Lsi04G008540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G008540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	glutathione S-transferase DHAR2-like
Location	chr04: 8304460 .. 8307933 (+)
RNA-Seq Expression	Lsi04G008540
Synteny	Lsi04G008540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGGAATGGACACTTGAGAAGAGATATGCTCTGCGGGCAAGGAGAATCTGACACTTAAACTTTAAACGTGGTTGTATATGGTTCATATTCAATGGTCATTTGGTCCATGCCCAATTTCTTTTCAAAAGAATAAAATAATGAGAGAAACTTTCGTTTATTCTTCATAAATCATTGGGATATCAAATGGGTAATTGCTAGAAATAGTTCTCCACATGTGACAAAGGCCACAGAGCTTCAATATATACCCACCTTCCCTTCAATCGCCATTCACATTTAAGTTTCTTCCGTATTCCCAGATTCAAACATTTCCCCACTACCCATTTCATACGAAAAATGGTCCTCGAAATTGCGGTCAAGGCTGCCACTGGTTCACCTGATGTGGTTGGGGACTGTATGTTCTGCTTTCTCTCCCTCTCACTTTACAAATATGTGTCAGTTTTTGTCAATTTTGCGATTTGGGGTTTTGTTTTTCTTTCTCTTGCAGGCCCTTTCTGCCATAGGGTTGTCTTAACTCTGGAGGAGAAGAAAGTTCCTTACAAATTGCTTCTCATCAATCTATCCGACAAACCCAGTTGGTAATATCTCGAAATTTGGCTTGTATTTGTTATTTGGGTTGCAGCGAGAGAAAGAGGGTGTTAATCAAATGGGGTTGTGTGTGGTGGATTGTTGATGATTTTGGCAGGTTATTGGAAGTGAACCCAGAAGGGAAGGTGCCGGTGGCGAAGTTTGATGGCAAATGGGTTCCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTTGTTACTCCTCCTCAATTTTCTTCTGTGTAAGCCTTCTTTTTTTCTTTGACCCTTTTTTCTGATTTGATCATTTGTTCCATATCTGAGGATATTTTAATGTATGAGGTTGCCTTTGTGTCTTGTGTTTGCTGTTTCCTGTGCTTTTATGTTAATTACTGTTTTCAACAGTTCATATTTGAAGTTTTTATTTGTTGAGTTGTGATAATTCCTGTTGGGACAGGGATGATTGTTACCTCGTGTTTGTGTTTGTTTGATGTGTCCATTTTTCTCATGTCTCACTGCCTTCTAGTTTGACCCTTATTTTAACAGGATGTTTGGTCCCGTCTTGTTGGAGTGGACCTAAATTTGTCTTATGATTTGTGGAAACCATGTTTGATGTTTATTTTCTTCTCGTATGGAGTCAGACTGTCAGGTAGGTGTTTTTTTAAACTAGAATTTCATTGATAAAAACAATATGTACAGAGGATTCCAAGGATTAAAAAAGAATATGTAAAAAAATCAAGTGGGAAACCATCAAGGAAACGTGTGCTGAAACACCAAACTCCAAAGTGTTTCCTTATCTCCACCTGCTCTCTTTTGATCCAATTGCTTCTTTCATCGAATTGTTCAAAACAATAGTCTTCTTTTCAGTGGCAGCTGAATGTTTTTATATTGGAGGGGCATGATTGTATTACATTAAAACATTAGGTAGGTTCAGGATCTTGATGTCCATTATCTAGATGAGGAAATCTTGAAGGACCGAAAACACTTCCCACATGAAATCACCATGTTTCGGAGTAAAGTTTATGAACTTTAAAACGCTTTCTGAAAAAGTACTTGTAAAGTGTTTTTTGGCCGAGGTAGAGGTACCTGTGGCCCAAATTCGTGAGTTTGAATAGGCCATAGGGTCGGGTAGACATTCCATTCCCCTAAGTTGTAGGTGCTCATTTGATTTTGTAAGTGATTGACTGGGAAGAACTAAATAGTAAAGAGTACTTTTATTGAAAAATAGAACCAAACATTCAAGTAATTTGTTATTAAACACTTCTTATTAAAGTGATTACAATTAAAATACTATGCTAAGAGCACTTATTTTGAAATCACTCCAAAAACACCTCTAACTTCAAACAGTTTTGGATGAATAGTTAACATCATGATTCATTATTTGATTTGCCTGAATTTCTTATTTCTGTACCTTTTAACCAACAAAGAAAGAGTGATTCATTCTACAGTCGACATGTCATGTCATGGAACAAGAATTGACTGCACGATTAGGACATGATCCCACATTGAGATTGAACTTATAACGATGGTTGAAATAAACTCATGATCTATGTATGTGTTTTGCTTGAATTTGTACTCTTCTTTATATTATTTGTCTCAGTAGTATTGCCTAATTCAGTACTCCGAATGTGGCATTTGCAAGATTGTAATTGTGAAGGCTTTCCTAAACTAATCACAAACTATTATTTTGTTCTGAAATAATTTGTCTTCTCTCTCCTTTGGCTAATAGAGGATCAAAGATGTTTGGTGCTTTTGTAAAATTCTTGTTAAGCAAGGACCCGAATGATGGTACAGAGCAGGCTTTCCTTGAAGAGTTGAAGGCGCTCAATGAGCATCTTAAGGCTCATGTATGTATGTGTAATATGTAGAACAACTATGTTAAAGTGTTTATCATTTTATCTTCTCTTTTAAAGCAATTTTACATGGTGCCAGGGTCCTTATATTGCTGGAGAGAAGGTAACTGCAGTTGATTTGAGTCTGGCACCAAAGTTGTTCCATGTTGATGTTGCTCTTGGCCATTTTAAGAAGTGGTCTATCCCCGAAGACTTGGCCAGTTTAATCGCCTACAAAGAGGTACGATGTTGTTGCACAAATGCTACGTAACTCGTGACTTTTAATTCAATTAAATCCTTTTAAGCCAGTATCAGATTTATATTGGTTATTAGTTTTGTTTGTTCACTCTCTGTTTAGACTTTGCTAGCTTCTTAGATTGCCTACAGTTTGAAACCTATTAGGCTGATTGGTTTCTGCTCAACTAAACTCAAACCTGCAGCAATGTGGCGTGTGTCTAATTGATGGAGGATCATAGAACATACTTTGTTCTTTAATTTAGGTTGTTATGCACAAATCAGATATAATCCTAATGAGTGGGATGTGAGTTCATCTAGTTACTTAGAGTTCGATGTTCGAGATTATCAGGGTCGATATTTGTTCAATTCGGGGGGGGGGGGGGGGGGNGAAAAAGAATGCAATAGATGAGTTGTCTGATGGTCTGATGAACTTTTGGTGGTGGGATTGTGGATTTTGCAGTTGCTTTTTGCTCGAGAATCTTTCGTGAAGACGAAAACTGCCCCGGAATATGTAATTGCTGGATGGGGGCCGAAGGTGAATCCTGCATGAATTGGGAGATGTTTTCATGTCAAGTTATGAAATAATTGGGGGATTTTAATCCTCTTAAGAATGTGGCTGTGGTTGCTTGCTATCTTCTTATGTGTCAATAAATAGTGTATTTTTCATATGTGTGCTCTAATGGAACAGTGTAATATCACTATGCCTCTTCTTTAAGACTGCCTATGTTTTAGTAAAAAGGCTGTGCTTCTTCTTCACTATTCTTCTCTGTTTTCTTTTCCCACCATTTTGTAAGAGCTTTGGTTATAGGAGGGTAAAACAAAACAGACCCTGAATCTTTAT

mRNA sequence

GAGGGAATGGACACTTGAGAAGAGATATGCTCTGCGGGCAAGGAGAATCTGACACTTAAACTTTAAACGTGGTTGTATATGGTTCATATTCAATGGTCATTTGGTCCATGCCCAATTTCTTTTCAAAAGAATAAAATAATGAGAGAAACTTTCGTTTATTCTTCATAAATCATTGGGATATCAAATGGGTAATTGCTAGAAATAGTTCTCCACATGTGACAAAGGCCACAGAGCTTCAATATATACCCACCTTCCCTTCAATCGCCATTCACATTTAAGTTTCTTCCGTATTCCCAGATTCAAACATTTCCCCACTACCCATTTCATACGAAAAATGGTCCTCGAAATTGCGGTCAAGGCTGCCACTGGTTCACCTGATGTGGTTGGGGACTGCCCTTTCTGCCATAGGGTTGTCTTAACTCTGGAGGAGAAGAAAGTTCCTTACAAATTGCTTCTCATCAATCTATCCGACAAACCCAGTTGGTTATTGGAAGTGAACCCAGAAGGGAAGGTGCCGGTGGCGAAGTTTGATGGCAAATGGGTTCCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTTGTTACTCCTCCTCAATTTTCTTCTGTAGGATCAAAGATGTTTGGTGCTTTTGTAAAATTCTTGTTAAGCAAGGACCCGAATGATGGTACAGAGCAGGCTTTCCTTGAAGAGTTGAAGGCGCTCAATGAGCATCTTAAGGCTCATGGTCCTTATATTGCTGGAGAGAAGGTAACTGCAGTTGATTTGAGTCTGGCACCAAAGTTGTTCCATGTTGATGTTGCTCTTGGCCATTTTAAGAAGTGGTCTATCCCCGAAGACTTGGCCAGTTTAATCGCCTACAAAGAGTTGCTTTTTGCTCGAGAATCTTTCGTGAAGACGAAAACTGCCCCGGAATATGTAATTGCTGGATGGGGGCCGAAGGTGAATCCTGCATGAATTGGGAGATGTTTTCATGTCAAGTTATGAAATAATTGGGGGATTTTAATCCTCTTAAGAATGTGGCTGTGGTTGCTTGCTATCTTCTTATGTGTCAATAAATAGTGTATTTTTCATATGTGTGCTCTAATGGAACAGTGTAATATCACTATGCCTCTTCTTTAAGACTGCCTATGTTTTAGTAAAAAGGCTGTGCTTCTTCTTCACTATTCTTCTCTGTTTTCTTTTCCCACCATTTTGTAAGAGCTTTGGTTATAGGAGGGTAAAACAAAACAGACCCTGAATCTTTAT

Coding sequence (CDS)

ATGGTCCTCGAAATTGCGGTCAAGGCTGCCACTGGTTCACCTGATGTGGTTGGGGACTGCCCTTTCTGCCATAGGGTTGTCTTAACTCTGGAGGAGAAGAAAGTTCCTTACAAATTGCTTCTCATCAATCTATCCGACAAACCCAGTTGGTTATTGGAAGTGAACCCAGAAGGGAAGGTGCCGGTGGCGAAGTTTGATGGCAAATGGGTTCCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTTGTTACTCCTCCTCAATTTTCTTCTGTAGGATCAAAGATGTTTGGTGCTTTTGTAAAATTCTTGTTAAGCAAGGACCCGAATGATGGTACAGAGCAGGCTTTCCTTGAAGAGTTGAAGGCGCTCAATGAGCATCTTAAGGCTCATGGTCCTTATATTGCTGGAGAGAAGGTAACTGCAGTTGATTTGAGTCTGGCACCAAAGTTGTTCCATGTTGATGTTGCTCTTGGCCATTTTAAGAAGTGGTCTATCCCCGAAGACTTGGCCAGTTTAATCGCCTACAAAGAGTTGCTTTTTGCTCGAGAATCTTTCGTGAAGACGAAAACTGCCCCGGAATATGTAATTGCTGGATGGGGGCCGAAGGTGAATCCTGCATGA

Protein sequence

MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKVPVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTEQAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLIAYKELLFARESFVKTKTAPEYVIAGWGPKVNPA

Homology

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BLAST of Lsi04G008540 vs. ExPASy Swiss-Prot
Match: Q9FRL8 (Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.8e-84
Identity = 145/212 (68.40%), Postives = 175/212 (82.55%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M L+I VK A G+PDV+GDCPF  RV+LTLEEKK+PYK  LIN+SDKP W L+++PEGKV
Sbjct: 1   MALDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV K DGKWV DSDVIVG+LEEKYPEPSL TPP+F+SVGSK+FGAFV FL SKD NDG+E
Sbjct: 61  PVVKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSE 120

Query: 121 QAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASL 180
           +A ++EL+AL  HLK H GP++AGEK+TAVDLSLAPKL+H++VALGH+K WS+PE L S+
Sbjct: 121 KALVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSV 180

Query: 181 IAYKELLFARESFVKTKTAPEYVIAGWGPKVN 212
             Y + LF+RESF  TK   E V+AGW  KVN
Sbjct: 181 RNYAKALFSRESFENTKAKKEIVVAGWESKVN 212

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BLAST of Lsi04G008540 vs. ExPASy Swiss-Prot
Match: Q65XA0 (Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 3.2e-81
Identity = 138/212 (65.09%), Postives = 172/212 (81.13%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAA G PD +GDCPF  RV+LTLEEKKVPY++ LI++ +KP W L+++PEGKV
Sbjct: 1   MGVEVCVKAAVGHPDTLGDCPFSQRVLLTLEEKKVPYEMKLIDVQNKPDWFLKISPEGKV 60

Query: 61  PVAK-FDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGT 120
           PV    DGKW+PDSDVI  ++EEKYP PSLVTPP+++SVGSK+F  F  FL SKDPNDG+
Sbjct: 61  PVFNGGDGKWIPDSDVITQVIEEKYPTPSLVTPPEYASVGSKIFSCFTTFLKSKDPNDGS 120

Query: 121 EQAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASL 180
           E+A L EL+AL EHLKAHGP+I G+ ++A DLSLAPKL+H+ VAL HFK W IPEDL ++
Sbjct: 121 EKALLTELQALEEHLKAHGPFINGQNISAADLSLAPKLYHLQVALEHFKGWKIPEDLTNV 180

Query: 181 IAYKELLFARESFVKTKTAPEYVIAGWGPKVN 212
            AY E LF+RESF+KTK A E++IAGW PKVN
Sbjct: 181 HAYTEALFSRESFIKTKAAKEHLIAGWAPKVN 212

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BLAST of Lsi04G008540 vs. ExPASy Swiss-Prot
Match: Q9FWR4 (Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 6.6e-79
Identity = 139/213 (65.26%), Postives = 167/213 (78.40%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M LEI VKAA G+PD +GDCPF  R +LTLEEK + YK+ LINLSDKP W L+++P+GKV
Sbjct: 1   MALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV K D KWV DSDVIVGILEEKYP+P L TP +F+SVGS +FG F  FL SKD NDG+E
Sbjct: 61  PVLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSE 120

Query: 121 QAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASL 180
            A L EL+AL  HLK+H GP+IAGE+V+AVDLSLAPKL+H+ VALGHFK WS+PE    +
Sbjct: 121 HALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHV 180

Query: 181 IAYKELLFARESFVKTKTAPEYVIAGWGPKVNP 213
             Y + LF+ +SF KTKT  +YVI+GW PKVNP
Sbjct: 181 HNYMKTLFSLDSFEKTKTEEKYVISGWAPKVNP 213

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BLAST of Lsi04G008540 vs. ExPASy Swiss-Prot
Match: Q8LE52 (Glutathione S-transferase DHAR3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DHAR3 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.4e-73
Identity = 124/208 (59.62%), Postives = 161/208 (77.40%), Query Frame = 0


Query: 3   LEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKVPV 62
           LEI VKA+  +P+ +GDCPFC +V+LT+EEK VPY + +++LS+KP W L+++PEGKVPV
Sbjct: 49  LEICVKASITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLSNKPEWFLKISPEGKVPV 108

Query: 63  AKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTEQA 122
            KFD KWVPDSDVI   LEEKYPEP L TPP+ +SVGSK+F  FV FL SKD  DGTEQ 
Sbjct: 109 VKFDEKWVPDSDVITQALEEKYPEPPLATPPEKASVGSKIFSTFVGFLKSKDSGDGTEQV 168

Query: 123 FLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLIAY 182
            L+EL   N+++K +GP+I GEK++A DLSLAPKL+H+ +ALGH+K WS+P+ L  + +Y
Sbjct: 169 LLDELTTFNDYIKDNGPFINGEKISAADLSLAPKLYHMKIALGHYKNWSVPDSLPFVKSY 228

Query: 183 KELLFARESFVKTKTAPEYVIAGWGPKV 211
            E +F+RESF  T+   E VIAGW PKV
Sbjct: 229 MENVFSRESFTNTRAETEDVIAGWRPKV 256

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BLAST of Lsi04G008540 vs. ExPASy Swiss-Prot
Match: Q9FG59 (Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR4 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.2e-73
Identity = 131/216 (60.65%), Postives = 172/216 (79.63%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAA+G+PDV+GDCPF  R++LTLE+KK+PYK  LI++S KP W L ++P+GK+
Sbjct: 1   MGIEVCVKAASGAPDVLGDCPFGQRILLTLEDKKLPYKTHLIDVSLKPDWFLAISPKGKL 60

Query: 61  PVAKF--DGKWVPDSDVIVGILEEKYPEPSLVT-PPQFSSVGSKMFGAFVKFLLSKD-PN 120
           P+ KF  D  WV DSD+IVGI+EEKYPEPSLVT PP+F+SVGSK+ GAFV FL SKD  N
Sbjct: 61  PLVKFDEDENWVADSDLIVGIIEEKYPEPSLVTFPPEFASVGSKIIGAFVMFLTSKDHAN 120

Query: 121 DGTEQAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPED 180
           DG++ A L+EL+AL+ HLK H GP++AG+KVT VDLSLAPKL+H++  LGHF  W +PE 
Sbjct: 121 DGSDMALLDELEALDHHLKTHVGPFVAGDKVTVVDLSLAPKLYHLETTLGHFMDWCVPES 180

Query: 181 LASLIAYKELLFARESFVKTKTAPEYVIAGWGPKVN 212
           L ++  Y ++LF+ ESF KTK A EY+IA W PK++
Sbjct: 181 LTNVRDYMKVLFSLESFEKTKAAKEYLIASWAPKLD 216

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BLAST of Lsi04G008540 vs. ExPASy TrEMBL
Match: A0A6J1IFU4 (glutathione S-transferase DHAR2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476861 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.5e-102
Identity = 178/213 (83.57%), Postives = 197/213 (92.49%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAATG+PDV+GDCPFCHRV+LTLEEKKV YKLLL+NLSDKPSW LEV+PEGKV
Sbjct: 1   MAIEVVVKAATGAPDVLGDCPFCHRVLLTLEEKKVAYKLLLVNLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFDGKWVPDSDVIVGILEEKYP+PSLVTPPQFSSVGSK+ GAFVKFLLSKDP DG+E
Sbjct: 61  PVVKFDGKWVPDSDVIVGILEEKYPDPSLVTPPQFSSVGSKINGAFVKFLLSKDPTDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLI 180
           QA LEELKAL+EHLKAHGPY+AGEKVTAVDLSLAPKL+H+D+ALGHFKKW  PE+L+SL 
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYHIDIALGHFKKWVFPENLSSLS 180

Query: 181 AYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           AYK+LLFARESFVKTK  PE+VIAGW  KVN A
Sbjct: 181 AYKKLLFARESFVKTKAVPEHVIAGWETKVNAA 213

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BLAST of Lsi04G008540 vs. ExPASy TrEMBL
Match: A0A6J1GWG8 (glutathione S-transferase DHAR2-like OS=Cucurbita moschata OX=3662 GN=LOC111458209 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.2e-101
Identity = 177/213 (83.10%), Postives = 197/213 (92.49%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAATG+PDV+GDCPFCHRV+LTLEEKKV YKLLL+NLSDKPSW LEV+PEGKV
Sbjct: 1   MAIEVVVKAATGAPDVLGDCPFCHRVLLTLEEKKVAYKLLLVNLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFDGKWVPDSDVIVGILE+KYP+PSLVTPPQFSSVGSK+ GAFVKFLLSKDP+DG+E
Sbjct: 61  PVVKFDGKWVPDSDVIVGILEDKYPDPSLVTPPQFSSVGSKINGAFVKFLLSKDPSDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLI 180
           QA LEELKAL+EHLKAHGPY+AGEKVTAVDLSLAPKL+H+D+ALGHFKKW  PE+L SL 
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYHIDIALGHFKKWVFPENLRSLS 180

Query: 181 AYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           AYK+LLFARESFVKTK  PE+VIAGW  KVN A
Sbjct: 181 AYKKLLFARESFVKTKAVPEHVIAGWETKVNAA 213

[Close]

BLAST of Lsi04G008540 vs. ExPASy TrEMBL
Match: A0A6J1IGZ9 (glutathione S-transferase DHAR2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476861 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.3e-98
Identity = 178/232 (76.72%), Postives = 197/232 (84.91%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAATG+PDV+GDCPFCHRV+LTLEEKKV YKLLL+NLSDKPSW LEV+PEGKV
Sbjct: 1   MAIEVVVKAATGAPDVLGDCPFCHRVLLTLEEKKVAYKLLLVNLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFDGKWVPDSDVIVGILEEKYP+PSLVTPPQFSSVGSK+ GAFVKFLLSKDP DG+E
Sbjct: 61  PVVKFDGKWVPDSDVIVGILEEKYPDPSLVTPPQFSSVGSKINGAFVKFLLSKDPTDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAG-------------------EKVTAVDLSLAPKLFHVD 180
           QA LEELKAL+EHLKAHGPY+AG                   EKVTAVDLSLAPKL+H+D
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYHIDIEKVTAVDLSLAPKLYHID 180

Query: 181 VALGHFKKWSIPEDLASLIAYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           +ALGHFKKW  PE+L+SL AYK+LLFARESFVKTK  PE+VIAGW  KVN A
Sbjct: 181 IALGHFKKWVFPENLSSLSAYKKLLFARESFVKTKAVPEHVIAGWETKVNAA 232

[Close]

BLAST of Lsi04G008540 vs. ExPASy TrEMBL
Match: A0A6J1CFE5 (glutathione S-transferase DHAR2-like OS=Momordica charantia OX=3673 GN=LOC111010278 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.0e-98
Identity = 175/213 (82.16%), Postives = 193/213 (90.61%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M LE+A KAA G+PDV+GDCPF  RV+LTLEEKK+PYKL LINLSDKPSW LEV+PEGKV
Sbjct: 1   MDLEVAAKAAVGAPDVIGDCPFTQRVLLTLEEKKIPYKLHLINLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFD KWVPDSDVIVGILEEKYPEPSLVTPP+FSSVGSK+ GAFVKFL+SKD NDG+E
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLMSKDSNDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLI 180
           QA LEELKAL+EHLKAHGPY+AGEKVTA DLSLAPKL+ +DVALGHFKKW+ PE+LASL 
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGEKVTAADLSLAPKLYQIDVALGHFKKWTFPENLASLS 180

Query: 181 AYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           AYK+LLFARESFVKTK APE+VIAGW  KVN A
Sbjct: 181 AYKKLLFARESFVKTKAAPEHVIAGWALKVNAA 213

[Close]

BLAST of Lsi04G008540 vs. ExPASy TrEMBL
Match: A0A5A7V955 (Glutathione S-transferase DHAR2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00170 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 5.6e-97
Identity = 176/214 (82.24%), Postives = 192/214 (89.72%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E AVKAA G+PD +GDCPF  RV+LTLEEKKVPYKL LINLSDKPSW L+V+PEGKV
Sbjct: 1   MAIEAAVKAAVGAPDEIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPSWFLKVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFD KWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSK+F AF KFL SKDP D +E
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKIFSAFGKFLKSKDPKDHSE 120

Query: 121 QAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASL 180
           +  L ELKAL+EHLKAH GPY+AGEKVTAVDLSLAPKL+HVD+ALGHFKKWSIP++LASL
Sbjct: 121 EDLLVELKALDEHLKAHDGPYVAGEKVTAVDLSLAPKLYHVDIALGHFKKWSIPKELASL 180

Query: 181 IAYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           IAYKELLFARESFVKTK  PE+VIAGW PKVNPA
Sbjct: 181 IAYKELLFARESFVKTKATPEHVIAGWEPKVNPA 214

[Close]

BLAST of Lsi04G008540 vs. NCBI nr
Match: XP_038892889.1 (glutathione S-transferase DHAR2 [Benincasa hispida])

HSP 1 Score: 386.0 bits (990), Expect = 2.2e-103
Identity = 182/213 (85.45%), Postives = 202/213 (94.84%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           MVLE+AVKAATG+PDV+GDCPFC RV+LTLE KKVPYKLLL+NLSDKPSWLLEV+PEGKV
Sbjct: 1   MVLELAVKAATGAPDVLGDCPFCQRVILTLEAKKVPYKLLLVNLSDKPSWLLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           P+AKFD KWVPDSDVIVGILEEKYPEPSLVTPP+FSSVGSK+FGAFVKFL SKDPNDG+E
Sbjct: 61  PLAKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKLFGAFVKFLKSKDPNDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLI 180
           QAFLEELKAL++HLKA GPY+AGEKVTAVDLSLAPKL+H+DV L HFKKWSIP+DLASLI
Sbjct: 121 QAFLEELKALDDHLKARGPYVAGEKVTAVDLSLAPKLYHIDVTLDHFKKWSIPKDLASLI 180

Query: 181 AYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           +YKELLFA+ESFVKTK AP+YVIAGW  K+N A
Sbjct: 181 SYKELLFAQESFVKTKAAPKYVIAGWERKLNSA 213

[Close]

BLAST of Lsi04G008540 vs. NCBI nr
Match: XP_022976477.1 (glutathione S-transferase DHAR2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 379.8 bits (974), Expect = 1.6e-101
Identity = 178/213 (83.57%), Postives = 197/213 (92.49%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAATG+PDV+GDCPFCHRV+LTLEEKKV YKLLL+NLSDKPSW LEV+PEGKV
Sbjct: 1   MAIEVVVKAATGAPDVLGDCPFCHRVLLTLEEKKVAYKLLLVNLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFDGKWVPDSDVIVGILEEKYP+PSLVTPPQFSSVGSK+ GAFVKFLLSKDP DG+E
Sbjct: 61  PVVKFDGKWVPDSDVIVGILEEKYPDPSLVTPPQFSSVGSKINGAFVKFLLSKDPTDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLI 180
           QA LEELKAL+EHLKAHGPY+AGEKVTAVDLSLAPKL+H+D+ALGHFKKW  PE+L+SL 
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYHIDIALGHFKKWVFPENLSSLS 180

Query: 181 AYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           AYK+LLFARESFVKTK  PE+VIAGW  KVN A
Sbjct: 181 AYKKLLFARESFVKTKAVPEHVIAGWETKVNAA 213

[Close]

BLAST of Lsi04G008540 vs. NCBI nr
Match: XP_022956487.1 (glutathione S-transferase DHAR2-like [Cucurbita moschata] >KAG7031631.1 Glutathione S-transferase DHAR2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 378.3 bits (970), Expect = 4.5e-101
Identity = 177/213 (83.10%), Postives = 197/213 (92.49%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAATG+PDV+GDCPFCHRV+LTLEEKKV YKLLL+NLSDKPSW LEV+PEGKV
Sbjct: 1   MAIEVVVKAATGAPDVLGDCPFCHRVLLTLEEKKVAYKLLLVNLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFDGKWVPDSDVIVGILE+KYP+PSLVTPPQFSSVGSK+ GAFVKFLLSKDP+DG+E
Sbjct: 61  PVVKFDGKWVPDSDVIVGILEDKYPDPSLVTPPQFSSVGSKINGAFVKFLLSKDPSDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLI 180
           QA LEELKAL+EHLKAHGPY+AGEKVTAVDLSLAPKL+H+D+ALGHFKKW  PE+L SL 
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYHIDIALGHFKKWVFPENLRSLS 180

Query: 181 AYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           AYK+LLFARESFVKTK  PE+VIAGW  KVN A
Sbjct: 181 AYKKLLFARESFVKTKAVPEHVIAGWETKVNAA 213

[Close]

BLAST of Lsi04G008540 vs. NCBI nr
Match: XP_023526522.1 (glutathione S-transferase DHAR2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.1 bits (967), Expect = 1.0e-100
Identity = 176/213 (82.63%), Postives = 197/213 (92.49%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAATG+PDV+GDCPFCHRV+LTLEEKKV YKLLL+NLSDKPSW LEV+PEGKV
Sbjct: 1   MAIEVVVKAATGAPDVLGDCPFCHRVLLTLEEKKVAYKLLLVNLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFDGKWVPDSDVIVGILE+KYP+PSLVTPPQFSSVGSK+ GAFVKFLLSKDP+DG+E
Sbjct: 61  PVVKFDGKWVPDSDVIVGILEDKYPDPSLVTPPQFSSVGSKINGAFVKFLLSKDPSDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLI 180
           QA LEELKAL+EHLKAHGPY+AG+KVTAVDLSLAPKL+H+D+ALGHFKKW  PE+L SL 
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGQKVTAVDLSLAPKLYHIDIALGHFKKWVFPENLRSLS 180

Query: 181 AYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           AYK+LLFARESFVKTK  PE+VIAGW  KVN A
Sbjct: 181 AYKKLLFARESFVKTKAVPEHVIAGWETKVNAA 213

[Close]

BLAST of Lsi04G008540 vs. NCBI nr
Match: XP_022976471.1 (glutathione S-transferase DHAR2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 368.2 bits (944), Expect = 4.7e-98
Identity = 178/232 (76.72%), Postives = 197/232 (84.91%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M +E+ VKAATG+PDV+GDCPFCHRV+LTLEEKKV YKLLL+NLSDKPSW LEV+PEGKV
Sbjct: 1   MAIEVVVKAATGAPDVLGDCPFCHRVLLTLEEKKVAYKLLLVNLSDKPSWFLEVSPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV KFDGKWVPDSDVIVGILEEKYP+PSLVTPPQFSSVGSK+ GAFVKFLLSKDP DG+E
Sbjct: 61  PVVKFDGKWVPDSDVIVGILEEKYPDPSLVTPPQFSSVGSKINGAFVKFLLSKDPTDGSE 120

Query: 121 QAFLEELKALNEHLKAHGPYIAG-------------------EKVTAVDLSLAPKLFHVD 180
           QA LEELKAL+EHLKAHGPY+AG                   EKVTAVDLSLAPKL+H+D
Sbjct: 121 QALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYHIDIEKVTAVDLSLAPKLYHID 180

Query: 181 VALGHFKKWSIPEDLASLIAYKELLFARESFVKTKTAPEYVIAGWGPKVNPA 214
           +ALGHFKKW  PE+L+SL AYK+LLFARESFVKTK  PE+VIAGW  KVN A
Sbjct: 181 IALGHFKKWVFPENLSSLSAYKKLLFARESFVKTKAVPEHVIAGWETKVNAA 232

[Close]

BLAST of Lsi04G008540 vs. TAIR 10
Match: AT1G75270.1 (dehydroascorbate reductase 2 )

HSP 1 Score: 313.5 bits (802), Expect = 1.3e-85
Identity = 145/212 (68.40%), Postives = 175/212 (82.55%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M L+I VK A G+PDV+GDCPF  RV+LTLEEKK+PYK  LIN+SDKP W L+++PEGKV
Sbjct: 1   MALDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV K DGKWV DSDVIVG+LEEKYPEPSL TPP+F+SVGSK+FGAFV FL SKD NDG+E
Sbjct: 61  PVVKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSE 120

Query: 121 QAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASL 180
           +A ++EL+AL  HLK H GP++AGEK+TAVDLSLAPKL+H++VALGH+K WS+PE L S+
Sbjct: 121 KALVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSV 180

Query: 181 IAYKELLFARESFVKTKTAPEYVIAGWGPKVN 212
             Y + LF+RESF  TK   E V+AGW  KVN
Sbjct: 181 RNYAKALFSRESFENTKAKKEIVVAGWESKVN 212

[Close]

BLAST of Lsi04G008540 vs. TAIR 10
Match: AT1G19570.1 (dehydroascorbate reductase )

HSP 1 Score: 295.0 bits (754), Expect = 4.7e-80
Identity = 139/213 (65.26%), Postives = 167/213 (78.40%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M LEI VKAA G+PD +GDCPF  R +LTLEEK + YK+ LINLSDKP W L+++P+GKV
Sbjct: 1   MALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV K D KWV DSDVIVGILEEKYP+P L TP +F+SVGS +FG F  FL SKD NDG+E
Sbjct: 61  PVLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSE 120

Query: 121 QAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASL 180
            A L EL+AL  HLK+H GP+IAGE+V+AVDLSLAPKL+H+ VALGHFK WS+PE    +
Sbjct: 121 HALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHV 180

Query: 181 IAYKELLFARESFVKTKTAPEYVIAGWGPKVNP 213
             Y + LF+ +SF KTKT  +YVI+GW PKVNP
Sbjct: 181 HNYMKTLFSLDSFEKTKTEEKYVISGWAPKVNP 213

[Close]

BLAST of Lsi04G008540 vs. TAIR 10
Match: AT1G19570.2 (dehydroascorbate reductase )

HSP 1 Score: 286.6 bits (732), Expect = 1.7e-77
Identity = 138/213 (64.79%), Postives = 167/213 (78.40%), Query Frame = 0


Query: 1   MVLEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKV 60
           M LEI VKAA G+PD +GDCPF  R +LTLEEK + YK+ LINLSDKP + L+++P+GKV
Sbjct: 1   MALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQF-LDISPQGKV 60

Query: 61  PVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTE 120
           PV K D KWV DSDVIVGILEEKYP+P L TP +F+SVGS +FG F  FL SKD NDG+E
Sbjct: 61  PVLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSE 120

Query: 121 QAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASL 180
            A L EL+AL  HLK+H GP+IAGE+V+AVDLSLAPKL+H+ VALGHFK WS+PE    +
Sbjct: 121 HALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHV 180

Query: 181 IAYKELLFARESFVKTKTAPEYVIAGWGPKVNP 213
             Y + LF+ +SF KTKT  +YVI+GW PKVNP
Sbjct: 181 HNYMKTLFSLDSFEKTKTEEKYVISGWAPKVNP 212

[Close]

BLAST of Lsi04G008540 vs. TAIR 10
Match: AT5G16710.1 (dehydroascorbate reductase 1 )

HSP 1 Score: 277.3 bits (708), Expect = 1.0e-74
Identity = 124/208 (59.62%), Postives = 161/208 (77.40%), Query Frame = 0


Query: 3   LEIAVKAATGSPDVVGDCPFCHRVVLTLEEKKVPYKLLLINLSDKPSWLLEVNPEGKVPV 62
           LEI VKA+  +P+ +GDCPFC +V+LT+EEK VPY + +++LS+KP W L+++PEGKVPV
Sbjct: 49  LEICVKASITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLSNKPEWFLKISPEGKVPV 108

Query: 63  AKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFSSVGSKMFGAFVKFLLSKDPNDGTEQA 122
            KFD KWVPDSDVI   LEEKYPEP L TPP+ +SVGSK+F  FV FL SKD  DGTEQ 
Sbjct: 109 VKFDEKWVPDSDVITQALEEKYPEPPLATPPEKASVGSKIFSTFVGFLKSKDSGDGTEQV 168

Query: 123 FLEELKALNEHLKAHGPYIAGEKVTAVDLSLAPKLFHVDVALGHFKKWSIPEDLASLIAY 182
            L+EL   N+++K +GP+I GEK++A DLSLAPKL+H+ +ALGH+K WS+P+ L  + +Y
Sbjct: 169 LLDELTTFNDYIKDNGPFINGEKISAADLSLAPKLYHMKIALGHYKNWSVPDSLPFVKSY 228

Query: 183 KELLFARESFVKTKTAPEYVIAGWGPKV 211
            E +F+RESF  T+   E VIAGW PKV
Sbjct: 229 MENVFSRESFTNTRAETEDVIAGWRPKV 256

[Close]

BLAST of Lsi04G008540 vs. TAIR 10
Match: AT1G19550.1 (Glutathione S-transferase family protein )

HSP 1 Score: 176.8 bits (447), Expect = 1.9e-44
Identity = 89/177 (50.28%), Postives = 115/177 (64.97%), Query Frame = 0


Query: 39  LLLINLSDKPS--WLLEVNPEGKVPVAKFDGKWVPDSDVIVGILEEKYPEPSLVTPPQFS 98
           +L++ +   PS     +++P+GKVPV K D KWV DSD  VGILEEKYP+P L TP +F+
Sbjct: 1   MLVVQICSSPSHEMFWDISPQGKVPVLKIDDKWVTDSDATVGILEEKYPDPPLKTPAEFA 60

Query: 99  SVGSKMFGAFVKFLLSKDPNDGTEQAFLEELKALNEHLKAH-GPYIAGEKVTAVDLSLAP 158
           SVGS +F                        +AL  HLK+H GP+IAGE+V+AVDLSLAP
Sbjct: 61  SVGSNIF------------------------EALENHLKSHDGPFIAGERVSAVDLSLAP 120

Query: 159 KLFHVDVALGHFKKWSIPEDLASLIAYKELLFARESFVKTKTAPEYVIAGWGPKVNP 213
           KL+H+ VALGHFK WS+PE    +  Y + LF+ +SF KTKT  + VI+GW PKVNP
Sbjct: 121 KLYHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKCVISGWAPKVNP 153

The following BLAST results are available for this feature:

BLAST of Lsi04G008540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FRL8	1.8e-84	68.40	Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV...	[more]
Q65XA0	3.2e-81	65.09	Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japon...	[more]
Q9FWR4	6.6e-79	65.26	Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 G...	[more]
Q8LE52	1.4e-73	59.62	Glutathione S-transferase DHAR3, chloroplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9FG59	3.2e-73	60.65	Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR...	[more]

BLAST of Lsi04G008540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IFU4	7.5e-102	83.57	glutathione S-transferase DHAR2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GWG8	2.2e-101	83.10	glutathione S-transferase DHAR2-like OS=Cucurbita moschata OX=3662 GN=LOC1114582...	[more]
A0A6J1IGZ9	2.3e-98	76.72	glutathione S-transferase DHAR2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1CFE5	5.0e-98	82.16	glutathione S-transferase DHAR2-like OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A5A7V955	5.6e-97	82.24	Glutathione S-transferase DHAR2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]

BLAST of Lsi04G008540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892889.1	2.2e-103	85.45	glutathione S-transferase DHAR2 [Benincasa hispida]	[more]
XP_022976477.1	1.6e-101	83.57	glutathione S-transferase DHAR2-like isoform X2 [Cucurbita maxima]	[more]
XP_022956487.1	4.5e-101	83.10	glutathione S-transferase DHAR2-like [Cucurbita moschata] >KAG7031631.1 Glutathi...	[more]
XP_023526522.1	1.0e-100	82.63	glutathione S-transferase DHAR2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022976471.1	4.7e-98	76.72	glutathione S-transferase DHAR2-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Lsi04G008540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75270.1	1.3e-85	68.40	dehydroascorbate reductase 2	[more]
AT1G19570.1	4.7e-80	65.26	dehydroascorbate reductase	[more]
AT1G19570.2	1.7e-77	64.79	dehydroascorbate reductase	[more]
AT5G16710.1	1.0e-74	59.62	dehydroascorbate reductase 1	[more]
AT1G19550.1	1.9e-44	50.28	Glutathione S-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..89 e-value: 3.7E-29 score: 102.6
None	No IPR available	PFAM	PF13410	GST_C_2	coord: 120..176 e-value: 4.0E-8 score: 33.2
None	No IPR available	GENE3D	1.20.1050.10		coord: 90..212 e-value: 4.5E-45 score: 154.6
None	No IPR available	SFLD	SFLDG00358	Main_(cytGST)	coord: 19..166 e-value: 2.2E-25 score: 84.3
None	No IPR available	PANTHER	PTHR44420:SF2	GLUTATHIONE S-TRANSFERASE DHAR2-RELATED	coord: 1..211
None	No IPR available	CDD	cd00570	GST_N_family	coord: 20..77 e-value: 2.39618E-14 score: 63.3596
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF13417	GST_N_3	coord: 19..87 e-value: 4.6E-16 score: 58.9
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 10..88 score: 14.488197
IPR040079	Glutathione Transferase family	SFLD	SFLDS00019	Glutathione_Transferase_(cyto	coord: 19..166 e-value: 2.2E-25 score: 84.3
IPR044627	Dehydroascorbate reductases DHAR1/2/3/4	PANTHER	PTHR44420	GLUTATHIONE S-TRANSFERASE DHAR2-RELATED	coord: 1..211
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 19..87
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 84..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G008540.1	Lsi04G008540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0033355	ascorbate glutathione cycle
biological_process	GO:0098869	cellular oxidant detoxification
biological_process	GO:0010731	protein glutathionylation
biological_process	GO:0006749	glutathione metabolic process
molecular_function	GO:0045174	glutathione dehydrogenase (ascorbate) activity
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0005515	protein binding