Lsi04G007690 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G007690
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPyruvate kinase family protein
Locationchr04: 7204967 .. 7209025 (-)
RNA-Seq ExpressionLsi04G007690
SyntenyLsi04G007690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGTATTTCGACAAATATTCTTTAATAATATGAATATATAATTTTACAGAGTGGTATATGAAAAGAAGCACAAACGGTGGTAGCCGAAGCTTTGAAGCACACAGAACTCAACACTACAAAACCCAAGAGATTTGAAGAAGAAGGAAGAAGGAAGAAGGAGAAAATCGTACAAGTTTGTAGTTGGAGCTATTAGCGCCTTCATCAGCAAAAGTATATCGTATATGAATCTTTCTATCGACCGCTTTTTTCTGTTTCTGTTCATCTTTTTCTTATACTCATCATAGATTTTGTTGTTTTGATGTTTCCTTGTTTTTCTCTTCCTAATTTTGTGTTTATATAGAGTCTTCGTGTTTTTTGGATCTCATTCATTTACCTGGATTTAATTTTTTTATGATTTTGATTTCATGCGTTTCACTTTTTAGTTTTTGGTTTGATTTCTGATTTGCTTAGGAAAAAATAGTTGCAGATGGCCAATATTGATATTGAGGGGATTCTTAAGGAACTTCCTAATGATGGGCGCATCCCCAAAACGAAAATTGTTTGTACTTTGGGACCTGCTTCCCGATCGGTGCCTATGATCGAGAAGCTTCTTAGGGCTGGTATGAATGTTGCTCGATTCAATTTCTCTCATGGTACACATGAGTATCATCAAGAGACTTTGAACAATCTCAGGACTGCCATGCAGAATACTCAAATCCTTTGCGCCGTCATGCTTGATACTAAGGTCTTTCTCATGCTCTGATCTTTGCTTGAAACTAATGGGTTAGGCAATAATGTGAGATGGTATTTTATTAAATTCCTTAAGTATTTATTTTTGGTTCTGGCTTGGGTGATCTGGAAATGAATAGAAGAAGGATCTGGTTAATGAACTTGCTTTCACCCATTTTGTTGCTTCTCAATTCACTTGCTAGGTTAAATACATTGCAAAAATGACACTTTGTTAAAATTGTTTTTCATATTTTTTTTCCTCTGTCAATACATCTAATGAAACCATCTAATTTGTAGGAAAACCACCACATGTAAATACCATGCTTGAAAACCTAACTGTTGCAAAGAAGTAAAATGTGGGTTCTATTTGAAATTTTGACAACCTGCTAAGCCAATATGTAGTTAACAAAAAATATTAAATCTAGAATTTCACATGAGAAATAGTTAGGTTCGCTACTATATTTTGATTGACAATTATGGATCTGAACAAGGTCTTTAGCTATCTTACAACTCCTTCATTATCCTTTCCGCATCAAATGTCTAATGTGAATATGATGTCTATTTAAGAGATTTTTCCTGTTGATGTACCATCTTTTTGCAGCTTTTCATTCACAATTCTAATTTCTAACTATAGGGCTTCTTAAATCTCTATGCTAGGCCACATATATAATTTTTTTTCTGGGTAAAGATGGAACTTGGGGTTTGAATATACACTTCTGATACTTATGAAAGAAAGTATATACTTACTAAAAAACAGGATCAGATTAACACTTATTATGAGAAAAGGTATTGAATATGCATTTCTTATTAAAAAAGGATCAAGTATACACTGCGTACACCAACTCATTCACTTTTGAAGAGTTCAACACATCTGGCATAATTGGTTCACTTAGTATCGTGTTTTTTACCACTTGCAAACTGATTTTTCATGAAGCCTCCTCTTGTTTACCATGGCAGGGACCGGAGATTCGGACTGGTTTCCTGAAGGATGGGAAACCAATTCAACTGAAAGAAGGCCAAGAAGTGATCATAACCACGGATTATAGCATCAAGGGGGATGAGGAAATGATCTCGATGAGTTATCAGAAGCTTGCTGTGGACTTGAAGCCAGGGAATACTATATTGTGTTCAGATGGCACAATCACCCTCACAGTTTTGTCTTGTGATTCAGAAGCTGGGAGGGTGATATGTCGTTGTGAAAACACTGCAATGTTGGGAGAAAGAAAGAATGTGAATCTTCCTGGTGTAGTGGTAGATCTTCCCACTCTGACAGAAAAGGATAAAGAGGATATTCTTGGTTGGGGTGTTCCCAATAGTATCGATATGATTGCTCTTTCATTTGTTCGTAAGGGTTCTGATCTAGTCAATGTCCGCAAGATTCTTGGCCCCCATGCCAAACATATAAAACTGATGTCAAAGGTATGTATTTTGCTTTCGGGTCCTGTTTTTATTATTGTTATTATTTTTATATTGAATGAATATTATTGTATTATTGTCTCTTAGCTTAAAAAAGCATTGTTATTGTTGTTTGGGGAGGGGTGTAGATTGAACAGTCCTGGATTTTACATTGCATATGTTGCCTGACTGCACATTTTTATCAGGGAAATCAGCATTTAATGCCTAAAGGCATAGTTTGAAGGTTAGCATTGTTTATTCGCGTATCCTCCTCTCTTTTAAAAAATTGTCAGTGCATTAGCTGCTGTTCGGCTGACCTTGCTTTGATGATTTATTCCTTTTGAAATTGTTGTTGTCATTGCTTCCATGTGTACATGGTGTGCACAACGTCTTGAATTCTTAGTCATTGGTGCGTACTTTATTTGCAGGATAGGGTTAGTAGGTTGAGGAATCACTCGCTATCATATTTTACTTTTCATGCATGCTATGTTTAAGTCTTTCTTCTTACATACTTTTCATTGAGTATGTTTCTTCAAAGATAATGTTCATTTTGACATTGAATGTCGTTATATCTGTTGGATCAAATAGTAATTTGTGTTTTAATGTGAATACCCAAGGTTGAGAACCAGGAGGGAGTTATCAATTTTGATGAAATTTTGCGTGAGACTGATGCATTCATGGTTGCTCGAGGTGATCTTGGAATGGAAATTCCAGTTGAGAAGATCTTCTTGGCACAAAAAATGATGATTTACAAGTGTAATCTTGCTGGCAAGCCTGTGGTTACAGCTACTCAGATGCTTGAATCAATGATCAAATCTCCTCGGCCAACTCGTGCAGAAGCCACAGATGTCGCTAATGCCGTTCTCGACGGCACCGATTGCGTTATGCTTAGTGGTGAGAGTGCAGCTGGGTCTTATCCTGAACTCGCTGTAAAGATCATGGCTCGTATTTGTATTGAAGCTGAATCATCCTTGGACTATGGGGTCATTTTTAAGGAGATGATAAGGTCCACCCCACTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCAGCCGTTCGAACCGCTAACAAGGCAAAAGCAAAACTCATCGTCGTGCTGACTCGTGGTGGAACCACAGCCAAGTTGGTTGCCAAGTATAGGCCAGCTGTTCCAATCCTCTCAGTTGTTGTTCCAGTTTTAACTACCGATTCTTTCGATTGGTCCTGCAGTGACGAGTCCCCAGCCAGACACAGCCTGATCCACAGAGGCTTGATTCCTATATTGGCTGAAGGATCTGCTAAGGCCACCGATGCTGAATCCACAGAAGTGATACTGGAAGCTGCTCTGAAATCTGCAATGGGGAAGGGACTGTGCAAACCTGGTGATGCCATCGTTGCACTGCACCGGATCGGAGCTGCCTCCGTCATCAAGATATGCATTGTGAAATGAATCAACTAAACAAGCAAATCTGGCATCGGTTCCTTCATCTAGAAGTTGCTCAGGTGCATTCTCTATCCTTTTGCTATTTATTATATAAATATTGTTATTTTTAGTATTTCACCTAAAGAGGGTTGAACCGGTAATTTTGAGTTTCTTGTTGTGAAAGAGGGGTGGGTATTCCCTTGATTTTTTCTTTTTGGACTGCCTGCTGTGTGACTTCGAAAAGTAGGGGAAGTAAAGAATTGAATCAACTTTCATAAGCTTTTGTTCTTGAAATTCCCAAAATTTTGTGCCCTAATTTGTGTTTTGTAGAACTCTAAATCCATTTGTACTCATGTGACATCTGAAACAGATCATTTGGATGGACTTTTATTATATGAGGTTTATTCCATAGTAGTTCAGTTTTCAATAATATATGCTTCCATTTATATTAAACTTGGAGCTCTTATTCATATGTAATTTTAATACATTTTTAAGGGGTTTCCCCCCTTAAAAACTATGGTGTCTAATCCAATAAATTTTATTATTACATTAA

mRNA sequence

TGGTATTTCGACAAATATTCTTTAATAATATGAATATATAATTTTACAGAGTGGTATATGAAAAGAAGCACAAACGGTGGTAGCCGAAGCTTTGAAGCACACAGAACTCAACACTACAAAACCCAAGAGATTTGAAGAAGAAGGAAGAAGGAAGAAGGAGAAAATCGTACAAGTTTGTAGTTGGAGCTATTAGCGCCTTCATCAGCAAAAGAAAAAATAGTTGCAGATGGCCAATATTGATATTGAGGGGATTCTTAAGGAACTTCCTAATGATGGGCGCATCCCCAAAACGAAAATTGTTTGTACTTTGGGACCTGCTTCCCGATCGGTGCCTATGATCGAGAAGCTTCTTAGGGCTGGTATGAATGTTGCTCGATTCAATTTCTCTCATGGTACACATGAGTATCATCAAGAGACTTTGAACAATCTCAGGACTGCCATGCAGAATACTCAAATCCTTTGCGCCGTCATGCTTGATACTAAGGGACCGGAGATTCGGACTGGTTTCCTGAAGGATGGGAAACCAATTCAACTGAAAGAAGGCCAAGAAGTGATCATAACCACGGATTATAGCATCAAGGGGGATGAGGAAATGATCTCGATGAGTTATCAGAAGCTTGCTGTGGACTTGAAGCCAGGGAATACTATATTGTGTTCAGATGGCACAATCACCCTCACAGTTTTGTCTTGTGATTCAGAAGCTGGGAGGGTGATATGTCGTTGTGAAAACACTGCAATGTTGGGAGAAAGAAAGAATGTGAATCTTCCTGGTGTAGTGGTAGATCTTCCCACTCTGACAGAAAAGGATAAAGAGGATATTCTTGGTTGGGGTGTTCCCAATAGTATCGATATGATTGCTCTTTCATTTGTTCGTAAGGGTTCTGATCTAGTCAATGTCCGCAAGATTCTTGGCCCCCATGCCAAACATATAAAACTGATGTCAAAGGTTGAGAACCAGGAGGGAGTTATCAATTTTGATGAAATTTTGCGTGAGACTGATGCATTCATGGTTGCTCGAGGTGATCTTGGAATGGAAATTCCAGTTGAGAAGATCTTCTTGGCACAAAAAATGATGATTTACAAGTGTAATCTTGCTGGCAAGCCTGTGGTTACAGCTACTCAGATGCTTGAATCAATGATCAAATCTCCTCGGCCAACTCGTGCAGAAGCCACAGATGTCGCTAATGCCGTTCTCGACGGCACCGATTGCGTTATGCTTAGTGGTGAGAGTGCAGCTGGGTCTTATCCTGAACTCGCTGTAAAGATCATGGCTCGTATTTGTATTGAAGCTGAATCATCCTTGGACTATGGGGTCATTTTTAAGGAGATGATAAGGTCCACCCCACTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCAGCCGTTCGAACCGCTAACAAGGCAAAAGCAAAACTCATCGTCGTGCTGACTCGTGGTGGAACCACAGCCAAGTTGGTTGCCAAGTATAGGCCAGCTGTTCCAATCCTCTCAGTTGTTGTTCCAGTTTTAACTACCGATTCTTTCGATTGGTCCTGCAGTGACGAGTCCCCAGCCAGACACAGCCTGATCCACAGAGGCTTGATTCCTATATTGGCTGAAGGATCTGCTAAGGCCACCGATGCTGAATCCACAGAAGTGATACTGGAAGCTGCTCTGAAATCTGCAATGGGGAAGGGACTGTGCAAACCTGGTGATGCCATCGTTGCACTGCACCGGATCGGAGCTGCCTCCGTCATCAAGATATGCATTGTGAAATGAATCAACTAAACAAGCAAATCTGGCATCGGTTCCTTCATCTAGAAGTTGCTCAGGTGCATTCTCTATCCTTTTGCTATTTATTATATAAATATTGTTATTTTTAGTATTTCACCTAAAGAGGGTTGAACCGGTAATTTTGAGTTTCTTGTTGTGAAAGAGGGGTGGGTATTCCCTTGATTTTTTCTTTTTGGACTGCCTGCTGTGTGACTTCGAAAAGTAGGGGAAGTAAAGAATTGAATCAACTTTCATAAGCTTTTGTTCTTGAAATTCCCAAAATTTTGTGCCCTAATTTGTGTTTTGTAGAACTCTAAATCCATTTGTACTCATGTGACATCTGAAACAGATCATTTGGATGGACTTTTATTATATGAGGTTTATTCCATAGTAGTTCAGTTTTCAATAATATATGCTTCCATTTATATTAAACTTGGAGCTCTTATTCATATGTAATTTTAATACATTTTTAAGGGGTTTCCCCCCTTAAAAACTATGGTGTCTAATCCAATAAATTTTATTATTACATTAA

Coding sequence (CDS)

ATGGCCAATATTGATATTGAGGGGATTCTTAAGGAACTTCCTAATGATGGGCGCATCCCCAAAACGAAAATTGTTTGTACTTTGGGACCTGCTTCCCGATCGGTGCCTATGATCGAGAAGCTTCTTAGGGCTGGTATGAATGTTGCTCGATTCAATTTCTCTCATGGTACACATGAGTATCATCAAGAGACTTTGAACAATCTCAGGACTGCCATGCAGAATACTCAAATCCTTTGCGCCGTCATGCTTGATACTAAGGGACCGGAGATTCGGACTGGTTTCCTGAAGGATGGGAAACCAATTCAACTGAAAGAAGGCCAAGAAGTGATCATAACCACGGATTATAGCATCAAGGGGGATGAGGAAATGATCTCGATGAGTTATCAGAAGCTTGCTGTGGACTTGAAGCCAGGGAATACTATATTGTGTTCAGATGGCACAATCACCCTCACAGTTTTGTCTTGTGATTCAGAAGCTGGGAGGGTGATATGTCGTTGTGAAAACACTGCAATGTTGGGAGAAAGAAAGAATGTGAATCTTCCTGGTGTAGTGGTAGATCTTCCCACTCTGACAGAAAAGGATAAAGAGGATATTCTTGGTTGGGGTGTTCCCAATAGTATCGATATGATTGCTCTTTCATTTGTTCGTAAGGGTTCTGATCTAGTCAATGTCCGCAAGATTCTTGGCCCCCATGCCAAACATATAAAACTGATGTCAAAGGTTGAGAACCAGGAGGGAGTTATCAATTTTGATGAAATTTTGCGTGAGACTGATGCATTCATGGTTGCTCGAGGTGATCTTGGAATGGAAATTCCAGTTGAGAAGATCTTCTTGGCACAAAAAATGATGATTTACAAGTGTAATCTTGCTGGCAAGCCTGTGGTTACAGCTACTCAGATGCTTGAATCAATGATCAAATCTCCTCGGCCAACTCGTGCAGAAGCCACAGATGTCGCTAATGCCGTTCTCGACGGCACCGATTGCGTTATGCTTAGTGGTGAGAGTGCAGCTGGGTCTTATCCTGAACTCGCTGTAAAGATCATGGCTCGTATTTGTATTGAAGCTGAATCATCCTTGGACTATGGGGTCATTTTTAAGGAGATGATAAGGTCCACCCCACTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCAGCCGTTCGAACCGCTAACAAGGCAAAAGCAAAACTCATCGTCGTGCTGACTCGTGGTGGAACCACAGCCAAGTTGGTTGCCAAGTATAGGCCAGCTGTTCCAATCCTCTCAGTTGTTGTTCCAGTTTTAACTACCGATTCTTTCGATTGGTCCTGCAGTGACGAGTCCCCAGCCAGACACAGCCTGATCCACAGAGGCTTGATTCCTATATTGGCTGAAGGATCTGCTAAGGCCACCGATGCTGAATCCACAGAAGTGATACTGGAAGCTGCTCTGAAATCTGCAATGGGGAAGGGACTGTGCAAACCTGGTGATGCCATCGTTGCACTGCACCGGATCGGAGCTGCCTCCGTCATCAAGATATGCATTGTGAAATGA

Protein sequence

MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNLPGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSKVENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCKPGDAIVALHRIGAASVIKICIVK
Homology
BLAST of Lsi04G007690 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 6.5e-266
Identity = 470/511 (91.98%), Postives = 488/511 (95.50%), Query Frame = 0

Query: 1   MANIDIEGILK-ELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHE 60
           MANIDIEGILK + P DGR+PKTKIVCTLGPASRSV M EKLLRAGMNVARFNFSHGTH+
Sbjct: 1   MANIDIEGILKQQQPYDGRVPKTKIVCTLGPASRSVEMTEKLLRAGMNVARFNFSHGTHD 60

Query: 61  YHQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKG 120
           YHQETLNNL+TAM NT ILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEV ITTDY IKG
Sbjct: 61  YHQETLNNLKTAMHNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVTITTDYDIKG 120

Query: 121 DEEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVN 180
           D EMISMSY+KL V LKPGNTILCSDGTITLTVLSCD +AG V CRCENTA LGERKNVN
Sbjct: 121 DPEMISMSYKKLPVHLKPGNTILCSDGTITLTVLSCDPDAGTVRCRCENTATLGERKNVN 180

Query: 181 LPGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMS 240
           LPGVVVDLPTLTEKDKEDILGWGVPN IDMIALSFVRKGSDLVNVRK+LGPHAK+I+LMS
Sbjct: 181 LPGVVVDLPTLTEKDKEDILGWGVPNKIDMIALSFVRKGSDLVNVRKVLGPHAKNIQLMS 240

Query: 241 KVENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQ 300
           KVENQEGV+NFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNL GKPVVTATQ
Sbjct: 241 KVENQEGVLNFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQ 300

Query: 301 MLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSL 360
           MLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAG+YPELAVKIMARICIEAESSL
Sbjct: 301 MLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPELAVKIMARICIEAESSL 360

Query: 361 DYGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVP 420
           DYG IFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGG+TAKLVAKYRPAVP
Sbjct: 361 DYGAIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGSTAKLVAKYRPAVP 420

Query: 421 ILSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKS 480
           ILSVVVPVL+TDSFDW+CSDE+PARHSLI+RGLIPIL EGSAKATDAESTEVILEAALKS
Sbjct: 421 ILSVVVPVLSTDSFDWTCSDETPARHSLIYRGLIPILGEGSAKATDAESTEVILEAALKS 480

Query: 481 AMGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           A  + LCKPGDA+VALHRIGAASVIKICIVK
Sbjct: 481 ATERALCKPGDAVVALHRIGAASVIKICIVK 511

BLAST of Lsi04G007690 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 1.7e-261
Identity = 455/510 (89.22%), Postives = 489/510 (95.88%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDI GI+K+LPNDGRIPKTKIVCTLGP+SR+VPM+EKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIAGIMKDLPNDGRIPKTKIVCTLGPSSRTVPMLEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETL+NL+ AMQNTQILCAVMLDTKGPEIRTGFL DGKPIQLKEGQE+ ++TDY+IKG+
Sbjct: 61  HQETLDNLKIAMQNTQILCAVMLDTKGPEIRTGFLTDGKPIQLKEGQEITVSTDYTIKGN 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSY+KL +DLKPGNTILC+DGTITLTVLSCD  +G V CRCENTA LGERKNVNL
Sbjct: 121 EEMISMSYKKLVMDLKPGNTILCADGTITLTVLSCDPPSGTVRCRCENTATLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLTEKDKEDIL WGVPN+IDMIALSFVRKGSDLVNVRK+LGPHAK I+LMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILEWGVPNNIDMIALSFVRKGSDLVNVRKVLGPHAKRIQLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETD+FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGK VVTATQM
Sbjct: 241 VENQEGVINFDEILRETDSFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKAVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSP PTRAEATDVANAVLDGTDCVMLSGESAAG+YPELAVKIM+RICIEAESSLD
Sbjct: 301 LESMIKSPAPTRAEATDVANAVLDGTDCVMLSGESAAGAYPELAVKIMSRICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
              IFKEMIR TPLPMSPLESLASSAVRTANKA+AKLIVVLTRGG+TAKLVAKYRPAVPI
Sbjct: 361 NEAIFKEMIRCTPLPMSPLESLASSAVRTANKARAKLIVVLTRGGSTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWS SDE+PARHSL++RGLIP+L EGSAKATD+ESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWSISDETPARHSLVYRGLIPLLGEGSAKATDSESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           + +GLCKPGDA+VALHRIG+ASVIKIC+VK
Sbjct: 481 VTRGLCKPGDAVVALHRIGSASVIKICVVK 510

BLAST of Lsi04G007690 vs. ExPASy Swiss-Prot
Match: Q42954 (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.0e-223
Identity = 389/491 (79.23%), Postives = 439/491 (89.41%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRTAMQNTQILC 79
           PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHG+H+YHQET++NLR AM++T ILC
Sbjct: 18  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGSHDYHQETIDNLRQAMESTGILC 77

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKD KP+QLK+GQE+ I+TDYSIKGDE MI MSY+KLA D+KP +
Sbjct: 78  AVMLDTKGPEIRTGFLKDAKPVQLKQGQEITISTDYSIKGDESMICMSYKKLAEDVKPQS 137

Query: 140 TILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNLPGVVVDLPTLTEKDKEDIL 199
            ILC+DG IT TVLSCD E G   CRCENTA+LGERKNVNLPGV+VDLPTLT+KDK+DIL
Sbjct: 138 VILCADGQITFTVLSCDKENGLDRCRCENTAVLGERKNVNLPGVIVDLPTLTDKDKDDIL 197

Query: 200 GWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSKVENQEGVINFDEILRETDA 259
            WGVPN IDMIALSFVRKGSDLV VRK+LG HAK+I LMSKVENQEGV NFD+IL  +DA
Sbjct: 198 NWGVPNHIDMIALSFVRKGSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDDILLNSDA 257

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 258 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 317

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRSTPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YP+LAV  MA+ICIEAES++DY  +FK ++ + P+PMSPL
Sbjct: 318 NAVLDGTDCVMLSGETAAGAYPDLAVGTMAKICIEAESTIDYPDVFKRIMSNAPVPMSPL 377

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWSCSD 439
           ESLASSAVRTAN AKA LI+VLTRGG+TAKLVAKYRP +PILSVVVP + TDSFDW+CSD
Sbjct: 378 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGMPILSVVVPEIKTDSFDWTCSD 437

Query: 440 ESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCKPGDAIVALHRIG 499
           ESPARHSLI RGL+P+L  GSA+A+  ESTE  L+ AL+ A  KGLCK GD++VALHR+G
Sbjct: 438 ESPARHSLIFRGLVPVLHAGSARASHEESTEEALDFALQHAKTKGLCKQGDSVVALHRVG 497

Query: 500 AASVIKICIVK 511
            ASVIKI  VK
Sbjct: 498 TASVIKIVTVK 508

BLAST of Lsi04G007690 vs. ExPASy Swiss-Prot
Match: O65595 (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g26390 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 8.0e-216
Identity = 378/491 (76.99%), Postives = 431/491 (87.78%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRTAMQNTQILC 79
           PKTKIVCTLGPASRSVPM+EKLL AGM+VARFNFSHG++EYHQETL+NLR AM NT +LC
Sbjct: 7   PKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLC 66

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKDGKPIQLK+GQE+ I+TDY +KGDE+ I MSY+KLA D+ PG 
Sbjct: 67  AVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPGM 126

Query: 140 TILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNLPGVVVDLPTLTEKDKEDIL 199
            ILC+DGTI+L VLSCD E G V CRCENT+MLGERKNVNLPGVVVDLPTLTEKDK+DIL
Sbjct: 127 VILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEKDKQDIL 186

Query: 200 GWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSKVENQEGVINFDEILRETDA 259
            WGVPN IDMIALSFVRKGSDLV VRK+LG HAK I LMSKVENQEGV NFD+IL  +DA
Sbjct: 187 EWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDILINSDA 246

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FM+ARGDLGMEIP+EKIFLAQK+MIYKCN  GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 247 FMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRAEATDVA 306

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRSTPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YPELAV+ MA+IC+EAES+LDYG IFK ++    +PMSP+
Sbjct: 307 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAAVPMSPM 366

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWSCSD 439
           ESLASSAVRTA  ++A L++VLTRGG+TA+LVAKYRP +PILSVVVP +T+DSFDW+CS+
Sbjct: 367 ESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSFDWACSN 426

Query: 440 ESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCKPGDAIVALHRIG 499
           E+PARHSLI+RGL+P+L  GSA+A+  ESTE  LE A +    K LCK GD++VAL R G
Sbjct: 427 EAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVVALFRTG 486

Query: 500 AASVIKICIVK 511
            A VIKI  VK
Sbjct: 487 NAIVIKILTVK 497

BLAST of Lsi04G007690 vs. ExPASy Swiss-Prot
Match: O44006 (Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.7e-125
Identity = 245/496 (49.40%), Postives = 334/496 (67.34%), Query Frame = 0

Query: 21  KTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRTAM-QNTQILC 80
           +TKIVCT+GP+   V  + +L+ AGMNV R NFSHG HE H   + NL+ A+ Q      
Sbjct: 57  RTKIVCTMGPSCWDVDKMVQLIDAGMNVCRLNFSHGDHEAHGRVVKNLQEALKQRPGKRV 116

Query: 81  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISMSYQKLAVDLKPGN 140
           A++LDTKGPEIRTG L+  KPI+L  G  + I TDYS  G++  I+ SY+KL   +KPGN
Sbjct: 117 ALLLDTKGPEIRTGMLEGDKPIELHAGDMLKIVTDYSFVGNKSCIACSYEKLPSSVKPGN 176

Query: 141 TILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNLPGVVVDLPTLTEKDKEDIL 200
           TIL +DG++++ V+ C  +   V+ R  N A++G +KN+NLPGV VDLP + EKDK DIL
Sbjct: 177 TILIADGSLSVEVVECGKD--YVMTRVMNPAIIGNKKNMNLPGVKVDLPVIGEKDKNDIL 236

Query: 201 GWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSKVENQEGVINFDEILRETDA 260
            +G+P   + IA SFV+   D+  +R ILG   ++IK++ K+EN EG++NFDEIL+E D 
Sbjct: 237 NFGIPMGCNFIAASFVQSADDVRYIRSILGTKGRNIKIIPKIENVEGLLNFDEILQEADG 296

Query: 261 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 320
            M+ARGDLGMEIP EK+FLAQKMMI KCN+AGKPV+TATQMLESM K+PRPTRAEA DVA
Sbjct: 297 IMIARGDLGMEIPPEKVFLAQKMMISKCNVAGKPVITATQMLESMTKNPRPTRAEAADVA 356

Query: 321 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRSTPLPMSPL 380
           NAVLDGTDCVMLSGE+A GS+P  AV +M+R+C EAE  +DY  +F+   ++T  P+   
Sbjct: 357 NAVLDGTDCVMLSGETANGSFPVQAVTVMSRVCFEAEGCIDYQQVFRATCQATMTPIDTQ 416

Query: 381 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWSCSD 440
           E++A +AV TA    A LI+ LT  G TA+L+AKYRP  PIL++            S S+
Sbjct: 417 EAVARAAVETAQSINASLILALTETGRTARLIAKYRPMQPILAL------------SASE 476

Query: 441 ESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCKPGDAIVALHRI- 500
           E+  +  +I RG+   L          + T+ ++  AL +A    L   GD+IVA+H I 
Sbjct: 477 ETIKQLQVI-RGVTTFL------VPTFQGTDQLIRNALSAAKELQLVSEGDSIVAVHGIK 531

Query: 501 ----GAASVIKICIVK 511
               G ++++K+ +V+
Sbjct: 537 EEVAGWSNLLKVLVVE 531

BLAST of Lsi04G007690 vs. ExPASy TrEMBL
Match: A0A5A7SUF8 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003300 PE=3 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 8.8e-282
Identity = 500/510 (98.04%), Postives = 506/510 (99.22%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD EAGRV+CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRKILGPHAKHIKLMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIR+TPLPMSPLESLASSAVRTANKA+AKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKARAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. ExPASy TrEMBL
Match: A0A0A0KV32 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_5G580610 PE=3 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 8.8e-282
Identity = 499/510 (97.84%), Postives = 506/510 (99.22%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD EAGRV+CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PG+VVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRK+LGPHAKHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIR+TPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. ExPASy TrEMBL
Match: A0A1S3BDT2 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103488792 PE=3 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 8.8e-282
Identity = 500/510 (98.04%), Postives = 506/510 (99.22%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD EAGRV+CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRKILGPHAKHIKLMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIR+TPLPMSPLESLASSAVRTANKA+AKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKARAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. ExPASy TrEMBL
Match: A0A218KBQ1 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=PK32 PE=2 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 1.1e-279
Identity = 496/510 (97.25%), Postives = 503/510 (98.63%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD EAGRV+CRCENTAMLGERKN  L
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNXXL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PG+VVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRK+LGPHAKHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIR+TPLPMSPL SLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLXSLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. ExPASy TrEMBL
Match: A0A6J1CAX5 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111009998 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.5e-278
Identity = 494/510 (96.86%), Postives = 503/510 (98.63%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGNTILCSDGTITLTVLSCD+  G V CRCENTAMLGERKNVNL
Sbjct: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLVNVRK+LGPH+KHIKLMSK
Sbjct: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGVIFKEMIR+TPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. NCBI nr
Match: XP_038892449.1 (pyruvate kinase, cytosolic isozyme [Benincasa hispida] >XP_038892452.1 pyruvate kinase, cytosolic isozyme [Benincasa hispida])

HSP 1 Score: 985.7 bits (2547), Expect = 1.5e-283
Identity = 505/510 (99.02%), Postives = 508/510 (99.61%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN+ILCSDGTITLTVLSCD EAGRVICRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNSILCSDGTITLTVLSCDPEAGRVICRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PG+VVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRKILGPHAKHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. NCBI nr
Match: XP_008445909.1 (PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0034093.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa] >TYK15825.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa])

HSP 1 Score: 978.8 bits (2529), Expect = 1.8e-281
Identity = 500/510 (98.04%), Postives = 506/510 (99.22%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD EAGRV+CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRKILGPHAKHIKLMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIR+TPLPMSPLESLASSAVRTANKA+AKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKARAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. NCBI nr
Match: XP_004147096.1 (pyruvate kinase, cytosolic isozyme [Cucumis sativus] >XP_031741666.1 pyruvate kinase, cytosolic isozyme [Cucumis sativus] >KGN51576.1 hypothetical protein Csa_008390 [Cucumis sativus])

HSP 1 Score: 978.8 bits (2529), Expect = 1.8e-281
Identity = 499/510 (97.84%), Postives = 506/510 (99.22%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD EAGRV+CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PG+VVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRK+LGPHAKHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIR+TPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. NCBI nr
Match: AKN59232.1 (pyruvate kinase [Cucumis sativus])

HSP 1 Score: 971.8 bits (2511), Expect = 2.2e-279
Identity = 496/510 (97.25%), Postives = 503/510 (98.63%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           EEMISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD EAGRV+CRCENTAMLGERKN  L
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNXXL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PG+VVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLV VRK+LGPHAKHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIR+TPLPMSPL SLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLXSLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLCKPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. NCBI nr
Match: XP_022138945.1 (pyruvate kinase, cytosolic isozyme [Momordica charantia])

HSP 1 Score: 966.5 bits (2497), Expect = 9.3e-278
Identity = 494/510 (96.86%), Postives = 503/510 (98.63%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGNTILCSDGTITLTVLSCD+  G V CRCENTAMLGERKNVNL
Sbjct: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLVNVRK+LGPH+KHIKLMSK
Sbjct: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGVIFKEMIR+TPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+CSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510

BLAST of Lsi04G007690 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 892.1 bits (2304), Expect = 2.1e-259
Identity = 449/510 (88.04%), Postives = 485/510 (95.10%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           M+NIDIEGILKELPNDGRIPKTKIVCTLGPASR+V MIEKLL+AGMNVARFNFSHG+HEY
Sbjct: 1   MSNIDIEGILKELPNDGRIPKTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETL+NLR+AM NT IL AVMLDTKGPEIRTGFLKDG PIQLKEGQE+ ITTDY I+GD
Sbjct: 61  HQETLDNLRSAMHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           E  ISMSY+KL +D+KPGNTILC+DG+I+L VLSCD E+G V CRCEN+AMLGERKNVNL
Sbjct: 121 ESTISMSYKKLPLDVKPGNTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLT+KD EDILGWGVPNSIDMIALSFVRKGSDLVNVRK+LG HAK I LMSK
Sbjct: 181 PGVVVDLPTLTDKDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLGSHAKSIMLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIP+EKIFLAQK+MIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAG+YPE+AVK+MA+ICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKVMAKICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           Y  IFKEMIR+TPLPMSPLESLASSAVRTANKA+AKLI+VLTRGG+TA LVAKYRPAVPI
Sbjct: 361 YNTIFKEMIRATPLPMSPLESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPV+TTDSFDWSCSDESPARHSLI+RGLIP+LAEGSAKATD+E+TEVI+EAALKSA
Sbjct: 421 LSVVVPVMTTDSFDWSCSDESPARHSLIYRGLIPMLAEGSAKATDSEATEVIIEAALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
             +GLC  GDAIVALHRIGAASVIKIC+VK
Sbjct: 481 TQRGLCNRGDAIVALHRIGAASVIKICVVK 510

BLAST of Lsi04G007690 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 887.9 bits (2293), Expect = 3.9e-258
Identity = 448/510 (87.84%), Postives = 481/510 (94.31%), Query Frame = 0

Query: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           M+NIDIEGILKELPNDGR PKTKIVCTLGPASRSV MIEKLL+AGMNVARFNFSHG+HEY
Sbjct: 1   MSNIDIEGILKELPNDGRTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEY 60

Query: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETL+NLRTAMQNT IL AVMLDTKGPEIRTGFLKDG PIQLKEGQE+ ITTDY IKGD
Sbjct: 61  HQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGD 120

Query: 121 EEMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNL 180
           E+ ISMSY+KL VD+KPGNTILC+DG+I+L V+SCD  AG VICRCENTAMLGERKNVNL
Sbjct: 121 EKTISMSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSK 240
           PGVVVDLPTLT+KD EDIL WGVPN+IDMIALSFVRKGSDLVNVRK+LG H+K I LMSK
Sbjct: 181 PGVVVDLPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHSKSIMLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGV+NFDEILRETDAFMVARGDLGMEIP+EKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAG+YPE+AVK MA+ICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAESSLD 360

Query: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           Y  IFKEMIR+TPLPMS LESLASSAVRTANKAKAKLI+VLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YNTIFKEMIRATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWSCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPV T+D+F+WSCSDESPARHSLI+RGLIP+L EGSAKATD+ESTE I+E+ALKSA
Sbjct: 421 LSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSA 480

Query: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
             KGLC  GDA+VALHRIGAASVIKIC+VK
Sbjct: 481 TEKGLCNHGDAVVALHRIGAASVIKICVVK 510

BLAST of Lsi04G007690 vs. TAIR 10
Match: AT5G56350.1 (Pyruvate kinase family protein )

HSP 1 Score: 771.5 bits (1991), Expect = 4.1e-223
Identity = 389/491 (79.23%), Postives = 435/491 (88.59%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRTAMQNTQILC 79
           PKTKIVCTLGPASRSVPM+EKLLRAGMNVARFNFSHG+HEYHQETL+NL  AM NT ILC
Sbjct: 8   PKTKIVCTLGPASRSVPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLHQAMLNTGILC 67

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKDGKPIQLK+GQE+ I+TDY +KGDE  I MSY+KLAVD+ PG 
Sbjct: 68  AVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDENTICMSYKKLAVDVNPGM 127

Query: 140 TILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNLPGVVVDLPTLTEKDKEDIL 199
            ILC+DGTI+L VLSCD E G V CRCEN+AMLGERKNVNLPGVVVDLPTLTEKDKEDI+
Sbjct: 128 VILCADGTISLLVLSCDKENGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTEKDKEDIM 187

Query: 200 GWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSKVENQEGVINFDEILRETDA 259
            WGVPN IDMIALSFVRKGSDLV VRK+LG HAK+I LMSKVENQEGV NFD+IL  +DA
Sbjct: 188 QWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKNILLMSKVENQEGVANFDDILVNSDA 247

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FM+ARGDLGMEIP+EKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 248 FMIARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 307

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRSTPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YPELAV+ MA+IC+EAES+LDYG +FK ++  +P+PMSPL
Sbjct: 308 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMLYSPVPMSPL 367

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWSCSD 439
           ESLASSAVRTAN A+A LI+VLTRGG+TA+LVAKYRP +PILSVVVP + TD FDWSCSD
Sbjct: 368 ESLASSAVRTANSARATLIMVLTRGGSTARLVAKYRPGMPILSVVVPEIKTDFFDWSCSD 427

Query: 440 ESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCKPGDAIVALHRIG 499
           ESPARHSLI RGLIP+L  GSA+A+  ESTE  +E A +    K LCK GD++VAL R+G
Sbjct: 428 ESPARHSLIFRGLIPVLYAGSARASHDESTEEAIEFATQYGKEKELCKTGDSVVALLRVG 487

Query: 500 AASVIKICIVK 511
            ASVIKI  VK
Sbjct: 488 NASVIKILTVK 498

BLAST of Lsi04G007690 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 751.1 bits (1938), Expect = 5.7e-217
Identity = 378/491 (76.99%), Postives = 431/491 (87.78%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRTAMQNTQILC 79
           PKTKIVCTLGPASRSVPM+EKLL AGM+VARFNFSHG++EYHQETL+NLR AM NT +LC
Sbjct: 7   PKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLC 66

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKDGKPIQLK+GQE+ I+TDY +KGDE+ I MSY+KLA D+ PG 
Sbjct: 67  AVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPGM 126

Query: 140 TILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNLPGVVVDLPTLTEKDKEDIL 199
            ILC+DGTI+L VLSCD E G V CRCENT+MLGERKNVNLPGVVVDLPTLTEKDK+DIL
Sbjct: 127 VILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEKDKQDIL 186

Query: 200 GWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSKVENQEGVINFDEILRETDA 259
            WGVPN IDMIALSFVRKGSDLV VRK+LG HAK I LMSKVENQEGV NFD+IL  +DA
Sbjct: 187 EWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDILINSDA 246

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FM+ARGDLGMEIP+EKIFLAQK+MIYKCN  GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 247 FMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRAEATDVA 306

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRSTPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YPELAV+ MA+IC+EAES+LDYG IFK ++    +PMSP+
Sbjct: 307 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAAVPMSPM 366

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWSCSD 439
           ESLASSAVRTA  ++A L++VLTRGG+TA+LVAKYRP +PILSVVVP +T+DSFDW+CS+
Sbjct: 367 ESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSFDWACSN 426

Query: 440 ESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCKPGDAIVALHRIG 499
           E+PARHSLI+RGL+P+L  GSA+A+  ESTE  LE A +    K LCK GD++VAL R G
Sbjct: 427 EAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVVALFRTG 486

Query: 500 AASVIKICIVK 511
            A VIKI  VK
Sbjct: 487 NAIVIKILTVK 497

BLAST of Lsi04G007690 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 683.7 bits (1763), Expect = 1.1e-196
Identity = 352/510 (69.02%), Postives = 425/510 (83.33%), Query Frame = 0

Query: 6   IEGILKELPNDGRI-PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQET 65
           +E +L    N+G +  KTKIVCTLGPASRSV M+EKLL+AGMNVARFNFSHG+H YHQET
Sbjct: 1   MEKLLAGQTNNGSLKSKTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQET 60

Query: 66  LNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEEMI 125
           L+NLRTAM+NT I CAVMLDTKGPEIRTGFLK+GKP++L +GQE+ I+TDY+++GD   I
Sbjct: 61  LDNLRTAMENTCIPCAVMLDTKGPEIRTGFLKEGKPVELIQGQEITISTDYTMEGDSNTI 120

Query: 126 SMSYQKLAVDLKPGNTILCSDGTITLTVLSCDSEAGRVICRCENTAMLGERKNVNLPGVV 185
           SMSY+KLA DLK G+ ILCSDGTI+LTVLSCD   G V  RCEN+A+LGERKNVNLPG+V
Sbjct: 121 SMSYKKLAEDLKSGDVILCSDGTISLTVLSCDKNLGLVRARCENSAVLGERKNVNLPGIV 180

Query: 186 VDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVNVRKILGPHAKHIKLMSKVENQ 245
           VDLPTLTEKD+EDIL WGVPN ID+IALSFVRKGSDLV VRK+LG +AK I LMSKVENQ
Sbjct: 181 VDLPTLTEKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRKLLGENAKSIMLMSKVENQ 240

Query: 246 EGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESM 305
           EGV+NFD+IL  +DAFMVARGDLGMEIP+EK+FLAQKMMI K N  GKP+VTATQMLESM
Sbjct: 241 EGVMNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESM 300

Query: 306 IKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVI 365
            KSPRPTRAEATDVANAVLDGTDCVMLSGE+AAG++PE AV  M+RIC EAE  +DY  +
Sbjct: 301 TKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDTM 360

Query: 366 FKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVV 425
            K++     LP+SP+ESLA+SAV TA    A  IVVLT+GG T +LVAKYRP+VPILSV+
Sbjct: 361 HKKIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVI 420

Query: 426 VPVLT-TDSFDWSCSDESP--ARHSLIHRGLIPILAEG-SAKATDAESTEVILEAALKSA 485
           VP +T TD F+WSCS+ +   AR  LI+RG++P++A G SA++++ +STE  ++ A++ A
Sbjct: 421 VPEITRTDDFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFAIEFA 480

Query: 486 MGKGLCKPGDAIVALHRIGAASVIKICIVK 511
             KG+CK GD+IVALH+I  +SV+KI  V+
Sbjct: 481 KKKGICKTGDSIVALHKIDGSSVVKILNVE 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q428066.5e-26691.98Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1[more]
P222001.7e-26189.22Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q429541.0e-22379.23Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O655958.0e-21676.99Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=A... [more]
O440061.7e-12549.40Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SUF88.8e-28298.04Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00330... [more]
A0A0A0KV328.8e-28297.84Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_5G580610 PE=3 SV=1[more]
A0A1S3BDT28.8e-28298.04Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103488792 PE=3 SV=1[more]
A0A218KBQ11.1e-27997.25Pyruvate kinase OS=Cucumis sativus OX=3659 GN=PK32 PE=2 SV=1[more]
A0A6J1CAX54.5e-27896.86Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111009998 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038892449.11.5e-28399.02pyruvate kinase, cytosolic isozyme [Benincasa hispida] >XP_038892452.1 pyruvate ... [more]
XP_008445909.11.8e-28198.04PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0034093.1 pyruv... [more]
XP_004147096.11.8e-28197.84pyruvate kinase, cytosolic isozyme [Cucumis sativus] >XP_031741666.1 pyruvate ki... [more]
AKN59232.12.2e-27997.25pyruvate kinase [Cucumis sativus][more]
XP_022138945.19.3e-27896.86pyruvate kinase, cytosolic isozyme [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G08570.12.1e-25988.04Pyruvate kinase family protein [more]
AT5G63680.13.9e-25887.84Pyruvate kinase family protein [more]
AT5G56350.14.1e-22379.23Pyruvate kinase family protein [more]
AT4G26390.15.7e-21776.99Pyruvate kinase family protein [more]
AT3G04050.11.1e-19669.02Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 77..93
score: 62.9
coord: 333..349
score: 54.03
coord: 264..288
score: 64.86
coord: 314..332
score: 77.25
coord: 289..313
score: 74.58
coord: 237..263
score: 52.54
coord: 207..221
score: 51.79
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 21..497
e-value: 3.9E-158
score: 524.8
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 1..510
IPR001697Pyruvate kinaseCDDcd00288Pyruvate_Kinasecoord: 19..509
e-value: 0.0
score: 733.349
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 88..188
e-value: 8.4E-197
score: 656.3
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 356..497
e-value: 8.4E-197
score: 656.3
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 344..509
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 21..355
e-value: 8.4E-197
score: 656.3
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 20..361
e-value: 2.9E-159
score: 529.6
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 380..502
e-value: 5.0E-22
score: 78.3
NoneNo IPR availablePANTHERPTHR11817:SF87PYRUVATE KINASEcoord: 1..510
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 235..247
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 88..187
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 16..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G007690.1Lsi04G007690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity