Lsi04G007860 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G007860
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	GRAM domain-containing protein
Location	chr04: 7394056 .. 7395103 (+)
RNA-Seq Expression	Lsi04G007860
Synteny	Lsi04G007860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCAAGTTCAATGATCAGAGAGCTTGGAAGAAAAGGTTACAGATTTGTTAATGGAATCCTTGAACATGGTAAACATGATTTGCTTTTTTCTTCATTTGAGAAAGAATCTCTCTCTGAATTTTACTTTCTTTCCTAGCTGATTATAATGATGAAATGATTGATTGGATTGGATTGTCCTCTTTTGTTTATCTCCCTAGAAATGCTAGAAACTTCCATTTCTCGATAAATGCAATGCATATACGAATAATGAATTGTTGTAGGTCGATATGAACATGATTCAACTAGTTAAAGCAAATATTACTACATGATTACAAAGTCTGTCCTACAGAGCGTTAAAAACCAAAAACGAAAGCATTACTCGTCTCTCTTTCTATGTAAACAGAGATTGACATTGATTCTTCCTGCATTGATAATAATGTATATATTAAAAATATATACTCGATTCGAAGGATTAAAAATGATTGACAGTTACATTGGAATGACGACGTGCAGTTAAACTGTCGCCGAACATCGCCAAAACCGTTAAGGGGAAGCTGTGGTTGGGGACAAAACTTGTCCAGTTTGGAGGAAGTGAGAAGATTTTTCAGAAGATGTTCAATCTCAGACAAGGAGACAAGCTGCTGAGTTCTGCACAATGTTATTTGTCAACCACAGCAGGTCCCATAGCAGGTCTCCTCTTTGTCTCCACCCATGTAGTTGCCTTCTGCAGTGACAGACCAATCATAATTTCTTCTCCACAAGGAGAACTAGGCAGATTGTTTTACAAAGTACATAATAATTCCTTTAACATTTACATTTGACTGTTTTTCCTCTTTCTATCTCTCTCTCTCTCTGCCTGGTTGCTAAGTTTTTTCTACAATGCTTATTTCCTCAGGTTATGATCCCAGTGAAGAAGGTGAAGAGAGTTAACCAAAGAAACAATGTAAGCAATCCATCAAAAAAGTACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATTATGAGAAAGCAGTTAAGTTCCTCCAAAAGGGGGTTTTTTCTAAGGAT

mRNA sequence

CTCAAGTTCAATGATCAGAGAGCTTGGAAGAAAAGGTTACAGATTTGTTAATGGAATCCTTGAACATGCTGATTATAATGATGAAATGATTGATTGGATTGGATTGTCCTCTTTTGTTTATCTCCCTAGAAATGCTAGAAACTTCCATTTCTCGATAAATGCAATGCATATACGAATAATGAATTGTTGTAGCAAATATTACTACATGATTACAAAGTCTGTCCTACAGAGCGTTAAAAACCAAAAACGAAAGCATTACTCGTCTCTCTTTCTATGTAAACAGAGATTGACATTGATTCTTCCTGCATTGATAATAATGTATATATTAAAAATATATACTCGATTCGAAGGATTAAAAATGATTGACATTAAACTGTCGCCGAACATCGCCAAAACCGTTAAGGGGAAGCTGTGGTTGGGGACAAAACTTGTCCAGTTTGGAGGAAGTGAGAAGATTTTTCAGAAGATGTTCAATCTCAGACAAGGAGACAAGCTGCTGAGTTCTGCACAATGTTATTTGTCAACCACAGCAGGTCCCATAGCAGGTCTCCTCTTTGTCTCCACCCATGTAGTTGCCTTCTGCAGTGACAGACCAATCATAATTTCTTCTCCACAAGGAGAACTAGGCAGATTGTTTTACAAAGTTATGATCCCAGTGAAGAAGGTGAAGAGAGTTAACCAAAGAAACAATGTAAGCAATCCATCAAAAAAGTACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATTATGAGAAAGCAGTTAAGTTCCTCCAAAAGGGGGTTTTTTCTAAGGAT

Coding sequence (CDS)

ATGATCAGAGAGCTTGGAAGAAAAGGTTACAGATTTGTTAATGGAATCCTTGAACATGCTGATTATAATGATGAAATGATTGATTGGATTGGATTGTCCTCTTTTGTTTATCTCCCTAGAAATGCTAGAAACTTCCATTTCTCGATAAATGCAATGCATATACGAATAATGAATTGTTGTAGCAAATATTACTACATGATTACAAAGTCTGTCCTACAGAGCGTTAAAAACCAAAAACGAAAGCATTACTCGTCTCTCTTTCTATGTAAACAGAGATTGACATTGATTCTTCCTGCATTGATAATAATGTATATATTAAAAATATATACTCGATTCGAAGGATTAAAAATGATTGACATTAAACTGTCGCCGAACATCGCCAAAACCGTTAAGGGGAAGCTGTGGTTGGGGACAAAACTTGTCCAGTTTGGAGGAAGTGAGAAGATTTTTCAGAAGATGTTCAATCTCAGACAAGGAGACAAGCTGCTGAGTTCTGCACAATGTTATTTGTCAACCACAGCAGGTCCCATAGCAGGTCTCCTCTTTGTCTCCACCCATGTAGTTGCCTTCTGCAGTGACAGACCAATCATAATTTCTTCTCCACAAGGAGAACTAGGCAGATTGTTTTACAAAGTTATGATCCCAGTGAAGAAGGTGAAGAGAGTTAACCAAAGAAACAATGTAAGCAATCCATCAAAAAAGTACATTCAAGTGGTGACTGTGGATGATTTTGACTTCTGGTTTATGGGTTTTTTGAATTATGAGAAAGCAGTTAAGTTCCTCCAAAAGGGGGTTTTTTCTAAGGAT

Protein sequence

MIRELGRKGYRFVNGILEHADYNDEMIDWIGLSSFVYLPRNARNFHFSINAMHIRIMNCCSKYYYMITKSVLQSVKNQKRKHYSSLFLCKQRLTLILPALIIMYILKIYTRFEGLKMIDIKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGLLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVTVDDFDFWFMGFLNYEKAVKFLQKGVFSKD

Homology

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BLAST of Lsi04G007860 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.5e-43
Identity = 82/145 (56.55%), Postives = 111/145 (76.55%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           K+ P + +TVK KL LG +++Q GG EKIF+++F + +G+KL    QCYLSTTAGPIAGL
Sbjct: 68  KIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGL 127

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I + SPQG++ R+ YKV IP+ K+ RVNQ  N   PS+KY++VVT
Sbjct: 128 LFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVT 187

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           VD FDFWFMGFL+Y+KA   L+K +
Sbjct: 188 VDGFDFWFMGFLSYQKAFNCLEKAL 212

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BLAST of Lsi04G007860 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.3e-43
Identity = 81/145 (55.86%), Postives = 111/145 (76.55%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           KL P + +TVK KL LG +++Q GG EKI++++F +   +KL  + QCYLSTTAGPIAGL
Sbjct: 69  KLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGL 128

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I ++SPQGEL R+ YKV IP+ K+  VNQ  N + PS+KY++VVT
Sbjct: 129 LFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYLEVVT 188

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           VD FDFWFMGFL+Y+KA   L++ +
Sbjct: 189 VDGFDFWFMGFLSYQKAFNCLEQAL 213

[Close]

BLAST of Lsi04G007860 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 174.1 bits (440), Expect = 2.1e-42
Identity = 79/145 (54.48%), Postives = 110/145 (75.86%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           KL P + +TVK KL LG K++Q GG EKI++++F +   +KL  + QCYLSTTAGPIAGL
Sbjct: 69  KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGL 128

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I ++SPQG L R+ YKV IP+ K+  VNQ  N   PS+KY+++VT
Sbjct: 129 LFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKKPSQKYLEIVT 188

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           +D+FDFWFMGF++Y+KA   L+K +
Sbjct: 189 IDNFDFWFMGFVSYQKAFNCLEKAL 213

[Close]

BLAST of Lsi04G007860 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.2e-40
Identity = 77/145 (53.10%), Postives = 109/145 (75.17%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           KL P + +TVK KL LG K++Q GG EKI++++F +   +KL  + QCYLSTT G IAGL
Sbjct: 61  KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYLSTTEGSIAGL 120

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I ++SPQG+L R+ YKV IP+ K+  VNQ  N   PS++Y++VVT
Sbjct: 121 LFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKKPSQRYLEVVT 180

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           VD++DFWFMGF++Y+KA   L+K +
Sbjct: 181 VDNYDFWFMGFVSYQKAFNCLEKAL 205

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BLAST of Lsi04G007860 vs. ExPASy Swiss-Prot
Match: Q9SE96 (GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 9.3e-30
Identity = 63/148 (42.57%), Postives = 98/148 (66.22%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +K  P++A     ++  GTK++  GG EK+F++ F+    +KLL +  CYLST+AGP+ G
Sbjct: 110 LKTGPSVADAAVSRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKTYACYLSTSAGPVLG 169

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           ++++STH +AF SD P+     +  L   +YKV++P  ++K VN   +  N S KYIQV+
Sbjct: 170 VMYLSTHKLAFSSDNPLSYKEGEQTLWS-YYKVVLPANQLKAVNPSTSRVNTSDKYIQVI 229

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGVFS 268
           ++D+ +FWFMGF+ YE AVK LQ+ V S
Sbjct: 230 SIDNHEFWFMGFVTYESAVKSLQEAVQS 256

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BLAST of Lsi04G007860 vs. ExPASy TrEMBL
Match: A0A5A7SSY1 (GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00680 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.4e-68
Identity = 129/146 (88.36%), Postives = 138/146 (94.52%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPNIAKTVKGKLWLGTKLVQFGGSEKIF KMFNL QGDKLL+SA CYLSTTAGPIAG
Sbjct: 6   VKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 65

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           L+FVSTHVVAFCSDRPIIISSP GE+G++FYKVMIPV KVKRVNQRNN +NP+KKYIQVV
Sbjct: 66  LVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVKRVNQRNNENNPAKKYIQVV 125

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLNYEK  KF+QKGV
Sbjct: 126 TVDDFDFWFMGFLNYEKTFKFIQKGV 151

[Close]

BLAST of Lsi04G007860 vs. ExPASy TrEMBL
Match: A0A1S3BDR8 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488777 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.4e-68
Identity = 129/146 (88.36%), Postives = 138/146 (94.52%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPNIAKTVKGKLWLGTKLVQFGGSEKIF KMFNL QGDKLL+SA CYLSTTAGPIAG
Sbjct: 20  VKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 79

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           L+FVSTHVVAFCSDRPIIISSP GE+G++FYKVMIPV KVKRVNQRNN +NP+KKYIQVV
Sbjct: 80  LVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVKRVNQRNNENNPAKKYIQVV 139

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLNYEK  KF+QKGV
Sbjct: 140 TVDDFDFWFMGFLNYEKTFKFIQKGV 165

[Close]

BLAST of Lsi04G007860 vs. ExPASy TrEMBL
Match: A0A0A0KPM0 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G577990 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 2.0e-67
Identity = 126/146 (86.30%), Postives = 137/146 (93.84%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPNIAKTVKGKLWLGTKL+QFGGSE IF KMFNL  GDKLL+SA CYLSTTAGPIAG
Sbjct: 20  VKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYLSTTAGPIAG 79

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           L+FVSTHVVAFCSDRPIIISSP GE+G++FYKVMIPV KVKRVNQRNNV+NP+KKYIQVV
Sbjct: 80  LIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNPAKKYIQVV 139

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLN+EKA KF+Q GV
Sbjct: 140 TVDDFDFWFMGFLNHEKAFKFIQNGV 165

[Close]

BLAST of Lsi04G007860 vs. ExPASy TrEMBL
Match: A0A6J1CD83 (putative GEM-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111010135 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 1.2e-64
Identity = 125/146 (85.62%), Postives = 134/146 (91.78%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPN+A+TVKGKLWL  KLVQFGGS KIFQK+FNLRQGDKLLSS+ CYLSTTAGPIAG
Sbjct: 23  VKLSPNVAETVKGKLWLVAKLVQFGGSGKIFQKLFNLRQGDKLLSSSHCYLSTTAGPIAG 82

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           LLF+ST+ VAFCSDRPIII+SP GELGR  YKVMIPVKK+KRVNQ  NVSNPSKKYIQVV
Sbjct: 83  LLFISTNAVAFCSDRPIIITSPHGELGRFNYKVMIPVKKMKRVNQTENVSNPSKKYIQVV 142

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLNY KA KFLQKGV
Sbjct: 143 TVDDFDFWFMGFLNYGKAFKFLQKGV 168

[Close]

BLAST of Lsi04G007860 vs. ExPASy TrEMBL
Match: A0A6J1JHW6 (GEM-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111487132 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 3.8e-58
Identity = 116/174 (66.67%), Postives = 136/174 (78.16%), Query Frame = 0


Query: 118 IDIKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPI 177
           +++KLSPNI + +KGKLW G +LVQFGG+EKIFQKMFNL QG+KLLS ++CYLSTTAGP+
Sbjct: 5   VEVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPV 64

Query: 178 AGLLFVSTHVVAFCSDRPIIISSPQGELGRLFYK-------------------------- 237
           AGLLF+STHVVAFCSDRPI++SS +GELGRL YK                          
Sbjct: 65  AGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVTLYILQHSQTPVVANKFFVLCLIHQ 124

Query: 238 VMIPVKKVKRVNQRNNVSNPSKKYIQVVTVDDFDFWFMGFLNYEKAVKFLQKGV 266
           VMIP+KK+KRVNQ  +++NPSKKYIQVVTVDDFDFWFMGFLNYEKA KFLQK V
Sbjct: 125 VMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKV 178

[Close]

BLAST of Lsi04G007860 vs. NCBI nr
Match: XP_038893009.1 (GEM-like protein 4 [Benincasa hispida])

HSP 1 Score: 279.6 bits (714), Expect = 2.8e-71
Identity = 140/150 (93.33%), Postives = 144/150 (96.00%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPNIAKTVKGKLWLGTKLVQF GSEKIF KMFNL+QGDKLLSSA CYLST+AGPIAG
Sbjct: 20  VKLSPNIAKTVKGKLWLGTKLVQF-GSEKIFHKMFNLKQGDKLLSSAHCYLSTSAGPIAG 79

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKV RVNQRNNVSNPSKKYIQVV
Sbjct: 80  LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVGRVNQRNNVSNPSKKYIQVV 139

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGVFSKD 270
           TVD FDFWFMGFLNYEKA KFLQKGVFS+D
Sbjct: 140 TVDGFDFWFMGFLNYEKAFKFLQKGVFSED 168

[Close]

BLAST of Lsi04G007860 vs. NCBI nr
Match: XP_008445893.1 (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 269.6 bits (688), Expect = 2.9e-68
Identity = 129/146 (88.36%), Postives = 138/146 (94.52%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPNIAKTVKGKLWLGTKLVQFGGSEKIF KMFNL QGDKLL+SA CYLSTTAGPIAG
Sbjct: 20  VKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 79

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           L+FVSTHVVAFCSDRPIIISSP GE+G++FYKVMIPV KVKRVNQRNN +NP+KKYIQVV
Sbjct: 80  LVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVKRVNQRNNENNPAKKYIQVV 139

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLNYEK  KF+QKGV
Sbjct: 140 TVDDFDFWFMGFLNYEKTFKFIQKGV 165

[Close]

BLAST of Lsi04G007860 vs. NCBI nr
Match: KAA0034080.1 (GEM-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 269.6 bits (688), Expect = 2.9e-68
Identity = 129/146 (88.36%), Postives = 138/146 (94.52%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPNIAKTVKGKLWLGTKLVQFGGSEKIF KMFNL QGDKLL+SA CYLSTTAGPIAG
Sbjct: 6   VKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGDKLLNSAHCYLSTTAGPIAG 65

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           L+FVSTHVVAFCSDRPIIISSP GE+G++FYKVMIPV KVKRVNQRNN +NP+KKYIQVV
Sbjct: 66  LVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVKRVNQRNNENNPAKKYIQVV 125

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLNYEK  KF+QKGV
Sbjct: 126 TVDDFDFWFMGFLNYEKTFKFIQKGV 151

[Close]

BLAST of Lsi04G007860 vs. NCBI nr
Match: XP_004147134.1 (GEM-like protein 4 [Cucumis sativus] >KGN51560.1 hypothetical protein Csa_008945 [Cucumis sativus])

HSP 1 Score: 265.8 bits (678), Expect = 4.1e-67
Identity = 126/146 (86.30%), Postives = 137/146 (93.84%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPNIAKTVKGKLWLGTKL+QFGGSE IF KMFNL  GDKLL+SA CYLSTTAGPIAG
Sbjct: 20  VKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGDKLLNSAHCYLSTTAGPIAG 79

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           L+FVSTHVVAFCSDRPIIISSP GE+G++FYKVMIPV KVKRVNQRNNV+NP+KKYIQVV
Sbjct: 80  LIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVKRVNQRNNVNNPAKKYIQVV 139

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLN+EKA KF+Q GV
Sbjct: 140 TVDDFDFWFMGFLNHEKAFKFIQNGV 165

[Close]

BLAST of Lsi04G007860 vs. NCBI nr
Match: XP_022139162.1 (putative GEM-like protein 8 [Momordica charantia])

HSP 1 Score: 256.5 bits (654), Expect = 2.5e-64
Identity = 125/146 (85.62%), Postives = 134/146 (91.78%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +KLSPN+A+TVKGKLWL  KLVQFGGS KIFQK+FNLRQGDKLLSS+ CYLSTTAGPIAG
Sbjct: 23  VKLSPNVAETVKGKLWLVAKLVQFGGSGKIFQKLFNLRQGDKLLSSSHCYLSTTAGPIAG 82

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           LLF+ST+ VAFCSDRPIII+SP GELGR  YKVMIPVKK+KRVNQ  NVSNPSKKYIQVV
Sbjct: 83  LLFISTNAVAFCSDRPIIITSPHGELGRFNYKVMIPVKKMKRVNQTENVSNPSKKYIQVV 142

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGV 266
           TVDDFDFWFMGFLNY KA KFLQKGV
Sbjct: 143 TVDDFDFWFMGFLNYGKAFKFLQKGV 168

[Close]

BLAST of Lsi04G007860 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 177.9 bits (450), Expect = 1.1e-44
Identity = 82/145 (56.55%), Postives = 111/145 (76.55%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           K+ P + +TVK KL LG +++Q GG EKIF+++F + +G+KL    QCYLSTTAGPIAGL
Sbjct: 68  KIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGL 127

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I + SPQG++ R+ YKV IP+ K+ RVNQ  N   PS+KY++VVT
Sbjct: 128 LFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVT 187

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           VD FDFWFMGFL+Y+KA   L+K +
Sbjct: 188 VDGFDFWFMGFLSYQKAFNCLEKAL 212

[Close]

BLAST of Lsi04G007860 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 176.4 bits (446), Expect = 3.1e-44
Identity = 81/145 (55.86%), Postives = 111/145 (76.55%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           KL P + +TVK KL LG +++Q GG EKI++++F +   +KL  + QCYLSTTAGPIAGL
Sbjct: 69  KLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGL 128

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I ++SPQGEL R+ YKV IP+ K+  VNQ  N + PS+KY++VVT
Sbjct: 129 LFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYLEVVT 188

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           VD FDFWFMGFL+Y+KA   L++ +
Sbjct: 189 VDGFDFWFMGFLSYQKAFNCLEQAL 213

[Close]

BLAST of Lsi04G007860 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 174.1 bits (440), Expect = 1.5e-43
Identity = 79/145 (54.48%), Postives = 110/145 (75.86%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           KL P + +TVK KL LG K++Q GG EKI++++F +   +KL  + QCYLSTTAGPIAGL
Sbjct: 131 KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGL 190

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I ++SPQG L R+ YKV IP+ K+  VNQ  N   PS+KY+++VT
Sbjct: 191 LFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKKPSQKYLEIVT 250

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           +D+FDFWFMGF++Y+KA   L+K +
Sbjct: 251 IDNFDFWFMGFVSYQKAFNCLEKAL 275

[Close]

BLAST of Lsi04G007860 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 168.3 bits (425), Expect = 8.4e-42
Identity = 77/145 (53.10%), Postives = 109/145 (75.17%), Query Frame = 0


Query: 121 KLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAGL 180
           KL P + +TVK KL LG K++Q GG EKI++++F +   +KL  + QCYLSTT G IAGL
Sbjct: 61  KLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCYLSTTEGSIAGL 120

Query: 181 LFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVVT 240
           LF+S+  +AFCS+R I ++SPQG+L R+ YKV IP+ K+  VNQ  N   PS++Y++VVT
Sbjct: 121 LFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQNTKKPSQRYLEVVT 180

Query: 241 VDDFDFWFMGFLNYEKAVKFLQKGV 266
           VD++DFWFMGF++Y+KA   L+K +
Sbjct: 181 VDNYDFWFMGFVSYQKAFNCLEKAL 205

[Close]

BLAST of Lsi04G007860 vs. TAIR 10
Match: AT1G28200.1 (FH interacting protein 1 )

HSP 1 Score: 132.1 bits (331), Expect = 6.6e-31
Identity = 63/148 (42.57%), Postives = 98/148 (66.22%), Query Frame = 0


Query: 120 IKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFQKMFNLRQGDKLLSSAQCYLSTTAGPIAG 179
           +K  P++A     ++  GTK++  GG EK+F++ F+    +KLL +  CYLST+AGP+ G
Sbjct: 110 LKTGPSVADAAVSRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKTYACYLSTSAGPVLG 169

Query: 180 LLFVSTHVVAFCSDRPIIISSPQGELGRLFYKVMIPVKKVKRVNQRNNVSNPSKKYIQVV 239
           ++++STH +AF SD P+     +  L   +YKV++P  ++K VN   +  N S KYIQV+
Sbjct: 170 VMYLSTHKLAFSSDNPLSYKEGEQTLWS-YYKVVLPANQLKAVNPSTSRVNTSDKYIQVI 229

Query: 240 TVDDFDFWFMGFLNYEKAVKFLQKGVFS 268
           ++D+ +FWFMGF+ YE AVK LQ+ V S
Sbjct: 230 SIDNHEFWFMGFVTYESAVKSLQEAVQS 256

The following BLAST results are available for this feature:

BLAST of Lsi04G007860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FTA0	1.5e-43	56.55	GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1	[more]
Q9FMW4	4.3e-43	55.86	Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV...	[more]
Q9FMW6	2.1e-42	54.48	GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2	[more]
Q9FMW5	1.2e-40	53.10	GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1	[more]
Q9SE96	9.3e-30	42.57	GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1	[more]

BLAST of Lsi04G007860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SSY1	1.4e-68	88.36	GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00...	[more]
A0A1S3BDR8	1.4e-68	88.36	GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488777 PE=3 SV=1	[more]
A0A0A0KPM0	2.0e-67	86.30	GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G577990 PE=3 S...	[more]
A0A6J1CD83	1.2e-64	85.62	putative GEM-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111010135 PE=3 ...	[more]
A0A6J1JHW6	3.8e-58	66.67	GEM-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111487132 PE=3 SV=1	[more]

BLAST of Lsi04G007860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893009.1	2.8e-71	93.33	GEM-like protein 4 [Benincasa hispida]	[more]
XP_008445893.1	2.9e-68	88.36	PREDICTED: GEM-like protein 4 [Cucumis melo]	[more]
KAA0034080.1	2.9e-68	88.36	GEM-like protein 4 [Cucumis melo var. makuwa]	[more]
XP_004147134.1	4.1e-67	86.30	GEM-like protein 4 [Cucumis sativus] >KGN51560.1 hypothetical protein Csa_008945...	[more]
XP_022139162.1	2.5e-64	85.62	putative GEM-like protein 8 [Momordica charantia]	[more]

BLAST of Lsi04G007860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08350.1	1.1e-44	56.55	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	3.1e-44	55.86	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	1.5e-43	54.48	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	8.4e-42	53.10	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT1G28200.1	6.6e-31	42.57	FH interacting protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	SMART	SM00568	gram2001c	coord: 148..226 e-value: 1.2E-17 score: 74.6
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 149..263 e-value: 2.4E-19 score: 69.3
IPR011993	PH-like domain superfamily	GENE3D	2.30.29.30		coord: 145..265 e-value: 1.0E-20 score: 75.6
IPR037848	GEM-like protein	PANTHER	PTHR31969	GEM-LIKE PROTEIN 2	coord: 119..265
None	No IPR available	PANTHER	PTHR31969:SF38	GEM-LIKE PROTEIN 4	coord: 119..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G007860.1	Lsi04G007860.1	mRNA