Lsi04G007830 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G007830
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGEM-like protein 4
Locationchr04: 7367201 .. 7368682 (+)
RNA-Seq ExpressionLsi04G007830
SyntenyLsi04G007830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGGGAAATCCTTAATTTACGAAACATGCATGGGCTCCTTTGTCATTCCATAAAAGTTGATGATGAGGCAGAGATGACTCATAGCTGACTAATTAAAGCCAAAAGCAATTTTAATTTTATATCATTCATATACCAAACTTTAATTTTCTTTTCCCTTTAAATACATTAACTCAATTCATTCATCCCACCAAAATTCAAACCTCTATACATTCCTAAATCTCTCGATTTTCTATCTTTTTCTTCAATTTGAAAAAAAAAAAATGAAGACTACAATTCAAGAACATATGTTGGGAATTCAGATGTCATTGGCTCTTGCAAGAGCTAGATATTTGGGTGCTCCTTCAAGGCATCTCCATATATCTTCTTCAGATGATGGATCTCAATCAATTAAGAAATGTAAGAGCTATATAAGAAGAAGAAGAAAGAAAGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTATGAATATATAAATTGTTTTGCTAACTAATTTGGTTTTTGAGTTGTTGCAGATAATGGAAAAGATTGGATCCTCAACAAAAATGGAAGAAAAACAAACAATATCATTCATGCAATCCGAGAACATGGTAAATCTTCTATAGAGATTCGAGAGTCCTTAATTCATCCAACAACTCTAGAATTTTAAATTAGGATTTAAAAGCTATCCTCAAACACGTAAGTGGTAAGAATAGTTTTCACTCTAACAAACAACATGATTAAGTTTGGGTACGATGAGAATTTACGTTAATTACCGAATTTGGTTAGTTATATAAATATATATGGTTGAATTATTTGTGATGCTTTTGGAGTTGGATTTGCAGTGAAATTAGGAGCCAAGATATCAGAAACAGTAAAGGGAAAGCTGAGTTTAGGAGCAAGAATTTTGAGAGTTGGTGGATTGAGGAAAATTTACAAGAAATTATTTTCAGTGAGTGAAGGAGAAAAGCTTTTAAAGGCTTCACAATGCTATTTATCAACAACAGCTGGGCCTTTGGCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGCAGTGATAAATCCATCAAACTCTCTTCCCCAAATGGAGATGATGATCATGTTAGAATTCACTATAAAGTAAGCTTCTTTTTATATATAATGTATGTAATTAAATTAGGGTAATAATATTGAATGAATTTAATTTTGCAGGTTGTGATTCCAAAGGAGAAGGTGAAGAGAGTAAATGAAAGTGAGAATAATGTTAAGAAAGATTCAGAAAAATACATACAAATAGTGACAATGGACAACTTTGAGTTTTGGTTTATGGGTTTTCTAAATTATCAATCAACTTTCAACTCTCTTCAGGGAGTAGACAGAAGACTGAGCTAGATGATGCTAAATTATTATATGTATTATATTTCAATTTGAATCATGAAACATTGATTTTTGTACAGATTTATTCAATCTAATTAATGAGAAAATTCAATTTGAGATATAATGCTCATCTTTTTTTTGA

mRNA sequence

AAAAAGGGAAATCCTTAATTTACGAAACATGCATGGGCTCCTTTGTCATTCCATAAAAGTTGATGATGAGGCAGAGATGACTCATAGCTGACTAATTAAAGCCAAAAGCAATTTTAATTTTATATCATTCATATACCAAACTTTAATTTTCTTTTCCCTTTAAATACATTAACTCAATTCATTCATCCCACCAAAATTCAAACCTCTATACATTCCTAAATCTCTCGATTTTCTATCTTTTTCTTCAATTTGAAAAAAAAAAAATGAAGACTACAATTCAAGAACATATGTTGGGAATTCAGATGTCATTGGCTCTTGCAAGAGCTAGATATTTGGGTGCTCCTTCAAGGCATCTCCATATATCTTCTTCAGATGATGGATCTCAATCAATTAAGAAATATAATGGAAAAGATTGGATCCTCAACAAAAATGGAAGAAAAACAAACAATATCATTCATGCAATCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTAAAGGGAAAGCTGAGTTTAGGAGCAAGAATTTTGAGAGTTGGTGGATTGAGGAAAATTTACAAGAAATTATTTTCAGTGAGTGAAGGAGAAAAGCTTTTAAAGGCTTCACAATGCTATTTATCAACAACAGCTGGGCCTTTGGCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGCAGTGATAAATCCATCAAACTCTCTTCCCCAAATGGAGATGATGATCATGTTAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGAAGAGAGTAAATGAAAGTGAGAATAATGTTAAGAAAGATTCAGAAAAATACATACAAATAGTGACAATGGACAACTTTGAGTTTTGGTTTATGGGTTTTCTAAATTATCAATCAACTTTCAACTCTCTTCAGGGAGTAGACAGAAGACTGAGCTAGATGATGCTAAATTATTATATGTATTATATTTCAATTTGAATCATGAAACATTGATTTTTGTACAGATTTATTCAATCTAATTAATGAGAAAATTCAATTTGAGATATAATGCTCATCTTTTTTTTGA

Coding sequence (CDS)

ATGAAGACTACAATTCAAGAACATATGTTGGGAATTCAGATGTCATTGGCTCTTGCAAGAGCTAGATATTTGGGTGCTCCTTCAAGGCATCTCCATATATCTTCTTCAGATGATGGATCTCAATCAATTAAGAAATATAATGGAAAAGATTGGATCCTCAACAAAAATGGAAGAAAAACAAACAATATCATTCATGCAATCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTAAAGGGAAAGCTGAGTTTAGGAGCAAGAATTTTGAGAGTTGGTGGATTGAGGAAAATTTACAAGAAATTATTTTCAGTGAGTGAAGGAGAAAAGCTTTTAAAGGCTTCACAATGCTATTTATCAACAACAGCTGGGCCTTTGGCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGCAGTGATAAATCCATCAAACTCTCTTCCCCAAATGGAGATGATGATCATGTTAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGAAGAGAGTAAATGAAAGTGAGAATAATGTTAAGAAAGATTCAGAAAAATACATACAAATAGTGACAATGGACAACTTTGAGTTTTGGTTTATGGGTTTTCTAAATTATCAATCAACTTTCAACTCTCTTCAGGGAGTAGACAGAAGACTGAGCTAG

Protein sequence

MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILNKNGRKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNESENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDRRLS
Homology
BLAST of Lsi04G007830 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-49
Identity = 98/159 (61.64%), Postives = 128/159 (80.50%), Query Frame = 0

Query: 58  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 117
           +KT+   + +R+  K+  K++ETVK KLSLGARIL+VGGL KI+K+LF VSEGEKL K  
Sbjct: 54  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 113

Query: 118 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 177
           QCYLSTTAGP+AGLLFIS+ K+AFCS++SIK+ SP G  D +R+HYKV IP  K+ RVN+
Sbjct: 114 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQG--DIIRVHYKVSIPLCKIDRVNQ 173

Query: 178 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           S+ N KK S+KY+++VT+D F+FWFMGFL+YQ  FN L+
Sbjct: 174 SQ-NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 209

BLAST of Lsi04G007830 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 8.2e-48
Identity = 104/196 (53.06%), Postives = 138/196 (70.41%), Query Frame = 0

Query: 21  ARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILNKNGRKTNNIIHAIREHVKLGAKISET 80
           A YL  P+    +        S     GK  +  K   K ++  + +R+  KLG K++ET
Sbjct: 21  AGYLPDPASINKLQIPTSSKFSFLTGKGKSMLRKK---KNDSFTNGVRDQDKLGPKLTET 80

Query: 81  VKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKASQCYLSTTAGPLAGLLFISTHKIA 140
           VK KLSLGARIL++GGL KIYK+LF VS+ EKL KA QCYLSTTAGP+AGLLFIS+ KIA
Sbjct: 81  VKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIA 140

Query: 141 FCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNESENNVKKDSEKYIQIVTMDNFEF 200
           FCS++SIK++SP G+ +  R+HYKV IP  K+  VN+S+N   K S+KY+++VT+D F+F
Sbjct: 141 FCSERSIKVASPQGELN--RVHYKVSIPLCKINGVNQSQNTT-KPSQKYLEVVTVDGFDF 200

Query: 201 WFMGFLNYQSTFNSLQ 217
           WFMGFL+YQ  FN L+
Sbjct: 201 WFMGFLSYQKAFNCLE 210

BLAST of Lsi04G007830 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 1.8e-47
Identity = 97/159 (61.01%), Postives = 127/159 (79.87%), Query Frame = 0

Query: 58  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 117
           +KT++  +  R+  KLG K++ETVK KLSLGA+IL++GGL KIYK+LF V + EKL KA 
Sbjct: 55  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 114

Query: 118 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 177
           QCYLSTTAGP+AGLLFIS+ KIAFCS++SIK++SP G     R+HYKV IP  K+  VN+
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLS--RVHYKVSIPLCKINGVNQ 174

Query: 178 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           S+ N KK S+KY++IVT+DNF+FWFMGF++YQ  FN L+
Sbjct: 175 SQ-NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLE 210

BLAST of Lsi04G007830 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-46
Identity = 93/159 (58.49%), Postives = 126/159 (79.25%), Query Frame = 0

Query: 58  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 117
           +KT++  +  R+  KLG K++ETVK KLSLGA+IL++GGL KIYK+LF V + EKL KA 
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 118 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 177
           QCYLSTT G +AGLLFIS+ KIAFCS++SIK++SP G  D  R+HYKV IP  K+  VN+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQG--DLTRVHYKVSIPLCKINGVNQ 166

Query: 178 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           S+ N KK S++Y+++VT+DN++FWFMGF++YQ  FN L+
Sbjct: 167 SQ-NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

BLAST of Lsi04G007830 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.9e-30
Identity = 74/193 (38.34%), Postives = 117/193 (60.62%), Query Frame = 0

Query: 30  HLHISSSDDGSQSIKKYNGKDWILNKNG-------RKTNNIIHAIREHVKLGAKISETVK 89
           ++HI+S    S    K    + +LN+ G       RK   ++  +++H+K    IS+   
Sbjct: 31  YVHITSPTSASDKRSKDKVLE-VLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAM 90

Query: 90  GKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKASQCYLSTTAGPLAGLLFISTHKIAFC 149
            +LS G +++  GG  +++++ F V   EKLL +  CY+STT+GP+ G+++IS  +IAFC
Sbjct: 91  ARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFC 150

Query: 150 SDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNESENNVKKDSEKYIQIVTMDNFEFWF 209
           SD +I+L S  G +  V  +YKVV+  EK+  ++ S  NV K SE+Y+ +VT D FEFWF
Sbjct: 151 SDYAIRLPSSAGGNG-VAAYYKVVMEWEKISSIS-SSTNVLKPSERYVHMVTRDGFEFWF 210

Query: 210 MGFLNYQSTFNSL 216
           MGF++Y   FN L
Sbjct: 211 MGFVSYIDAFNCL 220

BLAST of Lsi04G007830 vs. ExPASy TrEMBL
Match: A0A6J1KB26 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493848 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.3e-93
Identity = 182/223 (81.61%), Postives = 203/223 (91.03%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILN---KNG 60
           MK+ IQ+H+LG+Q+SLALARAR+L  PS+HLH+SS DDGSQSI+KYNGKDWILN   KNG
Sbjct: 1   MKSAIQQHVLGMQLSLALARARFLAHPSKHLHMSSLDDGSQSIQKYNGKDWILNRVHKNG 60

Query: 61  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 120
           RK +NII  +REHVKLGAKI+ETVKGKLSLGARI+RVGG+RKIYKKLF+VSEGEKLLKAS
Sbjct: 61  RKPDNIIRELREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKAS 120

Query: 121 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 180
           QCYLSTT GP+AGLLFISTH IAFCSDKSIKLSSPNG ++HVRIHYKVVIP EKV RVNE
Sbjct: 121 QCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLSSPNG-EEHVRIHYKVVIPNEKVMRVNE 180

Query: 181 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDR 221
           SE NVKK S+KYIQIVT+D FEFWFMGFLNYQSTFNSLQ + R
Sbjct: 181 SE-NVKKASQKYIQIVTVDKFEFWFMGFLNYQSTFNSLQELQR 221

BLAST of Lsi04G007830 vs. ExPASy TrEMBL
Match: A0A6J1KFK0 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493841 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.9e-92
Identity = 184/224 (82.14%), Postives = 200/224 (89.29%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQM-SLALARARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILN---KN 60
           MK TIQEH+ G QM SL LARAR+L  PS+HLH+SS DDG QSIKKYNGKDWILN   KN
Sbjct: 1   MKITIQEHVFGAQMKSLGLARARFLADPSKHLHMSSLDDGYQSIKKYNGKDWILNRVHKN 60

Query: 61  GRKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKA 120
           GRK +NII A+REHVKLGAKI+ETVKGKLSLGARI+RVGG+RKIYKKLF+VSEGEKLLKA
Sbjct: 61  GRKPDNIIRALREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKA 120

Query: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVN 180
           SQCYLSTT GP+AGLLFISTH IAFCSDKSIKLSSPNG ++HVRIHYKVVIP EKV RVN
Sbjct: 121 SQCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLSSPNG-EEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDR 221
           ESE NVKK S+KYIQIVT+D FEFWFMGFLNYQSTFNSLQ + R
Sbjct: 181 ESE-NVKKASQKYIQIVTVDKFEFWFMGFLNYQSTFNSLQELQR 222

BLAST of Lsi04G007830 vs. ExPASy TrEMBL
Match: A0A6J1GXL4 (GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458126 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.9e-92
Identity = 180/223 (80.72%), Postives = 201/223 (90.13%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILN---KNG 60
           MK+ IQ+ +LG+Q+SL LARAR+L  PS+H H+SS DDGSQSI+KYNGKDWILN   KNG
Sbjct: 1   MKSAIQQQVLGMQLSLGLARARFLADPSKHFHMSSLDDGSQSIQKYNGKDWILNRVHKNG 60

Query: 61  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 120
           RK +NII A+REHVKLGAKI+ETVKGKL LGARI+RVGG+RKIYKKLF+VSEGEKLLKAS
Sbjct: 61  RKPDNIIRALREHVKLGAKITETVKGKLRLGARIVRVGGVRKIYKKLFTVSEGEKLLKAS 120

Query: 121 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 180
           QCYLSTTAGP+AGLLFISTH IAFCSDKSIKLSSPNG ++HVRIHYKVVIP EKV RVNE
Sbjct: 121 QCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNG-EEHVRIHYKVVIPNEKVMRVNE 180

Query: 181 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDR 221
           SE NVKK S+KYIQIVT+D FEFWFMGFL YQSTFNSLQG+ R
Sbjct: 181 SE-NVKKASQKYIQIVTVDKFEFWFMGFLKYQSTFNSLQGLQR 221

BLAST of Lsi04G007830 vs. ExPASy TrEMBL
Match: A0A5A7SSL2 (GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00700 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.3e-92
Identity = 187/224 (83.48%), Postives = 201/224 (89.73%), Query Frame = 0

Query: 3   TTIQEHMLGIQMSLALARARYLG-APSRHLHISSSDD--GSQSIKKYNGKDWI---LNKN 62
           TTIQEHMLG QMS AL+RARYLG  PS+HLH+SSSDD     SIKKYNGKD I   LNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GRKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKA 122
           G+KT+NIIHA+REHVKLGAKISETVKGKLSLGARILRVGG+RKIYKKLFS++E EKLLK 
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVN 182
           SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIK++SPN + DH+RIHYKVVIPKEKV RVN
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPN-NGDHIRIHYKVVIPKEKVMRVN 183

Query: 183 ESENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDR 221
           ESE NVKK SEKYIQI T+DNFEFWFMGFLNYQSTFNSLQ V R
Sbjct: 184 ESE-NVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 225

BLAST of Lsi04G007830 vs. ExPASy TrEMBL
Match: A0A1S3BEG1 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.3e-92
Identity = 185/220 (84.09%), Postives = 200/220 (90.91%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDDGSQ-SIKKYNGKDWILNK---N 60
           MKTTIQEHMLG QMSLAL+RARYLG P + LH+SSSDDGSQ SIKKYNGKD ILN+   N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  GRKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKA 120
           G++T+NIIHA+REHVKLG KISE VKGKLSLGA+ILRVGGLRKIYKKLFS++E EKLLK 
Sbjct: 61  GKRTDNIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVN 180
           SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIK++SPNG  DH+RI YKVVIPKEKV RVN
Sbjct: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNG--DHIRILYKVVIPKEKVMRVN 180

Query: 181 ESENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           ESE NVKK SEKYIQI T+DNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 ESE-NVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQ 217

BLAST of Lsi04G007830 vs. NCBI nr
Match: XP_023513129.1 (putative GEM-like protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.9 bits (912), Expect = 2.5e-94
Identity = 187/225 (83.11%), Postives = 204/225 (90.67%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQM-SLALARARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILN---KN 60
           MK TIQEH+LG QM SL LARAR+L  PS+HLH+SSSDDG QSIKKYNGKDWILN   KN
Sbjct: 1   MKITIQEHVLGAQMKSLGLARARFLADPSKHLHMSSSDDGYQSIKKYNGKDWILNRVHKN 60

Query: 61  GRKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKA 120
           GRK +NII A+REHVKLGAKI+ETVKGKLSLGARI+RVGG+RKIYKKLF+VSEGEKLLKA
Sbjct: 61  GRKPDNIIRALREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKA 120

Query: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVN 180
           SQCYLSTTAGP+AGLLFISTH IAFCSDKSIK SSPNG ++HVRIHYKVVIP EKV RVN
Sbjct: 121 SQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKFSSPNG-EEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDRR 222
           ESE NVKK S+KYIQIVT+DNFEFWFMGFLNYQSTFNSLQ + +R
Sbjct: 181 ESE-NVKKASQKYIQIVTVDNFEFWFMGFLNYQSTFNSLQELQKR 223

BLAST of Lsi04G007830 vs. NCBI nr
Match: XP_023513116.1 (putative GEM-like protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.4 bits (903), Expect = 2.8e-93
Identity = 183/223 (82.06%), Postives = 203/223 (91.03%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILN---KNG 60
           MK+ IQ+ +LG+Q+SLALARAR+L  PS+HLHISS DDGSQSI+KYNGKDWILN   KNG
Sbjct: 1   MKSAIQQQVLGMQLSLALARARFLAHPSKHLHISSLDDGSQSIQKYNGKDWILNRVHKNG 60

Query: 61  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 120
           RK +NII A+REHVKLGAKI+ETVKGKLSLGARI+RVGG+RKIYKKLF+VSEGEKLLKAS
Sbjct: 61  RKPDNIIRALREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKAS 120

Query: 121 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 180
           QCYLSTTAGP+AGLLFISTH IAFCSDKSIKLSSPNG ++HVRIHYKVVIP EKV RVNE
Sbjct: 121 QCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNG-EEHVRIHYKVVIPNEKVMRVNE 180

Query: 181 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDR 221
           SE NVKK S+KYIQIVT+D FEFWFMGFLNYQSTFNS Q + R
Sbjct: 181 SE-NVKKASQKYIQIVTVDKFEFWFMGFLNYQSTFNSFQELQR 221

BLAST of Lsi04G007830 vs. NCBI nr
Match: XP_022999512.1 (putative GEM-like protein 8 [Cucurbita maxima])

HSP 1 Score: 352.4 bits (903), Expect = 2.8e-93
Identity = 182/223 (81.61%), Postives = 203/223 (91.03%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILN---KNG 60
           MK+ IQ+H+LG+Q+SLALARAR+L  PS+HLH+SS DDGSQSI+KYNGKDWILN   KNG
Sbjct: 1   MKSAIQQHVLGMQLSLALARARFLAHPSKHLHMSSLDDGSQSIQKYNGKDWILNRVHKNG 60

Query: 61  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 120
           RK +NII  +REHVKLGAKI+ETVKGKLSLGARI+RVGG+RKIYKKLF+VSEGEKLLKAS
Sbjct: 61  RKPDNIIRELREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKAS 120

Query: 121 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 180
           QCYLSTT GP+AGLLFISTH IAFCSDKSIKLSSPNG ++HVRIHYKVVIP EKV RVNE
Sbjct: 121 QCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLSSPNG-EEHVRIHYKVVIPNEKVMRVNE 180

Query: 181 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDR 221
           SE NVKK S+KYIQIVT+D FEFWFMGFLNYQSTFNSLQ + R
Sbjct: 181 SE-NVKKASQKYIQIVTVDKFEFWFMGFLNYQSTFNSLQELQR 221

BLAST of Lsi04G007830 vs. NCBI nr
Match: XP_011655499.1 (GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572 [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 4.7e-93
Identity = 188/225 (83.56%), Postives = 201/225 (89.33%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDD--GSQSIKKYNGKDWI---LNK 60
           MKTTIQEHMLG QMSLAL+RAR LG  S+HLH+SSSDD     SIKKYNGKD I   LNK
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNK 60

Query: 61  NGRKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLK 120
           NG+KT+NIIHA+REHVKLGAKISETVKGKLSLGARILRVGG+RKIYKKLFS+SE EKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRV 180
            SQCYLSTTAGPL GLLFISTHKIAFCSDKSIK++SPNG  DH+RIHYKVVIPKEKV RV
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNG--DHIRIHYKVVIPKEKVMRV 180

Query: 181 NESENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQGVDR 221
           NESE NVKK SE+YIQI T+DNFEFWFMGFLNYQSTFNSLQ V R
Sbjct: 181 NESE-NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQEVAR 222

BLAST of Lsi04G007830 vs. NCBI nr
Match: XP_011655497.2 (putative GEM-like protein 8 [Cucumis sativus])

HSP 1 Score: 350.9 bits (899), Expect = 8.1e-93
Identity = 187/220 (85.00%), Postives = 199/220 (90.45%), Query Frame = 0

Query: 1   MKTTIQEHMLGIQMSLALARARYLGAPSRHLHISSSDDGSQ-SIKKYNGKDWI---LNKN 60
           MKTTIQEHMLG  MSLAL+RARYL  PS  LH+SSSDDG Q SIKKYNGKD I   LNKN
Sbjct: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60

Query: 61  GRKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKA 120
           G+KT+NIIHA+REHVKLGAKISETVKGKLSLGARILRVGG+RKIYKKLFS+SE EKLLK 
Sbjct: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120

Query: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVN 180
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIK++SPNG  DH+RIHYKVVIPKEKV RVN
Sbjct: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNG--DHIRIHYKVVIPKEKVMRVN 180

Query: 181 ESENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           ESE NVKK SE+YIQI T+DNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 ESE-NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 217

BLAST of Lsi04G007830 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 197.6 bits (501), Expect = 1.1e-50
Identity = 98/159 (61.64%), Postives = 128/159 (80.50%), Query Frame = 0

Query: 58  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 117
           +KT+   + +R+  K+  K++ETVK KLSLGARIL+VGGL KI+K+LF VSEGEKL K  
Sbjct: 54  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 113

Query: 118 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 177
           QCYLSTTAGP+AGLLFIS+ K+AFCS++SIK+ SP G  D +R+HYKV IP  K+ RVN+
Sbjct: 114 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQG--DIIRVHYKVSIPLCKIDRVNQ 173

Query: 178 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           S+ N KK S+KY+++VT+D F+FWFMGFL+YQ  FN L+
Sbjct: 174 SQ-NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 209

BLAST of Lsi04G007830 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 191.8 bits (486), Expect = 5.8e-49
Identity = 104/196 (53.06%), Postives = 138/196 (70.41%), Query Frame = 0

Query: 21  ARYLGAPSRHLHISSSDDGSQSIKKYNGKDWILNKNGRKTNNIIHAIREHVKLGAKISET 80
           A YL  P+    +        S     GK  +  K   K ++  + +R+  KLG K++ET
Sbjct: 21  AGYLPDPASINKLQIPTSSKFSFLTGKGKSMLRKK---KNDSFTNGVRDQDKLGPKLTET 80

Query: 81  VKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKASQCYLSTTAGPLAGLLFISTHKIA 140
           VK KLSLGARIL++GGL KIYK+LF VS+ EKL KA QCYLSTTAGP+AGLLFIS+ KIA
Sbjct: 81  VKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIA 140

Query: 141 FCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNESENNVKKDSEKYIQIVTMDNFEF 200
           FCS++SIK++SP G+ +  R+HYKV IP  K+  VN+S+N   K S+KY+++VT+D F+F
Sbjct: 141 FCSERSIKVASPQGELN--RVHYKVSIPLCKINGVNQSQNTT-KPSQKYLEVVTVDGFDF 200

Query: 201 WFMGFLNYQSTFNSLQ 217
           WFMGFL+YQ  FN L+
Sbjct: 201 WFMGFLSYQKAFNCLE 210

BLAST of Lsi04G007830 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 190.7 bits (483), Expect = 1.3e-48
Identity = 97/159 (61.01%), Postives = 127/159 (79.87%), Query Frame = 0

Query: 58  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 117
           +KT++  +  R+  KLG K++ETVK KLSLGA+IL++GGL KIYK+LF V + EKL KA 
Sbjct: 117 KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 176

Query: 118 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 177
           QCYLSTTAGP+AGLLFIS+ KIAFCS++SIK++SP G     R+HYKV IP  K+  VN+
Sbjct: 177 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLS--RVHYKVSIPLCKINGVNQ 236

Query: 178 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           S+ N KK S+KY++IVT+DNF+FWFMGF++YQ  FN L+
Sbjct: 237 SQ-NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLE 272

BLAST of Lsi04G007830 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 187.2 bits (474), Expect = 1.4e-47
Identity = 93/159 (58.49%), Postives = 126/159 (79.25%), Query Frame = 0

Query: 58  RKTNNIIHAIREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKAS 117
           +KT++  +  R+  KLG K++ETVK KLSLGA+IL++GGL KIYK+LF V + EKL KA 
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 118 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNE 177
           QCYLSTT G +AGLLFIS+ KIAFCS++SIK++SP G  D  R+HYKV IP  K+  VN+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQG--DLTRVHYKVSIPLCKINGVNQ 166

Query: 178 SENNVKKDSEKYIQIVTMDNFEFWFMGFLNYQSTFNSLQ 217
           S+ N KK S++Y+++VT+DN++FWFMGF++YQ  FN L+
Sbjct: 167 SQ-NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

BLAST of Lsi04G007830 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 132.5 bits (332), Expect = 4.2e-31
Identity = 74/193 (38.34%), Postives = 117/193 (60.62%), Query Frame = 0

Query: 30  HLHISSSDDGSQSIKKYNGKDWILNKNG-------RKTNNIIHAIREHVKLGAKISETVK 89
           ++HI+S    S    K    + +LN+ G       RK   ++  +++H+K    IS+   
Sbjct: 31  YVHITSPTSASDKRSKDKVLE-VLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAM 90

Query: 90  GKLSLGARILRVGGLRKIYKKLFSVSEGEKLLKASQCYLSTTAGPLAGLLFISTHKIAFC 149
            +LS G +++  GG  +++++ F V   EKLL +  CY+STT+GP+ G+++IS  +IAFC
Sbjct: 91  ARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFC 150

Query: 150 SDKSIKLSSPNGDDDHVRIHYKVVIPKEKVKRVNESENNVKKDSEKYIQIVTMDNFEFWF 209
           SD +I+L S  G +  V  +YKVV+  EK+  ++ S  NV K SE+Y+ +VT D FEFWF
Sbjct: 151 SDYAIRLPSSAGGNG-VAAYYKVVMEWEKISSIS-SSTNVLKPSERYVHMVTRDGFEFWF 210

Query: 210 MGFLNYQSTFNSL 216
           MGF++Y   FN L
Sbjct: 211 MGFVSYIDAFNCL 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FTA01.5e-4961.64GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1[more]
Q9FMW48.2e-4853.06Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV... [more]
Q9FMW61.8e-4761.01GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2[more]
Q9FMW52.0e-4658.49GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1[more]
Q9M1225.9e-3038.34GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KB261.3e-9381.61putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493848 PE=3 SV=... [more]
A0A6J1KFK01.9e-9282.14putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493841 PE=3 SV=... [more]
A0A6J1GXL41.9e-9280.72GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458126 PE=3 SV=1[more]
A0A5A7SSL23.3e-9283.48GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00... [more]
A0A1S3BEG13.3e-9284.09GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023513129.12.5e-9483.11putative GEM-like protein 8 [Cucurbita pepo subsp. pepo][more]
XP_023513116.12.8e-9382.06putative GEM-like protein 8 [Cucurbita pepo subsp. pepo][more]
XP_022999512.12.8e-9381.61putative GEM-like protein 8 [Cucurbita maxima][more]
XP_011655499.14.7e-9383.56GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572... [more]
XP_011655497.28.1e-9385.00putative GEM-like protein 8 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G08350.11.1e-5061.64GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23370.15.8e-4953.06GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23350.11.3e-4861.01GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23360.11.4e-4758.49GRAM domain-containing protein / ABA-responsive protein-related [more]
AT4G01600.14.2e-3138.34GRAM domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 99..179
e-value: 4.5E-19
score: 79.4
IPR004182GRAM domainPFAMPF02893GRAMcoord: 102..216
e-value: 2.4E-17
score: 62.9
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 97..219
e-value: 1.7E-20
score: 74.8
NoneNo IPR availablePANTHERPTHR31969:SF38GEM-LIKE PROTEIN 4coord: 1..217
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 1..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G007830.1Lsi04G007830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity