Lsi04G008320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G008320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiontranscription termination factor MTEF18, mitochondrial-like
Locationchr04: 8028949 .. 8030700 (+)
RNA-Seq ExpressionLsi04G008320
SyntenyLsi04G008320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTCTTCTTCGATTATTGCTGATAACAAGTTCAACTTAGTTCGAGTCTTGTACTGGCGAATTGGGTCTTCCTCTGTTGCCTCTAACGCTAGATTTTATCGAACTAAAAAGGCTCCCCAAACGGAAGAATGTGAAAATTCTGGGGAAATTTTGACTAGGGTTAGCAGAAACGGTCATCGGATTTCTCGGGCCATTAGAAAGGAGGCTCAGGCTGCGTTGTTGGAGTATTTGCATTCTACTAGAGGGATTCAACATGTGGATGCTGAGAATATGAGTAGAAATTCACCAATCTTTCTTGAGGAACTTCTGGGAAGAGTTGAACATGAGGGCGATATTGCGCGGTCAATTACGCGATTTTTGCGCTACCATCCGATTAATGAATTCGAGCCCTTCTTTGAGAGTGTGGGCTTGCAACCTGCAGAGTATAATGCTTTTCTTCCACGTAATTTGATGTTTTTGAGTGATGATGATTTATTGCTTGAGAACTATCATGTGTTGCGTAACTATGGAATTCAACGAAATAAGATAGGGAAAATATATAAGGAAGCAACTCAGATATTTCAGTATGACTATGGTGTTTTGCTGTCGAAATTAAAAGCATATGAAGAACTTGGTCTCAGCCAAGCTACTGTTGTTAAATTTGTTGTTTGTAGCCCCTATCTATTGGTTGGTGGTGTTAATGATGGGTTTGCCCAGGTATTAGATAAATTGAAGAACATTGGATTTGAATCAAGCTGGGTTGAAGAACAACTGATTGATGGTAATTCCTATAACTGGAAGCAAATTCTTGGGTTGCTTTTCTGGTTTAAACAGATGGGTTGCTGTAATGAGAAGTTGGCTGATTTAATCAGCCAACATCCAGATTTTTTATTTGAGGATTCAGGAAGTAAATCACTTTCCTTAATTGGGTTATTACTGAAAATGGGATCCTCAATGATCCAGATATGTTCCGTGTTCCTGCAATTCCCTCAAATCCGTGTTGGTAAGTTTGTTTCAAATATGAGGCTATGTCTTTTGTTCTTCAATGAAATAGACATGGGTGTGCAAGAGATAGGATACCTTTTCAGATCTCATCCCTTGTTATTGGGATCGTGTACTCTGAAAAAGACTAACAGCTTGCTCGCTAACTTGAATGTTGGGAAGAAACGACTTTGTCAATTCATCTTAGAGAATCCAGAAGAATTGAAGAACTGGAAGCTCGGGACAAGAGTTCCACCTCTACCAGACACAGGAGAGGTTATAAGATCAAAGCAACAGAAAACTCAGTTCTTGTTAAAATTAGGACTTGAAGAAAACTCGAGTGAGATGAAAAAAGCACTCAAGGTGTTTCGAGGTAAGGGAGCAGAGCTCCAAGAGCGGTTCGATTGTATTGTGGAAGCTGGCATTGATGAGAAGGCTGTTTACAAAATGATTTTAGTTTCTCCACAAATCCTTAACCAAACAAAAGATATAATAGAGGAAAAGATAGATTTTCTTGTAAATAATTTGGGGTATCCTGTTTCATCACTAGTAAATTTCCCTTCATATCTTTCCTATACAACTCAGAGGGTCACTCTTAGATTCTCAATGTATAGTTGGCTCAAGGAACAAGGAACAGCTGACCCGATGTTGGCATTGAGCACTATTGTTGCGTGTTCAGAAAATACATTTCTAAGACAGTATGTGAATCATCATCCCAGAGGCATGGAAGTTTGGCAGAATTTGAAGAGAGAAATATATTCAGATTCTATTGCGTCTCCAGCTCATTAG

mRNA sequence

ATGGTTTCTTCTTCGATTATTGCTGATAACAAGTTCAACTTAGTTCGAGTCTTGTACTGGCGAATTGGGTCTTCCTCTGTTGCCTCTAACGCTAGATTTTATCGAACTAAAAAGGCTCCCCAAACGGAAGAATGTGAAAATTCTGGGGAAATTTTGACTAGGGTTAGCAGAAACGGTCATCGGATTTCTCGGGCCATTAGAAAGGAGGCTCAGGCTGCGTTGTTGGAGTATTTGCATTCTACTAGAGGGATTCAACATGTGGATGCTGAGAATATGAGTAGAAATTCACCAATCTTTCTTGAGGAACTTCTGGGAAGAGTTGAACATGAGGGCGATATTGCGCGGTCAATTACGCGATTTTTGCGCTACCATCCGATTAATGAATTCGAGCCCTTCTTTGAGAGTGTGGGCTTGCAACCTGCAGAGTATAATGCTTTTCTTCCACGTAATTTGATGTTTTTGAGTGATGATGATTTATTGCTTGAGAACTATCATGTGTTGCGTAACTATGGAATTCAACGAAATAAGATAGGGAAAATATATAAGGAAGCAACTCAGATATTTCAGTATGACTATGGTGTTTTGCTGTCGAAATTAAAAGCATATGAAGAACTTGGTCTCAGCCAAGCTACTGTTGTTAAATTTGTTGTTTGTAGCCCCTATCTATTGGTTGGTGGTGTTAATGATGGGTTTGCCCAGGTATTAGATAAATTGAAGAACATTGGATTTGAATCAAGCTGGGTTGAAGAACAACTGATTGATGGTAATTCCTATAACTGGAAGCAAATTCTTGGGTTGCTTTTCTGGTTTAAACAGATGGGTTGCTGTAATGAGAAGTTGGCTGATTTAATCAGCCAACATCCAGATTTTTTATTTGAGGATTCAGGAAGTAAATCACTTTCCTTAATTGGGTTATTACTGAAAATGGGATCCTCAATGATCCAGATATGTTCCGTGTTCCTGCAATTCCCTCAAATCCGTGTTGGTAAGTTTGTTTCAAATATGAGGCTATGTCTTTTGTTCTTCAATGAAATAGACATGGGTGTGCAAGAGATAGGATACCTTTTCAGATCTCATCCCTTGTTATTGGGATCGTGTACTCTGAAAAAGACTAACAGCTTGCTCGCTAACTTGAATGTTGGGAAGAAACGACTTTGTCAATTCATCTTAGAGAATCCAGAAGAATTGAAGAACTGGAAGCTCGGGACAAGAGTTCCACCTCTACCAGACACAGGAGAGGTTATAAGATCAAAGCAACAGAAAACTCAGTTCTTGTTAAAATTAGGACTTGAAGAAAACTCGAGTGAGATGAAAAAAGCACTCAAGGTGTTTCGAGGTAAGGGAGCAGAGCTCCAAGAGCGGTTCGATTGTATTGTGGAAGCTGGCATTGATGAGAAGGCTGTTTACAAAATGATTTTAGTTTCTCCACAAATCCTTAACCAAACAAAAGATATAATAGAGGAAAAGATAGATTTTCTTGTAAATAATTTGGGGTATCCTGTTTCATCACTAGTAAATTTCCCTTCATATCTTTCCTATACAACTCAGAGGGTCACTCTTAGATTCTCAATGTATAGTTGGCTCAAGGAACAAGGAACAGCTGACCCGATGTTGGCATTGAGCACTATTGTTGCGTGTTCAGAAAATACATTTCTAAGACAGTATGTGAATCATCATCCCAGAGGCATGGAAGTTTGGCAGAATTTGAAGAGAGAAATATATTCAGATTCTATTGCGTCTCCAGCTCATTAG

Coding sequence (CDS)

ATGGTTTCTTCTTCGATTATTGCTGATAACAAGTTCAACTTAGTTCGAGTCTTGTACTGGCGAATTGGGTCTTCCTCTGTTGCCTCTAACGCTAGATTTTATCGAACTAAAAAGGCTCCCCAAACGGAAGAATGTGAAAATTCTGGGGAAATTTTGACTAGGGTTAGCAGAAACGGTCATCGGATTTCTCGGGCCATTAGAAAGGAGGCTCAGGCTGCGTTGTTGGAGTATTTGCATTCTACTAGAGGGATTCAACATGTGGATGCTGAGAATATGAGTAGAAATTCACCAATCTTTCTTGAGGAACTTCTGGGAAGAGTTGAACATGAGGGCGATATTGCGCGGTCAATTACGCGATTTTTGCGCTACCATCCGATTAATGAATTCGAGCCCTTCTTTGAGAGTGTGGGCTTGCAACCTGCAGAGTATAATGCTTTTCTTCCACGTAATTTGATGTTTTTGAGTGATGATGATTTATTGCTTGAGAACTATCATGTGTTGCGTAACTATGGAATTCAACGAAATAAGATAGGGAAAATATATAAGGAAGCAACTCAGATATTTCAGTATGACTATGGTGTTTTGCTGTCGAAATTAAAAGCATATGAAGAACTTGGTCTCAGCCAAGCTACTGTTGTTAAATTTGTTGTTTGTAGCCCCTATCTATTGGTTGGTGGTGTTAATGATGGGTTTGCCCAGGTATTAGATAAATTGAAGAACATTGGATTTGAATCAAGCTGGGTTGAAGAACAACTGATTGATGGTAATTCCTATAACTGGAAGCAAATTCTTGGGTTGCTTTTCTGGTTTAAACAGATGGGTTGCTGTAATGAGAAGTTGGCTGATTTAATCAGCCAACATCCAGATTTTTTATTTGAGGATTCAGGAAGTAAATCACTTTCCTTAATTGGGTTATTACTGAAAATGGGATCCTCAATGATCCAGATATGTTCCGTGTTCCTGCAATTCCCTCAAATCCGTGTTGGTAAGTTTGTTTCAAATATGAGGCTATGTCTTTTGTTCTTCAATGAAATAGACATGGGTGTGCAAGAGATAGGATACCTTTTCAGATCTCATCCCTTGTTATTGGGATCGTGTACTCTGAAAAAGACTAACAGCTTGCTCGCTAACTTGAATGTTGGGAAGAAACGACTTTGTCAATTCATCTTAGAGAATCCAGAAGAATTGAAGAACTGGAAGCTCGGGACAAGAGTTCCACCTCTACCAGACACAGGAGAGGTTATAAGATCAAAGCAACAGAAAACTCAGTTCTTGTTAAAATTAGGACTTGAAGAAAACTCGAGTGAGATGAAAAAAGCACTCAAGGTGTTTCGAGGTAAGGGAGCAGAGCTCCAAGAGCGGTTCGATTGTATTGTGGAAGCTGGCATTGATGAGAAGGCTGTTTACAAAATGATTTTAGTTTCTCCACAAATCCTTAACCAAACAAAAGATATAATAGAGGAAAAGATAGATTTTCTTGTAAATAATTTGGGGTATCCTGTTTCATCACTAGTAAATTTCCCTTCATATCTTTCCTATACAACTCAGAGGGTCACTCTTAGATTCTCAATGTATAGTTGGCTCAAGGAACAAGGAACAGCTGACCCGATGTTGGCATTGAGCACTATTGTTGCGTGTTCAGAAAATACATTTCTAAGACAGTATGTGAATCATCATCCCAGAGGCATGGAAGTTTGGCAGAATTTGAAGAGAGAAATATATTCAGATTCTATTGCGTCTCCAGCTCATTAG

Protein sequence

MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGHRISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRFLRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKIYKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKNIGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSLSLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHPLLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQKTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILNQTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLALSTIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPAH
Homology
BLAST of Lsi04G008320 vs. ExPASy Swiss-Prot
Match: Q9M219 (Transcription termination factor MTEF18, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF18 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.5e-51
Identity = 147/517 (28.43%), Postives = 260/517 (50.29%), Query Frame = 0

Query: 69  EAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHE-GDIARSITRFLRYHPIN 128
           +AQ A+ +YLH+TR + +  AE ++ N+ + +  L+ +++      ++S+ + L YHPIN
Sbjct: 36  KAQQAITDYLHTTRSLSYTHAEQIASNASVSIRNLILKLDFSVPTFSKSLRKHLSYHPIN 95

Query: 129 EFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKIYKEATQI 188
           EFE FFES+G+  +E + FLP    F S+D  +L+    L  +G   NK+GK+YKE   +
Sbjct: 96  EFEFFFESIGIDYSEVSEFLPEKKFFFSEDRTVLDAAFALSGFGFPWNKLGKLYKEERLV 155

Query: 189 FQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGG--VNDGFAQVLDKLKNI--GF 248
           F    G + S+L  ++++G S   V+   +  P  L GG  +      +  KLK +   F
Sbjct: 156 FVQRPGEIESRLLKFKDIGFSTVAVIGTCLAIPRTLCGGGELGSEIRCLFVKLKRLFDEF 215

Query: 249 ESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSLSLI 308
           +S  + E+ +D    +W  +   +  F  +GC NE++ +L+ ++     E S    ++  
Sbjct: 216 DSHHLFEENVD----SWLAVSRKIRIFYDLGCENEEMWELMCRNKSLFLEYSEEALMNKA 275

Query: 309 GLLLKMGSSMIQICSVFLQFPQIR---VGKFVSNMRLCLLFF----NEIDMGVQEIGYLF 368
           G   + G S      + L+ P I    + K V ++   L  F    +E+D   Q+  Y+F
Sbjct: 276 GYFCRFGVSKEDAALLILRNPAIMNFDLEKPVISVTGMLKHFGLRQDEVDAVAQKYPYVF 335

Query: 369 -----RSHPLLLGSCTL-KKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPD 428
                ++ P +L +  L ++   +L N N     L  + L +P+E    +    +  L +
Sbjct: 336 GRNQLKNLPYVLRAIDLHERIFDILKNGN--HHLLASYTLMDPDEDLEREYQEGLEELQN 395

Query: 429 TGEVIRSKQQKTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYK 488
           +    R   QK  FL ++G  EN   M K L+   G   EL +RF  ++ +GI    +  
Sbjct: 396 S-RTKRHNIQKLDFLHEIGFGENGITM-KVLQHVHGTAVELHDRFQILLNSGIIFSKICM 455

Query: 489 MILVSPQILNQTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQ-RVTLRFSMYSWLK 548
           +I  +P+ILNQ    I++K+ FL   +G  +  L  FP+YL +  + R++ RF  + WL 
Sbjct: 456 LIRSAPKILNQKPHSIQDKLRFLCGEMGDSLDYLEVFPAYLCFDLENRISPRFRFHKWLV 515

Query: 549 EQGTADPMLALSTIVACSENTFLRQYVNHHPRGMEVW 567
           E+G ++   ++++IVA SE  F+ +    HP   + W
Sbjct: 516 EKGFSEKSYSIASIVATSEKAFIARLYGIHPAIPKHW 544

BLAST of Lsi04G008320 vs. ExPASy Swiss-Prot
Match: Q9C6A1 (Transcription termination factor MTERF15, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF15 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.0e-08
Identity = 32/127 (25.20%), Postives = 66/127 (51.97%), Query Frame = 0

Query: 448 GAELQERFDCIVEAGIDEKAVYKMILVSPQILNQTKDIIEEKIDFLVNNLGYPVSSLVNF 507
           G E++ R DC+ + G+  +  +K++   P+++    + IE+KI+FL N +G+ ++ L + 
Sbjct: 282 GFEVKLRVDCLCKYGLIRRDAFKVVWKEPRVILYEIEDIEKKIEFLTNRMGFHINCLADV 341

Query: 508 PSYLSYTTQR-VTLRFSMYSWLKEQGTADPMLALSTIVACSENTFLRQYVNHHPRGMEVW 567
           P YL    Q+ +  R+++  +LK +G     + L  ++  S   F   YV  +P    ++
Sbjct: 342 PEYLGVNLQKQIVPRYNVIDYLKLKGGLGCDIGLKGLIKPSMKRFYNLYVMPYPECERIF 401

Query: 568 QNLKREI 574
              K  +
Sbjct: 402 GKRKENV 408

BLAST of Lsi04G008320 vs. ExPASy TrEMBL
Match: A0A6J1DXM2 (transcription termination factor MTEF18, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111025051 PE=3 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.6e-287
Identity = 495/580 (85.34%), Postives = 534/580 (92.07%), Query Frame = 0

Query: 4   SSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGHRIS 63
           SSIIADNKFNLVR+L  +IGSS  A N RFYRTK+A Q  ECEN G + TR  RNGHRIS
Sbjct: 14  SSIIADNKFNLVRILSSQIGSSPAARNPRFYRTKRAHQAGECENFGGVPTRSGRNGHRIS 73

Query: 64  RAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRFLRY 123
           RA RKEAQAALLEYLHSTRG+Q +DAENMS+NSP+FLE+LLG+VE++GDI RSITRFLRY
Sbjct: 74  RATRKEAQAALLEYLHSTRGVQFMDAENMSKNSPLFLEKLLGKVENDGDIGRSITRFLRY 133

Query: 124 HPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKIYKE 183
           HPINEFEPFFES GL PAEYNAFLPR LMFL+DD LLLENY VL NYG+++NKIGKIYKE
Sbjct: 134 HPINEFEPFFESAGLHPAEYNAFLPRELMFLNDDGLLLENYRVLCNYGVEQNKIGKIYKE 193

Query: 184 ATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKNIGF 243
           ATQIF+YDYGVLLSKL+AYEEL LSQATVVKFVVCSPYLLVGGVN+GF +VLDKLKNIGF
Sbjct: 194 ATQIFRYDYGVLLSKLRAYEELDLSQATVVKFVVCSPYLLVGGVNEGFVKVLDKLKNIGF 253

Query: 244 ESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSLSLI 303
           ESSWVEEQLIDGNSYNWKQILGLLFWF++MGC NEKL DLISQHPDFLFEDSGSKSLSLI
Sbjct: 254 ESSWVEEQLIDGNSYNWKQILGLLFWFQKMGCSNEKLGDLISQHPDFLFEDSGSKSLSLI 313

Query: 304 GLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHPLLL 363
           G LLKMGSSMIQICS+F+QFPQIRVGKFVSN+R CL FFNEIDMGV+EIGYL RS PLLL
Sbjct: 314 GFLLKMGSSMIQICSMFMQFPQIRVGKFVSNIRRCLSFFNEIDMGVEEIGYLVRSQPLLL 373

Query: 364 GSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQKTQ 423
           GS TLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRV PLP  GE+ RSKQQKTQ
Sbjct: 374 GSYTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVTPLPVAGELKRSKQQKTQ 433

Query: 424 FLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILNQTK 483
           FLLKLGLEENSS+MK+ALKVFRGKGAELQERFDCI++AGIDEK VYKMI VSPQILNQTK
Sbjct: 434 FLLKLGLEENSSKMKEALKVFRGKGAELQERFDCIIKAGIDEKDVYKMIEVSPQILNQTK 493

Query: 484 DIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLALSTI 543
           D IEEKIDFL+N+LGYPVSSLVNFPSYLSYTTQRVTLRFSMY+WLKEQGTADP LALSTI
Sbjct: 494 DKIEEKIDFLLNDLGYPVSSLVNFPSYLSYTTQRVTLRFSMYTWLKEQGTADPTLALSTI 553

Query: 544 VACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPAH 584
           +ACSENTFLRQYVNHHP+G+E WQ LKREIYSDS++   H
Sbjct: 554 IACSENTFLRQYVNHHPQGVEFWQKLKREIYSDSLSCLTH 593

BLAST of Lsi04G008320 vs. ExPASy TrEMBL
Match: A0A6J1HNT9 (transcription termination factor MTEF18, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111465965 PE=3 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.8e-284
Identity = 498/582 (85.57%), Postives = 530/582 (91.07%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           +V+SSIIADNKFNLVRVLYWRIG S V SN RFYRTKKAPQ+ EC NS    T  S NGH
Sbjct: 12  LVASSIIADNKFNLVRVLYWRIGFSPVFSNPRFYRTKKAPQSGECGNS----TTGSGNGH 71

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
           RISRAIRKEAQ ALLEYLHSTRGIQ +DAENMS+NSPIFL +LLGRVE+EG I RSITRF
Sbjct: 72  RISRAIRKEAQDALLEYLHSTRGIQFMDAENMSKNSPIFLAKLLGRVENEGKIGRSITRF 131

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFESVGLQPAEYN FLPR +MFLSDDDLLLEN HVL NYGI+R+KIGKI
Sbjct: 132 LRYHPINEFEPFFESVGLQPAEYNEFLPREVMFLSDDDLLLENCHVLCNYGIERSKIGKI 191

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           YKEATQIF+YDYGVLLSKLKAYEELGL QATVVKFVVC+PYLLVGGVN+GF +VLDKLKN
Sbjct: 192 YKEATQIFRYDYGVLLSKLKAYEELGLDQATVVKFVVCNPYLLVGGVNNGFVKVLDKLKN 251

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFE SWVEEQL+DGNSY WKQILGLLFWFK MGC +EKLADLI  HPDFLFEDSGSKSL
Sbjct: 252 IGFEPSWVEEQLLDGNSYYWKQILGLLFWFKHMGCSDEKLADLIRLHPDFLFEDSGSKSL 311

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIGLLLKMGS M+QI SVFLQFPQ+RVGKFVSN+R CLLFFNEIDM VQ+IGYLFRSHP
Sbjct: 312 SLIGLLLKMGSPMMQIYSVFLQFPQMRVGKFVSNIRRCLLFFNEIDMCVQQIGYLFRSHP 371

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQ 420
           LLLGSCTLKKTNSLL+ LNVGKKRLCQFILENPEELKNWKL TRV PLPD GE +RSKQQ
Sbjct: 372 LLLGSCTLKKTNSLLSILNVGKKRLCQFILENPEELKNWKLRTRVLPLPDNGEDVRSKQQ 431

Query: 421 KTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILN 480
           KTQFLLKLGL+ENSS+MKKA KVFRGKG ELQERFDCIVEAGI+EK VY+MI VSPQILN
Sbjct: 432 KTQFLLKLGLKENSSKMKKAFKVFRGKGEELQERFDCIVEAGIEEKDVYRMIKVSPQILN 491

Query: 481 QTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLAL 540
           Q+KDIIE KIDFLVN  GYPVSSLVNF SYLSY+ QRVTLR SMYSWLKEQG ADP LAL
Sbjct: 492 QSKDIIEIKIDFLVNTFGYPVSSLVNFSSYLSYSAQRVTLRLSMYSWLKEQGAADPTLAL 551

Query: 541 STIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPA 583
            TIVACSENTFL+QYVNHHP+GMEVW+NLKREIYSDS+ASPA
Sbjct: 552 RTIVACSENTFLKQYVNHHPKGMEVWENLKREIYSDSLASPA 589

BLAST of Lsi04G008320 vs. ExPASy TrEMBL
Match: A0A6J1EEL8 (transcription termination factor MTEF18, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432534 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.3e-278
Identity = 488/582 (83.85%), Postives = 524/582 (90.03%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           + +SSIIADNKFNLVRVLYWRIG S V  N RFY TKK+PQ+ EC NS    T  S NGH
Sbjct: 12  LAASSIIADNKFNLVRVLYWRIGFSPVFLNPRFYGTKKSPQSGECGNS----TTGSGNGH 71

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
           RISRAIRKEAQ ALLEYLHSTRGIQ +DAENMS+NSPIFL +LLGRVE+EG+I RSITRF
Sbjct: 72  RISRAIRKEAQDALLEYLHSTRGIQFMDAENMSKNSPIFLAKLLGRVENEGEIGRSITRF 131

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFESVGLQPAEYN FLPR +MFLSDDDLLLENYHVL NYGI+RNKIGKI
Sbjct: 132 LRYHPINEFEPFFESVGLQPAEYNEFLPREVMFLSDDDLLLENYHVLCNYGIERNKIGKI 191

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           YKEATQIF YDYGVLLSKLKAYEELGL Q TVV+FVVCSPYLLVGGV + F +VLDKLKN
Sbjct: 192 YKEATQIFWYDYGVLLSKLKAYEELGLDQPTVVQFVVCSPYLLVGGVKNWFVKVLDKLKN 251

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFE SWVEEQL+DG+SYNWKQILGLLFWFK MGC +EKLADLI   PDFLFEDSGSKSL
Sbjct: 252 IGFEPSWVEEQLLDGHSYNWKQILGLLFWFKHMGCSDEKLADLIRLRPDFLFEDSGSKSL 311

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIGLLLKMGSSM+QI SVFLQFPQ+RVGKFVSN+R CLLFFNEIDMGVQ+IGYLFRSHP
Sbjct: 312 SLIGLLLKMGSSMMQIYSVFLQFPQMRVGKFVSNIRQCLLFFNEIDMGVQQIGYLFRSHP 371

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQ 420
           LLLGSCTLKKTNSLL+ LNVGKKRL Q ILENPEELKNW+LGTRV PLPD GEV RSKQQ
Sbjct: 372 LLLGSCTLKKTNSLLSILNVGKKRLRQIILENPEELKNWRLGTRVLPLPDNGEVERSKQQ 431

Query: 421 KTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILN 480
           KTQFLLKLGLEENSS+MK+A KVFRGKG ELQERFDCIVEAGIDEK V ++I+VSPQILN
Sbjct: 432 KTQFLLKLGLEENSSKMKEAFKVFRGKGEELQERFDCIVEAGIDEKDVRRLIIVSPQILN 491

Query: 481 QTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLAL 540
           Q+KDIIE KID LVN  GYPVSSLVNF SYLSY+ QRVTLR SMYSWLKEQG ADP LAL
Sbjct: 492 QSKDIIELKIDLLVNTFGYPVSSLVNFSSYLSYSAQRVTLRLSMYSWLKEQGAADPTLAL 551

Query: 541 STIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPA 583
            T+VACSEN FL+QYV+HHP+GMEVW+NLKREIYSDS+ASPA
Sbjct: 552 RTVVACSENRFLKQYVHHHPKGMEVWENLKREIYSDSLASPA 589

BLAST of Lsi04G008320 vs. ExPASy TrEMBL
Match: A0A5D3DAD9 (Transcription termination factor MTEF18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G001060 PE=3 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 4.8e-276
Identity = 483/585 (82.56%), Postives = 520/585 (88.89%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           M+SSSIIA NKFN V+V Y RIG S VASN RFYRTK+ P+TEEC NSG +L   SR GH
Sbjct: 1   MLSSSIIAHNKFNFVQVSYRRIGFSPVASNPRFYRTKETPKTEECGNSGGMLNMGSRKGH 60

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
            I+RA  KEAQA LLEYLH TRGIQ +DAENMS+NSPIFLE+LLG+VEHEG+I  SI +F
Sbjct: 61  EITRATIKEAQATLLEYLHFTRGIQFLDAENMSKNSPIFLEKLLGKVEHEGNIGWSIMQF 120

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSD DLLLEN+HVL NYGI+RNK GKI
Sbjct: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDADLLLENFHVLCNYGIERNKTGKI 180

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           ++EAT+IF+YDYG+LLSKLKAYE  GLSQATVVKFVVCSP LL+ GVND F QVL+KLKN
Sbjct: 181 FREATEIFRYDYGILLSKLKAYENFGLSQATVVKFVVCSPRLLIDGVNDVFVQVLEKLKN 240

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFESSWVE+QL DGNSYNWKQILGLLF F+QMGC  EKLADLISQHPD LFEDSGSKSL
Sbjct: 241 IGFESSWVEKQLRDGNSYNWKQILGLLFLFEQMGCSKEKLADLISQHPDLLFEDSGSKSL 300

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIG LLKMG SMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP
Sbjct: 301 SLIGFLLKMGCSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIR--SK 420
           LLLG CTLKKT+SLL NLN GKKR+CQFILENPEELKNWKLGTRV  LPD G   +  + 
Sbjct: 361 LLLGLCTLKKTSSLLINLNAGKKRICQFILENPEELKNWKLGTRVLQLPDPGGKGKKYAN 420

Query: 421 QQKTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQI 480
           QQK QFLLKLGLEENS++MKKALK F GKG ELQERF+CIVEAGIDEK VYKMI V P++
Sbjct: 421 QQKIQFLLKLGLEENSTKMKKALKTFEGKGVELQERFNCIVEAGIDEKDVYKMIEVCPKM 480

Query: 481 LNQTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPML 540
           LNQ KDIIEEKIDFLVNNLG PVS+LV FPSYLSYTTQRVTLR SMYSWLK+QGTADPML
Sbjct: 481 LNQKKDIIEEKIDFLVNNLGLPVSTLVTFPSYLSYTTQRVTLRISMYSWLKKQGTADPML 540

Query: 541 ALSTIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPAH 584
            LST+V CSENTFLRQYVNHHPRGMEVW+NLKREIYSDS+ SPAH
Sbjct: 541 KLSTMVVCSENTFLRQYVNHHPRGMEVWENLKREIYSDSMVSPAH 585

BLAST of Lsi04G008320 vs. ExPASy TrEMBL
Match: A0A1S4DWM2 (transcription termination factor MTEF18, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489881 PE=3 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 4.8e-276
Identity = 483/585 (82.56%), Postives = 520/585 (88.89%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           M+SSSIIA NKFN V+V Y RIG S VASN RFYRTK+ P+TEEC NSG +L   SR GH
Sbjct: 12  MLSSSIIAHNKFNFVQVSYRRIGFSPVASNPRFYRTKETPKTEECGNSGGMLNMGSRKGH 71

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
            I+RA  KEAQA LLEYLH TRGIQ +DAENMS+NSPIFLE+LLG+VEHEG+I  SI +F
Sbjct: 72  EITRATIKEAQATLLEYLHFTRGIQFLDAENMSKNSPIFLEKLLGKVEHEGNIGWSIMQF 131

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSD DLLLEN+HVL NYGI+RNK GKI
Sbjct: 132 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDADLLLENFHVLCNYGIERNKTGKI 191

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           ++EAT+IF+YDYG+LLSKLKAYE  GLSQATVVKFVVCSP LL+ GVND F QVL+KLKN
Sbjct: 192 FREATEIFRYDYGILLSKLKAYENFGLSQATVVKFVVCSPRLLIDGVNDVFVQVLEKLKN 251

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFESSWVE+QL DGNSYNWKQILGLLF F+QMGC  EKLADLISQHPD LFEDSGSKSL
Sbjct: 252 IGFESSWVEKQLRDGNSYNWKQILGLLFLFEQMGCSKEKLADLISQHPDLLFEDSGSKSL 311

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIG LLKMG SMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP
Sbjct: 312 SLIGFLLKMGCSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 371

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIR--SK 420
           LLLG CTLKKT+SLL NLN GKKR+CQFILENPEELKNWKLGTRV  LPD G   +  + 
Sbjct: 372 LLLGLCTLKKTSSLLINLNAGKKRICQFILENPEELKNWKLGTRVLQLPDPGGKGKKYAN 431

Query: 421 QQKTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQI 480
           QQK QFLLKLGLEENS++MKKALK F GKG ELQERF+CIVEAGIDEK VYKMI V P++
Sbjct: 432 QQKIQFLLKLGLEENSTKMKKALKTFEGKGVELQERFNCIVEAGIDEKDVYKMIEVCPKM 491

Query: 481 LNQTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPML 540
           LNQ KDIIEEKIDFLVNNLG PVS+LV FPSYLSYTTQRVTLR SMYSWLK+QGTADPML
Sbjct: 492 LNQKKDIIEEKIDFLVNNLGLPVSTLVTFPSYLSYTTQRVTLRISMYSWLKKQGTADPML 551

Query: 541 ALSTIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPAH 584
            LST+V CSENTFLRQYVNHHPRGMEVW+NLKREIYSDS+ SPAH
Sbjct: 552 KLSTMVVCSENTFLRQYVNHHPRGMEVWENLKREIYSDSMVSPAH 596

BLAST of Lsi04G008320 vs. NCBI nr
Match: XP_038891122.1 (transcription termination factor MTEF18, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 1007.7 bits (2604), Expect = 4.2e-290
Identity = 510/578 (88.24%), Postives = 535/578 (92.56%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           MVSSSIIADNKF LVRVLYWRIG S VASN RFYRTKKAPQ+EECENSG   T+V+RNG 
Sbjct: 12  MVSSSIIADNKFKLVRVLYWRIGFSPVASNPRFYRTKKAPQSEECENSGGNSTKVNRNGR 71

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
           RISRA  KEAQAALLEYL+STRGIQ + AENMSRNSP FLE+LL RVEHEGDI RS+ RF
Sbjct: 72  RISRATMKEAQAALLEYLNSTRGIQLMVAENMSRNSPTFLEKLLERVEHEGDIGRSVLRF 131

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFES+GLQPAEYNAFLPRNLMFL+DDDLLL+NY VL +YGI++NKIGKI
Sbjct: 132 LRYHPINEFEPFFESLGLQPAEYNAFLPRNLMFLNDDDLLLQNYLVLCSYGIEQNKIGKI 191

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           YKEATQIFQ DYGVLLSKLKAYE+LGLS+ATV KFVVCSPYLL+GGVNDGFAQVLDKLKN
Sbjct: 192 YKEATQIFQCDYGVLLSKLKAYEKLGLSRATVAKFVVCSPYLLIGGVNDGFAQVLDKLKN 251

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFESSWVEEQL D +SYNWK+ILGLLFWFKQMG CNEKLADLI QHPDFLFEDSGSKSL
Sbjct: 252 IGFESSWVEEQLTDDSSYNWKKILGLLFWFKQMGYCNEKLADLIRQHPDFLFEDSGSKSL 311

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIGLLLKMGSSM QICSVFLQFPQIRV KFVSNMR CLLFF+EIDMGVQEIGYLFRSHP
Sbjct: 312 SLIGLLLKMGSSMNQICSVFLQFPQIRVSKFVSNMRQCLLFFSEIDMGVQEIGYLFRSHP 371

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQ 420
           LLLG CTLKKT+SLLANLNVGKKRLCQFILE PEELKNWKLGTRVPPLPD+    RSKQQ
Sbjct: 372 LLLGLCTLKKTSSLLANLNVGKKRLCQFILEKPEELKNWKLGTRVPPLPDSEN--RSKQQ 431

Query: 421 KTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILN 480
           KTQFLLKLGLEENS EMK+A KVFRGKGAELQERFDCIVEAGIDEK VYKMI VSPQILN
Sbjct: 432 KTQFLLKLGLEENSREMKEAFKVFRGKGAELQERFDCIVEAGIDEKDVYKMIEVSPQILN 491

Query: 481 QTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLAL 540
           QTK IIEEKIDFL+NNLGYP SSLV+FPSYLSYTTQRVTLRF MYSWLKEQGTADPMLAL
Sbjct: 492 QTKYIIEEKIDFLLNNLGYPASSLVSFPSYLSYTTQRVTLRFLMYSWLKEQGTADPMLAL 551

Query: 541 STIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSI 579
           STIVACSE  FLRQYVNHHPRGMEVWQ LKREIYSDS+
Sbjct: 552 STIVACSEKKFLRQYVNHHPRGMEVWQKLKREIYSDSM 587

BLAST of Lsi04G008320 vs. NCBI nr
Match: XP_022158597.1 (transcription termination factor MTEF18, mitochondrial-like [Momordica charantia])

HSP 1 Score: 998.0 bits (2579), Expect = 3.3e-287
Identity = 495/580 (85.34%), Postives = 534/580 (92.07%), Query Frame = 0

Query: 4   SSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGHRIS 63
           SSIIADNKFNLVR+L  +IGSS  A N RFYRTK+A Q  ECEN G + TR  RNGHRIS
Sbjct: 14  SSIIADNKFNLVRILSSQIGSSPAARNPRFYRTKRAHQAGECENFGGVPTRSGRNGHRIS 73

Query: 64  RAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRFLRY 123
           RA RKEAQAALLEYLHSTRG+Q +DAENMS+NSP+FLE+LLG+VE++GDI RSITRFLRY
Sbjct: 74  RATRKEAQAALLEYLHSTRGVQFMDAENMSKNSPLFLEKLLGKVENDGDIGRSITRFLRY 133

Query: 124 HPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKIYKE 183
           HPINEFEPFFES GL PAEYNAFLPR LMFL+DD LLLENY VL NYG+++NKIGKIYKE
Sbjct: 134 HPINEFEPFFESAGLHPAEYNAFLPRELMFLNDDGLLLENYRVLCNYGVEQNKIGKIYKE 193

Query: 184 ATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKNIGF 243
           ATQIF+YDYGVLLSKL+AYEEL LSQATVVKFVVCSPYLLVGGVN+GF +VLDKLKNIGF
Sbjct: 194 ATQIFRYDYGVLLSKLRAYEELDLSQATVVKFVVCSPYLLVGGVNEGFVKVLDKLKNIGF 253

Query: 244 ESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSLSLI 303
           ESSWVEEQLIDGNSYNWKQILGLLFWF++MGC NEKL DLISQHPDFLFEDSGSKSLSLI
Sbjct: 254 ESSWVEEQLIDGNSYNWKQILGLLFWFQKMGCSNEKLGDLISQHPDFLFEDSGSKSLSLI 313

Query: 304 GLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHPLLL 363
           G LLKMGSSMIQICS+F+QFPQIRVGKFVSN+R CL FFNEIDMGV+EIGYL RS PLLL
Sbjct: 314 GFLLKMGSSMIQICSMFMQFPQIRVGKFVSNIRRCLSFFNEIDMGVEEIGYLVRSQPLLL 373

Query: 364 GSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQKTQ 423
           GS TLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRV PLP  GE+ RSKQQKTQ
Sbjct: 374 GSYTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVTPLPVAGELKRSKQQKTQ 433

Query: 424 FLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILNQTK 483
           FLLKLGLEENSS+MK+ALKVFRGKGAELQERFDCI++AGIDEK VYKMI VSPQILNQTK
Sbjct: 434 FLLKLGLEENSSKMKEALKVFRGKGAELQERFDCIIKAGIDEKDVYKMIEVSPQILNQTK 493

Query: 484 DIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLALSTI 543
           D IEEKIDFL+N+LGYPVSSLVNFPSYLSYTTQRVTLRFSMY+WLKEQGTADP LALSTI
Sbjct: 494 DKIEEKIDFLLNDLGYPVSSLVNFPSYLSYTTQRVTLRFSMYTWLKEQGTADPTLALSTI 553

Query: 544 VACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPAH 584
           +ACSENTFLRQYVNHHP+G+E WQ LKREIYSDS++   H
Sbjct: 554 IACSENTFLRQYVNHHPQGVEFWQKLKREIYSDSLSCLTH 593

BLAST of Lsi04G008320 vs. NCBI nr
Match: XP_038882474.1 (transcription termination factor MTEF18, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 997.3 bits (2577), Expect = 5.6e-287
Identity = 495/583 (84.91%), Postives = 537/583 (92.11%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           M+SSSIIADNKFN VRVLYW+IG S VAS  RFYRTKKAPQT+EC NSGE+L   SRNGH
Sbjct: 12  MLSSSIIADNKFNFVRVLYWQIGFSPVASGPRFYRTKKAPQTDECGNSGEMLNMGSRNGH 71

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
           RISRA RKEAQAALLEYLHSTRGIQ +DAENMS+NSPIFL +LLGRVEHEGDI RSITRF
Sbjct: 72  RISRATRKEAQAALLEYLHSTRGIQLMDAENMSKNSPIFLGKLLGRVEHEGDIGRSITRF 131

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFESVGLQPAEY+ FLPRNLMFLSDDDLLLENYHVL NYGI+RNKIGKI
Sbjct: 132 LRYHPINEFEPFFESVGLQPAEYDGFLPRNLMFLSDDDLLLENYHVLCNYGIERNKIGKI 191

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           YKEATQIFQYDYGVLLSKLKAYE+LG+ QATVVKFVVCSPYLLVGGV+DGF QVLDKLKN
Sbjct: 192 YKEATQIFQYDYGVLLSKLKAYEKLGIGQATVVKFVVCSPYLLVGGVDDGFVQVLDKLKN 251

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFESSWVEEQL D NSYNWK+ILGLLF F+QMGC +EKLADLISQHPDFLFEDSGSKSL
Sbjct: 252 IGFESSWVEEQLTDCNSYNWKKILGLLFRFEQMGCSDEKLADLISQHPDFLFEDSGSKSL 311

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIGLLLKMG SMIQICSV LQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIG LFRSHP
Sbjct: 312 SLIGLLLKMGCSMIQICSVLLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGCLFRSHP 371

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQ 420
           LLLG CTLKK +SLL  LNVGKKRLCQFILENPEELKNWKLGT+V PLP++GE++RSKQQ
Sbjct: 372 LLLGLCTLKKASSLLHLLNVGKKRLCQFILENPEELKNWKLGTKVSPLPNSGEIVRSKQQ 431

Query: 421 KTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILN 480
           KTQFLLKLGLEENS++M++AL+ F+GKG ELQERFDCIVEAGIDEK VYKMI VSP+ILN
Sbjct: 432 KTQFLLKLGLEENSTKMEEALQAFQGKGTELQERFDCIVEAGIDEKDVYKMIEVSPRILN 491

Query: 481 QTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLAL 540
           QTKD++EEKIDF +NNLGYPVSSL++FPSYL Y TQRVTLR +M +WLKEQG   PMLAL
Sbjct: 492 QTKDMLEEKIDFFLNNLGYPVSSLISFPSYLCYATQRVTLRIAMCNWLKEQGIVLPMLAL 551

Query: 541 STIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPAH 584
            TI+ CS++TFLR+YVNHHPRG EVW+NLKREIYSDS+ S A+
Sbjct: 552 RTILVCSDDTFLRRYVNHHPRGTEVWENLKREIYSDSMTSLAY 594

BLAST of Lsi04G008320 vs. NCBI nr
Match: XP_022966246.1 (transcription termination factor MTEF18, mitochondrial-like [Cucurbita maxima] >XP_022966247.1 transcription termination factor MTEF18, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 987.3 bits (2551), Expect = 5.8e-284
Identity = 498/582 (85.57%), Postives = 530/582 (91.07%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           +V+SSIIADNKFNLVRVLYWRIG S V SN RFYRTKKAPQ+ EC NS    T  S NGH
Sbjct: 12  LVASSIIADNKFNLVRVLYWRIGFSPVFSNPRFYRTKKAPQSGECGNS----TTGSGNGH 71

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
           RISRAIRKEAQ ALLEYLHSTRGIQ +DAENMS+NSPIFL +LLGRVE+EG I RSITRF
Sbjct: 72  RISRAIRKEAQDALLEYLHSTRGIQFMDAENMSKNSPIFLAKLLGRVENEGKIGRSITRF 131

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFESVGLQPAEYN FLPR +MFLSDDDLLLEN HVL NYGI+R+KIGKI
Sbjct: 132 LRYHPINEFEPFFESVGLQPAEYNEFLPREVMFLSDDDLLLENCHVLCNYGIERSKIGKI 191

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           YKEATQIF+YDYGVLLSKLKAYEELGL QATVVKFVVC+PYLLVGGVN+GF +VLDKLKN
Sbjct: 192 YKEATQIFRYDYGVLLSKLKAYEELGLDQATVVKFVVCNPYLLVGGVNNGFVKVLDKLKN 251

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFE SWVEEQL+DGNSY WKQILGLLFWFK MGC +EKLADLI  HPDFLFEDSGSKSL
Sbjct: 252 IGFEPSWVEEQLLDGNSYYWKQILGLLFWFKHMGCSDEKLADLIRLHPDFLFEDSGSKSL 311

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIGLLLKMGS M+QI SVFLQFPQ+RVGKFVSN+R CLLFFNEIDM VQ+IGYLFRSHP
Sbjct: 312 SLIGLLLKMGSPMMQIYSVFLQFPQMRVGKFVSNIRRCLLFFNEIDMCVQQIGYLFRSHP 371

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQ 420
           LLLGSCTLKKTNSLL+ LNVGKKRLCQFILENPEELKNWKL TRV PLPD GE +RSKQQ
Sbjct: 372 LLLGSCTLKKTNSLLSILNVGKKRLCQFILENPEELKNWKLRTRVLPLPDNGEDVRSKQQ 431

Query: 421 KTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILN 480
           KTQFLLKLGL+ENSS+MKKA KVFRGKG ELQERFDCIVEAGI+EK VY+MI VSPQILN
Sbjct: 432 KTQFLLKLGLKENSSKMKKAFKVFRGKGEELQERFDCIVEAGIEEKDVYRMIKVSPQILN 491

Query: 481 QTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLAL 540
           Q+KDIIE KIDFLVN  GYPVSSLVNF SYLSY+ QRVTLR SMYSWLKEQG ADP LAL
Sbjct: 492 QSKDIIEIKIDFLVNTFGYPVSSLVNFSSYLSYSAQRVTLRLSMYSWLKEQGAADPTLAL 551

Query: 541 STIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPA 583
            TIVACSENTFL+QYVNHHP+GMEVW+NLKREIYSDS+ASPA
Sbjct: 552 RTIVACSENTFLKQYVNHHPKGMEVWENLKREIYSDSLASPA 589

BLAST of Lsi04G008320 vs. NCBI nr
Match: XP_022925228.1 (transcription termination factor MTEF18, mitochondrial-like isoform X1 [Cucurbita moschata] >XP_022925229.1 transcription termination factor MTEF18, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 967.6 bits (2500), Expect = 4.8e-278
Identity = 488/582 (83.85%), Postives = 524/582 (90.03%), Query Frame = 0

Query: 1   MVSSSIIADNKFNLVRVLYWRIGSSSVASNARFYRTKKAPQTEECENSGEILTRVSRNGH 60
           + +SSIIADNKFNLVRVLYWRIG S V  N RFY TKK+PQ+ EC NS    T  S NGH
Sbjct: 12  LAASSIIADNKFNLVRVLYWRIGFSPVFLNPRFYGTKKSPQSGECGNS----TTGSGNGH 71

Query: 61  RISRAIRKEAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHEGDIARSITRF 120
           RISRAIRKEAQ ALLEYLHSTRGIQ +DAENMS+NSPIFL +LLGRVE+EG+I RSITRF
Sbjct: 72  RISRAIRKEAQDALLEYLHSTRGIQFMDAENMSKNSPIFLAKLLGRVENEGEIGRSITRF 131

Query: 121 LRYHPINEFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKI 180
           LRYHPINEFEPFFESVGLQPAEYN FLPR +MFLSDDDLLLENYHVL NYGI+RNKIGKI
Sbjct: 132 LRYHPINEFEPFFESVGLQPAEYNEFLPREVMFLSDDDLLLENYHVLCNYGIERNKIGKI 191

Query: 181 YKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKN 240
           YKEATQIF YDYGVLLSKLKAYEELGL Q TVV+FVVCSPYLLVGGV + F +VLDKLKN
Sbjct: 192 YKEATQIFWYDYGVLLSKLKAYEELGLDQPTVVQFVVCSPYLLVGGVKNWFVKVLDKLKN 251

Query: 241 IGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSL 300
           IGFE SWVEEQL+DG+SYNWKQILGLLFWFK MGC +EKLADLI   PDFLFEDSGSKSL
Sbjct: 252 IGFEPSWVEEQLLDGHSYNWKQILGLLFWFKHMGCSDEKLADLIRLRPDFLFEDSGSKSL 311

Query: 301 SLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHP 360
           SLIGLLLKMGSSM+QI SVFLQFPQ+RVGKFVSN+R CLLFFNEIDMGVQ+IGYLFRSHP
Sbjct: 312 SLIGLLLKMGSSMMQIYSVFLQFPQMRVGKFVSNIRQCLLFFNEIDMGVQQIGYLFRSHP 371

Query: 361 LLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVIRSKQQ 420
           LLLGSCTLKKTNSLL+ LNVGKKRL Q ILENPEELKNW+LGTRV PLPD GEV RSKQQ
Sbjct: 372 LLLGSCTLKKTNSLLSILNVGKKRLRQIILENPEELKNWRLGTRVLPLPDNGEVERSKQQ 431

Query: 421 KTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILN 480
           KTQFLLKLGLEENSS+MK+A KVFRGKG ELQERFDCIVEAGIDEK V ++I+VSPQILN
Sbjct: 432 KTQFLLKLGLEENSSKMKEAFKVFRGKGEELQERFDCIVEAGIDEKDVRRLIIVSPQILN 491

Query: 481 QTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLAL 540
           Q+KDIIE KID LVN  GYPVSSLVNF SYLSY+ QRVTLR SMYSWLKEQG ADP LAL
Sbjct: 492 QSKDIIELKIDLLVNTFGYPVSSLVNFSSYLSYSAQRVTLRLSMYSWLKEQGAADPTLAL 551

Query: 541 STIVACSENTFLRQYVNHHPRGMEVWQNLKREIYSDSIASPA 583
            T+VACSEN FL+QYV+HHP+GMEVW+NLKREIYSDS+ASPA
Sbjct: 552 RTVVACSENRFLKQYVHHHPKGMEVWENLKREIYSDSLASPA 589

BLAST of Lsi04G008320 vs. TAIR 10
Match: AT5G06810.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 514.6 bits (1324), Expect = 1.0e-145
Identity = 267/555 (48.11%), Postives = 377/555 (67.93%), Query Frame = 0

Query: 27   VASNARFYRTKKAPQTEECENSGEILTRVSRNGHRISRAIRKEAQAALLEYLHSTRGIQH 86
            ++ N RF+ T++A    + E SGE      + G R    IRK AQ A+ +Y + TRG+Q 
Sbjct: 591  LSRNPRFFATQRA--LVDAEVSGE------KWGLRTRNEIRKVAQVAMFDYFYQTRGLQF 650

Query: 87   VDAENMSRNSPIFLEELLGR-------VEHEGDIARSITRFLRYHPINEFEPFFESVGLQ 146
            + AE+MS+N+P+F + LL +       V+ + DI ++ITRFL +HP+NEFEPF ES+GL+
Sbjct: 651  LVAESMSKNAPVFNDNLLKKLNGCDVDVDDDDDIVKAITRFLWFHPVNEFEPFLESLGLK 710

Query: 147  PAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKIYKEATQIFQYDYGVLLSKL 206
            P+E++  +P + MFL++D  LLENYHV  NYGI R K+GKI+KEA ++F Y+ GVL SK+
Sbjct: 711  PSEFSHLIPCDKMFLNEDAFLLENYHVFWNYGIGREKMGKIFKEAREVFGYETGVLASKI 770

Query: 207  KAYEELGLSQATVVKFVVCSPYLLVGGVNDGFAQVLDKLKNIGFESSWVEEQLIDGNSYN 266
            K+YE+LG S+  + K +VCSP +L+G +N G A+V++ LK IGF   WV E L +  SY+
Sbjct: 771  KSYEDLGFSKLFLSKLIVCSPSILIGDMNVGLAKVMEMLKAIGFGVDWVTENLSEEVSYD 830

Query: 267  WKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSLSLIGLLLKMGSSMIQICSV 326
            W  +   L + + +     +L +LI + P  +FEDSG  +L L G   K+GSS  ++ S+
Sbjct: 831  WSSMHRCLSFLRDLYVDENELCELIRKMPRLIFEDSGEWTLILAGFEAKLGSSRSELSSL 890

Query: 327  FLQFPQIR-VGKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHPLLLGSCTLKKTNSLLANL 386
            F +FPQ + +GKFV N+R C LF  +I+M   EIG +FR H L +G   LK+T++LL NL
Sbjct: 891  FQKFPQCQSLGKFVLNLRHCFLFLKDIEMDDDEIGKIFRLHSLWIGVSRLKQTSTLLINL 950

Query: 387  NVGKKRLCQFILENPEELKNWKLGTRVPPLPDTGEVI--RSKQQKTQFLLKLGLEENSSE 446
              GK RLCQ I ENPEE+K W +G RV PLP TG  +  +SK  KTQFLL LG +ENS E
Sbjct: 951  KGGKGRLCQVIQENPEEMKKWIMGLRVQPLPATGYKVNTKSKTMKTQFLLDLGYKENSEE 1010

Query: 447  MKKALKVFRGKGAELQERFDCIVEAGIDEKAVYKMILVSPQILNQTKDIIEEKIDFLVNN 506
            M++ALK FRGKG+EL+ERF+ +V  G+ EK V  M+   P IL Q  DI+E K+++LV  
Sbjct: 1011 MERALKNFRGKGSELRERFNVLVSFGLTEKDVKDMVKACPSILTQACDILESKVNYLVKE 1070

Query: 507  LGYPVSSLVNFPSYLSYTTQRVTLRFSMYSWLKEQGTADPMLALSTIVACSENTFLRQYV 566
            LGYP+S+LV FP+ L YT QR+ LRFSM+SWL+++G ADP L +STI+ CS+  F  ++V
Sbjct: 1071 LGYPLSTLVTFPTCLKYTLQRMKLRFSMFSWLQDRGKADPKLQVSTILVCSDKFFATRFV 1130

Query: 567  NHHPRGMEVWQNLKR 572
            N HP G +  ++LK+
Sbjct: 1131 NRHPDGPKHLEDLKK 1137

BLAST of Lsi04G008320 vs. TAIR 10
Match: AT4G19650.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 432.6 bits (1111), Expect = 5.1e-121
Identity = 218/483 (45.13%), Postives = 315/483 (65.22%), Query Frame = 0

Query: 90  ENMSRNSPIFLEELLGRV-EHEGDIARSITRFLRYHPINEFEPFFESVGLQPAEYNAFLP 149
           E++S+NSP F+  LL ++ +++ D+++ +T+FLRY+PINEFEPFFES+GL P E+  FLP
Sbjct: 90  EHISKNSPCFMSTLLSKIDDNQKDVSKGLTKFLRYNPINEFEPFFESLGLCPYEFETFLP 149

Query: 150 RNLMFLSDDDLLLENYHVLRNYGIQRNKIGKIYKEATQIFQYDYGVLLSKLKAYEELGLS 209
           R LMFLSDD ++ EN+H L NYGI R KIG++YKEA +IF+Y+ G+L  KL+ YE LGLS
Sbjct: 150 RKLMFLSDDGIMFENFHALCNYGIPRGKIGRMYKEAREIFRYESGMLAMKLRGYENLGLS 209

Query: 210 QATVVKFVVCSPYLLVGGVNDGFAQVLDKLKNIGFESSWVEEQLIDGNSYNWKQILGLLF 269
           +ATV+K V   P LLVGG++  F+ V+DKLK +     W+   L D  +Y+W++IL  + 
Sbjct: 210 KATVIKLVTSCPLLLVGGIDAEFSSVVDKLKGLQVGCDWLGRYLSDRKTYSWRRILETIE 269

Query: 270 WFKQMGCCNEKLADLISQHPDFLFEDSGSKSLSLIGLLLKMGSSMIQICSVFLQFPQIRV 329
           +  ++GC  EKL+ L+  +P  + E SG K   L G L K G  + +I  +F+  P++  
Sbjct: 270 FLDKVGCKEEKLSSLLKTYPALVIEGSGKKFYVLFGRLFKAGLQVNEIYRLFIDNPEMLS 329

Query: 330 GKFVSNMRLCLLFFNEIDMGVQEIGYLFRSHPLLLGSCTLKKTNSLLANLNVGKKRLCQF 389
            K V N++  L F   I M  Q I  +  SH  L+GSC+L    +   +LNV +  LC+ 
Sbjct: 330 DKCVKNIQKTLDFLIAIRMETQFITKILLSHMELIGSCSLPAPRTACLSLNVKQDELCKI 389

Query: 390 ILENPEELKNWKLGTRVPPLPDTGEVIRSKQQKTQFLLKLGLEENSSEMKKALKVFRGKG 449
           + + P  L  +   T+        E  R   +KT+FLL+LG  ENS EM KALK FRG+G
Sbjct: 390 LKKEPLRLFCFVSTTKKRKSKPLSEDSRKYLEKTEFLLRLGYVENSDEMVKALKQFRGRG 449

Query: 450 AELQERFDCIVEAGIDEKAVYKMILVSPQILNQTKDIIEEKIDFLVNNLGYPVSSLVNFP 509
            +LQERFDC+V+AG++   V ++I  +P ILN +KD+IE+KI  L   LGYP+ SLV FP
Sbjct: 450 DQLQERFDCLVKAGLNYNVVTEIIRHAPMILNLSKDVIEKKIHSLTELLGYPIESLVRFP 509

Query: 510 SYLSYTTQRVTLRFSMYSWLKEQGTADPMLALSTIVACSENTFLRQYVNHHPRGMEVWQN 569
           +YL Y  QR+  RFSMY WL+E+  A PML+ STI+ C +  F++ +VN HP G  +W++
Sbjct: 510 AYLCYDMQRIHHRFSMYLWLRERDAAKPMLSPSTILTCGDARFVKYFVNVHPEGPAIWES 569

Query: 570 LKR 572
           + +
Sbjct: 570 INQ 572

BLAST of Lsi04G008320 vs. TAIR 10
Match: AT5G45113.1 (mitochondrial transcription termination factor-related / mTERF-related )

HSP 1 Score: 291.2 bits (744), Expect = 1.9e-78
Identity = 158/412 (38.35%), Postives = 241/412 (58.50%), Query Frame = 0

Query: 160 LLENYHVLRNYGIQRNKIGKIYKEATQIFQYDYGVLLSKLKAYEELGLSQATVVKFVVCS 219
           + EN+HVL  YGI R+KIG++YKEA +IF Y+ GVL SKL+ YE L L +A V+K V C 
Sbjct: 1   MFENFHVLCYYGIPRDKIGRLYKEAREIFVYENGVLASKLEPYEILVLRKAIVIKLVTCC 60

Query: 220 PYLLVGGVNDGFAQVLDKLKNIGFESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEK 279
           P LLVGG++  F  V++KLK +     W+   L    +YNW++IL  +   +++G   +K
Sbjct: 61  PLLLVGGIDCEFVSVVNKLKGLNLGCDWLARYLSVRKTYNWRRILETMELLEKVGFKEKK 120

Query: 280 LADLISQHPDFLFEDSGSKSLSLIGLLLKMGSSMIQICSVFLQFPQIRVGKFVSNMRLCL 339
           L++L+  +PD + E SG+K+  +     K+G  M +I  + +   ++ + K V  +   L
Sbjct: 121 LSNLLKAYPDLVGETSGNKAYIMFEKFHKVGLQMNEIDKLLIDNSEMLLEKSVKRILEAL 180

Query: 340 LFFNEIDMGVQEIGYLFRSHPLLLGSCTLKKTNSLLANLNVGKKRLCQFILENPEELKNW 399
            F   I +  Q +    + H   + S +L    ++   L + +  LCQ I E P  L + 
Sbjct: 181 KFLKCIRIEKQFVVRFLQCHMKHICSSSLLVPRAVWNRLKIRRDELCQIIKEEPLRLFSI 240

Query: 400 KLGTRVPPLPDTGEVIRSKQQKTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIV 459
              T    + +   +     +KT FLLKLG  ENS EM +ALK F+G+G ELQERFDC V
Sbjct: 241 ASKTNKGRI-ELDSLDSRNAEKTTFLLKLGYVENSDEMVRALKKFQGRGDELQERFDCFV 300

Query: 460 EAGIDEKAVYKMILVSPQILNQTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQRVT 519
           +AG+D   V +++  +P ILN+ KDIIE+KI  L++ L YP+ S++  P+YL Y+ +R+ 
Sbjct: 301 KAGLDYNVVSQLVKRAPHILNRPKDIIEKKIIMLIDYLVYPIESVIESPTYLCYSMKRIH 360

Query: 520 LRFSMYSWLKEQGTADPMLALSTIVACSENTFLRQYVNHHPRGMEVWQNLKR 572
            RF+MY WL+E+    P L L T+V  S    +  +VN HP G   W+N+K+
Sbjct: 361 QRFTMYIWLRERDAVIPRLTLGTVVGISNTLIVPYFVNTHPEGPATWENIKK 411

BLAST of Lsi04G008320 vs. TAIR 10
Match: AT3G60400.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.7e-52
Identity = 147/517 (28.43%), Postives = 260/517 (50.29%), Query Frame = 0

Query: 69  EAQAALLEYLHSTRGIQHVDAENMSRNSPIFLEELLGRVEHE-GDIARSITRFLRYHPIN 128
           +AQ A+ +YLH+TR + +  AE ++ N+ + +  L+ +++      ++S+ + L YHPIN
Sbjct: 36  KAQQAITDYLHTTRSLSYTHAEQIASNASVSIRNLILKLDFSVPTFSKSLRKHLSYHPIN 95

Query: 129 EFEPFFESVGLQPAEYNAFLPRNLMFLSDDDLLLENYHVLRNYGIQRNKIGKIYKEATQI 188
           EFE FFES+G+  +E + FLP    F S+D  +L+    L  +G   NK+GK+YKE   +
Sbjct: 96  EFEFFFESIGIDYSEVSEFLPEKKFFFSEDRTVLDAAFALSGFGFPWNKLGKLYKEERLV 155

Query: 189 FQYDYGVLLSKLKAYEELGLSQATVVKFVVCSPYLLVGG--VNDGFAQVLDKLKNI--GF 248
           F    G + S+L  ++++G S   V+   +  P  L GG  +      +  KLK +   F
Sbjct: 156 FVQRPGEIESRLLKFKDIGFSTVAVIGTCLAIPRTLCGGGELGSEIRCLFVKLKRLFDEF 215

Query: 249 ESSWVEEQLIDGNSYNWKQILGLLFWFKQMGCCNEKLADLISQHPDFLFEDSGSKSLSLI 308
           +S  + E+ +D    +W  +   +  F  +GC NE++ +L+ ++     E S    ++  
Sbjct: 216 DSHHLFEENVD----SWLAVSRKIRIFYDLGCENEEMWELMCRNKSLFLEYSEEALMNKA 275

Query: 309 GLLLKMGSSMIQICSVFLQFPQIR---VGKFVSNMRLCLLFF----NEIDMGVQEIGYLF 368
           G   + G S      + L+ P I    + K V ++   L  F    +E+D   Q+  Y+F
Sbjct: 276 GYFCRFGVSKEDAALLILRNPAIMNFDLEKPVISVTGMLKHFGLRQDEVDAVAQKYPYVF 335

Query: 369 -----RSHPLLLGSCTL-KKTNSLLANLNVGKKRLCQFILENPEELKNWKLGTRVPPLPD 428
                ++ P +L +  L ++   +L N N     L  + L +P+E    +    +  L +
Sbjct: 336 GRNQLKNLPYVLRAIDLHERIFDILKNGN--HHLLASYTLMDPDEDLEREYQEGLEELQN 395

Query: 429 TGEVIRSKQQKTQFLLKLGLEENSSEMKKALKVFRGKGAELQERFDCIVEAGIDEKAVYK 488
           +    R   QK  FL ++G  EN   M K L+   G   EL +RF  ++ +GI    +  
Sbjct: 396 S-RTKRHNIQKLDFLHEIGFGENGITM-KVLQHVHGTAVELHDRFQILLNSGIIFSKICM 455

Query: 489 MILVSPQILNQTKDIIEEKIDFLVNNLGYPVSSLVNFPSYLSYTTQ-RVTLRFSMYSWLK 548
           +I  +P+ILNQ    I++K+ FL   +G  +  L  FP+YL +  + R++ RF  + WL 
Sbjct: 456 LIRSAPKILNQKPHSIQDKLRFLCGEMGDSLDYLEVFPAYLCFDLENRISPRFRFHKWLV 515

Query: 549 EQGTADPMLALSTIVACSENTFLRQYVNHHPRGMEVW 567
           E+G ++   ++++IVA SE  F+ +    HP   + W
Sbjct: 516 EKGFSEKSYSIASIVATSEKAFIARLYGIHPAIPKHW 544

BLAST of Lsi04G008320 vs. TAIR 10
Match: AT1G74120.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-09
Identity = 32/127 (25.20%), Postives = 66/127 (51.97%), Query Frame = 0

Query: 448 GAELQERFDCIVEAGIDEKAVYKMILVSPQILNQTKDIIEEKIDFLVNNLGYPVSSLVNF 507
           G E++ R DC+ + G+  +  +K++   P+++    + IE+KI+FL N +G+ ++ L + 
Sbjct: 282 GFEVKLRVDCLCKYGLIRRDAFKVVWKEPRVILYEIEDIEKKIEFLTNRMGFHINCLADV 341

Query: 508 PSYLSYTTQR-VTLRFSMYSWLKEQGTADPMLALSTIVACSENTFLRQYVNHHPRGMEVW 567
           P YL    Q+ +  R+++  +LK +G     + L  ++  S   F   YV  +P    ++
Sbjct: 342 PEYLGVNLQKQIVPRYNVIDYLKLKGGLGCDIGLKGLIKPSMKRFYNLYVMPYPECERIF 401

Query: 568 QNLKREI 574
              K  +
Sbjct: 402 GKRKENV 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2192.5e-5128.43Transcription termination factor MTEF18, mitochondrial OS=Arabidopsis thaliana O... [more]
Q9C6A12.0e-0825.20Transcription termination factor MTERF15, mitochondrial OS=Arabidopsis thaliana ... [more]
Match NameE-valueIdentityDescription
A0A6J1DXM21.6e-28785.34transcription termination factor MTEF18, mitochondrial-like OS=Momordica charant... [more]
A0A6J1HNT92.8e-28485.57transcription termination factor MTEF18, mitochondrial-like OS=Cucurbita maxima ... [more]
A0A6J1EEL82.3e-27883.85transcription termination factor MTEF18, mitochondrial-like isoform X1 OS=Cucurb... [more]
A0A5D3DAD94.8e-27682.56Transcription termination factor MTEF18 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S4DWM24.8e-27682.56transcription termination factor MTEF18, mitochondrial-like isoform X1 OS=Cucumi... [more]
Match NameE-valueIdentityDescription
XP_038891122.14.2e-29088.24transcription termination factor MTEF18, mitochondrial-like [Benincasa hispida][more]
XP_022158597.13.3e-28785.34transcription termination factor MTEF18, mitochondrial-like [Momordica charantia... [more]
XP_038882474.15.6e-28784.91transcription termination factor MTEF18, mitochondrial-like [Benincasa hispida][more]
XP_022966246.15.8e-28485.57transcription termination factor MTEF18, mitochondrial-like [Cucurbita maxima] >... [more]
XP_022925228.14.8e-27883.85transcription termination factor MTEF18, mitochondrial-like isoform X1 [Cucurbit... [more]
Match NameE-valueIdentityDescription
AT5G06810.11.0e-14548.11Mitochondrial transcription termination factor family protein [more]
AT4G19650.15.1e-12145.13Mitochondrial transcription termination factor family protein [more]
AT5G45113.11.9e-7838.35mitochondrial transcription termination factor-related / mTERF-related [more]
AT3G60400.11.7e-5228.43Mitochondrial transcription termination factor family protein [more]
AT1G74120.11.4e-0925.20Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 213..244
e-value: 130.0
score: 10.7
coord: 469..500
e-value: 2.3
score: 17.2
coord: 178..208
e-value: 17.0
score: 14.3
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 270..401
e-value: 2.3E-9
score: 36.6
coord: 419..552
e-value: 9.7E-15
score: 54.3
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 49..570
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 115..254
e-value: 9.5E-11
score: 43.3
coord: 297..571
e-value: 1.8E-50
score: 173.6
NoneNo IPR availablePANTHERPTHR13068:SF38TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEINcoord: 49..570

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G008320.1Lsi04G008320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0032502 developmental process
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009507 chloroplast
molecular_function GO:0003690 double-stranded DNA binding