Lsi04G007820 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G007820
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	GRAM domain-containing protein
Location	chr04: 7361612 .. 7364601 (+)
RNA-Seq Expression	Lsi04G007820
Synteny	Lsi04G007820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTATCTTCCTTTAAATAGCTTCACCCACTTCAATTCCAATTCCCATCACTCTGTTTTTTTCTTCTTTCTTCTCTAATTAAATCTTAATGGATAGTTCATTTCAAGCTGAGCAAATGGTCATCGGAGCTCCGTTCAGGTTGCCAGCGGCTTTGGGGAGGCTCCGGCATGATTCTGTGGCTAAGTTGATGATGGAAGAATATAATGGCTTCAAATCAATGAAACAATGTAAGAATTCAAATGAGATGAAAAATAATATTAGCATTTTTATTTTATTTTTTTCTGTTGAATATTATAATTAATGAACAAAAAGAATTGATGGGTTTGACGTGAAAATTGCAGATAGTAGGAAAAATGATTGTTCATTAAGAAACCGAGTGAGCAAGATGAGAAGAAAAGCTGATGGCTTTGTTCATGGGATTGGAGACCATTGTAAGTCCAGTTAGTTTTGAAATGAAACTTCGAGGTGTTTGGTTTAATTTTTCAAAAGTTTAATTTTGAAAATAAGACATTTTAGAAAAAATTGAAGTGTTTTGACACCCACTCAAAATAGTTTTTGAAATATTTTCAAAACGTATTTTAAACAATTTTTATCAAAAGAGTTTAAAGAAAAATAATTTTTTGAAAAACATTTTTTCTCTAGTCATTACAGACAGACCCTTCATGTTTTCTCTAAGAGTATTCGGTCCAAATTGTTATAAATTTTAACAATATATTTTTCAAAATAGAGACTGAATTGTTATAAATTTTAAAATAAAAGAAACCAAATTGTTACATATTAAATTCAAATGACTAAATTGTTACAAAATTAAAAGTACACAAATTGAATTGTTACAAAATCAAAAAGTTTCAAAGATTAAATTGTTACTTCTATGAAAGTATATATAAGAACACAATTTATAATAATTAGAGATCTAATTAGAGTTTATATACAAGAATGAGATTTTCAACTAACATGAAACTTAATTTCAAAATTATTAGACTAAACTAATTAAAGAAGTAAACTTGATAAGATATATAATAATAAACCCTAAATTCTAACACTTTTTTGGGACATGGGTTTTGTCAAATTTAATTAGTTTTTGATGTTTAATGATATTATATTGAAAAATGCAGTGAGATTGGGGCCTAAGATGACAGAGACAGTGAAGGGAAAGTTGAGATTAGGATTAAGAATAGTGAAAATGGGTGGATTGAGAAAAGTTTATGAAAAGTTATTTGAAGAAGTGATGAAGAACAAAGGAGAAGATCAATTGGTTAAGGCTTCCCATTGCTATTTATCAACCACAATTGGTCCTATTGCTGGCCTTCTCTTCATCTCCACTCATCATATTGCTTTTTATAGTGACACACCCATCAAAATCTCTTCTCCAAATGGACAATATCATTTCAAATTCCATTACAAGGTAATTATAAACTCTCTCTTATCAAGATGATATATGTAAAGGTAATTATAATGAGTAGCAATTTTAAGGATAATAATCAAATATATAGCAACATTTTAAACAAAATTGCAAATATAGCAAAGTCTATCAATAATAGACTCCGATAGTCTATCAACGATAAAAGTCTGTTACTGATAGACTCTTATGGTCTATCAGTGATAGACTACCTAGACCATAAAAGTCTATTATTGATAGACTCTTATGGTCTATCAGTGATAGATTACCTAAACCATAAAAGTCTATCAGCGATATAAGTCTATCAGTGATAGACTTTGCTATATTTGCAATTTTTTTAAATGTTGCGCCATCTATGCTAATACTTTGAATCTGATTACTATATTTGTAATTGTCCCTATATGTAAGCTAGGTTAAGGGTCCGTTTGGATTGACTTTCTAGGTGTTTAAATAAGTGTGTATAAGTGCAAGAAAAATGTTTATAAACATACGTAAAAAGTCAATCCAAACGGACCCTTAATTATAGATTTATAGGCATAACTTCAATTTATAAGTTTAAAGACTAGTCAAATCTCGTAAGAGTTGATAACGATTTCTTTTTGTGTTTTTTGTTCTTGAAAAATTGTGATTGTTTTTTTACCGTAAAAAACACTTAGGTGTTGTCTGAACTGCACTTATCTTTGTGCAACCAATAAAAAATATAATTGTTATATAACAATTTACTTTTGTGTGTAAAAGAAAGGGGAGCATGAGATTAAGTACAACTATTATTATTATTTTTTTTACTATGTGGTGTGTTTATCCCTTTTAAAGAAACAATTGGCCAATTGGGTTCTTTATTTAGCCAAATTCTAAAAAAACAGTAATAATTTTTTTCTTAAAAAATCTATTTCTTCCAATTTTCAAACCTTGAATGAGAGGTTTTGAAAATATTTTTACGAGAGCAAGAGGAAAGAAACTCATAGGCAAAAGTAGTATTCATAAGTTAAATTTTTAAAAAAGAAAAAAAAAATCAAATTACACCAAATGGTGTTATTAATTTAGTGTCAAACCTCATAATTTTATTTATTTATTTACTTATTTGCATTTTAGCATGTTGAATACATTTTGAGATATTTATTTAAATAGATACATTTTTAAATTATAAAATTAATTTGAATTTAATCTAATATTATTATTATATATGCATGTGGTGGATGTGCAGGTTGTGATTCCAAAGGAGAATGTGATGAGAGTGAATGAAAGTGAGAATGTTAAGAAAGCTTCAGAAAAGTACATACAAATAGTGACAATGGACAACTTTGAATTTTGGTTTATGGGTTTTCTAAATTATCAATCAACTTTCAAATGTCTTAATAAGGGAGTAATAGACAGAAAAGTTTGAACTTTTGTTTTTTTTTAATGCCTCAAATTAATATTTGATTATTTGTACAGATTAATCCAATCTAATTAATGAAAAAAAATTACCATTTTGATTATATTCCAAACTTTGATCATCTTTTTGTTCTACTAAGTAGAATATTGATCACCATTTTATTTCATATGTGTTCGACTAGGTCAATAAACTTGTTATATATCTCAAGAACTTTG

mRNA sequence

CTTTTATCTTCCTTTAAATAGCTTCACCCACTTCAATTCCAATTCCCATCACTCTGTTTTTTTCTTCTTTCTTCTCTAATTAAATCTTAATGGATAGTTCATTTCAAGCTGAGCAAATGGTCATCGGAGCTCCGTTCAGGTTGCCAGCGGCTTTGGGGAGGCTCCGGCATGATTCTGTGGCTAAGTTGATGATGGAAGAATATAATGGCTTCAAATCAATGAAACAATTGAGATTGGGGCCTAAGATGACAGAGACAGTGAAGGGAAAGTTGAGATTAGGATTAAGAATAGTGAAAATGGGTGGATTGAGAAAAGTTTATGAAAAGTTATTTGAAGAAGTGATGAAGAACAAAGGAGAAGATCAATTGGTTGTGATTCCAAAGGAGAATGTGATGAGAGTGAATGAAAGTGAGAATGTTAAGAAAGCTTCAGAAAAGTACATACAAATAGTGACAATGGACAACTTTGAATTTTGGTTTATGGGTTTTCTAAATTATCAATCAACTTTCAAATGTCTTAATAAGGGAGTAATAGACAGAAAAGTTTGAACTTTTGTTTTTTTTTAATGCCTCAAATTAATATTTGATTATTTGTACAGATTAATCCAATCTAATTAATGAAAAAAAATTACCATTTTGATTATATTCCAAACTTTGATCATCTTTTTGTTCTACTAAGTAGAATATTGATCACCATTTTATTTCATATGTGTTCGACTAGGTCAATAAACTTGTTATATATCTCAAGAACTTTG

Coding sequence (CDS)

ATGGATAGTTCATTTCAAGCTGAGCAAATGGTCATCGGAGCTCCGTTCAGGTTGCCAGCGGCTTTGGGGAGGCTCCGGCATGATTCTGTGGCTAAGTTGATGATGGAAGAATATAATGGCTTCAAATCAATGAAACAATTGAGATTGGGGCCTAAGATGACAGAGACAGTGAAGGGAAAGTTGAGATTAGGATTAAGAATAGTGAAAATGGGTGGATTGAGAAAAGTTTATGAAAAGTTATTTGAAGAAGTGATGAAGAACAAAGGAGAAGATCAATTGGTTGTGATTCCAAAGGAGAATGTGATGAGAGTGAATGAAAGTGAGAATGTTAAGAAAGCTTCAGAAAAGTACATACAAATAGTGACAATGGACAACTTTGAATTTTGGTTTATGGGTTTTCTAAATTATCAATCAACTTTCAAATGTCTTAATAAGGGAGTAATAGACAGAAAAGTTTGA

Protein sequence

MDSSFQAEQMVIGAPFRLPAALGRLRHDSVAKLMMEEYNGFKSMKQLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLFEEVMKNKGEDQLVVIPKENVMRVNESENVKKASEKYIQIVTMDNFEFWFMGFLNYQSTFKCLNKGVIDRKV

Homology

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BLAST of Lsi04G007820 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.1e-18
Identity = 59/179 (32.96%), Postives = 91/179 (50.84%), Query Frame = 0


Query: 19  PAALGRLRHDSVAKLMMEEYNGFKSM-----KQLRLGPKMTETVKGKLRLGLRIVKMGGL 78
           PA++ +L+  + +K+ M +     S       Q +LGPK+TETVK KL LG +I++MGGL
Sbjct: 27  PASINKLQIPTSSKVSMLQKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGL 86

Query: 79  RKVYEKLFEEVMKNK------------------------------GEDQL---------- 138
            K+Y++LF+   K K                               E  +          
Sbjct: 87  EKIYKRLFKVCDKEKLFKAYQCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLT 146

Query: 139 -----VVIPKENVMRVNESENVKKASEKYIQIVTMDNFEFWFMGFLNYQSTFKCLNKGV 148
                V IP   +  VN+S+N KK S++Y+++VT+DN++FWFMGF++YQ  F CL K +
Sbjct: 147 RVHYKVSIPLCKINGVNQSQNTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKAL 205

[Close]

BLAST of Lsi04G007820 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.0e-17
Identity = 59/180 (32.78%), Postives = 93/180 (51.67%), Query Frame = 0


Query: 16  FRLPAALGRLRHDSVAKLM-MEEYNGFKS--MKQLRLGPKMTETVKGKLRLGLRIVKMGG 75
           FR+PA+  +    +V  ++  ++ +GF +    Q ++ PK+TETVK KL LG RI+++GG
Sbjct: 33  FRVPASSKKSEQSNVKSILKRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGG 92

Query: 76  LRKVYEKLFE-----------------------------------------EVMKNKGE- 135
           L K++++LF                                          +V   +G+ 
Sbjct: 93  LEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDI 152

Query: 136 ---DQLVVIPKENVMRVNESENVKKASEKYIQIVTMDNFEFWFMGFLNYQSTFKCLNKGV 148
                 V IP   + RVN+S+N KK S+KY+++VT+D F+FWFMGFL+YQ  F CL K +
Sbjct: 153 IRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKAL 212

[Close]

BLAST of Lsi04G007820 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-17
Identity = 55/147 (37.41%), Postives = 77/147 (52.38%), Query Frame = 0


Query: 46  QLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLFEEVMKNK----------------- 105
           Q +LGPK+TETVK KL LG +I++MGGL K+Y++LF+   + K                 
Sbjct: 67  QDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIA 126

Query: 106 -------------GEDQL---------------VVIPKENVMRVNESENVKKASEKYIQI 148
                         E  +               V IP   +  VN+S+N KK S+KY++I
Sbjct: 127 GLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKKPSQKYLEI 186

[Close]

BLAST of Lsi04G007820 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 6.7e-17
Identity = 54/147 (36.73%), Postives = 76/147 (51.70%), Query Frame = 0


Query: 46  QLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLFE----------------------- 105
           Q +LGPK+TETVK KL LG RI++MGGL K+Y++LF+                       
Sbjct: 67  QDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIA 126

Query: 106 ------------------EVMKNKGE----DQLVVIPKENVMRVNESENVKKASEKYIQI 148
                             +V   +GE       V IP   +  VN+S+N  K S+KY+++
Sbjct: 127 GLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYLEV 186

[Close]

BLAST of Lsi04G007820 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-09
Identity = 27/58 (46.55%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 94  VVIPKENVMRVNESENVKKASEKYIQIVTMDNFEFWFMGFLNYQSTFKCLNKGVIDRK 152
           VV+  E +  ++ S NV K SE+Y+ +VT D FEFWFMGF++Y   F CLNK +++ +
Sbjct: 171 VVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAFNCLNKALLNSR 228

[Close]

BLAST of Lsi04G007820 vs. ExPASy TrEMBL
Match: A0A6J1CBK1 (GEM-like protein 4 OS=Momordica charantia OX=3673 GN=LOC111010140 PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 2.2e-26
Identity = 95/220 (43.18%), Postives = 113/220 (51.36%), Query Frame = 0


Query: 1   MDSSFQAEQMVIGAPFRLPAALGRLR---HDSVAKLMMEEYNGFKSMKQ----------- 60
           M SS+Q   M  G PFR  AAL R R     S AKLM++ + G KS+KQ           
Sbjct: 1   MSSSYQEHMM--GVPFRSLAALTRRRLLPDHSAAKLMVKGHVGSKSLKQYNGKDHSLRNR 60

Query: 61  ------------------LRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EE 120
                             +RLGPK+TETVKGKL LG RIVK+GGLRKVYE +F     E+
Sbjct: 61  VNKLGRKADSFAHGFREHMRLGPKITETVKGKLSLGARIVKVGGLRKVYENMFRVSQGEK 120

Query: 121 VMK-----------------------------------NKGEDQL-----VVIPKENVMR 144
           ++K                                   +   D +     VVIP   VMR
Sbjct: 121 LLKASHCYLSTTAGPIAGLLFISTHKIAFCSDRSLKFSSPSGDHIRFHYKVVIPVGKVMR 180

[Close]

BLAST of Lsi04G007820 vs. ExPASy TrEMBL
Match: A0A6J1KFK0 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493841 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 71/147 (48.30%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 45  KQLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EEVMK------------- 104
           + ++LG K+TETVKGKL LG RIV++GG+RK+Y+KLF     E+++K             
Sbjct: 73  EHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTGGPM 132

Query: 105 -----------------------NKGEDQL-----VVIPKENVMRVNESENVKKASEKYI 146
                                    GE+ +     VVIP E VMRVNESENVKKAS+KYI
Sbjct: 133 AGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYI 192

[Close]

BLAST of Lsi04G007820 vs. ExPASy TrEMBL
Match: A0A6J1KB26 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493848 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 71/147 (48.30%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 45  KQLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EEVMK------------- 104
           + ++LG K+TETVKGKL LG RIV++GG+RK+Y+KLF     E+++K             
Sbjct: 72  EHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLKASQCYLSTTGGPM 131

Query: 105 -----------------------NKGEDQL-----VVIPKENVMRVNESENVKKASEKYI 146
                                    GE+ +     VVIP E VMRVNESENVKKAS+KYI
Sbjct: 132 AGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVNESENVKKASQKYI 191

[Close]

BLAST of Lsi04G007820 vs. ExPASy TrEMBL
Match: A0A5A7SSL2 (GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00700 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 71/147 (48.30%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 45  KQLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EEVMK------------- 104
           + ++LG K++ETVKGKL LG RI+++GG+RK+Y+KLF     E+++K             
Sbjct: 76  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKVSQCYLSTTAGPL 135

Query: 105 ------------------------NKGE----DQLVVIPKENVMRVNESENVKKASEKYI 146
                                   N G+       VVIPKE VMRVNESENVKKASEKYI
Sbjct: 136 AGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNESENVKKASEKYI 195

[Close]

BLAST of Lsi04G007820 vs. ExPASy TrEMBL
Match: A0A1S3CNX8 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-22
Identity = 71/147 (48.30%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 45  KQLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EEVMK------------- 104
           + ++LG K++ETVKGKL LG RI+++GG+RK+Y+KLF     E+++K             
Sbjct: 76  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKVSQCYLSTTAGPL 135

Query: 105 ------------------------NKGE----DQLVVIPKENVMRVNESENVKKASEKYI 146
                                   N G+       VVIPKE VMRVNESENVKKASEKYI
Sbjct: 136 AGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNESENVKKASEKYI 195

[Close]

BLAST of Lsi04G007820 vs. NCBI nr
Match: XP_038893105.1 (GEM-like protein 4 isoform X2 [Benincasa hispida])

HSP 1 Score: 178.3 bits (451), Expect = 4.9e-41
Identity = 116/224 (51.79%), Postives = 131/224 (58.48%), Query Frame = 0


Query: 1   MDSSFQAEQMVIGAPFRLPAALGRLRHDSVA--KLMMEEYNG--FKSMK----------- 60
           M SSFQ +QMVIGAPFRL AALGRLRHDSVA     M+ YNG  + S++           
Sbjct: 1   MASSFQDKQMVIGAPFRLTAALGRLRHDSVAASSKSMKRYNGKDYCSVRNRVSKMRRKAN 60

Query: 61  --------QLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLFEEVMKNKGEDQL---- 120
                    LRLGPK+ ETVK KLRLG RIVK+GGLRKV+EKLF EV  NKGE+ +    
Sbjct: 61  GFAHGIRDHLRLGPKIRETVKEKLRLGARIVKVGGLRKVHEKLFREVKNNKGEELVKASQ 120

Query: 121 ---------------------------------------------VVIPKENVMRVNESE 153
                                                        VVI KE VMRVNESE
Sbjct: 121 CYLSTTAGPIAGLLFISTHHIAFCSDTPIKISSPNGRHHFKFHYKVVIRKEKVMRVNESE 180

[Close]

BLAST of Lsi04G007820 vs. NCBI nr
Match: XP_038893104.1 (GEM-like protein 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 174.9 bits (442), Expect = 5.4e-40
Identity = 115/230 (50.00%), Postives = 127/230 (55.22%), Query Frame = 0


Query: 1   MDSSFQAEQMVIGAPFRLPAALGRLRHDSVA---------------------------KL 60
           M SSFQ +QMVIGAPFRL AALGRLRHDSVA                             
Sbjct: 1   MASSFQDKQMVIGAPFRLTAALGRLRHDSVAASSKSMKRCLMKIADNGKDYCSVRNRVSK 60

Query: 61  MMEEYNGFKS--MKQLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLFEEVMKNKGED 120
           M  + NGF       LRLGPK+ ETVK KLRLG RIVK+GGLRKV+EKLF EV  NKGE+
Sbjct: 61  MRRKANGFAHGIRDHLRLGPKIRETVKEKLRLGARIVKVGGLRKVHEKLFREVKNNKGEE 120

Query: 121 QL-------------------------------------------------VVIPKENVM 153
            +                                                 VVI KE VM
Sbjct: 121 LVKASQCYLSTTAGPIAGLLFISTHHIAFCSDTPIKISSPNGRHHFKFHYKVVIRKEKVM 180

[Close]

BLAST of Lsi04G007820 vs. NCBI nr
Match: XP_022139170.1 (GEM-like protein 4 [Momordica charantia])

HSP 1 Score: 128.6 bits (322), Expect = 4.4e-26
Identity = 95/220 (43.18%), Postives = 113/220 (51.36%), Query Frame = 0


Query: 1   MDSSFQAEQMVIGAPFRLPAALGRLR---HDSVAKLMMEEYNGFKSMKQ----------- 60
           M SS+Q   M  G PFR  AAL R R     S AKLM++ + G KS+KQ           
Sbjct: 1   MSSSYQEHMM--GVPFRSLAALTRRRLLPDHSAAKLMVKGHVGSKSLKQYNGKDHSLRNR 60

Query: 61  ------------------LRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EE 120
                             +RLGPK+TETVKGKL LG RIVK+GGLRKVYE +F     E+
Sbjct: 61  VNKLGRKADSFAHGFREHMRLGPKITETVKGKLSLGARIVKVGGLRKVYENMFRVSQGEK 120

Query: 121 VMK-----------------------------------NKGEDQL-----VVIPKENVMR 144
           ++K                                   +   D +     VVIP   VMR
Sbjct: 121 LLKASHCYLSTTAGPIAGLLFISTHKIAFCSDRSLKFSSPSGDHIRFHYKVVIPVGKVMR 180

[Close]

BLAST of Lsi04G007820 vs. NCBI nr
Match: KAG6601070.1 (GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 120.6 bits (301), Expect = 1.2e-23
Identity = 75/160 (46.88%), Postives = 94/160 (58.75%), Query Frame = 0


Query: 30  VAKLMMEEYNGFKSMKQLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EEV 89
           V +LM+  +  F+    ++LG K+TETVKGKL LG RIV++GG+RK+Y+KLF     E++
Sbjct: 84  VTRLMVHHFLSFEVGFAVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKL 143

Query: 90  MK------------------------------------NKGEDQL-----VVIPKENVMR 144
           +K                                      GE+ +     VVIP E VMR
Sbjct: 144 LKASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMR 203

[Close]

BLAST of Lsi04G007820 vs. NCBI nr
Match: XP_038893152.1 (GEM-like protein 4 [Benincasa hispida])

HSP 1 Score: 118.2 bits (295), Expect = 6.0e-23
Identity = 75/146 (51.37%), Postives = 93/146 (63.70%), Query Frame = 0


Query: 48  RLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLF-----EEVMKN--------------- 107
           +LG K++ETVKGKL LG RI+++GGLRK+Y+KLF     E+V+K                
Sbjct: 75  KLGAKISETVKGKLSLGGRILRVGGLRKMYKKLFSMSEGEKVLKASQCYLSTTNGPLAGL 134

Query: 108 ----------------KGEDQL-----VVIPKENVMRVNESENVKKASEKYIQIVTMDNF 153
                           K +D +     VVIPKE VMRVNESENVKKASEKYIQIVTMDNF
Sbjct: 135 LFISTHNIAFCSDKSIKLDDYVTFHYKVVIPKEKVMRVNESENVKKASEKYIQIVTMDNF 194

[Close]

BLAST of Lsi04G007820 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 92.0 bits (227), Expect = 4.3e-19
Identity = 59/179 (32.96%), Postives = 91/179 (50.84%), Query Frame = 0


Query: 19  PAALGRLRHDSVAKLMMEEYNGFKSM-----KQLRLGPKMTETVKGKLRLGLRIVKMGGL 78
           PA++ +L+  + +K+ M +     S       Q +LGPK+TETVK KL LG +I++MGGL
Sbjct: 27  PASINKLQIPTSSKVSMLQKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGL 86

Query: 79  RKVYEKLFEEVMKNK------------------------------GEDQL---------- 138
            K+Y++LF+   K K                               E  +          
Sbjct: 87  EKIYKRLFKVCDKEKLFKAYQCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLT 146

Query: 139 -----VVIPKENVMRVNESENVKKASEKYIQIVTMDNFEFWFMGFLNYQSTFKCLNKGV 148
                V IP   +  VN+S+N KK S++Y+++VT+DN++FWFMGF++YQ  F CL K +
Sbjct: 147 RVHYKVSIPLCKINGVNQSQNTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKAL 205

[Close]

BLAST of Lsi04G007820 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 91.3 bits (225), Expect = 7.3e-19
Identity = 59/180 (32.78%), Postives = 93/180 (51.67%), Query Frame = 0


Query: 16  FRLPAALGRLRHDSVAKLM-MEEYNGFKS--MKQLRLGPKMTETVKGKLRLGLRIVKMGG 75
           FR+PA+  +    +V  ++  ++ +GF +    Q ++ PK+TETVK KL LG RI+++GG
Sbjct: 33  FRVPASSKKSEQSNVKSILKRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGG 92

Query: 76  LRKVYEKLFE-----------------------------------------EVMKNKGE- 135
           L K++++LF                                          +V   +G+ 
Sbjct: 93  LEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDI 152

Query: 136 ---DQLVVIPKENVMRVNESENVKKASEKYIQIVTMDNFEFWFMGFLNYQSTFKCLNKGV 148
                 V IP   + RVN+S+N KK S+KY+++VT+D F+FWFMGFL+YQ  F CL K +
Sbjct: 153 IRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKAL 212

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BLAST of Lsi04G007820 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 90.9 bits (224), Expect = 9.6e-19
Identity = 55/147 (37.41%), Postives = 77/147 (52.38%), Query Frame = 0


Query: 46  QLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLFEEVMKNK----------------- 105
           Q +LGPK+TETVK KL LG +I++MGGL K+Y++LF+   + K                 
Sbjct: 129 QDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIA 188

Query: 106 -------------GEDQL---------------VVIPKENVMRVNESENVKKASEKYIQI 148
                         E  +               V IP   +  VN+S+N KK S+KY++I
Sbjct: 189 GLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKKPSQKYLEI 248

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BLAST of Lsi04G007820 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 88.6 bits (218), Expect = 4.8e-18
Identity = 54/147 (36.73%), Postives = 76/147 (51.70%), Query Frame = 0


Query: 46  QLRLGPKMTETVKGKLRLGLRIVKMGGLRKVYEKLFE----------------------- 105
           Q +LGPK+TETVK KL LG RI++MGGL K+Y++LF+                       
Sbjct: 67  QDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIA 126

Query: 106 ------------------EVMKNKGE----DQLVVIPKENVMRVNESENVKKASEKYIQI 148
                             +V   +GE       V IP   +  VN+S+N  K S+KY+++
Sbjct: 127 GLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYLEV 186

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BLAST of Lsi04G007820 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 64.3 bits (155), Expect = 9.6e-11
Identity = 27/58 (46.55%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 94  VVIPKENVMRVNESENVKKASEKYIQIVTMDNFEFWFMGFLNYQSTFKCLNKGVIDRK 152
           VV+  E +  ++ S NV K SE+Y+ +VT D FEFWFMGF++Y   F CLNK +++ +
Sbjct: 171 VVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAFNCLNKALLNSR 228

The following BLAST results are available for this feature:

BLAST of Lsi04G007820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMW5	6.1e-18	32.96	GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1	[more]
Q9FTA0	1.0e-17	32.78	GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1	[more]
Q9FMW6	1.3e-17	37.41	GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2	[more]
Q9FMW4	6.7e-17	36.73	Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV...	[more]
Q9M122	1.4e-09	46.55	GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1	[more]

BLAST of Lsi04G007820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CBK1	2.2e-26	43.18	GEM-like protein 4 OS=Momordica charantia OX=3673 GN=LOC111010140 PE=3 SV=1	[more]
A0A6J1KFK0	1.4e-22	48.30	putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493841 PE=3 SV=...	[more]
A0A6J1KB26	1.4e-22	48.30	putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493848 PE=3 SV=...	[more]
A0A5A7SSL2	1.4e-22	48.30	GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00...	[more]
A0A1S3CNX8	1.4e-22	48.30	GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1	[more]

BLAST of Lsi04G007820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893105.1	4.9e-41	51.79	GEM-like protein 4 isoform X2 [Benincasa hispida]	[more]
XP_038893104.1	5.4e-40	50.00	GEM-like protein 4 isoform X1 [Benincasa hispida]	[more]
XP_022139170.1	4.4e-26	43.18	GEM-like protein 4 [Momordica charantia]	[more]
KAG6601070.1	1.2e-23	46.88	GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038893152.1	6.0e-23	51.37	GEM-like protein 4 [Benincasa hispida]	[more]

BLAST of Lsi04G007820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23360.1	4.3e-19	32.96	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G08350.1	7.3e-19	32.78	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	9.6e-19	37.41	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	4.8e-18	36.73	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT4G01600.1	9.6e-11	46.55	GRAM domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 91..144 e-value: 3.4E-5 score: 23.7
IPR011993	PH-like domain superfamily	GENE3D	2.30.29.30		coord: 76..148 e-value: 5.0E-8 score: 34.7
None	No IPR available	PANTHER	PTHR31969:SF38	GEM-LIKE PROTEIN 4	coord: 45..82 coord: 94..147
IPR037848	GEM-like protein	PANTHER	PTHR31969	GEM-LIKE PROTEIN 2	coord: 45..82 coord: 94..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G007820.1	Lsi04G007820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity