Lsi04G007780 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G007780
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NEDD8-specific protease 1-like
Location	chr04: 7310799 .. 7311482 (-)
RNA-Seq Expression	Lsi04G007780
Synteny	Lsi04G007780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCTAAGTTCTTGACTTATGAAGATATCGTGCTTCGAAAATCCGATGTGCAAAGTCTTGAAGGTTGTAACTATTTAAACGACACTGTAATCGATTTCTATTTCGCCCATCTTTTTAGGTGCTATCAATCTAATGAAATTCTCTTGATTCCAACGAGCATATCATTTTTGCTAGCCAATGCTTTTGATTGGGATATTTTCATAGCCACAATCGAATCCCTAGACATTCATAAGAAGAAACTCATCTTCTTCACCATCAACGACAACTCCAATTTCGAATCTATTGACGGCGGATCTCACTGGAGCCTTCTCGTCTACTGCAGAACACGAAATTTGTTTATGCATTACGATAGCTTGAATCATATAAATTACGAATCGGCTTTCTCGCTTTATGAACCTTTAAATGGATATATAAGTCATGATTCGGCCAAGTTCATGGAGTGTGAGGTTCCTCAACAGAGGAATGGGTACGACTGCGGACTATACGTGATGGAAATTTCTAGGACAATCTGCGAATGCTATTTCAAGGTTGATCGATTTCTAAGCAAAGAAGATGATGATTTTTGTGGATTAATTAACGTGATGCGAAGTGTGAATTGTAATTATGTTGAGTCTGAGATGCGTTCTGAATTGCTACAGTTGATTTTGAGATTGAAGGACAAAGAAAACAAGTCTCAATAG

mRNA sequence

ATGGATCCTAAGTTCTTGACTTATGAAGATATCGTGCTTCGAAAATCCGATGTGCAAAGTCTTGAAGGTTGTAACTATTTAAACGACACTGTAATCGATTTCTATTTCGCCCATCTTTTTAGGTGCTATCAATCTAATGAAATTCTCTTGATTCCAACGAGCATATCATTTTTGCTAGCCAATGCTTTTGATTGGGATATTTTCATAGCCACAATCGAATCCCTAGACATTCATAAGAAGAAACTCATCTTCTTCACCATCAACGACAACTCCAATTTCGAATCTATTGACGGCGGATCTCACTGGAGCCTTCTCGTCTACTGCAGAACACGAAATTTGTTTATGCATTACGATAGCTTGAATCATATAAATTACGAATCGGCTTTCTCGCTTTATGAACCTTTAAATGGATATATAAGTCATGATTCGGCCAAGTTCATGGAGTGTGAGGTTCCTCAACAGAGGAATGGGTACGACTGCGGACTATACGTGATGGAAATTTCTAGGACAATCTGCGAATGCTATTTCAAGGTTGATCGATTTCTAAGCAAAGAAGATGATGATTTTTGTGGATTAATTAACGTGATGCGAAGTGTGAATTGTAATTATGTTGAGTCTGAGATGCGTTCTGAATTGCTACAGTTGATTTTGAGATTGAAGGACAAAGAAAACAAGTCTCAATAG

Coding sequence (CDS)

ATGGATCCTAAGTTCTTGACTTATGAAGATATCGTGCTTCGAAAATCCGATGTGCAAAGTCTTGAAGGTTGTAACTATTTAAACGACACTGTAATCGATTTCTATTTCGCCCATCTTTTTAGGTGCTATCAATCTAATGAAATTCTCTTGATTCCAACGAGCATATCATTTTTGCTAGCCAATGCTTTTGATTGGGATATTTTCATAGCCACAATCGAATCCCTAGACATTCATAAGAAGAAACTCATCTTCTTCACCATCAACGACAACTCCAATTTCGAATCTATTGACGGCGGATCTCACTGGAGCCTTCTCGTCTACTGCAGAACACGAAATTTGTTTATGCATTACGATAGCTTGAATCATATAAATTACGAATCGGCTTTCTCGCTTTATGAACCTTTAAATGGATATATAAGTCATGATTCGGCCAAGTTCATGGAGTGTGAGGTTCCTCAACAGAGGAATGGGTACGACTGCGGACTATACGTGATGGAAATTTCTAGGACAATCTGCGAATGCTATTTCAAGGTTGATCGATTTCTAAGCAAAGAAGATGATGATTTTTGTGGATTAATTAACGTGATGCGAAGTGTGAATTGTAATTATGTTGAGTCTGAGATGCGTTCTGAATTGCTACAGTTGATTTTGAGATTGAAGGACAAAGAAAACAAGTCTCAATAG

Protein sequence

MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLANAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSLNHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDRFLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRLKDKENKSQ

Homology

[Close]

BLAST of Lsi04G007780 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.0e-37
Identity = 86/219 (39.27%), Postives = 135/219 (61.64%), Query Frame = 0


Query: 2   DPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLAN 61
           D K L+YED+VLR+SD+  L G  +LND VI+FY + L   + S  I LIP SI+F ++N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  AFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSLN 121
             D +     ++ L++  K L+   +NDNSN E  +GG HWSLLVY +  N F+H+DS  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 HINYESAFSLYEPLNGYISHDSAKFMEC-EVPQQRNGYDCGLYVMEISRTICECYFKVDR 181
            +N  SA  L++ ++ ++S+  A + EC + PQQ+NGYDCG++++  +R ICE +F    
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRL 220
             ++++  F    NV  +V    + + +R E+L LI +L
Sbjct: 187 MKNRDELWFA---NVKETV--PDLVNHLREEILALIKKL 219

[Close]

BLAST of Lsi04G007780 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-25
Identity = 68/183 (37.16%), Postives = 104/183 (56.83%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDF---YFAH-LFRCYQSNEILLIPTSIS 60
           MDP  L+Y D +LR+SDV  L+  ++LND +I F   YFA+  F     +   + P    
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQ 60

Query: 61  FLLANAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMH 120
           F+   +   +I +  +E LD+  K+++F  INDNSN     GG+HWSLLVY + +N F H
Sbjct: 61  FIKCTSNPAEIAM-FLEPLDLPNKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFH 120

Query: 121 YDSLNHINYESAFSLYEPLNGYISH--DSAKFMECEVPQQRNGYDCGLYVMEISRTICEC 178
           YDS +  N   A  + E L  ++    D   F+E + P Q+N YDCG+YV+  +  +C+ 
Sbjct: 121 YDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQN 180

[Close]

BLAST of Lsi04G007780 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.8e-25
Identity = 67/183 (36.61%), Postives = 101/183 (55.19%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHL----FRCYQSNEILLIPTSIS 60
           MDP  L+Y D +LR+SDV  L+  N+LND +I F F +     F     +   + P    
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQ 60

Query: 61  FLLANAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMH 120
           F+   +   +I +  +E LD+  K+++F  INDNSN     GG+HWSLLVY + +N F H
Sbjct: 61  FIKCTSSPAEIAM-FLEPLDLPHKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFH 120

Query: 121 YDSLNHINYESAFSLYEPLNGYISH--DSAKFMECEVPQQRNGYDCGLYVMEISRTICEC 178
           YDS +  N   A  + E L  ++    D   F+E + P Q+N YDCG+YV+  +  +C+ 
Sbjct: 121 YDSHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQQNSYDCGMYVICNTEALCQN 180

[Close]

BLAST of Lsi04G007780 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 68/183 (37.16%), Postives = 102/183 (55.74%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDF---YFAH-LFRCYQSNEILLIPTSIS 60
           MDP  L+Y D +LR+SDV  L+  ++LND +I F   YFA+  F     +   + P    
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQ 60

Query: 61  FLLANAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMH 120
           F+   +   +I +  +E LD+  K+++F  INDNSN     GG+HWSLLVY + +N F H
Sbjct: 61  FIKCTSSPAEIAM-FLEPLDLPHKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFH 120

Query: 121 YDSLNHINYESAFSLYEPLNGYISH--DSAKFMECEVPQQRNGYDCGLYVMEISRTICEC 178
           YDS +  N   A  + E L  ++    D   F+E + P Q N YDCG+YV+  +  +C+ 
Sbjct: 121 YDSHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQENSYDCGMYVICNTEALCQS 180

[Close]

BLAST of Lsi04G007780 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.2e-22
Identity = 69/242 (28.51%), Postives = 123/242 (50.83%), Query Frame = 0


Query: 2   DPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQS------NEILLIPTSI 61
           DP  ++Y D  + +SD+  L+   +LND++I FY   L    +       N++LL+  S+
Sbjct: 3   DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSV 62

Query: 62  SFLLANA-FDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLF 121
            F  +    + +I +   + L +  K++IFF + +N +   I GG+HWSLL++ ++ N F
Sbjct: 63  VFCCSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNKF 122

Query: 122 MHYDSLNHINYESAFSLYEPLNGYISHDSAK------FMECEVPQQRNGYDCGLYVMEIS 181
           ++YDS+N  N   A  +       +S    K       +  + PQQ+NGYDCGLYV+ I 
Sbjct: 123 IYYDSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVLSII 182

Query: 182 RTICECYFKVDRFLSKEDD----------DFCGLINVMRSVNCNYVESEMRSELLQLILR 221
             + +   K +     E++          +F   + + + +  NY++ + R E+L  IL+
Sbjct: 183 EELLKLIIKENENNKGEENKISYKDLLLSEFTNEL-LFKEITPNYIK-DKRVEILNTILK 242

[Close]

BLAST of Lsi04G007780 vs. ExPASy TrEMBL
Match: A0A6J1JKF6 (NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.8e-85
Identity = 155/219 (70.78%), Postives = 184/219 (84.02%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           MDPKFLTY  +VLR+SDV+SLE CNYLND +IDFYFA+LF  YQS++ILL+PTSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           NA DWD FIAT+ESL+ H+KKLIFFT+NDNSNF S  GGSHWSLLVYCR RNLFMHYDSL
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSS-SGGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N ESAF++YE +N YIS+DSA+FMEC  P+QRNGYDCGLYVMEI+R I E YF  D+
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRL 220
           F  + +D+ CG  NV++ VNC +VES MRSE+LQLIL L
Sbjct: 181 F--QNEDEICGSFNVVQRVNCKHVESNMRSEMLQLILGL 216

[Close]

BLAST of Lsi04G007780 vs. ExPASy TrEMBL
Match: A0A6J1FTN2 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 9.2e-82
Identity = 148/215 (68.84%), Postives = 178/215 (82.79%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           MDPKFLTY  +VLR+SDV+SLE CNYLND +IDFYFA+LF  YQS++ILLI TSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           NA DWD F+AT+ESL+ H+KKLIFFT+NDNSNF S  GGSHWSLLVYCR RNLFMHYDSL
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSS-SGGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N ESAF++YE +N YIS+DSA+FMEC  P+QRN YDCGLYVMEI+R I E YF  D+
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQL 216
           F  + +D+ CG  NV++SVNC +VES MRSE + +
Sbjct: 181 F--QNEDEICGSFNVVQSVNCKHVESNMRSESINI 212

[Close]

BLAST of Lsi04G007780 vs. ExPASy TrEMBL
Match: A0A6J1GPM3 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 4.3e-79
Identity = 150/225 (66.67%), Postives = 179/225 (79.56%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           M  KFLTY D+V++  D+ SLEG NYLNDT+IDFYFA+L   YQSNEIL IPTS+SFLL 
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           N  DWDIFI+TIESL++H+KKLIFF +NDNSN  S  GGSHWSLLVYCR RNLFMHYDSL
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSS-SGGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N E AF+LYE ++GYI++D A+FMEC  P+Q NGYDCGLYVME +R I E YF VD+
Sbjct: 121 STMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRLKDKENK 226
           F SK  D+  G INVMRSV   YVES MRSE++QLI RLK ++ +
Sbjct: 181 FRSK--DEISGPINVMRSVKSKYVESNMRSEMMQLIRRLKQEQEQ 222

[Close]

BLAST of Lsi04G007780 vs. ExPASy TrEMBL
Match: A0A059ABJ7 (ULP_PROTEASE domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_J00959 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 3.5e-41
Identity = 103/229 (44.98%), Postives = 145/229 (63.32%), Query Frame = 0


Query: 2   DPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLAN 61
           D K L+Y+D+VLR+SD++ L G  +LND +I+FYF++L  CY S +ILL+P SI+F +AN
Sbjct: 7   DEKILSYKDVVLRRSDLEILSGPCFLNDRIIEFYFSYLSSCYPSPDILLVPPSIAFWIAN 66

Query: 62  AFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSLN 121
             D +     IE LD+  KKL+ F +NDN N    DGGSHWSLLV+ R  ++F+H+DS  
Sbjct: 67  CPDVEGLKDFIEPLDLPGKKLVIFPVNDNENVGLADGGSHWSLLVFERDASVFVHHDSSG 126

Query: 122 HINYESAFSLYEPLNGYI----SHDSAKFMEC-EVPQQRNGYDCGLYVMEISRTICECYF 181
            +N   A  LYE L G+I    S   AKF++C   PQQ NGYDCGLYV+  +R IC C++
Sbjct: 127 GMNGRYARQLYEVLVGFINGPNSASDAKFIQCSSSPQQLNGYDCGLYVIATARAIC-CWY 186

Query: 182 KVDRFLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRLKDKENK 226
           +  +  +++D  F    +V   V    V +E+R E+LQLI  L   + K
Sbjct: 187 ETVKLSNEKDLWFS---SVEEQVTPPSV-TELRKEILQLIKDLMTADAK 230

[Close]

BLAST of Lsi04G007780 vs. ExPASy TrEMBL
Match: A0A6P5ZU55 (NEDD8-specific protease 1-like OS=Durio zibethinus OX=66656 GN=LOC111303865 PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 3.9e-40
Identity = 98/228 (42.98%), Postives = 140/228 (61.40%), Query Frame = 0


Query: 2   DPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLAN 61
           D K L+Y D+VLR+SD++ L G  YLND +++FYF+ L   + S++ILL+P SISF + N
Sbjct: 7   DEKILSYNDVVLRRSDLEILSGRYYLNDRIVEFYFSLLSSSHPSHDILLVPPSISFWITN 66

Query: 62  AFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSLN 121
             D       +E L +  KKL+ F++N+N +  + +GGSHWSLL YCR+ N+F+H+DS  
Sbjct: 67  CPDVGDLKDFLEPLKLPDKKLVIFSVNNNDDVSAAEGGSHWSLLAYCRSANVFVHHDSNG 126

Query: 122 HINYESAFSLYEPLNGYISHDS----AKFMEC-EVPQQRNGYDCGLYVMEISRTICECYF 181
            +N   A  L++ L GYI   S    AK++EC   PQQ N YDCGLYV   +RTIC C+ 
Sbjct: 127 QMNKRHAMRLFKSLVGYIGSSSSDCNAKYLECIGTPQQVNSYDCGLYVTATARTIC-CW- 186

Query: 182 KVDRFLSKEDDDFCGL-INVMRSVNCNYVESEMRSELLQLILRLKDKE 224
               ++S E+ D   L  + ++      V SEMR E+L LI  L  K+
Sbjct: 187 ----YVSSENKDATDLWFSAVKEQVTPSVVSEMRKEILGLIKDLMAKK 228

[Close]

BLAST of Lsi04G007780 vs. NCBI nr
Match: XP_022988745.1 (NEDD8-specific protease 1-like [Cucurbita maxima])

HSP 1 Score: 325.5 bits (833), Expect = 3.7e-85
Identity = 155/219 (70.78%), Postives = 184/219 (84.02%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           MDPKFLTY  +VLR+SDV+SLE CNYLND +IDFYFA+LF  YQS++ILL+PTSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           NA DWD FIAT+ESL+ H+KKLIFFT+NDNSNF S  GGSHWSLLVYCR RNLFMHYDSL
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSS-SGGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N ESAF++YE +N YIS+DSA+FMEC  P+QRNGYDCGLYVMEI+R I E YF  D+
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRL 220
           F  + +D+ CG  NV++ VNC +VES MRSE+LQLIL L
Sbjct: 181 F--QNEDEICGSFNVVQRVNCKHVESNMRSEMLQLILGL 216

[Close]

BLAST of Lsi04G007780 vs. NCBI nr
Match: XP_022942008.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 313.2 bits (801), Expect = 1.9e-81
Identity = 148/215 (68.84%), Postives = 178/215 (82.79%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           MDPKFLTY  +VLR+SDV+SLE CNYLND +IDFYFA+LF  YQS++ILLI TSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           NA DWD F+AT+ESL+ H+KKLIFFT+NDNSNF S  GGSHWSLLVYCR RNLFMHYDSL
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSS-SGGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N ESAF++YE +N YIS+DSA+FMEC  P+QRN YDCGLYVMEI+R I E YF  D+
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQL 216
           F  + +D+ CG  NV++SVNC +VES MRSE + +
Sbjct: 181 F--QNEDEICGSFNVVQSVNCKHVESNMRSESINI 212

[Close]

BLAST of Lsi04G007780 vs. NCBI nr
Match: KAG7014407.1 (NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 308.1 bits (788), Expect = 6.1e-80
Identity = 152/223 (68.16%), Postives = 179/223 (80.27%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           M  KFLTY D+V+R SD+ SLEG NYLNDT+IDFYFA+L   YQSNEIL IPTS+S+LL 
Sbjct: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           N  DWDIFI+TIESL++H+KKLIFF INDNSN  S  GGSHWSLLVYCR RNLFMHYDSL
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSS-SGGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N E AF+LYE ++GYI++D A+FMEC  P+Q NGYDCGLYVME +R I E YF VD+
Sbjct: 121 STMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRLKDKE 224
           F SK  D+  G INVMRSV   YVES MRSE++QLI RLK ++
Sbjct: 181 FRSK--DEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 220

[Close]

BLAST of Lsi04G007780 vs. NCBI nr
Match: XP_022953908.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 304.3 bits (778), Expect = 8.8e-79
Identity = 150/225 (66.67%), Postives = 179/225 (79.56%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           M  KFLTY D+V++  D+ SLEG NYLNDT+IDFYFA+L   YQSNEIL IPTS+SFLL 
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           N  DWDIFI+TIESL++H+KKLIFF +NDNSN  S  GGSHWSLLVYCR RNLFMHYDSL
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSS-SGGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N E AF+LYE ++GYI++D A+FMEC  P+Q NGYDCGLYVME +R I E YF VD+
Sbjct: 121 STMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRLKDKENK 226
           F SK  D+  G INVMRSV   YVES MRSE++QLI RLK ++ +
Sbjct: 181 FRSK--DEISGPINVMRSVKSKYVESNMRSEMMQLIRRLKQEQEQ 222

[Close]

BLAST of Lsi04G007780 vs. NCBI nr
Match: KAG7031003.1 (NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.8 bits (774), Expect = 2.6e-78
Identity = 144/209 (68.90%), Postives = 172/209 (82.30%), Query Frame = 0


Query: 1   MDPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLA 60
           MDPKFLTY  +VLR+SDV+SLE CNYLND +IDFYFA+LF  YQ ++ILLIPTSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLG 60

Query: 61  NAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSL 120
           NA DWD F+AT+ESL+ H+KKLIFFT+NDNSNF S  GGSHWSLLVYCR RNLFMHY SL
Sbjct: 61  NASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSS-SGGSHWSLLVYCRKRNLFMHYHSL 120

Query: 121 NHINYESAFSLYEPLNGYISHDSAKFMECEVPQQRNGYDCGLYVMEISRTICECYFKVDR 180
           + +N ESAF++YE +N YIS+DSA+FMEC  P+QRN YDCGLYVMEI+R I E YF  D+
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQ 180

Query: 181 FLSKEDDDFCGLINVMRSVNCNYVESEMR 210
           F  + +D+ CG  NV++SVN  +VES MR
Sbjct: 181 F--QNEDEICGSFNVVQSVNRKHVESNMR 206

[Close]

BLAST of Lsi04G007780 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 7.3e-39
Identity = 86/219 (39.27%), Postives = 135/219 (61.64%), Query Frame = 0


Query: 2   DPKFLTYEDIVLRKSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLAN 61
           D K L+YED+VLR+SD+  L G  +LND VI+FY + L   + S  I LIP SI+F ++N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  AFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNLFMHYDSLN 121
             D +     ++ L++  K L+   +NDNSN E  +GG HWSLLVY +  N F+H+DS  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 HINYESAFSLYEPLNGYISHDSAKFMEC-EVPQQRNGYDCGLYVMEISRTICECYFKVDR 181
            +N  SA  L++ ++ ++S+  A + EC + PQQ+NGYDCG++++  +R ICE +F    
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FLSKEDDDFCGLINVMRSVNCNYVESEMRSELLQLILRL 220
             ++++  F    NV  +V    + + +R E+L LI +L
Sbjct: 187 MKNRDELWFA---NVKETV--PDLVNHLREEILALIKKL 219

[Close]

BLAST of Lsi04G007780 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.1e-06
Identity = 48/194 (24.74%), Postives = 77/194 (39.69%), Query Frame = 0


Query: 15  KSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLANAFDWDIFIATI-- 74
           K DV+ L+   ++NDT+IDFY  +L    Q+ E        SF      D D   ++I  
Sbjct: 430 KRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIAD 489

Query: 75  ------------ESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNL------- 134
                         +D+  K  IF  +N N          HWSL+V C    +       
Sbjct: 490 GKAAFLRVRKWTRKVDMFGKDYIFVPVNYN---------LHWSLIVICHPGEVANRTDLD 549

Query: 135 ---------FMHYDSL--NHINYESAFSLY-----EPLNGYISHD------SAKFMECEV 166
                     +H DS+  +H   ++    Y     +  +   S D      + +F+  E+
Sbjct: 550 LDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLEL 609

[Close]

BLAST of Lsi04G007780 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.1e-06
Identity = 48/194 (24.74%), Postives = 77/194 (39.69%), Query Frame = 0


Query: 15  KSDVQSLEGCNYLNDTVIDFYFAHLFRCYQSNEILLIPTSISFLLANAFDWDIFIATI-- 74
           K DV+ L+   ++NDT+IDFY  +L    Q+ E        SF      D D   ++I  
Sbjct: 398 KRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIAD 457

Query: 75  ------------ESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNL------- 134
                         +D+  K  IF  +N N          HWSL+V C    +       
Sbjct: 458 GKAAFLRVRKWTRKVDMFGKDYIFVPVNYN---------LHWSLIVICHPGEVANRTDLD 517

Query: 135 ---------FMHYDSL--NHINYESAFSLY-----EPLNGYISHD------SAKFMECEV 166
                     +H DS+  +H   ++    Y     +  +   S D      + +F+  E+
Sbjct: 518 LDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLEL 577

[Close]

BLAST of Lsi04G007780 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 2.8e-06
Identity = 41/204 (20.10%), Postives = 80/204 (39.22%), Query Frame = 0


Query: 11  IVLRKSDVQSLEGCNYLNDTVIDFYFA--------------HLFRCYQSNEILLIPTSIS 70
           +V+RK D++ L+   ++NDT+IDFY                H F C+   ++  +     
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKLANLDKGTP 364

Query: 71  FLLANAFDWDIFIATIESLDIHKKKLIFFTINDNSNFESIDGGSHWSLLVYCRTRNL--- 130
                   +       +++D+ +K  IF  IN +          HWSL++ C    L   
Sbjct: 365 STCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS---------FHWSLVIICHPGELVPS 424

Query: 131 -------------------FMHYDSLNHINYESAFSLY--------EPLNGYISHDSAK- 166
                               +H DS+   +     +++        +  +   ++DS++ 
Sbjct: 425 HVNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRA 484

The following BLAST results are available for this feature:

BLAST of Lsi04G007780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSS7	1.0e-37	39.27	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
Q96LD8	4.5e-25	37.16	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
Q5FVJ8	5.8e-25	36.61	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
Q9D2Z4	1.7e-24	37.16	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
Q54XR2	1.2e-22	28.51	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Lsi04G007780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKF6	1.8e-85	70.78	NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 ...	[more]
A0A6J1FTN2	9.2e-82	68.84	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=...	[more]
A0A6J1GPM3	4.3e-79	66.67	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=...	[more]
A0A059ABJ7	3.5e-41	44.98	ULP_PROTEASE domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ...	[more]
A0A6P5ZU55	3.9e-40	42.98	NEDD8-specific protease 1-like OS=Durio zibethinus OX=66656 GN=LOC111303865 PE=3...	[more]

BLAST of Lsi04G007780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988745.1	3.7e-85	70.78	NEDD8-specific protease 1-like [Cucurbita maxima]	[more]
XP_022942008.1	1.9e-81	68.84	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
KAG7014407.1	6.1e-80	68.16	NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022953908.1	8.8e-79	66.67	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
KAG7031003.1	2.6e-78	68.90	NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lsi04G007780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60190.1	7.3e-39	39.27	Cysteine proteinases superfamily protein	[more]
AT1G09730.1	2.1e-06	24.74	Cysteine proteinases superfamily protein	[more]
AT1G09730.2	2.1e-06	24.74	Cysteine proteinases superfamily protein	[more]
AT4G33620.1	2.8e-06	20.10	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 27..171 e-value: 2.1E-12 score: 47.4
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 11..171 score: 16.723837
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 1..222 e-value: 1.3E-54 score: 187.2
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 2..221
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G007780.1	Lsi04G007780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0008234	cysteine-type peptidase activity