Lsi04G008060 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G008060
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCytokinin dehydrogenase
Locationchr04: 7657324 .. 7659926 (-)
RNA-Seq ExpressionLsi04G008060
SyntenyLsi04G008060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGAAAGGTTCTCAAACTGTTTGTCTTTCGTGAAAAAGGTTGGGTTTTTAACTGTTTTAAAAAAGTACAAAGATTTATGAAATCTTTCTTTCCGGTGATTTTCCTCTTCAACTTGGCATATTACTGATGACTTCTCTTAACGATTGAACAAAAAGGAAATCCCGAAACTCTTTCCCTAAGTAGGCAGTCTTCATACGTATTCTCCCAACTGAAGTCTAGTTGACTCAGCCACTATAATCTTAATCTCCCTTTAAATCCACCTCCATTTCAGCATTTTCTAGGCCCTCCAACACCTCTTCTGCTTTTTCTCCTTAGAAATAACAAGAACATGTTACCTCTCCTTTCATCTTTAACTTCAGAGACCTGTACCCAAAGAAAAATACGAAATCCACCAGTTAGCTTCCTCAGACAAAACAACATGCTTTCCATTCGAAGCTTCATGCTTTTCTTCCTGACTTTTATTGCTGTTAGGATGAATCTATGCTTCACAGGCATCCCTTCTTCACTCAAGATGCTTTCCATCAACGGGCATTTTGAATTTGATAAAGTGCAATTTGCCGCAAGAGATTTTGGTAATAGATATCAATTCTACCCTGCAGCAATACTGCATCCATCATCAGTTTCTGATATTGCCATGACTATAAAGCATATTTGGAAGATGGGACCTCGTTCACAGCTCACAGTTGCAGCTAGAGGCCATGGCCACTCACTTCATGGCCAAGCACAGGCCCACCAAGGAGTTGTGATTAACATGGAATCACTTCAGGGCCCTCAAATGCAGGTTCATACCAGAAACTTCACATACATCGATGTTTCGGGAGGGGAGTTATGGATAAATATCCTGCACGAGAGCTTGAAACACGGATTAACACCAAAATCATGGACAGACTACCTACATCTAACTGTTGGAGGTACCTTATCCAATGCAGGGATCAGTGGACAGGCATTTCGGCATGGCCCTCAGATCAGCAACGTTCATCAGCTGGAGGTTGTAACAGGTTCGTTGCCATTTACATTATACTATTATATACAAGAGCATGTCTCTGGCGCCTGTGGGCATGTAAAATGTTAAGAGTAACGGGGAGGGAAATCCATGCAGTTAATAATTGCACTATGAATGGTAAGCAAAAGTTGGGGAAGAACATGAAAAGATAAATGGCAAACTATTGACTTGTCTTAACGCCATTTCCCATAGGTGGTATTTTAAGTTGCCAAGTTCTCTTTACTATTTCGTCACCCAACCATAAAGTCATTAAGGACAACTGAAAGTTATCAAAAAAAAATTAATTGCTACCAGGCATATCATTATGTTAACTGACAAATGGAGTGAAACTGAATTCAGGAAAAGGAGATGTGGTGAACTGTTCAAAGGAACAGAACAGTGACCTCTTTTACAGTGTTCTTGGAGGATTAGGCCAGTTTGGAATTATCACACGAGCAAAAATTCTACTTGAACCAGCGCCAACAATGGTAAGACTATTGTTTGTAAAGGCAACTTTGAAGATTTCGCCTAGAGTAACTACAAAGGATTGTTCTAACACAGTTCTTCTATCTTTGACAGGTAAAATGGATAAGAGTGTTATACTTAGACTTCGCTACTTTTTCCAGAGAGCAGGAGCTCTTGATATCTGCAGAAAAGACATTCGACTACATTGAAGGATTTGTTATCATAAACAGAACTGGTCTTTTGAATAATTGGAGATCATCATTCAATCCACAAGACCCAGTTCAAGCTAGCCAGTTCAAGTCTGATGGCAAAATTCTTTACTGCCTAGAATTGGCAAAGTACTTTCACCACAACGAGGACAACATTATTGATCAGGTAAAGTACCCACACTAAATCTATGTAAAAATGATCAGATAAATGGCAGATAATACATGGAAAAGGATATTTGATTCAGAGGATCTCATAGGCGCATGCTTTTACTATGCAGGAAGTCACGCACCTGTTGTCTCAGTTGAGTTATATCCCATCAACACTTTTCATATCAGAAGTTACATATGTGGAATTCCTTGACAGAGTTCAAGTATCAGCCGTCAAATTACAGTCAAAAGGCTTGTGGGAAGTCCCACACCCATGGCTGAATCTCCTTATTCCAAAAAGCAAAATAAAAAAATTTGCAGAAGGGGTTTTCGGCAATATCCTGAAGGAAACAAGCAACGGCCCTGTCCTAATCTATCCTGTTAACAAATCAAAGTAAGGGATTATCTAACCAAGTTACTGGCTAAAATATTCTTGCAATATGTATTATGTTAATTCAAGTATTTCTGACTTTCAGGTGGGACAATAGAACTTCTGTAGTTATTCCAGAGGAGGAAATTTTCTACCTGGTCGCGTTTCTAACCTCTGCAGTTCCCTCTTCTAAAGGAAACGACAGCTTAGAATACATCTTAACTCAGAACATGAGAATTCTGGAATTCTGTAAAACAGCCAATCTAGGAGCGAAACAATATTTGCCACATTACACTACAAGGAACGAATGGCAGGCCCATTTTGGCCCAATGTGGGAAACATATGTACAGAGAAAAACAGCTTATGACCCTTTGGCAATACTTGCTCCAGGTCAAAGAATATTCCAAAAGGCAAAATCCTTGTCATGA

mRNA sequence

GAGAGAAAGGTTCTCAAACTGTTTGTCTTTCGTGAAAAAGGTTGGGTTTTTAACTGTTTTAAAAAAGTACAAAGATTTATGAAATCTTTCTTTCCGGTGATTTTCCTCTTCAACTTGGCATATTACTGATGACTTCTCTTAACGATTGAACAAAAAGGAAATCCCGAAACTCTTTCCCTAAGTAGGCAGTCTTCATACGTATTCTCCCAACTGAAGTCTAGTTGACTCAGCCACTATAATCTTAATCTCCCTTTAAATCCACCTCCATTTCAGCATTTTCTAGGCCCTCCAACACCTCTTCTGCTTTTTCTCCTTAGAAATAACAAGAACATGTTACCTCTCCTTTCATCTTTAACTTCAGAGACCTGTACCCAAAGAAAAATACGAAATCCACCAGTTAGCTTCCTCAGACAAAACAACATGCTTTCCATTCGAAGCTTCATGCTTTTCTTCCTGACTTTTATTGCTGTTAGGATGAATCTATGCTTCACAGGCATCCCTTCTTCACTCAAGATGCTTTCCATCAACGGGCATTTTGAATTTGATAAAGTGCAATTTGCCGCAAGAGATTTTGGTAATAGATATCAATTCTACCCTGCAGCAATACTGCATCCATCATCAGTTTCTGATATTGCCATGACTATAAAGCATATTTGGAAGATGGGACCTCGTTCACAGCTCACAGTTGCAGCTAGAGGCCATGGCCACTCACTTCATGGCCAAGCACAGGCCCACCAAGGAGTTGTGATTAACATGGAATCACTTCAGGGCCCTCAAATGCAGGTTCATACCAGAAACTTCACATACATCGATGTTTCGGGAGGGGAGTTATGGATAAATATCCTGCACGAGAGCTTGAAACACGGATTAACACCAAAATCATGGACAGACTACCTACATCTAACTGTTGGAGGTACCTTATCCAATGCAGGGATCAGTGGACAGGCATTTCGGCATGGCCCTCAGATCAGCAACGTTCATCAGCTGGAGGTTGTAACAGGAAAAGGAGATGTGGTGAACTGTTCAAAGGAACAGAACAGTGACCTCTTTTACAGTGTTCTTGGAGGATTAGGCCAGTTTGGAATTATCACACGAGCAAAAATTCTACTTGAACCAGCGCCAACAATGGTAAAATGGATAAGAGTGTTATACTTAGACTTCGCTACTTTTTCCAGAGAGCAGGAGCTCTTGATATCTGCAGAAAAGACATTCGACTACATTGAAGGATTTGTTATCATAAACAGAACTGGTCTTTTGAATAATTGGAGATCATCATTCAATCCACAAGACCCAGTTCAAGCTAGCCAGTTCAAGTCTGATGGCAAAATTCTTTACTGCCTAGAATTGGCAAAGTACTTTCACCACAACGAGGACAACATTATTGATCAGGAAGTCACGCACCTGTTGTCTCAGTTGAGTTATATCCCATCAACACTTTTCATATCAGAAGTTACATATGTGGAATTCCTTGACAGAGTTCAAGTATCAGCCGTCAAATTACAGTCAAAAGGCTTGTGGGAAGTCCCACACCCATGGCTGAATCTCCTTATTCCAAAAAGCAAAATAAAAAAATTTGCAGAAGGGGTTTTCGGCAATATCCTGAAGGAAACAAGCAACGGCCCTGTCCTAATCTATCCTGTTAACAAATCAAAGTGGGACAATAGAACTTCTGTAGTTATTCCAGAGGAGGAAATTTTCTACCTGGTCGCGTTTCTAACCTCTGCAGTTCCCTCTTCTAAAGGAAACGACAGCTTAGAATACATCTTAACTCAGAACATGAGAATTCTGGAATTCTGTAAAACAGCCAATCTAGGAGCGAAACAATATTTGCCACATTACACTACAAGGAACGAATGGCAGGCCCATTTTGGCCCAATGTGGGAAACATATGTACAGAGAAAAACAGCTTATGACCCTTTGGCAATACTTGCTCCAGGTCAAAGAATATTCCAAAAGGCAAAATCCTTGTCATGA

Coding sequence (CDS)

ATGTTACCTCTCCTTTCATCTTTAACTTCAGAGACCTGTACCCAAAGAAAAATACGAAATCCACCAGTTAGCTTCCTCAGACAAAACAACATGCTTTCCATTCGAAGCTTCATGCTTTTCTTCCTGACTTTTATTGCTGTTAGGATGAATCTATGCTTCACAGGCATCCCTTCTTCACTCAAGATGCTTTCCATCAACGGGCATTTTGAATTTGATAAAGTGCAATTTGCCGCAAGAGATTTTGGTAATAGATATCAATTCTACCCTGCAGCAATACTGCATCCATCATCAGTTTCTGATATTGCCATGACTATAAAGCATATTTGGAAGATGGGACCTCGTTCACAGCTCACAGTTGCAGCTAGAGGCCATGGCCACTCACTTCATGGCCAAGCACAGGCCCACCAAGGAGTTGTGATTAACATGGAATCACTTCAGGGCCCTCAAATGCAGGTTCATACCAGAAACTTCACATACATCGATGTTTCGGGAGGGGAGTTATGGATAAATATCCTGCACGAGAGCTTGAAACACGGATTAACACCAAAATCATGGACAGACTACCTACATCTAACTGTTGGAGGTACCTTATCCAATGCAGGGATCAGTGGACAGGCATTTCGGCATGGCCCTCAGATCAGCAACGTTCATCAGCTGGAGGTTGTAACAGGAAAAGGAGATGTGGTGAACTGTTCAAAGGAACAGAACAGTGACCTCTTTTACAGTGTTCTTGGAGGATTAGGCCAGTTTGGAATTATCACACGAGCAAAAATTCTACTTGAACCAGCGCCAACAATGGTAAAATGGATAAGAGTGTTATACTTAGACTTCGCTACTTTTTCCAGAGAGCAGGAGCTCTTGATATCTGCAGAAAAGACATTCGACTACATTGAAGGATTTGTTATCATAAACAGAACTGGTCTTTTGAATAATTGGAGATCATCATTCAATCCACAAGACCCAGTTCAAGCTAGCCAGTTCAAGTCTGATGGCAAAATTCTTTACTGCCTAGAATTGGCAAAGTACTTTCACCACAACGAGGACAACATTATTGATCAGGAAGTCACGCACCTGTTGTCTCAGTTGAGTTATATCCCATCAACACTTTTCATATCAGAAGTTACATATGTGGAATTCCTTGACAGAGTTCAAGTATCAGCCGTCAAATTACAGTCAAAAGGCTTGTGGGAAGTCCCACACCCATGGCTGAATCTCCTTATTCCAAAAAGCAAAATAAAAAAATTTGCAGAAGGGGTTTTCGGCAATATCCTGAAGGAAACAAGCAACGGCCCTGTCCTAATCTATCCTGTTAACAAATCAAAGTGGGACAATAGAACTTCTGTAGTTATTCCAGAGGAGGAAATTTTCTACCTGGTCGCGTTTCTAACCTCTGCAGTTCCCTCTTCTAAAGGAAACGACAGCTTAGAATACATCTTAACTCAGAACATGAGAATTCTGGAATTCTGTAAAACAGCCAATCTAGGAGCGAAACAATATTTGCCACATTACACTACAAGGAACGAATGGCAGGCCCATTTTGGCCCAATGTGGGAAACATATGTACAGAGAAAAACAGCTTATGACCCTTTGGCAATACTTGCTCCAGGTCAAAGAATATTCCAAAAGGCAAAATCCTTGTCATGA

Protein sequence

MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSLKMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGFVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIFQKAKSLS
Homology
BLAST of Lsi04G008060 vs. ExPASy Swiss-Prot
Match: Q9LY71 (Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2)

HSP 1 Score: 750.0 bits (1935), Expect = 1.9e-215
Identity = 363/527 (68.88%), Postives = 427/527 (81.02%), Query Frame = 0

Query: 24  SFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSLKMLSINGHFEFDKVQFAARDFGN 83
           S LR+  ML +RSF +  L+ IA ++  CF+   SSLK L + GH EF+ V  A++DFGN
Sbjct: 7   SLLRKRTMLIVRSFTILLLSCIAFKLACCFSSSISSLKALPLVGHLEFEHVHHASKDFGN 66

Query: 84  RYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLHGQAQAHQGVVINME 143
           RYQ  P A+LHP SVSDIA TI+HIW MG  SQLTVAARG GHSL GQAQ   G+VI+ME
Sbjct: 67  RYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHSLQGQAQTRHGIVIHME 126

Query: 144 SLQGPQMQVHTRN--FTYIDVSGGELWINILHESLKHGLTPKSWTDYLHLTVGGTLSNAG 203
           SL   ++QV++ +    Y+DVSGGELWINILHE+LK+GL PKSWTDYLHLTVGGTLSNAG
Sbjct: 127 SLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSWTDYLHLTVGGTLSNAG 186

Query: 204 ISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLFYSVLGGLGQFGIITRAKILLE 263
           ISGQAFRHGPQISNVHQLE+VTGKG+++NC+K QNSDLF  VLGGLGQFGIITRA+I LE
Sbjct: 187 ISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLGGLGQFGIITRARIALE 246

Query: 264 PAPTMVKWIRVLYLDFATFSREQELLISAE-KTFDYIEGFVIINRTGLLNNWRSSFNPQD 323
           PAPTMVKWIRVLYLDFA F+++QE LISA+   FDYIEGFVIINRTGLLN+WR SF  ++
Sbjct: 247 PAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIINRTGLLNSWRLSFTAEE 306

Query: 324 PVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLSQLSYIPSTLFISEVTYVEFL 383
           P++ASQFK DG+ LYCLELAKY   +  ++I+QEV   LS+LSY+ STLF +EV Y  FL
Sbjct: 307 PLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELSYVTSTLFTTEVAYEAFL 366

Query: 384 DRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSNGPVLIYPVNKS 443
           DRV VS VKL+SKG WEVPHPWLNLL+P+SKI +FA GVFGNIL +TSNGPV++YPVNKS
Sbjct: 367 DRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNILTDTSNGPVIVYPVNKS 426

Query: 444 KWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILTQNMRILEFCKTANLGAKQYL 503
           KWDN+TS V PEEE+FYLVA LTSA P S G D +E IL +N RILEF + A +G KQYL
Sbjct: 427 KWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNRRILEFSEEAGIGLKQYL 486

Query: 504 PHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIFQKAKSLS 548
           PHYTTR EW++HFG  W  +V+RK+ YDPLAILAPG RIFQKA S S
Sbjct: 487 PHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKAVSYS 533

BLAST of Lsi04G008060 vs. ExPASy Swiss-Prot
Match: O22213 (Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.5e-207
Identity = 352/533 (66.04%), Postives = 415/533 (77.86%), Query Frame = 0

Query: 25  FLRQNNMLSIRSFMLFFLTFIAVRMNLCF---------------TGIPSSLKMLSINGHF 84
           F RQNN   +  FM+  L+ I  R NLC                + I SSL  L + G+ 
Sbjct: 9   FHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRSSLVSLDLEGYI 68

Query: 85  EFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLH 144
            FD V   A+DFGNRYQ  P AILHP SV DI+  +KHI  +G  S LTVAARGHGHSL 
Sbjct: 69  SFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSLQ 128

Query: 145 GQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGLTPKSWTDYL 204
           GQA AHQGVVI MESL+ P ++++     Y+DVSGGE+WINIL E+LK+GL+PKSWTDYL
Sbjct: 129 GQALAHQGVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWTDYL 188

Query: 205 HLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLFYSVLGGLGQ 264
           HLTVGGTLSNAGISGQAF+HGPQI+NV+QLE+VTGKG+VV CS+++NS+LF+SVLGGLGQ
Sbjct: 189 HLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLGQ 248

Query: 265 FGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGFVIINRTGLL 324
           FGIITRA+I LEPAP MVKWIRVLY DF+ FSR+QE LIS EKTFDY+EGFVIINRT LL
Sbjct: 249 FGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDLL 308

Query: 325 NNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLSQLSYIPSTL 384
           NNWRSSF+P D  QAS+FKSDGK LYCLE+ KYF+  E + +DQE   LLS+L+YIPSTL
Sbjct: 309 NNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPSTL 368

Query: 385 FISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSN 444
           F SEV Y+EFLDRV ++  KL++KGLWEVPHPWLNLLIPKS I +FA  VF NIL   +N
Sbjct: 369 FSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNNN 428

Query: 445 GPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILTQNMRILEFC 504
           GP+LIYPVN+SKW   TS++ P E+IFYLVAFL SAVP+S G + LEY+L QN R++ FC
Sbjct: 429 GPILIYPVNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNFC 488

Query: 505 KTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIFQK 543
             ANL  KQYLPHY T+ EW++HFG  WET+ QRK AYDPLAILAPGQRIFQK
Sbjct: 489 AAANLNVKQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQK 541

BLAST of Lsi04G008060 vs. ExPASy Swiss-Prot
Match: Q75K78 (Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 6.2e-206
Identity = 331/490 (67.55%), Postives = 417/490 (85.10%), Query Frame = 0

Query: 56  IPSSLKMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRS 115
           +PSSL+ L+++GHF F  V  AA+DFGN   F P A+LHP SV+DIA TI+H++ MG  S
Sbjct: 30  VPSSLEELTLDGHFSFHDVSAAAQDFGNLSSFPPVAVLHPGSVADIATTIRHVFLMGEHS 89

Query: 116 QLTVAARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHES 175
            LTVAARGHGHSL+GQ+QA +G++I+MESLQ   M+V+     Y+D SGGELWIN+LHE+
Sbjct: 90  TLTVAARGHGHSLYGQSQAAEGIIISMESLQSNTMRVNPGVSPYVDASGGELWINVLHET 149

Query: 176 LKHGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQ 235
           LK+GL PKSWTDYLHLTVGGTLSNAG+SGQ FRHGPQISNV++LE+VTG+GDV+ CS EQ
Sbjct: 150 LKYGLAPKSWTDYLHLTVGGTLSNAGVSGQTFRHGPQISNVNELEIVTGRGDVITCSPEQ 209

Query: 236 NSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFD 295
           NSDLF++ LGGLGQFG+ITRA+I LEPAP MV+W+RVLYLDF +F+ +QE+LISAEKTFD
Sbjct: 210 NSDLFHAALGGLGQFGVITRARIPLEPAPKMVRWLRVLYLDFTSFTEDQEMLISAEKTFD 269

Query: 296 YIEGFVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEV 355
           YIEGFVIINRTG+LNNWRSSFNPQDPV++SQF+SDGK+L+CLE+ K F+ +E ++++QEV
Sbjct: 270 YIEGFVIINRTGILNNWRSSFNPQDPVRSSQFESDGKVLFCLEMTKNFNPDEADVMEQEV 329

Query: 356 THLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKF 415
             LLSQL Y+PS+LF ++VTY+EFLDRV  S +KL++KG+WEVPHPWLN++IP+S I KF
Sbjct: 330 NTLLSQLRYMPSSLFHTDVTYIEFLDRVHSSEMKLRAKGMWEVPHPWLNIIIPRSMIHKF 389

Query: 416 AEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSL 475
           A+ VFG ILK+++NGP+L+YPVNKS+WDNRTSVVIP+EE+FYLVAFL+SA+    G  ++
Sbjct: 390 AKEVFGKILKDSNNGPILLYPVNKSRWDNRTSVVIPDEEVFYLVAFLSSAL----GPHNI 449

Query: 476 EYILTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAP 535
           ++ L  N RI+EF   A +G KQYLP+YTT  EWQ+HFG  W+T+ QRK AYDPLAILAP
Sbjct: 450 KHTLDLNYRIIEFSDKAGIGVKQYLPNYTTEQEWQSHFGARWDTFQQRKKAYDPLAILAP 509

Query: 536 GQRIFQKAKS 546
           GQRIFQKA +
Sbjct: 510 GQRIFQKASA 515

BLAST of Lsi04G008060 vs. ExPASy Swiss-Prot
Match: Q5JLP4 (Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 4.3e-199
Identity = 326/491 (66.40%), Postives = 405/491 (82.48%), Query Frame = 0

Query: 56  IPSSLKMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRS 115
           + +SL  L ++GHF FD    AARDFGNR    PAA+LHP SVSD+A T++ ++++G  S
Sbjct: 34  VVASLGALRLDGHFSFDDAHAAARDFGNRCSLLPAAVLHPGSVSDVAATVRRVFQLGRSS 93

Query: 116 QLTVAARGHGHSLHGQAQAHQGVVINMESLQGP---QMQVHTRNFTYIDVSGGELWINIL 175
            LTVAARGHGHSL GQ+QA  G+V+ MESL       ++VH     ++D  GGELWIN+L
Sbjct: 94  PLTVAARGHGHSLLGQSQAAGGIVVKMESLAAAAARAVRVHGGASPHVDAPGGELWINVL 153

Query: 176 HESLKHGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS 235
           HE+LKHGL P+SWTDYLHLTVGGTLSNAG+SGQAFRHGPQ+SNV+QLE+VTG+G+VV CS
Sbjct: 154 HETLKHGLAPRSWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNVNQLEIVTGRGEVVTCS 213

Query: 236 KEQNSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEK 295
            E NSDLFY+ LGGLGQFGIITRA+I LEPAP MV+WIRVLY DF TF+ +QE LI++EK
Sbjct: 214 HEVNSDLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSDFETFTEDQEKLIASEK 273

Query: 296 TFDYIEGFVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIID 355
           TFDYIEGFVIINRTG+LNNWR+SF PQDPVQASQF+SDG++LYCLEL   F+H+E +I++
Sbjct: 274 TFDYIEGFVIINRTGILNNWRTSFKPQDPVQASQFQSDGRVLYCLELTMNFNHDEADIME 333

Query: 356 QEVTHLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKI 415
           QEV  LLS+L YI STLF ++VTY+EFLDRV  S +KL+++GLWEVPHPWLNLLIP+S +
Sbjct: 334 QEVGALLSRLRYISSTLFYTDVTYLEFLDRVHTSELKLRAQGLWEVPHPWLNLLIPRSTV 393

Query: 416 KKFAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGN 475
            KFA+ VFG ILK+++NGP+L+YPVN++KWDNRTSVVIP+EEIFYLV FL+SA PSS G+
Sbjct: 394 HKFAKEVFGKILKDSNNGPILLYPVNRTKWDNRTSVVIPDEEIFYLVGFLSSA-PSSSGH 453

Query: 476 DSLEYILTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAI 535
            S+E+ +  N +I++FC+   +G KQYL  YTT+ +W+AHFG  WET+ +RK  YDPLAI
Sbjct: 454 GSVEHAMNLNNKIVDFCEKNGVGMKQYLAPYTTQKQWKAHFGARWETFERRKHTYDPLAI 513

Query: 536 LAPGQRIFQKA 544
           LAPGQRIF KA
Sbjct: 514 LAPGQRIFPKA 523

BLAST of Lsi04G008060 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 2.3e-152
Identity = 263/487 (54.00%), Postives = 351/487 (72.07%), Query Frame = 0

Query: 63  LSINGHFEFDKVQFA--ARDFGN-RYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTV 122
           + ++GHF       A  + DFG  +    P A+LHPSS  D+A  ++  +  G  +   V
Sbjct: 38  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 97

Query: 123 AARGHGHSLHGQAQA-HQGVVINM-ESLQGPQMQVHTRNFTYIDVSGGELWINILHESLK 182
           +ARGHGHS++GQA A   GVV+ M   + G    +   +  Y+DV GGELW+++L ++L+
Sbjct: 98  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEMYVDVWGGELWVDVLKKTLE 157

Query: 183 HGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNS 242
           HGL PKSWTDYL+LTVGGTLSNAGISGQAF HGPQISNV +L+VVTGKG+V+ CS+E+N+
Sbjct: 158 HGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENT 217

Query: 243 DLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLIS--AEKTFD 302
            LF+ VLGGLGQFGIITRA+I LEPAP  V+WIRVLY  F  F+ +QE LIS   +  FD
Sbjct: 218 RLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFD 277

Query: 303 YIEGFVIINRTGLLNNWRSS-FNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQE 362
           Y+EGFVI++  GL+NNWRSS F+P++PV+ S   S+G +LYCLE+ K +H ++  I+DQE
Sbjct: 278 YVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQE 337

Query: 363 VTHLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKK 422
           V  L+ +L++IP+++F +++ YV+FLDRV  + +KL+SK LWEVPHPWLNL +PKS+I  
Sbjct: 338 VEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISD 397

Query: 423 FAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDS 482
           F +GVF  IL   ++GP+LIYP+NK KWD R+S V P+EE+FYLVA L SA+   +    
Sbjct: 398 FDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQK 457

Query: 483 LEYILTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILA 542
           LEY+  QN RILEFC+ A +  KQYLPH+ T+ EW AHFG  W+ +   K  +DP  ILA
Sbjct: 458 LEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILA 517

BLAST of Lsi04G008060 vs. ExPASy TrEMBL
Match: A0A1S3BD71 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488756 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.1e-309
Identity = 523/547 (95.61%), Postives = 535/547 (97.81%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLPLLSSLTSETCTQRK R PPVSFLRQNNMLSIRSF++FFL+FIA RMNLCFTGIPSSL
Sbjct: 1   MLPLLSSLTSETCTQRKTRYPPVSFLRQNNMLSIRSFVIFFLSFIAFRMNLCFTGIPSSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           KMLSI+GHFEFD+VQFAARDFGNRYQF+PAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA
Sbjct: 61  KMLSIDGHFEFDQVQFAARDFGNRYQFFPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGL 180
           ARGHGHSLHGQAQAHQGVVINM+SLQGPQMQVHTRNFTYIDVSGGELWINILHESLK+GL
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMKSLQGPQMQVHTRNFTYIDVSGGELWINILHESLKYGL 180

Query: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240
           TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGDVVNCSKEQNSDLF
Sbjct: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGDVVNCSKEQNSDLF 240

Query: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGF 300
           YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDF TFSREQELLISA+ TFDYIEGF
Sbjct: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFTTFSREQELLISAQNTFDYIEGF 300

Query: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLS 360
           VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHH E NIIDQEV  LLS
Sbjct: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHTEGNIIDQEVMRLLS 360

Query: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420
           QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF
Sbjct: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420

Query: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILT 480
           GNILKETSNGPVLIYPVNKSKWDNRTSV+IPEEEIFYLVAFLTSAVPSS+GNDSLEYILT
Sbjct: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVIIPEEEIFYLVAFLTSAVPSSRGNDSLEYILT 480

Query: 481 QNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540
           QNMRILEFCKTANLG KQYLPHYTTR EWQAHFGPMWETYVQRK AYDPLAILAPGQRIF
Sbjct: 481 QNMRILEFCKTANLGVKQYLPHYTTRKEWQAHFGPMWETYVQRKAAYDPLAILAPGQRIF 540

Query: 541 QKAKSLS 548
           QKAKSLS
Sbjct: 541 QKAKSLS 547

BLAST of Lsi04G008060 vs. ExPASy TrEMBL
Match: A0A6J1CFB1 (Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111010228 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 6.7e-304
Identity = 511/547 (93.42%), Postives = 532/547 (97.26%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLPLLSSLTSETCTQRK+R PPVSFLRQNNMLSIRSFM+ FL+ IA+RMNLCF GIPSSL
Sbjct: 1   MLPLLSSLTSETCTQRKLRYPPVSFLRQNNMLSIRSFMILFLSCIALRMNLCFNGIPSSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           KMLSINGHFEFDKVQ+AARDFGNRYQ+YPAAILHPSSVSDIAMTIKHIWKMGPRS+LTVA
Sbjct: 61  KMLSINGHFEFDKVQYAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPRSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGL 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTR+FTYIDV GGELWINILHESLK+GL
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRDFTYIDVPGGELWINILHESLKYGL 180

Query: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240
           TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF
Sbjct: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240

Query: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGF 300
           YSVLGGLGQFG+ITRAKILLEPAP  VKWIRVLYLDF TFSREQELLISAE TF+YIEGF
Sbjct: 241 YSVLGGLGQFGVITRAKILLEPAPAKVKWIRVLYLDFTTFSREQELLISAENTFNYIEGF 300

Query: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLS 360
           VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYF+HNE NI+DQEV  LLS
Sbjct: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFNHNEGNIVDQEVKSLLS 360

Query: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420
           +LSYIPSTLFISEVTYVEFLDRV VSAVKLQSKGLWEVPHPWLNLL+PKSKI+KF+EGVF
Sbjct: 361 RLSYIPSTLFISEVTYVEFLDRVHVSAVKLQSKGLWEVPHPWLNLLVPKSKIEKFSEGVF 420

Query: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILT 480
           GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEE+IFYL+AFLTSAVPSSKGND LEYILT
Sbjct: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEDIFYLIAFLTSAVPSSKGNDGLEYILT 480

Query: 481 QNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540
           QNMRILEFCKTANLGAKQYLPHYTT+NEW+AHFGPMWETYVQRKTAYDPLAILAPGQRIF
Sbjct: 481 QNMRILEFCKTANLGAKQYLPHYTTKNEWKAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540

Query: 541 QKAKSLS 548
           QKAK LS
Sbjct: 541 QKAKPLS 547

BLAST of Lsi04G008060 vs. ExPASy TrEMBL
Match: A0A6J1FSN0 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111446933 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.2e-294
Identity = 501/549 (91.26%), Postives = 525/549 (95.63%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLP+LSSLTSETC+QRK+R PPVSFL+Q NML IRSFM+ FL+ IAVRMNLCFTGIP SL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           +ML INGHFEFDKV+FAARDFGNRYQ+YPAAILHPSSVSDIAMTIKHIWKMGP S+LTVA
Sbjct: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQ-VHTRNFTYIDVSGGELWINILHESLKHG 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQ VH R+FTYIDVSGGELWINIL ESLK+G
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHNRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLY DF TF+REQELLIS E TFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNED-NIIDQEVTHL 360
           FVIINRTGLLNNWRSSF+PQDPVQASQFKS+GKILYCLELAKYFHHN+D N +DQEV  L
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360

Query: 361 LSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEG 420
           LS+LSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIK+FAEG
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKEFAEG 420

Query: 421 VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYI 480
           VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEE+IFYLVAFLTSAVP S+GNDSLEYI
Sbjct: 421 VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEDIFYLVAFLTSAVPCSRGNDSLEYI 480

Query: 481 LTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQR 540
           LTQN RILEFCKTANLGAKQYLPHYTTR EWQAHFGPMWETYV+RK AYDPLAILAPGQR
Sbjct: 481 LTQNTRILEFCKTANLGAKQYLPHYTTRKEWQAHFGPMWETYVERKMAYDPLAILAPGQR 540

Query: 541 IFQKAKSLS 548
           IF+KAK LS
Sbjct: 541 IFKKAKPLS 549

BLAST of Lsi04G008060 vs. ExPASy TrEMBL
Match: A0A6J1JHC8 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111485722 PE=3 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.1e-293
Identity = 499/549 (90.89%), Postives = 526/549 (95.81%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLP+LSSLTSETC+QRK+R PPVSFL+Q NMLSIRSFM+ FL+ IAVRMNLCFTGIP SL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLSIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           +ML INGHFEF+KV+FAARDFGNRYQ+YPAAILHPSSVSDIAMTIKHIWKMGP S+LTVA
Sbjct: 61  RMLYINGHFEFEKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQ-VHTRNFTYIDVSGGELWINILHESLKHG 180
           ARGHGHSLHGQAQAH+GVVINMESLQGPQMQ VHTR+FTYIDVSGGELWINIL ESLK+G
Sbjct: 121 ARGHGHSLHGQAQAHRGVVINMESLQGPQMQVVHTRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLY DF TF+REQELLIS E TFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNED-NIIDQEVTHL 360
           FVIINRTGLLNNWRSSF+PQDPVQASQFKS+GKILYCLELAKYFHHN+D N +D E+  L
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDLEIKRL 360

Query: 361 LSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEG 420
           LS+LSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIK+FAEG
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKEFAEG 420

Query: 421 VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYI 480
           VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEE+IFYLVAFLTSAVP S+GNDSLEYI
Sbjct: 421 VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEDIFYLVAFLTSAVPWSRGNDSLEYI 480

Query: 481 LTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQR 540
           LTQN RIL+FCKTANLGAKQYLPHYTTR EWQAHFGPMWETYV+RK AYDPLAILAPGQR
Sbjct: 481 LTQNTRILDFCKTANLGAKQYLPHYTTRKEWQAHFGPMWETYVERKRAYDPLAILAPGQR 540

Query: 541 IFQKAKSLS 548
           IF+KAK LS
Sbjct: 541 IFKKAKPLS 549

BLAST of Lsi04G008060 vs. ExPASy TrEMBL
Match: A0A5A7SXR4 (Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00430 PE=3 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.1e-293
Identity = 495/517 (95.74%), Postives = 507/517 (98.07%), Query Frame = 0

Query: 31  MLSIRSFMLFFLTFIAVRMNLCFTGIPSSLKMLSINGHFEFDKVQFAARDFGNRYQFYPA 90
           MLSIRSF++FFL+FIA RMNLCFTGIPSSLKMLSI+GHFEFD+VQFAARDFGNRYQF+PA
Sbjct: 1   MLSIRSFVIFFLSFIAFRMNLCFTGIPSSLKMLSIDGHFEFDQVQFAARDFGNRYQFFPA 60

Query: 91  AILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLHGQAQAHQGVVINMESLQGPQM 150
           AILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLHGQAQAHQGVVINM+SLQGPQM
Sbjct: 61  AILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLHGQAQAHQGVVINMKSLQGPQM 120

Query: 151 QVHTRNFTYIDVSGGELWINILHESLKHGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHG 210
           QVHTRNFTYIDVSGGELWINILHESLK+GLTPKSWTDYLHLTVGGTLSNAGISGQAFRHG
Sbjct: 121 QVHTRNFTYIDVSGGELWINILHESLKYGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHG 180

Query: 211 PQISNVHQLEVVTGKGDVVNCSKEQNSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWI 270
           PQISNVHQLE+VTGKGDVVNCSKEQNSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWI
Sbjct: 181 PQISNVHQLEIVTGKGDVVNCSKEQNSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWI 240

Query: 271 RVLYLDFATFSREQELLISAEKTFDYIEGFVIINRTGLLNNWRSSFNPQDPVQASQFKSD 330
           RVLYLDF TFSREQELLISA+ TFDYIEGFVIINRTGLLNNWRSSFNPQDPVQASQFKSD
Sbjct: 241 RVLYLDFTTFSREQELLISAQNTFDYIEGFVIINRTGLLNNWRSSFNPQDPVQASQFKSD 300

Query: 331 GKILYCLELAKYFHHNEDNIIDQEVTHLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKL 390
           GKILYCLELAKYFHH E NIIDQEV  LLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKL
Sbjct: 301 GKILYCLELAKYFHHTEGNIIDQEVMRLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKL 360

Query: 391 QSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVVI 450
           QSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSV+I
Sbjct: 361 QSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVII 420

Query: 451 PEEEIFYLVAFLTSAVPSSKGNDSLEYILTQNMRILEFCKTANLGAKQYLPHYTTRNEWQ 510
           PEEEIFYLVAFLTSAVPSS+GNDSLEYILTQNMRILEFCKTANLG KQYLPHYTTR EWQ
Sbjct: 421 PEEEIFYLVAFLTSAVPSSRGNDSLEYILTQNMRILEFCKTANLGVKQYLPHYTTRKEWQ 480

Query: 511 AHFGPMWETYVQRKTAYDPLAILAPGQRIFQKAKSLS 548
           AHFGPMWETYVQRK AYDPLAILAPGQRIFQKAKSLS
Sbjct: 481 AHFGPMWETYVQRKAAYDPLAILAPGQRIFQKAKSLS 517

BLAST of Lsi04G008060 vs. NCBI nr
Match: XP_038892565.1 (cytokinin dehydrogenase 6-like [Benincasa hispida])

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 532/547 (97.26%), Postives = 539/547 (98.54%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLPLLSSLTSETCTQRKIR PPVSFLRQNNMLSIRSF+LFFL+FIAVRMNLCFTGIPSSL
Sbjct: 1   MLPLLSSLTSETCTQRKIRYPPVSFLRQNNMLSIRSFILFFLSFIAVRMNLCFTGIPSSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA
Sbjct: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGL 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVH+RNFTYIDVSGGELWINILHESLK+GL
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHSRNFTYIDVSGGELWINILHESLKYGL 180

Query: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240
           TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF
Sbjct: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240

Query: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGF 300
           YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDF TFSREQELLISA+ TFDYIEGF
Sbjct: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFTTFSREQELLISAKNTFDYIEGF 300

Query: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLS 360
           VIINRTGLLNNWRSSFNPQDPVQASQF SDGKILYCLELAKYFHH EDNIIDQEVT LLS
Sbjct: 301 VIINRTGLLNNWRSSFNPQDPVQASQFNSDGKILYCLELAKYFHHTEDNIIDQEVTRLLS 360

Query: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420
           QLSYIPSTLFISEVTYV+FLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF
Sbjct: 361 QLSYIPSTLFISEVTYVDFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420

Query: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILT 480
           GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSS+GNDSLEYILT
Sbjct: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSRGNDSLEYILT 480

Query: 481 QNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540
           QN RILEFCKTANLG KQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF
Sbjct: 481 QNTRILEFCKTANLGVKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540

Query: 541 QKAKSLS 548
           QKAKSLS
Sbjct: 541 QKAKSLS 547

BLAST of Lsi04G008060 vs. NCBI nr
Match: XP_008445865.1 (PREDICTED: cytokinin dehydrogenase 6 [Cucumis melo])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.3e-309
Identity = 523/547 (95.61%), Postives = 535/547 (97.81%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLPLLSSLTSETCTQRK R PPVSFLRQNNMLSIRSF++FFL+FIA RMNLCFTGIPSSL
Sbjct: 1   MLPLLSSLTSETCTQRKTRYPPVSFLRQNNMLSIRSFVIFFLSFIAFRMNLCFTGIPSSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           KMLSI+GHFEFD+VQFAARDFGNRYQF+PAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA
Sbjct: 61  KMLSIDGHFEFDQVQFAARDFGNRYQFFPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGL 180
           ARGHGHSLHGQAQAHQGVVINM+SLQGPQMQVHTRNFTYIDVSGGELWINILHESLK+GL
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMKSLQGPQMQVHTRNFTYIDVSGGELWINILHESLKYGL 180

Query: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240
           TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGDVVNCSKEQNSDLF
Sbjct: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGDVVNCSKEQNSDLF 240

Query: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGF 300
           YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDF TFSREQELLISA+ TFDYIEGF
Sbjct: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFTTFSREQELLISAQNTFDYIEGF 300

Query: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLS 360
           VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHH E NIIDQEV  LLS
Sbjct: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHTEGNIIDQEVMRLLS 360

Query: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420
           QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF
Sbjct: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420

Query: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILT 480
           GNILKETSNGPVLIYPVNKSKWDNRTSV+IPEEEIFYLVAFLTSAVPSS+GNDSLEYILT
Sbjct: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVIIPEEEIFYLVAFLTSAVPSSRGNDSLEYILT 480

Query: 481 QNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540
           QNMRILEFCKTANLG KQYLPHYTTR EWQAHFGPMWETYVQRK AYDPLAILAPGQRIF
Sbjct: 481 QNMRILEFCKTANLGVKQYLPHYTTRKEWQAHFGPMWETYVQRKAAYDPLAILAPGQRIF 540

Query: 541 QKAKSLS 548
           QKAKSLS
Sbjct: 541 QKAKSLS 547

BLAST of Lsi04G008060 vs. NCBI nr
Match: XP_004143695.1 (cytokinin dehydrogenase 6 [Cucumis sativus] >KAE8647962.1 hypothetical protein Csa_000209 [Cucumis sativus])

HSP 1 Score: 1063.1 bits (2748), Expect = 7.8e-307
Identity = 520/547 (95.06%), Postives = 534/547 (97.62%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLPLLSSLTSETCTQRK R  PVSFLRQNNMLS+RSF+LFFL+FIA RMNLCFTGIPSSL
Sbjct: 1   MLPLLSSLTSETCTQRKTRYAPVSFLRQNNMLSLRSFVLFFLSFIAFRMNLCFTGIPSSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           KMLSI+GHFEFD+VQ AARDFGNRYQF+PAAILHPSSVSDIAMT+KHIWKMGPRSQLTVA
Sbjct: 61  KMLSIDGHFEFDQVQVAARDFGNRYQFFPAAILHPSSVSDIAMTLKHIWKMGPRSQLTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGL 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLK+GL
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKYGL 180

Query: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240
           TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGDVVNCSKEQNSDLF
Sbjct: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGDVVNCSKEQNSDLF 240

Query: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGF 300
           YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDF TFSREQELLISA+ TFDYIEGF
Sbjct: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFTTFSREQELLISAQNTFDYIEGF 300

Query: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLS 360
           VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHH E NII+QEVT LLS
Sbjct: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHTEGNIINQEVTRLLS 360

Query: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420
           QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF
Sbjct: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420

Query: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILT 480
           GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSS+GNDSLEYILT
Sbjct: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSRGNDSLEYILT 480

Query: 481 QNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540
           QNMRILEFC+TANLG KQYLPHYTTR EWQAHFGPMWETY+QRK AYDPLAILAPGQRIF
Sbjct: 481 QNMRILEFCQTANLGVKQYLPHYTTRKEWQAHFGPMWETYLQRKAAYDPLAILAPGQRIF 540

Query: 541 QKAKSLS 548
           QKAKSLS
Sbjct: 541 QKAKSLS 547

BLAST of Lsi04G008060 vs. NCBI nr
Match: XP_022139273.1 (cytokinin dehydrogenase 6 [Momordica charantia])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.4e-303
Identity = 511/547 (93.42%), Postives = 532/547 (97.26%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLPLLSSLTSETCTQRK+R PPVSFLRQNNMLSIRSFM+ FL+ IA+RMNLCF GIPSSL
Sbjct: 1   MLPLLSSLTSETCTQRKLRYPPVSFLRQNNMLSIRSFMILFLSCIALRMNLCFNGIPSSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           KMLSINGHFEFDKVQ+AARDFGNRYQ+YPAAILHPSSVSDIAMTIKHIWKMGPRS+LTVA
Sbjct: 61  KMLSINGHFEFDKVQYAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPRSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGL 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTR+FTYIDV GGELWINILHESLK+GL
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVHTRDFTYIDVPGGELWINILHESLKYGL 180

Query: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240
           TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF
Sbjct: 181 TPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLF 240

Query: 241 YSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGF 300
           YSVLGGLGQFG+ITRAKILLEPAP  VKWIRVLYLDF TFSREQELLISAE TF+YIEGF
Sbjct: 241 YSVLGGLGQFGVITRAKILLEPAPAKVKWIRVLYLDFTTFSREQELLISAENTFNYIEGF 300

Query: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLS 360
           VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYF+HNE NI+DQEV  LLS
Sbjct: 301 VIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFNHNEGNIVDQEVKSLLS 360

Query: 361 QLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVF 420
           +LSYIPSTLFISEVTYVEFLDRV VSAVKLQSKGLWEVPHPWLNLL+PKSKI+KF+EGVF
Sbjct: 361 RLSYIPSTLFISEVTYVEFLDRVHVSAVKLQSKGLWEVPHPWLNLLVPKSKIEKFSEGVF 420

Query: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILT 480
           GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEE+IFYL+AFLTSAVPSSKGND LEYILT
Sbjct: 421 GNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEDIFYLIAFLTSAVPSSKGNDGLEYILT 480

Query: 481 QNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540
           QNMRILEFCKTANLGAKQYLPHYTT+NEW+AHFGPMWETYVQRKTAYDPLAILAPGQRIF
Sbjct: 481 QNMRILEFCKTANLGAKQYLPHYTTKNEWKAHFGPMWETYVQRKTAYDPLAILAPGQRIF 540

Query: 541 QKAKSLS 548
           QKAK LS
Sbjct: 541 QKAKPLS 547

BLAST of Lsi04G008060 vs. NCBI nr
Match: XP_023525504.1 (cytokinin dehydrogenase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.4e-295
Identity = 503/549 (91.62%), Postives = 527/549 (95.99%), Query Frame = 0

Query: 1   MLPLLSSLTSETCTQRKIRNPPVSFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSL 60
           MLP+LSSLTSETC+QRK+R PPVSFL+Q NMLSIRSFM+ FL+ IAVRMNLCFTGIP SL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLSIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  KMLSINGHFEFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120
           +ML INGHFEFDKV+FAARDFGNRYQ+YPAAILHPSSVSDIAMTIKHIWKMGP S+LTVA
Sbjct: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQ-VHTRNFTYIDVSGGELWINILHESLKHG 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQ VHT++FTYIDVSGGELWINIL ESLK+G
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTKSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLY DF TF+REQELLIS E TFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISEENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNED-NIIDQEVTHL 360
           FVIINRTGLLNNWRSSF+PQDPVQASQFKS+GKILYCLELAKYFHHN+D N +DQEV  L
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360

Query: 361 LSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEG 420
           LS+LSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIK+FAEG
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKEFAEG 420

Query: 421 VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYI 480
           VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEE+IFYLVAFLTSAVP S+GNDSLEYI
Sbjct: 421 VFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEDIFYLVAFLTSAVPCSRGNDSLEYI 480

Query: 481 LTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQR 540
           LTQN RILEFCKTANLGAKQYLPHYTTR EWQAHFGPMWETYV+RK AYDPLAILAPGQR
Sbjct: 481 LTQNTRILEFCKTANLGAKQYLPHYTTRKEWQAHFGPMWETYVERKMAYDPLAILAPGQR 540

Query: 541 IFQKAKSLS 548
           IF+KAK LS
Sbjct: 541 IFKKAKPLS 549

BLAST of Lsi04G008060 vs. TAIR 10
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6 )

HSP 1 Score: 750.0 bits (1935), Expect = 1.4e-216
Identity = 363/527 (68.88%), Postives = 427/527 (81.02%), Query Frame = 0

Query: 24  SFLRQNNMLSIRSFMLFFLTFIAVRMNLCFTGIPSSLKMLSINGHFEFDKVQFAARDFGN 83
           S LR+  ML +RSF +  L+ IA ++  CF+   SSLK L + GH EF+ V  A++DFGN
Sbjct: 7   SLLRKRTMLIVRSFTILLLSCIAFKLACCFSSSISSLKALPLVGHLEFEHVHHASKDFGN 66

Query: 84  RYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLHGQAQAHQGVVINME 143
           RYQ  P A+LHP SVSDIA TI+HIW MG  SQLTVAARG GHSL GQAQ   G+VI+ME
Sbjct: 67  RYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHSLQGQAQTRHGIVIHME 126

Query: 144 SLQGPQMQVHTRN--FTYIDVSGGELWINILHESLKHGLTPKSWTDYLHLTVGGTLSNAG 203
           SL   ++QV++ +    Y+DVSGGELWINILHE+LK+GL PKSWTDYLHLTVGGTLSNAG
Sbjct: 127 SLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSWTDYLHLTVGGTLSNAG 186

Query: 204 ISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLFYSVLGGLGQFGIITRAKILLE 263
           ISGQAFRHGPQISNVHQLE+VTGKG+++NC+K QNSDLF  VLGGLGQFGIITRA+I LE
Sbjct: 187 ISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLGGLGQFGIITRARIALE 246

Query: 264 PAPTMVKWIRVLYLDFATFSREQELLISAE-KTFDYIEGFVIINRTGLLNNWRSSFNPQD 323
           PAPTMVKWIRVLYLDFA F+++QE LISA+   FDYIEGFVIINRTGLLN+WR SF  ++
Sbjct: 247 PAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIINRTGLLNSWRLSFTAEE 306

Query: 324 PVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLSQLSYIPSTLFISEVTYVEFL 383
           P++ASQFK DG+ LYCLELAKY   +  ++I+QEV   LS+LSY+ STLF +EV Y  FL
Sbjct: 307 PLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELSYVTSTLFTTEVAYEAFL 366

Query: 384 DRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSNGPVLIYPVNKS 443
           DRV VS VKL+SKG WEVPHPWLNLL+P+SKI +FA GVFGNIL +TSNGPV++YPVNKS
Sbjct: 367 DRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNILTDTSNGPVIVYPVNKS 426

Query: 444 KWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILTQNMRILEFCKTANLGAKQYL 503
           KWDN+TS V PEEE+FYLVA LTSA P S G D +E IL +N RILEF + A +G KQYL
Sbjct: 427 KWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNRRILEFSEEAGIGLKQYL 486

Query: 504 PHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIFQKAKSLS 548
           PHYTTR EW++HFG  W  +V+RK+ YDPLAILAPG RIFQKA S S
Sbjct: 487 PHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKAVSYS 533

BLAST of Lsi04G008060 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 723.8 bits (1867), Expect = 1.0e-208
Identity = 352/533 (66.04%), Postives = 415/533 (77.86%), Query Frame = 0

Query: 25  FLRQNNMLSIRSFMLFFLTFIAVRMNLCF---------------TGIPSSLKMLSINGHF 84
           F RQNN   +  FM+  L+ I  R NLC                + I SSL  L + G+ 
Sbjct: 9   FHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRSSLVSLDLEGYI 68

Query: 85  EFDKVQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVAARGHGHSLH 144
            FD V   A+DFGNRYQ  P AILHP SV DI+  +KHI  +G  S LTVAARGHGHSL 
Sbjct: 69  SFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSLQ 128

Query: 145 GQAQAHQGVVINMESLQGPQMQVHTRNFTYIDVSGGELWINILHESLKHGLTPKSWTDYL 204
           GQA AHQGVVI MESL+ P ++++     Y+DVSGGE+WINIL E+LK+GL+PKSWTDYL
Sbjct: 129 GQALAHQGVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWTDYL 188

Query: 205 HLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLFYSVLGGLGQ 264
           HLTVGGTLSNAGISGQAF+HGPQI+NV+QLE+VTGKG+VV CS+++NS+LF+SVLGGLGQ
Sbjct: 189 HLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLGQ 248

Query: 265 FGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGFVIINRTGLL 324
           FGIITRA+I LEPAP MVKWIRVLY DF+ FSR+QE LIS EKTFDY+EGFVIINRT LL
Sbjct: 249 FGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDLL 308

Query: 325 NNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLSQLSYIPSTL 384
           NNWRSSF+P D  QAS+FKSDGK LYCLE+ KYF+  E + +DQE   LLS+L+YIPSTL
Sbjct: 309 NNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPSTL 368

Query: 385 FISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNILKETSN 444
           F SEV Y+EFLDRV ++  KL++KGLWEVPHPWLNLLIPKS I +FA  VF NIL   +N
Sbjct: 369 FSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNNN 428

Query: 445 GPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILTQNMRILEFC 504
           GP+LIYPVN+SKW   TS++ P E+IFYLVAFL SAVP+S G + LEY+L QN R++ FC
Sbjct: 429 GPILIYPVNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNFC 488

Query: 505 KTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIFQK 543
             ANL  KQYLPHY T+ EW++HFG  WET+ QRK AYDPLAILAPGQRIFQK
Sbjct: 489 AAANLNVKQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQK 541

BLAST of Lsi04G008060 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 540.4 bits (1391), Expect = 1.6e-153
Identity = 263/487 (54.00%), Postives = 351/487 (72.07%), Query Frame = 0

Query: 63  LSINGHFEFDKVQFA--ARDFGN-RYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTV 122
           + ++GHF       A  + DFG  +    P A+LHPSS  D+A  ++  +  G  +   V
Sbjct: 38  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 97

Query: 123 AARGHGHSLHGQAQA-HQGVVINM-ESLQGPQMQVHTRNFTYIDVSGGELWINILHESLK 182
           +ARGHGHS++GQA A   GVV+ M   + G    +   +  Y+DV GGELW+++L ++L+
Sbjct: 98  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEMYVDVWGGELWVDVLKKTLE 157

Query: 183 HGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNS 242
           HGL PKSWTDYL+LTVGGTLSNAGISGQAF HGPQISNV +L+VVTGKG+V+ CS+E+N+
Sbjct: 158 HGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENT 217

Query: 243 DLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLIS--AEKTFD 302
            LF+ VLGGLGQFGIITRA+I LEPAP  V+WIRVLY  F  F+ +QE LIS   +  FD
Sbjct: 218 RLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFD 277

Query: 303 YIEGFVIINRTGLLNNWRSS-FNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQE 362
           Y+EGFVI++  GL+NNWRSS F+P++PV+ S   S+G +LYCLE+ K +H ++  I+DQE
Sbjct: 278 YVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQE 337

Query: 363 VTHLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKK 422
           V  L+ +L++IP+++F +++ YV+FLDRV  + +KL+SK LWEVPHPWLNL +PKS+I  
Sbjct: 338 VEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISD 397

Query: 423 FAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDS 482
           F +GVF  IL   ++GP+LIYP+NK KWD R+S V P+EE+FYLVA L SA+   +    
Sbjct: 398 FDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQK 457

Query: 483 LEYILTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILA 542
           LEY+  QN RILEFC+ A +  KQYLPH+ T+ EW AHFG  W+ +   K  +DP  ILA
Sbjct: 458 LEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILA 517

BLAST of Lsi04G008060 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 535.8 bits (1379), Expect = 4.0e-152
Identity = 263/488 (53.89%), Postives = 351/488 (71.93%), Query Frame = 0

Query: 63  LSINGHFEFDKVQFA--ARDFGN-RYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLTV 122
           + ++GHF       A  + DFG  +    P A+LHPSS  D+A  ++  +  G  +   V
Sbjct: 34  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 93

Query: 123 AARGHGHSLHGQAQA-HQGVVINM-ESLQGPQMQVHTRNFTYIDVSGGELWINILHESLK 182
           +ARGHGHS++GQA A   GVV+ M   + G    +   +  Y+DV GGELW+++L ++L+
Sbjct: 94  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEMYVDVWGGELWVDVLKKTLE 153

Query: 183 HGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVT-GKGDVVNCSKEQN 242
           HGL PKSWTDYL+LTVGGTLSNAGISGQAF HGPQISNV +L+VVT GKG+V+ CS+E+N
Sbjct: 154 HGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTVGKGEVMRCSEEEN 213

Query: 243 SDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLIS--AEKTF 302
           + LF+ VLGGLGQFGIITRA+I LEPAP  V+WIRVLY  F  F+ +QE LIS   +  F
Sbjct: 214 TRLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKF 273

Query: 303 DYIEGFVIINRTGLLNNWRSS-FNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQ 362
           DY+EGFVI++  GL+NNWRSS F+P++PV+ S   S+G +LYCLE+ K +H ++  I+DQ
Sbjct: 274 DYVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQ 333

Query: 363 EVTHLLSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIK 422
           EV  L+ +L++IP+++F +++ YV+FLDRV  + +KL+SK LWEVPHPWLNL +PKS+I 
Sbjct: 334 EVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRIS 393

Query: 423 KFAEGVFGNILKETSNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGND 482
            F +GVF  IL   ++GP+LIYP+NK KWD R+S V P+EE+FYLVA L SA+   +   
Sbjct: 394 DFDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQ 453

Query: 483 SLEYILTQNMRILEFCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAIL 542
            LEY+  QN RILEFC+ A +  KQYLPH+ T+ EW AHFG  W+ +   K  +DP  IL
Sbjct: 454 KLEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHIL 513

BLAST of Lsi04G008060 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 479.2 bits (1232), Expect = 4.5e-135
Identity = 232/478 (48.54%), Postives = 332/478 (69.46%), Query Frame = 0

Query: 74  VQFAARDFGNRYQFYPAAILHPSSVSDIAMTIKHIWKMGPRSQLT--VAARGHGHSLHGQ 133
           V+ AA DFG+  + +P+A+L PSSV DI   I    K+   SQL+  +AARGHGHS  GQ
Sbjct: 55  VESAATDFGHVTKIFPSAVLIPSSVEDITDLI----KLSFDSQLSFPLAARGHGHSHRGQ 114

Query: 134 AQAHQGVVINMESLQGPQMQVH-TRNFTYIDVSGGELWINILHESLKHGLTPKSWTDYLH 193
           A A  GVV+NM S+      +  +R   Y+DV    LWI +L+++L+ GLTP SWTDYL+
Sbjct: 115 ASAKDGVVVNMRSMVNRDRGIKVSRTCLYVDVDAAWLWIEVLNKTLELGLTPVSWTDYLY 174

Query: 194 LTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDLFYSVLGGLGQF 253
           LTVGGTLSN GISGQ FR+GPQI+NV +++V+TGKG++  CSK+ NSDLF++VLGGLGQF
Sbjct: 175 LTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMNSDLFFAVLGGLGQF 234

Query: 254 GIITRAKILLEPAPTMVKWIRVLYLDFATFSREQELLISAEKTFDYIEGFVIINRTGLLN 313
           GIITRA+I LE AP   KW+R LY+DF+ F+R+QE +IS     D++EG ++++  G  +
Sbjct: 235 GIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQERVISKTDGVDFLEGSIMVDH-GPPD 294

Query: 314 NWRSS-FNPQDPVQASQFKSDGKILYCLELAKYFHHNEDNIIDQEVTHLLSQLSYIPSTL 373
           NWRS+ + P D ++ +      +++YCLE+ KY+       +++E+  L   L+++   +
Sbjct: 295 NWRSTYYPPSDHLRIASMVKRHRVIYCLEVVKYYDETSQYTVNEEMEELSDSLNHVRGFM 354

Query: 374 FISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWLNLLIPKSKIKKFAEGVFGNIL--KET 433
           +  +VTY++FL+RV+   + L+SKG W+VPHPWLNL +PK++I KF +GVF  I+     
Sbjct: 355 YEKDVTYMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDDGVFKGIILRNNI 414

Query: 434 SNGPVLIYPVNKSKWDNRTSVVIPEEEIFYLVAFLTSAVPSSKGNDSLEYILTQNMRILE 493
           ++GPVL+YP+N++KW++R S  IPEE++FY V FL SA     G D+ E    +NM IL+
Sbjct: 415 TSGPVLVYPMNRNKWNDRMSAAIPEEDVFYAVGFLRSA-----GFDNWEAFDQENMEILK 474

Query: 494 FCKTANLGAKQYLPHYTTRNEWQAHFGPMWETYVQRKTAYDPLAILAPGQRIFQKAKS 546
           FC+ AN+G  QYLP+++++  W  HFGP W  +V+RK  YDP  IL+PGQ IFQK  S
Sbjct: 475 FCEDANMGVIQYLPYHSSQEGWVRHFGPRWNIFVERKYKYDPKMILSPGQNIFQKINS 522

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LY711.9e-21568.88Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2[more]
O222131.5e-20766.04Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1[more]
Q75K786.2e-20667.55Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 ... [more]
Q5JLP44.3e-19966.40Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 ... [more]
Q67YU02.3e-15254.00Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BD713.1e-30995.61Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488756 PE=3 SV=1[more]
A0A6J1CFB16.7e-30493.42Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111010228 PE=3 SV=1[more]
A0A6J1FSN02.2e-29491.26Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111446933 PE=3 SV=1[more]
A0A6J1JHC81.1e-29390.89Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111485722 PE=3 SV=1[more]
A0A5A7SXR43.1e-29395.74Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
XP_038892565.10.0e+0097.26cytokinin dehydrogenase 6-like [Benincasa hispida][more]
XP_008445865.16.3e-30995.61PREDICTED: cytokinin dehydrogenase 6 [Cucumis melo][more]
XP_004143695.17.8e-30795.06cytokinin dehydrogenase 6 [Cucumis sativus] >KAE8647962.1 hypothetical protein C... [more]
XP_022139273.11.4e-30393.42cytokinin dehydrogenase 6 [Momordica charantia][more]
XP_023525504.12.4e-29591.62cytokinin dehydrogenase 6-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G63440.11.4e-21668.88cytokinin oxidase/dehydrogenase 6 [more]
AT2G41510.11.0e-20866.04cytokinin oxidase/dehydrogenase 1 [more]
AT1G75450.11.6e-15354.00cytokinin oxidase 5 [more]
AT1G75450.24.0e-15253.89cytokinin oxidase 5 [more]
AT5G56970.14.5e-13548.54cytokinin oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 142..265
e-value: 8.0E-218
score: 725.6
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 89..232
e-value: 5.9E-19
score: 68.2
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 264..540
e-value: 3.1E-116
score: 387.7
IPR016170Cytokinin dehydrogenase, C-terminal domain superfamilyGENE3D3.40.462.10coord: 266..499
e-value: 8.0E-218
score: 725.6
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 74..540
e-value: 8.0E-218
score: 725.6
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 30..544
NoneNo IPR availablePANTHERPTHR13878:SF53CYTOKININ DEHYDROGENASE 6coord: 30..544
IPR006093Oxygen oxidoreductase covalent FAD-binding sitePROSITEPS00862OX2_COVAL_FADcoord: 89..124
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 85..263
score: 14.324731
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 71..262
IPR016164FAD-linked oxidase-like, C-terminalSUPERFAMILY55103FAD-linked oxidases, C-terminal domaincoord: 264..540

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G008060.1Lsi04G008060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009690 cytokinin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity