: 181 LSVKPMSTISSIALLNKFNVKEVGALEEKVVTLDVNEGVKLLKASLHSKTVLTDVFIRRK 237

BLAST of Lsi04G008640 vs. NCBI nr
Match: XP_008461735.1 (PREDICTED: uncharacterized protein LOC103500268 [Cucumis melo] >KAA0052182.1 DUF674 domain-containing protein [Cucumis melo var. makuwa] >TYJ97778.1 DUF674 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 203.0 bits (515), Expect = 2.8e-48
Identity = 125/228 (54.82%), Postives = 149/228 (65.35%), Query Frame = 0

Query: 10  DVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLYESVE 69
           +VRLKLLID++ +RVL+GEA+K  IDFLFNLLSLP+G VIRLLKK GMVG L NLYESVE
Sbjct: 7   EVRLKLLIDSEQKRVLFGEADKNLIDFLFNLLSLPLGTVIRLLKKQGMVGSLANLYESVE 66

Query: 70  TLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPA----------------AAKPPAT 129
            LN++YLQPNQSKD +LKPKV F++ T LL NI   A                A+ P A 
Sbjct: 67  ALNDTYLQPNQSKDNLLKPKVSFSASTLLLPNIESYADKKKFYLCGNRCGYNVASNPTAV 126

Query: 130 F-YCHNGSYSSCRGYIWTPPNAGIQPSPG----RGFVKDLATYIVMDDLTVKHISDFSIT 189
              C N     C   +  PPNA  Q +       GFVK + TY+VMDDL+VK +S  S  
Sbjct: 127 CPSCRNAMSRECA--LVNPPNANTQATQDVGEFGGFVKGVVTYMVMDDLSVKPMSTISSI 186

Query: 190 TLLKKFNIKDVDSLEEK--------GVELLEASLQSKTVLTNAFLKRR 209
           TLL KFNIK+V +LEEK        GV+LL ASLQSKTVLT+ FL R+
Sbjct: 187 TLLNKFNIKEVGALEEKVITLDVNQGVKLLRASLQSKTVLTDVFLGRK 232

BLAST of Lsi04G008640 vs. NCBI nr
Match: XP_038891339.1 (uncharacterized protein LOC120080784 [Benincasa hispida] >XP_038891341.1 uncharacterized protein LOC120080784 [Benincasa hispida])

HSP 1 Score: 199.9 bits (507), Expect = 2.3e-47
Identity = 129/275 (46.91%), Postives = 163/275 (59.27%), Query Frame = 0

Query: 7   EQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDGMVGCLGNLYE 66
           + T+VRLKLLIDT+   VLYGEA+K FIDFLFNLLSLP+GAVIRLL K  M+GCLGNLYE
Sbjct: 4   QTTNVRLKLLIDTEKRSVLYGEADKDFIDFLFNLLSLPLGAVIRLLTKQHMIGCLGNLYE 63

Query: 67  SVETLNES-YLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAAK------PPATFYCHN- 126
           S+ETLNE+ +++P QSK+ +L+PKV  N  TKLL  I+   ++       P ++ + H  
Sbjct: 64  SIETLNETCFIKPRQSKETLLRPKVSLNCSTKLLPYIIDQTSSSSIGDNAPNSSTWSHGC 123

Query: 127 -----------------GSYSSCRGYIWT--PPNA-------------GIQPSPGR---- 186
                            G+ SS   + ++  PP++             G   +P      
Sbjct: 124 SSFGAHSTTLSDSCSLFGAASSTSSFCFSTIPPSSFSLGSLSSGFSSIGCSSTPIESGST 183

Query: 187 ---------------GFVKDLATYIVMDDLTVKHISDFSITTLLKKFNIKDVDSLEEK-- 215
                          GFVK LATYIVMDDLTVKHISDFSI +L +KFNIKD  +LEEK  
Sbjct: 184 RCEASSSSGGGGLKVGFVKGLATYIVMDDLTVKHISDFSIVSLFRKFNIKDAYTLEEKVI 243

BLAST of Lsi04G008640 vs. TAIR 10
Match: AT5G01130.1 (Protein of unknown function (DUF674) )

HSP 1 Score: 79.7 bits (195), Expect = 3.3e-15
Identity = 69/230 (30.00%), Postives = 105/230 (45.65%), Query Frame = 0

Query: 2   MLTTMEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKKDG----- 61
           M  + E+  V L+L ID +  +V+  EA+K F+D LF+LL+LP+G +IRLL++       
Sbjct: 1   MAESSEEPKVSLRLFIDEEKNKVVLAEASKAFVDVLFSLLTLPMGTIIRLLEEHRESQPI 60

Query: 62  MVGCLGNLYESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAAK-----PP 121
            VGC  NLY SV  +     + +  K I+L P+ + +   K L   + P   K       
Sbjct: 61  TVGCFSNLYRSVAEMGIDNFKTDVCKHILLHPRSVRDLQYKRLMLNINPTEVKLFKCPIS 120

Query: 122 ATFYCHNGSYSSCRGYIWTPPNAGIQPS---PGRGFVKD--LATYIVMDDLTVKHISDFS 181
             FY H   +S+ R    +  N   Q     P    +++     +I+ DDL V   S   
Sbjct: 121 CNFYSH---FSTSRCQCGSSMNKEFQEPRVVPVASSIQNDVNGVFIITDDLKVAVRSTDV 180

Query: 182 ITTLLKKFNIKDVDSLE--------EKGVELLEASLQSKTVLTNAFLKRR 209
           +   LK     D+  L         E+ + LLE    SK  LTN FL ++
Sbjct: 181 VLNELKSLGSGDISKLREMLVYIGFEEVLTLLECMFSSKAPLTNTFLNKQ 227

BLAST of Lsi04G008640 vs. TAIR 10
Match: AT5G01120.1 (Protein of unknown function (DUF674) )

HSP 1 Score: 73.2 bits (178), Expect = 3.1e-13
Identity = 68/244 (27.87%), Postives = 106/244 (43.44%), Query Frame = 0

Query: 2   MLTTMEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLK-----KDG 61
           M    E + + LKLLID +  +V++ EA   F+D LF+  +LP+G ++RLL+     +  
Sbjct: 1   MSDDFENSVINLKLLIDEEKNKVVFAEAGTDFVDILFSFSTLPMGTIVRLLEMHHKSRSI 60

Query: 62  MVGCLGNLYESVETLNESYLQPNQSKDIVLKP-------------KVLFNSFTKLLANIV 121
            +GC  N+Y SV ++   +      K ++L P             K+  +  TK     +
Sbjct: 61  AIGCFNNIYASVVSMGMKHFSTQACKQMLLFPGSLNQDKCQNMKLKIDDSEATKCFMCPM 120

Query: 122 VPAAAKPPATFYCHNGSYSSCRGYIWTPPNAGIQPSPGRG-------FVKDLAT-YIVMD 181
              + +    +     S  SC  ++        Q   GRG       FV+   T +I+ D
Sbjct: 121 FVRSGQCSKGYSNFKTSRCSCGNFM--DEVIQFQGEGGRGNGSQAEVFVRGAHTSFIITD 180

Query: 182 DLTVKHISDFSITTLLKKFNIKDVDSLEEKGVE--------LLEASLQSKTVLTNAFLKR 212
           DL V+  S  S   +LK     D D L E  +E        LL     S T LT+ FLK+
Sbjct: 181 DLKVQVNSVGSTLNVLKDLAYADCDKLVEMILEVNLQEVATLLVCLFTSDTPLTDTFLKK 240

BLAST of Lsi04G008640 vs. TAIR 10
Match: AT3G09110.1 (Protein of unknown function (DUF674) )

HSP 1 Score: 72.8 bits (177), Expect = 4.0e-13
Identity = 67/234 (28.63%), Postives = 102/234 (43.59%), Query Frame = 0

Query: 2   MLTTMEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLKK-----DG 61
           M  + E+    L+LLID +  RV+  EA K F+D L +LL+LP+G ++RLL+K       
Sbjct: 1   MAKSFEKPKFSLRLLIDEEKNRVILAEAGKDFVDVLCSLLTLPMGTIVRLLEKHQNPQSS 60

Query: 62  MVGCLGNLYESVETLNESYLQPNQSKDIVLKPKVLFNSFTK-LLANIVVPAAAKPPATFY 121
           +VGCL NLY+SV  ++    +    K  +L P+    S  + L  NI    A K    F 
Sbjct: 61  VVGCLHNLYKSVADMDVDNFESQACKHFLLHPRSAKGSHGRDLKLNIDDTEATK---FFV 120

Query: 122 C-------------HNGSYSSCRGYIWTPPNAGIQPSPGRG-FVKDLATYIVMDDLTVKH 181
           C              N S   CR          ++     G F     ++++ DDL V  
Sbjct: 121 CPNFVSTEACRKLFSNVSTMKCRCGSSMHREIPVEEQQADGVFPSCRTSFVITDDLKVAL 180

Query: 182 ISDFSITTLLKKFNIKDVDSLEEKGVE--------LLEASLQSKTVLTNAFLKR 208
            S   +  +L  F     D L+E  ++        LL     S+  LT+ FL++
Sbjct: 181 NSMGLVLNVLNDFGYSGFDKLQEMLIDVGFEEILTLLGCLFTSEAPLTDTFLRK 231

BLAST of Lsi04G008640 vs. TAIR 10
Match: AT5G43240.1 (Protein of unknown function (DUF674) )

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-12
Identity = 68/245 (27.76%), Postives = 112/245 (45.71%), Query Frame = 0

Query: 2   MLTTMEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLK-----KDG 61
           M  T++   ++LKLLID +  +V++ EA K F+D LF+  +LP+G ++RLL+     +  
Sbjct: 1   MSATLKNPVIKLKLLIDEEKNKVVFVEAGKDFVDLLFSFFTLPMGTIVRLLEMYKKSQKI 60

Query: 62  MVGCLGNLYESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAA-------- 121
            +GC  N+Y SV ++   +      K ++L P  L +   + L   V  + A        
Sbjct: 61  AIGCFSNIYASVVSMGIEHFLTEACKQMLLYPGSLNHEKCRNLKLRVDDSEATKYFVCPK 120

Query: 122 -----KPPATFYCHNGSYSSC---RGYIWTPPNAGIQPSPGRG-----FVK-DLATYIVM 181
                +   ++   N S  SC      +      G   S G G     FV+ D  ++++ 
Sbjct: 121 FVEREQCTESYSNFNTSRCSCGVLMNEVTQLDGRGGLASAGNGVEGGVFVRSDHTSFMIT 180

Query: 182 DDLTVKHISDFSITTLLKKFNIKDVDSLEEK----GVE----LLEASLQSKTVLTNAFLK 212
           DDL V+  S      +LK     D + L+EK     +E    LLE    S   LT+ FLK
Sbjct: 181 DDLKVEITSVRLTLNVLKDLGYVDCEKLDEKIHDVNLEEVPTLLECLSTSDNPLTDTFLK 240

BLAST of Lsi04G008640 vs. TAIR 10
Match: AT5G43240.3 (Protein of unknown function (DUF674) )

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-12
Identity = 68/245 (27.76%), Postives = 112/245 (45.71%), Query Frame = 0

Query: 2   MLTTMEQTDVRLKLLIDTKTERVLYGEANKKFIDFLFNLLSLPVGAVIRLLK-----KDG 61
           M  T++   ++LKLLID +  +V++ EA K F+D LF+  +LP+G ++RLL+     +  
Sbjct: 1   MSATLKNPVIKLKLLIDEEKNKVVFVEAGKDFVDLLFSFFTLPMGTIVRLLEMYKKSQKI 60

Query: 62  MVGCLGNLYESVETLNESYLQPNQSKDIVLKPKVLFNSFTKLLANIVVPAAA-------- 121
            +GC  N+Y SV ++   +      K ++L P  L +   + L   V  + A        
Sbjct: 61  AIGCFSNIYASVVSMGIEHFLTEACKQMLLYPGSLNHEKCRNLKLRVDDSEATKYFVCPK 120

Query: 122 -----KPPATFYCHNGSYSSC---RGYIWTPPNAGIQPSPGRG-----FVK-DLATYIVM 181
                +   ++   N S  SC      +      G   S G G     FV+ D  ++++ 
Sbjct: 121 FVEREQCTESYSNFNTSRCSCGVLMNEVTQLDGRGGLASAGNGVEGGVFVRSDHTSFMIT 180

Query: 182 DDLTVKHISDFSITTLLKKFNIKDVDSLEEK----GVE----LLEASLQSKTVLTNAFLK 212
           DDL V+  S      +LK     D + L+EK     +E    LLE    S   LT+ FLK
Sbjct: 181 DDLKVEITSVRLTLNVLKDLGYVDCEKLDEKIHDVNLEEVPTLLECLSTSDNPLTDTFLK 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V7316.0e-8973.71Putative DNA polymerase zeta catalytic subunit OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CPD66.0e-8973.71uncharacterized protein LOC103503094 OS=Cucumis melo OX=3656 GN=LOC103503094 PE=... [more]
A0A6J1CBJ85.8e-5253.75uncharacterized protein LOC111010013 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A5A7U8V21.3e-4854.82DUF674 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CGQ21.3e-4854.82uncharacterized protein LOC103500268 OS=Cucumis melo OX=3656 GN=LOC103500268 PE=... [more]
Match NameE-valueIdentityDescription
XP_031741245.11.5e-8975.00uncharacterized protein LOC105435653 [Cucumis sativus] >KAE8647978.1 hypothetica... [more]
XP_008465479.11.2e-8873.71PREDICTED: uncharacterized protein LOC103503094 [Cucumis melo] >KAA0062236.1 put... [more]
XP_022138964.11.2e-5153.75uncharacterized protein LOC111010013 [Momordica charantia][more]
XP_008461735.12.8e-4854.82PREDICTED: uncharacterized protein LOC103500268 [Cucumis melo] >KAA0052182.1 DUF... [more]
XP_038891339.12.3e-4746.91uncharacterized protein LOC120080784 [Benincasa hispida] >XP_038891341.1 unchara... [more]
Match NameE-valueIdentityDescription
AT5G01130.13.3e-1530.00Protein of unknown function (DUF674) [more]
AT5G01120.13.1e-1327.87Protein of unknown function (DUF674) [more]
AT3G09110.14.0e-1328.63Protein of unknown function (DUF674) [more]
AT5G43240.11.2e-1227.76Protein of unknown function (DUF674) [more]
AT5G43240.31.2e-1227.76Protein of unknown function (DUF674) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007750Protein of unknown function DUF674PFAMPF05056DUF674coord: 8..125
e-value: 3.6E-24
score: 85.5
coord: 143..214
e-value: 1.7E-9
score: 37.1
IPR007750Protein of unknown function DUF674PANTHERPTHR33103OS01G0153900 PROTEINcoord: 8..208
NoneNo IPR availablePANTHERPTHR33103:SF50OS01G0154200 PROTEINcoord: 8..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G008640.1Lsi04G008640.1mRNA